Bakgrund: Hypotermi definieras som en kärntemperatur under 36 grader och är en vanlig komplikation i samband med anestesi. Hypotermi vid kirurgi är förknippat med ökad mortalitet och kan leda till komplikationer som innebär lidande, förlänger tid för återhämtning och orsakar ökade vårdrelaterade kostnader. Komplikationer på grund av hypotermi är exempelvis försämrad sårläkning, ökad risk för blödning och ökad incidens av infektioner. För många patienter innebär det ett lidande att frysa eftersom att vara kall är förknippat med känslor av utsatthet, sårbarhet och att få sämre vård. Olika metoder och hjälpmedel för att förhindra att hypotermi uppstår under anestesi finns att tillgå. I Sverige saknas specifika riktlinjer på nationell nivå. Forskning har visat att följsamheten till lokala riktlinjer är låg. Patienten behöver hjälp med att bibehålla adekvat kroppstemperatur och här har anestesisjuksköterskan en viktig uppgift. Syfte: Syftet var att sammanställa tillgängliga interventioner för att förhindra oavsiktlig intraoperativ hypotermi.  Metod: En strukturerad litteraturöversikt med narrativ analys genomfördes. Informationssökning gjordes i databaserna PubMed och CINAHL.  Resultat: Tre grupper av värmebevarande metoder identifierades: aktiv, passiv och invasiv uppvärmning. Den mest förekommande metoden var aktiv värmning med forced-air warming, som också verkade vara den mest effektiva metoden för att bibehålla normal kroppstemperatur och förhindra hypotermi. Skillnaderna i effektivitet mellan metoderna varierade. Ingen aktiv eller passiv metod förhindrade hypotermi helt. Slutsats: Trots att effektiva metoder för att förhindra oavsiktlig intraoperativ hypotermi finns och används är incidensen av hypotermi hög. Ytterligare forskning behövs för att undersöka orsaker till den höga incidensen. / Bakgrund: Hypotermi definieras som en kärntemperatur under 36 grader och är en vanlig komplikation i samband med anestesi. Hypotermi under operation är förknippad med ökad dödlighet och kan leda till komplikationer som skapar lidande, förlänger återhämtningstiden och orsakar ökade vårdrelaterade kostnader. Komplikationer på grund av hypotermi är till exempel försämrad sårläkning, ökad blödningsrisk och ökad förekomst av infektioner. För många patienter innebär frysning lidande eftersom förkylning är förknippad med känslor av exponering, sårbarhet och att få sämre vård. Olika metoder och hjälpmedel finns tillgängliga för att förhindra hypotermi uppstår under anestesi. I Sverige finns inga särskilda riktlinjer på nationell nivå. Forskning har visat att efterlevnaden av lokala riktlinjer är låg. Patienten behöver hjälp med att upprätthålla en tillräcklig kroppstemperatur, och här har anestesisjuksköterskan ett viktigt ansvar. Syfte: Målet var att sammanställa tillgängliga interventioner för att förhindra oavsiktlig intraoperativ hypotermi. Metod: En strukturerad litteraturstudie med narrativ analys genomfördes. En informationssökning gjordes i databaserna PubMed och CINAHL. Resultat: Tre grupper av värmebevarande metoder identifierades: aktiv, passiv och invasiv uppvärmning. Den vanligaste metoden var aktiv uppvärmning med påtvingad luftuppvärmning, vilket också visade sig vara den mest effektiva metoden för att upprätthålla normal kroppstemperatur och förhindra hypotermi. Skillnaderna i effektivitet mellan metoderna varierade dock. Ingen aktiv eller passiv metod förhindrade helt hypotermi. Slutsats: Även om effektiva metoder för att förhindra oavsiktlig intraoperativ hypotermi finns och används, är förekomsten av hypotermi hög. Ytterligare forskning behövs för att undersöka orsakerna till den höga förekomsten. Sökord: Anestesi, kroppstemperatur, värmebevarande metoder, förebyggande och kontroll av hypotermi, oavsiktlig hypotermi, intraoperativ vård, kvantitativ, litteraturöversikt, perioperativ vård, kirurgi.

Introdução: Por se tratar de uma falha médica com potencial implicação jurídica, a retenção inadvertida de corpos estranhos continua sendo subnotificada, o que dificulta o seu estudo e a sua compreensão. Como resultado, ainda se enfrenta um problema recorrente. Este estudo explorou a experiência de cirurgiões brasileiros em relação à retenção de corpos estranhos, analisando as suas características e consequências. Métodos: Foi enviado um questionário de preenchimento voluntário, confidencial e anônimo, por correio eletrônico, aos cirurgiões membros de nove sociedades brasileiras, durante um período de três meses. As questões analisaram a vivência dos entrevistados com os corpos estranhos, seus tipos, manifestações clínicas, diagnóstico, fatores de risco ou de proteção e implicações jurídicas. Resultados: Das 2872 submissões elegíveis, 43% dos médicos teriam deixado e 73% retirado corpos estranhos em uma ou mais ocasiões. Destes, 90% eram têxteis, 78% foram descobertos no primeiro ano e 14% eram assintomáticos. A maioria das retenções ocorreu no início da carreira profissional, em procedimentos eletivos (54%) e rotineiros (85%), porém complexos (57%). Emergência, ausência de contagem, pacientes obesos, fadiga do cirurgião e problemas relacionados às equipes cirúrgicas e aos processos foram tidos como os principais facilitadores. Os pacientes foram alertados sobre a retenção em 46% das vezes e, destes, 26% processaram os médicos ou a instituição. Conclusões: A maioria das retenções inadvertidas ocorreu nos primeiros anos de atividade profissional, em intervenções eletivas e rotineiras. Os corpos estranhos foram diagnosticados nos primeiros meses de pósoperatório, tendo sido os têxteis os mais frequentes. Os fatores de risco referidos pelos entrevistados são comuns em seus locais de trabalho, como emergências e equipes cirúrgicas incompletas, por exemplo. Menos de metade dos operados ficou ciente do evento adverso, sendo que a minoria acabou processando as instituições e/ou cirurgiões envolvidos / Background: Although there is an international mobilization to deal with unintentionally retained foreign bodies (RFB), since it is medical malpractice with potential legal implications, the cases are underreported, hindering the understanding and study of the problem. As a result, we face a recurrent and poorly understood event. This study explored the experience of brazilian surgeons on RFB and analyzed their characteristics and consequences. Study Design: In a three-month period, questionnaire was sent to surgeons members of nine brazilian societies, by electronic mail. Answering the questionnaire was volunteer. Answers were kept confidential and anonymous. The questions explored their experience with foreign bodies, FB types, clinical manifestations, diagnosis, risk and protection factors, and legal implications. Results: In 2872 eligible questionnaires, 43% of the doctors said they had already left FB and 73% had removed FB, in one or more occasions. Of these foreign bodies, 90% were textiles, 78% were discovered in the first year after the surgery and 14% remained asymptomatic. The occurrence of RFBs is more frequent in early professional career, in elective (54%) and routine (85%), but complex (57%) procedures. The main causes were emergency, lack of counting, inadequate work conditions, change of plans during the procedure and obese patients. Patients were alerted about the retention in 46% of the cases, and of these, 26% sued the doctors or the institution. Conclusion: The majority of unintentionally retained foreign bodies occurred at the beginning of the professional career, during routine surgical procedures. In general, foreign bodies caused symptoms and were diagnosed in the first year of the post-operative period. Textiles predominated. Inadequate work conditions were listed as RFB risk factors, as well as emergency surgery, for example. Less than half of the patients were aware of the adverse event and 26% sued the surgeons or the institutions involved in the procedure

Submitted by Emanuel Varela Cardoso (emanuel.varela@ufcg.edu.br) on 2018-06-29T20:52:05Z No. of bitstreams: 1 JOSÉ ALBERTO CAMPOS DA SILVA JÚNIOR – DISSERTAÇÃO (UAEMa) 2015.pdf: 3855504 bytes, checksum: 02bbfa20b694c9416d048edd8410bfd4 (MD5) / Made available in DSpace on 2018-06-29T20:52:05Z (GMT). No. of bitstreams: 1 JOSÉ ALBERTO CAMPOS DA SILVA JÚNIOR – DISSERTAÇÃO (UAEMa) 2015.pdf: 3855504 bytes, checksum: 02bbfa20b694c9416d048edd8410bfd4 (MD5) Previous issue date: 2015-03-17 / O monitoramento neurofisiológico intraoperatório (MNIO) é uma metodologia que agrega diferentes testes neurofisiológicos para uso simultâneo ou alternado num mesmo paciente durante o procedimento cirúrgico, podendo avaliar a neurofisiologia clínica em três campos: eletroencefalografia (EEG), eletromiografia (EMG) e potenciais evocados (PE). O potencial evocado visual (PEV) permite avaliar a função e integridade das estruturas corticais e subcorticais da via visual. Este exame é realizado cotidianamente nos laboratórios de neurofisiologia, auxiliando o esclarecimento de diferentes tipos de acometimentos da visão, seja por doença ou traumatismos. Este trabalho tem como objetivo desenvolver um protótipo para o monitoramento do PEV para avaliação da integridade das vias visuais durante cirurgias neurofisiológicas. Foram realizadas várias etapas para o desenvolvimento do protótipo do dispositivo ocular: simulação computacional; estudo das características do olho humano; prototipagem rápida; caracterização do Biopolímero ácido polilático (PLA) utilizado na prototipagem; desenvolvimento de um protótipo de dispositivo ocular com sistema de iluminação integrado por LEDs a realização dos testes de PEV com o protótipo desenvolvido. O PLA utilizado na prototipagem para o desenvolvimento de partes do dispositivo ocular foi caracterizado por Difração de Raios X (DRX), Espectroscopia na Região do Infravermelho por Transformada de Fourier (FTIR), Calorimetria Exploratória Diferencial (DSC), Microscopia Ótica (MO), Microscopia Eletrônica de Varredura (MEV) e Espectroscopia de Energia Dispersiva (EDS), esses resultados mostraram que a prototipagem rápida não alterou as propriedades físico-químicas e morfológicas do PLA. A simulação computacional forneceu parâmetros adequados ao desenvolvimento do dispositivo ocular que possibilitou uma maior eficiência na montagem do circuito eletrônico. Os resultados dos testes de PEV foram realizados em diferentes pacientes, com os olhos fechados e os mostraram-se promissores para uso em pacientes anestesiados. / Intraoperative neurophysiological monitoring (MNIO) is a methodology that combines different neurophysiological tests for simultaneous or alternating in the same patient during the surgical procedure can evaluate the clinical neurophysiology in three fields: electroencephalography (EEG), electromyography (EMG) and evoked potential (EP). The visual evoked potential (VEP) evaluates the function and integrity of cortical and subcortical structures of the visual pathway. This test is performed daily in neurophysiology laboratories, helping the clarifying of different types of bouts vision, whether by disease or trauma. This study aimed to develop a prototype for monitoring the VEP to assess the integrity of the visual pathways during neurophysiological surgeries. Several steps were performed for the prototype ocular device: computer simulation; study of the characteristics of the human eye; rapid prototyping; Biopolymer characterization of polylactic acid (PLA) used in prototyping; development of an ocular device prototype with integrated lighting system and VEP achievement tests with the prototype. The PLA used in the prototype for the development of parts of the device eye was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), optical microscopy (OM), Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS), these results showed that the rapid prototyping did not change the physical-chemical and morphological PLA. The computer simulation provides appropriate parameters for the development of ocular device that allowed greater efficiency in the assembly of electronic circuit. The results of ENP tests were performed in different patients, with closed eyes and have shown promise for use in anesthetized patients.

Introdução: Por se tratar de uma falha médica com potencial implicação jurídica, a retenção inadvertida de corpos estranhos continua sendo subnotificada, o que dificulta o seu estudo e a sua compreensão. Como resultado, ainda se enfrenta um problema recorrente. Este estudo explorou a experiência de cirurgiões brasileiros em relação à retenção de corpos estranhos, analisando as suas características e consequências. Métodos: Foi enviado um questionário de preenchimento voluntário, confidencial e anônimo, por correio eletrônico, aos cirurgiões membros de nove sociedades brasileiras, durante um período de três meses. As questões analisaram a vivência dos entrevistados com os corpos estranhos, seus tipos, manifestações clínicas, diagnóstico, fatores de risco ou de proteção e implicações jurídicas. Resultados: Das 2872 submissões elegíveis, 43% dos médicos teriam deixado e 73% retirado corpos estranhos em uma ou mais ocasiões. Destes, 90% eram têxteis, 78% foram descobertos no primeiro ano e 14% eram assintomáticos. A maioria das retenções ocorreu no início da carreira profissional, em procedimentos eletivos (54%) e rotineiros (85%), porém complexos (57%). Emergência, ausência de contagem, pacientes obesos, fadiga do cirurgião e problemas relacionados às equipes cirúrgicas e aos processos foram tidos como os principais facilitadores. Os pacientes foram alertados sobre a retenção em 46% das vezes e, destes, 26% processaram os médicos ou a instituição. Conclusões: A maioria das retenções inadvertidas ocorreu nos primeiros anos de atividade profissional, em intervenções eletivas e rotineiras. Os corpos estranhos foram diagnosticados nos primeiros meses de pósoperatório, tendo sido os têxteis os mais frequentes. Os fatores de risco referidos pelos entrevistados são comuns em seus locais de trabalho, como emergências e equipes cirúrgicas incompletas, por exemplo. Menos de metade dos operados ficou ciente do evento adverso, sendo que a minoria acabou processando as instituições e/ou cirurgiões envolvidos / Background: Although there is an international mobilization to deal with unintentionally retained foreign bodies (RFB), since it is medical malpractice with potential legal implications, the cases are underreported, hindering the understanding and study of the problem. As a result, we face a recurrent and poorly understood event. This study explored the experience of brazilian surgeons on RFB and analyzed their characteristics and consequences. Study Design: In a three-month period, questionnaire was sent to surgeons members of nine brazilian societies, by electronic mail. Answering the questionnaire was volunteer. Answers were kept confidential and anonymous. The questions explored their experience with foreign bodies, FB types, clinical manifestations, diagnosis, risk and protection factors, and legal implications. Results: In 2872 eligible questionnaires, 43% of the doctors said they had already left FB and 73% had removed FB, in one or more occasions. Of these foreign bodies, 90% were textiles, 78% were discovered in the first year after the surgery and 14% remained asymptomatic. The occurrence of RFBs is more frequent in early professional career, in elective (54%) and routine (85%), but complex (57%) procedures. The main causes were emergency, lack of counting, inadequate work conditions, change of plans during the procedure and obese patients. Patients were alerted about the retention in 46% of the cases, and of these, 26% sued the doctors or the institution. Conclusion: The majority of unintentionally retained foreign bodies occurred at the beginning of the professional career, during routine surgical procedures. In general, foreign bodies caused symptoms and were diagnosed in the first year of the post-operative period. Textiles predominated. Inadequate work conditions were listed as RFB risk factors, as well as emergency surgery, for example. Less than half of the patients were aware of the adverse event and 26% sued the surgeons or the institutions involved in the procedure

<div> Advancements in cancer treatments have increased rapidly in recent years, but cures remain elusive. Surgical tumor resection is a central treatment for many solid malignancies. Residual tumor at surgical margins leads to tumor recurrence. Novel tools for assessing residual tumor at surgical margins could improve surgical outcomes by helping to maximize the extent of resection. Ambient ionization-mass spectrometry (MS) methods generate and analyze ions from minimally prepared samples in near-real-time (e.g. seconds to minutes). These methods leverage the high sensitivity and specificity of mass spectrometry for analyzing gas phase ions and generating those ions quickly and with minimal sample preparation. Recent work has shown that differential profiles of ions, corresponding to phospholipids and small metabolites, are detected from cancerous and their respective normal tissue with ambient ionization-MS methods. When properly implemented, ambient ionization-MS could be used to assess for tumor at surgical margins and provide a molecular diagnosis during surgery. </div><div><br></div><div>The research herein reports efforts in developing rapid intraoperative ambient ionization-MS methods for the molecular assessment of cancerous tissues. Touch spray (TS) ionization and desorption electrospray ionization (DESI) were utilized to analyze kidney cancer and brain cancer.</div><div><br></div><div> As a demonstration of the applicability of TS-MS to provide diagnostic information from fresh surgical tissues, TS-MS was used to rapidly analyze renal cell carcinoma and healthy renal tissue biopsies obtained from human subjects undergoing nephrectomy surgery. Differential phospholipid profiles were identified using principal component analysis (PCA), and the significant ions were characterized using multiple stages of mass spectrometry and high resolution/exact mass MS. The same TS-MS analyzed renal tissues were subsequently analyzed with DESI-MS imaging to corroborate the TS-MS results, and the significant DESI-MS ions were also characterized with MS.</div><div><br></div><div>Significant efforts were made in developing and evaluating a standalone intraoperative DESI-MS system for analyzing brain tissue biopsies during brain tumor surgery. The intraoperative DESI-MS system consists of a linear trap quadrupole mass spectrometer placed on a custom-machined cart that contains all hardware for operating the mass spectrometer. This instrument was operated in the neurosurgical suites at Indiana University School of Medicine to rapidly analyze brain tissue biopsies obtained from glioma resection surgeries. A DESI-MS library of normal brain tissue and glioma was used to statistically classify the brain tissue biopsies collected in the operating room. Multivariate statistical methodologies were employed to predict the disease state and tumor cell percentage of the samples. A DESI-MS assay for detecting 2-hydroxyglutarate (2HG), the oncometabolic product of the isocitrate dehydrogenase (IDH) mutation (a key glioma prognostic marker), was developed and applied to determine the IDH mutation status during the surgical resection. The strengths, weaknesses, and areas of future work in this field are discussed. </div><div><br></div>

Epilepsy, as the most common chronic neurological disease, affects a significant part of population (0.5-1%). Drug resistant epilepsy has a significant negative effect on the quality of life, psychiatric comorbidities, neurocognitive performance and the risk of SUDEP in children. Therefore, resective epilepsy surgery, the only curative treatment of this condition, can fundamentally reverse this unfavorable prognosis. An inevitable prerequisite for a good postoperative result is complete removal of the epileptogenic zone (EC) and preservation of eloquent areas (EC). At present, even with improving and new preoperative non-invasive methods, we don't have an exclusive diagnostic method for theirs delineation. The aim of this PhD study is to assess benefit of individual intraoperative electrophysiological (iEF) methods in pediatric patients with focal intractable epilepsy. The first study evaluates the importance of intraoperative electrocorticography (iECoG) in the localization of EZ. The study proved that iECoG serves as a reliable tool to guide surgical resection and may predict results of epilepsy surgery. iECoG-based modification of surgical plan is not associated with increased risk of significant complications. The second presented study analyzed the contribution of intraoperative electrical...

<p>My research has focused on the development and translation of ambient ionization mass spectrometry (MS)-based platforms in clinical and surgical settings, specifically in the area of brain cancer diagnostics and surgical decision making. Ambient ionization MS methods, such as those described herein, generate and analyze gas phase ions with high sensitivity and specificity from minimally prepared samples in near-real-time, on the order of seconds to minutes, rendering them well suited to point-of-care applications. We used ambient ionization MS methods, specifically desorption electrospray ionization mass spectrometry (DESI-MS) and extraction nanoelectrospray ionization mass spectrometry (nESI-MS) to molecularly characterize brain cancer biopsies. The characterization was made using diagnostic compounds identified as markers of disease state, tissue composition, tumor type, and genotype in human brain tissue. Methods were developed and validated offline in the laboratory and translated to clinical and surgical settings, thereby generating chemical information on prognostic features intraoperatively and providing valuable information that would be otherwise unavailable. We believe that, with approval, the methodologies described can assist physicians and improve patient outcomes by providing analytical tools and molecular information that can inform surgical decision making and adjuvant treatment strategies, complementing and not interfering with standard of care protocols.</p> <p><br></p> <p>We have successfully demonstrated the use of desorption electrospray ionization mass spectrometry (DESI-MS) for the expedient molecular assessment of human glioma tissue biopsies based on lipid profiles and prognostic metabolites, both at the tumor core and near surgical margins, in two small-scale, clinical studies. Maximal surgical resection of gliomas that avoids non-infiltrated tissue is associated with survival benefit in patients with glioma. The infiltrative nature of gliomas, as well as their morphological and genetic diversity, renders treatment difficult and demands an integrated imaging and diagnostic approach during surgery to guide clinicians in achieving maximal tumor resection. Further, the estimation of tumor cell percentage (TCP), a measure of tumor infiltration at surgical margins, is not routinely assessed intraoperatively. </p> <p>We have previously shown that rapid, offline molecular assessment of tumor infiltration in tissue biopsies is possible and believe that the same assessment performed intraoperatively in biopsied tissue near surgical margins could improve resection and better inform patient management strategies, including postoperative radiotherapy. Using a DESI-MS spectral library of normal brain tissue and glioma biopsies to generate a statistical model to classify brain tissue biopsies intraoperatively, multivariate statistical approaches were used to predict the disease state and tumor cell percentage (TCP) of each biopsy, thereby providing an measure of tumor infiltration at surgical margins via molecular indicators. In addition to assessment of tumor infiltration, we have developed DESI-MS assays for detecting the oncometabolite 2-Hydroxyglutarate (2HG) to detect isocitrate dehydrogenase (IDH) mutations in gliomas intraoperatively. Knowledge of IDH genotypes at the time of surgical resection could improve patient outcomes, as more aggressive tumor resection of IDH-mutated gliomas is associated with increased survival. While assessments of IDH genotype are typically not available until days after surgery, we have demonstrated the ability to provide this information is less than five minutes. An intraoperative DESI-MS system has successfully been used in a proof-of-concept clinical study and intraoperative performance validation of this platform is ongoing. The findings of these two studies as well as strengths, weaknesses, and areas of improvement for upcoming future iterations of the research are discussed.</p> <p><br></p> <p>Point-of-care applications necessitate the adaptation of MS methodologies to smaller devices. Miniature mass spectrometers (Mini MS) boast small footprints, simple operation, and low power consumption, noise levels, and cost, making them attractive candidates for point-of-care use. In a small-scale clinical study, we demonstrated the first application of a Mini MS for determination of IDH mutation status in gliomas intraoperatively. This study paves a path forward for the application of Mini MS in the OR. With its small footprint and low power consumption and noise level, this application of miniature mass spectrometers represents a simple and cost-effective platform for an important intraoperative measurement. </p> <p><br></p> <p>While MS-based methods of tissue analysis can detect molecular features of interest and rapidly produce large quantities of data, their inherent speed is rarely utilized because they are traditionally coupled with time-consuming separation techniques (e.g., chromatography). Ambient ionization MS, specifically DESI-MS, is well suited for high-throughput applications due to its lack of sample preparation and purification techniques. In an attempt to rapidly characterize microarrays of tissue biopsies, we developed a high-throughput DESI-MS (HT-DESI-MS) method for the rapid characterization of disease state, human brain tumor type, glioma classification, and detection of IDH mutations in tissue microarrays (TMA) of banked and fresh human brain tissue biopsies. We anticipate that HT-DESI-MS analysis of TMAs could become a standard tool for the generation of spectral libraries for sample classification, the identification of biomarkers through large-scale studies, the correlation of molecular features with anatomical features when coupled to digital pathology, and the assessment of drug efficacy. </p>