Resumo:Neste estudo foram comparados os valores do hemograma completo realizado em 30 muares com idade entre 4 e 8 anos, obtidos pelo uso de um analisador hematológico automático e pela contagem manual de células sanguíneas. A análise dos resultados revelou que a concordância entre os dois métodos foram considerados bons uma vez que o viés (média das diferenças) foi pequeno, sendo que os intervalos de confiança de 95% para o viés apresentaram-se estreitos e apenas um "outlier" foi observado, para eritrócitos, monócitos e granulócitos (nenhum valor caiu fora dos limites de concordância [viés ± 2 desvios padrão]). Não houve diferença significativa para os valores de eritrócitos, hemoglobina, leucócitos e granulócitos. Os valores médios de monócitos obtidos pelo contador automático de células foram significativamente maiores do que aqueles obtidos pelo método manual. A análise dos resultados revelou ainda um viés positivo para hematócrito e nenhum viés foi detectado para eritrócitos, hemoglobina, leucócitos, granulócitos e linfócitos. Foi observada boa concordância entre os resultados obtidos pelos dois métodos para eritrócitos, hemoglobina, leucócitos, granulócitos, linfócitos e monócitos nessa faixa etária. Podendo o Analisador hematológico automático BC- 2800Vet®, ser utilizado para realização de hemogramas em muares / Abstract:In this study the values of complete blood counts performed in 30 mules aged 4 and 8, obtained by using an automated hematology analyzer and the manual blood counts were compared. The results showed that the correlation between the two methods were considered good since the bias (mean difference) was small, and the confidence intervals of 95 % for the bias showed up narrow and only one "outlier" was observed for erythrocytes, monocytes and granulocytes (no value fell outside the limits of agreement [bias ± 2 standard deviations]). There was no significant difference in the values of erythrocytes, hemoglobin, leukocytes and granulocytes. The average values obtained from monocytes by an automatic cell counter were significantly higher than those obtained by the manual method. The results also showed a positive bias for hematocrit and no bias was detected in erythrocytes, hemoglobin, leukocytes, granulocytes and lymphocytes. Good agreement between the results obtained by the two methods for erythrocytes, hemoglobin, leukocytes, granulocytes, lymphocytes and monocytes was observed in this age group. Can the automated hematology analyzer BC-2800Vet® be used to perform complete blood counts in mules / Orientador:Luiz Cláudio Nogueira Mendes / Banca:Lina Maria Wehrle Gomide / Banca:Raimundo Souza Lopes / Mestre

Made available in DSpace on 2015-10-06T13:03:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-02-28. Added 1 bitstream(s) on 2015-10-06T13:18:46Z : No. of bitstreams: 1 000849287.pdf: 230262 bytes, checksum: 43cb89c8000a95aa870bb4e1a18fdf53 (MD5) / In this study the values of complete blood counts performed in 30 mules aged 4 and 8, obtained by using an automated hematology analyzer and the manual blood counts were compared. The results showed that the correlation between the two methods were considered good since the bias (mean difference) was small, and the confidence intervals of 95 % for the bias showed up narrow and only one outlier was observed for erythrocytes, monocytes and granulocytes (no value fell outside the limits of agreement [bias ± 2 standard deviations]). There was no significant difference in the values of erythrocytes, hemoglobin, leukocytes and granulocytes. The average values obtained from monocytes by an automatic cell counter were significantly higher than those obtained by the manual method. The results also showed a positive bias for hematocrit and no bias was detected in erythrocytes, hemoglobin, leukocytes, granulocytes and lymphocytes. Good agreement between the results obtained by the two methods for erythrocytes, hemoglobin, leukocytes, granulocytes, lymphocytes and monocytes was observed in this age group. Can the automated hematology analyzer BC-2800Vet® be used to perform complete blood counts in mules

<p>Platelets are the smallest cells in the blood. They are formed in the bone-marrow and are important for the blood coagulation. Platelet tranfusions are given to patients propyhlactically before an operation but also in therapeutical purpose in connection with bleeding. It’s importent that the quality controls of the platelet concentrates are reliable.</p><p>ADVIA®60 (Bayer HealthCare) is a fully automated cell counter which uses impedance principle to count platelets in blood samples. The purpose of the study was to evaluate this new instrument for use in the blood bank of Akademiska Sjukhuset in Uppsala. The instrument was bought to be used for quality control of platelet concentrates. 30 samples from platelet concentrates, from both apheresis and from buffy coats, were analyzed 10 times each on ADVIA®60 and the coefficient of variation (CV) was calculated for each sample. CV variated from 0,8 % to 2,9 % which is good considering that according to Bayer HealthCare the CV should be < 5 % for thrombocytes on ADVIA®60. The instrument was newly calibrated when the study was performed. Platelet count can also be performed by immunological or optical principles.</p>

Platelets are the smallest cells in the blood. They are formed in the bone-marrow and are important for the blood coagulation. Platelet tranfusions are given to patients propyhlactically before an operation but also in therapeutical purpose in connection with bleeding. It’s importent that the quality controls of the platelet concentrates are reliable. ADVIA®60 (Bayer HealthCare) is a fully automated cell counter which uses impedance principle to count platelets in blood samples. The purpose of the study was to evaluate this new instrument for use in the blood bank of Akademiska Sjukhuset in Uppsala. The instrument was bought to be used for quality control of platelet concentrates. 30 samples from platelet concentrates, from both apheresis and from buffy coats, were analyzed 10 times each on ADVIA®60 and the coefficient of variation (CV) was calculated for each sample. CV variated from 0,8 % to 2,9 % which is good considering that according to Bayer HealthCare the CV should be &lt; 5 % for thrombocytes on ADVIA®60. The instrument was newly calibrated when the study was performed. Platelet count can also be performed by immunological or optical principles.

Hematology analyzers can be used for screening patients for blood abnor-malities. The techniques used in a hematology analyzer include impedanceanalysis, flow cytometry and spectroscopy, which allow for measuring of forexample absolute count, sizes and concentration of different cells in a patient’sblood sample. Hyperlipidemia, which refers to elevated blood lipid levels, isthe primary cause of heart-related illness and fatalities in today’s developedor developing countries. Currently, blood lipid levels are not measured as aparameter with hematology analyzers. Since hematology analyzers allow for arapid general screening of blood parameters, an area of interest is therefore tobe able to measure blood lipids with a hematology analyzer. Thus, this studyaims to investigate the possibility of detecting and measuring blood lipids witha hematology analyzer, using flow cytometry and/or spectrophotometry. In order to investigate this possibility, two simulating methods were conductedwhere in the first method Intralipid 20% was mixed with saline into sampleswith different lipid concentrations. In the second method, diluent wasused instead of saline. Lastly a Correlation study was performed whereIntralipid 20% was mixed with donor blood to prepare samples with differentlipid concentrations. All samples were then analyzed in a hematologyanalyser and scatter plots from flow cytometry and light absorption datafrom spectrophotometry measurements were obtained. The methods showedthat there is a strong correlation between number of detected pulse countsfrom the scatter plots and lipid concentration. Same applies to lightabsorption compared to the lipid concentration of the samples, measured withspectrophotometry. The results from this study show that it is in fact possible to detect andmeasure blood lipid levels with a hematology analyser using flow cytometryand spectrophotometry. Further development within this area could thereforeenable simple screening of this additional parameter and early detection ofindications of hyperlipidemia. / Hematologianalysatorer möjliggör screening av eventuella avvikelser ipatienters blood. De tekniker som används i en hematologianalysatorinkluderar impedansanalys, flödescytometri och spektroskopi, vilka möj-liggör mätning av till exempel absolutantal, storlekar och koncentrationav olika celler i ett patientblodprov. Hyperlipidemi, vilket hänvisar tillförhöjda blodlipidnivåer, är den främsta orsaken till hjärtrelaterade sjukdomaroch dödsfall i dagens utvecklade eller utvecklingsländer. För närvarandemäts inte blodlipidnivåer som en parameter med hematologianalysatorer.Eftersom hematologianalysatorer möjliggör en snabb allmän screening avblodparametrar, är ett intresseområde därför att kunna mäta blodlipider meden hematologianalysator. Syftet med denna studie är därför att undersökamöjligheten att detektera och mäta blodlipider med en hematologianalysator,med hjälp av flödescytometri och/eller spektrofotometri. Två simuleringsmetoder genomfördes, där den första metoden innefattadeblandning av Intralipid 20% med saltlösning till prover med varierandelipidkoncentration. I den andra metoden användes spädningsvätska iställetför saltlösning. Slutligen genomfördes en korrelationsstudie där Intralipid20% blandades med donatorblod och prover med olika lipidkoncentrationerförbereddes. Alla prover analyserades sedan i en hematologianalysator ochspridningsdiagram och ljusabsorptionsdata från spektrofotometrimätningarerhölls. Resultaten visade att det finns en stark korrelation mellan antalet de-tekterade pulsräkningar från spridningsdiagrammen och lipidkoncentrationen.Samma gäller för ljusabsorption jämfört med lipidkoncentrationen i proverna,mätt med spektrofotometri. Resultaten från denna studie visar att det faktiskt är möjligt att detektera ochmäta blodlipidnivåer med en hematologianalysator med hjälp av flödescyto-metri och spektrofotometri. Vidare utveckling inom detta område skulle därförkunna möjliggöra enkel screening av patienters blod lipidkoncentration samtunderlätta en tidig upptäckt av indikationer på hyperlipidemi.

[pt] Os analisadores hematológicos são sistemas de medição que permitem identificar elementos sanguíneos, incluindo a contagem diferencial dos cinco tipos de leucócitos encontrados no sangue periférico: neutrófilos, linfócitos, monócitos, eosinófilos e basófilos. Devido à inexistência de materiais de referência certificados ou métodos de referência para a análise diferencial de leucócitos, tem sido habitual avaliar o desempenho de novos analisadores comparando-os com sistemas tradicionalmente disponíveis e bem consolidados. Em 2015, foi lançada uma nova tecnologia portátil e de baixo custo, o modelo DxH500 (Beckman Coulter), baseada na perda axial de luz usando LED azul como emissor, combinada à medição de impedância. O presente trabalho compara o desempenho do DxH500 com um modelo bastante utilizado desde seu lançamento em 2001 para exames laboratoriais em larga escala, o modelo LH750 (Beckman Coulter), baseado na combinação dos princípios de impedância, condutividade e dispersão de luz (VCS). No estudo foram examinadas 310 amostras pareadas. A análise comparativa entre os resultados fornecidos por cada dispositivo indicou uma boa correlação para a caracterização de neutrófilos, linfócitos, monócitos e eosinófilos. Apesar da reduzida correlação observada para a contagem de basófilos, esse resultado não apresenta relevância clínica já que os valores obtidos para as amostras avaliadas foram muito reduzidos, inferiores aos limites de referência. Embora a análise comparativa realizada tenha apontado para um desempenho equivalente utilizando-se tecnologias com princípios diversos, a adequada avaliação dos dispositivos de análise hematológica requer o desenvolvimento de materiais de referência certificados, uma demanda fundamental para a garantia da confiabilidade na quantificação diferencial de leucócitos. / [en] Hematology analyzers are measurement systems that allow the identification of blood elements, including differential counting of the five types of leukocytes found in peripheral blood: neutrophils, lymphocytes, monocytes, eosinophils, and basophils. Because of the lack of certified reference materials or reference methods for differential leukocyte analysis, it has been customary to evaluate the performance of new analyzers by comparing them with traditionally available and well-established systems. In 2015, a new low-cost, portable technology was introduced, the Beckman Coulter DxH500 model, based on the axial loss of light using blue LED as a combined transmitter for impedance measurement. The present work compares the performance of the DxH500 with a widely used model since its launch in 2001 for large-scale laboratory tests, the Beckman Coulter model LH750, based on the combination of impedance, conductivity and scatter (VCS). In the study, we examined 310 paired samples. The comparative analysis between differential leukocyte evaluation results provided by each device indicated a good correlation for the characterization of neutrophils, lymphocytes, monocytes, and eosinophils. Despite the low correlation observed for basophil counts, this result is not clinically relevant since the values obtained for the evaluated samples were very low, lower than the reference limits. Although the comparative analysis carried out pointed to an equivalent performance using technologies with different principles, the adequate evaluation of hematological analysis devices requires the development of certified reference materials, fundamental demand for the reliability of the differential quantification of leukocytes.