No description available.

Prenatal exposure to Zika virus (ZIKV) can result in microencephaly and congenital Zika syndrome but why some brain cells and structures are initially spared by the virus is unknown. Here, a novel murine model of ZIKV infection incorporating in utero electroporation with cell type specific promotors was used to identify the time course of ZIKV infection and to determine which neural precursor cells are initially infected or spared. In vivo time course studies revealed early presence of ZIKV in apical radial glial cells (aRGCs) while infection of basal intermediate progenitor cells climbed after three days of virus exposure. ZIKV-exposed fetal brains exhibited microencephaly as early as 1 day post injection, caused by apoptosis and reduced proliferation, and this change in brain size persisted until birth regardless of developmental age at infection. During infection, 60% of aRGC basal fibers were perturbed while 40% retained normal morphology, indicating that aRGCs are not uniformly vulnerable to ZIKV infection. To evaluate this heterogeneous vulnerability, we generated cell type-specific fate mapping plasmid probes using a previously published single cell RNA-Seq dataset on the E15.5 mouse neocortical wall. The results indicate that one class of aRGC preferentially expresses the putative ZIKV entry receptor AXL, and that these cells are more vulnerable to ZIKV infection than the other aRGC subtypes with low AXL expression. Together, these data highlight important temporal and cellular details of ZIKV fetal brain infection and may be important for prevention strategies and for management of congenital Zika syndrome.

No description available.

INTRODUÇÃO: Houve uma epidemia de infecção pelo vírus Zika (ZIKV) no Brasil, entre 2015 e 2016, que refletiu no aumento de casos de microcefalia neonatal secundária à infecção congênita pelo ZIKV. Para compreensão do padrão de acometimento neurológico dessas crianças foi realizado um projeto de pesquisa pelo Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (HCFMRP-USP), conhecido como ZIG, e realizado seguimento neurológico desses pacientes por meio do Exame Neurológico de Hammersmith Neonatal (HINE-N). OBJETIVOS: Avaliar pacientes com microcefalia secundária à infecção congênita pelo ZIKV por meio do HINE- N, descrever as características clínicas e de neuroimagem dos pacientes estudados e identificar fatores pré-natais, perinatais e demográficos potencialmente relacionados ao grau de comprometimento do exame neurológico. METODOLOGIA: O presente estudo faz parte do Núcleo de Estudos sobre Infecção Materna, Perinatal e Infantil (NEIMPI) do HCFMRP-USP e trata-se de um estudo não experimental, transversal, com intuito de correlacionar variáveis qualitativas e quantitativas. Participaram do estudo crianças com microcefalia secundária à infecção congênita pelo ZKV nascidas no município de Ribeirão Preto e seu Departamento Regional de Saúde (DRS XIII), no período de outubro de 2015 a dezembro de 2016. Os dados foram coletados no período de outubro de 2015 a janeiro de 2017. Realizou-se avaliação do perímetro craniano (PC), aplicação do HINE e coleta de dados em prontuário. Os dados foram processados e analisados com a utilização do Pacote Estatístico Statistical Package for the Social Sciences (SPSS), versão 22.0. RESULTADOS: Foram avaliadas 18 crianças, sendo predomínio do sexo feminino (55,6%), nascidas a termo, com exceção de uma que nasceu com 32 semanas, maioria originária de Ribeirão Preto (66,7%), via de parto normal (72,2%), ocorrido no Centro Obstétrico do HCFMRP - USP (83,3%), todas as crianças tiveram APGAR maior que 7. O peso de nascimento foi entre 1228 e 3200 gramas, com PC entre 26 e 31,5 cm. 72,2% das crianças fizeram ultrassom transfontanela e 100% fizeram ressonância magnética de encéfalo, com associação entre os resultados encontrados neles (ambos com p<ou=0,001). O score HINE-N foi baixo, porém não foram encontradas associações entre o score e as variáveis clínicas (ambos com p>0,05). Evidenciou-se associação entre a realização de pré-natal e a cidade de residência, local de nascimento, diagnóstico de microcefalia e de infecção pelo ZIKV no pré-natal, tipo de parto (p< ou = 0,001). A medida do PC apresentou associação direta com a presença de crise convulsiva (p<0,05) e houve associação entre crise convulsiva, ventriculomegalia e calcificação (p< ou= 0,001). CONCLUSÃO: O padrão de acometimento neurológico das crianças com microcefalia secundária à infecção congênita pelo ZIKV é muito severo, com presença de sinais precoces de paralisia cerebral desde o exame neonatal. Este estudo sugere seguimento prospectivo desses pacientes para melhor avaliação prognóstica e associação do score HINE com condições clínicas pré e perinatais / INTRODUCTION: There was an epidemic of Zika virus infection (ZIKV) in Brazil between 2015 and 2016, which reflected the increase in cases of neonatal microcephaly secondary to congenital ZIKV infection. A study was carried out for the Hospital das Clínicas of the University of São Paulo at Ribeirão Preto Medical School (HCFMRP-USP), and the followup of these patients was performed using the Neonatal Hammersmith Neurological Exam (HINE-N) aiming to understand the neurological involvement pattern of these children. OBJECTIVES: To evaluate patients with microcephaly secondary to ZIKV congenital infection using HINE-N, to describe the clinical and neuroimaging characteristics of the patients studied, and to identify prenatal, perinatal and demographic factors potentially related to the degree of impairment of neurological examination. METHODOLOGY: This study is part of the Center for Studies on Maternal, Perinatal and Child Infection (NEIMPI) of the HCFMRP-USP. It is a non-experimental, cross-sectional study aimed at correlating qualitative and quantitative variables. Children with microcephaly secondary to congenital ZKV infection born in the city of Ribeirão Preto and its Regional Health Department (DRS XIII), from October 2015 to December 2016, participated in the study. Data were collected in the period of October 2015 to January 2017. An evaluation of the cranial perimeter (PC), the application of HINE and data collection in medical records were performed. Data were processed and analyzed using the Statistical Package for Social Statistical Package (SPSS), version 22.0. RESULTS: A total of 18 children were evaluated, being 55,6% female, all to term with the exception of one who was born at 32 weeks, most of them from Ribeirão Preto (66.7%), normal delivery (72.2%), occurred in the Obstetric Center of the HCFMRP - USP (83.3%), all children had APGAR greater than 7. The birth weight was between 1228 and 3200 grams, with PC between 26 and 31.5 cm. 72.2% of the children had transfontanel ultrasonography and 100% had brain magnetic resonance imaging, with an association between the results found in them (both with p <or = 0.001). The HINE-N score was low, but no associations were found between the score and the clinical variables (both with p> 0.05). It was evidenced an association between prenatal and city of residence, place of birth, diagnosis of microcephaly and ZIKV infection in the prenatal period, type of delivery (p <or = 0.001). The PC measurement presented a direct association with the presence of seizures (p <0.05) and there was an association between seizure, ventriculomegaly and calcification (p <or = 0.001). CONCLUSION: The pattern of neurological involvement of children with microcephaly secondary to congenital ZIKV infection is very severe, with early signs of cerebral palsy since the neonatal examination. This study suggests a prospective follow-up of these patients for better prognostic evaluation and association of the HINE score with pre and perinatal clinical conditions

O Zika vírus (ZIKV) é um flavivírus transmitido pelo mosquito Aedes aegypti e que se espalhou rapidamente pelas Américas, causando uma epidemia no Brasil em 2015 . Um número crescente nos casos de infecções veio acompanhado de um aumento no número de fetos e bebês nascidos com microcefalia, levando a um chamado de emergência mundial de saúde. Históricamente, o ZIKV não havia causado infecções de destaque em humanos e a reermegência dessa ameça viral associada à defeitos do nascimento foi logo relacionada à evolução e consequente distinção entre os genótipos virais, o original Zika africano e seu descendente Zika asiático, que chegou ao Brasil. A hipótese da cepa brasileira do ZIKV ser a causadora de microcefalia e de outros defeitos do nascimento ganhou mais respaldo após a identificação do vírus em amostras de tecido cerebral e líquido amniótico de fetos. Posteriormente, a associação direta entre microcefalia e a síndrome congênita com o ZIKV foi confirmada por meio da aplicação de modelos biológicos experimentais que se revelaram susceptíveis à infecção viral, como células do sistema nervoso central em sistemas 2D e 3D in vitro e camundongos prenhês. Esse trabalho teve como objetivo investigar a infecção da cepa brasileira do ZIKV (ZIKVBR) em diferentes células humanas neurais in vitro diferenciadas a partir de células-tronco pluripotentes induzidas, além de criar uma plataforma para teste de fármacos in vitro contra o vírus. Nossos resultados comprovaram a susceptibilidade e permissividade celular à infecção do ZIKVBR em células neuronais e, em especial, progenitoras neurais, causando morte celular por apoptose. Além disto, quando células progenitoras neurais foram cultivadas em suspensão, formando neuroesferas, o ZIKVBR foi capaz de causar uma redução na população de células, gerando uma anormalidade morfológica semelhante à microcefalia. Além do mais, quando células progenitoras neurais infectadas com ZIKVBR foram diferenciadas em neurônios maduros, a análise da sinaptogênese revelou que esses neurônios apresentavam uma menor densidade de puncta sináptica, indicando um comprometimento no funcionamento das sinapses que pode estar contribuindo para os problemas associados com a síndrome congênita do ZIKV. Por fim, o tratamento dessas células com a droga Sofosbuvir, um inibidor de RNA polimerase dependente de RNA aprovado para uso clínico, foi capaz de resgatar NPCs e neurônios apoptóticos. Em suma, nossos dados indicam que o ZIKVBR infecta preferencialmente células progenitoras neurais, replicando-se eficientemente e causando morte por apoptose nessas células e neurônios maduros diferenciados de células progenitoras neurais infectadas apresentam uma menor desidade de puncta sináptica. Finalmente, a reutilização de compostos farmacêuticos já aprovados para uso clínico pode acelerar o tratamento para indivíduos infectados pelo ZIKV onde a prevenção já não é mais opção, como no caso de mulheres grávidas. / Zika virus (ZIKV) is a mosquito-borne flavivirus transmitted by Aedes aegypti that has rapidly spread through the Americas, causing a widespread epidemic in Brazil in 2015. A increasing number of infection cases was followed by a rise in the number of fetuses and babies born with microcephaly, leading to a global health emergency call. Up to then, ZIKV had not caused meaningful infections in humans and the reemergency of this viral threat associated with birth defects was soon related to viral genotype mutations and its consequent distinction from the original african Zika strain to its descendent asian Zika strain, which reached Brazil. The hypotesis of the brazilian ZIKV strain being responsible for microcephaly and other birth defects gained support after the isolation and identification of the virus in samples of cerebral tissue and amniotic fluid of fetuses. Subsequently, the direct association between microcephaly and congenital syndrome with ZIKV was confirmed through the application of biologic experimental models which proved susceptible to viral infection, as for cells from the central nervous system cultured in 2D and 3D models as well as pregnant mice. The aim of this study was to investigate the brazilian ZIKV strain (ZIKVBR) infection in different human neural cells in vitro differentiated from induced pluripotent stem cells, as well as creating a platform for in vitro drug testing with antiviral capabilities. Our results showed cellular infection susceptibility and permissiveness to ZIKVBR in neurons and, specially, neural progenitor cells, displaying cell death by apoptosis. Futhermore, when neuronal progenitor cells cultured in suspension, forming neurospheres, were infected with ZIKVBR, it caused a reduction in cell population, displayed by evident morphological abnormalities resembling to microcephaly. Additionally, when neural progenitor cells infected with ZIKVBR were diferentiated further into mature neurons, synaptogenesis analysis revealed these neurons displayed fewer synaptic puncta density, indicating a compromise in synapse functioning that may be contributing to problems associated with ZIKV congenital syndrome. Moreover, cell treatment with Sofosbuvir, a RNA polymerase RNAdependent inhibitor approved for clinical use, was able to rescue apoptotic NPCs and neurons. In summary, our results reveal that ZIKVBR preferentially infects neural progenitor cells, efficiently replicating itself and causing death by apoptosis in these cells and mature neurons differentiated from infected neural progenitor cells display reduced synaptic puncta density. Lastly, the repurpose of FDA approved compounds may aid in accelerating treatment for infected individuals whose prevention is no longer an option, as it is for pregnant women.

Brain development is an exquisitely coordinated process of progenitor cell proliferation followed by the migration of progeny to their final location in the developing brain. There are a myriad of points at which this process can be disturbed, and the examination of these perturbations help us further understand basic science, as well as epidemics sweeping through the world around us. Microcephaly, which is defined as a head circumference greater than 2 standard deviations below the mean, can occur through genetic, infectious, vascular, or metabolic etiologies, and the studies herein examine two forms by which microcephaly occurs. First, we investigate the role of the dynein regulatory protein Nde1 in the development of the neocortex, which is the outer region of the forebrain. NDE1 mutations are associated with severe microcephaly, and we find that unlike most microcephaly genes whose products have one role in the cell cycle, Nde1 is required at three discrete points in neuronal progenitors, termed radial glia progenitors (RGPs). We initially find that Nde1 is required to recruit dynein to the nuclear envelope to allow for interkinetic nuclear migration (INM) during G2. Additionally, Nde1 helps to initiate primary cilia resorption at the G1-to-S transition. Finally, there is a necessity for Nde1 at the G2-to-M transition after the completion of INM and prior to nuclear envelope breakdown. These three distinct roles for Nde1 illustrate the breadth of functions that the protein has during RGP proliferation, and help to explain why patients with NDE1 mutations have such severe microcephaly. As this work was ongoing there was a global outbreak of a new pathogen that had previously been dormant throughout Africa and Asia, only to emerge at epidemic proportions in the Western Hemisphere. This pathogen, the Zika Virus (ZIKV), is particularly alarming because of its subclinical course in adults but devastating consequences for fetal development, with the hallmark symptom being microcephaly. Using our organotypic brain slice model system, we demonstrate the ability of a variety of ZIKV isolates to infect and replicate in embryonic brain tissue. All ZIKV isolates that infect the organotypic slices lead to increases in apoptosis, though these increases are particularly pronounced in isolates from the Asian/American lineages. Notably, one isolate from a patient in Nigeria (termed 30656) does not replicate in mouse neuronal tissue, but electroporation of the 30656 ZIKV genome allows for a single cycle replication, suggesting that this isolate is unable to enter RGPs. All infectious isolates are pathogenic in early- and mid- gestation embryonic tissue, but only one isolate infects and replicates in late- gestation embryonic tissue. This was the most recently isolated sample tested, and it demonstrates a predilection for neurons, suggesting that ZIKV may be mutating as it spreads. These results provide foundational insight into the pathogenesis of ZIKV- associated microcephaly, and illustrate how studies of genetic forms of microcephaly can enhance and facilitate our understanding of infectious causes of the disease.

Submitted by admin tede (tede@pucgoias.edu.br) on 2018-04-17T17:46:02Z No. of bitstreams: 1 ALYNE APARECIDA FERREIRA FREITAS.pdf: 750774 bytes, checksum: f4eb9631ba2e7c7ff4dcdee8b5719580 (MD5) / Made available in DSpace on 2018-04-17T17:46:03Z (GMT). No. of bitstreams: 1 ALYNE APARECIDA FERREIRA FREITAS.pdf: 750774 bytes, checksum: f4eb9631ba2e7c7ff4dcdee8b5719580 (MD5) Previous issue date: 2018-03-05 / The objective of this study was to evaluate the family impact in parents of children diagnosed with microcephaly by Zika virus. This is a cross-sectional analytical study with a quantitative approach, using a sociodemographic questionnaire and family impact scale. A total of 76 parents with children undergoing rehabilitation and rehabilitation treatment were surveyed at a reference center in Goiânia. After the application of the instruments, a database was made using the IBM SPSS Statistics 18 software. Descriptive analyzes were performed using frequency, mean and standard deviation. The tests used to evaluate the existence or not of a statistically significant difference (p≤0.05) between independent and multiple variables were the Student's T-Test and the ANOVA Scheffé test, respectively. The mother is the main caregiver, the majority of whom are young mothers, divorced or divorced, with a corresponding monthly family income of 1 to 3 minimum wages, belonging to the low income class. It was observed that the predominant period of the diagnosis of the microcephalic child was in prenatal care. The greater the tendency to engage in activities with friends, parties and to go to bars, the individuals were more likely to perform physical and leisure activities. It was evidenced a difficulty on the part of the parents to find reliable persons to take care of the child, as well as, lack of understanding of other people for the burden that is to take care of the deficient son and expressed the desire in not having more children. It is concluded that after the initial shock of receiving the diagnosis of the child, the parents go through the reorganization phase, adapting to the challenges, changes in routine and family structure. The involvement of health professionals is essential, providing support and guidance to these families. Coping strategies emphasized social support in the institutional network, optimism, resilience and spirituality. / O objetivo deste estudo foi avaliar o impacto familiar em pais de crianças diagnosticadas com microcefalia pelo Zika vírus. Trata-se de um estudo transversal analítico com abordagem quantitativa, utilizando-se de um questionário sociodemográfico e a escala de impacto familiar (EIF). Foram pesquisados 76 pais com filhos em tratamento de reabilitação e readaptação em um centro de referência de Goiânia/Go. Após a aplicação dos instrumentos, foi confeccionado um banco de dados utilizando o software IBM SPSS Statistics 18. Por meio deste, foram realizadas análises descritivas utilizando-se frequência relativa e absoluta, média e desvio padrão. Os testes utilizados para avaliar a existência ou não de diferença estatisticamente significativa (p≤0,05) entre amostras independentes e múltiplas variáveis, foi utilizado o teste de análise de variância (ANOVA) Scheffé. A mãe é a principal cuidadora, sendo na sua maioria mães jovens, divorciadas ou desquitadas, apresentando renda mensal familiar correspondente de 1 a 3 salários mínimos, pertencentes à classe renda baixa. Observou-se ser no pré-natal o período predominante do recebimento do diagnóstico do filho microcefálico. Quanto maior a tendência de empreender atividades com amigos, festas e a frequentar bares, os indivíduos se mosraram mais propensos a realizarem atividades físicas e de lazer. Foi evidenciado uma dificuldade por parte dos pais em encontrar pessoas de confiança para cuidar do filho, bem como, falta de compreensão de outras pessoas pelo fardo que é cuidar do filho deficiente e expresso o desejo em não ter mais filhos. Conclui-se que após o choque inicial do recebimento do diagnóstico do filho, os genitores passam pela fase de reorganização, adaptando aos desafios, alterações na rotina e estrutura familiar. É fundamental o envolvimento dos profissionais de saúde, fornecendo suporte e orientação a essas famílias. Destacaram-se como estratégias de enfrentamento o apoio social na rede institucional, otimismo, resiliência e espiritualidade.

Human genetics has identified essential roles for many centriole- and cilia-related proteins during human development. Mutations in centrosome-associated genes commonly cause microcephaly, or "small brain," and mutations in cilia-associated genes cause a diverse spectrum of diseases termed "ciliopathies." However, the functional relationships between these two crucial organelles are less well studied. The activities of centrosome-related proteins during mitosis and cytoskeletal remodeling are well-characterized, but their in vivo functions are incompletely understood. Here, we identify novel human mutations in a centrosomal gene which encodes a regulatory subunit of a microtubule interacting protein, and uncover unexpected pathways during vertebrate development. Human mutations cause severe microlissencephaly, reflecting defects in cerebral cortical neurogenesis, and loss of function in mice and zebrafish confirm essential roles in embryonic development, neurogenesis, and cell survival. Surprisingly, null mutant embryos display hallmarks of aberrant Sonic hedgehog signaling, including holoprosencephaly. Deficient induced pluripotent stem cells and lymphoblasts show defective proliferation and spindle structure, while deficient fibroblasts also demonstrate a remarkable excess of centrioles, including excessive maternal centrioles, with supernumerary cilia but deficient Hedgehog signaling. Our results reveal novel roles for this protein in regulating overall centriole number, mother centriole and cilia number, and as an essential gene for normal Hedgehog signaling during neocortical development.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-04-03T14:49:52Z No. of bitstreams: 1 HaryelleNarymaConfessorFerreira_DISSERT.pdf: 903183 bytes, checksum: 512cec0622808aed442e5d8b7f4b59a6 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-04-10T20:46:08Z (GMT) No. of bitstreams: 1 HaryelleNarymaConfessorFerreira_DISSERT.pdf: 903183 bytes, checksum: 512cec0622808aed442e5d8b7f4b59a6 (MD5) / Made available in DSpace on 2018-04-10T20:46:08Z (GMT). No. of bitstreams: 1 HaryelleNarymaConfessorFerreira_DISSERT.pdf: 903183 bytes, checksum: 512cec0622808aed442e5d8b7f4b59a6 (MD5) Previous issue date: 2018-02-26 / Introduction: The increase in the number of cases of microcephaly in Brazil and its association with the Zika virus (ZIKV) is a global public health problem. The International Classification of Functioning Disability and Health (ICF) model is a powerful tool and extremely relevant in managing disability. Objective: Describe the functioning profile of children with microcephaly associated with ZIKV in two states of northeastern Brazil. Methods: This is a descriptive cross-sectional study. The sociodemographic characteristics, head circumference and other clinical data were collected from medical charts, physical examinations, measuring instruments and interviews with the children and their parents. The Brazilian Portuguese version of the ICF core set for cerebral palsy (CP) was used. Each ICF category was assigned a qualifier, which ranged from 0 to 4 (no disability, mild disability, moderate disability, severe disability and complete disability). For environmental factors, 0 represents no barrier and 4 total barrier; +0, no facilitator +4, total facilitator. Results: A total of 34 children with microcephaly caused by ZIKV were recruited (18 girls and 16 boys) at four rehabilitation facilities in Rio Grande do Norte and Para?ba states, Brazil. The average age of the participants was 21 months and head circumference z-scores ranged from 0.92 to -5.51. The functioning profile revealed complete disability in most of the body function categories (b). The activity and participation areas (d) were highly impacted, particularly in mobility-related categories. With respect to environmental factors (e), most of the sample reported a total facilitator for the nuclear family, friends and health services, systems and policies, as well as a total barrier to social attitudes. Conclusion: This is the first study that describes the functioning profile of children with microcephaly associated with ZIKV, using a tool based on the ICF in Brazil. Our findings reinforce the need to maximize health care and access to information ? based on the ICF ? for multiprofessional teams, administrators, family members and children.

Introduction: Microcephaly is a multifactorial malformation that is manifested by an inadequate development of the child's brain. This condition leads to motor and sensory disturbances that may have varied impairment. Rehabilitation cares for these children aim to minimize harms and promote harmony in the development of their organic functions. Culturally, family care is provided mainly by mothers, especially when there is specificity in this care, a condition that entails increasing daily tasks. The routine of consultations for diagnosis, treatment and rehabilitation may be associated with changes in the quality of life, since these mothers can give up their lives at the expense of child care. Caring for mothers of children with microcephaly is essential, since they are their main caregivers, can favor the continuity of these children's treatments and reduce family damage. Objective: to evaluate the quality of life of mothers of children with microcephaly compared to the quality of life of mothers with children of the same age but with normal neuropsychomotor development (NPM). Method: this study was a cross-sectional, comparative, analytical study held in a public reference service. Seventy-eight (78) mothers with children between birth and two years old, with and without changes in their NMP, were interviewed. The abbreviated Questionnaire for the Evaluation of Quality of Life (WHOQOL-bref) and a sociodemographic evaluation questionnaire, developed by the author, were used. The data were analyzed descriptively, evaluating the association between variables and correlation tests. Results: mothers of children with microcephaly showed lower scores for various quality of life domains, however there was only a statistically significant difference for the environmental domain (48.40 for the group of mothers of children with microcephaly vs. 57.13 for the group of mothers with children with normal NPM, P<0.02). It should be noted that there were also significant negative correlations between the majority obstetric variables, maternal age and quality of life scores. There was no significant association between the child’s age and such scores. Conclusion: the mothers of children with microcephaly were mostly residents of the interior and showed deficiency in some factors related to basic health needs. The fact that the children with neuropsychomotor variations have not influenced their mother’s quality of life, rather, the mother’s quality of life is affected predominantly by housing conditions and financial resources. / Introdução: a microcefalia é uma malformação de causa multifatorial que se manifesta por um desenvolvimento inadequado do cérebro da criança. Essa condição acarreta distúrbios motores e sensoriais que podem ter comprometimento variado. O cuidado com essas crianças é voltado à reabilitação, a fim de minimizar danos e promover harmonia no desenvolvimento de suas funções orgânicas. Culturalmente, o cuidado familiar é prestado principalmente pelas mães, sobretudo quando há especificidade nesse cuidado, condição que acarreta no aumento das tarefas diárias. A rotina de consultas para diagnóstico, tratamento e reabilitação pode estar associada a alterações da qualidade de vida, pois essas mães podem abdicar da sua vida em detrimento do cuidado com a criança. Cuidar de mães de criança com microcefalia é essencial, visto que são suas principais cuidadoras, pode favorecer a continuidade dos tratamentos dessas crianças e redução dos danos familiares. Objetivo: Avaliar a qualidade de vida de mães de crianças com microcefalia, reconhecer as características obstétrico-ginecológicas e sócio-demográficas dessas mães e comparar os resultados com as mães de crianças sem comprometimento neuropsicomotor. Método: estudo transversal, comparativo, analítico e quantitativo, realizado em um serviço ambulatorial público de referência para atendimento de crianças com microcefalia em Sergipe. Foram entrevistadas 78 mães de crianças de zero a dois anos, com e sem alterações no desenvolvimento neuropsicomotor (DNPM) . Será considerado grupo G1 mães de crianças com microcefalia e o grupo G2 mães de crianças com intolerância/alergia à lactose. Utilizou-se o Questionário de Avaliação de Qualidade de Vida Abreviado (WHOQOL-bref) e o questionário de avaliação sociodemográfica, desenvolvido pelos autores. A associação entre variáveis categóricas foi avaliada por meio dos testes de Qui-quadrado e Exato de Fisher e a associação entre variáveis quantitativas por meio dos testes de correlação de Spearman e Pearson. Para verificar as diferenças existentes entre os grupos foram utilizados os testes de Mann-Whitney U e T de Student independente. Resultados: mães de crianças com microcefalia apresentaram menores escores de qualidade de vida dos domínios e total, porém houve diferença estatisticamente significativa apenas para o domínio ambiental, (48,40) para o grupo de mães de crianças com microcefalia, (57,13) para o grupo de mães de crianças com DNPM normal (p<0,02). Cabe ressaltar que foram observadas correlações negativas significativas entre a maioria das variáveis gineco-obstétricas, idade materna e escores de qualidade de vida. A idade da criança não apresentou associação significativa com tais escores. Conclusão: as mães de crianças com microcefalia em sua maioria eram moradoras do interior e demonstravam carência em alguns fatores relacionados a necessidades básicas de saúde. O fato de as crianças terem desenvolvimento neuropsicomotor alterado não influenciou na qualidade de vida das mães, acredita-se que está mais associada às condições de moradia e recursos financeiros. / Aracaju, SE