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Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraeaElison Kalman, Grim January 2019 (has links)
This report summarizes the work on the cloning, expression, and purification of PerR, a metal sensing regulator from Saccharopolyspora erythraea and the subsequent characterization using small angle X-ray scattering and other biochemical methods. The report aims to provide an insight into prokaryotic metal homeostasis, provide a better understanding of how PerR works and provide valuable information for the continued work on the crystallization of PerR.
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