Lake Moomaw, a 1,024-ha flood control reservoir in Bath and Allegheny counties, Virginia contains a migratory population of smallmouth bass that congregate in the headwaters of the reservoir during the spring spawning period, where they are vulnerable to a shore-based, harvest-oriented fishery. The extent of this fishery and resulting effects on the small mouth bass population were analyzed by means of a creel survey in the headwaters area during the spring spawning seasons of 1995 and 1996. Effort, catch, and harvest, as well as user characteristics and motivations data were obtained from direct interviews with anglers using this area. Estimates for 1995 indicated extensive fishing pressure per ha, with 1,167 angler hours per ha spent fishing for smallmouth bass in the headwaters, while in 1996 almost 1,400 angler hours per ha were spent in this area. Catch and harvest rates were relatively low and sustainable during both years, with 124 small mouth bass caught and 82 harvested in 1995, while 318 small mouth bass were caught and 222 harvested in 1996. An extensive capture-recapture study yielded estimates of exploitation rates for small mouth bass in the reservoir of 12- 15% annually. Exploitation of the whole-lake population occurring in the spring headwaters fishery was estimated at 4-6%, while the exploitation rate on the subset of the population using the headwaters during the spring was 11-14%. Analysis of movements of snlallmouth bass in the reservoir using ultrasonic telemetry and dart tag recaptures indicated that the subset of the population using the headwaters was mainly drawn from the upper and middle portions of the reservoir, and that significant amounts of spawning occurred in the lower section of the reservoir as well. Areas used by smallmouth bass for reproduction were documented with summer and fall electrofishing to determine relative abundance of young-of-the-year smallmouth bass in the reservoir, and showed spawning to take place throughout the reservoir. Densities of young-of-the-year shifted as fall progressed, with highest densities in the middle portion of the reservoir in early fall, indicating that reproductive inputs from the headwaters were realized in the lake as fall progressed. The headwaters fishery is a high-profile activity which, during 1995-96, had a low and sustainable impact on the Lake Moomaw smallmouth bass population. / Master of Science

Thesis advisor: Noah P. Snyder / Nitrogen is an important component in the biogeochemical processes of freshwater systems. Likewise, it is unknown if, and to what magnitude, changes in land use in the watersheds of New England lakes have affected nitrogen availability. This study examines the effects of land-use change on the present and historic isotopic signatures of nitrogen in three New England lakes of varied histories, Lower South Branch Pond, Little Kennebago Lake, and Sennebec Pond. The histories of all three sites indicate minimal discernible disturbance before the onset of Euro-American-induced land use change. For two sites, the dominant mechanism of change was timber harvest, which began in the latter half of the 19th century. Sediment cores for each site were examined and variations in geochemical and sedimentological indicators were evaluated in the context of changes within respective basins. Statistical analysis indicates significant shifts in the means and variance of the geochemistry within the Little Kennebago Lake and Sennebec Pond watersheds after the incursion of Euro-American settlers, while the Lower South Branch Pond watershed displays similarities to a more widespread signal of anthropogenic nitrogen that has been deposited remotely. The record of magnetic susceptibility in Little Kennebago Lake displays the largest variation compared to the other two lakes, which may indicate that the magnitude of land-use change within the basin was more impactful relative to Lower South Branch Pond and Sennebec Pond. This is significant in that all three sites experienced some level of land-clearance. / Thesis (MS) — Boston College, 2023. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: Earth and Environmental Sciences.

In malting barley, rapid germination is desirable and linked to end use quality. Modern malting varieties have been bred for low seed dormancy leading to issues with pre-harvest sprouting in wetter growing regions. To maintain malting capacity while minimizing germination on the maternal plant requires in-depth understanding of the genetic regulation of dormancy in malting barley. Currently, the major effect QTLs SD1 and SD2 have been shown to influence dormancy across multiple populations of barley, though the physiological mechanisms involved remain unclear. To search for novel genetic regions that influence primary dormancy, three mapping populations were assessed including two Canadian biparental populations (Synch and Legci) as well as a diversity panel sourced from multiple locations worldwide (ICARDA AM-14). The SD2 locus had a major effect in the Synch population while the SD1 locus had a major effect in the Legci population and neither SD1 nor SD2 were linked to dormancy in the diversity panel. Instead, 14 additional marker trait associations were identified in AM-14 suggesting that investigating a broader range of genetic regulation of dormancy outside of North American varieties may provide solutions to regulate this trait. Additional testing on SD1 revealed that variation at this locus did not affect ABA sensitivity during germination or GA or ABA-regulated gene expression during grain fill. Indeed, lines containing the non-dormant SD1 allele germinate at a similar rate as the dormant SD1 seeds when the glumella was removed from the embryo. This indicated that the effect of the alanine aminotransferase gene underlying the SD1 allele is dependent on physical restriction on the embryo or the hypoxic effects produced by the glumella. Imposing a hypoxic (5% oxygen) environment on exposed embryos revealed an association between non-dormancy at SD1 and reduced sensitivity to the suppressive effects of hypoxia on germination. This suggests that alanine aminotransferase regulates dormancy release during barley germination at least in part through regulation of the seed’s response to hypoxia.

This research involved testing several aspects of industrial hemp (Cannabis sativa L.) production, including the impact of tillage on seed and fiber production, optimal harvest time for seed yield and quality, the response of seed yield to nitrogen fertility rates, and the potential of hemp as a forage crop. A three-year study was conducted in Blacksburg and Orange of Virginia State to assess the effects of tillage management and production systems (e.g., seed, dual, and fiber) on hemp establishment and productivity. Two cultivars, Joey (a dual-purpose variety) and EcoFibre (bred specifically for fiber), were planted into seedbeds prepared with conventional tillage and no-till management. The cultivar Joey, lower plant populations under seed production systems resulted in taller plants (P = 0.0002) compared to the dual-purpose production systems in 2020. Greater plant heights (P < 0.0001) with fiber production systems in 2021 and 2022 were due to differences between cultivars and their time of flowering. Conventional tillage resulted in greater (P ≤ 0.0161) plant populations than no-tillage for all production systems in each year, and this response was more pronounced with fiber management in 2020 (tillage × production systems interaction; P = 0.0007). Greater (P < 0.001) yields with fiber systems observed in 2021 and 2022 were largely driven by the more productive EcoFibre cultivar. Despite treatment differences in population density, biomass and seed yields varied less by tillage management and production systems. Lower plant population density was associated with greater biomass and seed yields per plant. However, for desired fiber quality and mechanical harvest feasibility, a higher plant population density is recommended. A second study aimed to determine the optimum harvest time for seed yield of two hemp cultivars. 'Joey', and 'Grandi,', were established in Blacksburg and Orange, Virginia in mid-May/early June of 2021 and 2022. The experiment was conducted as a randomized complete block design with a repeated measurement arrangement and four replicates. Plants were harvested four times at one-week intervals starting in mid-summer. Harvest date significantly affected seed yield, with the response differing by cultivar (cultivar × date interaction; P = 0.001) in 2022 at the Orange site. In Blacksburg, seed yields were similar for the two cultivars and greatest at the second harvest each season (July 22, 2021, and July 25, 2022), although they were substantially lower in 2022 due to drought (1750 vs. 480 kg ha-1; P < 0.0001). In Orange, in 2021, as planting occurred late, harvests were also deferred until August 17, and seed yields were greatest at this first harvest (1180 kg ha-1; P<0.0001). In 2022, yields at the Orange location were highest for Grandi at the first harvest (July 21; 1510 kg ha-1) and for Joey at the second harvest (July 28; 1280 kg ha-1) (Harvest Time by Cultivar interaction, P = 0.0010). Over the subsequent weeks of harvest, yields drastically declined (16 to 41% in 2021 and 27 to 47% in 2022 in Blacksburg; 52% to 91% in 2021 and 28% to 65% in 2022 in Orange, compared to the highest yield). Harvest timing is critical to achieving optimum seed yield, and it varies with cultivar, eco-physiographic location, and weather (e.g., rainfall). Fatty acids (FA) varied by cultivar, location, and harvest timing, but patterns of response were not consistent across FA. Gamma-linolenic (P ≤ 0.002) and oleic acids (P ≤ 0.023) were generally greater in Joey, with greater arachidic acid (P ≤ 0.013) concentrations in Grandi. Stearidonic acid concentrations declined with later harvest date in Orange location (P ≤ 0.0034). A third study aimed to measure hemp's response to different N rates and to determine the ability to predict plant N content and seed yield based on UAV-based multispectral imagery. Two hemp cultivars, 'Joey' and 'Grandi', were planted and five N rates (0, 60, 120, 180, 240 kg N ha-1) were tested in Blacksburg, Virginia in 2020, 2021, 2022. Aerial image acquisition occurred at three different growth stages in 2021 using dji M 300 drones mounted with multispectral sensors. Red/Blue index (R2=0.89), near-infrared (NIR) band (R2=0.84) and Enhanced vegetation index (EVI) (R2=0.81) were better predictors of N content in leaf samples than other vegetation indices that were evaluated. Green normalized difference vegetation index (GNDVI) was the better predictor of hemp seed yield (R2=0.58) than other evaluated vegetation indices. The seed yield of hemp was influenced (P ≤ 0.0177) by the N input in all three experimental years. In 2020, seed yield did not increase steadily with the increase of N rate; the highest seed yield, 1640 kg ha-1, was observed at 120 kg N ha-1. In 2021, maximum seed yield of 2500 kg ha-1 occurred at the maximum N rate (240 kg N ha-1). In 2022, a weak response to N rate was observed; maximum seed yield was 380 kg ha-1, again at 240 kg N ha-1. The overall growth of the hemp plants was affected by limited rainfall and weed pressures in 2022, leading to a significant reduction in seed yield. Response to N rate will vary depending on other factors such as available soil moisture during the growing season, weed pressure, and growing period. A fourth study examined the yield and nutritive value of three hemp cultivars, 'Grandi', 'Joey', and 'EcoFibre' as potential forage crops when harvested at weekly intervals in Blacksburg, VA. The greatest biomass and TDN yields across cultivars were 3.17 Mg ha-1 and 2.08 Mg ha-1 respectively, at two months after establishment in 2021. In the dry 2022 season, biomass and TDN yield were 1.9 Mg ha-1 and 1.03 Mg ha 1, respectively, two months after establishment. Hemp nutritive value measures varied by cultivar and harvest time (P < 0.05). Depending on the cultivar and harvest time, hemp plant biomass contained 13 to 32% CP, 22 to 45% NDF, 20 to 38% ADF, 4 to 9% lignin, and 52 to 80% TDN (cultivar × time interaction; P < 0.05). Hemp CP and TDN decreased gradually with maturation while ADF, NDF, and lignin increased (P<0.0001); however, this decline with maturity did not appear as severe as occurs with many other forages. These preliminary results suggest that hemp has the potential to be used as a forage crop. More research is needed to address hemp management and utilization, including field establishment and production, harvest timing for optimum tonnage and forage quality, and animal intake and performance studies. These findings provide new insights into industrial hemp production in the mid-Atlantic region of the United States. Optimal tillage practices, precise harvest timing, appropriate N fertility rates, and proper management techniques all are crucial for maximizing hemp seed and fiber production and quality. Furthermore, hemp shows promise as a forage crop with its adaptability and favorable nutritional properties. Further research is warranted to refine cultivation techniques, improve crop quality, and explore the full potential of hemp in various industries. / Doctor of Philosophy / Industrial hemp (Cannabis sativa L.) is a versatile crop with numerous applications in various industries, but much work must be done to understand crop responses to management practices and improve its potential as a crop for greater sustainability. In this study, we explored different aspects of hemp agronomic management. Hemp traditionally has been planted into tilled fields, which increases the chance for soil erosion. We examined whether hemp could be established without tillage and found that although tilled fields generally had great populations of taller plants; total biomass and seed yields were not as influenced by tillage. Our research suggests that with some tweaking, hemp can be successfully established without soil tillage. Next, we investigated the optimal time to harvest hemp for maximum seed yield. Harvesting at the right moment is crucial, as seeds ripen unevenly, resulting in varying quality and yield. By carefully timing the harvest, we can maximize seed yield and ensure high-quality seeds. Our cultivars were best harvested in a late July to early August time frame. Under favorable weather conditions, we observed seed yields ranging from 1,180 to 2,510 kilograms per hectare, depending on the hemp cultivar and location. Additionally, we studied the response of hemp seed yield to nitrogen fertilization rates. Nitrogen is an essential nutrient for plant growth, and we found that nitrogen significantly influenced seed yield, although the pattern of response varied by growing conditions. Over three years, seed yields ranged from 380 to 2,510 kilograms per hectare. Yields generally increased with nitrogen inputs but were highly affected by available moisture. Fertility studies help farmers determine the ideal nitrogen levels for their hemp crops, promoting healthy growth, maximizing yield, and minimizing environmental contamination. Within this study, we also evaluated aerial imagery technologies to monitor plant nitrogen status and we observed that remote sensing technologies are promising for building predictive nutrient management tools. Lastly, we explored the potential of hemp as a forage crop. Hemp plants have unique nutritional properties (e.g., protein, fatty acids) and can be used as feed for livestock. We investigated the best time to harvest hemp for maximum biomass and nutrient content, important factors for animal nutrition. Hemp plants grow rapidly and within two months after establishment they yielded up to 3.17 metric tons of biomass per hectare, with relatively high nutritional value. Overall, these studies provide valuable insights into hemp production, including the importance of tillage practices, optimal harvest timing, and appropriate nutrient management. By applying these findings, farmers can enhance their hemp cultivation techniques, resulting in higher yields, improved crop quality, and better environmental outcomes.

Understanding forest carbon uptake and associated growth response is important for carbon sequestration and water management practices given the large quantities of carbon stored in forest ecosystems. Climate variability and forest management practices influence the magnitude and rate of soil CO2 efflux; however, their combined effects are complex and not well understood. This study investigated the response of soil CO2 efflux to the combined effects of climate variability, including those caused by climate oscillations, and shelterwood harvesting in a mature temperate white pine (Pinus strobes L.) forest, located near Lake Erie in southern Ontario, Canada. Analyses indicated that local winter temperatures and precipitation were influenced by climate oscillations, which affected forest carbon dynamics. After the shelterwood harvest removed approximately a third of the overstory canopy, no significant differences were found for soil temperature and soil moisture between the pre-harvesting (2008 to 2011) and post-harvesting (2012 to 2014) periods. Despite similar climate conditions pre- and post-harvesting, soil CO2 effluxes post-harvesting were lower. A Gaussian-Gamma specification model determined that heterotrophic (autotrophic) respiration decreased (increased) between pre- and post-harvesting, respectively. Mineral-soil respiration were similar pre- and post-harvesting. Soil CO2 efflux accounted for 78±9% of the annual ecosystem respiration (RE), derived using eddy-covariance fluxes. However, the overall net ecosystem productivity showed no significant difference between pre- and post-harvesting. This was attributed to an increase in the gross ecosystem productivity post-harvesting, compensating for the increased losses (i.e. increased RE). This study highlights the complexities of measuring various components of ecosystem respiration after a disturbance, such as a harvest. The knowledge gained from this study provides a better understanding of climate variability and shelterwood harvesting influences on ecosystem respiration and can be useful for forest managers focused on carbon sequestration and forest conservation. / Dissertation / Doctor of Science (PhD) / Coniferous forest plantations in eastern North America are undergoing silvicultural management to enhance their carbon sequestration capabilities and native-tree species diversity. This study investigated the combined influence of climate oscillations and shelterwood harvesting on soil carbon dynamics of a planted pine forest in southern Ontario, Canada. Between pre- and post-harvesting, soil temperature and soil moisture did not show any significant differences. However, soil CO2 effluxes in post-harvesting years were lower than pre-harvesting years. A Gaussian-Gamma specification model determined that heterotrophic (autotrophic) respiration decreased (increased) post-harvesting and mineral-soil respiration was similar between pre- and post-harvesting. An increase in ecosystem respiration post-harvesting, despite soil CO2 efflux decreasing and being the largest component, was primarily caused by the increase in autotrophic respiration due to enhancement in forest growth. This study improved the understanding of forest carbon dynamics by highlighting the importance of accounting for all components, which may contribute to ecosystem respiration. Results can be useful for forest management practitioners, specifically those focused on carbon sequestration and forest conservation.

Foodborne outbreaks have continued to be associated with produce contamination originating from on-farm sources, such as soil or agricultural water. Additionally, the heterogeneity of the pre-harvest environment complicates the development of universal strategies for managing produce safety risks. Understanding the ecology and epidemiology of foodborne pathogens and fecal indicator bacteria (FIB) by growing regions, sample types, scale of analysis, and detection method is essential for developing targeted mitigation strategies. This dissertation utilized quantitative research methods and statistical modeling to examine the impact of sampling method, spatial, temporal, meteorological, and physicochemical factors on pathogen prevalence and FIB levels. Key findings highlight that the drivers of prevalence differ between pathogens and were influenced by sample type, scale, and region.. The variations in associations emphasize that risk varies by space and time. Therefore, results support regional and scale-dependent food safety standards and guidance documents for controlling hazards to minimize risk. Additionally, the method used for pathogen detection influences prevalence highlighting the need for standard methods since methodological differences confound comparisons across studies. Furthermore, since agricultural water quality is an important food safety priority, this dissertation aimed to determine the efficacy of chemical antimicrobial sanitizers against Salmonella in pre-harvest agricultural water. Results demonstrated that certain sanitizer treatments and conditions can significantly reduce Salmonella populations in preharvest agricultural water sources and thus may serve as a risk reduction option when used correctly. / Doctor of Philosophy / Fresh fruits and vegetables are continually implicated in foodborne outbreaks. Additionally, the source of the pathogen that causes illness in these outbreaks is often due to contact with contaminated soil or water on the farm. Since the environment is extremely diverse, the risk of foodborne pathogens is not uniform across a farm and between farms. Therefore, the development of a one-size-fits-all plan to reduce the risk of foodborne pathogens from contaminating produce on a farm is difficult. Understanding the incidence and distribution of foodborne pathogens and fecal bacteria and how these microorganisms interact with the environment is important to develop strategies to manage risk. Additionally, understanding how the prevalence of bacteria varies by state, medium (water vs soil), and farm is needed to develop targeted mitigation plans. This dissertation utilized laboratory and field-based experiments to understand how space, time, weather, and physical properties impact the occurrence of foodborne pathogens and fecal bacteria. The primary results show factors that impact prevalence are different between pathogens (Salmonella vs Listeria vs E. coli). Furthermore, the occurrence differed by sampling method (molecular vs culture), sample type (water vs soil), scale (within a farm vs between multiple farms), and region emphasizing that the risk from foodborne pathogens varies over space and time. Overall, this dissertation's results suggest that both regional and scale-specific guidelines are needed to reduce foodborne pathogen risks in the farm environment. Lastly, since the quality of the water used in growing fresh produce is an important food safety priority, the effectiveness of chemical antimicrobial sanitizers against Salmonella in agricultural water was evaluated. Results demonstrated that certain sanitizer treatments and conditions (sanitizer concentrations, water temperatures) can significantly reduce Salmonella populations in pre-harvest water sources and may serve as a risk reduction option when sanitizers are used correctly.

The overall goal of this project was to provide baseline data on amphibian species richness, relative abundance, and habitat use for a long-term landscape ecology study on MeadWestvaco industrial forest in the Allegheny Highlands of West Virginia. From results of area-constrained daytime searches (10 m x 10 m plots) across the landscape, I developed 9 regression models to predict amphibian relative abundance. I constructed models for each year for all plots on all habitat types, plots that were in a Stream Management Zone (SMZ), and plots that were in upland, or non-SMZ, habitat. Distance to perennial or ephemeral streams or perennial ponds (SMZ classification), the amount of available rocks along transects, and site index were the 3 most important habitat variables in models for all plots combined and were responsible for 24-32% of the inherent variation in population relative abundance. Other habitat variables that were significant in models were year, % canopy cover, the amount of available woody debris of decomposition classes 3-5 along transects, % woody stems (<7.5 cm DBH), soil pH, and % herbaceous vegetation. R2PRESS values for all 9 models ranged from 0.08 to 0.35. Amphibian relative abundance showed positive relationships with all significant habitat variables with the exception of year and % woody stems. In natural cover object use/availability analyses, I discovered salamanders preferred rocks over woody debris, relative to the amount available of each. Salamanders preferred flat rocks to any other shape, flagstones to any other type of rock, and rock lengths in the 31-40 cm class. Preferred wood widths were in class 5-10 cm, while preferred wood lengths were in class <50 cm; salamanders exhibited strong preferences for wood in higher states of decomposition (class 3-5). I provided baseline, preharvest data for 28-acre reference areas on 9 forest compartments scheduled for clearcuts. I sampled all 9 reference areas preharvest and sampled 3 during year 1 postharvest using coverboard and night plot surveys. On these 3 areas, species richness declined from preharvest to postharvest, but species diversity showed little change. Overall relative abundance declined significantly preharvest to postharvest with coverboard sampling (p=0.0172) and night plot sampling (p=0.0113). At coverboard stations, relative abundance declined significantly from preharvest to postharvest at a distance of 5-10 m (p=0.0163) and 40-50 m (p=0.0193) away from adjacent mature forest. Finally, using Pianka's index, I compared the night plot and coverboard sampling techniques in terms of proportions of the 4 most common species captured. These sampling techniques on average were >80% similar for all reference areas. / Master of Science

Harvest weed seed controls (HWSC) destroy weed seeds that are retained by the plant at crop harvest, which would typically be spread by the harvester along with other field residues. HWSC exploits coincidental maturity between crops and weeds, so an experiment was designed to collect weed seeds as they shatter throughout the growing season and through a simulated harvest delay. This experiment monitored four economically important broadleaf species and two grass species in a soybean (Glycine max (L.) Merr.) field. Results indicated that broadleaf species shattered seed at rates accelerating through the growing season, while grass species shattered more seed early in the growing season. Field experiments in organic and conventional winter wheat (Triticum aestivum L.) fields infested with Italian ryegrass (Lolium perenne L. ssp. multiflorum (Lam.) Husnot) compared two HWSC techniques to grower-standard weed management programs in each system, including both no-till and full-till standard treatments in the conventional system. Italian ryegrass populations were monitored, and wheat yield was measured both before and after HWSC application. In both organic and conventional cropping systems, HWSC treatments did not provide better Italian ryegrass control than the grower-standard treatments. The conventional program including tillage boosted Italian ryegrass populations. These results suggest that HWSC treatments did not enhance Italian ryegrass control compared to grower-standard practices in either the organic or conventional systems. Additionally, broadleaf weeds may retain enough seeds to be viable targets for HWSC. Incorporating best practices, such as a timely crop harvest, is key for understanding and optimizing HWSC. / Master of Science

Two lipid-based edible coatings, Apex B (AC Humko, Memphis, TN) and Durafresh (Pacrite, Ecoscience Produce Systems, Orlando, Fl) were evaluated for their ability to preserve post harvest quality changes in green bell peppers (Capsicum annum L. cv. King Arthur). Post harvest storage quality conditions tested included respiration rates, weight, color and texture changes, and stability of ascorbic acid (AA) and dehydroascorbic acid (DHA) content. Results indicated that no appreciable changes between days or treatment groups occurred in three of the parameters tested: weight, texture, and hue angle (p>0.05 for all parameters). Significant weekly changes were seen in respiration rates, dehydroascorbic acid content, and chromaticity values. Respiration rates and DHA were significantly higher during the last two weeks of the study (p=0.0001, p=0.0001 respectively). Chromaticity values were significantly lower, indicating a more faded color on the 14th day of the study alone (p=0.0097). Initial AA levels were much lower than expected (average initial content=78.72mg/100g). Significant differences between coated and uncoated pepper groups were seen in AA and DHA levels. AA content was found to be significantly lower in coated peppers (p=0.0279), while DHA levels were significantly higher in coated groups (p=0.0126). Overall, coated groups differed little from uncoated counterparts, except in the area of vitamin content in which the coated peppers showed an increase in vitamin breakdown. Despite the results, modifications of lipid coatings are needed, such as creating bilayer and composite coatings that contain either polysaccharide or protein constituents to enhance coating effectiveness. / Master of Science

A floating treatment wetland (FTW) is an ecological approach which seeks to reduce point and nonpoint source pollution by installing substrate rooted plants grown on floating mats in open waters. While relatively novel, FTW use is increasing. A review of literature identified several research gaps, including: (1) assessments of the treatment performance of FTWs; (2) evaluations of FTWs in the U.S., particularly within wet ponds that receive urban runoff; and (3) plant temporal nutrient distribution, plant growth rate, and the long-term persistence of the FTWs in temperate regions with periodic ice encasement. An assessment model, i-FTW model, was developed, and its parameter s fitted based on data from 14 published FTW studies in the first research topic. The estimated median FTW apparent uptake velocity with 95% confidence interval were 0.048 (0.018 - 0.059) and 0.027 (0.016 - 0.040) m/day for total phosphorus (TP) and total nitrogen (TN), respectively. The i-FTW model provided a more accurate prediction in nutrient removal than two common performance metrics: removal rate (mg/m2/day) and removal efficiency (%). In the second research topic, the results of a mesocosm experiment indicated that FTWs with 61% coverage, planted with pickerelweed (Pontederia cordata L.) or softstem bulrush (Schoenoplectus tabernaemontani), significantly improved TP and TN removal efficiency of the control treatment by 8.2% and 18.2%, respectively. The pickerelweed exhibited significantly higher phosphorus and nitrogen removal than the softstem bulrush when water temperatures were greater than 25 deg C. Field observations in the third research topic found that pickerelweed demonstrated higher phosphorus removal performance (7.58 mg/plant) than softstem bulrush (1.62 mg/plant). Based on the observed seasonal changes in phosphorus distribution, harvest of above-ground vegetation is recommended to be conducted twice a year in June and September. Planted perennial macrophytes successfully adapted to stresses of the low dissolved oxygen (DO) concentrations (minimum: 1.2 mg/L), ice encasement, and relatively low nutrient concentrations in the water (median: 0.15 mg/L TP and 1.15 mg/L TN). Systematic observation of wildlife activities indicated eight classes of organisms inhabiting, foraging, breeding, nursing, or resting in the FTWs. Recommendations for FTW design and suggestions for further research are made based upon these findings. / Ph. D.