A detecção de gestos é uma etapa importante em aplicações de interação humanocomputador. Se a mão do usuário é detectada com precisão, tanto a análise quanto o reconhecimento do gesto de mão se tornam mais simples e confiáveis. Neste trabalho, descrevemos um novo método para detecção de pele humana, destinada a ser empregada como uma etapa de pré-processamento para segmentação de gestos de mão em sistemas que visam o seu reconhecimento. Primeiramente, treinamos os modelos de cor e textura de pele (material a ser identificado) a partir de um conjunto de treinamento formado por imagens de pele. Nessa etapa, construímos um modelo de mistura de Gaussianas (GMM), para determinar os tons de cor da pele e um dicionário de textons, para textura de pele. Em seguida, introduzimos um estratégia de fusão estocástica de regiões de texturas, para determinar todos os segmentos de diferentes materiais presentes na imagem (cada um associado a uma textura). Tendo obtido todas as regiões, cada segmento encontrado é classificado com base nos modelos de cor de pele (GMM) e textura de pele (dicionário de textons). Para testar o desempenho do algoritmo desenvolvido realizamos experimentos com o conjunto de imagens SDC, projetado especialmente para esse tipo de avaliação (detecção de pele humana). Comparado com outras técnicas do estado-daarte em segmentação de pele humana disponíveis na literatura, os resultados obtidos em nossos experimentos mostram que a abordagem aqui proposta é resistente às variações de cor e iluminação decorrentes de diferentes tons de pele (etnia do usuário), assim como de mudanças de pose da mão, mantendo sua capacidade de discriminar pele humana de outros materiais altamente texturizados presentes na imagem. / Gesture detection is an important task in human-computer interaction applications. If the hand of the user is precisely detected, both analysis and recognition of hand gesture become more simple and reliable. This work describes a new method for human skin detection, used as a pre-processing stage for hand gesture segmentation in recognition systems. First, we obtain the models of color and texture of human skin (material to be identified) from a training set consisting of skin images. At this stage, we build a Gaussian mixture model (GMM) for identifying skin color tones and a dictionary of textons for skin texture. Then, we introduce a stochastic region merging strategy, to determine all segments of different materials present in the image (each associated with a texture). Once the texture regions are obtained, each segment is classified based on skin color (GMM) and skin texture (dictionary of textons) model. To verify the performance of the developed algorithm, we perform experiments on the SDC database, specially designed for this kind of evaluation (human skin detection). Also, compared with other state-ofthe- art skin segmentation techniques, the results obtained in our experiments show that the proposed approach is robust to color and illumination variations arising from different skin tones (ethnicity of the user) as well as changes of pose, while keeping its ability for discriminating human skin from other highly textured background materials.

D’un point de vue mécanique, la peau est une structure multicouche complexeayant des propriétés viscoélastique, non-linéaire, quasi-incompressible, anisotrope eten état de précontrainte. Le travail présenté dans cette thèse associe expérimentation,modélisation et identification numérique et se distingue en particulier parl’utilisation d’un dispositif d’extensométrie développé au laboratoire et adapté à desmesures in vivo non invasives. Des tests ex vivo ont cependant été réalisés égalementà titre de comparaison et de validation. Une attention particulière a été portée à latension cutanée initiale (ou naturelle). Les essais in vivo ont permis d’obtenir desréponses force – déplacement sous différentes configurations angulaires, d’intensitéet pour diverses localisations corporelles. Les essais ex vivo ont quant à eux permisd’estimer l’état de contrainte initiale par la mesure des forces nécessaires à la remiseen tension d’explants. Ces différents essais expérimentaux ont été modélisés en utilisantdeux lois de comportement : la loi d’Ogden du premier ordre permettant dedécrire un comportement hyperélastique isotrope et la loi d’Holzapfel-Gasser-Ogden(HGO) décrivant un comportement hyper élastique anisotrope. Cette dernière a étéimplémentée sous l’interface utilisateur du logiciel ANSYS. Les paramètres caractéristiquesdes zones cutanées testées ont été identifiés par méthode inverse. L’influencede la compressibilité de la peau sur son comportement mécanique est mise en évidence.Au final, les travaux de cette thèse ont été appliqués au lambeau d’avancementde type V-Y qui est une technique de suture pratiquée pour combler les pertes desubstance.229 / From a mechanical point of view, the human skin is a complex multilayerstructure with viscoelastic, non-linear and anisotropic properties and a pre-stressstate. The work presented in this thesis combines experimentation, modeling andnumerical identification and distinguishes especially by the use of an extensometerdevice developed in the laboratory and suitable for non-invasive in vivo measurements.Ex vivo tests were however also performed for comparison and validation.Particular attention was paid to the initial skin tension. in vivo tests allowed theobtaining of load – displacement responses for different angular configurations, intensitiesand body locations. ex vivo tests in turn allowed the estimation of the stateof initial stress by measuring the forces necessary for the re-tension of the explants.These different experimental tests were modeled using two constitutive laws : thefirst order Ogden law allowing the description of an isotropic hyperelastic behavior,and the Holzapfel-Gasser-Ogden’ law (HGO) allowing the description of an anisotropichyperelastic behavior. The latter was implemented in the user interface ofANSYS software. The characteristics parameters of the skin areas tested were identifiedby the reverse method. The influence of the compressibility of the skin on itsmechanical behavior is highlighted. Finally, the work of this thesis were applied toan advancement flap of V-Y type.

The ultraviolet (UV) spectrum has been known to cause damage to skin in varying degrees. UVB radiation (290-320 nm) in particular, has been proven to be highly mutagenic and carcinogenic in many animal experiments compared to either UVA or UVC. The alarming rate of increase in skin cancer incidence has prompted many investigators to pursue other alternatives to sunscreens including changes in lifestyle habits and dietary consumption in order to boost our efforts in tackling this widespread disease. Previous studies employing confocal reflectance, MTT assay and histology suggest that milk phospholipids may possess protective properties against UVB-mediated damage but the molecular mechanism for this effect remains unclear. This study aims to evaluate changes in cell morphology, apoptosis and p21 expression in tissue engineered epidermis to increase our understanding of the mechanisms behind the potential protective effects of milk phospholipids against UV-induced photodamage. Human skin tissue equivalents were incubated in either 1% milk phospholipid solution or maintenance media then exposed to 120 mJ/cm2 dose of 300 nm UVB after 24 hours. The upregulation of p21 protein in response to DNA damage was measured with Western blot and immunofluorescence microscopy and markers for apoptosis and hyperplasia were examined 24 hours after irradiation. Results revealed that p21 levels and the amount of apoptotic markers such as fragmented DNA and nuclear condensation were significantly reduced in UV-exposed tissues pre-incubated with milk phospholipids compared to levels seen in both the positive control and UV-exposed skin tissue not incubated with milk phospholipids. This decrease in p21 expression may imply a reduction in DNA damage 24 hours after UV exposure or a decrease in acquired photodamage at the outset. Milk phospholipid incubation however, induced an increase in epidermal thickening with or without UV exposure, which may imply induction of a protective mechanism to enhance the barrier properties of skin.

TGF-β isoforms are important signalling molecules in wound repair in the skin. Transforming growth factor β3 (TGF-β3) has been implicated in scarless healing. In both animal and human models the application of exogenous TGF-β3 causes a reduction in the inflammatory response and improves the architecture of the neodermis. Research into the influence of TGF-β on scarring has tended to focus on fibroblasts. However, keratinocytes play a major role in scarring both indirectly, as a result of their influence over the behaviour of fibroblasts and also by directly influencing wound contraction. Thus, experiments were carried out to investigate the influence of TGF-β3 on the behaviours of a keratinocyte cell line (HaCaT). Incubation with TGF-β3 increased cell spreading and appeared to reduce cell-surface contacts indicated by both SPR imaging and a detachment assay. TGF-β3 also caused a decreased cell alignment response to microcontact printed protein patterns, in part due to the deposition of laminin which is associated with the TGF-β induced cell migration. There is evidence that TGF-β isoforms differentially influence the outcome of wound healing. Similar to the results produce following addition of exogenous TGF-β3, the neutralisation of TGF-β1 and 2 has been shown to reduce scar formation in the adult wounds. During reepithelialisation keratinocytes experience a dynamic environment. Both extracellular matrix proteins and growth factors influence the progression of wound repair which includes both cell migration and proliferation. Few studies have examined collective cell behaviour in response to TGF-β isoforms and ECM coated substrates. Thus both wound closure and cell proliferation assays were conducted for different ECM proteins fibronectin, laminin and collagen type I and for TGF-β1, 2 and 3. Rates of wound closure were significantly reduced on laminin coated substrates while cell proliferation rates were increased. TGF-β2 and 3 induced significant increases in wound closure rates. This appeared to correspond with an increase in the number of cells independently migrating out from the wound margins. Only TGF-β3 caused a significant decrease in cell proliferation over a 4 day period. Laminin332 deposition is central to the reepithelialisation process and is known to be induced in response to TGF-β. Thus experiments were carried out to investigate HaCaT cell laminin332 deposition in response to TGF-β1, 2 and 3. Both an immunofluorescence staining technique and an ELISA based semi-quantification method was used. Following 4 day incubation all TGF-β isoforms significantly increased laminin332 deposition; however TGF-β2 and 3 caused the most significant increases. Integrin receptors enable cell-matrix interactions during wound repair. TGF-β is known to influence the expression of integrin subunits. Thus, experiments were carried out to compare the influence of each TGF-β isoform on the expression of subunits α3, α2, α5, β1 and β4. All TGF-β isoforms significantly increased all subunit expression. TGF-β3 caused the most significant increase in β4 and both TGF-β2 and 3 caused the most significant increase in α2. While there were differences in cell responses to each isoforms, TGF-β3 did not stand out from the other two isoforms. Interestingly, TGF-β2 shared more similarities with TGF-β3 than it did with TGF-β1, in its role in enhancing wound closure and LN332 deposition. These comparative studies have shown that differences exist in the way TGF-β isoforms influence HaCaT cell behaviour, namely migration, laminin deposition and integrin expression. / EPSRC and DTA grant

No / Nonsegmental vitiligo (NSV) is characterized by loss of inherited skin color. The cause of the disease is still unknown despite accumulating in vivo and in vitro evidence of massive epidermal oxidative stress via H2O2 and peroxynitrite (ONOO−) in affected individuals. The most favored hypothesis is based on autoimmune mechanisms. Strictly segmental vitiligo (SSV) with dermatomal distribution is a rare entity, often associated with stable outcome. Recently, it was documented that this form can be associated with NSV (mixed vitiligo). We here asked the question whether ROS and possibly ONOO− could be players in the pathogenesis of SSV. Our in situ results demonstrate for the first time epidermal biopterin accumulation together with significantly decreased epidermal catalase, thioredoxin/thioreoxin reductase, and MSRA/MSRB expression. Moreover, we show epidermal ONOO− accumulation. In vivo FT-Raman spectroscopy reveals the presence of H2O2, methionine sulfoxide, and tryptophan metabolites; i.e., N-formylkynurenine and kynurenine, implying Fenton chemistry in the cascade (n=10). Validation of the basic data stems from successful repigmentation of skin and eyelashes in affected individuals, regardless of SSV or segmental vitiligo in association with NSV after reduction of epidermal H2O2 (n=5). Taken together, our contribution strongly supports H2O2/ONOO-mediated stress in the pathogenesis of SSV. Our findings offer new treatment intervention for lost skin and hair color.—Schallreuter, K. U., Salem, M. A. E. L., Holtz, S., Panske, A. Basic evidence for epidermal H2O2/ONOO−-mediated oxidation/nitration in segmental vitiligo is supported by repigmentation of skin and eyelashes after reduction of epidermal H2O2 with topical NB-UVB-activated pseudocatalase PC-KUS.

Yes / Creation of functional skin substitutes within a clinically acceptable time window is essential for timely repair and management of large wounds such as extensive burns. The aim of this study was to investigate the possibility of fabricating skin substitutes via a bottom-up nanofiber-enabled cell assembly approach and using such substitutes for full-thickness wound repair in nude mice. Following a layer-by-layer (L-b-L) manner, human primary skin cells (fibroblasts and keratinocytes) were rapidly assembled together with electrospun polycaprolactone (PCL)/collagen (3:1 w/w, 8% w/v) nanofibers into 3D constructs, in which fibroblasts and keratinocytes were located in the bottom and upper portion respectively. Following culture, the constructs developed into a skin-like structure with expression of basal keratinocyte markers and deposition of new matrix while exhibited good mechanical strength (as high as 4.0 MPa by 14 days). Treatment of the full-thickness wounds created on the back of nude mice with various grafts (acellular nanofiber meshes, dermal substitutes, skin substitutes and autografts) revealed that 14-day-cultured skin substitutes facilitated a rapid wound closure with complete epithelialization comparable to autografts. Taken together, skin-like substitutes can be formed by L-b-L assembling human skin cells and biomimetic nanofibers and they are effective to heal acute full-thickness wounds in nude mice.

Erythema is a hallmark skin response to excessive ultraviolet radiation (UVR) and is associated with cutaneous inflammation. Both are mediated by inflammatory mediators including nitric oxide (NO), prostaglandin E2 (PGE2) and chemoattractants such as 12-hydroxyeicosatetraenoic acid (12-HETE) leading to vasodilation and increased leukocyte infiltration. The erythematous response is more pronounced in individuals with low basal melanin levels or who fail to respond to UVR with a robust up-regulation of melanogenesis. While melanin production is a key function of melanocytes, these cells can also produce NO and PGE2, and are located in close proximity to the dermal vasculature. It has been hypothesized that melanocytes with poor melanogenic capacity may participate in the inflammatory response to UVR. The aim of this project was to investigate the inflammatory response in the skin of individuals with either skin phototype (SPT) 1 or 4 to UVR. Sixteen normal healthy individuals were selected for study (8 SPT-1 & 8 SPT-4). Buttock skin was investigated by immunohistochemistry for leukocyte subtypes, eicosanoid producing enzymes and NO synthases under basal and UVR-stimulated conditions. In addition primary cultures of epidermal melanocytes (EM) were established from 16 individuals (8 SPT-1 & 8 SPT-4) and assessed for the presence of eicosanoid-producing enzymes, melanogenic enzymes and NO synthases, by immunocytochemistry, Polymerase Chain Reaction and Western Blotting and for the production of the main pro-inflammatory eicosanoid PGE2 by ELISA and Mass Spectrometry. Moreover, the fatty acid composition of cultured melanocytes was assessed by Gas Chromatography. Results showed that individuals with SPT-1 had significantly greater neutrophil infiltration into the epidermis than those with SPT-4 at 24 hrs post-UVR. Moreover, CD3+ lymphocyte infiltration into the dermis was significantly greater in individuals with SPT-4 than those with SPT-1 at 24 and 72 hrs post-UVR. NOS-1, NOS-3, 12-LOX and COX-2 expression were significantly increased in SPT-1 skin, while NOS-2 and 15-LOX were significantly increased in SPT-4 skin. As 12-LOX and COX-2 products are chemoattractive (for neutrophils) and pro-inflammatory respectively these data could explain the greater observed neutrophil infiltration in SPT-1. The 15-LOX product (15-HETE) is anti-inflammatory and may suggest that 15-LOX up-regulation in SPT-4 skin may aid resolution of the sunburn response, which in part may be mediated by CD3+ lymphocytes and a class-switch in eicosanoid production from COX to LOX products. Melanocyte primary cultures surprisingly showed that SPT was not correlated with melanin content or melanogenic enzyme expression/activity suggesting that all melanocytes in vitro contained the necessary cellular machinery to produce melanin. This finding may reflect also their equal treatment under these enriched culture conditions, which may or may not be available to these cells in situ. Moreover, all melanocytes expressed the necessary machinery (PLA2, COX-1, cPGES) to produce PGE2. However, only some cultures did so at baseline and in response to UVR, and this was not correlated with SPT. A positive correlation was found however between expression level of dopachrome tautomerase (DCT) and protection against PGE2 production in response to UVR, which may suggest a novel role for DCT unrelated to melanogenesis. In summary this research project has generated data that highlights differences between the skin of individuals with SPT-1 and those with SPT-4, and may provide evidence that the keratinocyte partner contributes significantly to the SPT-associated response. This research may also suggest DCT as a novel therapeutic target to protect EM from participation in the UVR-associated inflammatory response in skin.

Bone morphogenetic protein (BMP) signalling plays key roles in skin development and also possesses a potent anti-tumour activity in postnatal skin. To study mechanisms of the tumour-suppressive role of BMPs in the skin, a transgenic (TG) mouse model was utilized, in which a transgenic expression of the BMP antagonist Noggin was targeted to the epidermis and hair follicles (HFs) via Keratin 14 promoter. K14-Noggin mice developed spontaneous HF-derived tumours, which resembled human trichofolliculoma. Initiation of the tumours was associated with a marked increase in cell proliferation and an expansion of the hair follicle stem/early progenitor cells. In addition, the TG mice showed hyperplastic changes in the sebaceous glands and the interfollicular epidermis. The epidermal hyperplasia was associated with an increase in the susceptibility to chemically-induced carcinogenesis and earlier malignant transformation of chemically-induced papillomas. Global gene expression profiling revealed that development of the trichofolliculomas was associated with an increase in the expression of the components of several pro-oncogenic signalling pathways (Wnt, Shh, PDGF, Ras, etc.). Specifically, expression of the Wnt ligands and (β-catenin/Lef1) markedly increased at the initiation stage of tumour formation. In contrast, expression of components of the Shh pathway was markedly increased in the fully developed tumours, compared to the tumour placodes. Pharmacological treatment of the TG mice with the Wnt and Shh antagonists resulted in the stage-dependent inhibition of the tumour initiation and progression, respectively. Further studies revealed that BMP signalling antagonizes the activity of the Wnt and Shh pathways via distinct mechanisms, which include direct regulation of the expression of the tumour suppressor Wnt inhibitory factor 1 (Wif1) and indirect effects on the Shh expression. Thus, tumour suppressor activity of the BMPs in skin epithelium depends on the local concentrations of Noggin and is mediated, at least in part, via stage-dependent antagonizing of the Wnt and Shh signalling pathways.

O reconhecimento de língua de sinais é uma importante área de pesquisa que tem como objetivo atenuar os obstáculos impostos no dia a dia das pessoas surdas e/ou com deficiência auditiva e aumentar a integração destas pessoas na sociedade majoritariamente ouvinte em que vivemos. Baseado nisso, esta dissertação de mestrado propõe o desenvolvimento de um sistema de informação para o reconhecimento automático de Língua Brasileira de Sinais (LIBRAS), que tem como objetivo simplificar a comunicação entre surdos conversando em LIBRAS e ouvintes que não conheçam esta língua de sinais. O reconhecimento é realizado por meio do processamento de sequências de imagens digitais (vídeos) de pessoas se comunicando em LIBRAS, sem o uso de luvas coloridas e/ou luvas de dados e sensores ou a exigência de gravações de alta qualidade em laboratórios com ambientes controlados, focando em sinais que utilizam apenas as mãos. Dada a grande dificuldade de criação de um sistema com este propósito, foi utilizada uma abordagem para o seu desenvolvimento por meio da divisão em etapas. Considera-se que todas as etapas do sistema proposto são contribuições para trabalhos futuros da área de reconhecimento de sinais, além de poderem contribuir para outros tipos de trabalhos que envolvam processamento de imagens, segmentação de pele humana, rastreamento de objetos, entre outros. Para atingir o objetivo proposto foram desenvolvidas uma ferramenta para segmentar sequências de imagens relacionadas à LIBRAS e uma ferramenta para identificar sinais dinâmicos nas sequências de imagens relacionadas à LIBRAS e traduzi-los para o português. Além disso, também foi construído um banco de imagens de 30 palavras básicas escolhidas por uma especialista em LIBRAS, sem a utilização de luvas coloridas, laboratórios com ambientes controlados e/ou imposição de exigências na vestimenta dos indivíduos que executaram os sinais. O segmentador implementado e utilizado neste trabalho atingiu uma taxa média de acurácia de 99,02% e um índice overlap de 0,61, a partir de um conjunto de 180 frames pré-processados extraídos de 18 vídeos gravados para a construção do banco de imagens. O algoritmo foi capaz de segmentar pouco mais de 70% das amostras. Quanto à acurácia para o reconhecimento das palavras, o sistema proposto atingiu 100% de acerto para reconhecer as 422 amostras de palavras do banco de imagens construído, as quais foram segmentadas a partir da combinação da técnica de distância de edição e um esquema de votação com um classificador binário para realizar o reconhecimento, atingindo assim, o objetivo proposto neste trabalho com êxito. / The recognition of sign language is an important research area that aims to mitigate the obstacles in the daily lives of people who are deaf and/or hard of hearing and increase their integration in the majority hearing society in which we live. Based on this, this dissertation proposes the development of an information system for automatic recognition of Brazilian Sign Language (BSL), which aims to simplify the communication between deaf talking in BSL and listeners who do not know this sign language. The recognition is accomplished through the processing of digital image sequences (videos) of people communicating in BSL without the use of colored gloves and/or data gloves and sensors or the requirement of high quality recordings in laboratories with controlled environments focusing on signals using only the hands. Given the great difficulty of setting up a system for this purpose, an approach divided in several stages was used. It considers that all stages of the proposed system are contributions for future works of sign recognition area, and can contribute to other types of works involving image processing, human skin segmentation, object tracking, among others. To achieve this purpose we developed a tool to segment sequences of images related to BSL and a tool for identifying dynamic signals in the sequences of images related to the BSL and translate them into portuguese. Moreover, it was also built an image bank of 30 basic words chosen by a BSL expert without the use of colored gloves, laboratory-controlled environments and/or making of the dress of individuals who performed the signs. The segmentation algorithm implemented and used in this study had a average accuracy rate of 99.02% and an overlap of 0.61, from a set of 180 preprocessed frames extracted from 18 videos recorded for the construction of database. The segmentation algorithm was able to target more than 70% of the samples. Regarding the accuracy for recognizing words, the proposed system reached 100% accuracy to recognize the 422 samples from the database constructed (the ones that were segmented), using a combination of the edit distance technique and a voting scheme with a binary classifier to carry out the recognition, thus reaching the purpose proposed in this work successfully.

O presente trabalho teve o objetivo de desenvolver uma formulação tópica contendo os filtros solares benzofenona-3 e avobenzona microencapsulados em associação com filtro solar não encapsulado octocrileno e nanoparticulas lipídicas sólidas contendo rutina (formulação completa) e avaliar a eficácia fotoquimiopreventiva dessa formulação usando biópsias de pele humana e pele reconstruída in vitro. Microparticulas lipídicas sólidas contendo grandes quantidades de filtros solares, benzofenona-3 e avobenzona foram obtidas pela técnica do spray congealing com propriedades adequadas para aplicação tópica. Além disso, o processo de microencapsulação foi capaz de diminuir a penetração de benzofenona-3 na pele, aumentar a estabilidade da avobenzona frente à radiação ultravioleta A e a capacidade fotoprotetora desses filtros microencapsulados em formulações tópicas quando expostos a radiação ultravioleta. Nanopartículas lipídicas sólidas contendo o flavonóide rutina foram produzidas pelo processo de homogeneização a alta pressão e suas condições foram otimizadas pelo método da desejabilidade rendendo nanopartículas lipídicas sólidas com tamanho médio de 74,22 ±2,77 nm, índice de polispersividade de 0,161±0,03 e eficiência de encapsulação de 98,90 ±0,25 %. Em adição, as nanopartículas mostraram serem capazes de proteger a viabilidade celular de fibroblastos de ratos L929 irradiados com radiação ultravioleta A e B. Para a eficácia fotoquimiopreventiva a formulação completa foi capaz de evitar/diminuir a formação de células apoptóticas, caspase-3, dímeros de ciclobutanodipirimidina, metaloproteinases e peroxidação lipídica em pele humana e pele reconstruída expostos a UVB. O processo tecnológico de microencapsulação e nanoencapsulação dos ativos avaliados mostrou ser eficaz, não comprometendo as propriedades de fotoproteção dos filtros solares e rutina, apresentando resultados similares ou melhores do que as formulações contendo os ativos na forma livre. Portanto, o desenvolvimento de formulações contendo ativos microencapsulados e nanoencapsulados é uma alternativa interessante para o emprego em produtos comerciais para proteção solar, por diminuir as características indesejáveis como penetração e instabilidade, melhorando as propriedades fotoprotetoras e evitando a necessidade de desenvolver novos compostos com propriedades fotoprotetoras. / This study aimed the pharmaceutical development of a topical formulation containing an association of microencapsulated sunscreens benzophenone-3 and avobenzone, free sunscreen octocrylene and rutin flavonol solid lipid nanoparticles (complete formulation). This formulation was assessed for photochemoprotective ability using human skin obtained surgically and reconstructed human skin. Solid lipid microparticles containing large amounts of sunscreens benzophenone-3 and avobenzone were obtained by the spray congealing technique under conditions that allowed the manufacture of microparticles with suitable properties for topical application. The microencapsulation conditions were also able to reduce the penetration of benzophenone-3 through the skin, enhanced the stability of avobenzone against the ultraviolet radiation (UVR) and increased the photoprotective ability of both filters in topical formulations exposed to UVR. Solid lipid nanoparticles containing rutin were produced by the high pressure homogenization process whose conditions were optimized using the desirability method, yielding nanoparticles with size of 74.22 ± 2.77 nm, polispersivity index of 0.161 ± 0.03 and encapsulation efficiency of 98.90 ± 0.25%. In addition, the nanoparticles were able to avoid the death of L929 mice fibroblasts exposed to UVR A and B. For the photochemopreventive ability studies, the complete formulation was able to reduce/avoid the induction of apoptotic cells, caspase-3, CPDs, metalloproteinases and lipid peroxides in human skin obtained surgically and reconstructed human skin in vitro exposed to UVB.Thus, the micro and nanoencapsulation solved some intrinsic problems related to sunscreens and rutin without, however, compromising their photohemoprotective ability, since the results showed similar or better efficacy when compared to the formulations containing actives in free form. Therefore, the development of formulations containing microencapsulated and nanoencapsulated compounds is an interesting alternative for employment in commercial products for sun protection by decreasing the undesirable characteristics, such as penetration and instability, improving the photoprotective properties and avoiding the need to develop new compounds with photoprotective characteristics.