In order to investigate the effects of C-terminus modification of Dragon Grouper Nervous Necrosis Virus capsid protein on the virus particle formation, we used E. coli expression system to express DGNNV capsid protain with different truncations at C-teminus fused with six or three histidines (His-Tag). These poly-His tagged clones, including ¡µC334-C6H, ¡µC335-C6H, ¡µC336-C6H, ¡µC337-C6H, C3H and C6H (His6 tagged at the C-teminus of wild-type capsid protein)¡Awere expressed and VLPs formation ability were examined. Wild-type and N-terminal recombination (N6H, His6 tagged at the N-teminus of wild-type capsid protein) were also used for comparison. These His-tagged VLPs can be further purified by Ni-NTA agarose, and their thermal stability of mutant VLPs were analyzed by Circular Dichroism. The Western blotting and ELISA assay were utilized to analyzed N-teminus or C-terminus was located at the surface of virus icosahedron. Once the four amino acids at the C-terminus of capsid protein were truncated (¡µC334-C6H), the mutated cpasid protein cannot assemble into VLPs. The same phenomenon was also observed in C6H. The related productions of wild-type, ¡µC335-C6H, ¡µC336-C6H, ¡µC337-C6H, C3H VLPs were about 100%, 56%, 116%, 141%, and 193%, respectively. Using Circular Dichroism to observe the thermal stability of mutant VLPs, the results revealed that the Tm of mutant VLPs were about 3oC lower than wild-type VLPs (61oC). The results of Western blotting and ELISA assay suggest that the C-termius of DGNNV capisid protein was exposed to the surface of virus structure.

Microstrip patch antennas are attractive for numerous military and commercial applications due to their advantages in terms of low-profile, broadside radiation, low-cost, low-weight and conformability. However, the inherent narrowband performance of patch antennas prohibits their use in systems that demand wideband radiation. To alleviate the issue, an existing approach is to combine multiple patch antennas within a log-periodic array configuration. These log-periodic patch antennas (LPMAs) are capable of providing large bandwidths (>50%) with stable broadside radiation patterns. However, they suffer from electrically large sizes. Therefore, their miniaturization without degrading the bandwidth performance holds promise for extending their use in applications that demand conformal and wideband installations. In recent years, electromagnetic band gap structures have been proposed to enhance the radiation performances of printed antennas. These engineered surfaces consist of a periodic arrangement of unit cells having specific metallization patterns. At particular frequencies, they provide a zero-degree phase shift for reflected plane waves and effectively act as high impedance surfaces. Since, their band-limited electromagnetic field behavior is quite similar to a hypothetical magnetic conductor; they are also referred to as artificial magnetic conductors (AMCs). AMC structures were shown to allow lower antenna profile, larger bandwidth, higher gain, and good unidirectional radiation by alleviating the field cancellation effects observed in ground plane backed antenna configurations. Previous research studies have already demonstrated that microstrip patch antennas can enjoy significant size reductions when placed above the AMC surfaces. This project, for the first time, investigates the application of AMCs to LPMA configurations. Specifically, the goal is to reduce the LPMA size while retaining its highly desired large bandwidth performance. To accomplish this, we employ various AMC surface configurations (e.g. uniform, log-periodic) under traditional LPMAs and investigate their performance in terms of miniaturization, bandwidth, gain, and radiation patterns.

Professor Pieter Marais, Executive Dean of the Tshwane University Technology’s (TUT) Faculty of Science, received a special award from the Director-General of the Department of Science and Technology, Dr Phil Mjwara, at a breakfast meeting of the South African Council for Professional Natural Scientists (SACNASP) recently

Η είσοδος της πληροφορικής στην ζωή μας ήταν καθοριστική πόσο μάλλον και στον τομέα της Υγείας. Τα σύγχρονα Πληροφοριακά Συστήματα Νοσοκομείου (ΠΣΝ) διαδραματίζουν σημαντικό ρόλο στη βελτίωση των υπηρεσιών υγείας και στην καθημερινή διευκόλυνση τόσο του νοσοκομειακού προσωπικού όσο και των πολιτών. Η χρήση αυτών απλοποιεί τις υφιστάμενες διαδικασίες λειτουργίας των μονάδων υγείας, ελαττώνει τον καθημερινό φόρτο εργασίας, βελτιώνει σημαντικά τις συνθήκες εργασίας του προσωπικού και μειώνει αισθητά το χρόνο αναμονής του ασθενή. Παρόλα αυτά στη χώρα μας η εφαρμογή τους στα δημόσια νοσοκομεία εμφανίστηκε σταδιακά και ακόμα και σήμερα η χρήση τους δεν είναι ευρέως διαδεδομένη. Στην παρούσα διπλωματική εργασία αρχικά περιγράφεται η δομή και τα βασικά χαρακτηριστικά ενός Πληροφοριακού Συστήματος Νοσοκομείου. Στην συνέχεια, εξετάζεται η εφαρμογή και η χρήση ενός συγκεκριμένου Πληροφοριακού Συστήματος ‘ΑΣΚΛΗΠΙΟΣ™ HOSPITAL’ στο Νοσοκομείο του Μεσολογγίου όπου και έχει εγκατασταθεί. Για την εργασία αυτή πραγματοποιήθηκε έρευνα στο Γενικό Νοσοκομείο Μεσολογγίου «ΧΑΤΖΗΚΩΣΤΑ» με σκοπό τη διάγνωση προβλημάτων στην ανάπτυξη και την εφαρμογή του Πληροφοριακού Συστήματος ‘ΑΣΚΛΗΠΙΟΣ™ HOSPITAL’ προκειμένου μελλοντικά να επιλυθούν. / The entry of information into our lives was decisive let alone into the field of health. Current Hospital Information Systems (HIS) are significant and imperative in improving health services and facilitate daily both hospital staff and citizens. Using these simplifies the existing operating procedures of health facilities, reduces the daily workload, significantly improves the working conditions of staff and reduces the waiting time of patient. However, in our country their use in public hospitals has been appeared progressively and even today is not widespread. In this present diploma thesis, it is given a description of the structure and the main characteristics of a Hospital Information System (HIS). Then, is considered the application and the use of a specific Information System 'ASKLIPIOS ™ HOSPITAL' into Hospital of Messolonghi where has been installed. Within elaborating this paper a survey has been conducted in General Hospital of Messolonghi « HATZIKOSTA » with purpose of diagnosing problems in the development and implementation of the Information System 'ASKLIPIOS ™ HOSPITAL' in order future to be resolved.

This thesis project examines Philip Pullman’s controversial trilogy, His Dark Materials (1995-2000), and its relationships in theme and content to John Milton's Paradise Lost (1667). Though Pullman has publicly discussed the influences of Paradise Lost on his trilogy, very little academic work has been published examining the similarities and differences between the two. Specifically, I analyze the paradigms of gender, family, and authority as they are represented by each text. I contrast Pullman's Lyra to Milton's Eve, drawing conclusions about the inherent meanings and differences in the two female protagonists and, consequently, the narrative worlds surrounding them. References cited include works examining His Dark Materials, Paradise Lost, and children's literature.

No description available.

Ribosomal stalk proteins are known to play important role in protein synthesis. The ‘stalk’, an extended structure on the large subunit of the ribosome is composed mainly of two to three dimers of L12 and one L10 protein, which forms the base of the stalk. In E. coli, four copies of L12 molecules exist as dimer of dimers forming the pentameric L8 complex together with L10. This thesis is a collection of four interlinked studies on the structure, function and application of the ribosomal stalk protein L12. In the first study, we have mapped the interaction sites of the four major translation GTPase factors (IF2, EF-Tu, EF-G & RF3) on L12 molecule using heteronuclear NMR spectroscopy. Surprisingly, all these factors produced an overlapping interaction map spanning two α-helices on the C terminal domain of L12, thereby suggesting a general nature of the interaction between L12 and the GTPase factors. L12 is known to stimulate GTPase activity of the elongation factors EF-Tu and EF-G. Here, we have clarified the role of L12 in IF2 mediated initiation of protein synthesis. Our data suggest that rapid subunit association requires a specific interaction between the L12 protein on the 50S and IF2·GTP on the 30S preinitiation complex. We have also shown that L12 is not a GAP for IF2 and GTP hydrolysis triggers IF2 release from the 70S initiation complex. The next question we have addressed is why multiple copies of L12 dimer are needed on the ribosome. For this purpose, we created a pure E. coli strain JE105, where the terminal part of rplJ gene coding for the binding site of one L12 dimer on protein L10 was deleted in the chromosomal locus. Using ribosomes with single L12 dimer we have observed that the rate of the initiation and elongation involving IF2 and EF-G gets most compromised, which in turn decreases the growth rate of the bacteria.  This study also indicates that L12 can interact with different GTPase factors in a specialized manner. Lastly, we have developed an application making advantage of the multiple L12 dimers on the ribosome. By inserting a (His)6-tag at the C-terminus of the L12 protein we have created a novel E. coli strain (JE28), where all ribosomes are tetra-(His)6-tagged. Further, we have developed a single step method for purification of the active (His)6-tagged ribosomes from JE28.

Die Behandlung von Patienten mit Tumoren im Kopf-Hals-Bereich gestaltet sich als komplexer und herausfordernder Prozess sowohl für den Patienten als auch für die behandelnden Ärzte und Chirurgen. Zur Gewährleistung der bestmöglichen individuellen Therapie werden vor Beginn der Behandlung zahlreiche diagnostische Verfahren durchgeführt. Hierzu zählen unter anderem medizinische bildgebende Verfahren wie z.B. Computertomographie (CT) oder Magnetresonanztomographie (MRT) sowie die Entnahme von tumorverdächtigem Gewebe während einer Panendoskopie zur exakten Bestimmung der Tumorart (Histologie, Grading, TNM-Klassifikation nach UICC, genaue Lokalisation des Primärtumors, der lokoregionären Metastasen und ggf. Fernmetastasen). Die gewonnenen Informationen bilden anschließend die Grundlage für die Entscheidung über die durchzuführende Therapie und stehen in unterschiedlichen klinischen Informationssystemen sowie auf Papierakten zur Verfügung. Leider werden die Daten im klinischen Alltag häufig nur unstrukturiert und schwer auffindbar präsentiert, da die führenden Informationssysteme nur unzureichend in den klinischen Arbeitsprozess integriert und untereinander schlecht vernetzt sind. Die präzise und erschöpfende Darstellung der jeweiligen individuellen Situation und die darauf aufbauende Therapieentscheidung sind aber entscheidend für die Prognose des Patienten, da der erste, gut geplante \"Schuss\" entscheidend für den weiteren Verlauf ist und nicht mehr korrigiert werden kann. In dieser Arbeit werden neue Konzepte zur Verbesserung des Informationsmanagements im Bereich der Kopf-Hals-Tumorbehandlung entwickelt, als prototypische Software implementiert und im klinischen Alltag in verschiedenen Studien wissenschaftlich evaluiert. Die Erlangung eines tiefgreifenden Verständnisses über die klinischen Abläufe sowie über beteiligte Informationssysteme und Datenflüsse stellte den ersten Teil der Arbeit dar. Aufbauend auf den Erkenntnissen wurde ein klinisches Informationssystem oncoflow entwickelt. Oncoflow importiert vollautomatisch relevante Patientendaten von verschiedenen klinischen Informationssystemen, restrukturiert die Daten und unterstützt Ärzte und Chirurgen im gesamten Therapieprozess. Das System wurde anschließend in unterschiedlichen Studien evaluiert und der klinische Nutzen in Bezug auf effizientere Arbeitsabläufe und eine verbesserte Informationsqualität gezeigt. Im folgenden Teil der Arbeit wurden Machine Learning Methoden genutzt um von Daten in der elektronischen Patientenakte auf den aktuellen Prozessschritt im Therapieprozess zu schließen. Der letzte Teil der Arbeit zeigt Möglichkeiten zur Erweiterung des Systems zur Nutzung in weiteren klinischen Fachdisziplinen auf.

For processing the theme of dissertation were prepared two walks and one field trip. 1-he first walk is focused naturally and the second walk is focused geographically. The aim of dissertation is to provide to schoolchildren a contact with a direct surroundings of the school and a place of residence. Chosen activities lead to recognition of \tilevsko and his outskirt.

CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior / FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / O dÃficit habitacional atinge o Brasil desde o final do sÃculo XIX quando se inicia de modo acelerado o processo de crescimento da cidade associado a um vertiginoso aumento demogrÃfico desacompanhado da implantaÃÃo de redes de infraestrutura urbana. Diante deste problema diversas polÃticas pÃblicas vÃm sendo formuladas com o intuito de atender à crescente demanda por moradia e promover o crescimento ordenado de nossas cidades. Todavia a necessidade de abranger um enorme contingente de pessoas faz com que a elaboraÃÃo dos projetos seja pautada na resoluÃÃo quantitativa deste dÃficit mesmo que isso interfira na qualidade desses. Neste contexto esta pesquisa tem como objetivo analisar os projetos arquitetÃnicos destinados à habitaÃÃo de interesse social (HIS) na capital cearense produzidos pela Prefeitura do MunicÃpio por meio da FundaÃÃo de Desenvolvimento Habitacional de Fortaleza (HABITAFOR) no perÃodo de 2003 ano de criaÃÃo do ÃrgÃo citado atà 2012. Esta investigaÃÃo adota como estratÃgia de pesquisa a anÃlise documental realizada em quatro etapas: a coleta de projetos arquitetÃnicos junto a ÃrgÃos competentes bem como a organizaÃÃo do material coletado a apreciaÃÃo dos documentos por meio dos parÃmetros de anÃlise elaborados e logo em seguida, o tratamento desses. A anÃlise demonstrou que os projetos produzidos pela Habitafor em sua maioria, sÃo destinados ao reassentamento de famÃlias residentes em Ãreas de risco. Verificou-se ainda, que as plantas baixas possuem trÃs setores: o social Ãntimo e o de serviÃo caracterÃstica comum na habitaÃÃo brasileira porÃm em nem todos os projetos arquitetÃnicos hà este zoneamento e que o aumento do nÃmero de quartos nÃo implica na extensÃo dos demais ambientes. Os projetos sÃo pouco flexÃveis dado que as plantas propostas pouco favorecem a expansÃo das unidades conforme as necessidades das famÃlias / The housing deficit reaches Brazil since the late nineteenth century when starting an accelerated process of city growth associated with rapid population growth and insufficient implementation of urban infrastructure. Faced with this problem several policies have been formulated in order to meet the growing demand for housing and promote the orderly growth of our cities. However the need to cover a huge number of people makes the preparation of projects is based in the resolution quantitative this deficit even if it interferes with their quality. In this context this research aims to analyze the architectural projects intended for social housing (SH) in capital of Cearà produced by Municipal Prefecture through the Foundation Housing Development in Fortaleza (HABITAFOR) from 2003 the year of creation of the institution cited to 2012. This research adopts as a research strategy the document analysis by performing four steps: collection of architectural projects in the institution responsible and the organization of the material collected appreciation of documents through the analysis parameters developed and soon thereafter treatment of same. The analysis shows that the designs produced by Habitafor mostly are intended to resettle families living in areas at risk. It was found also that the architectural projects have three sectors: social intimate and service a common characteristic in the Brazilian housing but not in all architectural projects have this zoning and that increasing the number of rooms does not imply an extension the other spaces. The projects analyzed are inflexible because the plants analyzed do not favor the expansion of the units according to the needs of families