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No description available.

Magister Scientiae (Medical Bioscience) - MSc(MBS) / Recently nanoparticles (NPs) have been introduced and used in combination with therapeutic approaches to develop nanotechnology-enabled medicine. These nanostructures allow for the exploitation of the physiochemical properties which may be beneficial in cancer treatment. The use of NPs in nanomedicine has proven successful in modern chemotherapeutics and has demonstrated promising potential in in vivo and in vitro radiosensitization studies. This is a baseline study aimed to determine the cytotoxic effects of AuNPs for potential radiosensitization analysis. The study analysed the effects of different AuNP sizes (30, 50 and 80nm), concentrations (5, 10 and 15 μg/ml) over various time periods in CHOK1 and A549 cells.

No description available.

The increasing role of sustainability will lead the mobility by road into a more efficient and interactive system between infrastructure, environment and vehicles. The expand of emobility based on Electric Vehicles has been restricted by numerous shortcomings such as their driving range, the battery size, the dependence on charging stations and the time required for its charging. One of the solutions to overcome these limitations is can be the construction of electrified roads. The study compares the most significant opportunities for eRoads as the solutions ofInductive Power Transfer (IPT), conductive track and pantograph in climate change aspect.This thesis is also intended to describe these systems, as they represent different ways for reach electrification of ordinary roads. IPT is a contactless solution which uses the well-known electromagnetic induction principle. Pantograph and conductive track are both conductive solutions. In the first case, it is an overhead solution and for the track, the energy is transferred to the vehicles from a continuous rail embedded in the pavement. The aim of the study is to develop an open and transparent Life Cycle Assessment (LCA)framework for electrified roads that could be used for decision support. The main objective is to build a cradle-to-grave LCA model in SimaPro for an electrified highway asphalt road in Sweden. / A fenntarthatóság, fenntartható fejlődés világszerte egyre fontosabb szerephez jut,elősegítve ezáltal azt, hogy hagyományos útburkolatok helyett hatékonyabb, interaktív rendszerek kerüljenek kiépítésre. Ezek az „okos” utak összekapcsolják az infrastruktúrát, akörnyezetet és az embert, mint járművezetőt. Jelenleg, a már hagyományosnak mondható e-közlekedésnek – mely az elektromos autók használatán alapul – számos hátrányafigyelhető meg. Többek között a korlátozott vezetési távolság és akkumulátor méret,valamint a töltési lehetőségektől és töltésidőtől való függés. Ezek leküzdésének egyik módja lehet a villamosított utak építése és elterjedése. A tanulmány célja egy nyílt, átlátható és könnyen kezelhető életciklus-elemzés (LCA) keretrendszer kiépítése a villamosított utak számára, mint döntéstámogató rendszer ajövőbeli beruházásokhoz. Ez egy svédországi villamosított autópálya, SimaPro programbanlétesített életciklus-elemzés modell építésével kerül bemutatásra. A dolgozat a három legjelentősebb típusú rendszereket hasonlítja össze a klímaváltozást befolyásoló hatások tekintetében. Ezek az indukció elvén alapuló IPT, a vezető pálya és a pantográf megoldások. A tanulmány szintén hivatott bemutatni a rendszereket, minthogyezek merőben különböző elveken alapuló megoldásokkal érik el a hagyományos utakvillamosítását. Míg elektro-mágneses töltés elvén alapuló rendszer a kontakt nélküli megoldások közé tartozik, addig a másik két rendszer álladó fizikai kapcsolatot igényel a jármű és a töltési rendszer elemei között.

High-resolution physically ordered gene maps for the horse (Equus caballus, ECA) are essential to the identification of genes associated with hereditary diseases and traits of interest like fertility, coat color, and disease resistance or susceptibility. Such maps also serve as foundations for genome comparisons across species and form the basis to study chromosome evolution. In this study seven equine chromosomes (ECA6, 7, 10, 15, 18, 21 and X) corresponding to human chromosomes (HSA) 2, 19 and X were selected for high-resolution mapping on the basis of their potential involvement in diseases and conditions of importance to horses. To accomplish this, gene- and sequence-specific markers were generated and genotyped on the TAMU 5000rad horse x hamster RH panel. Additionally, screening of a BAC library by overgoes and subsequent STS content mapping and fingerprinting approaches were used to assemble and verify a BAC contig along a ~5 Mb span on ECA21. Dense gene maps were generated for each of the seven equine chromosomes by adding 408 new markers (285 type I and 123 type II) to the current maps of these chromosomes, thereby greatly improving overall map resolution to one mapped marker every 960kb on average (range: 700 kb – 1.3 Mb). Moreover, the contig on ECA21 contained 47 markers (42 genes and 5 microsatellites) as well as 106 STS markers distributed along 207 BAC clones. Comparisons of these maps with other species revealed a remarkably high level of horse-human X chromosome conservation, as well as two evolutionary breakpoints unique to Perissodactyls or Equids for the equine homologues of HSA19 and HSA2, one of which has been more precisely localized by the ECA21 contig. Thus, high resolution maps developed for these chromosomes i) provide a basis to map traits of interest rapidly to specific chromosomal regions, ii) facilitate searches for candidate genes for these traits by fine comparisons of the equine regions with corresponding segments in other species, and iii) enable understanding the evolution of the chromosomes. Expansion of this work to the entire equine genome will be important for developing novel strategies for diagnosis, prevention, and treatment of equine diseases.

No description available.

We have developed a system to hunt and reuse special gene integration sites that allow for high and stable gene expression. A vector, named pRGFP8, was constructed. The plasmid pRGFP8 contains a reporter gene, gfp2 and two extraneous DNA fragments. The gene gfp2 makes it possible to screen the high expression regions on the chromosome. The extraneous DNA fragments can help to create the unique loci on the chromosome and increase the gene targeting frequency by increasing the homology. After transfection into Chinese hamster ovary cells (CHO) cells, the linearized pRGFP8 can integrate into the chromosome of the host cells and form the unique sites. With FACS, 90 millions transfected cells were sorted and the cells with strongest GFP expression were isolated, and then 8 stable high expression GFP CHO cell lines were selected as candidates for the new host cell. Taking the unique site created by pRGFP8 on the chromosome in the new host cells as a targeting locus, the gfp2 gene was replaced with the gene of interest, human ifngamma, by transfecting the targeting plasmid pRIH-IFN. Then using FACS, the cells with the dimmest GFP fluorescence were selected. These cells showed they had strong abilities to produce the protein of interest, IFN-gamma. During the gene targeting experiment, we found there is positive correlation between the fluorescence density of the GFP CHO host cells and the specific production rate of IFN-gamma. This result shows that the strategy in our expression system is correct: the production of the interesting protein increases with the increase fluorescence of the GFP host cells. This system, the new host cell lines and the targeting vector, can be utilized for highly expressing the gene of interest. More importantly, by using FACS, we can fully screen all the transfected cells, which can reduce the chances of losing the best cells. / Singapore-MIT Alliance (SMA)

Control of gene expression is essential for synthetic biology. Drug-inducible systems allow for gene expression levels to be precisely regulated through the administration of exogenous chemical inducers, and by combining these systems, more complex circuits with multiple inputs and outputs can be designed. However, the number of existing orthogonal drug-inducible systems is limited. Therefore, there remains a need for new, small-molecule-inducible systems that are orthogonal to both existing systems and native cellular function. Here, we focus on the development and applications of two small-molecule inducible systems for use in basic research, synthetic systems, and therapeutics: the ligand-inducible connection (LInC) system and the HaXS8-inducible system. In the LInC system, the NS3/4a protease from hepatitis C virus is used to regulate the linkage between genetically fused DNA-binding elements and transcriptional effector domains, which remain linked only in the presence of an NS3/4a protease inhibitor. The antiviral drugs which are used as inhibitors for NS3-based systems provide ideal small-molecule inputs for synthetic biology applications, as they are designed to be orthogonal to native biological function and have been tested for clinical use in humans. We demonstrate the construction and validation of a LInC transcription factor (TF) system, as well as further extension of the system with an “all-in-one” single vector design. We then continue on to describe the application of a small molecule (HaXS8) heterodimer system based on the SNAP-tag and HaloTag domains to control gene expression and protein behavior. We demonstrate the construction and validation of several HaXS8-inducible proteins, including TFs, Cre recombinase, and caspase-9. Together, our work on these two systems provides additional orthogonal methods of small molecule-inducible gene expression and protein function for use in control of cellular behavior. / 2024-05-23T00:00:00Z

Diss. Karlskrona : Blekinge tekniska högskola, 2008.