Spelling suggestions: "subject:"multilayer""
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Structure of Bovine Liver Catalase Solved by Electron Diffraction on Multilayered CrystalsKulik, Victor 13 July 2005 (has links)
The high resolution structure of protein molecules and protein-protein complexes is important to investigate their functions. Today, large 3D or 2D crystals are required to obtain protein structures by X-ray crystallography or conventional Electron Microscopy, respectively. However, production of such crystals of good quality is a solely empirical procedure, which relies on screening numerous crystallization conditions. At the same time, multilayered protein crystals are often a by-product of attempts to grow 3D or 2D crystals and could be obtained more easily. So far, multilayered protein crystals have not been used in electron microscopy for structure determination, as the interpretation of an electron diffraction pattern is rather complicated. In this thesis we present the first protein structure bovine liver catalase at 4 Å resolution solved using electron diffraction data from multilayered crystals. 55 diffraction patterns (17 tilt series) were recorded and used for the reconstruction. The tilt geometry of each individual diffraction pattern was determined by a least-squares algorithm or Laue zone analysis to perform spot indexing. The phase problem was solved by molecular replacement. The influence of the missing data cone on the self-rotation function and interpretation of reconstructed map is discussed.
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Three-Dimensional Finite Element Modeling of Multilayered Multiferroic CompositesWang, Ruifeng 08 August 2011 (has links)
No description available.
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Abdu_dissertation_report.pdfAbdulrahman Mubarak Alanazi (15348496) 27 April 2023 (has links)
<p>Non-destructive characterization of multi-layered structures that can be accessed from only a single side is important for applications such as well-bore integrity inspection. Collimated beam ultrasound systems are a technology for imaging inside multi-layered structures such as geothermal wells. These systems work by using a collimated narrow-band ultrasound transmitter that can penetrate through multiple layers of heterogeneous material. A series of measurements can then be made at multiple transmit frequencies. However, commonly used reconstruction algorithms such as Synthetic Aperture Focusing Technique (SAFT) tend to produce poor quality reconstructions for these systems both because they do not model collimated beam systems and they do not jointly reconstruct the multiple frequencies.</p>
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<p>In this thesis, we first propose a multi-frequency ultrasound model-based iterative reconstruction (UMBIR) algorithm designed for multi-frequency collimated beam ultrasound systems. The combined system targets reflective imaging of heterogeneous, multi-layered structures. For each transmitted frequency band, we introduce a physics-based forward model to accurately account for the propagation of the collimated narrow-band ultrasonic beam through the multi-layered media. We then show how the joint multi-frequency UMBIR reconstruction can be computed by modeling the direct arrival signals, detector noise, and incorporating a spatially varying image prior.</p>
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<p>We also propose a ringing artifact reduction method for ultrasound image reconstruction that uses a multi-agent consensus equilibrium (RARE-MACE) framework. Our approach integrates a physics-based forward model that accounts for the propagation of a collimated ultrasonic beam in multi-layered media, a spatially varying image prior, and a denoiser designed to suppress the ringing artifacts that are characteristic of reconstructions from high-fractional bandwidth ultrasound sensor data.</p>
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<p>Results using both simulated and experimental data indicate that multi-frequency UMBIR reconstruction yields much higher reconstruction quality than either single frequency UMBIR or SAFT. In addition, our results demonstrate the capability of our RARE-MACE method to suppress ringing artifacts and substantially improve the image quality over single frequency UMBIR and SAFT.</p>
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POLYMER CRYSTALLIZATION IN DROPLETS AND CONFINED LAYERS USING MULTILAYERED FILMSLanghe, Deepak 30 January 2012 (has links)
No description available.
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3D Numerical Modeling Of Dry/Wet Contact Mechanics For Rough, Multilayered Elastic Plastic Solid Surfaces And Effects Of Hydrophilicity/Hydrophobicity During Separation With ApplicationsCai, Shaobiao 14 April 2008 (has links)
No description available.
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Investigation of Graphene Oxide Based Multilayered Capsules/Films for Drugs Delivery And Antimicrobial ApplicationsKurapati, Rajendra January 2013 (has links) (PDF)
Polyelectrolyte multilayer capsules fabricated by layer-by-layer (LbL) self-assembly technique consistsing of core-shell structure have emerged as potential drug delivery systems along with their applications in micro-reactors, cosmetics, vaccines and antimicrobial coatings. Various ligands and stimuli responsive entities can be incorporated into the core and shell of the capsules for targeted delivery and/or controlled release applications. Though multilayer capsules have been studied extensively as delivery systems, their utility for encapsulation of hydrophobic drugs and multiple drugs have not been explored in detail so far. Application of traditional polyelectrolyte capsules has several limitations, which renders them inapplicable for encapsulation of multiple drugs, hydrophobic drugs and also for releasing drugs on demand without addition of the external photothermal agents such as metal nanoparticles into the shells of the capsules.
Thus, in this thesis, an attempt has been made to develop novel multifunctional multilayered capsules to overcome the above mentioned limitations. We have formulated two novel methods to functionalize the core with cyclodextrin molecules and the shell of the capsules with two-dimensional material, graphene oxide (GO). The properties such as high surface area along with π bonds, broad NIR-absorption, superior photothermal conversion and antimicrobial activity of graphene oxide has been explored and it has been demonstrated that 2-D graphene oxide is unique compared to the regular polyelectrolytes. By functionalizing the shell of capsules with GO as one of the layer material, a simple and efficient way for encapsulating multiple drugs into core and shell of the capsules is achieved by utilizing the large surface area and amphiphilic nature of GO. Based on the unique optical absorption and photothermal conversion properties of GO, we have demonstrated a facile route for near-infrared (NIR)-laser triggered release with low laser power. In the second part, functionalization of the hollow core of the capsules has been functionalized using cylodextrin (CD)-incorporated CaCO3 porous sacrificial templates, where both CD-CaCO3 and CD-modified capsules are used as high efficient carriers for hydrophobic drugs. In the third part, synergistic antimicrobial therapy was achieved using composite graphene oxide/polymer LbL films by combining the intrinsic antimicrobial activity and photothermal conversion ability of graphene oxide and the results depicted superior antimicrobial activity towards E. coli. These composite films also can be used as efficient antimicrobial coatings on biomedical devices or implants.
The thesis has been divided into five chapters based on the individual works. In Chapter 1, a brief review on the history of LbL self-assembly, mechanism of self-assembly along with factors affecting the process have been discussed. Followed by a brief discussion about the fabrication of multilayered hollow capsules (core-shell structure), their applications in drug delivery and fabrication of multifunctional multilayered capsules through core and shell have been discussed. Finally, recent developments in LbL self-assembly and multilayered hollow capsules using carbon based materials (fullerenes, carbon nanotubes and graphene oxide) and their biomedical applications have been presented.
Chapter 2 deals with the study on fabricating multifunctional multilayered capsules for facile encapsulation of multiple drugs into the capsules, which is achieved by functionalizing the capsules with graphene oxide (GO) as one of the layer materials. The GO composite capsules exhibited unique permeability properties compared to traditional multilayered capsules made of two polyelectrolytes. Multiple drugs could be simultaneously encapsulated in the capsules in a simple and effective manner. These capsules were found to exhibit a “core-shell” loading property for encapsulation of dual drugs into the core and shell of the capsules respectively. In addition, the graphene oxide composite capsules showed excellent biocompatibility towards MCF-7 cells. This study is the first one that demonstrates the potential of hybrid polyelectrolyte capsules without the use of micelles or polymer-drug conjugates for multi-drug encapsulation.
Chapter 3 deals with the development of a facile route for near-infrared (NIR)-light triggered release of encapsulated drugs from the multilayered capsules via incorporation of graphene oxide (GO) into layer-by-layer (LbL) assembled capsules without addition of any external additives such as metal nanoparticles (NPs) or carbon nanotubes (CNTs) into the shells of the capsules. Till now, there is no report on light-responsive drug delivery system by utilizing the NIR-optical absorption properties of GO. Here, graphene oxide (GO) plays a dual role, serving as a structural component of LbL capsules as well as strong NIR-light absorbing agent, which efficiently converts absorbed light into heat. Upon NIR-laser irradiation, the microcapsules were opened in “point-wise fashion” due to local heating caused by laser irradiation. The rupturing mechanism of the capsules has been clearly demonstrated using confocal fluorescence microscopy and high resolution transmission electron microscopy. The light-triggering ability of these capsules has been applied successfully to release the encapsulated anticancer drug, doxorubicin.
Chapter 4 deals with simple and versatile simple routes for encapsulation of model hydrophobic drug. Encapsulation of hydrophobic drugs in pharmaceutical industries is always a big challenge due to limited number of available drug carrier systems and poor aqueous solubility of hydrophobic drugs. Here, by combining the special properties of cyclodextrins (CDs) with biodegradable inorganic calcium carbonate microparticles, the hybrid CD-CaCO3 mesoporous microparticles have been prepared for the first time. These CD-CaCO3 microparticles were utilized as sacrificial templates to prepare CDs-modified LbL capsules. We have demonstrated that both the hybrid CD-CaCO3 microparticles and CDs-modified capsules are potential carriers for encapsulation of model hydrophobic drugs (self-fluorescent coumarine and nile red dyes) with high loading efficiency using supramolecular host-guest interaction between entrapped CDs and hydrophobic dye molecules. Compared with other inorganic drug carrier systems (mesoporous silica), CaCO3 porous particles have better biocompatibility, biodegradability and cost-effective and without use of any organic solvents. Both these hybrid CD-CaCO3 microparticles and CDs-modified capsules can be good candidates for encapsulation of hydrophobic drugs without involving extreme chemical conditions for fabrication.
Chapter 5 deals with development of facile synergistic method for killing pathogenic bacteria by combining the intrinsic antimicrobial activity of graphene oxide (GO) and unique photothermal conversion property of GO into a single material. We fabricated composite LbL films of graphene oxide (GO) and poly(allylamine hydrochloride) (PAH) films. Antimicrobial activity of these GO composite films has been studied using Escherichia coli (E. coli) cells by varying number of deposited layers on glass slides (20 to 80 layers) and results suggest that by increasing the number of deposited layers, antimicrobial activity is also increased gradually. Based on the unique optical properties of GO, photothermal therapy have been carried out for killing of E. coli using GO composite films by varying number of deposited layers (20 to 80 layers) by irradiation of NIR-pulse laser at 1064 nm wavelength (Nd:YAG, 10 ns pulse, 10 Hz). The photothermal results revealed the enhanced antimicrobial activity compared to GO composite films alone without NIR-laser irradiation. The synergistic photothermal killing ability along with intrinsic antimicrobial activity of GO films results in much faster killing compared to films alone.
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[en] PSEUDO-ANALYTICAL MODELING FOR ELECTROMAGNETIC WELL-LOGGING TOOLS IN COMPLEX GEOPHYSICAL FORMATIONS / [pt] MODELAGEM PSEUDOANALÍTICA PARA FERRAMENTAS DE PERFILAGEM ELETROMAGNÉTICA EM FORMAÇÕES GEOFÍSICAS COMPLEXASGUILHERME SIMON DA ROSA 17 July 2017 (has links)
[pt] Esta tese apresenta um estudo sobre técnicas de modelagem numérica utilizadas na análise da propagação eletromagnética em formações geofísicas comumente encontradas na perfuração de poços de petróleo. O emprego de sensores eletromagnéticos adjacentes à broca de perfuração permite a inferência dos parâmetros constitutivos do solo ao redor do poço. Nos últimos anos, os avanços da tecnologia de perfilagem eletromagnética permitiram a modelagem em tempo real do problema, possibilitando direcionar a perfuração do poço a fim de maximizar a exploração de petróleo, gás, e outros hidrocarbonetos fósseis. Formações geofísicas complexas são predominantes neste tipo de problema, e geralmente são modeladas usando técnicas numéricas de força bruta como os métodos de diferenças finitas, dos elementos finitos ou dos volumes finitos. No entanto, estas técnicas têm um custo computacional relativamente alto em termos de memória e tempo de processamento. O avanço da tecnologia de perfilagem em tempo real requer
abordagens mais eficientes. Neste trabalho nós empregamos o método do casamento de modos combinado com uma série de características positivas dos métodos pseudoanalíticos conhecidos na literatura para obter uma técnica inédita que permite analisar poços direcionais com estratificações radiais e longitudinais em formações geofísicas anisotrópicas. A técnica proposta permite modelar problemas ainda não explorados, mas com motivação tecnológica iminente, como a propagação eletromagnética ao longo de poços curvados e a perfuração em camadas inclinadas em relação ao eixo axial do poço. Nós apresentamos uma série de resultados de validação que demonstram que a técnica introduzida neste trabalho pode modelar de forma acurada e eficiente sensores de perfilagem eletromagnética usados na exploração de petróleo e gás. / [en] This research presents a study on numerical techniques to model the electromagnetic propagation in geophysical formations commonly encountered in oil well drilling. The employment of electromagnetic sensors surrounding the drill bit allows inferring the constitutive parameters of the soil around the well. In recent years, advances in electromagnetic logging technology have enabled the real-time modeling of this problem. In this way, the drilling direction can be guided in order to maximize the exploitation of oil, gas, and other fossil hydrocarbons. The complex geophysical formations that are prevalent in this type of problem can be effectively handled using brute-force numerical techniques such as finite-differences, finite-elements and finite-volumes. However, these techniques suffer from relatively high cost in terms of both computer memory and CPU time. The advancement of real-time logging technology demands approaches that are more efficient than purely numerical methods. In this work, we employ the mode-matching
technique combining attractive features of the well-known pseudo-analytical approaches to obtain a new technique for analyzing directional well-logging tools in anisotropic formations with both radial and axial stratifications. The proposed technique allows to model problems not yet explored, but with a strong technological motivation, such as electromagnetic propagation along curved wells and drilling along inclined layers. We present a series of validation results showing that the novel technique introduced in this study
can model accurately and efficiently electromagnetic logging sensors used in oil and gas exploration.
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Studies On Preparation Of Poly(Vinyl Pyrrolidone) And Poly (Methacrylic Acid) Microcaopsules For Drug DeliveryKumar, K N Anil 01 1900 (has links)
There has been growing interest in designing and development of suitable micro or nano drug delivery system with the ability to target site specifically and release the payload in a predetermined fashion. Recently a new type of system called polyelectrolyte microcapsules and thin films have been proposed and developed for applications such as, biomedical devices to micro sensing and drug delivery. Owing to its advantages of mild preparation conditions, multifunctionality, with programmable characteristics and to encapsulate large amount of materials, it has shown immense potential.
In the present research, multilayer polyelectrolyte thin films composed of Poly(methacrylic acid) (PMA) and Poly (vinyl pyrrolidone) (PVP) were deposited on the flat substrates using layer by layer (LBL) technique. The film growth and its deconstruction under physiological conditions were characterized using UV Visible spectrophotometer and Scanning Electron Microscopy (SEM). Hollow microcapsules composed of PMA and PVP were also produced with the help of sacrificial silica template using the same LBL adsorption technique. After coating the desired number of PVP and PMA layers, the colloidal template was removed with a buffer system composed of Hydrofluoric acid (HF) and Ammonium fluoride (NH4F). The obtained capsules were characterized for its surface morphology using SEM and Atomic Force Microscopy (AFM). The hydrogen bonding in capsule formation was confirmed by Fourier Transform Infrared Spectroscopy (FTIR).
Encapsulation and release with the microcapsules was carried out using Rifampicin (Antitubercular drug) as a model drug. The interaction of empty and drug loaded capsules with Mycobacterium Smegmatis cell line was investigated. It was found that the empty capsules did not affect the cell growth indicating their biocompatibility. Confocal microscopy studies with Doxorubicin (anticancer drug), which is a naturally fluorescent molecule, showed the drug is indeed encapsulated inside the hollow capsule. From the above studies, it was concluded that polyelectrolyte capsules have the potential to be used for delivering drugs.
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Realaus laiko neardančiosios kontrolės signalų apdorojimo sistema / Real-time signal processing system for nondestructive testingKazanavičius, Vygintas 24 May 2005 (has links)
The employment of real time NDT systems has been spread widely last years. It is very important to control in real-time layer thicknesses of multi-layered materials during manufacturing process. In this work multi-layer thickness measurement digital signal processing methods are evaluated. Presented real-time nondestructive testing system and signal propagation model is a background for measurement algorithm analysis and development. In this work multi-layer thickness measurement problem is addressed with a different approach, by applying correlation functions on parts of the ultrasonic signal, thus reducing the overall computational complexity.
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Experimental and numerical modeling of the dissolution of delta ferrite in the Fe-Cr-Ni system : application to the austenitic stainless steels / Modélisation expérimentale et numérique de la dissolution de la ferrite delta dans le système Fe-Cr-Ni : application aux aciers inoxydables austénitiquesSaied, Mahmoud 24 May 2016 (has links)
La ferrite résiduelle δ est présente dans les microstructures de coulée des aciers inoxydables austénitiques. Elle résulte de la transformation incomplète δ→γ ayant lieu l'étape de solidification. Sa présence peut nuire à la forgeabilité à chaud des aciers inoxydables et peut conduire à la formation de criques de rives et de pailles en J lors du laminage à chaud des brames. Ce travail de thèse a pour but de comprendre les mécanismes de la transformation δ→γ à haute température dans les aciers inoxydables austénitiques via une modélisation expérimentale et numérique. La transformation a été étudié dans un alliage ternaire Fe-Cr-Ni coulé par lingot et de composition proche de celle des alliages industriels. Trois morphologies de ferrite ont été mises en évidence à l'état brut de solidification: lattes au bord du lingot, vermiculaire et lattes au centre. Leur cinétique de dissolution est étudiée à des températures allant de 1140°C à 1340°C et caractérisée en termes de fraction de ferrite et profils de composition du Cr et du Ni. La dissolution de la ferrite vermiculaire comprend trois étapes : une croissance initiale transitoire suivie par deux régimes de dissolution à haute puis à faible taux de transformation. D'un autre côté, il a été possible d'étudier la dissolution de la ferrite dans des microstructures multicouches élaborées par l'empilement de plaques de ferrite et d'austénite du système Fe-Cr-Ni et soudées à l'état solide par Compression Isostatique à Chaud puis réduits en épaisseurs par laminages successifs. L'étude et la caractérisation de la cinétique de dissolution de la ferrite est plus facile dans ces microstructures étant donnée la planéité initiale des interfaces δ/γ. L'analyse des résultats expérimentaux a été menée via le développement d'un modèle numérique, à interface mobile, de la transformation de phases δ→γ pilotée par la diffusion. La diffusion peut être traitée dans les géométries plane, cylindrique et sphérique. En guise de validation, le modèle a été utilisé pour analyser la dissolution de la ferrite dans les microstructures multicouches. Par la suite il a été appliqué au cas de la ferrite vermiculaire en usant d'une approche novatrice où la morphologie des dendrites est approximée par une combinaison de cylindres et de sphères. Malgré la simplicité des hypothèse sous-jacentes, le modèle a permis d'expliquer les mécanismes de croissance initiale et de changement de régime de dissolution. D'autre part, via une étude paramétrique, l'effet des données d'entrée a été étudié et les plus pertinentes d'entre eux en termes de prédiction quantitative ont été mises en avant, en particulier la description thermodynamique du digramme Fe-Cr-Ni, le gradient initial et la distribution des rayons des particules de ferrite. / Residual δ-ferrite is widely encountered in the as-cast microstructure of austenitic stainless steels. It stems from the incomplete high temperature solid-state δ→γ transformation occurring upon the solidification stage. Its presence has a detrimental effect the hot workability of stainless steels, leading to the formation of edge cracks and sliver defects during slabs hot rolling. This PhD work aims at bringing more understanding of the kinetics of high temperature δ→γ transformation in austenitic stainless steels via experimental and numerical modeling. The transformation was studied in a ternary Fe-Cr-Ni ingot-cast alloy with composition close to the industrial alloys. Three ferrite morphologies were identified: lathy at the edge of the ingot, vermicular and lathy at the center. Their dissolution kinetics were established at temperatures ranging from 1140°C to 1340°C and characterized in terms of ferrite fraction and Cr and Ni diffusion. The vermicular ferrite undergoes a transient growth followed by a high then a low rate dissolution regimes. On the other hand, ferrite dissolution was also studied in the multilayered microstructures. such microstructures were elaborated by alternating ferrite and austenite sheets of the Fe-Cr-Ni system, diffusion-bonded by Hot isostatic Pressing and reduced in thickness by successive rollings. Dissolution is easier to handle in such microstructures thanks to the initial planar δ/γ interfaces. Analysis of the experimental results were carried out with a numerical moving-boundary model of diffusion-controlled δ→γ transformation. Diffusion can be treated in the planar, cylindrical and spherical geometries. As a preliminary validation, the model was used to analyze kinetics of ferrite dissolution in the multilayered microstructures. It was then applied to the cast alloy using an original descriptive approach combining spheres and cylinders as equivalent morphology of dendritic ferrite. Although based on simplifying assumptions, the model was able to reproduce experimental results with satisfactory agreement. Mechanisms underlying the initial growth of vermicular ferrite and the transition in dissolution regimes were outlined. The effect of a wide range of input parameters has been considered and relevant parameters for quantitative calculations were brought to light, such as thermodynamical descriptions of the Fe-Cr-Ni system, composition gradients and distribution of ferrite's radii.
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