Bakgrund och problem: Det som står klart tidigt i problematiseringen och bakgrunden är vikten av att utveckla adekvat ekonomistyrning när organisationer växer. Med utgångspunkt i att ekonomistyrning utvecklas i takt med att företag växer problematiseras kring ämnet och hur det påverkar SMEs i stark tillväxt. Syfte: Syftet med uppsatsen är att öka och fördjupa förståelsen för hur ekonomistyrningen utvecklas i snabbt växande företag. Syftet är därtill att identifierade kritiska händelser och faktorer som skett under tillväxtresan som gett upphov till förändringar i ekonomistyrningen. Metod: Studien är av en kvalitativ karaktär i form av en flerfallsstudie. Den primära datainsamlingen har skett med hjälp av semistrukturerade intervjuer. Intervjuer har genomförts med såväl ledande befattningshavare som medarbetare för att skapa en uppfattning kring hur styrningen har utvecklats över tid i de båda fallföretagen. Slutsats: Det som karaktäriserar ekonomistyrningen i fallföretagen är dels en rad kritiska händelser som påverkat beslut och framtida utveckling av företagen. Därtill har både externa och interna effekter varit bidragande till de kritiska händelserna och utvecklingen av företagen. Avslutningsvis har företagen agerat för att anpassa ekonomistyrningen i takt med att de har haft en snabb tillväxt, ett arbete som genomgående präglats av en successiv utveckling för att möta de behov som uppstått. / Background: What is clear early in the problem discussion and background is the importance of developing adequate management accounting when organizations grow. Based on the assumption that management accounting develops as companies grow we problematize on the subject and how it affects SMEs in strong growth. Purpose: The purpose of the thesis is to increase and deepen the understanding of how management accounting develops in rapidly growing SMEs. The purpose is also to identify the critical events and factors that have taken place during the growth which have caused changes in their respecitive management accounting. Method: The study is of a qualitative nature in the form of a multiple-case study. The primary data collection has been made using semi-structured interviews. Interviews have been conducted with both leading people and employees to create an idea of how the management has developed over time in the two companies. Conclusion: What characterizes management accounting in the case companies is partly a series of critical events that has affected decisions and future development of the companies. In addition, both external and internal effects have contributed to the critical events and development of the companies. Finally, the companies have acted to adapt their management accounting as they have had rapid growth, a work that is consistently characterized by a gradual development to meet the needs that have arisen.

Undervisning med växande geometriska mönster ses som en bro mellan aritmetiskt och algebraiskt tänkande genom att elever möter uppgifter som möjliggör generalisering av aritmetiska uttryck. Eftersom svenska elever i internationella tester visar upp bättre resultat inom aritmetik än algebra är syftet med studien att ta reda på vilka aspekter som är kritiska för elevers utveckling från ett aritmetiskt till ett algebraiskt tänkande. Studien är en learning study där en lektion planerades och genomfördes i tre klasser i årskurs 5 och 6. Lektionerna kompletterades med ett för- och eftertest som tillsammans med lektionerna bidrog till studiens resultat. Vid planering och analys av lektionerna tillämpades variationsteorin som fokuserar på vilket lärande som möjliggörs, vilket lärande som sker och vad som kan förbättra lärande. Resultatet är att det kan vara kritiskt för elever att urskilja regelbundenheten i växande geometriska mönster och att särskilja regelbundenheten från proportionalitet. En ytterligare kritisk aspekt kan vara att urskilja bokstävers betydelse inom matematik. I resultatet framkommer även att två av de variationsmönster som använts kan möjliggöra urskiljning av aspekter som kan vara kritiska för elever. Vid undervisning med växande geometriska mönster finns det flera aspekter som lärare behöver möjliggöra för elever att urskilja. Genom att använda genomtänkta variationsmönster kan aspekterna synliggöras och därmed utveckla elevers förståelse för algebra. / Teaching of growing geometrical patterns should be seen as a bridge between arithmetic and algebraic thinking, that through giving the students tasks that enables generalization of arithmetic expressions. Swedish students’ results show that they perform better arithmetically than algebraically therefore, the aim of this study is to ascertain which aspects that are critical to students’ development from an arithmetic thinking to an algebraic thinking. This study is a learning study where a lesson was planned and performed in three different classes in grade 5 and 6. The lessons included a pre-test and a posttest to further validate the study and the tests, combined with the lessons, contributed to the result of the study. When planning and analyzing the lessons the theory that was applied was variation theory which focuses on what is learned, what learning that takes place and what can be improved to further the learning. The result of this study shows that it can be critical for students to discern regularities in growing geometrical patterns and also to separate regularity from proportionality. Furthermore, the study found another critical aspect which is to discern the meaning of letters within mathematics. The result also reveals two variation patterns which enables discerning of aspects that appear critical for students. When teaching about growing geometrical patterns there are several aspects teachers need to make possible for students to discern. Through utilization of variation patterns that are well prepared and thought through these aspects can be visualized and consequently auxiliary advance students understanding for algebra.

O século XIX testemunhou o aumento exponencial dos movimentos migratórios de europeus para as Américas, que se prolongaram até o início da Primeira Guerra Mundial, abrindo caminho para o negócio de recrutamento e transporte de braços. Este trabalho analisa o fluxo estabelecido entre dois lados do Atlântico, mais especificamente Itália e Brasil, discutindo fatores condicionantes, reflexos internos e externos, contradições e particularidades. Na Itália, o foco está direcionado para os interesses conflitantes dos grupos envolvidos: companhias de navegação, agentes e subagentes. Analisa-se a experiência imigratória no Brasil e, em especial, São Paulo, caracterizado pela política de subsídio. Se o financiamento promovido pelo governo paulista era essencial para a criação de um grande fluxo que atendesse a demanda da cafeicultura, por outro lado, constituiu-se em fonte de rendimentos para indivíduos e empresas ligadas à execução dos serviços de recrutamento e transporte. A análise dos balanços financeiros e relatórios dos conselhos administrativos das companhias de navegação italianas evidencia a importância da emigração como fonte consistente para sua saúde financeira. Ainda dentro das engrenagens responsáveis pelo fluxo, o enfoque recai sobre as agências de introdução de imigrantes que celebraram contratos com o governo paulista. Em suma, uma rede de negócios estabelecida nos dois lados do Atlântico, cujos objetivos eram claros: auferir lucros com a emigração. / The XIX century witnessed the exponential increase of the migratory movements of Europeans to Americas, which were extended up to the beggining of the First World War, opening way for the business of recruitment and transport of arms. This work analyses the flow established between two sides of the Atlantic, more specifically Italy and Brazil, discussing restrective factors, internal and external reflexes, contradictions and peculiarities. In Italy, the focus is on the conflicting interests of the involved groups: shipping companies, agents and subagents. The immigratory experience is analised in Brazil, and specially in São Paulo, characterized by the politics of subsidy. If the financing promoted by the government of São Paulo was essential for the creation of a big flow that was attending the demand of the coffee growing, on the other side it was constituted in source of profits for individuals and enterprises connected with the services of recruitment and transport. The analysis from the balances in cash and reports from the administrative councils from the Italian shipping companies makes clear the importance of emigration as a consistent source for her financial health. Still inside the gears responsible for the flow, the approach falls on the agencies of immigrants\' introduction that celebrated contracts with the government of São Paulo. Summing up, a business net established in two sides of the Atlantic, which objectives were clear: to derive profits with the emigration.

Essa pesquisa trata do crescimento urbano e implicações ambientais de uma área na bacia do Guarapiranga. Essa área abrange parte dos municípios de São Paulo, Itapecerica da Serra e Embu Guaçu. O intuito é apresentar a evolução urbana da área entre os anos 1962 e 2001, com análises de fotografias áreas. Em uma escala intra-urbana, a pesquisa apresenta a interpretação de fotografias áreas para o ano de 2001 criando a carta de áreas homogêneas urbanas. Os principais dados dessa pesquisa são para o ano de 2001, de modo que pudemos os indicadores sócio-demográficos dos dados do censo do IBGE realizado para o ano de 2000.Com todos esses dados, podemos apontar os vetores de crescimento urbano da área, assim como seus problemas sócio-ambientais. / This research is about the urban growing in an area wich is considered model in the Guarapiranga´s Basin. This area encloses the citys of São Paulo, Itapecerica da Serra and Embu Guaçu. The intention is present the urban evolution of the area between the years 1962 and 2001, with analyses of aerial photography. In intra urban scale, the research presents the aerial photography\'s urban interpretation for the year 2001, making the maps of the urban homogenous zones areas. The main data or this research is about the year 2001, in the way that we could have the social demography in the IBGE´s censo data for the year 2000. With all this data, we have got the growing expansion city vectors as also the social and ambient problems.

Diversos atletas e praticantes de atividades físicas incorporam em suas rotinas de treinamento, exercícios aeróbios e de força motora simultaneamente. Contudo, essa combinação conhecida como treinamento concorrente (TC) tem demonstrado uma atenuação da resposta adaptativa da força e hipertrofia muscular. O presente estudo analisou se alguns genes e proteínas envolvidos na resposta hipertrófica e na biogênse mitocondrial do músculo esquelético poderiam explicar a atenuação da resposta adaptativa com o TC. Trinta e sete sujeitos foram divididos nos grupos: controle (C), aeróbio (TA), força (TF) e concorrente (TC) e submetidos a oito semanas de treinamento. Os resultados significantes foram: aumento na força dinâmica máxima de 270,3 (±45,5) para 320,3 (±57,0) Kg para o TF e de 268,4 (±47,6) para 315,7 (±63,5) para o TC; área de secção transversa do quadríceps de 8332,4 (±817,5) mm2 para 8849,5 (±893,3) mm2 para o TF e de 8340,8 (±1000,0) mm2 para 8996,8 (±919,5 )mm2 para o TC; o gene da mTOR demonstrou aumento significante de 1,01 (±0,10) U.A para 1,44 (±0,17) U.A no TF e redução de 1,01 (±0,15) para 0,536 (± 0,25) U.A da p70S6K1 no TC; a expressão total da proteína p70S6K1 demonstrou aumentou no grupo TC em relação ao C (1,1 (±0,2) U.A vs 0,8 (±0,3) U.A), a fosforilação da Akt no resíduo ser473 e da p70S6K1no resíduo thr389 aumentou somente no TF em relação ao C (1,3 (±0,2) U.A vs 0,9 (±0,1) U.A e 1,3 (±0,4) vs 0,8 (±0,3) U.A, respectivamente). O grupo TF e TC demonstraram adaptações similares nas variáveis de força e hipertrofia muscular apesar de algumas diferenças na resposta molecular. Esses achados indicam que na fase inicial do TC as diferenças na adaptação molecular não refletem em alterações na força e hipertrofia muscular quando comparadas ao TF / Many athletes and individuals involved in physical training perform strength and endurance exercises in the same training unit. However, this combination, referred as concurrent training (CT), has shown to blunt strength and skeletal muscle growth responses. This study investigated whether some genes and proteins associated with muscle growth and mitochondrial biogenesis may explain the decreased adaptive response to CT. Thirty seven participants were divided into four groups: control (C), endurance (TA), strength (TF) and concurrent (TC) and submitted to eight weeks of training. Significant results were found in the following variables from pre to post training: maximum dynamic strength - TF from 270,3 (±45,5) to 320,3 (±57,0) Kg and TC from 268,4 (±47,6) to 315,7 (±63,5); quadriceps cross sectional area (CSA) - TF from 8332,4 (±817,5) mm2 to 8849,5 (±893,3) mm2, TC from 8340,8 (±1000,0) mm2 to 8996,8 (±919,5)mm2; mTOR gene expression increased significantly post-training only for the TF (1,01 (±0,10) A.U to 1,44 (±0,17) A.U) and p70S6K1 was significantly reduced post-training (1,01 (±0,15) to 0,536 (± 0,25) A.U) for the TC; p70S6K1 total protein content was significantly greater after TC when compared with C (1,1 (±0,2) U.A vs 0,8 (±0,3)) and phosphorylation of both Akt at ser473 and p70S6K1 at thr389 increased only after TF compared with C (1,3 (±0,2) U.A vs 0,9 (±0,1) U.A and 1,3 (±0,4) vs 0,8 (±0,3) U.A, respectively). TF and TC groups had similar improvements in muscle strength and hypertrophy, besides some differences in the molecular responses. These differences at the molecular level in early phases of the TC do not blunt muscle strength and hypertrophy adaptations compared with the TF

The aim of this thesis is to evaluate basic yield formation and food quality of grain in selected winter wheat varieties, depending on the intensity of cultivation. There was 5 varieties chosen for the experiment of which 3 was linear (Patras, Turandot and Rumor) and 2 varieties was hybrid (Hyfi and Hybery). Each variety was repeated three times during the experiment. The vegetation was assessed in two variations - the checking one and the one fertilized by the nitrogen. This experiment was conducted in growing season 2017/2018 on the field of Faculty of Agriculture of University of South Bohemia. The withdrawal of the vegetal material for the purpose of the following sample analysis was done right before the harvesting. The yield formation and the health condition of the vegetation was assessed in the fieldwork. The determination of qualitative parameters was made with the help of the apparatus in the laboratory of the faculty. Then, the results was marked in tables and graphics. Finally, the statistical evaluation of the obtained data. An average yield of all assessed varieties of the winter wheat was 8,52 t.ha-1 at the checking variation. On the contrary, at the nitrogen fertilized one the average yield was 8,94 t.ha-1. The evaluated varieties in the growing season 2017/2018 had the baking quality A and B. Regarding the qualitative parameters, there was found lower values of volume quantity than those that are stated in the norm for the food wheat (at least 76,0 kg.hl-1). The highest values were found at the variety Turandot fertilized by nitrogen (77,8 kg.hl-1), which met the requirements of the ČSN 4611 00-2.

Cianobactérias, conhecidas por sua habilidade de sintetizar metabólitos com ação tóxica, podem se tornar dominantes em águas com altas concentrações de nitrogênio e fósforo. Embora a toxicidade do glifosato, o herbicida mais usado no mundo, em alguns organismos aquáticos seja conhecida, poucos estudos abordam o efeito desse composto sobre a produção de metabólitos secundários por cianobactérias. O objetivo deste trabalho foi avaliar a influência de diferentes concentrações de glifosato (produto técnico) sobre o crescimento e produção de cianotoxinas e microgininas pelas cepas brasileiras Microcystis aeruginosa LTPNA 08 e Cylindrospermopsis raciborskii CENA 302. Na presença de 15 mg/L de glifosato, o crescimento e a produção de toxinas pela M. aeruginosa foram reduzidos e de microgininas significativamente aumentada. Já a C. raciborskii, quando exposta à 20 mg/L de glifosato teve seu crescimento e síntese de clorofila-a, carotenoides e saxitoxinas aumentados. Concentrações superiores a 20 e 30 mg/L impediram o crescimento celular das cepas LTPNA 08 e CENA 302, respectivamente. A análise de ácidos graxos mostrou perfis bastante distintos entre as cepas. Na cepa LTPNA 08, enquanto que na presença de 10 mg/L de glifosato ocorreu diminuição do teor do ácido linoleico, o ácido estearidônico foi aumentado. Nenhuma das concentrações testadas promoveu alteração sobre o perfil de ácidos graxos da cepa CENA 302. A toxicidade de 5 produtos formulados a base de glifosato foi comparada ao produto técnico em ambas as linhagens-teste. Observou-se uma resistência distinta entre as cepas e toxicidade também variável entre as formulações comerciais. Sendo assim, diante da elevada resistência das cianobactérias M. aeruginosa e C. raciborskii ao glifosato, e considerando-se a elevada interferência antrópica através das práticas agrícolas, pode-se inferir que o uso excessivo e frequente desse herbicida é capaz de estimular o crescimento e dominância desses organismos, podendo modificar a estrutura e funcionalidade de ecossistemas aquáticos / Cyanobacteria, known for their ability to synthesize toxic metabolites, can become dominant in water bodies with high concentrations of nitrogen and phosphorus. Although the toxicity of glyphosate, the most widely used herbicide in the world, in some aquatic organisms is well known, few studies address the effect of this compound on the production of secondary metabolites by cyanobacteria. The aim of this study was to evaluate the influence of different concentrations the herbicide glyphosate (technical grade) on growth and production of cyanotoxins and microginins by Brazilian strains of Microcystis aeruginosa LTPNA 08 and Cylindrospermopsis raciborskii CENA 302. In the presence of 15 mg/L of glyphosate, growth and toxin production by M. aeruginosa were reduced and microginins cell quota significantly increased. The C. raciborskii strain, when exposed to 20 mg/L of glyphosate, had the growth, and chlorophyll-a, carotenoids and saxitoxins production increased. Concentrations above 20 and 30 mg/L prevented cell growth of LTPNA 08 and CENA 302 strains, respectively. Fatty acid analysis showed distinct profiles among the strains. When exposed to 10 mg/L of glyphosate, a decrease in the linoleic acid and increase in stearidonic acid content were observed in M. aeruginosa LTPNA 08 strain. None of the tested concentrations of glyphosate promoted change on the fatty acid profile of CENA 302 strain. The toxicity of 5 glyphosate formulated products was compared to technical product to both strains. There was a distinct resistance among strains and also a variable toxicity among formulated products. Thus, given the high glyphosate resistance of M. aeruginosa and C. raciborskii cyanobacteria, and considering the high anthropogenic interference through agri cultural practices, it can be inferred that excessive and frequent use of this herbicide is able to stimulate growth and dominance of these organisms, which may modify the structure and function of aquatic ecosystems

by Ng Ki Chi. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 130-145). / Acknowledgments / Abstract / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Waste Management in Hong Kong --- p.1 / Chapter 1.2 --- Sources of Organic Wastes and Their Characteristics --- p.4 / Chapter 1.2.1 --- Animal manure --- p.5 / Chapter 1.2.2 --- Sewage sludge --- p.7 / Chapter 1.2.3 --- Food waste --- p.7 / Chapter 1.3 --- Organic Waste Recycling --- p.9 / Chapter 1.3.1 --- Organic wastes for land application --- p.9 / Chapter 1.3.1.1 --- Animal manure --- p.11 / Chapter 1.3.1.2 --- Sewage sludge --- p.12 / Chapter 1.3.1.3 --- Plant residue --- p.14 / Chapter 1.3.2 --- Composting --- p.15 / Chapter 1.3.3 --- Biogas production --- p.16 / Chapter 1.3.4 --- Organic wastes as feedstuffs or feed supplements --- p.18 / Chapter 1.3.4.1 --- Animal manure --- p.18 / Chapter 1.3.4.2 --- Sewage sludge --- p.19 / Chapter 1.3.4.3 --- Plant residue --- p.20 / Chapter 1.3.4.4 --- Biological products from waste conversion --- p.21 / Chapter 1.4 --- Objectives and Outlines of the Present Study --- p.26 / Chapter Chapter 2 --- Vermiculture of Eisenia foetida and Pheretima asciatica on Different Agro- industrial Wastes --- p.29 / Chapter 2.1 --- Introduction --- p.29 / Chapter 2.2 --- Materials and Methods --- p.34 / Chapter 2.2.1 --- Collection of materials --- p.34 / Chapter 2.2.2 --- Preparation of earthworms --- p.34 / Chapter 2.2.3 --- Culture trial --- p.35 / Chapter 2.2.4 --- Harvesting of earthworms --- p.35 / Chapter 2.2.5 --- "Chemical analysis of bedding material, wastes and worm tissues" --- p.37 / Chapter 2.2.6 --- Statistical analysis --- p.38 / Chapter 2.3 --- Results and Discussion --- p.38 / Chapter 2.3.1 --- Chemical properties of organic wastes and bedding material --- p.38 / Chapter 2.3.2 --- Heavy metal contents in bedding material and organic wastes --- p.41 / Chapter 2.3.3 --- Effects of organic wastes on worm growth --- p.43 / Chapter 2.3.4 --- Effects of organic wastes on protein contents and protein yields --- p.47 / Chapter 2.4 --- Conclusions --- p.49 / Chapter Chapter 3 --- "Effects of Soybean Waste, Pig Manure and Digested Sludge on the Quality of Worm Meal From Eisenia foetida and Pheretima asciatica" --- p.53 / Chapter 3.1 --- Introduction --- p.53 / Chapter 3.2 --- Materials and Methods --- p.59 / Chapter 3.2.1 --- Collection of materials --- p.59 / Chapter 3.2.2 --- Preparation of earthworms --- p.59 / Chapter 3.2.3 --- Experimental design and setup --- p.59 / Chapter 3.2.4 --- Harvesting of earthworms --- p.61 / Chapter 3.2.5 --- Chemical analysis of worm tissues --- p.61 / Chapter 3.2.6 --- Statistical analysis --- p.63 / Chapter 3.3 --- Results and Discussion --- p.63 / Chapter 3.3.1 --- Effects of wastes on worm growth --- p.63 / Chapter 3.3.2 --- Nutrient contents of earthworms from different wastes --- p.69 / Chapter 3.3.3 --- Heavy metal contents in earthworm tissues --- p.72 / Chapter 3.4 --- Conclusions --- p.81 / Chapter Chapter 4 --- Worm Meal as Protein Source for Fish Diet --- p.82 / Chapter 4.1 --- Introduction --- p.82 / Chapter 4.2 --- Materials and Methods --- p.85 / Chapter 4.2.1 --- Preparation of experimental diets --- p.85 / Chapter 4.2.2 --- Feeding experiment --- p.88 / Chapter 4.2.3 --- Chemical analysis --- p.90 / Chapter 4.2.4 --- Statistical analysis --- p.90 / Chapter 4.3 --- Results and Discussion --- p.90 / Chapter 4.3.1 --- Growth response --- p.90 / Chapter 4.3.1.1 --- Growth --- p.91 / Chapter 4.3.1.2 --- Mortality --- p.95 / Chapter 4.3.2 --- Tissue chemical composition --- p.95 / Chapter 4.3.2.1 --- Nutritional contents --- p.97 / Chapter 4.3.2.2 --- Heavy metal contents --- p.97 / Chapter 4.4 --- Conclusions --- p.99 / Chapter Chapter 5 --- Worm Worked Bedding as Potting Media for Plant Growth --- p.103 / Chapter 5.1 --- Introduction --- p.103 / Chapter 5.2 --- Materials and Methods --- p.106 / Chapter 5.2.1 --- Preparation of potting media --- p.106 / Chapter 5.2.2 --- Chemical analysis of potting media --- p.106 / Chapter 5.2.3 --- Plant growth experiment --- p.107 / Chapter 5.2.4 --- Tissue analysis --- p.109 / Chapter 5.2.5 --- Statistical analysis --- p.109 / Chapter 5.3 --- Results and Discussion --- p.109 / Chapter 5.3.1 --- Chemical properties of potting media --- p.109 / Chapter 5.3.2 --- Plant growth status --- p.114 / Chapter 5.3.3 --- Heavy metal contents in plant tissues --- p.119 / Chapter 5.4 --- Conclusions --- p.125 / Chapter Chapter 6 --- General Conclusions --- p.126 / References --- p.130

Cai Yi Jin. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 118-128). / Chapter 1. --- Introduction / Chapter 1.1 --- Importance and Cultivation history of edible mushroom --- p.1 / Chapter 1.2 --- Variety and structure of growth substrates for mushroom --- p.4 / Chapter 1.3 --- Mushroom growth and substrate-degrading enzymes --- p.8 / Chapter 1.4 --- Purpose of study --- p.15 / Chapter 2. --- Methods and Materials / Chapter 2.1 --- Organisms --- p.17 / Chapter 2.2 --- Media --- p.17 / Chapter 2.3 --- Culture conditions --- p.21 / Chapter 2.3.1 --- Growth temperature --- p.21 / Chapter 2.3.2 --- Growth Studies --- p.21 / Chapter 2.3.2.1 --- Effect of pH on mycelial growth --- p.21 / Chapter 2.3.2.2 --- Effect of different carbon sources on mycelial growth --- p.21 / Chapter 2.3.2.3 --- Effect of lignin-related phenolic monomers and tannin derivatives on fungal growth --- p.22 / Chapter 2.3.3 --- Culture conditions for production of extracellular enzymes --- p.23 / Chapter 2.3.3.1 --- Tyrosinase --- p.23 / Chapter 2.3.3.2 --- Laccase --- p.23 / Chapter 2.3.3.3 --- Manganese-dependent Peroxidase and Lignin Peroxidase --- p.23 / Chapter 2.3.3.4 --- Cellulytic and Xylanolytic enzymes --- p.24 / Chapter 2.3.3.5 --- Lipase --- p.25 / Chapter 2.3.4 --- Culture conditions for studying properties of cellulases of V. volvacea --- p.26 / Chapter 2.3.4.1 --- CMCase --- p.26 / Chapter 2.3.4.2 --- "CMCase, FPase and β-Glucosidase" --- p.26 / Chapter 2.3.4.3 --- β-Glucosidase --- p.26 / Chapter 2.4 --- Enzyme assay --- p.27 / Chapter 2.4.1 --- Tyrosinase --- p.27 / Chapter 2.4.2 --- Laccase --- p.27 / Chapter a. --- o-Tolidine Method --- p.27 / Chapter b. --- ABTS Method --- p.28 / Chapter c. --- Syringaldazine Method --- p.28 / Chapter 2.4.3 --- Lignin peroxidase --- p.29 / Chapter 2.4.4 --- Manganese-dependent peroxidase --- p.29 / Chapter 2.4.5 --- Exoglucanase (avicelase) --- p.30 / Chapter 2.4.6 --- Endoglucanase (carboxymethylcellulase or CMCase) --- p.31 / Chapter 2.4.7 --- Filter paper digesting enzyme (FPase) --- p.32 / Chapter 2.4.8 --- P-Glucosidase --- p.32 / Chapter 2.4.9 --- Xylanase --- p.34 / Chapter 2.4.10 --- β-Xylosidase --- p.34 / Chapter 2.4.11 --- Lipase --- p.36 / Chapter 2.5 --- Other analytical methods --- p.36 / Chapter 2.5.1 --- Determination of phenol oxidase activity by the Bavendamm reaction --- p.36 / Chapter 2.5.2 --- Qualitative evaluation of CMCase by Congo red staining --- p.37 / Chapter 2.5.3 --- Effect of phenolic monomers and tannic acid on CMCase activity of V. volvacea --- p.38 / Chapter 2.5.4 --- Protein determination --- p.39 / Chapter 2.5.5 --- Non-denaturing gel electrophoresis pattern of fungal laccases --- p.39 / Chapter 2.6 --- Chemicals --- p.39 / Chapter 3. --- Results / Chapter 3.1 --- Growth and Nutritional characteristics --- p.44 / Chapter 3.1.1 --- Fungal growth on defined and non-defined culture media --- p.44 / Chapter 3.1.2 --- Effect of carbon source on fungal --- p.45 / Chapter 3.1.3 --- Effect of pH on fungal growth --- p.45 / Chapter 3.2 --- Effect of lignin-related phenolic monomers and tannin derivatives on fungal growth --- p.45 / Chapter 3.2.1 --- Effect of lignin-related phenolic monomers on fungal growth --- p.45 / Chapter 3.2.2 --- Effect of tannin derivatives on fungal growth --- p.61 / Chapter 3.3 --- Phenol Oxidase --- p.67 / Chapter 3.3.1 --- Phenol oxidase --- p.67 / Chapter 3.3.1.1 --- Guaiacol-reacting enzyme --- p.67 / Chapter 3.3.1.2 --- o-Anisidine oxidizing enzyme --- p.68 / Chapter 3.3.2 --- Tyrosinase --- p.69 / Chapter 3.3.3 --- Laccase --- p.69 / Chapter 3.3.3.1 --- "Laccase detected by o-Tolidine, ABTS Syringaldazine" --- p.69 / Chapter 3.3.3.2 --- Effect of pH on laccase activity --- p.69 / Chapter 3.4 --- Lignin-Transforming Enzymes --- p.73 / Chapter 3.4.1 --- Lignin peroxidase (LP) --- p.73 / Chapter 3.4.2 --- Manganese-dependent peroxidase (MnP) --- p.74 / Chapter 3.5 --- Cellulases --- p.78 / Chapter 3.5.1. --- Cellulases of V. volvacea --- p.78 / Chapter 3.5.1.1 --- Qualitative estimation of cellulose-degrading enzymes of V. volvacea grown on different substrates --- p.78 / Chapter 3.5.1.2 --- Influence of pH and temperature --- p.79 / Chapter 3.5.1.3 --- Cellulolytic activities in cultures grown on cellulose --- p.83 / Chapter 3.5.1.4 --- Cellulolytic activities in cultures grown on paddy straw --- p.91 / Chapter 3.5.1.5 --- β-Glucosidase activity in cultures grown on cellobiose --- p.91 / Chapter 3.5.1.6 --- Effect of lignin-related phenolic monomers and tannic acid on CMCase of V. volvacea --- p.95 / Chapter 3.5.2 --- Cellulases of P.sajor-caju --- p.96 / Chapter 3.5.3 --- Cellulases of L. edodes --- p.96 / Chapter 3.6 --- Xylanase --- p.96 / Chapter 3.6.1 --- "Xylanase of V. volvacea, strain V34" --- p.96 / Chapter 3.6.2 --- Xylanase of P.sajor-caju --- p.100 / Chapter 3.6.3 --- Xylanase of L. edodes --- p.100 / Chapter 3.7 --- Lipase of V. volvacea --- p.103 / Chapter 4. --- Discussion / Chapter 4.1. --- Carbon nutrition and pH for fungal growth --- p.104 / Chapter 4.1.1 --- Carbon nutrition --- p.104 / Chapter 4.1.2 --- pH --- p.104 / Chapter 4.2 --- "Effect of lignin-related phenolic monomers and tannin derivatives on fungal growth of L. edodes, P. sajor-caju and V, volvacea" --- p.105 / Chapter 4.2.1 --- Lignin-related phenolic monomers --- p.105 / Chapter 4.2.2 --- Tannin derivatives --- p.107 / Chapter 4.3 --- "Production of phenoloxidases by V. volvacea, L. edodes and P. sajor-caju" --- p.108 / Chapter 4.3.1 --- Guaiacol- and Anisidine reacting enzymes and Tyrosinase --- p.108 / Chapter 4.3.2 --- Laccase --- p.109 / Chapter 4.4. --- "Lignin-degrading Enzymes of V. volvacea, P. sajor-caju and L. edodes" --- p.110 / Chapter 4.5. --- "Cellulolytic and Hemicellulolytic Activity of V. volvacea, P.sajor-caju and L. edodes" --- p.113 / References --- p.118 / Appendix1 --- p.129

Previous work has shown that despite the lack of oxygen limitation, transformed roots of Artemisia annua had lower biomass productivity in a nutrient mist bioreactor than in a liquid-phase bubble column reactor where the roots demonstrated metabolic signs of oxygen stress. Mathematical modeling suggested that the roots were too sparsely packed to capture mist particles efficiently and to achieve high growth rates. In this study, higher packing fractions were tested, and the growth rate increased significantly. Similarly, higher sucrose concentrations increased the growth rate. Growth kinetics for 2, 4, and 6 days showed an unexpected decrease or stationary growth rate after only 4 days for both 3% and 5% sucrose feeds. Residual media analyses indicated that carbon was not exhausted, nor were other major nutrients including phosphate. Increasing the misting frequency such that the total amount of carbon delivered from a 3% sucrose feed was equivalent to that delivered in a 5% sucrose feed showed that growth was affected by the modified cycle. These studies showed that both the concentration of carbon source and alteration of misting frequency can significantly increase growth rates of hairy roots in mist reactors.