A dissertation submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of requirements for the degree of Master of Science. Johannesburg, 2015. / Ovahimba people from Kunene region, northern Namibia, are known for covering their bodies with red ochre mixed with clarified butterfat, traditionally known as otjize to give them a distinct red appearance. Ochre refers to a clay-like earth pigment which contains some form of iron-containing mineral. A mixture of traditional herbs with clarified butterfat, otjizumba, is also applied around the necks as a perfume. This study was prompted by ethnographic interviews amongst the Ovahimba people which revealed functional uses of the traditional cosmetics, specifically the red ochre-derived cosmetic, as a mosquito repellent. Several analytical techniques were used to determine the presence of mosquito repellent compounds in the red ochre- derived cosmetic and the aromatic plant derived-cosmetic. GC-MS was used to identify the presence of compounds which have previously been found to have mosquito repellent capabilities. GC-MS analysis identified mostly oxygenated compounds which include ketones (2-dodecanone, 2-nanonone, 2-undecanone and 2-tridecanone), aldehydes (heptanal and nonanal) and carboxylic acids (hexanoic acid and heptanoic acid) in dichloromethane extracts of otjize and mostly hydrocarbons (o-cymene, α-pinene, limonene, and squalene) and less oxygenated compounds (terpinen-4-ol and α-campholenal) in plant derived cosmetic extracts. The chemical composition of the cosmetics was also analyzed using FTIR. FTIR analysis for organics in both cosmetics showed presence of vibrational motions including O-H, C=O, C-H, C=C and C-C which affirmed the presence of organic functional groups including aldehydes, ketones, esters, alkenes and alkanes. Peak patterns observed using GC-FID showed that the mixture of red ochre and clarified butterfat released higher quantities of volatiles than when individual samples were analyzed. Mineralogical composition of red ochre was determined by PXRD, supported by FTIR which revealed as significant amount of hematite (Fe2O3), the primary mineral responsible for the red hue of the ochre. Other major minerals including quartz (SiO2), kaolinite (Al2(Si2O5)(OH)4, calcites (CaCO3) and chalconatronite(Na2Cu(CO3)2.3H2O) were found to be present in the ochre powder. Elemental analysis of the ochre determined using EDXRF and ICP-OES supported mineralogical composition as iii Ovahimba red ochre exhibited high content of iron (Fe) and silicon (Si) and a significant amount of aluminum (Al), calcium (Ca) and copper (Cu). Based on % weight, presence of transition metals in red ochre powder identified using ICP-OES was observed in the descending order; Fe> V> Cu> Au> Ti> Zr. Based on the analysis carried out in this study, it is suggested that red ochre provides catalytic role, due to its diverse metal content especially the presence of transition metals including Fe and Cu, which might be influencing the production of secondary products during autoxidation of fatty acids present in otjize, specifically ketones and aldehydes. It was also concluded that the composition of clarified butterfat could be attributed to the release of mosquito repellent compounds in the red ochre derived cosmetic because when animal fat (kudufat) was used as an organic binder, the mixture did not release any of the identified possible mosquito repellent compounds. Keywords: Aldehydes, autoxidation, clarified butterfat, fatty acids, ketones, mosquito repellents, and red ochre

Les moustiques An. coluzzii sont des vecteurs du paludisme humain en Afrique sub- saharienne (Fontenille et al., 2003). Ils s’infectent après une prise de repas de sang chez un l’hôte humain porteur de gamétocytes. Les études sur la résistance/sensibilité d’An. coluzzii au parasite P. falciparum définissent sa compétence vectorielle (Ndiath et al., 2011 ; Fryxell et al., 2012 ; Gnémé et al., 2013 ; Boissière et al., 2013). La compétence vectorielle d’An. coluzzii au parasite P. falciparum est déterminée par des gènes pro/antiparasitaires, dont TEP1 qui montre un polymorphisme à l’origine de la résistance/sensibilité des moustiques vis-à-vis de P. berghei, parasite de rongeur (Baxter et al., 2007 ; Blandin et al., 2009). Dans nos travaux nous montrons que TEP1 est un facteur antiparasitaire majeur dans la réponse contre P. falciparum, mais n’explique pas seul la résistance/sensibilité des moustiques vis-à-vis du parasite. D’autres facteurs génétiques pro/antiparasitaires non encore déterminés seraient impliqués dans la compétence vectorielle chez les moustiques An. coluzzii. Pour identifier les gènes pro/antiparasitaires impliqués dans les interactions An. coluzzii – P. falciparum, et étudier l’effet de leur polymorphisme sur la résistance/sensibilité des moustiques vis-à-vis du parasite, nous avons réalisé sur le terrain, à Mfou au Cameroun, des infections expérimentales avec des isolats naturels de P. falciparum chez les moustiques L3-5, S1low et S1high sélectionnés pour leur résistance/sensibilité à P. berghei. Les moustiques Ngousso sont utilisés ici comme contrôle de l’infectivité des parasites. / Anopheles coluzzii mosquitoes are vectors of human malaria in sub-Saharan Africa. Still, even within a vector species, the ability of mosquitoes to carry malaria parasites varies extensively between individuals, with some mosquitoes that eliminate all parasites, and are therefore unable to transmit the disease. Polymorphism in the complement-like protein TEP1 was shown to contribute to determine mosquito susceptibility to the murine malaria parasite P. berghei (Blandin et al., 2009) as well as to the human malaria parasite P. falciparum (White et al., 2010). Still, we demonstrated that TEP1 alone could not fully explain mosquito resistance and we set up to identify additional genetic factors that determine mosquito vector competence in the Ngousso line that was recently colonised in Cameroon and whose phenotype range varies extensively when exposed to P. berghei infection. To be independent from variations in the TEP1 locus, we first selected a parental line homozygous for a single TEP1 allele, TEP1*S1, that was previously linked to mosquito susceptibility. We then created isofemale families and selected them according to their phenotype upon infection with the murine malaria parasite P. berghei over several generations to create two lines carrying either many (S1high) or few (S1low) parasites. To identify the regions of the genomes that are linked to this phenotypic difference, we performed crosses and QTL mapping. To test whether the phenotypic difference selected upon P. berghei infections was conserved for P. falciparum, we subjected our two lines to blood meals infected with natural isolates of the human parasite collected in Cameroon. Results of the selection process and field infections will be presented.

In highly competitive industries where firms aren’t protected by barriers to entry and consumers’ preferences are constantly changing, the willingness and ability to adapt and continually improve operations may be critical for continued success. However, the internal exchanges between employees and managers that may be an integral part of the development and implementation of new ideas are often not modeled or included in our standard economic theories. In this research I investigate whether a higher level of employee voice (or employee input) predicts higher levels of continual improvement, and whether this continual improvement predicts higher levels of firm performance. Additionally, I will study whether humble managers keep this process going by fostering an environment where employees feel free to provide input. If this chain of events can help us better understand differences in firm performance then perhaps we can enhance our models by measuring and including these internal firm characteristics instead of simply leaving them inside the error term and calling them “unobservable.” Results across three separate studies show that humble managers are more likely to be perceived as making continual improvements and higher levels of perceived continual improvement leads to both greater levels of employee voice and fewer perceived job obstacles. A pilot study involving two separate quick-food restaurant chains also lends support for the above ideas, but uses sales in dollars as the measure for performance. Additionally, holding the employee constant in a fixed-effects analysis shows that the same employee is more likely to voice ideas to a manager he or she reports as continually improving the way things are done.

Ces dernières années la diffusion vidéo "de salon" a connu trois transitions majeures : la compression vidéo aévolué de la norme MPEG2 à la norme h.264, les écrans à tube cathodique ont disparu du marché des téléviseurs quiest actuellement dominé par les écrans à cristaux liquides (LCD) et pour nir le format haute-définition (1280x720pixels ou 1920x1080) supplante de plus en plus le format SD (576x720). Ces évolutions ont modifié l'importancedes différents types de défauts de compression pour la perception de la qualité d'une vidéo. Les défauts majeursde compression vidéo sont désormais le ou et les défauts temporels. Le terme défaut temporel regroupe ici lavariation temporelle de défauts spatiaux comme l'effet de bloc et des défauts spécifiquement temporels comme lebruit de moustique.Nous nous sommes tout d'abord focalisés sur la correction du bruit de moustique. Le correcteur que nousproposons, le TVIF, est adapté aux trois caractéristiques de ce défaut : faible amplitude par rapport au contenulocal, proximité des contours et variation temporelle. Nous avons évalué l'efficacité de notre correcteur avec desmétriques objectives mais, celles-ci ne permettant pas de conclure sur les performances de notre ltre, nousavons organisé une expérience subjective de qualité. Les données recueillies lors de cette expérience indiquentque les observateurs perçoivent notre filtre comme une amélioration et que la version spatio-temporelle de notrecorrecteur est préférée à sa version spatiale. Reboucler sur les évaluations objectives nous permet de conclure queles métriques objectives ne mesurent pas adéquatement la correction du bruit de moustique, ni l'apport de lacorrection spatio-temporelle par rapport à la correction spatiale.Nous avons ensuite organisé une expérience couplant évaluation de qualité (globale et temporelle) et enregistrementsdes positions oculaires des observateurs. Cette expérience nous permet de spécifier plusieurs pointsutiles pour réaliser une métrique objective de qualité temporelle. Par exemple, le défaut le plus gênant pour laperception de la qualité globale est la variation d'effet de bloc, qui doit donc être la priorité d'une métrique dequalité temporelle. L'analyse des mouvements oculaires des observateurs en tâche libre, tâche de qualité globaleet tâche de qualité temporelle montre, entre autres, que la qualité de la vidéo diffusée n'a pas d'influence visiblesur les endroits regardés par les participants mais influence fortement la durée des fixations. / Home video has gone through three major transitions within the past years: from the MPEG2 videocompression norm to the h.264 one, from cathode ray tube screens to liquid crystal display screens andfrom standard definition (576x720) to High-Definition (1280x720 or 1920x1080). Those changes havemodified the importance of each type of compression artifacts for quality assessment, relatively to oneanother. The two main compression artifacts are currently blur and temporal defects, including under thisterm temporal variations of spatial defects and artifacts specifically temporal such as mosquito noise.We first focused on filtering mosquito noise and presented the TVIF, a corrector adapted to this noise:small amplitude compared to the nearest edge, proximity to edges and variation through time. We firsttried to use objective quality metrics to assess the performance of our corrector. As it proved to beinconclusive, we set up a subjective experiment which showed that observers perceived our corrector asan enhancement and that they preferred the spatio-temporal correction to the spatial one. Going backover objective evaluations with the subjective ones showed that the metrics we used correctly assessneither the correction nor the gain in quality of the spatio-temporal correction over the spatial one.We then set up an experiment combining quality evaluation (both global and temporal) and recordings ofeye movements. The aim of this experiment is to specify some key points for designing an objectivetemporal quality metric. One example is that observers found that temporal variations of blocking effectare the most annoying defect global quality and, as such, should be the main focus of a temporal qualitymetric. Analysis of eye-movements of observers during free task, global quality task and temporal qualitytask shows, among other things, that quality has no visible influence on the places observers watch buthighly influences the duration of fixations. The evolutions over time of all the oculomotor parameters(saccades speed, fixation locations and durations) are similar for the three tasks during the first 1 or 2seconds of the videos and that the differences between tasks appear later on. It seems that the task toassess video quality plays a role afterwards on the deployment of visual attention.

abstract: The role of climate change, as measured in terms of changes in the climatology of geophysical variables (such as temperature and rainfall), on the global distribution and burden of vector-borne diseases (VBDs) remains a subject of considerable debate. This dissertation attempts to contribute to this debate via the use of mathematical (compartmental) modeling and statistical data analysis. In particular, the objective is to find suitable values and/or ranges of the climate variables considered (typically temperature and rainfall) for maximum vector abundance and consequently, maximum transmission intensity of the disease(s) they cause. Motivated by the fact that understanding the dynamics of disease vector is crucial to understanding the transmission and control of the VBDs they cause, a novel weather-driven deterministic model for the population biology of the mosquito is formulated and rigorously analyzed. Numerical simulations, using relevant weather and entomological data for Anopheles mosquito (the vector for malaria), show that maximum mosquito abundance occurs when temperature and rainfall values lie in the range [20-25]C and [105-115] mm, respectively. The Anopheles mosquito ecology model is extended to incorporate human dynamics. The resulting weather-driven malaria transmission model, which includes many of the key aspects of malaria (such as disease transmission by asymptomatically-infectious humans, and enhanced malaria immunity due to repeated exposure), was rigorously analyzed. The model which also incorporates the effect of diurnal temperature range (DTR) on malaria transmission dynamics shows that increasing DTR shifts the peak temperature value for malaria transmission from 29C (when DTR is 0C) to about 25C (when DTR is 15C). Finally, the malaria model is adapted and used to study the transmission dynamics of chikungunya, dengue and Zika, three diseases co-circulating in the Americas caused by the same vector (Aedes aegypti). The resulting model, which is fitted using data from Mexico, is used to assess a few hypotheses (such as those associated with the possible impact the newly-released dengue vaccine will have on Zika) and the impact of variability in climate variables on the dynamics of the three diseases. Suitable temperature and rainfall ranges for the maximum transmission intensity of the three diseases are obtained. / Dissertation/Thesis / Doctoral Dissertation Applied Mathematics 2018

This is a study of the creation and negotiation of different forms of knowledge about dengue fever. I explore how anthropology, in collaboration with ideas and practices drawn from science and art, may transform public understandings of dengue. Dengue is a vector-borne disease transmitted to humans by the bite of a mosquito which is infected with the dengue virus. Mosquito-borne diseases have normally been treated through vector control and the elimination of breeding sites. Until 1960, the use of the pesticide DDT allowed the virtual eradication of Aedes aegypti (Ae. aegypti) in many places of the world. DDT was banned in most of the world by 1970 and by 1980 the focus on vector-control was replaced by a discourse of sanitation, in which health authorities tried to ‘educate’ populations and ‘teach’ proper hygienic habits to avoid mosquito-human contact. At present, these practices are changing again. The World Health Organisation (WHO) suggests that dengue incidence could be reduced at least 50% by 2020 through applying health campaigns and social interventions that involve having people participating in the control of dengue outbreaks. In this thesis I explore how WHO guidelines are applied in the control of dengue in Medellín, and how we can think about the concepts of ‘knowledge’, ‘education’ and public health campaigns through ethnographic methods. My project has been about looking at how different understandings – or different forms of knowledge – are part of interactions of different ‘publics’, non-expert citizens, virologists, entomologists and artists. My argument is that health campaigns should be re-designed – privileging relations and stimulating debate – by focusing on experience and moving towards managing the disease and living with the mosquito. Contrary to the different models enacted in health campaigns – which neglect the value of everyday experiences – I advocate for interdisciplinary collaboration as a relational art strategy that can generate an intersubjective exchange of experiences.

The juvenile hormones (JHs) are sesquiterpenoid compounds that play a central role in insect reproduction, development and behavior. They are synthesized and secreted by a pair of small endocrine glands, the corpora allata (CA), which are intimately connected to the brain. The enzymes involved in the biosynthesis of JH are attractive targets for the control of mosquito populations. This dissertation is a comprehensive functional study of five Aedes aegypti CA enzymes, HMG-CoA synthase (AaHMGS), mevalonate kinase (AaMK), phosphomevalonate kinase (AaPMK), farnesyl diphosphate synthase (AaFPPS) and farnesyl pyrophosphate phosphatase (AaFPPase). The enzyme AaHMGS catalyzes the condensation of acetoacetyl-CoA and acetyl-CoA to produce HMG-CoA. The enzyme does not require any co-factor, although its activity is enhanced by addition of Mg2+. The enzyme AaMK is a class I mevalonate kinase that catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Activity of AaMK is inhibited by isoprenoids. The enzyme AaPMK catalyzes the cation-dependent reversible reaction of phosphomevalonate and ATP to form diphosphate mevalonate and ADP. The enzyme AaFPPS catalyzes the condensation of isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) to form geranyl diphosphate (GPP) and farnesyl pyrophosphate (FPP). The enzyme AaFPPS shows an unusual product regulation mechanism, with chain length final product of 10 or 15 C depending on the metal cofactor present. The enzymes AaFPPase-1 and AaFPPase-2 efficiently hydrolyze FPP into farnesol, although RNAi experiments demonstrate that only AaFPPase-1 is involved in the catalysis of FPP into FOL in the CA of A. aegypti. This dissertation also explored the inhibition of the activity of some of the JH biosynthesis enzymes as tools for insect control. We described the effect of N-acetyl-S-geranylgeranyl-L-cysteine as a potent inhibitor of AaFPPase 1 and AaFPPase-2. In addition, inhibitors of AaMK and AaHMGS were also investigated using purified recombinant proteins. The present study provides an important contribution to the characterization of recombinant proteins, the analysis of enzyme kinetics and inhibition constants, as well as the understanding of the importance of these five enzymes in the control of JH biosynthesis rates.

Les MVs sont un problème majeur de santé publique. L'émergence des MVs nécessite de nouveaux outils pour la surveillance de ces maladies. Ce projet s’intéresse aux deux familles de vecteurs: Ixodidae (R. sanguineus, D. reticulatus et I. ricinus) et Anophèles (An. gambiae s.l. et An. funestus). Une revue synthétise les données actuelles des MTTs et leur vectors, avant de présenter des méthodes de surveillance de ces maladies. La partie expérimentale s'est concentré sur l'élaboration de méthodes pour la sélection des utiles protéines salivaires pour l'évaluation du contact hôte-vecteur. Pour Ixodidae, la stratégie antigénique utilisée a permis d’identifier des protéines salivaires antigéniques communes et spécifiques d’espèce de ces tiques. Ces protéines pourraient servir pour l’évaluation de l’exposition de l’hôte aux Ixodidae. Pour Anophèles, la stratégie candidate utilisée a révélé une protéine salivaire antigénique d’Anopheles (f-5’nuc) pouvant être marqueur prometteur distinguant l'exposition aux Anophèles au niveau de l'espèce. Pour conforter ces résultats, l’établissement d’une relation entre la cinétique des réponses d'anticorps de l’hôte contre ces candidats salivaires, la faune Culicidienne et la variation de densité des populations de moustiques est en cours. Ce projet a souligné que tous les deux vectors peuvent induire une réponse immunitaire chez leur hôte contre des protéines salivaires antigéniques injectées. Il a permis également d’identifier des protéines salivaires permettant la discrimination de l'exposition d'hôte aux vecteurs au niveau du genre ou de l’espèce, offrant de nouvelles stratégies pour la surveillance des MVs. / Vector-borne diseases (VBD) are a major health problem worldwide. The emergence of VBD requires novel monitoring tools. The present project focused on two vector families: Ixodidae (R. sanguineus, D. reticulatus and I. ricinus) and Anopheles (An. gambiae s.l. and An. funestus). A review updates the repartition of TBD, their vectors in Europe, prior to present the different tools for monitoring of TBD transmission. The experimental part focused on establishing methods for selection of useful vector salivary proteins for host-vector contact assessment. Concerning Ixodidae, the studied antigenic strategy successfully identified the shared and discriminant tick salivary antigenic proteins. These identified proteins could be an useful tool to measure host exposition to Ixodidae bites. Concerning Anopheles, the studied candidate strategy revealed an salivary antigenic protein ( f-5’nuc) that could be a promising antigenic marker to distinguish malaria vector exposure at the species level. To comfort these results, the relationship between the kinetic host antibody response against anopheline salivary candidates and the Anopheles fauna population and density variations is under progress. The present work underlined that both two studied vector families following blood meal can elicit a host antibody response against injected vector salivary antigenic proteins. This project proposed for the first time some vector salivary proteins allowing discriminating host exposure to vector bites from genus to species level, opening new strategies for VBD monitoring at the individual and population levels.

The topic of my thesis is "Guerilla marketing and its use in commercial communications." The main objective of my thesis is to define new forms of marketing communication and to assess their possible application in the segment of small and medium-sized businesses, with particular emphasis on guerilla marketing. The thesis has three basic parts. The first two chapters present a theoretical view on marketing, commercial communications and new trends in the field, as well as on Guerilla marketing, through its definition, the definition of its main characteristics and basic types. The third chapter is formed by introducing two specific marketing campaigns using tools typical for Guerilla Marketing and was concluded with the analysis of the questionnaire.

The global incidence of dengue has increase 30-fold over the past 50 years in the western or Asian Pacific, this region is also a contemporary epicenter for resource extraction and ecological destabilization. Dengue is addition to yellow fever, chikungunya and most recently zika virus, are transmitted by the mosquito vector Aedes aegypti- a domesticated mosquito adept at breeding in artificial household containers and within homes. The history of the domestication and global distribution of Aedes aegypti is intrinsically linked to European expansion into and among tropical worlds. Contemporary population genetics research suggest the westward expansion of the mosquito vector beginning with trans-Atlantic Slave Trade moving to the Americas and then making a jump across the Pacific, which I argue occurred first within the Philippines and then spread eastward through the greater Indian Ocean. I argue that Spanish and American colonization facilitated the biological invasion of Ae. aegypti and dengue in the Philippines and created the conditions for contemporary epidemics. The discourse within the dominant voices of public health, CDC and WHO, omit this history as well as down play the significance of land use and deforestation while focusing predominantly upon dengue’s prevention and control. This omission is an act of erasure and a means of furthering western imperialism through paternalistic interventions. Mosquito-borne disease epidemics are unintended consequences of past human action and if public health discourse continues to frame epidemics as random and unfortunate events, we risk missing key patterns and continuing to perpetuate the circumstances of disease and adaptation.