<p>Mutations in the gene SHANK2, which encodes a synaptic scaffolding protein, have been shown to cause a spectrum of neuropsychiatric disorders including: intellectual disability, autism spectrum disorders (ASDs), bipolar disorder (BD), and schizophrenia. However, many aspects of SHANK2 including the array of isoforms expressed, the expression pattern of the protein, biochemical and regulatory mechanisms, and in vivo protein function remain elusive. This body of work aims to uncover the function of the SHANK2 gene and its role in neuropsychiatric disorders using in vitro and in vivo experimental systems.</p> <p>Using a molecular genetics approach, I revealed the transcript architecture of the mouse Shank2 gene including characterization of promoters, isoforms and protein domains. I then outlined the temporal and spatial pattern of the Shank2 isoform expression throughout development. To further explore the protein’s function, we sought to identify novel SHANK2 interacting proteins using a yeast-2-hybrid screen and characterized the interacting proteins. Lastly, in order to understand how Shank2 deficiencies alter brain function we generated and characterized both Shank2 conventional (∆e24) and conditional mutant mice (e24floxed) by deleting or floxing exon 24 that encodes the Homer binding site and has nonsense mutations in human patients with neuropsychiatric disorders.</p> <p>Collectively, these studies 1) provide insight into the transcriptional regulation of Shank2 during brain development; 2) support the value of using Shank2 to further dissect the pathophysiology and circuitry mechanism underlying manic and autism like behaviors; 3) offers a novel mechanistic link between ubiquitination-mediated protein modification and SHANK2 function that may elucidate the molecular basis underlying SHANK2-related neuropsychiatric disorders. Ultimately, these findings may lead to the development of new therapeutic interventions for SHANK2-related neuropsychiatric disorders.</p> / Dissertation

El propósito de este estudio es describir la Calidad de vida y Funcionalidad mediante test de valoración de dichas cualidades (SF-36 y Score Funcional Cadera de D`Aubigne y Postel ) en 8 pacientes intervenidos quirúrgicamente en los servicios de Urgencia y Traumatología del Hospital Clínico de la Universidad de Chile, en los que se realizó una fijación interna mediante la técnica de osteosíntesis DHS, entre agosto del 2003 y mayo del 2005. Paralelamente se realizaron las mismas evaluaciones en un grupo control integrado por 9 adultos mayores. / The purpose of this study is to describe the Quality of life and hip Functionality using valuation tests of such qualities (SF-36 and D´ Aubigne and Postel Hip functional Score ) in 8 patients who were put under surgery product of a extracapsular hip fracture using the internal fixation method DHS in Universidad de Chile Clinical Hospital between August of the 2003 and May of the 2005. In parallel the same evaluations adults were carried out in a group control comprising 9 older adults.

Made available in DSpace on 2015-04-14T14:33:17Z (GMT). No. of bitstreams: 1 397632.pdf: 131587 bytes, checksum: 42385c7767b31c251aa7402c110b31d2 (MD5) Previous issue date: 2007-12-04 / Este trabalho, vinculado ? linha de pesquisa instrumentalidade e efetividade do processo civil, analisa o princ?pio da isonomia no caso dos servidores p?blicos estatut?rios, especificadamente em face do julgamento de improced?ncia de uma a??o individual e de proced?ncia de uma a??o coletiva, quando id?ntico o objeto de ambas as a??es. Sustenta que, por se tratar do reconhecimento do direito de uma categoria direito coletivo stricto sensu e por possuir v?nculo de natureza jur?dica institucional com a Administra??o P?blica, reclama id?ntico tratamento jur?dico, o qual ser? alcan?ado a partir da preponder?ncia do resultado da a??o coletiva. Explica que as diversas interpreta??es da lei, respons?veis pelas decis?es divergentes, n?o deve ser admitida quando se reconhece o direito aos servidores p?blicos estatut?rios em a??o coletiva, uma vez que afronta o princ?pio da isonomia. Para a preponder?ncia do resultado e a devida extens?o ao titular do direito individual, o ?bice da coisa julgada, para alcan?ar a isonomia, ? superado a partir da aplica??o do princ?pio da proporcionalidade. Recorre ? an?lise doutrin?ria, jurisprudencial e legislativa, em especial a nacional.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-03-27T18:25:02Z No. of bitstreams: 1 TES_BARBARA_BURGARDT_CASALETTI_COMPLETO.pdf: 767639 bytes, checksum: 62ca192e9ae9e952b07c31afc62b9efe (MD5) / Made available in DSpace on 2017-03-27T18:25:03Z (GMT). No. of bitstreams: 1 TES_BARBARA_BURGARDT_CASALETTI_COMPLETO.pdf: 767639 bytes, checksum: 62ca192e9ae9e952b07c31afc62b9efe (MD5) Previous issue date: 2017-01-18 / This thesis was elaborated in the research line Education, Policies and Practices in Education of the Program of Post-Graduation in Education of PUCRS and investigated how the evaluation of training and improvement actions in a corporate education context is being carried out. Its construction is inspired by the Resolutions of the National Council of Justice ? CNJ ? and the Superior Council of Labor Justice - CSJT, which deal with the national policy of training and perfecting the servants of the Judiciary, as well as the national policy of education for servers of Labor Justice of first and second degree. In the process of data collection, the interview was used, which, according to Severino (2013), provides an interaction between the researcher and his interlocutors. The respondents were chosen intentionally within the universe investigated and the approach of the interviews (semi-structured) and the other data collected were submitted to discursive textual analysis, according to terms proposed by Moraes and Galliazzi (2011), the following categories being previously defined were: 1?) assessment and reaction; 2?) learning assessment; 3?) application assessment; 4?) outcome assessment. Those categories were chosen according to the content of the aforementioned Resolutions, where it is expected that ?training and improvement actions will be evaluated, whenever possible, by the organs of the judiciary, to guide new decision-making and the specificity of the Formative action in four dimensions: reaction, learning, application and outcome? (Resolution CNJ n? 192/2014 and Resolution CSJT n? 159/2015). In addition, the studies of Kirkpatrick and Kirkpatrick (2010a, 2010b) were also considered. The narrative as method was assumed, in the terms proposed by Clandinin and Connelly (2000), where the temporality and the stories lived and narrated are considered. / Esta tese foi elaborada na linha de pesquisa Forma??o, Pol?ticas e Pr?ticas em Educa??o do Programa de P?s-Gradua??o em Educa??o da PUCRS e investigou como est? sendo realizada a avalia??o das a??es de forma??o e aperfei?oamento em um contexto de educa??o corporativa. Sua constru??o inspira-se no contido em Resolu??es do Conselho Nacional de Justi?a ? CNJ e do Conselho Superior da Justi?a do Trabalho - CSJT, que tratam da pol?tica nacional de forma??o e aperfei?oamento dos servidores do Poder Judici?rio, bem como da pol?tica nacional de educa??o para servidores da Justi?a do Trabalho de primeiro e segundo graus. No processo de coleta de dados, foi utilizada a entrevista, que, de acordo com Severino (2013), proporciona uma intera??o entre pesquisador e pesquisado. Os respondentes foram escolhidos intencionalmente dentro do universo investigado e a abordagem do presente estudo foi qualitativa, onde as respostas ?s quest?es das entrevistas (semiestruturadas) e os demais dados coletados foram submetidos ? an?lise textual discursiva, nos termos propostos por Moraes e Galliazzi (2011), onde foram previamente definidas as seguintes categorias: 1?) avalia??o de rea??o; 2?) avalia??o de aprendizagem; 3?) avalia??o de aplica??o; 4?) avalia??o de resultado. Referidas categorias foram escolhidas em fun??o do teor das Resolu??es antes mencionadas, onde existe a previs?o de que as ?a??es de forma??o e aperfei?oamento sejam avaliadas, sempre que poss?vel, pelos ?rg?os do Poder Judici?rio, para orientar novas tomadas de decis?es e observada a especificidade da a??o formativa, em quatro dimens?es: rea??o, aprendizagem, aplica??o e resultado? (Resolu??o CNJ n? 192/2014 e Resolu??o CSJT n? 159/2015). Al?m disso, os estudos de Kirkpatrick & Kirkpatrick (2010a; 2010b) tamb?m foram considerados. A narrativa como m?todo foi assumida, nos termos propostos por Clandinin e Connelly (2000), onde a temporalidade e as hist?rias vividas e narradas s?o observadas.

SUMMARY Lithuanian army physical training mission-is to form a healthy, strong, well physically prepared soldier, ready to fulfil all raised tactical assignments. The aim of this research was to evaluate the endurance of soldiers, developing the cardiovascular function abilities in performing of aerobic loads. Investigation was done in Kaunas city “Vytautas Magnus Keepers” battalion stadium. The request was given and the permission of the battalion commander was received in order to perform the biomedical research. Six athletes elevating high technique endurance participated in this research. The participants voluntarily agreed to perform the testing. All the participants (soldiers and runners) after the individual warming-up, ran a 5km cross-country (the task of the exercise was endurance training: even running in an aerobic zone). During the second research the runners were running a 12km cross-country (the task of the exercise was endurance training: even running in an aerobic zone). Using the heart rate (HR) pulse monitor “Polar-S810” The changes of HR was measured (the instantaneous account of HR and the average (HRmax), an account of the chosen length (each 1000m) and the duration (t) of chosen running length (each 1000m running length). The index of biological load value was counted of those registered indicators. In the third research the participants in LKKA laboratory of Kinesiology performed the search of qualification: the test of Roufier physical load, while... [to full text]

The objective of this thesis is to evaluate the possibility of creating a tool capable of predicting commodity prices. Along with other business strategies, tools and markets analyses for financial and capital markets, this tool should help make the best estimate of future developments on the observed markets. The main market, on which this work is focused, is the agricultural commodities market, namely corn and its related markets. The fundamental basis upon which the arguments in this thesis are built, is the use of artificial intelligence, particularly neural networks. The whole application is presented using a graphical user interface that allows even those with little or no understanding of this field to delve deeper into the interesting area - using modern computer systems to support trading activities.

Este estudio tiene por finalidad observar, describir y analizar como se comportan ciertos parámetros hemodinámicos y ventilatorios en un grupo de pacientes críticos al aplicarles un Test de Tolerancia a la Verticalización (TTV). Las mediciones se realizaron en un grupo de 51 pacientes pertenecientes a la Unidad de Pacientes Críticos del Hospital Clínico de la Universidad de Chile, durante los meses de Junio a Octubre del 2005. La prueba que se aplicó a estos pacientes consistió en realizar la primera sedestación después de su ingreso al Hospital, al borde de la cama durante seis minutos y medir su frecuencia cardiaca, presión arterial sistólica, presión arterial diastólica, presión arterial media, frecuencia respiratoria, saturación de oxígeno, y sensación subjetiva de fatiga. Estas mediciones se realizaron en el minuto cero (reposo), uno, tres y seis de la prueba. / This study has been made for watching, describe, and analize the behaviour of hemodinamics and ventilatory measurements in a group of critical patients at moment of applyng the Verticalitation Tolerance Test (VTT). The measurements were made in a group of 51 patients belong of the Critical Patients Unit (CPU) of the Clinical Hospital of Universidad de Chile, between months of June and October 2005. The test applied consist in sitting for first time, after the entry to this unit, in the edge of bed during six minutes period and measure their cardiac rates, systolic arterial pressures, dyastolic arterial pressures, mean arterial pressures, respiratory rates, oxygen saturate and fatigue subjective sensation. The measurement was made in one, three and six minutes of the test.

La réflexion proposée prend forme à partir d’un projet de vie établi entre l’Equateur et la France. La thèse interroge les conditions de conduite du travail juridique dans l’orbite du phénomène de territorialisation des ‘établissements humains informels’ -asentamientos urbanos informales- de Guayaquil, ailleurs connus comme ‘slums’ ou ‘taudis’. La mise en cause de l’incidence économique, sociale, culturelle ou environnementale des usages et pratiques professionnelles du droit sur la ville en devenir, l’évocation d’une dimension éventuellement marginalisante, discriminatoire ou traumatique du travail juridique depuis la perspective des ‘usagers’ ou des ‘administrés’, paraissent troublantes au premier abord. Inscrits dans un mouvement d’itération constante entre l’observation participante et la théorie de l’agir juridique, les travaux entreprennent de poser les jalons épistémologiques et méthodologiques pour un travail de situation de l’agir juridique aux portes de la ville Sud. La lecture renouvelée de la contextualité urbaine marginale et le développement d’instruments de cartographie de la fonction sociale du juriste ‘en situation’ constituent les principaux résultats de l’investigation. Les développements coïncident, par ailleurs et d’une manière générale, avec l’investiture du gouvernement de la « Révolution citoyenne » -Revolucion ciudadana-. Ils offrent, dès lors et chemin faisant, un aperçu « vu d’en bas » sur le devenir des réformes traversant le pays aux lendemains de l’adoption de la Constitution du « Buen vivir ». / The investigation is building upon a life project established between Ecuador and France. The doctoral thesis questions social legal work’s conditions in the ambit of Guayaquil’s informal human settlements’ territorialization -elsewhere known as “slums” or “taudis”-. The mention of economic, social, cultural or environnemental’s downfalls associated to legal practices’ development in forthcoming cities, the pointing out of ongoing discriminations, marginalization and traumas caused by legal malpractices does create, at first glimpse, an awkward feeling among the legal development practitioners’ community. Through a continued iteration between participative observation, fieldworks and legal endeavor’s theory, the doctoral thesis develops epistemological and methodological backgrounds in order to better situate legal work in city peripheries of the South. Means to renew our understandings of marginal urban contexts, on the one hand, and to reevaluate the social function of legal practices, on the other, are the principal outcomes of our research work. The fieldwork coincided, otherwise, with the investiture of the “Revolucion ciudadana government”. Hence, the thesis does offer some bottom-up insights on the becomings of legal and political reforms in the aftermaths of the “Buen vivir” Ecuadorian Constitution’adoption.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-06-11T18:16:21Z No. of bitstreams: 1 470251 - Texto Parcial.pdf: 476694 bytes, checksum: 59888ba9784d932d1d6abf6f8133a3ef (MD5) / Made available in DSpace on 2015-06-11T18:16:21Z (GMT). No. of bitstreams: 1 470251 - Texto Parcial.pdf: 476694 bytes, checksum: 59888ba9784d932d1d6abf6f8133a3ef (MD5) Previous issue date: 2015-01-15 / This research aims to analyze the possibilities and the boundaries of application of criminal law for the protection of economic, specifically in the case of corrupt practices, due to the finding of corruption as widespread global phenomenon and negative effects on national and international markets. By addressing historical origins of corruption in Brazil and its current aspects, studies the international anti-corruption initiatives and their impact on the Brazilian legal system of control of corruption, seeing the economic order and social interest such as to protection through criminal law. / Este trabalho pretende analisar as possibilidades do recurso ao direito penal como instrumento de prote??o da ordem econ?mica, vislumbrando seus limites para consecu??o do objetivo de controle social, em raz?o da expans?o do uso da resposta penal na contemporaneidade, especificamente no caso da corrup??o. A partir do reconhecimento do car?ter complexo das rela??es sociais na atualidade, e tomando consci?ncia dos efeitos da globaliza??o sobre a nova ordem mundial, busca-se compreender a corrup??o n?o mais como problema do trato interno dos pa?ses, mas que assumiu na atualidade a fei??o de fen?meno global, cujos efeitos nefastos podem transcender as fronteiras e atingir a sociedade em todos os seus aspectos, inclusive o econ?mico. A constata??o da onipresen?a do fen?meno corruptivo nas sociedades organizadas, e seus efeitos sobre a economia, leva a uma rea??o da comunidade internacional, que passa a envidar esfor?os para a ado??o de meios jur?dicos para enfrentamento do problema, com a celebra??o de Tratados Anticorrup??o. Ao buscar posicionamento no cen?rio internacional, o Brasil se alinha a tais iniciativas, recebendo o impacto da assun??o das obriga??es e mandados previstos nas Conven??es, inclusive com a incrimina??o de condutas aptas a atingir a higidez de neg?cios internacionais, como no crime de corrup??o de agentes p?blicos estrangeiros, previsto no artigo 337-B do C?digo Penal. Os instrumentos jur?dicos internacionais somam-se aos mecanismos c?veisadministrativos e criminais de repress?o ?s pr?ticas corruptivas, conformando o sistema jur?dico-brasileiro de controle da corrup??o. A partir desta an?lise, abarca ao estudo o controle penal como meio de prote??o da ordem econ?mica, e o reconhecimento da mesma como bem jur?dico penalmente digno de relev?ncia, a partir do assento constitucional recebido na Constitui??o Federal de 1988. Analisa-se a partir de ent?o a repress?o ? corrup??o por meio do direito penal secund?rio, que tutela n?o apenas bens jur?dicos de car?ter individual, mas tamb?m aqueles cuja les?o pode atingir interesses difusos, coletivos, imposs?veis de individualizar, sendo os limites e possibilidades da tutela penal ? ordem econ?mica avaliados e discutidos.

Made available in DSpace on 2014-12-17T15:38:55Z (GMT). No. of bitstreams: 1 MonicaOL.pdf: 324604 bytes, checksum: 4dab56b1c8ef62feb374b553f7aba19b (MD5) Previous issue date: 2006-10-24 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Currently, many companies pay attention to the necessities of change of attitude in relation to the use of the natural resources and the environment quality of its products, mainly to keep a positive and competitive image in the market. This new concept of management associates the productive activities to the care with environment using the education of environment as a tool of programs of qualification directed toward the promotion of pro-environment behaviors. For effectiveness of this management it is essential the attachment of the employees and the major understanding of the importance of the preservation of the environment. This was the objective of the qualification process carried out in a lodge in Fernando de Noronha island. The purpose of this study, therefore, was to verify the power of this management system which fulfills the objectives of the environmental education. It was applied questionnaires to employees of three Inns and carried out a interview, in one Inn, with the participants of the program of qualification. The analysis of the data showed that the actions as care with the environment are strongly associates to the management system, which includes garbage control, as well water economy. It was observed that the environment accomplishment (EcoAntro) of the employees with environment decreases in proportion to their qualification. In the same way, as higher the instruction level, of the speaker and/or of the father, less is the apathy for environmental questions. It was also noticed that the knowledge about the development sustainability was very poorly used by the interviewed. The pro-environment behavior mentioned before, is not necessarily related to a local autonomy posture that could express a behavior related to job but not to environment it self. Therefore, a complete understanding about the reverberation of environment information an all the placed factors in a life stile facing a construction of sustainability remains as a question to be studied in future researches / Atualmente, muitas empresas atentam para as necessidades de mudan?a de postura em rela??o ? utiliza??o dos recursos naturais e a qualidade ambiental de seus produtos, principalmente para manter uma imagem positiva e competitiva no mercado. Esse novo conceito de gerenciamento associa as atividades produtivas ao cuidado com ambiente utilizando a educa??o ambiental como ferramenta de programas de capacita??o voltados para a promo??o de comportamentos pr?-ambientais. Para efic?cia desse gerenciamento ? imprescind?vel o envolvimento dos funcion?rios e a compreens?o maior da import?ncia da preserva??o do meio ambiente. Esse foi o objetivo do processo de capacita??o realizado por uma pousada em Fernando de Noronha. O prop?sito deste estudo, portanto, foi verificar o alcance deste sistema de gest?o em atender aos objetivos da educa??o ambiental. Foram aplicados question?rios a funcion?rios de tr?s pousadas e realizadas entrevistas com os participantes do programa de capacita??o de uma delas. A an?lise dos dados evidenciou que as a??es de cuidado ambiental est?o bastante associadas ao sistema de gest?o, que incluem controle de lixo, e tamb?m a economia de ?gua. Foi observado que o ambientalismo (EcoAntro) dos funcion?rios decresce ? medida que ? menor o seu envolvimento com o programas de capacita??o. Da mesma forma, quanto maior o n?vel de instru??o, do pr?prio respondente e/ou do pai, menor a apatia por quest?es ambientais. Tamb?m foi poss?vel observar que a no??o de desenvolvimento sustent?vel ? pouco dominada pelos participantes. Os comportamentos pr?-ambientais mencionados n?o est?o necessariamente relacionados a uma postura sustent?vel, podendo expressar um comprometimento com o trabalho, mas n?o com o meio ambiente. Desse modo, o pleno entendimento da reverbera??o da informa??o ambiental e dos fatores situacionais em um estilo de vida direcionado para a constru??o da sustentabilidade permanece como quest?o a ser estudada em pesquisas futuras