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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
81

Hyperspectral Imaging for Nondestructive Measurement of Food Quality

Nanyam, Yasasvy 01 December 2010 (has links)
This thesis focuses on developing a nondestructive strategy for measuring the quality of food using hyperspectral imaging. The specific focus is to develop a classification methodology for detecting bruised/unbruised areas in hyperspectral images of fruits such as strawberries through the classification of pixels containing the edible portion of the fruit. A multiband segmentation algorithm is formulated to generate a mask for extracting the edible pixels from each band in a hypercube. A key feature of the segmentation algorithm is that it makes no prior assumptions for selecting the bands involved in the segmentation. Consequently, different bands may be selected for different hypercubes to accommodate the intra-hypercube variations. Gaussian univariate classifiers are implemented to classify the bruised-unbruised pixels in each band and it is shown that many band classifiers yield 100% classification accuracies. Furthermore, it is shown that the bands that contain the most useful discriminatory information for classifying bruised-unbruised pixels can be identified from the classification results. The strategy developed in this study will facilitate the design of fruit sorting systems using NIR cameras with selected bands.
82

CytoViva Hyperspectral Imaging for Comparing the Uptake and Transformation of AgNPs and Ag+ in Mitochondria

Steingass, Kristina 01 September 2021 (has links)
No description available.
83

Méthodes rapides de traitement d’images hyperspectrales. Application à la caractérisation en temps réel du matériau bois / Fast methods for hyperspectral images processing. Application to the real-time characterization of wood material

Nus, Ludivine 12 December 2019 (has links)
Cette thèse aborde le démélange en-ligne d’images hyperspectrales acquises par un imageur pushbroom, pour la caractérisation en temps réel du matériau bois. La première partie de cette thèse propose un modèle de mélange en-ligne fondé sur la factorisation en matrices non-négatives. À partir de ce modèle, trois algorithmes pour le démélange séquentiel en-ligne, fondés respectivement sur les règles de mise à jour multiplicatives, le gradient optimal de Nesterov et l’optimisation ADMM (Alternating Direction Method of Multipliers) sont développés. Ces algorithmes sont spécialement conçus pour réaliser le démélange en temps réel, au rythme d'acquisition de l'imageur pushbroom. Afin de régulariser le problème d’estimation (généralement mal posé), deux sortes de contraintes sur les endmembers sont utilisées : une contrainte de dispersion minimale ainsi qu’une contrainte de volume minimal. Une méthode pour l’estimation automatique du paramètre de régularisation est également proposée, en reformulant le problème de démélange hyperspectral en-ligne comme un problème d’optimisation bi-objectif. Dans la seconde partie de cette thèse, nous proposons une approche permettant de gérer la variation du nombre de sources, i.e. le rang de la décomposition, au cours du traitement. Les algorithmes en-ligne préalablement développés sont ainsi modifiés, en introduisant une étape d’apprentissage d’une bibliothèque hyperspectrale, ainsi que des pénalités de parcimonie permettant de sélectionner uniquement les sources actives. Enfin, la troisième partie de ces travaux consiste en l’application de nos approches à la détection et à la classification des singularités du matériau bois. / This PhD dissertation addresses the problem of on-line unmixing of hyperspectral images acquired by a pushbroom imaging system, for real-time characterization of wood. The first part of this work proposes an on-line mixing model based on non-negative matrix factorization. Based on this model, three algorithms for on-line sequential unmixing, using multiplicative update rules, the Nesterov optimal gradient and the ADMM optimization (Alternating Direction Method of Multipliers), respectively, are developed. These algorithms are specially designed to perform the unmixing in real time, at the pushbroom imager acquisition rate. In order to regularize the estimation problem (generally ill-posed), two types of constraints on the endmembers are used: a minimum dispersion constraint and a minimum volume constraint. A method for the unsupervised estimation of the regularization parameter is also proposed, by reformulating the on-line hyperspectral unmixing problem as a bi-objective optimization. In the second part of this manuscript, we propose an approach for handling the variation in the number of sources, i.e. the rank of the decomposition, during the processing. Thus, the previously developed on-line algorithms are modified, by introducing a hyperspectral library learning stage as well as sparse constraints allowing to select only the active sources. Finally, the third part of this work consists in the application of these approaches to the detection and the classification of the singularities of wood.
84

Určení obsahu rozpustných fenolických látek v porostech smrku ztepilého s využitím hyperspektrálních dat / Determination of soluble phenolics in common spruce stands using hyperspectral data

Buřičová, Michaela January 2011 (has links)
The thesis deals with lignin and soluble phenolic determination in Norway spruce foliace using hyperspectral data. A literature overview is focused on the analysis of lignin and soluble phenolics. The practical part focuses on the determination of wavelenghts intervals which are suitable for the detection of lignin and soluble phenolics. There is applied regression analysis for the determination of relationship between the foliage spectra and the content of biochemical substances for the chosen spektrum intervals. Indexes NDLI, mNDLI and RLI were than calculated. HyMap hyperspectral airborne images from 2009 and 2010 for the area of Sokolov, spectral curves of dry matter and fresh branches of Norway spruce and laboratory determination of lignin and soluble phenolics content were the inputs for the analyses. Maps of lignin content in Norway spruce are the final output of the work. Keywords: Norway spruce (Picea Abies), lignin, soluble phenolics, PLS (partial least square) method, multiple Stepwise regression, NDLI
85

Construction and development of a low-cost hyperspectral imaging system

Grigoriev, Nikita January 2022 (has links)
Quantification of spectral data is of great interest in many fields of science, since it can provide further insight into other properties of an object. However, traditional cameras are usually made to image the world in a similar fashion as to how we see it, wherefore they are usually not fit to record nor measure further spectral information. To get a better insight into the spectral properties of an object, a hyperspectral camera might be of use, since those can often identify and measure hundreds of different spectral bands. In this study we look at the construction and further development of an existing design of a push broom hyperspectral imaging system, built with optics for a fraction of the cost of commercial ones. With developed software and objects at hand a spectral calibration was performed, showing a possible spectral range of 184(2)-918(11) nm, but the use of the whole spectral range was however not possible due to limitations in the transmissivity of the lenses below 350 nm. A shift of the spectral range towards longer wavelengths is proposed, which would give further insight into the near infrared spectrum without any information losses. It was found that the spectral calibration of the imager was the main limiting factor of the system, since inaccuracies up to ±11 nm were identified, while the resolution has been found to be 1.4 nm in previous studies, proving that better calibrations are of essence. In good operating conditions, the resolution in the angle of view of the imager was found to be 0.55 mdeg. If the measurement conditions are not as good, or if such kind of spatial resolution is not required, a camera with a smaller detector size and larger pixels could be used to lower the cost of the system without a deterioration in image quality, since the uncertainties in the calibrations and measurement conditions were found to be the limiting factor.
86

Application of hyperspectral imaging combined with chemometrics for the non-destructive evaluation of the quality of fruit in postharvest

Munera Picazo, Sandra María 04 July 2021 (has links)
[ES] El objetivo de esta tesis doctoral es evaluar la técnica de imagen hiperespectral en el rango visible e infrarrojo cercano, en combinación con técnicas quimiométricas para la evaluación de la calidad de la fruta en poscosecha de manera eficaz y sostenible. Con este fin, se presentan diferentes estudios en los que se evalúa la calidad de algunas frutas que por su valor económico, estratégico o social, son de especial importancia en la Comunidad Valenciana como son el caqui 'Rojo Brillante', la granada 'Mollar de Elche', el níspero 'Algerie' o diferentes cultivares de nectarina. En primer lugar se llevó a cabo la monitorización de la calidad poscosecha de nectarinas 'Big Top' y 'Magique' usando imagen hiperespectral en reflectancia y transmitancia. Al mismo tiempo se evaluó la transmitancia para la detección de huesos abiertos. Se llevó a cabo también un estudio para distinguir los cultivares 'Big Top' y "Diamond Ray", los cuales poseen un aspecto muy similar pero sabor diferente. En cuanto al caqui 'Rojo Brillante', la imagen hiperespectral fue estudiada por una parte para monitorear su madurez, y por otra parte para evaluar la astringencia de esta fruta, que debe ser completamente eliminada antes de su comercialización. Las propiedades físico-químicas de la granada 'Mollar de Elche' fueron evaluadas usando imagen de color e hiperespectral durante su madurez usando la información de la fruta intacta y de los arilos. Finalmente, esta técnica se usó para caracterizar e identificar los defectos internos y externos del níspero 'Algerie'. En la predicción de los índices de calidad IQI y RPI usando imagen en reflectancia y transmitancia se obtuvieron valores de R2 alrededor de 0,90 y en la discriminación por firmeza, una precisión entorno al 95 % usando longitudes de onda seleccionadas. En cuanto a la detección de huesos abiertos, el uso de la imagen hiperespectral en transmitancia obtuvo un 93,5 % de clasificación correcta de frutas con hueso normal y 100 % con hueso abierto usando modelos PLS-DA y 7 longitudes de onda. Los resultados obtenidos en la clasificación de los cultivares 'Big Top' y 'Diamond Ray' mostraron una fiabilidad superior al 96,0 % mediante el uso de modelos PLS-DA y 14 longitudes de onda seleccionadas, superando a la imagen de color (56,9 %) y a un panel entrenado (54,5 %). Con respecto al caqui, los resultados obtenidos indicaron que es posible distinguir entre tres estados de madurez con una precisión del 96,0 % usando modelos QDA y se predijo su firmeza obteniendo un valor de R2 de 0,80 usando PLS-R. En cuanto a la astringencia, se llevaron a cabo dos estudios similares en los que en el primero se discriminó la fruta de acuerdo al tiempo de tratamiento con altas concentraciones de CO2 con una precisión entorno al 95,0 % usando QDA. En el segundo se discriminó la fruta de acuerdo a un valor de contenido en taninos (0,04 %) y se determinó qué área de la fruta era mejor para realizar esta discriminación. Así se obtuvo una precisión del 86,9 % usando la zona media y 23 longitudes de onda. Los resultados obtenidos para la granada indicaron que la imagen de color e hiperespectral poseen una precisión similar en la predicción de las propiedades fisicoquímicas usando PLS-R y la información de la fruta intacta. Sin embargo, cuando se usó la información de los arilos, la imagen hiperespectral fue más precisa. En cuanto a la discriminación del estado de madurez usando PLS-DA, la imagen hiperespectral ofreció mayor precisión, 95,0 %, usando la información de la fruta intacta y del 100 % usando la de los arilos. Finalmente, los resultados obtenidos para el níspero indicaron que la imagen hiperespectral junto con el método de clasificación XGBOOST pudo discriminar entre muestras con y sin defectos con una precisión del 97,5 % y entre muestras sin defectos o con defectos internos o externos con una precisión del 96,7 %. Además fue posible distinguir entre los dife / [CA] L'objectiu de la present tesi doctoral se centra en avaluar la capacitat de la imatge hiperespectral en el rang visible i infraroig pròxim, en combinació amb mètodes quimiomètrics, per a l'avaluació de la qualitat de la fruita en post collita de manera eficaç i sostenible. A aquest efecte, es presenten diferents estudis en els quals s'avalua la qualitat d'algunes fruites que pel seu valor econòmic, estratègic o social, són d'especial importància a la Comunitat Valenciana com són el caqui 'Rojo Brillante', la magrana 'Mollar de Elche', el nispro 'Algerie' o diferents cultivares de nectarina. En primer lloc es va dur a terme la monitorització de la qualitat post collita de nectarines 'Big Top' i 'Magique' per mitjà d'imatge hiperespectral en reflectància i trasnmitancia. Així mateix es va avaluar la transmitància per a la detecció d'ossos oberts. Es va dur a terme també un estudi per distingir els cultivares 'Big Top' i 'Diamond Ray', els quals posseeixen un aspecte molt semblant però sabor diferent. Pel que fa al caqui 'Rojo Brillante', la imatge hiperespectral va ser estudiada d'una banda per a monitoritzar la seua maduresa, i per un altre costat per avaluar l'astringència, que ha de ser completament eliminada abans de la seua comercialització. Les propietats fisicoquímiques de la magrana 'Mollar de Elche' van ser avaluades per la imatge de color i hiperespectral durant la seua maduresa usant la informació de la fruita intacta i els arils. Finalment, aquesta tècnica es va fer servir per caracteritzar i identificar els defectes interns i externs del nispro 'Algerie'. En la predicció dels índexs de qualitat IQI i RPI usant imatge en reflectància com en trasnmitancia es van obtindre valors de R2 al voltant de 0,90 i en la discriminació per fermesa una precisió entorn del 95,0 % utilitzant longituds d'ona seleccionades. Pel que fa a la detecció d'ossos oberts, l'ús de la imatge hiperespectral en transmitància va obtindre un 93,5 % classificació correcta de fruites amb os normal i 100 % amb os obert usant models PLS-DA i 7 longituds d'ona. Els resultats obtinguts en la classificació dels cultivares 'Big Top' i 'Diamond Ray' van mostrar una fiabilitat superior al 96,0 % per mitjà de l'ús de models PLS-DA i 14 longituds d'ona, superant a la imatge de color (56,9 %) i a un panell sensorial entrenat (54,5 %). Quant al caqui, els resultats obtinguts van indicar que és possible distingir entre tres estats de maduresa amb una precisió del 96,0 % usant models QDA i es va predir la seua fermesa obtenint un valor de R2 de 0,80 usant PLS-R. Pel que fa a l'astringència, es van dur a terme dos estudis similars en què el primer es va discriminar la fruita d'acord al temps de tractament amb altes concentracions de CO2 amb una precisió al voltant del 95,0 % usant QDA. En el segon, es va discriminar la fruita d'acord a un valor de contingut en tanins (0,04 %) i es va determinar quina part de la fruita era millor per a realitzar aquesta discriminació. Així es va obtindre una precisió del 86,9 % usant la zona mitjana i 23 longituds d'ona. Els resultats obtinguts per la magrana van indicar que la imatge de color i hiperespectral posseïxen una precisió semblant a la predicció de les propietats fisicoquímiques usant PLS-R i la informació de la fruita intacta. No obstant això, quan es va usar la informació dels arils, la imatge hiperespectral va ser més precisa. Quant a la discriminació de l'estat de maduresa usant PLS-DA, la imatge hiperespectral va oferir major precisió (95,0 %) usant la informació de la fruita intacta i del 100 % usant la dels arils. Finalment, els resultats obtinguts pel nispro indiquen que la imatge hiperespectral juntament amb el mètode de classificació XGBOOST va poder discriminar entre mostres amb i sense defectes amb una precisió del 97,5 % i entre mostres sense defectes o amb defectes interns o externs amb una precisió del 96,7 %. A més, va ser possible distingir entre / [EN] The objective of this doctoral thesis is to evaluate the potential of the hyperspectral imaging in the visible and near infrared range in combination with chemometrics for the assessment of the postharvest quality of fruit in a non-destructive, efficient and sustainable manner. To this end, different studies are presented in which the quality of some fruits is evaluated. Due to their economic, strategic or social value, the selected fruits are of special importance in the Valencian Community, such as Persimmon 'Rojo Brillante', the pomegranate 'Mollar de Elche', the loquat 'Algerie' or different nectarine cultivars. First, the quality monitoring of 'Big Top' and 'Magique' nectarines was carried out using reflectance and transmittance images. At the same time, transmittance was evaluated for the detection of split pit. In addition, a classification was performed to distinguish the 'Big Top' and 'Diamond Ray' cultivars, which look very similar but have different flavour. Whereas that for the 'Rojo Brillante' persimmon, the hyperspectral imaging was studied on the one hand to monitor its maturity, and on the other hand to evaluate the astringency of this fruit, which must be completely eliminated before its commercialization. The physicochemical properties of the 'Mollar de Elche' pomegranate were evaluated by means of hyperspectral and colour imaging during its maturity using the information from the intact fruit and arils. Finally, this technique was used to characterise and identify the internal and external defects of the 'Algerie' loquat. In the prediction of the IQI and RPI quality indexes using reflectance and transmittance images, R2 values around 0.90 were obtained and in the discrimination according to firmness, accuracy around 95.0 % using selected wavelengths was obtained. Regarding the split pit detection, the use of the hyperspectral image in transmittance mode obtained a 93.5 % of fruits with normal bone correctly classified and 100% with split pit using PLS-DA models and 7 wavelengths. The results obtained in the classification of 'Big Top' and 'Diamond Ray' fruits show accuracy higher than 96.0 % by using PLS-DA models and 14 selected wavelengths, higher than the obtained with colour image (56.9 %) and a trained panel (54.5 %). According to persimmon, the results obtained indicated that it is possible to distinguish between three states of maturity with an accuracy of 96.0 % using QDA models and its firmness was predicted obtaining a R2 value of 0.80 using PLS-R. Regarding astringency, two similar studies were carried out. In the first study, the fruit was classified according to the time of treatment with high concentrations of CO2 with a precision of around 95.0 % using QDA. In the second, the fruit was discriminated according to a threshold value of soluble tannins (0.04 %) and was determined what fruit area was better to perform this discrimination. Thus, an accuracy of 86.9 % was obtained using the middle area and 23 wavelengths. The results obtained for the pomegranate indicated that the use of colour and hyperspectral images have a similar precision in the prediction of physicochemical properties using PLS-R and the intact fruit information. However, when the information from the arils was used, the hyperspectral image was more accurate. Regarding the discrimination by the state of maturity using PLS-DA, the hyperspectral image offered greater precision, of 95.0 % using the information from the intact fruit and 100 % using that from the arils. Finally, the results obtained for the 'Algerie' loquat indicated that the hyperspectral image with the XGBOOST classification method could discriminate between sound samples and samples with defects with accuracy of 97.5 % and between sound samples or samples with internal or external defects with an accuracy of 96.7 %. It was also possible to distinguish between the different defects with an accuracy of 95.9 %. / Munera Picazo, SM. (2019). Application of hyperspectral imaging combined with chemometrics for the non-destructive evaluation of the quality of fruit in postharvest [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/125954 / TESIS
87

Monitoring crop development and health using UAV-based hyperspectral imagery and machine learning

Angel, Yoseline 07 1900 (has links)
Agriculture faces many challenges related to the increasing food demands of a growing global population and the sustainable use of resources in a changing environment. To address them, we need reliable information sources, like exploiting hyperspectral satellite, airborne, and ground-based remote sensing data to observe phenological traits through a crops growth cycle and gather information to precisely diagnose when, why, and where a crop is suffering negative impacts. By combining hyperspectral capabilities with unmanned aerial vehicles (UAVs), there is an increased capacity for providing time-critical monitoring and new insights into patterns of crop development. However, considerable effort is required to effectively utilize UAV-integrated hyperspectral systems in crop-modeling and crop-breeding tasks. Here, a UAV-based hyperspectral solution for mapping crop physiological parameters was explored within a machine learning framework. To do this, a range of complementary measurements were collected from a field-based phenotyping experiment, based on a diversity panel of wild tomato (Solanum pimpinellifolium) that were grown under fresh and saline conditions. From the UAV data, positionally accurate reflectance retrievals were produced using a computationally robust automated georectification and mosaicking methodology. The resulting multitemporal UAV data were then employed to retrieve leaf-chlorophyll (Chl) dynamics via a machine learning framework. Several approaches were evaluated to identify the best-performing regression supervised methods. An investigation of two learning strategies (i.e., sequential and retraining) and the value of using spectral bands and vegetation indices (VIs) as prediction features was also performed. Finally, the utility of UAVbased hyperspectral phenotyping was demonstrated by detecting the effects of salt-stress on the different tomato accessions by estimating the salt-induced senescence index from the retrieved Chl dynamics, facilitating the identification of salt-tolerant candidates for future investigations. This research illustrates the potential of UAV-based hyperspectral imaging for plant phenotyping and precision agriculture. In particular, a) developing systematic imaging calibration and pre-processing workflows; b) exploring machine learning-driven tools for retrieving plant phenological dynamics; c) establishing a plant stress detection approach from hyperspectral-derived metrics; and d) providing new insights into using computer vision, big-data analytics, and modeling strategies to deal effectively with the complexity of the UAV-based hyperspectral data in mapping plant physiological indicators.
88

Hyperspectral Remote Sensing for Winter Wheat Leaf Area Index Assessment in Precision Agriculture

Siegmann, Bastian 21 February 2017 (has links)
Remote sensing provides temporal, spectral and spatial information covering a wide area. Therefore, it has great potential in offering a detailed quantitative determination of the leaf area index (LAI) and other crop parameters in precision agriculture. The spatially differentiated assessment of LAI is of utmost importance for enabling an adapted field management, with the aim of increasing yields and reducing costs at the same time. The scientific focus of this work was the investigation of the potential of hyperspectral remote sensing data of different spectral resolutions, which were acquired at different spatial scales, for a precise assessment of wheat LAI. For this reason, three research experiments were conducted: 1) a comparison of different empirical-statistical regression techniques and their capabilities for a robust LAI prediction; 2) a determination of the required spectral resolution and important spectral regions/bands for precise LAI assessment; and 3) an investigation of the influence of the ground sampling distance of remote sensing images on the quality of spatial LAI predictions. The first part of this thesis compared three empirical-statistical regression techniques – namely, partial least-squares regression (PLSR), support vector regression (SVR) and random forest regression (RFR) – and their achieved model qualities for the assessment of wheat LAI from field reflectance measurements. In this context, the two different validation techniques – leave-one-out cross-validation (cv) and independent validation (iv) – were applied for verifying the accuracy of the different empirical-statistical regression models. The results clearly showed that model performance markedly depends on the validation technique used to assess model accuracy. In the case of leave-one-out cross-validation, SVR provided the best results, while PLSR proved to be superior to SVR and RFR when independent validation was applied. In the second part of this thesis, the spectral characteristics of the hyperspectral airborne sensor aisaDUAL (98 spectral bands) and the upcoming hyperspectral satellite mission EnMAP (204 spectral bands) were investigated to show their capability regarding the precise determination of wheat LAI. Moreover, the feature selection algorithm RReliefF, combined with a randomized sampling approach, was applied to identify the spectral bands that were most sensitive to changes in LAI. The results demonstrated that only three spectral bands of aisaDUAL, as well as EnMAP, at specific locations within the investigated spectral range (400–2,500 nm) were necessary for an accurate LAI prediction. The third part of this thesis dealt with the influence of the spatial resolution of aisaDUAL (3 m) and simulated EnMAP (30 m) image data on the assessment of wheat LAI. While the ground sampling distance (GSD) of aisaDUAL allowed a robust regression model calibration and validation, LAI predictions based on simulated EnMAP image data led to poor results because of the distinct difference in size between the EnMAP pixels (900 m2) and the sampled field plots (0.25 m2) for which the LAI was measured. In order to enable a more precise determination of wheat LAI from EnMAP image data, the two different approaches of image aggregation and image fusion were examined. In this context, the fusion approach has proven to be the more suitable method, which allowed a more accurate LAI prediction compared to the results based on the EnMAP data with a GSD of 30 m. In summary, the findings of the research reported in this thesis demonstrated that the accuracy of spatial LAI predictions from remote sensing data depends on several factors. Besides the applied empirical-statistical retrieval- and validation method, the spatial and spectral characteristics of the used image data sets played an important role. With the forthcoming hyperspectral satellite missions (e.g., EnMAP, HyspIRI), the area-wide assessment of LAI and other crop parameters (e.g., biomass, chlorophyll content) will be strongly supported. The moderate spatial resolutions of these satellites systems, however, require a combined use with spatial higher resolution multi- or superspectral satellite data (e.g., RapidEye, Sentinel-2). This multisensoral approach offers great potential for the prompt identification of spatial variations in crop conditions on sub-field scale, which is a mandatory prerequisite for precision agricultural applications.
89

In-field characterization of salt stress responses of chlorophylls a and b and carotenoid concentrations in leaves of Solanum pimpinellifolium

Ilies, Dragos-Bogdan 10 1900 (has links)
Food security is a major concern of the 21st century, given climate change and population growth. In addition, high salt concentrations in soils affect ~20% of irrigated land and cause a substantial reduction in crop yield. Cultivating salt-tolerant crops could enable the use of salt-affected agricultural land, reduce the use of fresh water and alleviate yield losses. Innovative methods need to be developed to study traditional and novel traits that contribute to salinity tolerance and accurately quantify them. These studies would eventually serve for developing new salt tolerant crops, adapted to the harsh arid and semi-arid climate conditions. A study of 200 accessions of the wild tomatoes (Solanum pimpinellifolium) was conducted in field conditions with phenotyping using an unmanned aerial vehicle (UAV)-mounted hyperspectral camera. Six genotypes with different levels of salt tolerance were sampled for leaf pigment analyses, revealing a clear pattern for the high salt tolerant accession M007, where pigment content in the salt-treated plants significantly increased compared to their control counterparts only in harvesting campaigns 3 and 6, each performed two days after the first and second salt stress application events. Moreover, the light harvesting capacity was found to be better maintained under salt stress in the medium (M255) and highly salt tolerant (M007 and M061) accessions. Pigment quantitation data will contribute towards the groundtruthing of hyperspectral imaging for the development of remote sensing-based predictive pigment mapping methods. This work establishes a reliable quantification protocol for correlating pigment content with vegetation indices. Hence, pigment content captured by imaging techniques and validated using biochemical analysis would serve in developing a high-throughput method for pigment quantitation in the field using UAV-based hyperspectral imaging. This would serve as a tool for measuring pigment content in large number of genotypes in the field which would eventually lead to new salt-tolerant genes.
90

Assessing and Enabling Independent Component Analysis As A Hyperspectral Unmixing Approach

Stites, Matthew R. 01 May 2012 (has links)
As a result of its capacity for material discrimination, hyperspectral imaging has been utilized for applications ranging from mining to agriculture to planetary exploration. One of the most common methods of exploiting hyperspectral images is spectral unmixing, which is used to discriminate and locate the various types of materials that are present in the scene. When this processing is done without the aid of a reference library of material spectra, the problem is called blind or unsupervised spectral unmixing. Independent component analysis (ICA) is a blind source separation approach that operates by finding outputs, called independent components, that are statistically independent. ICA has been applied to the unsupervised spectral unmixing problem, producing intriguing, if somewhat unsatisfying results. This dissatisfaction stems from the fact that independent components are subject to a scale ambiguity which must be resolved before they can be used effectively in the context of the spectral unmixing problem. In this dissertation, ICA is explored as a spectral unmixing approach. Various processing steps that are common in many ICA algorithms are examined to assess their impact on spectral unmixing results. Synthetically-generated but physically-realistic data are used to allow the assessment to be quantitative rather than qualitative only. Additionally, two algorithms, class-based abundance rescaling (CBAR) and extended class-based abundance rescaling (CBAR-X), are introduced to enable accurate rescaling of independent components. Experimental results demonstrate the improved rescaling accuracy provided by the CBAR and CBAR-X algorithms, as well as the general viability of ICA as a spectral unmixing approach.

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