A urina é importante na comunicação olfativa de mamíferos, especialmente em roedores: apresenta baixo custo e contém metabólitos potencialmente informativos. Possibilita que o receptor avalie características do emissor (p. ex. espécie, sexo, idade, status de dominância e de saúde, receptividade sexual e o tempo da emissão) a partir da urina encontrada. O balanço hídrico, no entanto, pode ser desvantajoso com o uso da urina na marcação por animais de ambientes xéricos e quentes, já que existe um tradeoff entre o volume de água acessível/ingerido e o emitido na micção/marcação, com pressão seletiva a favor da economia hídrica. Nestes casos pode ocorrer redução na emissão de volume com consequente limitação do uso da marcação por urina. Trinomys yonenagae (Rocha, 1995) (Rodentia, Echimyidae) é altamente afiliativa, endêmica da Caatinga, e pertencente a um gênero de ancestral silvícola, cuja maioria das espécies apresenta fidelidade a ambientes florestados. Produz urina de média osmolaridade e de volume semelhante ao de espécies silvícolas do gênero, embora viva em um paleodeserto de temperatura elevada. Nesse contexto hipotetizamos que T. yonenagae faz uso de marcação por urina, uma estratégia importante para roedores sociais, explorando favoravelmente a restrição filogenética (produção de urina de média osmolaridade) imposta por sua ancestralidade. O padrão de deposição de urina e de fezes de T. yonenagae (n=10) e da espécie-irmã, Trinomys setosus (n=10), de ambiente florestado, foi visualizado sob luz ultravioleta (UV), em sessões individuais (24h), em gaiolas-padrão (40x33x16cm), forradas com papel de filtro. O mesmo procedimento foi usado nas investigações posteriores - a quantificação do número de fezes e das marcas de urinas, com estimativas do volume de marcação (VUt=L total/sessão e L/marca) - em T. yonenagae pré-pubertal (32 a 76d, n=10) e em adultos (um a 11 anos, n=21), de ambos os sexos, em sessões (10min) individuais e em contexto social. Nesse caso os rabos-de-facho adultos foram pareados em compartimentos adjacentes (caixa de 40x30x26cm), com um coespecífico de mesmo sexo, conhecido e desconhecido, e de sexo oposto, desconhecido. Qualitativamente as espécies-irmãs apresentam padrão de disposição de urina semelhantes, provavelmente herdados do ancestral silvícola comum. Há depósitos de dois tipos: marcas grandes, nos cantos da gaiola, e marcas pequenas, distribuídas na área central. Em T. yonenagae, as marcas grandes (VUt=3,00-750,00L, 6,67±4,30% do volume urinário diário), correlacionam-se significativamente (Pearson=0,41; sig.<0,00) com a deposição de fezes, sugerindo deposição de latrina; o VUt das marcas pequenas é de 0,01 a 0,05L. O número de marcas pequenas mais elevado, entre os sujeitos que urinaram, foi de quando pareadas com conhecidas (ANOVA um fator, F=3,70 sig.<0,00), sugerindo coesão social em , e associado a um pequeno investimento hídrico. Observou-se também depósitos de secreções da Glândula Harderiana (GH), que não se correlacionaram com nenhuma das variáveis analisadas. Não ocorreram diferenças estatisticamente significantes nas demais comparações feitas. Em condição solitária, 80% dos indivíduos adultos marcaram (latência <5min), enquanto que o grupo de 26-41d apenas dois sujeitos marcaram. O VUt correlacionouse com a idade positivamente (Pearson=0,64; sig.=0,05), entre os indivíduos jovens, e negativamente entre os adultos (Pearson=-0,39; sig.<0,00), o que sugere que a marcação por urina possa ser hormônio-dependente, como ocorre em outras espécies de roedores. A limitação ao acesso à água no ambiente natural dos rabos-de-facho parece não ter se sobreposto aos fatores de ancestralidade e comunicação social na modulação do comportamento de marcação por urina em T. yonenagae, especialmente para fêmeas que ocupam papel diferenciado na hierarquia social do grupo. O trabalho também salienta a ausência de hierarquia linear nos rabos-de-facho, como anteriormente relatado, e evidencia a presença de atividade da GH, abrindo nova linha de estudos fisiológicos e comportamentais dessa glândula em ratos-de-espinho / Urine is important in olfactive communication in mammals, especially in rodents: its presents low costs and had potentially informatives metabolites. The urine enables the sender to transmit informations about itself (p. ex. species, sex, age, dominance and health status, sexual receptivity, and time of deposit). The water balance, however, can be disadvantageous with the use of urine marking in xeric and hot environments, because there is a trade-off between the amount of water available/ingested and delivered in urination/marking, with pressure selective in favor of the water economy. In these cases there may be a reduction in emission of the volume with consequent limitation of the use of the urine in marking. Trinomys yonenagae (Rocha, 1995) (Rodentia, Echimyidae) is highly affiliative, endemic in the Caatinga, and belongs to a genus of forestry ancestral, which most of species have congruence with forested environments. The rabo-de-facho produces urine with medium osmolarity and volume similar to that of forestry species in the genus, although lives in a high temperature paleodesert. In this context we hypothesized that T. yonenagae marks with urine, an important strategy for social rodents, and explore favorably the phylogenetic constraint (production of medium osmolarity urine) imposed by their ancestral. The pattern of urine and feces deposition of T. yonenagae (n=10) and of the sister-species, Trinomys setosus (n=10), of forested environment, was visualized under ultraviolet light (UV) in individual sessions (24h) in house-cages (40x33x16cm), with the floor covered with filter paper. The same procedure was used in further investigations quantification of the number of feces and of the urine marks, with estimation of the urine volume (VUt = L total/session and L/mark) in pre-puebertal (32 to 76d, n = 10) and adults (one to 11 years, n = 21) T. yonenagae, of both sexes, in 10min sessions under individual and social context. The adults in social context was pared, in an adjacent compartments (of 40x30x26cm box), with a same-sex conspecific, known and unknown, and opposite, sex unknown. Qualitatively, both species shows similar patterns of urine deposition, probably inherited from the forestry ancestral. There are kinds of deposits: larger ones, deposited in the corners of the cage, and small ones, distributed in the central area. In T. yonenagae, the larger marks (VUt = 3,00-750,00L, 6.67 ± 4.30% of the daily urine volume) are significantly correlated (Pearson = 0.41, sig <0.00. ) with the number of feces pellets, suggesting latrine deposition; the VUt of small marks is from 0.01 to 0,05L. The higher number of small marks among subjects who urinated was in paired with known situation (one-way ANOVA, F = 3.70; sig. <0.00), suggesting social cohesion in , and associated with a small water investment. It is also noted deposits of Harderian gland (GH) secretions, which were not correlated with any of the variables. There were no statistically significant differences in the other comparisons. In solitary condition, 80% of adults marked (latency <5min), while only two subjects in the 26-41d group marked. The VUt correlated positively with age (Pearson = 0.64;. sig = 0.05) among young individuals, and negatively among adults (Pearson = -0.39;. sig <0.00). This suggests urine-marking in T. yonenagae as a hormone-dependent behavior, like in other rodent species. The limited access to water in the natural environment of rabo-de-facho seems to have not overlapped the ancestry factors and social communication in the modulation of urine for marking behavior, especially for females occupying distinct role in the social hierarchy the group. The work also highlights the absence of linear hierarchy in rabo-de-facho, as previously reported, and shows the presence of GH activity, opening new line of physiological and behavioral studies of this gland in spiny-rats

京都大学 / 新制・課程博士 / 博士(地球環境学) / 甲第24986号 / 地環博第247号 / 新制||地環||49(附属図書館) / 京都大学大学院地球環境学舎環境マネジメント専攻 / (主査)教授 越後 信哉, 准教授 田中 周平, 教授 藤原 拓, 准教授 原田 英典 / 学位規則第4条第1項該当 / Doctor of Global Environmental Studies / Kyoto University / DFAM

Access to clean water and improved sanitation are basic human right. This quantitative, descriptive study sought to establish current water and sanitation coverage in Chirumanzu and Shurugwi districts in Zimbabwe and develop methods of assessing coverage using Geographic Information Systems. Google Earth was used to identify homesteads. Personal digital assistant-based forms were used to collect geo-referenced data on all water points and selected households. Geospatial analysis methods were used to calculate borehole water coverage. Using Google Earth, 29375 homesteads were identified. The water survey mapped 4134 water points; 821 were boreholes; and only 548 were functional. Functional borehole water coverage was: 57.3%, 46.2%, and 33.5% for distance from household to water point of within 1500 m, 1000 m, and 500 m respectively. Sanitation coverage was 44.3%, but 96% of the latrines did not meet Blair Ventilated Pit latrine standards. / Health Studies / M.A. (Public Health) (Medical Informatics)

Effective management of the excreta or faecal sludge (FS) emptying, transport and disposal mechanisms from the on-plot latrines in urban areas of many low-income countries is critical for the sustainability of urban sanitation. However the literature mentions the lack of an effective management system for urban FS emptying, transport and disposal in the low-income countries. The current management of FS has been fragmented and improper with attendant poor health and environmental pollution problems. In particular, no substantive information was found relating to how far the excreta or sludge removed from the latrines is transported to the disposal points. Also information on household financial needs and their perceptions regarding emptying and transport services was lacking. No study had been done regarding the effects of disposal distance and accessibility on the cost of emptying and transport; neither has work been done on FS reuse implications for emptying, transport and disposal mechanisms. Based on these issues, the research questions and hypothesis were formulated to guide the study. Qualitative and quantitative research techniques were used to triangulate and ensure the reliability and validity of the findings and analysis. From the analysis of the findings, the thesis concludes on these key issues: i) Emptying, transport and disposal mechanisms of the excreta and FS as well as the costs of these depend on the type of latrines, latrine use and the technology available for emptying and transport. ii) Owing to the nature of latrines and the emptying methods used the disposal of the FS was indiscriminate and much was found closer to its source of generation. iii) FS was in high demand for reuse but lacked appropriate marketing strategy that could match supply with the demand. iv) The Household Centred Environmental Sanitation (HCES) approach was found to be limited in content and capacity to effectively address the urban excreta and FS emptying, transport and disposal without the active and full involvement of the municipal and local authorities with clear roles and regulations that address the key processes, linkages, and capacity development issues. Thus, the HCES approach needs periodic review and modifications to take care of the new developments and peculiarities of each urban setting. The study also recommends the need to look at streamlining technologies and developing capacity to address cross-cutting issues in urban sanitation. It further recommends the need for households, the sanitation authorities and practitioners to understand the links between latrine technology in terms of type, size, use and location vis-à-vis the required emptying, transport and disposal mechanisms in the urban areas of the low-income countries.

Engineers are exploring a new paradigm in wastewater treatment; focus is shifting to the recovery and reuse of energy, water, and nutrients. Ecological sanitation (EcoSan) technologies, which allow for this recovery and reuse, are an environmentally sound option for the future of sanitation. While the technology to achieve this goal of recovery and reuse exists, a limiting factor is user attitudes and perceptions. Social sciences, especially anthropology, can and should inform engineering projects to ensure socio-cultural sustainability. Since 2003, rural indigenous Ngäbe communities in Panama have been implementing ecological sanitation projects, mainly double vault urine diverting (DVUD) latrines known as composting latrines. With the help of governmental agencies and the Peace Corps, over 200 of these latrines have been built across the province of Bocas del Toro and the ñÖ Kribu region of the Comarca Ngäbe-Buglé. To this point, little monitoring and evaluation has taken place in these communities. Interviews and observations in 23 communities throughout this coastal region revealed that 70.6% of composting latrines constructed (n = 201) were completed and 71.8 % of the completed composting latrines (n = 142) are still in use. Based on observations, 65% of the latrines in use were determined to be used properly, which translates to the proper use of 45.8% of the completed latrines. To promote composting latrine adoption, social marketing and pilot latrine projects can be employed, and to improve the percentage of properly used composting latrines, education campaigns can be deployed as follow up. Utilizing suggestions made in recent literature as guidelines for the proper application of compost, analysis showed that new training messages have not reached the communities with older composting latrines. Informal interviews in 18 communities identified compost production, the lack of mosquitoes and flies, and the lack of odor as the most frequently mentioned advantages. With respect to the disadvantages, the inability to use water for anal cleansing was the most frequently mentioned disadvantage. In three communities, informal interviews and 124 surveys were used to characterize the perceptions of Ngäbes regarding feces and their use of composted human excrement as a soil amendment in agriculture. In general, the responses reflected perceptions that show no strong barrier to the operation and maintenance of composting latrines. Utilizing the Fisher's exact test and Kruskal-Wallis test, the community, sanitation classification, gender, primary occupation, and age all showed some level of association with the perceptions expressed in the survey responses. Filo Verde was more likely to respond with perceptions accepting of composting latrine use, while San San Puente was more likely to respond with "don't know" or with perceptions objecting to composting latrine use. At times, up to 37.9% of the respondents responded with negative perceptions; thus, evaluations of perceptions prior to the implementation stage are still beneficial. One discrepancy existed between the overall majority and the composting latrine user majority; 56.5% of the 124 respondents perceived the handling of human excrement as a great health risk, whereas 59.1% of the 22 composting latrine users did not. As expected, the composting latrine users responses represent the positive perceptions of feces and their reuse, but pit latrine owners were most likely to respond with perceptions contrary to those indicative of proper composting latrine behavior. Overall, males were more likely to agree with the perceptions related to composting latrine use. Regarding primary occupations, farmers consistently replied with more favorable perceptions of feces and their use as a soil amendment, while banana company workers showed more dissidence. Additionally, older participants gave responses reflecting favorable perceptions of composting latrines more than younger participants. Finally, education and household size do not have any statistically significant associations with the perceptions reflected in the survey responses.

Access to clean water and improved sanitation are basic human right. This quantitative, descriptive study sought to establish current water and sanitation coverage in Chirumanzu and Shurugwi districts in Zimbabwe and develop methods of assessing coverage using Geographic Information Systems. Google Earth was used to identify homesteads. Personal digital assistant-based forms were used to collect geo-referenced data on all water points and selected households. Geospatial analysis methods were used to calculate borehole water coverage. Using Google Earth, 29375 homesteads were identified. The water survey mapped 4134 water points; 821 were boreholes; and only 548 were functional. Functional borehole water coverage was: 57.3%, 46.2%, and 33.5% for distance from household to water point of within 1500 m, 1000 m, and 500 m respectively. Sanitation coverage was 44.3%, but 96% of the latrines did not meet Blair Ventilated Pit latrine standards. / Health Studies / M.A. (Public Health) (Medical Informatics)

Tierknochenfunde mit jeweils etwa 350 bestimmbaren Fragmenten aus vier mittelalterlichen und frühneuzeitlichen Gruben- und Grabenanlagen Güstrows (Mecklenburg-Vorpommern) werden vorgestellt. Sie weisen sämtlich einen hohen Anteil an Schlacht- und Speiseresten auf, gleichzeitig finden sich in ihnen aber auch Teilskelette deponierter oder hineingefallender Tiere, nicht zuletzt zahlreiche Föten. Kulturgeschichtlich bemerkenswerte Funde sind mehrere zu Spielgeräten umgearbeitete Rinderphalangen.

Ressource naturelle aussi précieuse qu’indispensable à nos sociétés contemporaines que pendant l’Antiquité, l’eau est le point central de ce sujet de doctorat, que nous avons décidé d’aborder sous l’angle de sa gestion par les différents échelons du pouvoir au sein de l’Empire romain mais également au niveau individuel et privé, et cela à travers l’exemple de la ville romaine de Dougga, située en Afrique Proconsulaire, l’actuelle Tunisie. Cette problématique propose ainsi de s’intéresser aux moyens mis en œuvre par les différents niveaux de responsabilités dans le but d’approvisionner et de gérer les besoins en eau d’une importante ville romaine d’Afrique, en se consacrant à l’étude des différents types d’installations hydrauliques. Cette recherche permet de distinguer les techniques de construction et d’identifier les savoir-faire locaux et les influences romaines. Enfin, la confrontation des équipements hydrauliques entre les différents types d’édifices, permet d’évaluer le mode de gestion de l’eau à l’échelle de la ville. / Natural resource as precious and indispensable to contemporary society during ancient times, water is the central topic of this thesis. We decided to address in terms of its management by different levels of power within the Roman Empire but also at individual and private, through the example of the provincial city of Thugga, in Africa Proconsularis, now Tunisia. This issue proposes to focus the resources used by the different levels of responsibilities in order to supply and manage the water needs of an important Roman city of Africa, dedicated to the study of different types of hydraulic structures.

MSc (Microbiology) / Department of Microbiology / See the attached abstract below

Worldwide, around 780 million people lack access to improved drinking water sources and 2.5 billion lack improved sanitation (WHO, 2013c). The United Nations (UN) declared access to drinking water and sanitation a fundamental human right in July 2010 (UN, 2010). Each year there are approximately 1.7 billion cases of diarrhoea worldwide. It kills around 2.2 million people globally each year (4.0% of all death), of which 760,000 are children under five years old, mostly in developing countries. Diarrhoeal disease is the second leading cause of death in children under five years old (WHO, 2013c). The lack of drinking water, sanitation facilities and good hygiene practises are considered the main causes for diarrhoea (WHO, 2013d). For this dissertation, a specific health intervention (CBHFA - Community based Health and First Aid) was evaluated which had taken place during three years in 20 target communities in Bokeo Province in Lao PDR. The activities were in the field of water supply, sanitation facilities and hygiene education against diarrhoea. Objective: The objective of this survey study is to show that hygiene behaviour changes in a certain target population through the implementation of CBHFA, if a) individuals have access to improved drinking water sources; b) have access to a HH latrine and c) receive hygiene information. An improvement by 50.0% for each of the hygiene indicator: water use and treatment, hand washing, sanitation, food handling, waste disposal and cleanliness was considered a behavioural change and thus an illustration of the effectiveness of CBHFA. Methods: Hygiene behaviour was measured before and after the CBHFA intervention through above mentioned hygiene indicators. Quantitative figures were collected at two different points in time, in a pre- and post-survey, then analysed and evaluated. Interviews and observations were done through community assessment/evaluation with 20 focus groups in all target communities. 488/487 (pre-survey/post-survey) households (HHs) were interviewed and observed. Knowledge about diarrhoea (definition/signs, prevention, danger, treatment and modes of transmission) was tested. The change in hygiene behaviour as well as the water and sanitation situation was measured through a comparison of the pre- with the post-results. The Relative Risk (RR) and Odds Ratio (OR) were calculated for different variables, such as ethnicity, gender, education, age and income and their changes before and after the implementation of the health intervention project. Results: Water use and sanitation This research clearly shows that the availability of a public water system (gravity fed water system) in the target communities has increased (from 45.0% to 85.0%) as well as the availability and use of hand flush pit latrines (from 20.2% to 63.7%). 95.2% of HHs that owned a latrine used them. The water treatment through “boiling” (from 70.1% to 75.0%) in HHs has improved, but not significantly. Open defecation (OD) decreased from 78.9% to 34.7% (by 56.0%). However it is still practised by more than a third of HHs. OD is one of the highest transmission risks of diarrhoea and can only be eliminated by 100.0% sanitation within the community (WHO, 2008). Hand washing There has been a significant increase of interviewees washing their hands with water and soap (from 8.0% to 38.0%). This result was confirmed by observing the existence of soap in kitchens with 33.0% and in latrines with 20.3% of HHs. The ORs calculated for hand washing “with water and soap” and compared for each stratum of the variables gender, formal education and age group show no statistical difference, but there is a statistical difference regarding income and washing hands “with water and soap”. Relevant occasions for hand washing, such as “after defecation” and “before food preparing”, that could reduce the risk of the transmission of diarrhoeal diseases have not achieved a meaningful improvement. Waste disposal Waste disposal, such as collecting and burning has increased from 23.4% to 42.5% (by 81.6%) according to the HH responses. A matter of concern is the increased HHs (from 26.8% to 29.0%) that disposed their waste by “throwing the waste outside the village”. This waste disposal method is statistically different regarding formal education. The number of HHs with non-educated interviewees where this method was practised has increased over the intervention time in contrast to the number of HHs with educated interviewees. Knowledge about diarrhoea Knowledge was tested. The definition/signs of diarrhoea, its prevention, danger, treatment and modes of transmission were not known by more than 50.0% of the interviewees. It seems that hygiene indicators can improve without this awareness, if improved water and sanitation facilities are provided. However, further investigations are required. The results show that knowledge about diarrhoea is not statistically different regarding formal education and age, but regarding gender. Giving at least one right answer was higher in women than in men. Results and income The results of the pre-survey show that income is significantly associated with owning a latrine and using water vessels, such as pots and jars for drinking, but not associated with using soap for hand washing. However after the implementation of the health interventions, the comparison of the ORs of the HHs with low income per capita (≤200,000 LAK, exchange rate: 1 EUR=10,000 LAK) versus the HHs with high income per capita (>200,000 LAK) shows a statistical difference regarding hand washing “with water and soap”. The OR of the high income per capita group is stronger than the OR of the low income per capita group. Owning a latrine does not show a statistical difference regarding income due to the provision of subsidized latrines by the health intervention project. Occurrence of diarrhoeal diseases The pre-survey results clearly show that diarrhoeal diseases ranked at the top of all recorded cases of diseases. The post-survey results show fewer villages with diarrhoeal outbreak and reduced numbers of reported cases. Summary: An improvement by 50.0% has been reached in hand washing with water and soap, reduction of OD, safe food (by covering), waste disposal (by collecting and burning) and cleanliness but no improvement has been reached in the hygiene indicators “water use and treatment” and in crucial “occasions for hand washing (after defecation, before food preparing)”. Therefore the CBHFA intervention is considered only partially effective in the field of hygiene behaviour change.