The well-known symbol for conservation, the giant panda bear, and the original red panda have been forced into remote habitats due to anthropogenic disturbance, making ecological study difficult. Therefore the first known species distribution model was created to predict the most likely areas of occurrence within the known range of these elusive animals. These models were then projected onto North America and evaluated against existing breeding programs. Additionally, the close proximity of the Gray Fossil Site and the discovery of the most complete fossil red panda specimens in the world allowed ecomorphological comparisons between the modern red panda, Ailurus fulgens and the fossil red panda, Pristinailurus bristoli. Spatial data and morphometric results from this study will aid conservation work and breeding programs globally.

Despite being separated by millions of years of evolution, black bears (Ursus americanus) and brown bears (Ursus arctos) can be difficult to distinguish based on skeletal and dental material alone. Complicating matters, some Late Pleistocene U. americanus are significantly larger in size than their modern relatives, obscuring the identification of the two bears. In the past, fossil bears have been identified based on differences in dental morphology or size. This study used geometric morphometrics to look at overall differences in cranial shape and used step-wise discriminant analysis to identify specific characters that distinguish cranial morphology between black and brown bears. Such differences could prove important in identifying fossil bears when crania are present but teeth are missing. Furthermore, being able to properly identify U. arctos and U. americanus crania is important in understanding evolutionary and ecological distinctions among both fossil and modern bears. Principal components, discriminant, and thin plate spline analyses indicated a clear morphological separation between the crania of U. americanus and U. arctos and highlighted key identifying features including a more convex forehead and a narrower, more elongate rostrum in U. arctos than U. americanus.

Analysis of surface models reconstructed from human MR images gives re- searchers the ability to quantify the shape and size of the cerebral cortex. Increasing the reliability of automatic reconstructions would increase the precision and, therefore, power of studies utilizing cortical surface models. We looked at four different workflows for reconstructing cortical surfaces: 1) BAW + LOGIMSOS- B; 2) FreeSurfer + LOGISMOS-B; 3) BAW + FreeSurfer + Machine Learning + LOGISMOS-B; 4) Standard FreeSurfer(Dale et al. 1999). Workflows 1-3 were developed in this project. Workflow 1 utilized both BRAINSAutoWorkup(BAW)(Kim et al. 2015) and a surface reconstruction tool called LOGISMOS-B(Oguz et al. 2014). Workflow 2 added LOGISMOS-B to a custom built FreeSurfer workflow that was highly optimized for parallel processing. Workflow 3 combined workflows 1 and 2 and added random forest classifiers for predicting the edges of the cerebral cortex. These predictions were then fed into LOGISMOS-B as the cost function for graph segmentation. To compare these work- flows, a dataset of 578 simulated cortical volume changes was created from 20 different sets of MR scans. The workflow utilizing machine learning (workflow 3) produced cortical volume changes with the least amount of error when compared to the known volume changes from the simulations. Machine learning can be effectively used to help reconstruct cortical surfaces that more precisely track changes in the cerebral cortex. This research could be used to increase the power of future projects studying correlations between cortical morphometrics and neurological health.

Background: Nonsyndromic cleft lip and palate (NSCL/P) is a complex trait caused by genetic and environmental factors that interact producing a wide spectrum of orofacial malformations, including dental anomalies. The underlying genetic etiology that accounts for phenotypic variation in affected families is poorly understood. Purpose: The purpose of this study is to utilize shape and microesthetic analysis to characterize the maxillary anterior dentition in unaffected parents of children with NSCL/P (cases) compared to control adults with no CL/P history to identify dental morphology features that are part of the NSCL/P phenotypic spectrum and can therefore be used in refining NSCL/P phenotypes and identifying genetic risk factors. Methods: Individuals were recruited from 5 sites including Iowa, Texas, Hungary, the Philippines, and Pittsburg, PA. From a total of 3202 individuals, 420 quailified after strict selective criteria. Digital photographs from 198 cases and 222 controls were analyzed using linear metrics and 2D-coordinate landmark-based geometric morphometrics (GM) to compare dental esthetics and deviations from golden proportions." Differences in central incisor and connector height proportions were evaluated using paired T-tests. Anterior tooth shapes were examined using GM techniques. Results: Three shape differences were found to be possible predictors of genetic risk. These included shorter maxillary anterior teeth overall, square shaped lateral incisors on the left side, as well as lateral incisors and canines with long axes angled inward toward the midline on the left side. Both the case and control groups were found to be significantly different than the proposed ideal values of tooth proportions. Conclusions: Significant differences in anterior dental morphology were found between cases and controls, with controls displaying a more ideal dental morphology than cases for most evaluated measures. The identification of these distinct dental features in carriers of NSCL/P genetic risk factors further characterizes the phenotypic spectrum of NSCL/P which can enhance the power of genetic studies.

Although the evolutionary implications of natural differences in flow regime have been well-studied, the evolutionary implications of anthropogenic modification of flow regime are not well understood. To begin to characterize the evolutionary consequences of dam construction for small stream fishes, I conducted four related studies focused on the blacktail shiner (Cyprinella venusta). First, to characterize natural variation in body shape related to stream characteristics, I assessed the extent to which body shape corresponded to three environmental factors, phylogeny, and body size. Morphology was predominantly associated with variation in mean annual runoff; phylogeny was also a strong predictor of morphology. In contrast to previous studies that emphasize the importance of water velocity, these findings indicate that morphological variation is tightly linked to more complex aspects of hydrology and evolutionary history. Second, to initially characterize variation in body shape associated with dam construction, I compared the body shape of C. venusta from pairs of river and reservoir sites. River populations differ from reservoir populations in several aspects of morphology, demonstrating that reservoir characteristics drive changes in the morphology fish populations. Third, to characterize the pace and trajectory of phenotypic responses to impoundment, I examined a chronosequence of museum specimens originating from a reservoir and nearby stream. The rate of change in larger individuals was greatest in the first 15 years following impoundment, with less pronounced shifts in smaller individuals and no shift in the morphology of stream individuals. These results indicate that morphological responses to impoundment are decadal-scale and attenuate. Fourth, to determine if impoundment-related morphological shifts are genetically determined and are functionally significant, I conducted swimming performance trials with common garden C. venusta originating from reservoir and stream parents. Common garden juveniles exhibited differences in morphology similar to those between wild-caught individuals, indicating that morphological divergence following impoundment is an outcome of rapid evolutionary change. Offspring did not differ in swimming performance, suggesting that morphological evolution may be driven by reproductive or trophic shifts following impoundment. Together, these studies demonstrate that impoundment is a potent evolutionary force on fishes, but the mechanisms by which it does so remain relatively unknown. / acase@tulane.edu

acase@tulane.edu

<p>This thesis deals with the evolution of individuals within a species adapted to utilize specific resources, i.e. resource polymorphism. Although a well-known phenomenon, the understanding of the mechanisms behind is not complete. Considering the ruling theories, resource polymorphism is suggested to depend on severe competition for resources, the presence of open niches to be occupied leading to a reduction in competition, and disruptive selection where generalist are out-competed due trade-offs in foraging efficiency for different prey. In order to study resource polymorphism, I have used fish as the animal group in focus and the methods I have used range over laboratory experiments, field experiments, literature surveys and theoretical modelling.</p><p>In my work, I have showed that different resource use induces different body shapes and that the rate of change is dependent of the encounter rate of different resources. The induced body changes partly led to increased foraging efficiency but surprisingly I did not find any trade-offs due to specialization. However, when studying predation risk in relation to resource polymorphism, my studies point towards that resource use and predation risk may act as balancing factors in such a way that disruptive selection can take place.</p><p>My work also shows that population feedbacks have to be explored when considering the evolution of resource polymorphism. In pond and field experiments, I found that changes in resource densities affected the actual resource use despite previous adaptations to certain resources. By performing a literature survey, I found that cannibalism indirectly by its effect on population dynamics seems to facilitate the evolution of resource polymorphism. Modelling a size-structured population, I found that resource dynamics were stabilized, and the relative availability of different resources was levelled out due to cannibalism.</p><p>Taken together, my studies strongly suggest that to understand the development of resource polymorphism in consumer populations, future studies have to include the effect of a dynamic environment both with respect to resources and predators.</p>

The human bony pelvis has evolved into its current form through competing selective forces. Bipedalism and parturition of large headed babies resulted in a form that is a complex compromise. While the morphology of the human pelvis has been extensively studied, the changes that have occurred since the adoption of the modern form, the secular changes that continue to alter the size and shape of the pelvis, have not received nearly as much attention. This research aims to examine the changes that have altered the morphology of the human bony pelvic girdle of individuals in the United States born between 1840 and1981. Secular changes in the human skeleton have been documented. Improvements in nutrition, decreased disease load, exogamy, activity, climate, and other factors have led to unprecedented growth in stature and weight. The size and shape of the pelvic canal, os coxa, and bi-iliac breadth were all examined in this study. Coordinate data from males and females, blacks and whites were digitized. Calculated inter-landmark data was analyzed using traditional metric methods and the coordinate data was analyzed using 3D geometric morphometrics. After separating the samples into cohorts by sex and ancestry, results indicate that there is secular change occurring in the modern human bony pelvis. Changes in shape are significant across the groups while only white males exhibit increases in size. The dimensions of the pelvic canal have changed over time. The birth canal is becoming more rounded with the inlet anteroposterior diameter and the outlet transverse diameter becoming longer. These diameters, once limiters, are believed to have led to an adoption of the rotational birth method practiced by modern humans. In addition, the bowl of the pelvis is becoming less flared. Childhood improvements in nutrition and decreases in strenuous activity may be the cause of the dimension changes in the bony pelvis. The similar changes across both sexes and ancestries indicate a similar environmental cause. However, it is likely a combination of factors that are difficult to tease apart. Whether the increases continue remains to be determined.

This thesis deals with the evolution of individuals within a species adapted to utilize specific resources, i.e. resource polymorphism. Although a well-known phenomenon, the understanding of the mechanisms behind is not complete. Considering the ruling theories, resource polymorphism is suggested to depend on severe competition for resources, the presence of open niches to be occupied leading to a reduction in competition, and disruptive selection where generalist are out-competed due trade-offs in foraging efficiency for different prey. In order to study resource polymorphism, I have used fish as the animal group in focus and the methods I have used range over laboratory experiments, field experiments, literature surveys and theoretical modelling. In my work, I have showed that different resource use induces different body shapes and that the rate of change is dependent of the encounter rate of different resources. The induced body changes partly led to increased foraging efficiency but surprisingly I did not find any trade-offs due to specialization. However, when studying predation risk in relation to resource polymorphism, my studies point towards that resource use and predation risk may act as balancing factors in such a way that disruptive selection can take place. My work also shows that population feedbacks have to be explored when considering the evolution of resource polymorphism. In pond and field experiments, I found that changes in resource densities affected the actual resource use despite previous adaptations to certain resources. By performing a literature survey, I found that cannibalism indirectly by its effect on population dynamics seems to facilitate the evolution of resource polymorphism. Modelling a size-structured population, I found that resource dynamics were stabilized, and the relative availability of different resources was levelled out due to cannibalism. Taken together, my studies strongly suggest that to understand the development of resource polymorphism in consumer populations, future studies have to include the effect of a dynamic environment both with respect to resources and predators.

Conodonts are important biostratigraphic tools for the delineation and calibration of the geochronological boundaries within the Carboniferous and used generally as stage-boundary markers for the Lower Carboniferous (Mississippian). The studied successions in the Central and Eastern Taurides were mainly deposited in a shallow marine environment during the Carboniferous time and they comprise less abundant and diversified conodont fauna. The conodont fauna represented by twenty-four species belonging to ten genera have been described from the studied sections (AS / AAO / BSE and HB) in the Taurides (Turkey). Regarding the first occurrences and last occurrences of biostratigraphically significant species within these sections, the following zones have been established across the Lower Carboniferous successions: (1) Polygnathus inornatus Zone (Hastarian-Lower Tournaisian) / (2) Gnathodus cuneiformis &ndash / Polygnathus communis communis Zone (Ivorian-Upper Tournaisian) / (3) Polygnathus mehli mehli Zone (Ivorian-Upper Tournaisian) / (4) Gnathodus girtyi girtyi Zone (Brigantian-Upper Visean) / (5) Gnathodus girtyi simplex Zone (Pendelian-Lower Serpukhovian) / (6) Rhachistognathus muricatus Zone (Zapaltyubinsky-Upper Serpukhovian) and (7) Declinognathodus inaequalis - Declinognathodus noduliferus Zone (Bogdanovsky-Lower Bashkirian). Based on the recovered conodont assemblages, Visean - Serpukhovian boundary has been recognized by the first occurrence of Gnathodus girtyi simplex and the Mid-Carboniferous boundary is delineated by the first occurrence of Declinognathodus inaequalis, which is an index taxon for the basal part of the Bashkirian. Based on the microfacies studies it can be concluded that conodont elements essentially obtained from the crinoidal bioclastic packstone-grainstone, crinoidal packstone-grainstone, coated bioclastic grainstone, intraclastic grainstone and spiculite packstone facies in the studied sections. It can be concluded that that the occurrence of abundant crinoids are indicative criteria for the presence of conodonts. Additionally, geometric morphometric analyses of biostratigraphically important genus, Gnathodus, on the basis of data available in the literature emphasize the importance of numerical taxonomy and reveal that taxonomic revision of this genus is needed.