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1	Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraea Elison Kalman, Grim January 2019 (has links) This report summarizes the work on the cloning, expression, and purification of PerR, a metal sensing regulator from Saccharopolyspora erythraea and the subsequent characterization using small angle X-ray scattering and other biochemical methods. The report aims to provide an insight into prokaryotic metal homeostasis, provide a better understanding of how PerR works and provide valuable information for the continued work on the crystallization of PerR. structural biology cell culture protein expression genetic engineering spectroscopy X-ray crystallography small angle X-ray scattering SAXS transcription factor PerR metal binding antibiotics Saccharopolyspora erythraea S. erythraea soil bacterium actinobacteria actinomycetes oxidative stress peroxide stress structure determination characterization stabilization peroxide sensor iron sensor manganese sensor metalloprotein metal ion homeostasis metal ion prokaryotic strukturbiologi cellodling proteinuttryck genteknik spektroskopi röntgenkristallografi småvinkel-röntgenspridning SAXS transkriptionsfaktor PerR metallbindande antibiotika Saccharopolyspora erythraea S. erythraea jordartsbakterie Aktinobakterier Actinobacteria Actinomycetes oxidativ stress peroxid stress strukturbestämning karaktärisering stabilisering peroxidsensor järnsensor mangansensor metalljonshomeostas metalljoner prokaryot Structural Biology Strukturbiologi

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Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraea