Neste trabalho foi desenvolvida uma formulação para análise de sólidos bidimensionais constituídos por multiregiões utilizando-se do método dos elementos de contorno para análise linear e não linear. Para o caso de análise linear foi estudado o caso de regiões constituídas por sub-regiões de diferentes características mecânicas, utilizando-se técnicas que inicialmente consideram a compatibilidade de deslocamentos e o equilíbrio de forças na interface entre as sub-regiões, antes de se escrever as equações de equilíbrio. Inicialmente foi feita uma formulação, chamada neste trabalho de formulação singular, onde leva-se em conta apenas os deslocamentos incógnitos na interface e, posteriormente, foi desenvolvida outra formulação denominada hipersingular, onde são preservadas na interface apenas as forças de superfície. Para inclusões muito esbeltas, foi utilizada a técnica da condensação de domínios, onde o domínio 2D foi condensado inicialmente em um domínio linear de fibra e posteriormente em viga. Foi utilizada a discretização de inclusões muito esbeltas com rigidez quase nula visando a simular o comportamento de uma região de fratura elástica. A formulação foi estendida para análise não linear. A técnica das tensões iniciais foi adotada para modelar o sólido com regiões danificadas. Foi adotada a degeneração de inclusões muito esbeltas, que obedecem as leis constitutivas não lineares da mecânica do dano, simulando a origem de uma região de fratura. Para se melhorar a precisão das integrais, foi adotada a integração analítica sobre todo contorno e também sobre o domínio. Foram testados vários exemplos para validar os modelos propostos. / In this work, a boundary element formulation was developed to analyze 2D multiregions solids formed in the context of linear and non- linear analysis. Linear analysis was adopted to study problems containing regions with diferent elastic parameters. This formulation was used to study inclusion that could be degenerated to thin inclusion to represent the behavior of fibers and beams embedded in the main solid. For the linear problems, the sub-regions were adopted to represent structural elements with diferent mechanical characteristics. The sub regions were joined together by assuming the classical hypotheses of displacement compatibility and traction equilibrium along the interfaces, but applied before the approximation of the boundary and interface values. The alternative sub-region technique was developed initially to eliminate traction values along the interfaces, introducing therefore only unknown displacements. The technique was then modified to eliminate all displacements along the interface preserving the traction as unknowns. For the case of very thin inclusions the formulation has been simplified to simulate fiber and beam reinforcements. Appropriate displacement approximations across the thin sub-region have been assumed. In this inclusion was also analyzed with the elastic modulus degenerating to zero, simulating therefore a crack problem. The formulation has been extended to non-linear analysis. The initial stress procedure has been adopted to model solid with damaged regions. The damaged regions were assumed to be very small to simulate non-linear crack behavior governed by damage mechanic models. To improve the quality of the results all boundary and domains were integrated analytically. Many examples have been tested to certify that the proposed models are reliable.

Les travaux présentés dans cette thèse concernent des problématiques d'architecture logicielle multi-domaines pour le développement d’applications distribuées. Ces applications sont caractérisées aujourd’hui comme des systèmes ouverts, complexes, hétérogènes et à large échelle. Les approches traditionnelles, telles que l’approche orienté objet, n’offrent plus un paradigme de conception suffisant pour appréhender la complexité de tels systèmes. Ces nouvelles tendances ont conduit à l’émergence d’approches de plus haut niveau telles que les approches orientées services, composants ou agents. Chacune de ces approches offrent des intérêts et des caractéristiques propres dans le développement d’applications distribuées. Les services offrent une abstraction et une interopérabilité à large échelle. Abstraction dans le sens où un service permet de spécifier un élément fonctionnel sans préciser comment cet élément est implémenté. Les composants sont une approche robuste basée sur la composition et la réutilisation d’éléments clairement définis par leurs interfaces. Les agents sont eux des éléments présentant un comportement dynamique dirigé par un but et des interactions de haut niveau avec les autres agents formant l’application, vue comme une organisation de services collaboratifs. D’un point de vue conceptuel, le service peut donc être perçu comme le modèle « métier » de l’application, alors que les composants et les agents constituent un modèle d’implémentation. L’étude de ces différents domaines et des modèles associés, a montré que les approches composants et agents sont complémentaires, les points forts d’une approche représentant les faiblesses de l’autre. Face à ce constat, il nous est paru intéressant d’intégrer ces deux approches, au sein d’une même démarche de conception. Cela permet, d’une part, qu’une approche puisse bénéficier des intérêts de l’autre et d’autre part, d’utiliser conjointement des agents et des composants dans la conception d'une même application. La démarche que nous avons adoptée consiste à considérer les services comme pivot d’interaction afin de rendre possible l’interopérabilité des agents et des composants. Pour supporter cette démarche, nous avons défini un processus de conception basé sur l’Ingénierie Des Modèles qui contient quatre modèles conceptuels (Domain Specific language) dont l’intérêt est de mettre l’accent sur les concepts de services et d’interaction. Nous avons ainsi défini un modèle de services, un modèle de composants et un modèle d’agents. Enfin, un modèle mixte appelé CASOM, Component Agent Service Oriented Model, permet de spécifier une application via une combinaison des trois domaines précédents. Ensuite, des règles de correspondances ont été définies entre les quatre modèles pour pouvoir par exemple transformer une spécification agents en une spécification composants ou mixte. L'implémentation de ces transformations a été réalisée en langage ATL (ATLAS Transformation Language). / The presented work considers problems related to multi-domain software architecture for the development of distributed applications. These applications are large-scaled, heterogeneous, open and complex software systems. Traditional approaches such as object-oriented are no longer sufficient to represent such complex systems. These trends lead to the emergence of higher-level approaches such as service-oriented, components or agents. Each one of these approaches offers interests and characteristics in the development of distributed applications. Services provide an abstraction and interoperability in a large scale. Abstraction is in the sense that a service can specify a functional element without specifying how this element is implemented. The components are a robust approach based on composition and reusability through their clearly defined interfaces. Agents are elements which are characterized by dynamic goal directed behaviours and high-level interactions with other agents forming the application, seen as an organization for collaborative services. From a conceptual point of view, the service can be seen as the “business” model of an application, while components and agents are the implementation models. The study of these different domains, with their related models, showed that the components and agents approaches are complementary; the strengths of one approach overcome the weaknesses of the other. Therefore, we are interested in the integration of these two approaches in a single design approach. This allows an approach to benefit from the interests of the other, on one hand and the use of agents and components jointly in the design of an application on the other hand. To reach our objective, we consider services as pivot of interaction between agents and components. The result of our analysis leads us to develop a design process based on Model-Driven Engineering which contains four conceptual models (Domain Specific Languages) with the main interest of focusing on the concepts of services and interaction. We then defined a service, component and agent models. Finally, a hybrid model called CASOM, Component Agent Service Oriented Model, was proposed that allows application specification via a combination of the three domains. Then, mapping rules have been defined between the four models in order to transform agents specification into components specification or mixed. The implementation of these transformations was done in ATL language (ATLAS Transformation Language).

All human beings are endowed with unique talents and potentialities, and are able to bring to life the hidden gifts or the talisman within them. Being in the prime of their lives, youth should be engaged in developing themselves, acquiring virtues, and striving for excellence. Reports from schools and other sources, however, indicate that many South African youth have become prey to various social ills, such as teenage pregnancy, violence, crime, and drugs and alcohol abuse, which inhibit them from achieving their talisman. In response to this situation, a life skills programme was designed to awaken the sense of talisman in youth. Believing that all human beings are gifted with special talents and potentialities, the Youth Enrichment Programme (YEP) aims to improve and transform the cognitive, social, and moral behaviour of youth, thus bringing them closer to the state of talisman. The programme intends to equip youth with values and skills that improve their social, moral and cognitive behaviour, so that they can become responsible citizens in a united and peaceful society. It pays special attention to the moral and spiritual aspects of the learners, aspects severely neglected by most education systems. The principles of YEP are founded on the spiritual nature of man, the oneness of mankind, unity in diversity, and equality. The programme adopts transformational and multi-domain teaching strategies to transform learners through interactive critical assessment of values in the context of real-life moral and social issues. The main purpose of this study was to analyse and evaluate YEP in terms of its influence on the cognitive, social, and moral behaviour of adolescent learners and to determine its strengths and weaknesses. Various aspects of the programme were investigated, including its underlying theoretical principles, methodological approaches and design characteristics. The researcher evaluated the influence of the programme on the behaviour of adolescent learners in five institutions in two provinces. The qualitative and quantitative data from the learners, teachers and facilitators in the five case studies showed that the programme had a positive influence on the learners in the cognitive, social and moral domains. It broadened their view of life and developed problem solving skills in the cognitive domain, enhanced their communication skills and co-operation in social domain, and improved their general moral behaviour especially in the areas of trust and respect in the moral domain. Overall, the effect of the programme was most prominent in transforming the moral behaviour of the learners, bringing some of them closer to their state of talisman. The study also discovered three unexpected phenomena - the beneficial role of peer learning facilitation, the positive effect of facilitation on the young facilitators, and the salutary effect of the programme on the general learning atmosphere in the schools. All human beings are endowed with unique talents and potentialities, and are able to bring to life the hidden gifts or the talisman within them. Being in the prime of their lives, youth should be engaged in developing themselves, acquiring virtues, and striving for excellence. Reports from schools and other sources, however, indicate that many South African youth have become prey to various social ills, such as teenage pregnancy, violence, crime, and drugs and alcohol abuse, which inhibit them from achieving their talisman. In response to this situation, a life skills programme was designed to awaken the sense of talisman in youth. Believing that all human beings are gifted with special talents and potentialities, the Youth Enrichment Programme (YEP) aims to improve and transform the cognitive, social, and moral behaviour of youth, thus bringing them closer to the state of talisman. The programme intends to equip youth with values and skills that improve their social, moral and cognitive behaviour, so that they can become responsible citizens in a united and peaceful society. It pays special attention to the moral and spiritual aspects of the learners, aspects severely neglected by most education systems. The principles of YEP are founded on the spiritual nature of man, the oneness of mankind, unity in diversity, and equality. The programme adopts transformational and multi-domain teaching strategies to transform learners through interactive critical assessment of values in the context of real-life moral and social issues. The main purpose of this study was to analyse and evaluate YEP in terms of its influence on the cognitive, social, and moral behaviour of adolescent learners and to determine its strengths and weaknesses. Various aspects of the programme were investigated, including its underlying theoretical principles, methodological approaches and design characteristics. The researcher evaluated the influence of the programme on the behaviour of adolescent learners in five institutions in two provinces. The qualitative and quantitative data from the learners, teachers and facilitators in the five case studies showed that the programme had a positive influence on the learners in the cognitive, social and moral domains. It broadened their view of life and developed problem solving skills in the cognitive domain, enhanced their communication skills and co-operation in social domain, and improved their general moral behaviour especially in the areas of trust and respect in the moral domain. Overall, the effect of the programme was most prominent in transforming the moral behaviour of the learners, bringing some of them closer to their state of talisman. The study also discovered three unexpected phenomena - the beneficial role of peer learning facilitation, the positive effect of facilitation on the young facilitators, and the salutary effect of the programme on the general learning atmosphere in the schools. / Dissertation (PhD (Curriculum Studies))--University of Pretoria, 2004. / Curriculum Studies / unrestricted

L’évolution récente des commutateurs de sélection de longueurs d’onde (WSS -Wavelength Selective Switch) favorise le développement du multiplexeur optique d’insertionextraction reconfigurable (ROADM - Reconfigurable Optical Add/Drop Multiplexers) à plusieurs degrés sans orientation ni coloration, considéré comme un équipement fort prometteur pour les réseaux maillés du futur relativement au multiplexage en longueur d’onde (WDM -Wavelength Division Multiplexing ). Cependant, leur propriété de commutation asymétrique complique la question de l’acheminement et de l’attribution des longueur d’ondes (RWA - Routing andWavelength Assignment). Or la plupart des algorithmes de RWA existants ne tiennent pas compte de cette propriété d’asymétrie. L’interruption des services causée par des défauts d’équipements sur les chemins optiques (résultat provenant de la résolution du problème RWA) a pour conséquence la perte d’une grande quantité de données. Les recherches deviennent ainsi incontournables afin d’assurer la survie fonctionnelle des réseaux optiques, à savoir, le maintien des services, en particulier en cas de pannes d’équipement. La plupart des publications antérieures portaient particulièrement sur l’utilisation d’un système de protection permettant de garantir le reroutage du trafic en cas d’un défaut d’un lien. Cependant, la conception de la protection contre le défaut d’un lien ne s’avère pas toujours suffisante en termes de survie des réseaux WDM à partir de nombreux cas des autres types de pannes devenant courant de nos jours, tels que les bris d’équipements, les pannes de deux ou trois liens, etc. En outre, il y a des défis considérables pour protéger les grands réseaux optiques multidomaines composés de réseaux associés à un domaine simple, interconnectés par des liens interdomaines, où les détails topologiques internes d’un domaine ne sont généralement pas partagés à l’extérieur. La présente thèse a pour objectif de proposer des modèles d’optimisation de grande taille et des solutions aux problèmes mentionnés ci-dessus. Ces modèles-ci permettent de générer des solutions optimales ou quasi-optimales avec des écarts d’optimalité mathématiquement prouvée. Pour ce faire, nous avons recours à la technique de génération de colonnes afin de résoudre les problèmes inhérents à la programmation linéaire de grande envergure. Concernant la question de l’approvisionnement dans les réseaux optiques, nous proposons un nouveau modèle de programmation linéaire en nombres entiers (ILP - Integer Linear Programming) au problème RWA afin de maximiser le nombre de requêtes acceptées (GoS - Grade of Service). Le modèle résultant constitue celui de l’optimisation d’un ILP de grande taille, ce qui permet d’obtenir la solution exacte des instances RWA assez grandes, en supposant que tous les noeuds soient asymétriques et accompagnés d’une matrice de connectivité de commutation donnée. Ensuite, nous modifions le modèle et proposons une solution au problème RWA afin de trouver la meilleure matrice de commutation pour un nombre donné de ports et de connexions de commutation, tout en satisfaisant/maximisant la qualité d’écoulement du trafic GoS. Relativement à la protection des réseaux d’un domaine simple, nous proposons des solutions favorisant la protection contre les pannes multiples. En effet, nous développons la protection d’un réseau d’un domaine simple contre des pannes multiples, en utilisant les p-cycles de protection avec un chemin indépendant des pannes (FIPP - Failure Independent Path Protecting) et de la protection avec un chemin dépendant des pannes (FDPP - Failure Dependent Path-Protecting). Nous proposons ensuite une nouvelle formulation en termes de modèles de flots pour les p-cycles FDPP soumis à des pannes multiples. Le nouveau modèle soulève un problème de taille, qui a un nombre exponentiel de contraintes en raison de certaines contraintes d’élimination de sous-tour. Par conséquent, afin de résoudre efficacement ce problème, on examine : (i) une décomposition hiérarchique du problème auxiliaire dans le modèle de décomposition, (ii) des heuristiques pour gérer efficacement le grand nombre de contraintes. À propos de la protection dans les réseaux multidomaines, nous proposons des systèmes de protection contre les pannes d’un lien. Tout d’abord, un modèle d’optimisation est proposé pour un système de protection centralisée, en supposant que la gestion du réseau soit au courant de tous les détails des topologies physiques des domaines. Nous proposons ensuite un modèle distribué de l’optimisation de la protection dans les réseaux optiques multidomaines, une formulation beaucoup plus réaliste car elle est basée sur l’hypothèse d’une gestion de réseau distribué. Ensuite, nous ajoutons une bande pasiv sante partagée afin de réduire le coût de la protection. Plus précisément, la bande passante de chaque lien intra-domaine est partagée entre les p-cycles FIPP et les p-cycles dans une première étude, puis entre les chemins pour lien/chemin de protection dans une deuxième étude. Enfin, nous recommandons des stratégies parallèles aux solutions de grands réseaux optiques multidomaines. Les résultats de l’étude permettent d’élaborer une conception efficace d’un système de protection pour un très large réseau multidomaine (45 domaines), le plus large examiné dans la littérature, avec un système à la fois centralisé et distribué. / Recent developments in the wavelength selective switch (WSS) technology enable multi-degree reconfigurable optical add/drop multiplexers (ROADM) architectures with colorless and directionless switching, which is regarded as a very promising enabler for future reconfigurable wavelength division multiplexing (WDM) mesh networks. However, its asymmetric switching property complicates the optimal routing and wavelength assignment (RWA) problem, which is NP-hard. Most of the existing RWA algorithms do not consider such property. Disruption of services through equipment failures on the lightpaths (output of RWA problem) is consequential as it involves the lost of large amounts of data. Therefore, substantial research efforts are needed to ensure the functional survivability of optical networks, i.e., the continuation of services even when equipment failures occur. Most previous publications have focused on using a protection scheme to guarantee the traffic connections in the event of single link failures. However, protection design against single link failures turns out not to be always sufficient to keep the WDM networks away from many downtime cases as other kinds of failures, such as node failures, dual link failures, triple link failures, etc., become common nowadays. Furthermore, there are challenges to protect large multi-domain optical networks which are composed of several singledomain networks, interconnected by inter-domain links, where the internal topological details of a domain are usually not shared externally. The objective of this thesis is to propose scalable models and solution methods for the above problems. The models enable to approach large problem instances while producing optimal or near optimal solutions with mathematically proven optimality gaps. For this, we rely on the column generation technique which is suitable to solve large scale linear programming problems. For the provisioning problem in optical networks, we propose a new ILP (Integer Linear Programming) model for RWA problem with the objective of maximizing the Grade of Service (GoS). The resulting model is a large scale optimization ILP model, which allows the exact solution of quite large RWA instances, assuming all nodes are asymmetric and with a given switching connectivity matrix. Next, we modify the model and propose a solution for the RWA problem with the objective of finding the best switching connectivity matrix for a given number of ports and a given number of switching connections, while satisfying/maximizing the GoS. For protection in single domain networks, we propose solutions for the protection against multiple failures. Indeed, we extent the protection of a single domain network against multiple failures, using FIPP and FDPP p-cycles. We propose a new generic flow formulation for FDPP p-cycles subject to multiple failures. Our new model ends up with a complex pricing problem, which has an exponential number of constraints due to some subtour elimination constraints. Consequently, in order to efficiently solve the pricing problem, we consider: (i) a hierarchical decomposition of the original pricing problem; (ii) heuristics in order to go around the large number of constraints in the pricing problem. For protection in multi-domain networks, we propose protection schemes against single link failures. Firstly, we propose an optimization model for a centralized protection scheme, assuming that the network management is aware of all the details of the physical topologies of the domains. We then propose a distributed optimization model for protection in multi-domain optical networks, a much more realistic formulation as it is based on the assumption of a distributed network management. Then, we add bandwidth sharing in order to reduce the cost of protection. Bandwidth of each intra-domain link is shared among FIPP p-cycles and p-cycles in a first study, and then among paths for link/path protection in a second study. Finally, we propose parallel strategies in order to obtain solutions for very large multi-domain optical networks. The result of this last study allows the efficent design of a protection scheme for a very large multi-domain network (45 domains), the largest one by far considered in the literature, both with a centralized and distributed scheme.

L'Internet du futur aura a transporter les donnees de nouvelles applications avec des garanties de qualite de service (QdS). De ce besoin resulte la necessite d'en re-concevoir l'architecture. Par ailleurs, la structure de l'Internet, compose de domaines independants vis a vis de la gestion de la QdS, pose le probleme de la continuite du service lors de la traversee de plusieurs domaines. Face a ces deux problematiques, la these soutenue est celle d'un systeme de communication offrant des garanties de QdS par flux applicatif dans un environnement Internet multi domaines. Son architecture integre un plan communication comportant plusieurs services/protocoles aux niveaux Transport et IP, et un plan signalisation assurant la gestion des ressources a la frontiere des domaines. Nos contributions sont les suivantes. Nous proposons un modele de caracterisation des services IP et Transport, etaye par : (1) des mesures realisees sur une plate forme nationale, (2) une etude en simulation (ns-2) et (3) des mesures realisees sur une plate-forme emulant (Dummynet) un Internet multi domaines. Nous etendons l'architecture de communication proposee dans des travaux anterieurs de facon a abstraire le niveau applicatif de la complexite du choix des services Transport et IP, et a optimiser l'utilisation des ressources du reseau. Nous specifions en UML et implementons en Java notre proposition d'architecture de signalisation permettant d'assurer la continuite du service offert aux applications sur tous les domaines traverses. Enfin, nous testons le systeme de communication avec deux types d'applications multimedias sur une plate-forme emulant le comportement de plusieurs domaines DiffServ.

Proteins are essential for the growth, survival and maintenance of the cell. Understanding the functional roles of proteins helps to decipher the working of macromolecular assemblies and cellular machinery of living organisms. A thorough investigation of the link between sequence, structure and function of proteins, helps in building a comprehensive understanding of the complex biological systems. Proteins have been observed to be composed of single and multiple domains. Analysis of proteins encoded in diverse genomes shows the ubiquitous nature of multi-domain proteins. Though the majority of eukaryotic proteins are multi-domain in nature, 3-D structures of only a small proportion of multi-domain proteins are known due to difficulties in crystallizing such proteins. While functions of individual domains are generally extensively studied, the complex interplay of functions of domains is not well understood for most multi-domain proteins. Paucity of structural and functional data, affects our understanding of the evolution of structure and function of multi-domain proteins. The broad objective of this thesis is to achieve an enhanced understanding of structure and function of protein domains by computational analysis of sequence and structural data. Special attention is paid in the first few chapters of this thesis on the multi-domain proteins. Classification of multi-domain proteins by implementation of an alignment-free sequence comparison method has been achieved in Chapters 2 and 3. Studies on organization, interactions and interdependence of domain-domain interactions in multi-domain proteins with respect to sequential separation between domains and N to C-terminal domain order have been described in Chapters 4 and 5. The functional and structural repertoire of organisms can be comprehensively studied and compared using functional and structural domain annotations. Chapter 6, 7 and 8 represent the proteome-wide structure and function comparisons of various pathogenic and non-pathogenic microorganisms. These comparisons help in identifying proteins implicated in virulence of the pathogen and thus predict putative targets for disease treatment and prevention. Chapter 1 forms an introduction to the main subject area of this thesis. Starting with describing protein structure and function, details of the four levels of hierarchical organization of protein structure have been provided, along with the databases that document protein sequences and structures. Classification of protein domains considered as the realm of function, structure and evolution has been described. The usefulness of classification of proteins at the domain level has been highlighted in terms of providing an enhanced understanding of protein structure and function and also their evolutionary relatedness. The details of structure, function and evolution of multi-domain proteins have also been outlined in chapter 1. ! Chapter 2 aims to achieve a biologically meaningful classification scheme for multi-domain protein sequences. The overall function of a multi-domain protein is determined by the functional and structural interplay of its constituent domains. Traditional sequence-based methods utilize only the domain-level information to classify proteins. This does not take into account the contributions of accessory domains and linker regions towards the overall function of a multi-domain protein. An alignment-free protein sequence comparison tool, CLAP (CLAssification of Proteins) previously developed in this laboratory, was assessed and improved when the author joined the group. CLAP was developed especially to handle multi-domain protein sequences without a requirement of defining domain boundaries and sequential order of domains (domain architecture). ! The working principle of CLAP involves comparison of all against all windows of 5-residue sequence patterns between two protein sequences. The sequences compared could be full-length comprising of all the domains in the two proteins. This compilation of comparison is represented as the Local Matching Scores (LMS) between protein sequences (nslab.iisc.ernet.in/clap/). It has been previously shown that the execution time of CLAP is ~7 times faster than other protein sequence comparison methods that employ alignment of sequences. In Chapter 2, CLAP-based classification has been carried out on two test datasets of proteins containing (i) Tyrosine phosphatase domain family and (ii) SH3-domain family. The former dataset comprises both single and multi-domain proteins that sometimes consist of domain repeats of the tyrosine phosphatase domain. The latter dataset consists only of multi-domain proteins with one copy of the SH3-domain. At the domain-level CLAP-based classification scheme resulted in a clustering similar to that obtained from an alignment-based method, ClustalW. CLAP-based clusters obtained for full-length datasets were shown to comprise of proteins with similar functions and domain architectures. Hence, a protein classification scheme is shown to work efficiently that is independent of domain definitions and requires only the full-length amino acid sequences as input.! Chapter 3 explores the limitations of CLAP in large-scale protein sequence comparisons. The potential advantages of full-length protein sequence classification, combined with the availability of the alignment-free sequence comparison tool, CLAP, motivated the conceptualization of full-length sequence classification of the entire protein repertoire. Before undertaking this mammoth task, working of CLAP was tested for a large dataset of 239,461 protein sequences. Chapter 3 discusses the technical details of computation, storage and retrieval of CLAP scores for a large dataset in a feasible timeframe. CLAP scores were examined for protein pairs of same domain architecture and ~22% of these showed 0 CLAP similarity scores. This led to investigation of the sensitivity of CLAP with respect to sequence divergence. Several test datasets of proteins belonging to the same SCOP fold were constructed and CLAP-based classification of these proteins was examined at inter and intra-SCOP family level. CLAP was successful in efficiently clustering evolutionary related proteins (defined as proteins within the same SCOP superfamily) if their sequence identity >35%. At lower sequence identities, CLAP fails to recognize any evolutionary relatedness. Another test dataset consisting of two-domain proteins with domain order swapped was constructed. Domain order swap refers to domain architectures of type AB and BA, consisting of domains A and B. A condition that the sequence identities of homologous domains were greater than 35% was imposed. CLAP could effectively cluster together proteins of the same domain architectures in this case. Thus, the sequence identity threshold of 35% at the domain-level improves the accuracy of CLAP. The analysis also showed that for highly divergent sequences, the expectation of 5-residue pattern match was likely a stringent criterion. Thus, a modification in the 5-residue identical pattern match criterion, by considering even similar residue and gaps within matched patterns may be required to effectuate CLAP-based clustering of remotely related protein sequences. Thus, this study highlights the limitations of CLAP with respect to large-scale analysis and its sensitivity to sequence divergence. ! Chapters 4 and 5 discuss the computational analysis of inter-domain interactions with respect to sequential distance and domain order. Knowledge of domain composition and 3-D structures of individual domains in a multi-domain protein may not be sufficient to predict the tertiary structure of the multi-domain protein. Substantial information about the nature of domain-domain interfaces helps in prediction of the tertiary as well as the quaternary structure of a protein. Therefore, chapter 4 explores the possible relationship between the sequential distance separating two domains in a multi-domain protein and the extent of their interaction. With increasing sequential separation between any two domains, the extent of inter-domain interactions showed a gradual decrease. The trend was more apparent when sequential separation between domains is measured in terms of number of intervening domains. Irrespective of the linker length, extensive interactions were seen more often between contiguous domains than between non-contiguous domains. Contiguous domains show a broader interface area and lower proportion of non-interacting domains (interface area: 0 Å2 to - 4400 Å2, 2.3% non-interacting domains) than non-contiguous domains (interface area: 0 Å2 to - 2000 Å2, 34.7% non-interacting domains). Additionally, as inter-protein interactions are mediated through constituent domains, rules of protein-protein interactions were applied to domain-domain interactions. Tight binding between domains is denoted as putative permanent domain-domain interactions and domains that may dissociate and associate with relatively weak interactions to regulate functional activity are denoted as putative transient domain-domain interactions. An interface area threshold of 600 Å2 was utilized as a binary classifier to distinguish between putative permanent and putative transient domain-domain interactions. Therefore, the state of interaction of a domain pair is defined as either putative permanent or putative transient interaction. Contiguous domains showed a predominance of putative permanent nature of inter-domain interface, whereas non-contiguous domains showed a prevalence of putative transient interfaces. The state of interaction of various SCOP superfamily pairs was studied across different proteins in the dataset. SCOP superfamily pairs mostly showed a conserved state of interaction, i.e. either putative permanent or putative transient in all their occurrences across different proteins. Thus, it is noted that contiguous domains interact extensively more often than non-contiguous domains and specific superfamily pairs tend to interact in a conserved manner. In conclusion, a combination of interface area and other inter-domain properties along with experimental validation will help strengthen the binary classification scheme of putative permanent and transient domain-domain interactions.! Chapter 5 provides structural analysis of domain pairs occurring in different sequential domain orders in mutli-domain proteins. The function and regulation of a multi-domain protein is predominantly determined by the domain-domain interactions. These in turn are influenced by the sequential order of domains in a protein. With domains defined using evolutionary and structural relatedness (SCOP superfamily), their conservation of structure and function was studied across domain order reversal. A domain order reversal indicates different sequential orders of the concerned domains, which may be identified in proteins of same or different domain compositions. Domain order reversals of domains A and B can be indicated in protein pair consisting of the domain architectures xAxBx and xBxAx, where x indicates 0 or more domains. A total of 161 pairs of domain order reversals were identified in 77 pairs of PDB entries. For most of the comparisons between proteins with different domain composition and architecture, large differences in the relative spatial orientation of domains were observed. Although preservation of state of interaction was observed for ~75% of the comparisons, none of the inter-domain interfaces of domains in different order displayed high interface similarity. These domain order reversals in multi-domain proteins are contributed by a limited number of 15 SCOP superfamilies. Majority of the superfamilies undergoing order reversal either function as transporters or regulatory domains and very few are enzymes. A higher proportion of domain order reversals were observed in domains separated by 0 or 1 domains than those separated by more than 1 domain. A thorough analysis of various structural features of domains undergoing order reversal indicates that only one order of domains is strongly preferred over all possible orders. This may be due to either evolutionary selection of one of the orders and its conservation throughout generations, or the fact that domain order reversals rarely conserve the interface between the domains. Further studies (Chapters 6 to 8) utilize the available computational techniques for structural and functional annotation of proteins encoded in a few bacterial genomes. Based on these annotations, proteome-wide structure and function comparisons were performed between two sets of pathogenic and non-pathogenic bacteria. The first study compares the pathogenic Mycobacterium tuberculosis to the closely related organism Mycobacterium smegmatis which is non-pathogenic. The second study primarily identified biologically feasible host-pathogen interactions between the human host and the pathogen Leptospira interrogans and also compared leptospiral-host interactions of the pathogenic Leptospira interrogans and of the saprophytic Leptospira biflexa with the human host. Chapter 6 describes the function and structure annotation of proteins encoded in the genome of M. smegmatis MC2-155. M. smegmatis is a widely used model organism for understanding the pathophysiology of M. tuberculosis, the primary causative agent of tuberculosis in humans. M. smegmatis and M. tuberculosis species of the mycobacterial genus share several features like a similar cell-wall architecture, the ability to oxidise carbon monoxide aerobically and share a huge number of homologues. These features render M. smegmatis particularly useful in identifying critical cellular pathways of M. tuberculosis to inhibit its growth in the human host. In spite of the similarities between M. smegmatis and M. tuberculosis, there are stark differences between the two due to their diverse niche and lifestyle. While there are innumerable studies reporting the structure, function and interaction properties of M. tuberculosis proteins, there is a lack of high quality annotation of M. smegmatis proteins. This makes the understanding of the biology of M. smegmatis extremely important for investigating its competence as a good model organism for M. tuberculosis. With the implementation of available sequence and structural profile-based search procedures, functional and structural characterization could be achieved for ~92% of the M. smegmatis proteome. Structural and functional domain definitions were obtained for a total of 5695 of 6717 proteins in M. smegmatis. Residue coverage >70% was achieved for 4567 proteins, which constitute ~68% of the proteome. Domain unassigned regions more than 30 residues were assessed for their potential to be associated to a domain. For 1022 proteins with no recognizable domains, putative structural and functional information was inferred for 328 proteins by the use of distance relationship detection and fold recognition methods. Although 916 sequences of 1022 proteins with no recognizable domains were found to be specific to M. smegmatis species, 98 of these are specific to its MC2-155 strain. Of the 1828 M. smegmatis proteins classified as conserved hypothetical proteins, 1038 proteins were successfully characterized. A total of 33 Domains of Unknown Function (DUFs) occurring in M. smegmatis could be associated to structural domains. A high representation of the tetR and GntR family of transcription regulators was noted in the functional repertoire of M. smegmatis proteome. As M. smegmatis is a soil-dwelling bacterium, transcriptional regulators are crucial for helping it to adapt and survive the environmental stress. Similarly, the ABC transporter and MFS domain families are highly represented in the M. smegmatis proteome. These are important in enabling the bacteria to uptake carbohydrate from diverse environmental sources. A lower number of virulent proteins were identified in M. smegmatis, which justifies its non-pathogenicity. Thus, a detailed functional and structural annotation of the M. smegmatis proteome was achieved in Chapter 6. Chapter 7 delineates the similarities and difference in the structure and function of proteins encoded in the genomes of the pathogenic M. tuberculosis and the non-pathogenic M. smegmatis. The protocol employed in Chapter 6 to achieve the proteome-wide structure and function annotation of M. smegmatis was also applied to M. tuberculosis proteome in Chapter 7. The number of proteins encoded by the genome of M. smegmatis strain MC2-155 (6717 proteins) is comparatively higher than that in M. tuberculosis strain H37Rv (4018 proteins). A total of 2720 high confidence orthologues sharing ≥30% sequence identity were identified in M. tuberculosis with respect to M. smegmatis. Based on the orthologue information, specific functional clusters, essential proteins, metabolic pathways, transporters and toxin-antitoxin systems of M. tuberculosis were inspected for conservation in M. smegmatis. Among the several categories analysed, 53 metabolic pathways, 44 membrane transporter proteins belonging to secondary transporters and ATP-dependent transporter classes, 73 toxin-antitoxin systems, 23 M. tuberculosis-specific targets, 10 broad-spectrum targets and 34 targets implicated in persistence of M. tuberculosis could not detect any orthologues in M. smegmatis. Several of the MFS superfamily transporters act as drug efflux pumps and are hence associated with drug resistance in M. tuberculosis. The relative abundances of MFS and ABC superfamily transporters are higher in M. smegmatis than in M. tuberculosis. As these transporters are involved in carbohydrate uptake, their higher representation in M. smegmatis than in M. tuberculosis highlights the lack of proficiency of M. tuberculosis to assimilate diverse carbon sources. In the case of porins, MspA-like and OmpA-like porins are selectively present in either M. smegmatis or M. tuberculosis. These differences help to elucidate protein clusters for which M. smegmatis may not be the best model organism to study M. tuberculosis proteins.! At the domain-level, ATP-binding domain of ABC transporters, tetracycline transcriptional regulator (tetR) domain family, major facilitator superfamily (MFS) domain family, AMP-binding domain family and enoyl-CoA hydrolase domain family are highly represented in both M. smegmatis and M. tuberculosis proteomes. These domains play an essential role in the carbohydrate uptake systems and drug-efflux pumps among other diverse functions in mycobacteria. There are several differentially represented domain families in M. tuberculosis and M. smegmatis. For example, the pentapeptide-repeat domain, PE, PPE and PIN domains although abundantly present in M. tuberculosis, are very rare in M. smegmatis. Therefore, such uniquely or differentially represented functional and structural domains in M. tuberculosis as compared to M. smegmatis may be linked to pathogenicity or adaptation of M. tuberculosis in the host. Hence, major differences between M. tuberculosis and M. smegmatis were identified, not only in terms of domain populations but also in terms of domain combinations. Thus, Chapter 7 highlights the similarities and differences between M. smegmatis and M. tuberculosis proteomes in terms of structure and function. These differences provide an understanding of selective utilization of M. smegmatis as a model organism to study M. tuberculosis. ! In Chapter 8, computational tools have been employed to predict biologically feasible host-pathogen interactions between the human host and the pathogenic, Leptospira interrogans. Sensitive profile-based search procedures were used to specifically identify practical drug targets in the genome of Leptospira interrogans, the causative agent of the globally widespread zoonotic disease, Leptospirosis. Traditionally, the genus Leptospira is classified into two species complex- the pathogenic L. interrogans and the non-pathogenic saprophyte L. biflexa. The pathogen gains entry into the human host through direct or indirect contact with fluids of infected animals. Several ambiguities exist in the understanding of L. interrogans pathogenesis. An integration of multiple computational approaches guided by experimentally derived protein-protein interactions, was utilized for recognition of host-pathogen protein-protein interactions. The initial step involved the identification of similarities of host and L. interrogans proteins with crystal structures of experimentally known transient protein-protein complexes. Further, conservation of interfacial nature was used to obtain high confidence predictions for putative host-pathogen protein-protein interactions. These predictions were subjected to further selection based on subcellular localization of proteins of the human host and L. interrogans, and tissue-specific expression profiles of the host proteins. A total of 49 protein-protein interactions mediated by 24 L. interrogans proteins and 17 host proteins were identified and these may be subjected to further experimental investigations to assess their in vivo relevance. The functional relevance of similarities and differences between the pathogenic and non-pathogenic leptospires in terms of interactions with the host has also been explored. For this, protein-protein interactions across human host and the non-pathogenic saprophyte L. biflexa were also predicted. Nearly 39 leptospiral-host interactions were recognized to be similar across both the pathogen and saprophyte in the context of processes that influence the host. The overlapping leptospiral-host interactions of L. interrogans and L. biflexa proteins with the human host proteins are primarily associated with establishment of its entry into the human host. These include adhesion of the leptospiral proteins to host cells, survival in host environment such as iron acquisition and binding to components of extracellular matrix and plasma. The disjoint sets of leptospiral-host interactions are species-specific interactions, more importantly indicative of the establishment of infection by L. interrogans in the human host and immune clearance of L. biflexa by the human host. With respect to L. interrogans, these specific interactions include interference with blood coagulation cascade and dissemination to target organs by means of disruption of cell junction assembly. On the other hand, species-specific interactions of L. biflexa proteins include those with components of host immune system. ! In spite of the limited availability of experimental evidence, these help in identifying functionally relevant interactions between host and pathogen by integrating multiple lines of evidence. Thus, inferences from computational prediction of host-pathogen interactions act as guidelines for experimental studies investigating the in vivo relevance of these predicted protein-protein interactions. This will further help in developing effective measures for treatment and disease prevention. In summary, Chapters 2 and 3 describe the implementation, advantages and limitations of the alignment-free full-length sequence comparison method, CLAP. Chapter 4 and 5 are dedicated to understand the domain-domain interactions in multi-domain protein sequences and structures. In Chapters 6, 7 and 8 the computational analyses of the mycobacterial species and leptospiral species helped in an enhanced understanding of the functional repertoire of these bacteria. These studies were undertaken by utilizing the biological sequence data available in public databases and implementation of powerful homology-detection techniques. The supplemental data associated with the chapters is provided in a compact disc attached with this thesis.!

L’étude de la fiabilité des systèmes mécatroniques est un axe de recherche relativement récent. Ces études doivent être menées au plus tôt au cours de la phase de conception, afin de prévoir, modéliser et concevoir des systèmes fiables, disponibles et sûrs et de réduire les coûts et le nombre de prototypes nécessaires à la validation d’un système. Après avoir défini les systèmes mécatroniques et les notions de sûreté de fonctionnement et de fiabilité, nous présentons un aperçu des approches existantes (quantitatives et qualitatives) pour la modélisation et l’évaluation de la fiabilité, et nous mettons en évidence les points d’amélioration et les pistes à développer par la suite.Les principales difficultés dans les études de fiabilité des systèmes mécatroniques sont la combinaison multi-domaines (mécanique, électronique,informatique) et les différents aspects fonctionnels et dysfonctionnels (hybride, dynamique, reconfigurable et interactif). Il devient nécessaire d’utiliser de nouvelles approches pour l’estimation de la fiabilité.Nous proposons une méthodologie d’évaluation de la fiabilité prévisionnelle en phase de conception d’un système mécatronique, en prenant en compte les interactions multi-domaines entre les composants, à l’aide de la modélisation par Réseaux de Pétri,Réseaux bayésiens et fonctions de croyance.L’évaluation de la fiabilité en phase de développement doit être robuste, avec une confiance suffisante et prendre en compte tant les incertitudes épistémiques concernant les variables aléatoires d’entrée du modèle utilisé que l’incertitude sur le modèle pris en hypothèse. L’approche proposée a été appliquée à l’«actionneur intelligent» de la société Pack’ Aero. / Reliability analysis of mechatronic systems is one of the most dynamic fields of research. This analysis must be conducted during the design phase, in order to model and to design safe and reliable systems. After presenting some concepts of mechatronic systems and of dependability and reliability, we present an overview of existing approaches (quantitatives and qualitatives) for the reliability assessment and we highlight the perspectives to develop. The criticality of mechatronic systems is due, on one hand, to multi-domain combination (mechanical, electronic, software), and, on the other hand, to their different functional and dysfunctional aspects (hybrid, dynamic, reconfigurable and interactive). Therefore, new approaches for dependability assessment should be developped. We propose a methodology for reliability assessment in the design phase of a mechatronic system, by taking into account multi-domain interactions and by using modeling tools such as Petri Nets and Dynamic Bayesian Networks. Our approach also takes into account epistemic uncertainties (uncertainties of model and of parameters) by using an evidential network adapted to our model. Our methodology was applied to the reliability assessment of an "intelligent actuator" from Pack’Aero

La tendance à la numérisation de nombreux secteurs industriels tels que l’automobile, l’agriculture, les transports, la gestion urbaine, etc. révèle la nécessité de nouveaux usages des services de communication point-à-multipoint, tels que la fourniture massive de mises à jour logicielles et livraison fiable de messages d’alerte à la population, etc. D’un autre côté, la mise en logiciel des réseaux de nouvelle génération, avec notamment l’adoption croissante des réseaux définis par logiciel (SDN), apporte au réseau la flexibilité et les capacités de programmation permettant de prendre en charge des services de distribution point-à-multipoint de manière rentable. Cette thèse contribue au problème général de la fourniture de services de communication point-à-multipoint avec des exigences de qualité de service (QoS) dans un réseau SDN multi-domaines. Il considère également que certains des domaines sont des réseaux multi-sauts sans-fil. Tout d’abord, une technique d’agrégation de topologie de domaine basée sur une arborescence de Steiner, combinée à un algorithme heuristique d’allocation de ressources, sont proposés pour prendre en charge des services point-à-multipoint couvrant plusieurs domaines. Ensuite, un service de découverte de topologie générique est proposé pour les réseaux multi-sauts sans-fil basés sur le SDN afin de permettre au contrôleur SDN de créer et de gérer une vue complète du réseau avec divers attributs de nœud et de liaison sans-fil. Le contrôleur peut alors exposer des vues personnalisées aux applications de contrôle du réseau, telles que, par exemple, l’application en charge de la fourniture de services point-à-multipoint sur un réseau multi-sauts sans-fil basé sur le paradigme SDN. Un algorithme basé sur la programmation linéaire en nombres entiers et un algorithme génétique sont également proposés pour l’allocation de liens virtuels point-à-multipoint sur un réseau sans-fil multi-radio, multi-canaux et multi-sauts basé sur SDN. Enfin, pour traiter le cas des services dynamiques point-à-multipoint, nous proposons un schéma de réallocation de ressources qui répond aux exigences changeantes tout en réduisant les interuptions de service. / The movement towards the digitalization of many industry sectors such as automotive, agriculture, transportation, city management, etc. is revealing the need for novel usages of point-to-multipoint network delivery services, such as massive delivery of software updates to objects, secure and reliable delivery of alert messages to population, etc. On another side, the softwarization of next generation networks, with amongst, the increasing adoption of Software Defined Networks (SDN) is bringing to the network the flexibility and programming capabilities that enable the support of point-to-multipoint delivery services in an efficient and cost-effective way. This PhD work contributes to the general problem of providing point-tomultipoint delivery services with Quality of Service (QoS) requirements in a multidomain SDN network. It also considers that some of the domains are wireless multi hop networks. First, a Steiner tree based network domain topology aggregation combined with a resource allocation heuristic algorithm is proposed to support point-to-multipoint delivery services that span multiple domains. Then, a generic topology discovery service is proposed for SDN based wireless multi-hop networks to let the SDN controller build and maintain a comprehensive view of the network with various node and wireless link attributes. From there, customized views can be exposed by the controller to network control applications, as, for instance, the application in charge of provisioning point-to-multipoint services on a SDN based wireless multi-hop network. An Integer linear programming based algorithm and a genetic algorithm are also proposed for the embedding of point-to-multipoint services on a SDN based multi-radio, multi-channel and multi-hop wireless network. Last, to address the case of dynamic point-to-multipoint services, we propose a resource reallocation scheme that meets the changing requirements while reducing service disruption.

Les machines électriques jouent un rôle très important dans la conversion d'énergie dans plusieurs applications et domaines. Les contraintes thermiques jouent ainsi un rôle indispensable dans la conception des machines électriques de plus en plus petites et performantes. En effet, la performance des machines électriques est limitée par les températures maximales admissibles dans certaines zones critiques telles que le bobinage, les aimants permanents et les roulements. Deux approches principales peuvent être utilisées pour étudier le comportement thermique de la machine: la méthode nodale ou le circuit à constantes localisées ou les modèles numériques. Dans notre étude, nous proposons d'appliquer la méthode nodale sur une machine électrique intégrée dans un environnement mécatronique complexe. Le modèle thermique développé de la machine est ainsi présenté avec ses différents éléments. En effet, un modèle précis dépend fortement de plusieurs paramètres thermiques tels que les coefficients d'échange convectif, les conductances de contact, les conductivités équivalentes du bobinage, et autres paramètres. En conséquence, des techniques d'analyse de sensibilité sont ensuite appliquées sur le modèle thermique pour identifier les paramètres d'influence significative sur les températures de la machine ainsi que pour la réduction de ce modèle. Ensuite, nous appliquons deux méthodologies d'identification des paramètres thermiques incertains sont développées et appliquées afin de recaler le modèle thermique de la machine. Cette étape permet la validation de ce modèle par rapport à des mesures thermiques sur une machine synchrone à aimants permanents internes installée sur un banc de caractérisation de machine électriques. Finalement, nous intégrons le modèle recalé dans une approche système mécatronique comportant les lois de commande de la machine ainsi que son convertisseur. Ceci permettra ainsi d'étudier l'influence de la température d'une machine électrique sur le système mécatronique complet. / Electric machines play an important role in power conversion in several applications and fields. With the increasing demand for designing lighter and more efficient machines and optimizing the existing structures, thermal analysis becomes a necessary; in fact, the performance of electric machines islimited by the allowable temperatures in many critical components like windings, permanent magnetsand bearings. Two main approaches can be employed in order to study the machine thermal behavior : the lumped parameter thermal network (LPTN) or numerical models. Considering low-computationtime-consuming and the possibility to be integrated in a mechatronics system design, the LPTN method is considered in our study. The latter is mainly applied on electric machine integrated in a complex mechatronics environment. The thermal network is presented along with the definition of the principal elements constituting this network. In fact, an accurate and reliable network strongly depends on many critical parameters like heat transfer coefficients, interface gaps, impregnation goodness, among others. For this reason, different sensitivity analysis techniques are carried out in order to, first, identify the significance of uncertainties in the evaluation of these parameters on machine temperatures and second, to reduce the thermal network. Next, we propose two optimization algorithm-based identification methodologies in order to calibrate results of the thermal network with measured temperatures obtained from a test-bench of a permanent magnet based integrated starter-generator machine. The calibrated model is then integrated in a mechatronics system consisting of an electric model of the electric machine, along with its control strategy and the power converter. This final study allows us to evaluate the impact of the machine temperature rise on the mechatronic system.

Le contexte d’étude est celui de la Compatibilité ÉlectroMagnétique (CEM). L’objectif de la CEM est, comme son nom l’indique, d’assurer la compatibilité entre une source de perturbation électromagnétique et un système électronique victime. Or, la prédiction de ces niveaux de perturbation ne peut pas s’effectuer à l’aide d’un simple calcul analytique, en raison de la géométrie qui est généralement complexe pour le système que l’on étudie, tel que le champ à l’intérieur d’un cockpit d’avion par exemple. En conséquence, nous sommes contraints d’employer des méthodes numériques, dans le but de prédire ce niveau de couplage entre les sources et les victimes. Parmi les nombreuses méthodes numériques existantes à ce jour, les méthodes Multi-Domaines (MD) sont très prisées. En effet, elles offrent la liberté aux utilisateurs de choisir la méthode numérique la plus adaptée, en fonction de la zone géométrique à calculer. Au sein de ces méthodes MD, la « Domain Decomposition Method » (DDM) présente l’avantage supplémentaire de découpler chacun de ces domaines. En conséquence, la DDM est particulièrement intéressante, vis-à-vis des méthodes concurrentes, en particulier sur l’aspect du coût numérique. Pour preuve, l’ONERA continue de développer cette méthode qui ne cesse de montrer son efficacité depuis plusieurs années, notamment pour le domaine des Surfaces Équivalentes Radar (SER) et des antennes. L’objectif de l’étude est de tirer profit des avantages de cette méthode pour des problématiques de CEM. Jusqu’à maintenant, de nombreuses applications de CEM, traitées par le code DDM, fournissaient des résultats fortement bruités. Même pour des problématiques électromagnétiques très simples, des problèmes subsistaient, sans explication convaincante. Ceci justifie cette étude. Le but de cette thèse est de pouvoir appliquer ce formalisme DDM à des problématiques de CEM. Dans cette optique, nous avons été amenés à redéfinir un certain nombre de conventions, qui interviennent au sein de la DDM. Par ailleurs, nous avons développé un modèle spécifique pour les ouvertures, qui sont des voies de couplage privilégiées par les ondes, à l’intérieur des cavités que représentent les blindages. Comme les ouvertures sont, en pratique, de petites dimensions devant la longueur d’onde, on s’est intéressé à un modèle quasi-statique. Nous proposons alors un modèle, qui a été implémenté, puis validé. Suite à ce modèle, nous avons développé une méthode originale, basée sur un calcul en deux étapes, permettant de ne plus discrétiser le support des ouvertures dans les calculs 3D. / The context of the study is the ElectroMagnetic Compatibility (EMC). Principal aim of the EMC is to ensure the compatibility between an electromagnetic perturbance source and an electronic device victim. Unfortunately, the perturbation levels prediction can not be made using an analytic formula, because the geometry which is generally complex for the interesting system, for example the field inside an aircraft’s cockpit. Therefore, we are contrained to use numerical methods, to be able to evaluate this coupling level between sources and victims. Among several existing numerical methods, Multi-Domains (MD) methods are very interesting. They offer to users the freedom to choose the most powerfull numerical method, in terms of the geometrical zone evaluated. With the MD methods, « Domain Decomposition Method » (DDM) has the avantage of decouplingeach of theses areas. Therefore, DDM is very interesting, compared to other methods, in particular on the numerical cost. ONERA keeps on developing this method, which has not stop showing his efficiency since several years, in particular in Radar Cross Section (RCS) and antennas. The objective of this study is to take the benefits of this method for EMC problems. Up to now, several EMC applications treated by the DDM code provided results strongly noisy. Even for with very simple electromagnetic cases, some problems remained without convincing explanations. This justifies this study. The aim of this thesis is to can be able to apply DDM formalism to EMC problems. Then, we have been induced to redefine a number of conventions which are involved in the DDM. Otherwise, we have developed a specific model for the apertures which are privilegied tracts of the coupling by the penetration of waves inside cavities (shieldings). As the apertures have in practice smaller dimensions compared to the wavelength, we have been interested to a quasistatic model which was developped, implemented and validated. Following this model, we have developed an original method, based on a two step calculation, able to do not discretize the apertures support in 3D computations.