No description available.

Human milk contains ~ 25 µg/mL of soluble cluster of differentiation 14 (sCD14) protein, a pattern recognition receptor (PRR) that triggers the innate immune system to respond to bacterial lipopolysaccharide (LPS). To date, the role of CD14 in the digestive tract of breast fed infants has not been well characterized and is the subject of this thesis. To investigate the biodistribution of proteins such as CD14 in vivo, a novel method for 14C radiolabeling of proteins to high specific radioactivity was developed using in vacuo methylation. Bovine serum albumin (BSA) and casein were used as test proteins to determine the following: 1) The efficacy of the in vacuo radiolabeling procedure; 2) The extent of incorporation of the 14C-label into the organs of oro-gastric gavaged 10 day old Sprague Dawley rats. [14C]BSA, [14C]casein and [14C]CD14 were prepared with specific radioactivities of 10 400, 10 800 and 163 000 dpm/µg, respectively. After feeding 6.25 µg of 14C-labeled proteins, quantifiable levels of 14C were found in the stomach, jejunum, duodenum, ileum, large intestine, intestinal luminal flushes, blood, liver, spleen and kidneys of rats. The accumulation of radiolabel in the organs of [14C]CD14 fed rats was temporally and spatially distinct from [14C]BSA and [14C]casein. Most notably, the label persisted in the stomach 480 min post-gavage. To design a neonate animal model for biodistribution, the segmental and total gastrointestinal transit times (GItt) were measured in two litters of 10 and 15 day old Sprague Dawley rat pups using barium sulfate. Ten day old rat pups that remained with and without the dam had a total gastrointestinal transit time of 13.8 ± 0.9 hr and 9.3 ± 0.7 hr, respectively. This decrease (p<0.05) in total gastrointestinal transit time in the absence of the dam was age dependent, as it was not observed (p>0.05) in the 15 day old rat pup litter. The immunological impact of an exogenous sCD14 source was examined in human peripheral blood mononuclear cells (PBMC). Pre-treatment of CD14+ monocytes with sCD14 had a protective effect, one of reducing the production of proinflammatory cytokines (TNF-α, IL-6, IL-8, IL-1β) when challenged with LPS. 14C was absorbed by neonate rats upon ingestion of [14C]CD14 and exposure to relatively high concentrations of rCD14 led to a reduction in inflammation. This may be beneficial to initial gut colonization in breast-fed newborns. / Alexander Graham Bell NSERC CGS M scholarship. Japan Society for the Promotion of Sciences, Summer in Japan Fellowship. Funded by the Canadian Institutes of Health Research, Institute of Nutrition Metabolism and Diabetes Grant #82816 “Fate and function of breast milk and recombinant human CD14 at mammary and newborn gastrointestinal mucosal epithelia”.

Substantial evidence supports a role of diet in glucose metabolism, but only a few reports have investigated the impact of diet during pregnancy on risk of gestational diabetes (GDM). Although metabolic hormones have been detected in milk, no studies have investigated the impact of maternal metabolic status assessed during pregnancy on insulin and adiponectin concentrations in human milk. The purpose of this thesis was to investigate the association of maternal macronutrient intakes with metabolic status during pregnancy and its subsequent impact on human milk hormones. Participating women (n=216) underwent a 3-hour oral glucose tolerance test at 30 (95% confidence interval [CI] 25, 33) weeks gestation, recalled their second trimester dietary intake, and donated early (the first week) and mature (3 months postpartum) milk. Higher vegetable and fruit fiber intake was associated with reduced insulin resistance (beta±SE -0.100±0.029, p=0.0008) and increased insulin sensitivity (0.029±0.012, p=0.01) among those with a family history of type 2 diabetes. Lower % carbohydrate and higher % total fat were associated with increased GDM risk (odds ratio 0.60 [95% CI 0.40, 0.90] and 1.61 [1.06, 2.44], respectively). Prenatal metabolic abnormalities including higher pregravid body mass index (beta±SE 0.053±0.014, p=0.0003), in addition to gravid hyperglycemia (0.218±0.087, p=0.01), insulin resistance (0.255±0.047, p<0.0001), lower insulin sensitivity (-0.521±0.108, p<0.0001), and higher serum adiponectin (0.116±0.029, p<0.0001) were associated with higher insulin in mature milk. Obstetrical measures including nulliparity (0.171±0.058, p=0.004), longer duration of gestation (0.546±0.146, p=0.0002), and unscheduled caesarean section (0.387±0.162, p=0.02) were associated with higher adiponectin in early milk. Holder pasteurization, a process recommended by the Human Milk Bank Association of North America before distributing human donor milk, reduced milk adiponectin and insulin concentrations by 32.8% and 46.1%, respectively (both p<0.0001). In conclusion, the distribution of macronutrient intakes during pregnancy was associated with risk for abnormal glucose metabolism later in pregnancy. In addition, maternal prenatal metabolic abnormalities were associated with high insulin concentrations in mature milk, while only obstetrical parameters were associated adiponectin concentrations in early milk. Our findings support the need for continued work to determine optimal prenatal nutritional strategies to prevent GDM and subsequently to improve infant nutrition.

Substantial evidence supports a role of diet in glucose metabolism, but only a few reports have investigated the impact of diet during pregnancy on risk of gestational diabetes (GDM). Although metabolic hormones have been detected in milk, no studies have investigated the impact of maternal metabolic status assessed during pregnancy on insulin and adiponectin concentrations in human milk. The purpose of this thesis was to investigate the association of maternal macronutrient intakes with metabolic status during pregnancy and its subsequent impact on human milk hormones. Participating women (n=216) underwent a 3-hour oral glucose tolerance test at 30 (95% confidence interval [CI] 25, 33) weeks gestation, recalled their second trimester dietary intake, and donated early (the first week) and mature (3 months postpartum) milk. Higher vegetable and fruit fiber intake was associated with reduced insulin resistance (beta±SE -0.100±0.029, p=0.0008) and increased insulin sensitivity (0.029±0.012, p=0.01) among those with a family history of type 2 diabetes. Lower % carbohydrate and higher % total fat were associated with increased GDM risk (odds ratio 0.60 [95% CI 0.40, 0.90] and 1.61 [1.06, 2.44], respectively). Prenatal metabolic abnormalities including higher pregravid body mass index (beta±SE 0.053±0.014, p=0.0003), in addition to gravid hyperglycemia (0.218±0.087, p=0.01), insulin resistance (0.255±0.047, p<0.0001), lower insulin sensitivity (-0.521±0.108, p<0.0001), and higher serum adiponectin (0.116±0.029, p<0.0001) were associated with higher insulin in mature milk. Obstetrical measures including nulliparity (0.171±0.058, p=0.004), longer duration of gestation (0.546±0.146, p=0.0002), and unscheduled caesarean section (0.387±0.162, p=0.02) were associated with higher adiponectin in early milk. Holder pasteurization, a process recommended by the Human Milk Bank Association of North America before distributing human donor milk, reduced milk adiponectin and insulin concentrations by 32.8% and 46.1%, respectively (both p<0.0001). In conclusion, the distribution of macronutrient intakes during pregnancy was associated with risk for abnormal glucose metabolism later in pregnancy. In addition, maternal prenatal metabolic abnormalities were associated with high insulin concentrations in mature milk, while only obstetrical parameters were associated adiponectin concentrations in early milk. Our findings support the need for continued work to determine optimal prenatal nutritional strategies to prevent GDM and subsequently to improve infant nutrition.

[Truncated abstract] Mothers who deliver prematurely often have a delay in lactogenesis II and subsequent milk supply. Furthermore, due to the inability of their babies to breastfeed immediately after birth, these mothers are 'pump dependent' during both initiation and establishment of lactation. Apparently, there are no evidence based guidelines for the expression regime but some data suggesting that expression regimes for both breasts should be at least five times per day and at least 100 minutes expressing time per day. The project was set out to document the self selected current expression regimes of the preterm mothers from day 10 to 60 postpartum. It defined how various aspects of breast expression, such as frequency and interval, impact on the synthesis and production of milk. In addition, it determined the variations in the composition of preterm mother's milk. The collection of 24hr expression data and milk samples at each expression of each breast, each day, of 25 preterm mothers (<32 gestation age) from the neonatal intensive care unit in King Edward Memorial Hospital, Western Australia on day 10, 15-20, 30, 40, 50 and 60 postpartum showed that during the 'pump dependent' period (day 10 20), the frequency of expression for both breasts was 6, 6-7, 3-9 times per day (median, IQR, range) and total duration with the pump was 115, 80-160, 32-320 minute per day (median, IQR, range). Furthermore, during the 'transition from exclusively expressing to exclusively breastfeeding' period (day 30-60), frequency of expression/breastfeed and total duration of milk removal (both expressing and breastfeeding) for both breasts were 6, 5-7, 1-9 and 135, 75-170, 25-320, respectively (median, IQR, range). ... These nutrients make up the energy content of milk, thus the energy content of milk also varied greatly between mothers. Therefore, milk from individual preterm mothers varies greatly for individual values for fat, total protein, lactose and energy and this should be taken into account when calculating the level of fortification required for individual babies. The results suggest that when fortifying mother's milk, weekly measurement of fat and protein in milk would provide good estimates on which to base fortification requirements. The concentration of sIgA plus lactoferrin formed 32% of the total proteins in breastmilk. However there was large variations in the concentration of sIgA and lactoferrin (median, IQR, range: 0.82, 0.59-1.13, 0.05-2.93g/l and 2.41, 1.52-3.52, 0.04-8.82g/l, respectively) between mothers. Therefore the level of protection provided by these two proteins could differ greatly between babies. Further research on the relationship between the concentration of sIgA and lactoferrin in milk and the onset of infection would indicate the minimum amount of these proteins needed for the babies to benefit from the immune protection provided by their mother's milk. The hourly breast expression method and regression analysis of actual milk yield and interval since previous expression provides information that identifies the potential milk synthesis capacity of the breasts of the mothers and the impact of the interval between expressions on the milk production of the mothers. This information can be applied to individualize the interval between expression regimes to optimise milk production and minimize the demand on the mother. In addition, determining the changes in the milk composition of individual mothers would provide a more precise base to fortify their milk for their preterm babies.

Diss. (sammanfattning) Linköping : Univ., 2002. / Härtill 5 uppsatser.

Made available in DSpace on 2014-12-17T14:46:46Z (GMT). No. of bitstreams: 1 EdualeideJPBN_DISSERT.pdf: 536785 bytes, checksum: 958a6f06670a7767f5dc0cb70479ab35 (MD5) Previous issue date: 2011-03-28 / This is an exploratory and descriptive study that aimed to investigate the actions of professionals in the context of breastfeeding, on the assumption that the actions taken by employees working together to postpartum and newborn are not competing to effect the distribution of pasteurized human milk so that it meets the needs of infants who depend on it. Thus, the study aimed to analyze the actions of medical and nursing staff of the distribution of pasteurized human milk to the newly born. The investigation was developed by action research in a federal hospital, located in the capital the state of Rio Grande do Norte, Brazil, reference assistance to women during pregnancy, childbirth and postpartum high risk in 2010. Study participants were fifty-five professionals chosen from the following inclusion criteria: to act in the NICU or rooming, being a pediatrician and / or neonatologists, nurses and technical nursing. According to the methodology of action research a questionnaire was applied, techniques in focus groups and courses were developed, and, finally, action evaluation. The project was submitted to the Ethics Committee at the Federal University of Rio Grande do Norte and approved with no protocol 448/2009. The problems identified in the responses issued by the social research were grouped into categories according to the similarity between them. The answer to the question of the survey - How is the need for pasteurized human milk for the newborns in neonatal intensive care unit and rooming identified? - Brought subsidies for action planning and implementation of strategies for change in the practice of professionals working in rooming and ICU. Thus, the study has relevance in social care and, when at the local level, will compete for the distribution pasteurized human milk to take effect as best as possible, as recommended by the Ministry of Health. It is also conceived that, in a macro view of society, it could contribute to minimizing the health problem that involves the child population / O leite humano atende ?s necessidades da crian?a com sua composi??o rica em nutrientes, como ?gua, prote?nas, lip?deos, carboidratos, minerais, vitaminas e ferro. Em fun??o disto, ? indicada a amamenta??o exclusiva para a crian?a at? os seis meses de vida. Entretanto, essa pr?tica, al?m de ser biologicamente determinada, ? socialmente condicionada e viabiliz?-la tem sido um grande desafio enfrentado por todos os profissionais que atuam junto ? mulher nessa realidade. O estudo teve o prop?sito de investigar as a??es de profissionais no contexto da amamenta??o, partindo do pressuposto de que as a??es desenvolvidas junto ? pu?rpera e ao rec?m-nascido pouco concorrem para se efetivar a distribui??o do leite humano pasteurizado, em conson?ncia com as necessidades de rec?m-nascidos que dele dependem. Assim, o estudo teve como objetivo geral analisar as a??es de profissionais m?dicos e equipe de enfermagem frente ? distribui??o do leite humano pasteurizado para o rec?m-nascido. Trata-se de um estudo explorat?rio e descritivo, com abordagem qualitativa, desenvolvido ? luz da pesquisaa??o em uma unidade hospitalar federal, situada na capital do estado do Rio Grande do Norte, Brasil, refer?ncia em atendimento ? mulher na gesta??o, no parto e no puerp?rio de alto risco. Participaram da investiga??o cinquenta e cinco profissionais escolhidos a partir dos seguintes crit?rios de inclus?o: atuar em UTI neonatal ou alojamento conjunto, ser m?dico pediatra e/ou neonatologista, enfermeiros e t?cnicos de enfermagem. De acordo com a metodologia da pesquisa-a??o, foi aplicado um question?rio, desenvolvidas t?cnicas de grupo focal, al?m da realiza??o de cursos. Por fim, procedeu-se ? avalia??o das a??es. O projeto foi submetido ao Comit? de ?tica em Pesquisa da Universidade Federal do Rio Grande do Norte e aprovado com Protocolo de n. 448/2009. Os problemas detectados nas respostas emitidas pelos atores sociais da pesquisa foram agrupados em categorias, de acordo com a similaridade existente entre si. A resposta ao questionamento da pesquisa - Como ? identificada a necessidade do leite humano pasteurizado para o rec?m-nascido em alojamento conjunto e UTI neonatal?- trouxe subs?dios para o planejamento de a??es e a implementa??o de estrat?gias de mudan?as na pr?tica dos profissionais atuantes no Alojamento Conjunto e na UTI Neonatal. Os participantes, embora tenham demonstrado conhecimento em rela??o ao funcionamento do BLH, evidenciaram desconhecer os problemas existentes no setor, no que diz respeito ? capta??o do leite humano. Tratando-se das a??es que os profissionais desenvolvem no processo da distribui??o, observou-se que estes revelaram conhecer, em sua maioria, as indica??es do RN em receber complemento em sua dieta, ressaltando-se que n?o foi poss?vel identificar no question?rio e nos grupos focais a preocupa??o em realizar o exame cl?nico na m?e antes da prescri??o. Considera-se que a equipe necessita apoiar efetivamente a pu?rpera na amamenta??o, com vistas a esclarecer d?vidas ou complica??es que possam surgir, prevenindo, inclusive, contra o desmame precoce. Assim sendo, o estudo apresenta relev?ncia no ?mbito assistencial e social e, em n?vel local, concorrer? para que a distribui??o do leite humano pasteurizado seja efetivada da melhor forma poss?vel, como preconizado pelo Minist?rio da Sa?de. Concebe-se, ainda, que, numa vis?o macro da sociedade, o referido estudo poder? contribuir para uma minimiza??o da problem?tica de sa?de que envolve a popula??o infantil

Objetivo: Verificar a associação entre os níveis de leptina do leite materno maduro e os escores de dobras cutâneas aos três e seis meses de vida de crianças expostas a diferentes ambientes intrauterinos. Metodologia: Trata-se de um estudo observacional longitudinal, para o qual foram recrutados pares de mães e crianças residentes em Porto Alegre. Foram excluídas puérperas com teste positivo para HIV, doenças hipertensivas específicas da gravidez, hipertensão arterial sistêmica crônica ou gestacional, crianças gemelares, prematuras, com doenças congênitas ou necessidade de internação hospitalar. Os pares foram divididos, conforme a exposição ao ambiente intrauterino, em grupo Diabetes Mellitus (DM), Tabaco, Pequeno para Idade Gestacional (PIG) e Controle. As entrevistas foram realizadas um, três e seis meses após o parto, e foram coletados dados antropométricos da mãe e da criança, além de informações sobre a alimentação desta. Na entrevista de um mês de vida, foi coletada amostra de leite materno, analisada para quantificação da leptina. Quanto aos procedimentos estatísticos, foi considerado o nível de significância de 5%. Para comparação entre variáveis categóricas e contínuas, quando paramétricas, foi utilizado o teste ANOVA com post hoc de Tukey, e para variáveis não paramétricas, foi utilizado o teste de Kruskal-Wallis com post hoc de Dunn. Na comparação entre variáveis categóricas, foi utilizado o teste de qui-quadrado de Pearson ou Exato de Fisher. Ao analisar variáveis contínuas, foi aplicada a correlação de Spearman. Resultados: Foram analisados os dados de 127 pares mãe-criança, sendo 18 no grupo DM, 34 no grupo Tabaco, 16 no grupo PIG e 59 no grupo Controle. A quantidade de leptina no leite materno não diferiu entre os grupos (p = 0,59). Houve correlação significativa positiva entre a quantidade de leptina do leite e o Índice de Massa Corporal (IMC) materno, a dobra cutânea tricipital (DCT) e a dobre cutânea subescapular (DCSE) maternas (p < 0,001). Sem considerar o tipo de alimentação, não houve correlação entre a leptina e as variáveis antropométricas da criança (p > 0,05). Dentre as crianças amamentadas exclusivamente aos três meses de vida, a leptina do leite materno correlacionou-se negativamente com o escore Z da DCT aos três meses (r = -0,447; p = 0,032) e seis meses (r = -0,499; p = 0,015), além do peso aos três (r = -0,408; p = 0,043) e seis meses de vida (r = -0,443; p= 0,034). Também houve correlação negativa com o ganho de peso do nascimento aos seis meses (r = -0,472; p = 0,023) e de um a três meses (r = -0,396; p = 0,050). Conclusão: A leptina, oferecida através do aleitamento materno exclusivo, atua na regulação do balanço energético corporal da criança, influenciando precocemente variáveis antropométricas. / Objective: Assess the association between mature breast milk leptin and skinfold thickness Z scores at 3 and 6 months of life of infant exposed to different intrauterine environments. Methods: This is a longitudinal study, for which mother and newborn pairs were recruited from 24-48 hours postpartum in three public hospitals in Porto Alegre city, Brazil. Exclusion criteria were mothers who test positive for HIV, with hypertensive disorders, newborn from twin gestation, preterm (gestational age <37 weeks), with malformations at birth, or requiring hospitalization. Pairs were divided as exposure to intrauterine environment in Diabetes Mellitus (DM), Tobacco, Small for Gestational Age (SGA) and Control group. Interviews occurred in postpartum, 1, 3 and 6 months of infant's life, where mother and infant anthropometric data were collected and information about infant’s complementary food was asked. The breast milk sample was collected in the 1 month interview. About statistical procedures, a significance level of 5% was considered. To compare categorical and continuous variables, was used the ANOVA test with Tukey post hoc, when parametric. For nonparametric variables, was used Kruskal -Wallis test with Dunn post hoc. When comparing categorical variables, was used the chi-square of Pearson or Fisher's exact test. To analyze continuous variables, the Spearman correlation was applied. Results: Data of 127 pairs were analyzed, 18 in DM group, 34 Tobacco, 16 SGA and 59 Control. Value of leptin in breast milk did not differ between groups (p = 0.59). There was a significant positive correlation between leptin milk and maternal anthropometric variables in the overall sample (p < 0.001) and between the groups (p< 0.05). Without considering infant's complementary feeding, there was no correlation between leptin and infant anthropometric variables (p > 0.05). However, among the infants exclusively breastfed at 3 months, leptin significantly negative correlated with Z score of triceps skinfold thickness at 3 (r = -0.447; p = 0.032) and 6 months (r = -0.499; p=0.015), infant weight at 3 (r = -0.408; p = 0.043) and 6 months (r = -0.443; p = 0.034), weight gain from birth to 6 months (r = -0.472; p = 0.023) and from 1 to 3 months (r = -0.396; p = 0.050) in the overall sample. Conclusion: Among the infants exclusively breastfed, leptin in mature milk acts in body energy balance and may impact early infant anthropometric variables.

abstract: Exclusivity and duration of breastfeeding and the provision of human milk in the United States is suboptimal. In the absence of adequate banked donor human milk for distribution to all infants in need, many families choose to engage in the practice of Private Arrangement Milk Sharing (PAMS), partially facilitated through social media, to procure human milk for their infants. Evidence regarding the participant and infant characteristics and risk abatement practices is incomplete. This dissertation describes and explores the characteristics of recipient participants and infants, family constellation, donor screening practices, and related risk abatement strategies. Data was collected via on-line survey as a sub-group of a larger data set including donor participants and international participants. Binary logistic regression modeling of factors that contribute to consistent screening and risk abatement practices and important antecedents to engaging in PAMS was conducted. Results are contextualized within a tailored socioecological framework of factors affecting infant feeding practices. Tailoring was accomplished via qualitative descriptive analysis of participant responses applied to an existing breastfeeding framework. Participants in this sample were predominantly white, married, with a mean age of 32.9 years, with at least some college education and above median income. Risk abatement and screening practices were influenced by support of a healthcare provider during decision-making, college education, infant age and health status, having lactation support, birth type and birth attendant, and the duration and sources sought for learning about milk sharing. / Dissertation/Thesis / Doctoral Dissertation Nursing and Healthcare Innovation 2016

Submitted by Ronildo Prado (producaointelectual.bco@ufscar.br) on 2017-11-01T13:44:12Z No. of bitstreams: 1 DissCO.pdf: 1854670 bytes, checksum: 009fc8014d6d3482f98fadb1afa9d6a9 (MD5) / Approved for entry into archive by Ronildo Prado (producaointelectual.bco@ufscar.br) on 2017-11-01T13:44:25Z (GMT) No. of bitstreams: 1 DissCO.pdf: 1854670 bytes, checksum: 009fc8014d6d3482f98fadb1afa9d6a9 (MD5) / Approved for entry into archive by Ronildo Prado (producaointelectual.bco@ufscar.br) on 2017-11-01T13:44:35Z (GMT) No. of bitstreams: 1 DissCO.pdf: 1854670 bytes, checksum: 009fc8014d6d3482f98fadb1afa9d6a9 (MD5) / Made available in DSpace on 2017-11-01T13:44:45Z (GMT). No. of bitstreams: 1 DissCO.pdf: 1854670 bytes, checksum: 009fc8014d6d3482f98fadb1afa9d6a9 (MD5) Previous issue date: 2016-08-16 / Não recebi financiamento / The Human Milk is considered the most complete food for the newborn, to promote the collection and distribution with certified quality, the Ministry of Health in the public policy scenario promotes breastfeeding and expansion of the Brazilian Network of Human Milk Bank which was created to ensure certification and the quality of human milk from collection to distribution, it is responsible for the selection, physicochemical and microbiological evaluation, providing an innocuous product and respecting their role as food. To contribute to the scientific evidence this study was to evaluate the microbiological quality of samples of human milk from milk bank in São Carlos / SP, through analysis and quantification in petri dishes by solid culture of mesophilic, psychrophilic, thermophilic, Staphylococcus spp. filamentous fungi and leveduriform and most probable number (MPN) of total and fecal coliforms through presumptive test and confirmatory, as well as verification of physical-chemical parameters of pH, acidity ° Dornic and energy content (K) correlating the variables in the microbiological quality by descriptive statistical analysis and linear correlation Pearson. Of the 29 analyzed samples and unfit for consumption, revealed the presence of 17.24% of psychrophilic, 27.59% of thermophiles, 55.17% of mesophilic, 41.38% of filamentous fungi and leveduriform absence of Staphylococcus spp, for coliform group was the presence of 82.76% presumptive phase and 54.16% for total coliforms and 33.33% for fecal coliforms in the confirmatory phase, pH values and K showed no variation, as for acidity was microbial growth between 3 ° to 15 ° Dornic, the correlation of physical and chemical variables was detected. The results presented in analyzes suggest that was deficient in good manufacturing practices, impairing the microbiological quality of human milk. / O Leite Humano é considerado o alimento mais completo para o recémnascido, para promover a coleta e distribuição com qualidade certificada, o Ministério da Saúde no cenário das políticas públicas promove o aleitamento materno e expansão da Rede Brasileira de Banco de Leite Humano o qual foi criado para garantir a certificação e a qualidade do Leite Humano Ordenhado, desde a coleta até a distribuição, sendo sua responsabilidade a seleção, avaliação físico-química e microbiológica, fornecendo um produto inócuo e respeitando sua função como alimento. Para contribuir com as evidencias cientificas o presente estudo teve como objetivo avaliar a qualidade microbiológica de amostras de leite humano proveniente de Banco de Leite no município de São Carlos/SP, por intermédio de análise e da quantificação em placas de petri por meio de cultura sólida dos microrganismos mesófilos, psicrófilos, termófilos, Staphylococcus spp. fungos filamentosos e leveduriformes e número mais provável (NMP) de coliformes totais e termotolerantes por meio de teste presuntivo e confirmativo, além da verificação do parâmetro físico químico do pH, acidez ° Dornic e do teor energético (K) correlacionando as variáveis na qualidade microbiológica por meio de análise estatística descritiva e a correlação linear Pearson. Das 29 amostras analisadas e impróprias para consumo, evidenciou a presença de 17,24% de psicrófilos, 27,59% de termófilos, 55,17% de mesófilos, 41,38% de fungos filamentosos leveduriformes e ausência de Staphylococcus spp, para o grupo de coliformes houve a presença de 82,76% fase presuntiva e 54,16% para coliformes totais e 33,33% para coliformes termotolerantes na fase confirmativa, os valores de pH e K não apresentaram variação, já para acidez houve o crescimento microbiano entre 3° a 15° Dornic, não foi detectada a correlação das variáveis físico-química. Os resultados apresentados nas análises sugerem que o houve deficiência nas boas práticas de manipulação, prejudicando a qualidade microbiológica de leite humano.