My doctoral thesis challenges the traditional paradigm of phosphorus (P) limitation of phytoplankton communities in freshwaters by suggesting colimitation of P, nitrogen (N), and iron (Fe) in Great Lakes. Oceanographers have recognized Fe, N and P colimitation, and biomass response to Fe is documented in freshwater lakes. I studied African and North American Great Lakes that are similar to large inland oceans. I discovered that Fe is a key nutrient that is often limiting in the offshore, and may explain the dominance of cyanobacteria in nutrient enriched lakes. I also discovered that the nearshore and offshore areas of these large lakes are very different, particularly when invasive dreissenid mussels are impacting the nearshore, as seen in the eastern basin of Lake Erie. As a result of the dreissenids, chlorophyll a (chla) concentrations are significantly lower in the nearshore of Lake Erie, but higher in the nearshore in the three African Great Lakes, as well as pre-dreissenid Lake Erie. The objective of my thesis was to determine the limiting nutrient(s) to the phytoplankton of the Great Lakes Erie, Malawi, Tanganyika, and Victoria in both the nearshore and offshore by measuring the physiological status of the phytoplankton. I also examined how dreissenids affect the distribution of seston and nutrient concentrations between the nearshore and offshore of the eastern basin of Lake Erie. My study design included temporal and spatial surveys in the nearshore and offshore of the four lakes, in which I used a variety of nutrient limitation indicators for P (C:P, N:P, P debt, APA, Fv/Fm), N (C:N, NH4 debt, NO3 debt, Fv/Fm), and Fe (Fv/Fm), as well as photosynthetic efficiency (Fv/Fm) experiments. Nutrient enrichment experiments were also conducted in the nearshore and offshore of the eastern basin of Lake Erie which involved the addition and removal of Fe alone, as well as in combination with P and/or N. Lake Erie nutrient enrichment experiments provided evidence for P, N and Fe colimitation where the addition of Fe with P relieved Fe and P limitation and allowed nitrate (NO3-) assimilation, alleviating N limitation. However, the offshore experiments indicated stronger Fe limitation than the nearshore experiment. Lower chla concentrations in the post-dreissenid nearshore of the eastern basin of Lake Erie may not be due entirely to lower phytoplankton biomass, as photoacclimation of the phytoplankton may also be occurring. Dreissenid grazing effects can be seen in the distribution of dissolved nutrient concentrations between the nearshore and offshore of post-dreissenid Erie. The African Great Lakes are threatened by expanding human populations, resulting in increased nutrient runoff; the consequences of which will depend on the limiting nutrient(s). I found that the nearshore regions of Lakes Malawi and Tanganyika were colimited by N and P, while the offshore regions were colimited by N, P and Fe. The nearshore of Lake Victoria was colimited by light and N, while the offshore was colimited by N, P and Fe. Fe limitation only occurs in the offshore, and positive, significant relationships were found between total dissolved Fe concentrations and cyanobacteria. Continued P and Fe loading to the lakes will create a higher N demand that will result in a shift to N2-fixing cyanobacteria, which has serious consequences to human and ecosystem health as they are a poor nutritive food source and some are potentially toxigenic. The majority of studies conducted on Great Lakes involve offshore sampling, however, the less understood nearshore is where human impacts and activities are concentrated. I discovered there are significant differences between the nearshore and offshore, which has implications for water quality monitoring.

The relative contribution of allochthonous and autochthonous production in zooplankton nutrition has been of interest since the net heterotrophy of lakes was recognised to be common. I measured the 13C signature of epilimnetic CO2, particulate organic carbon (POC), and zooplankton in 27 north-temperate lakes in late summer and used the relationships between the POC and zooplankton 13C signatures and the CO2 signature to estimate the autochthonous contribution to these fractions of the plankton. My hypothesis was that POC and zooplankton signature would reflect the 13CO2 signature if they were autochthonous. Conversely, increasing allochthonous C would result in a 13C signature of POC or zooplankton that is increasingly influenced by the allochthonous 13C signature (δ-28‰) and decreasingly dependent on the CO2 signature. The average autochthonous contribution to epilimnetic POC was estimated to be between 62 and 75%. Epilimnetic zooplankton were, on average, between 77 and 91% autochthonous, indicating that zooplankton bias their feeding towards the autochthonous fraction of POC. On average, zooplankton were 1.2‰ enriched in 13C relative to POC, but their biased feeding on phytoplankton means that they can be depleted relative to POC in lakes where POC is highly depleted in 13C. The relationship between 13C POC and 13CO2 allowed us to estimate average photosynthetic fraction as -15.9‰. This estimate is independent of how much allochthonous C contributes to POC. Variation in photosynthetic fractionation was not a major contributor to differences among lakes in POC and zooplankton 13C signature. Allochthonous C is an important, although clearly secondary, source of C to zooplankton of these lakes in late summer. I expanded the above analysis by culling the literature for 13C stable isotope data of lake CO2, POC, and zooplankton. I found that, similar to the lakes that I had sampled, POC signature showed a strong influence of allochthonous C, and inferred that it was close to 50% allochthonous on average. I calculated an autochthonous fractionation of -14.1‰ for the metadata, which was similar to that of the lakes I sampled. While POC had a considerable allochthonous contribution, zooplankton signatures were strongly related to the CO2 signatures, suggesting that their carbon was mostly autochthonous. Therefore, while terrestrial inputs form a major portion of POC, zooplankton C, on average, was largely autochthonous. I also examined the differences in 13C/15N among zooplankton taxa, and differences in 13CO2, 13C/15N of POM, and 13C/15N of zooplankton with depth. There were small differences among the 15N of various taxa, and I did not detect differences in 13C amongst taxa. I found vertical heterogeneity was most marked in 13CO2 signatures, which generally depleted appreciably with increasing lake depth. The signatures of 13C POM and 13C zooplankton also generally depleted with depth, but much less so than did 13CO2. I interpret this as indicating that a large portion of POM and zooplankton C in the metalimnia and hypolimnia of these lakes is derived from C fixed in the epilimnia.

Radiocarbon datings in a previous study suggested that Moshyttan in Nora bergslag is the oldest blast furnace in Sweden and Europe. The aim of this study was specifically to study the origin of the Moshyttan blast furnace to answer the question: when was the blast furnace at Moshyttan established? To this end, a 2.5 m sediment record was collected from Fickeln, a lake 600 m downstream of Moshyttan, in March 2012. The geochemical properties of the sediment record were analyzed for major and trace elements using XRF. The organic content was calculated from the ash residue following the mercury analyses as a proxy for organic matter. Pollen and charcoal were analyzed using a standardized method. A age- depth model was created based on four radiocarbon datings of the sediment profile. The pollen data suggest that early land use consisted of forest grazing from about AD 220, and agriculture from about AD 880. An increase in Pb and charcoal particles about AD 880 indicates early metallurgy in the area. The first significant evidence of the establishment of a blastfurnace was between AD 1020 and AD 1090 marked by a decrease in organic content combined with a strong increase of ore related metals such as Pb, Zn, Cu and a strong increase of charcoal particles. Within the uncertainty of the age-depth modeling, the results from this study offers support to Wetterholms radiocarbon datings, thus making Moshyttan the oldest known blast furnace in Sweden and Europe.

This thesis develops, validates and applies an empirical model that provides the first spatially explicit estimates of gross and net phytoplankton production in Lake Victoria. Gross and net phytoplankton production are in turn used to estimate the maximum sustainable yield (MSY) of Lake Victoria's fishery following an empirical formula and the carbon efficiency transfer method. Chapter 2 presents results from three inshore areas where diurnal and sub-seasonal gross and net phytoplankton production was derived using an adapted version of the phytoplankton production model developed by Fee (1990). Spatial and temporal trends of chlorophyll (chl), PI parameters, the vertical attenuation of PAR (kPAR), Secchi depths (SD) and respiration rates are identified. kPAR and SD are highly correlated to chl within the euphotic zone, as well as to each other. Furthermore, the two PI parameters, PBM and aB, exhibit a strong linear relationship and both decline along an increasing chl gradient, presumably due to increased light-limitation, a taxonomic shift from diatoms to cyanobacteria with increasing chl as well as an increased need for biologically fixed nitrogen. These hypotheses are supported by observed synchronous changes in the PSII:PSI ratio of phytoplankton and changes in the chl-specific attenuation of PAR (kchl). Relationships are also derived between biomass-specific respiration rates (RB) with chl and PBM; similar to PI parameters RB decreases with increasing chl. Owing to these correlative trends, only one parameter is required to estimate gross phytoplankton production through the empirical model developed in this thesis. The empirical model predicts that gross phytoplankton production increases in a near linear fashion between chl of 0 to 10 mg. m-3, begins to flatten out as chl approaches 20 mg. m-3 and then slightly decreases when chl exceeds 40 mg. m-3 where the maximum PPG of 13. 1 g O₂. m-2. day-1 is reached and is in close agreement with a theoretical argument proposed by Talling (1965). Areal respiration and consequently net phytoplankton production are sensitive to chl within the mixed layer as well as mixed layer depths. Overall, the lakewide averages of gross and net phytoplankton production are 9. 68 and 2. 2 g O₂. m-2. day-1 respectively. Significant temporal variability was observed on sub-seasonal scales within the inshore of Lake Victoria, and changes in limnological parameters coincided with changes in water column temperatures in each of the three bays. In Fielding Bay, the availability of meteorological data revealed that strong nocturnal wind events decreased both the water column temperature and chl, while both parameters generally increased in the absence of any such wind event. Lateral exchange of water with deeper areas through strong wind events essentially flushes Fielding Bay causing the observed decreases in both the water column temperature and chl; this hydrodynamic event also influences other limnological parameters according to their respective correlative regression equations with chl. Spatial trends were also observed between inshore areas. The deepest area, Napoleon Gulf, has the lowest values of chl while the shallowest area, Inner Murchison Bay, has the highest chl as the mean depth of a bay sets an approximate upper limit on chl. With respect to diurnal variability, PI parameters decline through the day, kPAR increases over the day and no statistically valid trends were ascertained for chl and RB. Chapter three examined spatial and seasonal patterns of chlorophyll fluorescence, temperature, dissolved oxygen and water transparency from four lakewide cruises. Significant spatial variability of each parameter confirmed that lakewide data is required to generate spatially explicit estimates of phytoplankton production. Complex patterns in the thermal structure during each cruise illustrated that physical processes in Lake Victoria are at times more complex that a previously stated unidirectional hypothesis of warm water in the north and cool water in the south (Spigel and Coulter 1996), and these patterns influence spatial patterns in dissolved oxygen and Secchi depths. Similar to Chapter 2, estimates of chl within the mixed layer were highly correlated to mixed depths, while lakewide averages of chl are lower than previously reported offshore values (Mugidde 1993, 2001).

My doctoral thesis challenges the traditional paradigm of phosphorus (P) limitation of phytoplankton communities in freshwaters by suggesting colimitation of P, nitrogen (N), and iron (Fe) in Great Lakes. Oceanographers have recognized Fe, N and P colimitation, and biomass response to Fe is documented in freshwater lakes. I studied African and North American Great Lakes that are similar to large inland oceans. I discovered that Fe is a key nutrient that is often limiting in the offshore, and may explain the dominance of cyanobacteria in nutrient enriched lakes. I also discovered that the nearshore and offshore areas of these large lakes are very different, particularly when invasive dreissenid mussels are impacting the nearshore, as seen in the eastern basin of Lake Erie. As a result of the dreissenids, chlorophyll a (chla) concentrations are significantly lower in the nearshore of Lake Erie, but higher in the nearshore in the three African Great Lakes, as well as pre-dreissenid Lake Erie. The objective of my thesis was to determine the limiting nutrient(s) to the phytoplankton of the Great Lakes Erie, Malawi, Tanganyika, and Victoria in both the nearshore and offshore by measuring the physiological status of the phytoplankton. I also examined how dreissenids affect the distribution of seston and nutrient concentrations between the nearshore and offshore of the eastern basin of Lake Erie. My study design included temporal and spatial surveys in the nearshore and offshore of the four lakes, in which I used a variety of nutrient limitation indicators for P (C:P, N:P, P debt, APA, Fv/Fm), N (C:N, NH4 debt, NO3 debt, Fv/Fm), and Fe (Fv/Fm), as well as photosynthetic efficiency (Fv/Fm) experiments. Nutrient enrichment experiments were also conducted in the nearshore and offshore of the eastern basin of Lake Erie which involved the addition and removal of Fe alone, as well as in combination with P and/or N. Lake Erie nutrient enrichment experiments provided evidence for P, N and Fe colimitation where the addition of Fe with P relieved Fe and P limitation and allowed nitrate (NO3-) assimilation, alleviating N limitation. However, the offshore experiments indicated stronger Fe limitation than the nearshore experiment. Lower chla concentrations in the post-dreissenid nearshore of the eastern basin of Lake Erie may not be due entirely to lower phytoplankton biomass, as photoacclimation of the phytoplankton may also be occurring. Dreissenid grazing effects can be seen in the distribution of dissolved nutrient concentrations between the nearshore and offshore of post-dreissenid Erie. The African Great Lakes are threatened by expanding human populations, resulting in increased nutrient runoff; the consequences of which will depend on the limiting nutrient(s). I found that the nearshore regions of Lakes Malawi and Tanganyika were colimited by N and P, while the offshore regions were colimited by N, P and Fe. The nearshore of Lake Victoria was colimited by light and N, while the offshore was colimited by N, P and Fe. Fe limitation only occurs in the offshore, and positive, significant relationships were found between total dissolved Fe concentrations and cyanobacteria. Continued P and Fe loading to the lakes will create a higher N demand that will result in a shift to N2-fixing cyanobacteria, which has serious consequences to human and ecosystem health as they are a poor nutritive food source and some are potentially toxigenic. The majority of studies conducted on Great Lakes involve offshore sampling, however, the less understood nearshore is where human impacts and activities are concentrated. I discovered there are significant differences between the nearshore and offshore, which has implications for water quality monitoring.

The relative contribution of allochthonous and autochthonous production in zooplankton nutrition has been of interest since the net heterotrophy of lakes was recognised to be common. I measured the 13C signature of epilimnetic CO2, particulate organic carbon (POC), and zooplankton in 27 north-temperate lakes in late summer and used the relationships between the POC and zooplankton 13C signatures and the CO2 signature to estimate the autochthonous contribution to these fractions of the plankton. My hypothesis was that POC and zooplankton signature would reflect the 13CO2 signature if they were autochthonous. Conversely, increasing allochthonous C would result in a 13C signature of POC or zooplankton that is increasingly influenced by the allochthonous 13C signature (δ-28‰) and decreasingly dependent on the CO2 signature. The average autochthonous contribution to epilimnetic POC was estimated to be between 62 and 75%. Epilimnetic zooplankton were, on average, between 77 and 91% autochthonous, indicating that zooplankton bias their feeding towards the autochthonous fraction of POC. On average, zooplankton were 1.2‰ enriched in 13C relative to POC, but their biased feeding on phytoplankton means that they can be depleted relative to POC in lakes where POC is highly depleted in 13C. The relationship between 13C POC and 13CO2 allowed us to estimate average photosynthetic fraction as -15.9‰. This estimate is independent of how much allochthonous C contributes to POC. Variation in photosynthetic fractionation was not a major contributor to differences among lakes in POC and zooplankton 13C signature. Allochthonous C is an important, although clearly secondary, source of C to zooplankton of these lakes in late summer. I expanded the above analysis by culling the literature for 13C stable isotope data of lake CO2, POC, and zooplankton. I found that, similar to the lakes that I had sampled, POC signature showed a strong influence of allochthonous C, and inferred that it was close to 50% allochthonous on average. I calculated an autochthonous fractionation of -14.1‰ for the metadata, which was similar to that of the lakes I sampled. While POC had a considerable allochthonous contribution, zooplankton signatures were strongly related to the CO2 signatures, suggesting that their carbon was mostly autochthonous. Therefore, while terrestrial inputs form a major portion of POC, zooplankton C, on average, was largely autochthonous. I also examined the differences in 13C/15N among zooplankton taxa, and differences in 13CO2, 13C/15N of POM, and 13C/15N of zooplankton with depth. There were small differences among the 15N of various taxa, and I did not detect differences in 13C amongst taxa. I found vertical heterogeneity was most marked in 13CO2 signatures, which generally depleted appreciably with increasing lake depth. The signatures of 13C POM and 13C zooplankton also generally depleted with depth, but much less so than did 13CO2. I interpret this as indicating that a large portion of POM and zooplankton C in the metalimnia and hypolimnia of these lakes is derived from C fixed in the epilimnia.

The main objective of this study was to investigate small-bodied fish caging as an approach to evaluate selenium (Se) bioaccumulation and speciation in native fish species inhabiting lakes influenced by uranium (U) milling effluent in northern Saskatchewan, Canada. In contaminated environments freshwater fish show a high propensity to accumulate Se beyond levels needed for normal physiological function. Maternal transfer of elevated Se concentrations to offspring can cause deformities and reduced survival in fry, and in certain cases negatively impact the sustainability of native fish populations. This research included a caging validation study using wild, naïve (i.e., collected from a reference lake) lake chub (Couesius plumbeus) and spottail shiner (Notropis hudsonius), and three field based 21-day caging studies to investigate the dominance of the feeding pathway with respect to Se uptake and speciation in wild populations of northern small-bodied fish exposed to a gradient of Se. Three feeding regimes were used: an in situ benthic diet, a basal Se diet of Chironomus dilutus (1.5 µg Se/g dry weight) and a Se-spiked diet of C. dilutus (5.5 µg Se/g dry weight). Lake chub were identified as more suitable candidates for caging due to higher survival and condition factor at the completion of the in situ 21-day trial. The resulting Se bioaccumulation was compared among treatments as well as to wild small-bodied fish populations from the study area. Results from the caging experiments showed that caged lake chub exposed to natural and controlled diets with elevated Se had significantly greater whole-body Se concentrations after 21 days compared to fish caged in the reference lake. The results also showed that whole-body Se concentrations exceeded conservative Se thresholds, and approached the currently proposed USEPA regulatory threshold (7.91 µg/g dry weight) designed to protect fish species in only three weeks. The use of stable carbon (C), nitrogen (N), and sulphur (S) isotope ratios indicated that alternate benthic food sources native to the exposure lake were consumed in conjunction with the controlled diets. Stable isotope analysis of both wild and caged lake chub indicated that the N and S isotopic signatures decreased with increasing Se exposure, representing differences in isotopic signatures of the food sources. Speciation results from caged lake chub indicated that Se substituted for S in methionine (i.e. selenomethionine) was the dominant Se species found in caged lake chub exposed to dietary sources of elevated Se. Overall, this research demonstrates that using caged native lake chub represents a useful biomonitoring approach to investigate patterns of Se bioaccumulation and speciation in fish.

Poyang Lake is continuously shrinking and losing its function on account of deforestation and reclamation, causing water quality deterioration, frequent floods and ecological damages that impede economic growth. The research mainly analyzes the cause of Poyang Lake reclamation, its development, and pros and cons through historical literatures. The fact is found from 13th -19th centuries that farmers individually reclaimed the lake had caused external uneconomic problems. It not only negatively impacted local ecology but resulted in economic burden to the local government, who originally considered that the reclamation could bring in tax increases; however, the local financial resources are not sufficient to resolve existing problems. Under the contradictions among economic development, ecological protection, individual benefits and the overall security, the problems will continuously exist. The results of this research give an overall introduction concerning Poyang Lake in China. By illustrating the causes and impacts of reclamation and governances for lake, a relation among a specific political system, economic growth, ecological changes and policies can be demonstrated and underlying factors of flood disaster in China can be summarized.

The goal of the Lake Ontario Maritime Cultural Landscape project was to investigate the nature and distribution of archaeological sites along the northeast shoreline of Lake Ontario while examining the environmental, political, and cultural factors that influenced the position of these sites. The primary method of investigation was a combined archaeological and historical survey of the shoreline within seven 1-km square areas. The archaeological component of the survey covered both the terrestrial and submerged portions of the shore through marine remote sensing (side-scan sonar and magnetometer), diving surveys, pedestrian surveys, and informant interviews. A total of 39 sites and 51 isolated finds were identified or further analyzed as a result of this project. These sites ranged from the Middle Archaic period (ca. 5500-2500 B.C.) through the 19th century and included habitation, military, transportation, and recreational sites. Analysis of these findings was conducted at two scales: the individual survey area and Lake Ontario as a whole. By treating each survey area as a distinct landscape, it was possible to discuss how various cultures and groups used each space and to identify instances of both dynamism and continuity in the landscapes. Results of these analyses included the continuous occupation of several locations from pre-Contact times to the present, varying uses of the same environment in response to political and economic shifts, the formation of communities around transportation nodes, and recurring settlement patterns. The survey data was also combined to explore regional-scale trends that manifest themselves in the historical Lake Ontario littoral landscape including ephemeral landscapes, permeable boundaries, danger in the lake, and factors of change.

Nuisance aquatic vegetation (mainly Hydrilla Verticillata ) has become problematic in Lake Conroe, TX. Consequently, triploid grass carp (Ctenopharynogodon idella) were stocked at densities sufficient to completely denude the reservoir of all vegetation (invasive and native plants) within one year. As a result, an assessment was designed to investigate the changes (before and after carp stocking) in the plant assemblage among sampling stations, changes in water quality parameters, length frequency and condition changes of Centrachid species, largemouth diet changes, and changes in the fish assemblages among randomly selected sampling stations between early fall 2007, when grass carp were stocked, and one year later in early fall of 2008. The areas for sampling were based upon aquatic vegetation surveys by Texas Parks and Wildlife during 2007 and 2008, thirteen sampling stations were randomly selected using ArcGIS software and the percentage of water surface covered by vegetation was recorded at each station. Within each station, fish were collected by electrofishing the entire station for five minutes; water samples were also collected. Largemouth bass diet did significantly change for mature (<200 mm-TL) bass as indicated by a chi-square test. Largemouth bass from the samples were shown to consume less sunfish and more shad by the second (post-carp) sample. This is consistent with expected results due to the removal of vegetation consequently eliminating small sunfish habitat. In similar fashion, significant length-frequency changes were seen in the second year as there were fewer smaller (juvenile) Centrachid species found in the sampling sites. Contrary to the Centrachids, length-frequency of gizzard shad significantly decreased in size by the second sampling year. Based upon the aquatic vegetation surveys within the sampling sites of 2007 and 2008, there was an almost complete elimination of all aquatic plants following carp introduction. This result was consistent with what was expected from the carp introductions. Changes in water quality parameters (phosphorous, nitrate, nitrites, orthophosphate, chlorophyll (a)), were generally inconclusive, with the exception of nitrate which significantly increased by the second year. The water quality parameters along with other measured habitat parameters were used in the multivariate analysis.