Urban greenspace has an important role in supporting biodiversity and providing structural and functional connectivity between natural habitats. However, the mapping of vegetation in urban areas presents challenges, as urban vegetation is highly fragmented and heterogeneous. This study compared maps with respect to their strengths and weaknesses in providing ecologically relevant information in an urban area with the purpose to find how useful they are for local planning. The study took place in the urban part of Dún Laoghaire-Rathdown County, Ireland. The maps analysed were the Urban Atlas 2012 provided by Copernicus, the Prime 2 database provided by the Ordnance Survey Ireland, and two maps that were generated by this project from Sentinel 2 satellite imagery, one which showed vegetation based on calculated NDVI and one with four land cover classes calculated with a supervised classification. These maps were compared from three points of view: correspondence of vegetation classes, structural connectivity, and quality of vegetated areas. Analyses of structural connectivity were based on several landscape metrics, one of them was the degree of coherence (Cm) which reflects how connected the vegetation patches are in the landscape. Definition of quality was based on contextual indicators, such as the proximity to streams and areas with high conservation value, and vegetation present on a historical map from the 1830s. The results showed that the overlap of vegetation between the datasets varies between 27.0-89.1%. The different datasets definitions of vegetation affect how well they correspond in terms of where vegetation can be found. Resolution is also an important factor, as urban vegetation patches tend to be small and thus a coarse minimum mapping unit – as is the case for the Urban Atlas – masks important information on the configuration of vegetated areas in the urban area. The structural connectivity of vegetation differed little between Prime 2 (Cm = 7.95×10-2%) and the Urban Atlas (Cm = 5.87×10-2%). However, the distance between vegetated patches was on average shorter in Prime 2. This suggests that the Prime 2 dataset, because of its higher spatial resolution, contains more information on potential stepping stones for species to move around the landscape. The distribution of vegetated areas with higher contextual quality was mainly close to the boundaries of the urban area. Connectivity in a fragmented landscape like this urban study area plays a crucial role in maintaining populations of flora and fauna. It is therefore important to consider in the management of urban vegetation and in planning for development. This study offers a first insight in the structural connectivity of Dún Laoghaire-Rathdown County, which can be used to make more informed decisions that will sustain urban biodiversity.

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Pas de résumé / No abstract

Protein therapeutics offer numerous advantages over small molecule drugs and are rapidly becoming one of the most prominent classes of therapeutics. Unfortunately, they are delivered almost exclusively by injection due to biological obstacles preventing high bioavailability via the oral route. In this work, numerous approaches to overcoming these barriers are explored. PH-Responsive poly(itaconic acid-co-N-vinylpyrrolidone) (P(IA-co-NVP)) hydrogels were synthesized, and the effects of monomer ratios, crosslinking density, microparticle size, protein size, and loading conditions were systematically evaluated using in vitro tests. P(IA-co-NVP) hydrogels demonstrated up to 69% greater equilibrium swelling at neutral conditions than previously-studied poly(methacrylic acid-co-N-vinylpyrrolidone) hydrogels and a 10-fold improvement in time-sensitive swelling experiments. Furthermore, P(IA-co-NVP) hydrogel microparticles demonstrated up to a 2.7-fold improvement in delivery of salmon calcitonin (sCT) compared to methacrylic acid-based systems, with a formulation comprised of a 1:2 ratio of itaconic acid to N-vinylpyrrolidone demonstrating the greatest delivery capability. Vast improvement in delivery capability was achieved using reduced ionic strength conditions during drug loading. Use of a 1.50 mM PBS buffer during loading yielded an 83-fold improvement in delivery of sCT compared to a standard 150 mM buffer. With this improvement, a daily dose of sCT could be provided using P(IA-co-NVP) microparticles in one standard-sized gel capsule. P(IA-co-NVP) was also tested with larger proteins urokinase and Rituxan. Crosslinking density provided a facile method for tuning hydrogels to accommodate a wide range of protein sizes. The effects of protein PEGylation were also explored. PEGylated sCT displayed lower release from P(IA-co-NVP) microparticles, but displayed increased apparent permeability across a Caco-2 monolayer by two orders of magnitude. Therefore, PEG-containing systems could yield high bioavailability of orally delivered proteins. Finally, a modified SELEX protocol for cellular selection of transcellular transport-initiating aptamers was developed and used to identify aptamer sequences showing enhanced intestinal perfusion. Over three selection cycles, the selected aptamer library showed significant increases in absorption, and from an initial library of 1.1 trillion sequences, 5-10 sequences were selected that demonstrated up to 10-fold amplification compared to the naïve library. These sequences could provide a means of overcoming the significant final barrier of intestinal absorption. / text

A Lagrangian finite-element model of the plunge phase of the friction stir welding process was developed to better understand the plunge. The effects of both modeling and experimental parameters were explored. Experimental friction stir plunges were made in AA 7075-T6 at a plunge rate of 0.724 mm/s with spindle speeds ranging from 400 to 800 rpm. Comparable plunges were modeled in Forge2005. Various simulation parameters were explored to assess the effect on temperature prediction. These included the heat transfer coefficient between the tool and workpiece (from 0 to 2000 W/m-K), mesh size (node counts from 1,200 to 8,000), and material model (five different constitutive relationships). Simulated and measured workpiece temperatures were compared to evaluate model quality. As spindle speed increases, there is a statistically significant increase in measured temperature. However, over the range of spindle speeds studied, this difference is only about 10% of the measured temperature increase. Both the model and the simulation show a similar influence of spindle speed on temperature. The tool-workpiece heat transfer coefficient has a minor influence (<25% temperature change) on simulated peak temperature. Mesh size has a moderate influence (<40% temperature change) on simulated peak temperature, but a mesh size of 3000 nodes is sufficient. The material model has a high influence (>60% temperature change) on simulated peak temperature. Overall, the simulated temperature rise error was reduced from 300% to 50%. It is believed that this can be best improved in the future by developing improved material models.