The study assessed how stimuli that contradict pre-experimental histories affect the formation of new relations. The study also assessed whether social variables such as race would influence college students’ perceptions of people of different races and job types. Twenty-six college students at a Midwestern University participated in the study. During the pre-test, participants rated the degree of “Good” or “Bad” of various pictures of African American males, police officers, and random objects on a Likert-type scale. Next, based on their pre-test results, participants completed a match to sample task that paired pictures of African American males and police officers opposite of their initial perceptions. Afterward, all participants again completed the Likert-scale rating task. Pre-test results revealed that some participants demonstrated strong negative pre-experimental biases toward police officers and that the race of the participants influenced their pre-test ratings. Individual data showed that 22 out of 24 participants changed their perceptions for at least one stimulus. Match to sample and post-test results revealed that participants with strong pre-experimental biases took more trials to complete the task, scored less accurately when conditions included socially loaded stimuli, and were less likely to change mean ratings for police officers during the post-test rating scale.

Dispersões aquosas do fosfolipídio aniônico DMPG (dimiristoilfosfatidil glicerol) apresentam um comportamento térmico muito peculiar e interessante, principalmente em baixa força iônica. Neste trabalho, mostramos que o DMPG em baixa força iônica apresenta dois regimes com comportamentos térmicos muito distintos, dependendo da concentração lipídica. Acima de uma certa faixa de concentração c\' (c\' = 0,4 ± 0,2 mM), a transição principal gel - cristal-líquido do DMPG não se dá de uma forma brusca, em um intervalo estreito de temperatura, como em muitos dispersões lipídicas, mas apresenta uma região de transição gel - cristal-líquido delimitada por temperaturas aqui denominadas Tmon e Tmoff. Na condição de força iônica mais estudada (tampão Hepes + 2 mM NaCl), Tmon = 17, 5 °C e Tmoff - 35 ºC. Essa região é chamada, no presente trabalho, de fase intermediária, por estar entre as fases gel e fluida, e por conter propriedades específicas, tais como baixo espalhamento de luz e de raio-X de baixo ângulo, e alta condutividade elétrica e viscosidade. A extensão em temperatura da fase intermediária diminui com o aumento da força iônica e concentração lipídica, até que uma temperatura de transição de fase única, centrada em T\"\" é observada. As diversas técnicas utilizadas neste trabalho (espalhamento de luz, calorimetria diferencial de varredura, ressonância paramagnética eletrônica, anisotropia de fluorescência, espalhamento de raio-X de baixo ângulo e microscopia ótica), sugerem que em T mon ocorre um aumento considerável no potencial eletrostático de superfície, levando a um aumento da repulsão entre cabeças polares adjacentes e possivelmente entre vesículas. Esse processo dispararia o início da fusão das cadeias hidrocarbônicas e só seria completado acima de Tmoff. A fase intermediária é caracterizada por um empacotamento não usual da membrana, que pode ser causado pela presença de regiões de altíssima curvatura com grande mobilidade e, possivelmente, com uma maior densidade superficial de cargas. Por outro lado, o comportamento térmico do DMPG abaixo de c\' é bem distinto. A fase intermediária não está presente e o sistema apresenta uma transição de fase centrada em uma única temperatura, Tm. O valor de Tm cresce de 27,5 °C em 0,1 mM DMPG para 41°C em 5 µM DMPG. Um valor tão alto de Tm no regime concentrado foi obtido somente em pH baixo, quando o DMPG encontra-se em seu estado protonado. Foi observado que o valor de T nv contrário ao esperado em dispersões lipídicas carregadas, diminui consideravelmente com o aumento da concentração de NaCl. Propusemos, então, que abaixo de c\' ocorre uma mudança acentuada na superfície da bica ma da, tendo como conseqüência um enorme aumento da afinidade por prótons dos grupos fosfato do DMPG, levando à neutralização completa da superfície lipídica em tomo de 5 µM. Os resultados obtidos no presente trabalho são muito interessantes do ponto de vista do estudo físico-químico de agregados anfifílicos carregados. Além disso, as propriedades do fosfolipídio aniônico DMPG podem ter alguma relevância biológica em domínios específicos de membranas de procariontes. / Aqueous dispersions of the anionic phospholipid DMPG (dimyristoylphosphatidyl glycerol) present a very interesting and peculiar thermal behavior, mainly at low ionic strength. In this work, we show that DMPG at low ionic strength presents two regimes with very different thermal behaviors, depending on the lipid concentration. Above a certain concentration range c\' (c\' = 0,4 ± 0,2 mM), the gel-fIuid main transition of DMPG does not occur in a narrow temperature interval, as shown by many lipid dispersions, but rather presents a gel-fIuid transition region de!imited by temperatures here called Tmon and T moff. At the standard iOIÚC strength condition used (Hepes buffer + 2 mM NaCI), Tmon = 17.5 °C and Tmoff ~35 ºC This region is called intermediate phase in the present work, since it occurs between the gel and fIuid phases, and due to its specific properties, such as low light and small angle X-ray scattering, and high electrical conductivity and viscosity. The temperature extension of the interrnediate phase decreases with the increase in iOIÚC strength and lipid concentration, till a single main phase transition, centered at T DV is observed. The different techniques used in this work (light scattering, differential scanrung calorimetry, electron spin resonance, fluorescence anisotropy, small angle X-ray scattering and optical microscopy), suggest that a considerable increase in the electrostatic surface potential occurs at Tmon, leading to an increase in the repulsion between adjacent headgroups, and possibly between vesicles. This process would trigger the beginrúng of the melting of the hydrocarbon chains, which would finish above TmolE. The intermediate phase is characterized by a non-usual membrane packing, that could be caused by the presence of highly curved regions presenting a loose packing, possibly with higher surface charge density. On the other hand, the thermal behavior of DMPG below é is very different. The intermediate phase is not present and the system displays a main phase transition centered at a unique temperature, Tm The value of Tm increases from 27.5 °C at 0.1 mM DMPG to 41°C at 5 µM DMPC. Such a high T m value was only found in concentrated DMPG dispersion at low pH, when DMPG is in its protonated state. We observed that the T m value, contrary to the expected for charged lipid dispersions, decreases significantly with the increase in NaCI concentration. We propose, then, that below c\' the surface bilayer markedly changes, resulting in a huge increase in the proton affinity to the DMPG phosphate groups, which would lead to a complete neutralization of the lipid surface around 5 µM. The results presented here are very interesting concerning the physical chernistry of charged amphiphilic aggregates. Furthermore, the properties of the anionic phospholipid DMPG might have some biological relevance in specific domains of prokaryotic membranes.

De acordo com dados da Organização Mundial da Saúde (OMS) estimam-se que os agrotóxicos causam anualmente 70 mil intoxicações agudas e crônicas que evoluem para óbito. Dentre eles, o paraquat (PQ) é o que apresenta maior taxa de mortalidade, sendo responsável por cerca de 13% de todos os casos registrados, principalmente devido a falta de um tratamento efetivo. O principal mecanismo de toxicidade proposto está associado ao ciclo redox do PQ, onde ocorre a formação de espécies reativas (ERs) de oxigênio e nitrogênio, levando ao estresse oxidativo (EO). Na literatura há relatos do uso de antioxidantes para casos de intoxicação do PQ. Dessa maneira, nesse trabalho avaliou-se o uso de melatonina associada a nanocápsulas de núcleo lipídico (Mel-LNC) na proteção contra os danos causados pelo PQ, uma vez que o uso da nanotecnologia melhorou a atividade antioxidante dessa molécula. Para tal utilizou-se o sistema in vitro, linhagem celular de adenocarcinoma pulmonar (A549), e o modelo alternativo in vivo, Caenorhabditis elegans. Mel-LNC e nanocápsulas de núcleo lipídico (LNC) foram preparadas de acordo com o método de deposição do polímero pré-formado. Ambas as formulações foram caracterizadas avaliando tamanho de partícula, potencial zeta e pH, e para Mel-LNC foram determinadas a concentração de melatonina e porcentagem de encapsulação. Os resultados encontrados estão de acordo com os parâmetros já validados para essas formulações. Foi possível verificar que as formulações MEL-LNC e LNC se mantiveram estáveis nos meios de cultura utilizados nos ensaios in vitro e in vivo. No estudo in vitro foi observado que o tratamento com ambas as formulações não causaram diminuição da viabilidade nem dano de DNA na linhagem celular utilizada. Além disso, foi verificado a internalização da Mel-LNC utilizando-se a formulação marcada com rodamina B, sendo possível verificar uma intensa fluorescência vermelha ao redor do núcleo da célula. O pré-tratamento com Mel-LNC foi capaz de aumentar a viabilidade celular e diminuir o dano oxidativo de DNA causado pelo paraquat após 24 horas de exposição, porém isso não ocorreu quando as células foram pré-tratadas com melatonina livre. No estudo com o modelo alternativo C. elegans, foi utilizada uma formulação de Mel-LNC marcada com rodamina B (Mel-LNC-RoB), a fim de verificar a absorção dessa formulação pelo nematoide. Foi possível observar que a internalização da Mel-LNC no C. elegans ocorre principalmente pela via oral, uma vez que se verificou uma intensa fluorescência no intestino do nematoide após o tratamento com a Mel-LNC-RoB e após três horas, essa fluorescência se distribuiu pelo restante do corpo, apresentando inúmeros pontos de fluorescência fora do intestino. Com relação à avaliação do efeito protetor nesse modelo alternativo in vivo, pode-se inferir que o pré-tratamento com Mel-LNC aumentou a sobrevida, diminuiu a produção de espécies reativas (ERs) e manteve o desenvolvimento normal dos nematoides após a exposição ao PQ, sendo que isso não foi verificado quando os mesmos foram pré-tratados com melatonina livre. Além disso, verificou-se que as nanocápsulas de núcleo lipídico (LNC) são seguras para o uso no modelo C. elegans, uma vez que apresentou alto valor para a dose letal 50 (DL50), e alterações no desenvolvimento e produção de ERs somente ocorreram em doses mais elevadas que as utilizadas em nossos experimentos. Dessa maneira, a formulação de Mel-LNC mostrou-se um promissor candidato para estudos futuros nos casos de intoxicação por paraquat. / According to estimations by World Health Organization (WHO), pesticides are responsible for 70 thousand acute intoxication cases that lead to death per year. Among these compounds, paraquat (PQ) presents the highest mortality rate, about 13% of all registered cases, especially for the lack of effective treatment. The major mechanism of toxicity proposed is associated to its redox cycle, in which oxygen and nitrogen reactive species (RS) are generated culminating in oxidative stress (OS). Some reports in the literature support the use of antioxidants for PQ intoxication cases. The present study aimed to evaluate the use of melatonin-loaded lipid-core nanocapsules (Mel-LNC) in the protection against PQ-induced damages, considering that nanotechnology has improved the antioxidant activity of this molecule. For this purpose, an in vitro system composed by lung adenocarcinoma (A549) cell line, and the in vivo alternative model of Caenorhabditis elegans have been utilized. Mel-LNC and unloaded lipid-core nanocapsules were prepared by self-assembly and characterized by particle sizing, zeta potential and pH, and for Mel-LNC formulation it was determined the drug content and encapsulation efficiency. The results are in agreement with the parameters already validated for these formulations. It was possible verify that Mel-LNC and LNC formulations remained stable in the culture medium utilized in in vitro and in vivo experiments. Results from in vitro studies showed that none of the formulations induced reduction in cell viability or DNA damage in treated cells. Besides, it was observed the internalization of Mel-LNC marked with rhodamine B, showing an intense red fluorescence around the cell nucleus. Pretreatment with Mel-LNC was able to enhance cell viability and diminish DNA oxidative damage caused by paraquat after 24h exposure, which could not be observed when cells were pretreated with Mel. In the study with the alternative model C. elegans, a rhodamine (Ro)-linked Mel-LNC formulation was prepared in order to assess the absorption of the formulation by the nematode. Mel-LNC uptake in C. elegans was found to occur mainly by the oral route, once an intense fluorescence was observed in the intestine after treatment with Mel-LNC-RoB, which after 3h distributed to the rest of the body, presenting numerous fluorescence dots outside the intestine. In relation to the evaluation of protection with the in vivo alternative model, results indicate that pretreatment with Mel-LNC increased survival rate, reduced the production of reactive species and maintained the normal development of nematodes after paraquat exposure, while the same observations were not found after pretreatment with free melatonin. In addition, the lipid-core nanocapsules (LNC) were found to be safe in the C. elegans model, due to its high lethal dose (LD50) value, and development alterations and RS production only occurred in the higher doses than those utilized in our experiments. Therefore, the Mel-LNC formulation demonstrated to be a promising candidate for future studies aiming treatment of paraquat intoxication cases.

Full scale lateral load testing was performed on four 12.75x0.375 pipe piles spaced at 3.9, 2.9, 2.8, and 1.7 pile diameters behind an MSE wall which was constructed for this research to determine appropriate reduction factors for lateral pile resistance based on pile spacing behind the back face of the wall. The load induced on eight soil reinforcements located at various transverse distances from the pile and at different depths was monitored to determine the relationship between lateral load on the pile and load induced in the reinforcement. Each pile was loaded towards the wall in 0.25 in. increments to a total deflection of 3.0 in. Additionally, wall panel displacement was also monitored to determine if it remained in acceptable bounds. The results of the research indicate that pile resistance tends to decrease as spacing decreases. P-multipliers for the 3.9, 2.9, 2.8, 1.7D tests were found to be 1.0, 1.0, 1.0, and 0.5, respectively using back-analysis with the computer model LPILE. However, these multipliers are higher than expected based on previous testing and research. Piles spaced further than 3.8D can be assumed to have no interaction with the wall. The resistance of piles spaced closer to the wall than 3.8D can be modeled in LPILE using a p-multiplier less than 1.0. The reinforced backfill can be modeled in LPILE using the API Sand (1982) method with a friction angle of 31º and a modulus of approximately 60 pci when a surcharge of 600 psf is applied. If no surcharge is applied, a friction angle of 39º and modulus of 260 pci is more appropriate. Maximum wall panel displacement was highest for the 2.8D test and was 0.35 in. at 3.0 in. of pile head displacement. For all the other tests, the maximum wall displacement at 3.0 in. of pile head displacement was similar and was approximately 0.15 inches. Induced load in the soil reinforcement increases with depth to the 2nd or 3rd layer of reinforcement after which it decreases. Induced load in the reinforcement increases as pile spacing decreases. Induced load in the reinforcement decreases rapidly with increased transverse distance from the pile. Induced load in the reinforcement can be estimated using a regression equation which considers the influence of pile load, pile spacing behind the wall, reinforcement depth or vertical stress, and transverse spacing of the reinforcement.

Abstract available in the Annual coaches and Sport Science College.

Anti ram perimeter barriers are part of the protection of important facilities such as power plants, air ports and embassies against unrestricted vehicle access. Many different systems can be used to achieve the containment goal. One of these systems makes use of soil embedded posts either single posts if the soil is hard enough or groups of soil embedded posts tied together by beams if the soil is not hard enough for a single post to stop the in-coming truck. The design of these soil embedded posts needs to take account a number of influencing factors which include the soil strength and stiffness, the post strength and stiffness, the mass of the vehicle and its approach velocity. This dissertation describes the work done to develop a set of design recommendations to select the embedment of a single post or group of posts. The post is a steel beam with an H shape cross section: W14X109 for the single post system and W14X90 for the group system with a double beam made of square hollow steel section HSS8X8X1/2. The spacing of the posts for the group includes 2.44 m, 4.88 m, and 7.32 m. The soil strength varies from loose sand and soft clay to very dense sand and very hard clay. The vehicle has a mass of 6800 kg and the velocities include 80 km/h, 65 km/h, and 50 km/h. The design guidelines presented here are based on 10 medium scale pendulum impact tests, 2 medium scale bogie impact tests, 1 full scale impact test on a single post, 1 full scale impact test on a group of 8 side by side posts with a 5.2 m spacing and connected with two beams, approximately 150 4-D numerical simulations of full scale impact tests using LS-DYNA, as well as fundamental theoretical concepts.

This thesis presents a novel monolithic phased array implemented using the RF MEMS technology. The structure, which is designed at 15 GHz, consists of four linearly placed microstrip patch antennas, 3-bit distributed RF MEMS low-loss phase shifters, and a corporate feed network. The RF MEMS phase shifter employed in the system consists of three sections with a total of 28 unit cells, and it occupies an area of 22.4 mm &amp / #61620 / 2.1 mm. The performance of the phase shifters is improved using high-Q metal-air-metal capacitors in addition to MEMS switches as loading elements on a high-impedance coplanar waveguide transmission line. The phased array is fabricated monolithically using an in-house surface micromachining process, where a 1.2-&amp / #61549 / m thick gold structural layer is placed on a 500-&micro / m thick glass substrate with a capacitive gap of 2 &amp / #61549 / m. The fabrication process is simple, requires only 6 masks, and allows the implementation of various RF MEMS components on the same substrate, such as RF MEMS switches and phase shifters. The fabricated monolithic phased array occupies an area of only 6 cm &amp / #61620 / 5 cm. The measurement results show that the phase shifter can provide nearly 20&amp / #61616 / /50&amp / #61616 / /95&amp / #61616 / phase shifts and their eight combinations at the expense of 1.5 dB average insertion loss at 15 GHz. The phase shifters can be actuated with 16 V, while dissipating negligible power due to its capacitive operation. It is also shown by measurements that the main beam can be steered to 4&amp / #61616 / and 14&amp / #61616 / by suitable settings of the RF MEMS phase shifters.

The bandwidth-enhancement characteristics of slot-loaded rectangular microstrip antennas constructed on a thin microwave substrate have been investigated in this dissertation. The primary design process about this topic is demonstrated. Firstly, a new antenna design idea is provided from pre-determined antenna design specifications or obtained antenna performances from the previous antenna design, which lead to a novel antenna configuration. From the results of the simulation software IE3DTM, the characteristics of this new antenna configuration are obtained and compared with the experimental results. The antenna configuration is also modified to achieve a final optimal design from the comparison results. The study of the single-feed dual-frequency rectangular microstrip antenna with a pair of bent slots is first presented. By embedding properly-designed slots on a rectangular microstrip patch, the impedance characteristics of this antenna design have been effectively changed to exhibit dual-resonant behavior, which result in the excitation of two adjacent resonant modes with similar radiation characteristics. Furthermore, the two resonant modes can be excited at frequencies very close to each other to form a wider operating bandwidth by embedding additional perturbation slots. Four successful antenna designs with different embedded-slot shapes for bandwidth enhancement have been implemented and discussed in this dissertation.

The recent improvement of additive manufacturing has allowed designers to achieve a level of complexity and customizability that is difficult or impossible to accomplish using traditional manufacturing processes. As a result, much research has been conducted on developing new methods to utilize the larger design space brought by additive manufacturing. One such research area is in the design of mesoscale lattice structures. Mesoscale lattice structures are a type of cellular structure with support element sizes on the order of magnitude of centimeters. These types of structures are engineered for high performance and have applications in industries where both low weight and high strength are desired. However, due to the small size of their struts, these structures can easily have hundreds to thousands of individual struts. As a result, design poses a unique challenge. Current methods approach design of mesoscale lattice structures as a topological optimization problem, treating each strut diameter in the structure as a design variable. For structures with a fewer number struts, these optimization methods can converge, but will generally be very time-consuming. For structures with a large number of struts, the optimization problem becomes too large for current algorithms to solve. In previous research, a new, highly efficient design method for mesoscale lattice structures was presented that eliminates the need for global size or topological optimization. This method, termed the Size, Matching and Scaling method, used a unique combination of a solid-body finite element analysis and a library of pre-defined lattice configurations, termed the "unit-cell library," to generate lattice topologies. The results from this method were highly promising: design time was significantly reduced when compared to optimization methods. Furthermore, lattices designed using the SMS method had performance results that were either comparable or better than their optimized counterparts. However, the method developed was highly conceptual, lacking a true systematic methodology for generating topologies and suffering from some gaps in implementation. In this research, we present a modified Size Matching and Scaling (SMS) design method. Firstly, we introduce and outline the modified methodology. This methodology particularly includes an optimization step for determining strut diameters that replaces the manual search used in the original method. Secondly, we expand and explore the unit-cell library in an attempt to improve the performance of lattices generated using the SMS method. In particular, we optimize several unit-cell configurations and compare their performance in the context of the SMS method. Finally, we test the updated SMS methodology and unit-cell library using various design examples. Results from the various example problems indicate that optimization is not only a viable systematic method for determining diameter values, but is actually preferred to the manual, iterative process used in the original method. Furthermore, various optimization algorithms and approaches yield different results. Between the two optimization algorithms utilized in this method: constrained optimization and least-squares minimization, constrained minimization converges faster, but least-squares minimization yields slightly improved performance results. In addition to these algorithms, a one-variable approach using an untested, simplifying assumption, dubbed the "28% approach," was tested. Results indicate that this assumption was incorrect and cannot be utilized. Finally, results from the expanded unit-cell library indicate that the best unit-cell configuration is still the same original unit-cell configuration utilized in the first SMS method. The addition of more unit-cell does not improve the performance of structures generated using the SMS method. In fact, both performance and design time worsen when additional configurations are utilized.

Mobile offshore drilling units are being used in the Gulf of Mexico to produce oil and gas. Anchoring systems such as drag embedment anchors and vertically loaded anchors are used to keep these units in place. Past mooring system failures due to hurricanes in 2004 and 2005 initiated a need to better understand the performance of these anchors to in-plane and out-of-plane loading conditions. In-plane and out-of-plane loading cause the anchor to translate or rotate in the directions of its six degrees of freedom. Behavior and holding capacity of the anchors when loaded in each of is six degrees of freedom are important in understanding and predicting their behavior. An experimental program was devised to investigate the behavior of anchors in pure translation and rotation loading. The scaled-model anchors were embedded at a measured depth in a soil bed of clay with an undrained shear strength between 10 and 20 psf and then loaded to failure. A rotation testing frame was designed to impose rotational loading in the yaw, roll and pitch directions. Test results from the experimental program are consistent and repeatable. The bearing factors for pure bearing fell well within the range of existing experimental and analytical studies on simple plates. Bearing factors for in-plane and out-of-plane shear and for all rotations are higher than those for simple plates due to presence of the shank. When the resistance is normalized by area of the fluke, the wider model provide greater normalized resistance to yawing, similar normalized resistance to pitching and rolling and less normalized resistance to bearing and shearing. It was concluded that the holding capacity of an anchor in its six degrees of freedom depends largely on its geometry, including the fluke and the shank. / text