Inaug.-diss.--Bern.

No description available.

The traditional herbal medicinal product Myrrhinil-Intest® is a fixed herbal combination, which is marketed in Germany since 1959 and applied in medical practice for the treatment of gastrointestinal disorders such as functional diarrhea, irritable bowel syndrome and inflammatory bowel disease. It contains myrrh, which is described as the oleo-gum resin from mainly Commiphora molmol Engler (Burseraceae), coffee charcoal, which are the milled roasted to blackening outer seed parts of green dried Coffea Arabica Linné (Rubiaceae) fruits and chamomile flowers - the flower heads of Matricaria recutita Linné (Asteraceae). The clinical effectiveness of Myrrhinil-Intest® for the treatment of various gastrointestinal disorders was demonstrated in several clinical studies and is described in various experience reports, however its pharmacological profile is not fully elucidated. Within the present study the spasmolytic and anti-inflammatory potential of the components myrrh, chamomile and coffee charcoal was investigated. Therefore pharmacological, histological and molecular biological methods were utilised. Spasmolytic activity was characterised using isometric tension measurement with rat isolated small intestinal preparations. Anti-inflammatory potential was assessed with different methods using isolated rat small intestinal preparations and immune cell lines. Inflammation was induced with TNBS and LPS respectively. Additionally, the influence of the herbal components on the gene expression profile of native human macrophages after LPS/IFNγ stimulation was determined by microarray gene expression analysis. Chamomile flower and myrrh exerted spasmolytic effects, whereby the more pronounced spasmolytic effects of myrrh were mediated via calcium channel blockade. Myrrh and chamomile flower exerted anti-inflammatory effects.

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No. of bitstreams: 2 Dissertação - Laís Lima Nabuco Araújo - 2014.pdf: 1037857 bytes, checksum: c46e9ea58576efaed7579fbccc51c41e (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-02-26 / Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG / MORPHOPHYSIOLOGICAL CHARACTERISTICS, PRODUCTION AND COMPOSITION OF ESSENTIAL OIL IN LEAVES OF Tetradenia riparia (Hochst) Codd-LAMIACEAE CULTIVATED AT DIFFERENT LEVELS OF SHADING. Tetradenia riparia ( Hochst ) Codd- Lamiaceae, popularly known as incense, is used for diseases like malaria, angina, gastroenteritis , headaches , bronchitis , among other diseases. Its essential oil contains approximately 200 components. The diterpenoids have antimicrobial, antispasmodic and antitricomonas activity. Considering the importance of light on plant growth and development the present study aims to evaluate morphological and physiological characteristics and production and composition of essential oil of Tetardenia riparia cultivated at different levels of shading. For this, seedlings were propagated by stem cuttings and grown in mini-greenhouses completely covered with black polyethylene screens produced to provide 30%, 50% and 80 % of levels shading and also grown under open sky. Leaves were evaluated after 150 days of treatment. The plants under open sky had greater foliar blade thickness. Shaded plants had higher leaf area. There were no significant differences in stomata and trichomes in the abaxial epidermis. The highest oil yield occurred in plants grown with 30 % and 50 % shading treatments. The major component of the essential oil, regardless of the treatment, was the sesquiterpenic hydrocarbon 14 - hydroxy -9 - epi - (E) - caryophyllene. Many chemical compounds of the essential oil alter its content according to treatments and other compounds were only observed at specific levels of shading. The total content of chlorophyll, chlorophyll “a” and carotenoids was higher in 50% and 80% treatment shading. The levels of chlorophyll “b” and a / b ratio did not change in different treatments. The different treatments did not change values of ash. The lipid content was lower in the treatment of 80% and increased protein and nitrogen content in 50% and 80% treatment shading. / CARACTERÍSTICAS MORFOFISIOLÓGICAS, PRODUÇÃO E COMPOSIÇÃO DE ÓLEO ESSENCIAL EM FOLHAS DE Tetradenia riparia (Hochst) Codd- LAMIACEAE CULTIVADA EM DIFERENTES NÍVEIS DE SOMBREAMENTO. Tetradenia riparia (Hochst) Codd- Lamiaceae, conhecida popularmente como incenso, é uma planta empregada para doenças como malária, angina, gastroenterites, dores de cabeça, bronquite, dentre outras doenças. Seu óleo essencial contém aproximadamente, 200 componentes. Os diterpenóides apresentam atividade antimicrobiana, antiespasmódica e antitricomonas. Considerando a importância da luz sobre o crescimento e desenvolvimento das plantas, o presente estudo tem como objetivo avaliar características morfofisiológicas, produção e composição de óleo essencial de Tetradenia riparia cultivada em diferentes níveis de sombreamento. Para isso, as mudas foram propagadas por estaquia e cultivadas em mini-casas de vegetação completamente cobertas com telas de polietileno pretas produzidas para proporcionar níveis de sombreamento de 30%, 50% e 80% e também a pleno sol o que corresponde aos tratamentos. Folhas foram avaliadas após 150 dias de tratamento. As plantas submetidas a pleno sol tiveram maior espessura do limbo foliar. As plantas cultivadas à sombra tiveram maior área foliar. Não houve diferenças significativas na densidade de estômatos e tricomas na epiderme abaxial. O maior rendimento de óleo essencial ocorreu nas plantas cultivadas nos tratamentos de 30% e 50% de sombreamento. O componente majoritário do óleo essencial das folhas, independente dos tratamentos a que as plantas foram submetidas foi o hidrocarboneto sesquiterpênico 14-hidróxi-9-epi-(E)- cariofileno. Muitos compostos químicos do óleo essencial alteraram o seu teor em função dos tratamentos e outros compostos só foram observados em níveis específicos de sombreamento. O teor de clorofila “a”, total e carotenoides foram maiores nos tratamentos de 50% e 80% de sombreamento. Os teores de clorofila “b” e razão a/b não sofreram alterações nos diferentes tratamentos. Os diferentes níveis de luminosidade não alteraram valores de cinzas. O teor de lipídios foi menor no tratamento de 80% e o teor de proteínas e nitrogênio aumentou com 50% e 80% de sombreamento.

The traditional herbal medicinal product Myrrhinil-Intest® is a fixed herbal combination, which is marketed in Germany since 1959 and applied in medical practice for the treatment of gastrointestinal disorders such as functional diarrhea, irritable bowel syndrome and inflammatory bowel disease. It contains myrrh, which is described as the oleo-gum resin from mainly Commiphora molmol Engler (Burseraceae), coffee charcoal, which are the milled roasted to blackening outer seed parts of green dried Coffea Arabica Linné (Rubiaceae) fruits and chamomile flowers - the flower heads of Matricaria recutita Linné (Asteraceae). The clinical effectiveness of Myrrhinil-Intest® for the treatment of various gastrointestinal disorders was demonstrated in several clinical studies and is described in various experience reports, however its pharmacological profile is not fully elucidated. Within the present study the spasmolytic and anti-inflammatory potential of the components myrrh, chamomile and coffee charcoal was investigated. Therefore pharmacological, histological and molecular biological methods were utilised. Spasmolytic activity was characterised using isometric tension measurement with rat isolated small intestinal preparations. Anti-inflammatory potential was assessed with different methods using isolated rat small intestinal preparations and immune cell lines. Inflammation was induced with TNBS and LPS respectively. Additionally, the influence of the herbal components on the gene expression profile of native human macrophages after LPS/IFNγ stimulation was determined by microarray gene expression analysis. Chamomile flower and myrrh exerted spasmolytic effects, whereby the more pronounced spasmolytic effects of myrrh were mediated via calcium channel blockade. Myrrh and chamomile flower exerted anti-inflammatory effects.

Le but de la thèse est d’analyser l’importance idéologique et religieuse des expéditions égyptiennes au pays de Pount, particulièrement sous la XVIIIe dynastie. La 1ère partie (chapitres 1-4) présente en détail les sources relatives aux expéditions pountites et des produits en rapportés. Bien que la prééminence soit réservée aux sources de la XVIIIe dynastie, les documents datant d’autres périodes ont été aussi inclus dans les analyses. La 2e partie (chapitres 5-8) constitue une synthèse des conclusions formulées en résultat des analyses faites dans la 1ère partie. De cette façon, l’auteur examine les divers toponymes relatifs au pays de Pount (chapitre 5), le problème de la localisation exacte de Pount (chapitre 6), le caractère des expéditions pountites en comparaison aux autres types des missions étrangères égyptiennes (chapitre 7) aussi bien que les divers liens entre le pays de Pount et la religion et l’idéologie du pouvoir en Égypte (chapitre 8). L’auteur conclut que Pount jouait un rôle crucial dans l’idéologie du pouvoir égyptienne étant particulièrement associé au dieu solaire aussi bien qu’à la déesse Hathor. Les produits pountites étaient extrêmement importants tant pour le culte funéraire royal et privé que pour le culte divin rendu dans les temples. C’est pourquoi l’organisation d’une expédition pountite était un devoir du roi qui, selon les croyances égyptiennes, obéissait à un ordre direct du dieu solaire. En plus, aux diverses époques on peut percevoir une volonté de rétablir les contacts avec Pount afin de restaurer l’ordre naturel après une période de la crise, ce qui est particulièrement visible sous le règne d’Hatchepsout de la XVIIIe dynastie. / The aim of the dissertation is to analyse the religious and ideological significance of the ancient Egyptian Punt expeditions, particularly in the 18th dynasty. In the first part (chapters 1-4) the sources concerning the ancient Egyptian Punt expeditions as well as the products imported to Egypt in their result were discussed in detail. Although the pre-eminence is given to the 18th dynasty sources, documents from other periods were also included in the analysis. The second part (chapters 5-8) constitutes a synthesis of the conclusions drawn in result of the analysis of the sources of the first part. Therefore, the author discusses various toponyms related to the land of Punt (chapter 5), the problem of Punt’s exact location (chapter 6), the character of the Punt expeditions in comparison with other foreign missions (chapter 7) as well as various links between the land of Punt and Egyptian religion and ideology of kingship (chapter 8). The author concludes that the land of Punt played an important role in the Egyptian ideology of kingship being especially associated with the sun-god as well as with the goddess Hathor. Puntite products were crucial for both royal and private funerary cult as well as for the divine cult carried out in the temples. This is why the organisation of a Punt expedition was a duty of the Egyptian king, who, according to Egyptian beliefs, was obeying a direct order of the sun-god. Moreover, in various periods we may observe a wish to re-establish the contacts with Punt in order to restore the natural order of things after a period of crisis, which is especially visible in the reign of Hatshepsut of the 18th dynasty.