According to a study of the European Commission, approximately one million hips are replaced by prostheses worldwide every year. The interaction of the human body with foreign materials that are subjected to alternating mechanical load in a highly corrosive environment still provides challenges. The main factors affecting prosthesis failure are stress shielding effect and poor osseointegration. In this thesis the problem of prosthesis failure has been approached from the material and from the osseointegration point of view trying to give a global solution to the problem. Niobium and hafnium, which are demonstrated to be totally biocompatible, were used to design a Ti-based alloy. The effect of the alloying elements regarding microstructure and elastic modulus was studied and the best composition was deeply characterized in terms of microstructure, elastic modulus, corrosion resistance and superficial energy. Recombinant fragments of fibronectin were synthesised spanning the cell attachment site and the heparin binding domain which are important for cell viability. These motifs were used to functionalise the surface of the TiNbHf alloy. Two tethering methods were studied: physisorption and silanisation. Silanisation was not used before to immobilise fibronectin recombinant fragments onto metallic substrates and in this thesis, its good performance was demonstrated. In vitro studies were made with each fragment and with different combinations of the fragments, which showed the importance of the heparin binding domain to obtain a cell response equivalent to that of fibronectin in terms of cell adhesion, proliferation and differentiation. / De acuerdo con un estudio de la Comisión Europea, aproximadamente un millón de caderas son remplazadas por prótesis en el mundo anualmente. La interacción del cuerpo humano con materiales externos sujetos a una carga mecánica alternante en un medio altamente corrosivo todavía presenta ciertos desafíos. Los factores que contribuyen principalmente al fallo de una prótesis son el apantallamiento de cargas y la pobre osteointegracion. En la presente tesis el problema de la fallida de prótesis ha sido abordado desde el punto de vista del material y de la osteointegracion en un intento de dar una solución global al problema. El niobio y el hafnio, cuya total biocompatibilidad ha sido demostrada, se han utilizado para diseñar una aleación de titanio. El efecto de dichos aleantes respecto a la microestructura y el módulo elástico ha sido estudiado y la mejor composición ha sido profundamente caracterizada en términos de microestructura, módulo elástico, resistencia a la corrosión y energía superficial. Fragmentos recombinados de fibronectina han sido sintetizados abarcando la zona de adhesión celular y la unión de heparina, las cuales son esenciales para la viabilidad celular. Dichos motivos han sido utilizados para funcionalizar la superficie de la aleación TiNbHf. Dos métodos de unión diferentes han sido estudiados: fisisorción y silanización. La silanización es un método que no se ha utilizado hasta el momento para inmovilizar fragmentos de fibronectina sobre superficies metálicas y en la presente tesis su idoneidad ha sido demostrada. Finalmente, estudios celulares in vitro se han llevado a cabo con cada fragmento y con diferentes combinaciones de ambos, lo cual ha mostrado la importancia de la zona de unión de heparina para obtener una respuesta celular equivalente a la obtenida con la molécula de fibronectina en cuanto a adhesión celular, proliferación y diferenciación.

En esta tesis se mostra como la aminocatalisis pueda ser utilizada para resolver importantes problemas en sintesis organica. En el primer proyecto tres reacciones en cascada aminocatalizadas han sido optimizadas para la síntesis de productos spiro-cíclicos derivados de las benzofuranonas. Estas moléculas, que tienen un esqueleto presente también en muchos productos naturales, han sido sintetizadas como un sólo enantiomero gracias al uso de amina primaria y secondaria. Un segundo proyecto muestra la primera activación aminocatalizada de cetonas α-substituidas α,β-insaturadasen el ámbito de una reacción de adición de sulfa-Michael, in esto contexto ha sido posiblemodificar la selectividad de la diastereoselección del catalizador a través de simples modificaciones en las condiciones de reacción. Finalmente, se muestra el primer empleo de aminas secundarias quirales en la funcionalizaccion directa de aldehídos α-substituidos α,β-insaturados. El sistema se ha empleado en una reaccion aldolica (addiccion) y una alquilacion (sostitucion) vinylogas. / In this thesis it is shown how aminocatalysis can be employed to solve different problems in organic synthesis. In a first project three complementary cascade reactions have been optimized for the synthesis of spirocylcic products bearing the benzofuranone moiety. The products were synthetized has single enantiomer exploiting the ability of both primary and secondary aminocatalysts to orthogonally activate ketones and aldehydes. In a second project is reported the first use ofα-substitutedα,β-unsaturated ketonesin aminocatalysisfor developing an asymmetric sulfa-Michael addition reaction. In addition, it was possible to modulate the enforced sense of diastereoselectivity of the catalyst by simply modifying the reaction conditions. Finally we could use, for the first time, chiral secondary amines to promote the dienamine activation of α-branched enals. The catalytic system has been used to realize two enantioselectivetrasformations, a vinylogousaldol and a -alkylation reactions.

The following essay aims to examine the use of lyrical themes linked to religion. The music styles in focus in this study are death metal and melodic death metal in Sweden. The study is based on 14 Swedish bands that are or have been active during the last 30 years. In this study I ask which themes connected to religion are used in the lyrics from the middle of the 80’s till today and how the use of themes changes during this time period.       My hypothesis was that since metal’s function and purpose is to break boundaries and test the social limits the lyrics inevitably must change over time to continue to draw attention and provoke. The lyrics have been studied with qualitative text analysis and put in a timeline divided in four eras to see the changes over time and how they are expressed. Each era is divided into different categories and themes that are found in the lyrics.       This study shows that the lyrics in the chosen genres connected to religion change over time, in accordance with my hypothesis. The main lyrical themes are Satanism, Anti-religion and religion critics, Christian themes, Esoterism and mysticism and Mythology. The lyrical themes have changed from dealing primarily with Satanism and anti-Christianity/religion in the 80’s and early 90’s to more complex lyrics about esoterism, mysticism, spiritism, and mythology today.

xxiii, 507 p. / This dissertation contextualizes melodic variation within a cultural, historical, and cognitive framework. This work discusses how traditional musicians learn how to vary melodies by observing norms of social and musical behavior exhibited by senior musicians. The core of this dissertation is the transcription and analysis of fifty source recordings of fifty different Irish musicians playing one tune each dating from between 1904 and 2007. Though the transcriptions of the recordings exhibited a high instance of melodic variation (48.2% of the measures), only a small percentage of variation fell on set accented tones (an average of 7.3%). The considerable invariance of set accented tones suggests that part of what constitutes the concept of a tune in an Irish musician's mind relates to the pitch of these key tones. I introduce the term aesthetic conservatism to designate a philosophical approach to performance practice that seeks to maintain both the dance genre and tune identity. I argue that aesthetic conservatism may be a by-product of archetypes and exemplars created through transcriptions and recording technology. This conservatism may also be a function of famine-induced fear of cultural dissolution or inferiority with respect to more prominent music-making supercultures. I call on the philosophy of aesthetic conservatism to explain why few set accented tones are varied. Of the measures that were varied, 74% of those variations involved the addition, subtraction, or redistribution of ornamentation. To catalogue the variety of variations within this sample, I propose a taxonomy that is designed to account for the number of notes in a measure and to assess intervallic differences over successive repetitions of a tune. Finally, I propose a theory to explain the cognitive processes that allow a musician to vary a melody. I suggest that in the mind of a traditional musician there is both a tune schema and a variation schema. These are flexible models that are distinct and separate but that interact within a short span of time because of the exceptionally efficient anatomy of a musician's brain. / Committee in charge: Anne Dhu McLucas, Chairperson; Lori Kruckenberg, Member; Stephen Rodgers, Member; Glen Waddell, Outside Member

AN ABSTRACT OF THE THESIS OF Linnea Anderson, for the Masters of Science degree in Communication Disorders and Sciences, presented on 4/3/2013, at Southern Illinois University Carbondale. TITLE: The Use of Melodic Intonation Therapy in the Clinical Setting MAJOR PROFESSOR: Kenneth O. Simpson Melodic Intonation Therapy (MIT) is a popular published program for Broca's aphasia. This investigation focused on the clinical use of MIT. Five participants were selected to take part in a survey. Of these five, none had purchased the program and all had created a modified MIT program. Some of the participants used their modified MIT program with individuals who do not have Broca's aphasia. Varying levels of effectiveness were indicated by the participants about their modified MIT program.

This master’s degree project optimized the synthesis route to a functional lactide by increasing the total yield from 25 to 33 % and reducing the number of unit operations from 17 to 10. This was done by optimizing an existing synthetic pathway to better fit larger scale manufacturing. The monomer was also successfully copolymerized with ʟ-lactide and functionalized by attaching poly(ethylene glycol) units of varying chain lengths to the polylactide chain, which gives some antifouling properties to the copolymer. The resulting material, a functionalized polylactide, is an interesting material suitable for the use in medical implats, largely thanks to its versatility and ability to be tailored for a specific purpose. / I detta examensarbete optimerades en syntesväg till en funktionaliserad laktid genom att öka utbytet från 25 till 33 % samt minska antalet enhetsoperationer från 17 till 10 st. Detta gjordes genom att anpassa en befintlig syntesväg till tillverkning i större skala. Monomeren sampolymeriserades även med ʟ-laktid och funktionaliserades genom att polyetylenglykol av varierande kedjelängd fästes till polylaktidkedjan, vilket gav en hämmande effekt på celladheransen till materialets yta. Det resulterande materialet: En funktionaliserad polylaktid, är ett intressant material som lämpar sig för användning i medicinska implantat, mycket tack vare sin mångsidighet och möjligheten att utforma materialen för specifika ändamål.

The aim of this work was to functionalize silicon dioxide surfaces with biochemical molecules in such a way that biorecognition of target molecules in solution will be possible. By introducing a tool set of different molecules and characterization methods, a more universal approach towards various biosensor setups is presented. This includes on the one hand preparation of the biosensor surfaces to allow further molecule attachment via their reactive functional groups. Secondly, the selection of chemical molecules providing suitable counterparts for abundant functional groups of potential receptors is discussed. Two detection schemes are introduced – based on an antibody to detect the antibiotic amoxicillin and aptamers to detect thrombin. The antibody was implemented in an inverse competition assay to probe such small target molecules. Antibiotic residues are often present in wastewater. Aptamers, so-called artificial antibodies, were selected as they provide many advantages over antibodies. As a model system, two different thrombin binding aptamers were chosen which allowed to perform sandwich assays as well. The protein thrombin plays an important role in the blood coagulation cascade. To probe the individual modification steps, different techniques for analysis were applied. Surface micropatterning was introduced to improve recognition of modified areas and fluorescence-to-background ratios resulting in a thrombin detection limit down to 20 pM. One important goal was the integration in ion-sensitive field-effect transistor devices. Aptamers are small in size which might enable a higher sensitivity of these devices compared to the use of antibodies because of the Debye layer thickness. As a final step, first measurements towards silicon nanowire based field-effect transistor biosensors were carried out on devices with bottom-up and top-down fabricated nanowires using both proposed receptor-analyte combinations. The potential of these devices as portable sensors for real-time and label-free biosensing is demonstrated. / Ziel dieser Arbeit war es Siliziumdioxidoberflächen so mit biochemischen Molekülen zu funktional- isieren, dass die biologisch spezifische Erkennung von Zielmolekülen in Lösung möglich wird. Hier wird eine Auswahl an geeigneten Molekülen und Charakterisierungsmethoden für einen vielseitigen Ansatz gezeigt, der auf verschiedene Biosensorsysteme anwendbar ist. Das beinhaltet zum Einen die Präparation der Biosensoroberflächen, so dass die Moleküle über reaktive funktionelle Gruppen angebunden werden können. Als zweites ist die Auswahl der chemischen Moleküle wichtig, da diese die passenden Gegenstücke zu potentiellen funktionellen Gruppen der Rezeptoren darstellen. Zwei verschiedene Detektionsvarianten werden eingeführt – Antikörper gegen das Antibiotikum Amoxicillin und Aptamere gegen Thrombin. Der Antikörper wurde in einen inversen Wettbewerbsassay integriert um einen solch kleinen Ana- lyten detektieren zu können. Rückstände von Antibiotika sind häufig in Abwässern zu finden. Ap- tamere, sogenannte künstliche Antikörper, weisen gegenüber Antikörpern viele Vorteile auf. Als ein Modellsystem wurden zwei unterschiedliche Thrombin bindende Aptamere verwendet, was auch die Durchführung von Sandwich Assays ermöglichte. Das Protein Thrombin spielt eine wichtige Rolle bei der Blutgerinnung. Um die einzelnen Modifikationsschritte zu untersuchen, wurden verschiedene Charakterisierungsmethoden angewendet. Die Mikrostrukturierung der Funktionalisierung erleichterte die Erkennung der modifizierten Flächen und verbesserte das Fluoreszenz-zu-Hintergrund Verhältnis. Das führte zu einer Detektionsgrenze von 20 pM für Thrombin. Ein wichtiges Ziel dieser Arbeit war die Integration der Funktionalisierung in einen ionen-sensitiven Feldeffekttransistor. Die kleinen Aptamere könnten dabei aufgrund der geringen Debye-Schichtdicke bei diesen Sensoren eine höhere Sensitivität als mit Antikörpern ermöglichen. Zuletzt wurden erste Messungen hin zu Silizium Nanodraht basierten Feldeffekttransistor Biosen- soren mit beiden untersuchten Rezeptor-Analyt-Kombinationen durchgeführt. Sowohl die Chips mit bottom-up als auch mit top-down gewachsenen Nanodrähten zeigen dabei ihr Potential als handliche Sensoren zur markerfreien Detektion in Echtzeit.

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