Spelling suggestions: "subject:"heatshock"" "subject:"plateshock""
421 |
Identifying a role for heat shock proteins in Schistosoma mansoniIshida, Kenji 06 September 2017 (has links)
No description available.
|
422 |
Determining the Location of Heat Shock Protein 70 in Herpes Simplex Virus Type-1 Infected HeLa CellsBagheri, Jordan Pari January 2018 (has links)
No description available.
|
423 |
Identification of Heat Shock Protein 60 as the Ligand on <i>Histoplasma Capsulatum</i>Long, Kristin Helene 21 May 2002 (has links)
No description available.
|
424 |
The Role of Inhibitor-1 and Heat Shock Protein 20 in Cardiac PathophysiologyNicolaou, Persoulla January 2008 (has links)
No description available.
|
425 |
The Role of Grp170 in SP-C<sup>Δexon4</sup> ERADJameel, Amer 05 August 2010 (has links)
No description available.
|
426 |
Molecular Responses to Environmental Stress in Temperate and Polar FliesLopez-Martinez, Giancarlo 24 June 2008 (has links)
No description available.
|
427 |
ADENOSINE RECEPTOR MEDIATED PROTEIN KINASE C ACTIVATION IN THE HEARTYang, Zhaogang 25 June 2012 (has links)
No description available.
|
428 |
The combined effects of thermal and radiological stress on the embryonic development of lake whitefish (Coregonus clupeaformis)Kulesza, Adomas January 2017 (has links)
Lake whitefish (Coregonus clupeaformis; LWF) are a cold-adapted freshwater species that are of both economic and cultural value. These fish spawn in lake areas where their embryos are exposed to thermal power plant effluents that may contain low levels of thermal, radiological and chemical stressors. Many studies on LWF embryonic development have looked at the individual effects of these stressors, but few have looked at the potential for combined effects. The combined effects of thermal and radiological stress were of interest due to growing evidence that mild thermal stress can produce an adaptive response, through the induction of the heat shock response (HSR), when followed with subsequent ionizing radiation stress. This thesis examined the combined impacts of thermal and radiological stress during LWF embryogenesis. LWF embryos were exposed to mild heat shocks (HS; Δ3 or 9°C) prior to a high dose of acute 137Cs gamma rays at 2, 6 and 24 hours post heat shock during the gastrulation or eyed stage. Heat shocked embryos were collected at each developmental stage and assessed for induction of heat shock protein (Hsp) genes. Following exposure, embryos were raised until hatch where mortality, morphometry, and embryo weight were measured. Mild HS induced Hsp70 mRNA expression at gastrulation, but not at the eyed stage. Embryos at hatch were not impacted by thermal or radiological exposure at the gastrulation stage. During the eyed stage, acute radiation treatment increased mortality and decreased body size at hatch. Mild HS prior to radiation did not provide protective effects and no adaptive response was observed. This thesis better defines the combined effects of thermal and radiological stress on the embryonic development of LWF. It also suggests that the ontogeny aspects of heat shock responses and radiosensitivity are important to consider for future adaptive response studies. / Thesis / Master of Science (MSc)
|
429 |
Studies on Genomic Sequences For the Heat Shock Proteins hsp60 and hsp10 From Chinese Hamster Ovary CellsZurawinski, Joni 12 1900 (has links)
Although the eDNA sequences for the 10 k:Da (hsp 10, hsp 1 0) and the 60 k:Da (hsp60,
cpn60) heat shock proteins have been obtained for a number of mammalian species, until very
recently information was not available on the functional genes encoding these proteins. The
primary objective of this work was to clone and sequence the functional genes for these
proteins from CHO, Chinese hamster ovary cells. Screening of a lambda EMBL3 CHO
genomic library with the CHO hsp 10 eDNA identified a clone containing the putative hsp 10
functional gene. A -5.5 kb fragment was isolated from one of these clones by enzymatic
digestion and -3.3 kb was sequenced. The clone was found to contain consensus regulatory
sequences upstream of the putative transcription initiation site, + 1, including two Sp 1 binding
sites, a CAAT box, and a single heat shock element, HSE, but lacked a TATA box. The
coding region consists of four exons, identical to the hsp10 CHO eDNA sequence, separated
by three introns, of 200 bp, 600 bp and 1600 bp in size, containing conserved splice sites.
Screening of the same EMBL3 CHO genomic library with the CHO hsp 10 eDNA also
resulted in isolation of a full length processed pseudogene with -90 % identity to the eDNA.
This pseudogene lacked introns, contained a poly(A) tract, as well as various single bp
changes, additions and deletions. The upstream region of this pseudo gene was found to
contain similarity to the human LINE sequence, a DNA repetitive element. PCR amplification
ofCHO-WT genomic DNA resulted in isolation offive additional processed pseudogenes,
corresponding to the central -270 bp of the CHO hsplO eDNA. All the pseudogenes
displayed a high degree of similarity to the CHO hsp 10 eDNA sequence despite the presence
of numerous mutations. Prior to this report, pseudogenes had not been found associated with
hsp 10. The identification of these pseudogenes suggests the presence of a multi gene family
for this heat shock protein in the CHO genome.
Previously, a semi-processed pseudogene, Gel, was identified for hsp60 from CHO
cells which contained a single -87 bp intron near its 3' end (Venner eta/., 1990). From this
pseudo gene, a fragment containing the -87 bp intron was isolated for use as a probe to screen
a lambda EMBL3 CHO genomic library. This resulted in isolation of several positive clones,
two of which were purified, a -1.0 kb fragment amplified by PCR and then sequenced
revealing two additional semi-processed pseudogenes, designated .A4 and .AS. These
pseudo genes were found to be homologous to the GC 1 clone, containing many similar
mutations as well as the -87 bp intron. Utilizing CHO-WT genomic DNA, a separate PCR
amplification resulted in isolation of a -2.5 kb fragment which was partially sequenced and
found to correspond to the putative hsp60 functional gene. The fragment contained one
exon, which was identical to the CHO hsp60 eDNA in the region sequenced, and two introns
of800 bp and 1500 bp. This fragment can now provide an ideal probe for isolation ofthe
CHO hsp60 functional gene. / Thesis / Master of Science (MSc)
|
430 |
Minimum Ultraviolet Light Dose Determination and Characterization of Stress Responses that Affect Dose for Listeria monocytogenes Suspended in Distilled Water, Fresh Brine, and Spent BrineMcKinney, Julie 29 April 2008 (has links)
Foodborne illnesses caused by Listeria monocytogenes have long been associated with ready-to-eat (RTE) meats contaminated after the primary thermal process has been applied. It is believed that brine solutions used to chill cooked RTE products may serve as a reservoir for L. monocytogenes becoming a potential point of post-processing contamination for RTE meats. Re-circulating ultraviolet light (UV) systems are being used to inactivate L. monocytogenes in chill brines; however very little has been reported on the dose response of healthy and stressed L. monocytogenes to UV in brine solutions. The objectives of this research were to determine 1) minimum dose of UV required to inactivate L. monocytogenes in distilled water, fresh brine, undiluted spent brine, and diluted spent brine, 2) if adaptation to food processing stresses affects the dose response, and 3) if the acquisition of antibiotic resistance mechanisms provides resistance to ultraviolet light 4) effect of stress adaptation on survival in brine solutions. After UV exposure, populations were reduced as follows from greatest to least: water > fresh brine > 5% spent brine > 35% spent brine > 55% spent brine > 100% spent brine (P ≤ 0.05). There were no population differences between acid stressed and antibiotic resistant or healthy and heat shocked (P > 0.05). However, acid-stressed and sulfanilamide-resistant were more resistant to UV light than healthy and heat shocked L. monocytogenes (P ≤ 0.05). Survival in brine solutions (no UV) followed the trend, from greatest to least (P ≤ 0.05): sulfanilamide-resistant > acid-stressed > healthy > heat-shocked. Population estimates decreased from initial inoculation to final sampling for each cell type suspended in spent brine (P ≤ 0.05), but only healthy and heat- shocked cells suspended in fresh brine were significantly reduced (P ≤ 0.05). Knowledge of UV dosing required to control L. monocytogenes in brines used during RTE meat processing, and a greater understanding of the interactions that may influence dose will aid manufacturers in establishing appropriate food safety interventions for these products. / Ph. D.
|
Page generated in 0.0213 seconds