Life on Earth is currently experiencing a sixth species extinction, where urbanization, as one of the main driving forces, fragments and causes losses of near-urban habitats. At the same time, a rich variety of habitats can be found in urban landscapes that may harbour biodiversity. Knowledge of how urban ecosystems develops over time is fundamental for application of relevant nature conservation measures that promote species richness. This study aims to shed light on the importance of age in urban residential yards for the species richness of native herbaceous plants and of birds. The study was carried out in Linköping during the summer of 2023, and it included surveys of native herbaceous plants and birds in three area types (natural area, old and young residential yards). In total, 114 species of native herbs and 34 species of birds were found. Sites in the natural area had (mean ± SE) 25,8 (± 1,8) and 6,6 (± 0,7) species of plants and birds respectively, whereas the corresponding values for old residential yards were 23,2 (± 1,2) and 5,7 (± 0,5) and for young residential yards 19,9 (± 1,3) and 3,3 (± 0,7). Jaccard's coefficient showed that the largest proportion of shared species occurred between ‘old’ and 'young' residential yards. Overall, the results of my study showed some support for my prediction that age of residential yards is important for species richness of native herbaceous plants and birds. The results indicate that urban environments (old and young residential yards) harbour a selection of species that can withstand anthropogenic disturbance. / Livet på jorden upplever ett sjätte artutdöende där urbaniseringen, som en av de främsta drivkrafterna, fragmenterar och orsakar förluster av stadsnära habitat. Samtidigt finns en rik variation av livsmiljöer i stadslandskap som hyser en biologisk mångfald. Kunskap om hur urbana ekosystem utvecklas över tid är grundläggande för tillämpning av relevanta naturvårdsåtgärder i staden som främjar artrikedom. Denna studie syftar till att belysa betydelsen av urbana bostadsgårdars ålder för artrikedomen hos inhemska örtartade växter och fåglar. Studien genomfördes i Linköping under sommaren 2023 och omfattade inventeringar av inhemska örtartade växter och fåglar i tre områdestyper (naturområde, äldre och unga bostadsgårdar). Totalt hittades 114 arter av inhemska örter och 34 arter av fåglar. Naturområdet hade (medelvärde ± SE) 25,8 (± 1,8) respektive 6,6 (± 0,7) arter av växter och fåglar, medan motsvarande värden för äldre bostadsgårdar var 23,2 (± 1,2) och 5,7 (± 0,5) och för unga bostadsgårdar 19,9 (± 1,3) och 3,3 (± 0,7). Jaccards koefficient visade att den största andelen överlappande arter förekom mellan ’äldre’ och ’unga’ bostadsgårdar. Sammantaget visade resultaten av min studie ett visst stöd för min förutsägelse att bostadsgårdarnas ålder är av betydelse för artrikedomen hos inhemska örtartade växter och fåglar. Resultaten tyder på att stadsmiljöer (äldre och unga bostadsgårdar) hyser ett urval av arter som tål antropogena, av människan orsakade, störningar.

Biodiversity is being lost around the world and anthropogenic activities, like non-native species introductions and urbanization, are among the leading causes of decline. Broadening our understanding of human-driven impacts on biodiversity can lead to more effective solutions and contribute towards stemming biodiversity losses. In this thesis, I explored the impacts of non-native species introductions and urbanization on butterfly-plant communities. I evaluated potential factors influencing the range expansion of a newly introduced species (Chapter 1), examined the role of urbanization in structuring biological communities (Chapter 2), and identified important ecological interactions between native and non-native species (Chapter 3). In my first chapter, I found that the newly introduced European Common Blue butterfly (Polyommatus icarus) was more abundant in urban and disturbed habitat that was unmown and where their preferred larval host plant (Lotus corniculatus), a non-native species, was present. I also found that P. icarus was not a strong flier relative to other butterfly species, suggesting that adult dispersal may not be a driving factor in range expansion. Instead, this species could expand its range in the future by colonizing habitats in developed regions across Canada given the association I found between P. icarus and disturbed habitat. In my second chapter, I found that urbanization led to the biotic homogenization of butterfly communities around Montréal, QC, favouring a few, highly abundant, non-native species. Homogenization was detected at both the taxonomic and functional levels and was driven by increases in P. icarus abundance in the more urban sites. In my third chapter, I found that non-native plants were well integrated into native butterfly nectar diets in an at-risk oak savanna ecosystem on Vancouver Island, BC and that usage of non-native plants increased when they were more available during the season. I also found that native butterflies visited non-native plants more often than flower availability predicted on its own, suggesting that butterflies may even prefer non-native nectar. Findings from my thesis research broaden our understanding of the ways in which butterfly communities can be influenced by human activities like urbanization and the introduction of non-native species, informing conservation efforts and directing future research needs.

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Over the last 3 decades, freshwater aquaculture has become one of the most important food industries. However the constant introduction of a reduced number of very successful species for aquaculture has been identified as one of the main activities related to the alarming decline of fish biodiversity worldwide. This issue has raised awareness amongst the scientific community, governmental authorities and the general public towards freshwater fish biodiversity. This new awareness has promoted the development of “green” markets and environmentally friendly strategies, aiming for a reliable production of protein sources. The development of native species aquaculture has been presented as a strong alternative for sustainable aquaculture and the protection of biodiversity. However, it seems clear that unplanned native species aquaculture developments can be as detrimental on local biodiversity as the introduction of exotic fish, if not more dangerous. Therefore, the advantages and disadvantages of native species aquaculture have to be clearly analysed before any aquaculture development. This study aimed to establish a philosophical background regarding the use of native fish species in aquaculture in contrast to the introduction of exotic species that may compete for a similar niche as food in local markets. The main ecological impacts that exotic fish species may have on natives, such as competition, predation, and hybridization were discussed. In addition, a well planned native species Aquaculture Strategy for the Protection of Biodiversity was produced, at catchment level, within a Geographic Information System (GIS). For the development of the native species aquaculture strategy in central Mexico, four species of Atherinids (Chirostoma estor, C. Jordani, C. promelas and Atherinella balsana) and two species of native Ictalurids (Ictalurus balsanus and Ictalurus dugesii) were included in this study. These six species are relatively new to aquaculture and they were selected on the basis of their importance in local fisheries and markets in their native basins of the Lerma-Santiago and Balsas rivers. Both of these basins are of great importance in central Mexico, not only because of their biodiversity but also because of their high human population densities and socio-economic status. The use of Geographic Information Systems was a fundamental factor in the development of the native species aquaculture strategy at catchment level, consisting of site suitability models (SSM) for each species in their corresponding native catchments. Overall, SSM identified 13,916 km2 and 11,178 km2 highly suitable for aquaculture of the studied Atherinids and Ictalurids respectively, based on Water, Soil and Terrain, Infrastructure and Risk sub-models. A set of predictive species distribution models (PSDM), which related ecological characteristics for each studied species with relevant environmental and topographic parameters into a GIS, were also produced. Such models were developed for the establishment of potential natural ranges of distribution for each species, as well as their potential to become exotic in new environments, as a potential for invasion model (PI). Based on a partial verification, both PSDM and PI models produced results that were satisfactorily consistent with the known distribution of each modelled species. The combination of SSM and PSDM produced an Aquaculture Strategy for the Protection of Biodiversity model (ASPB) which identified the most environmentally friendly suitable areas for aquaculture sites. In contrast, the combination of the SSM with PI models into an ASPB model identified the site suitability potential for non-native species that are genetically close to native ones, in an attempt to reduce the known impacts that exotic species have on local biodiversity. In this way the ASPB model identified 7,651 km2 suitable for aquaculture of I. balsanus in its native Balsas basin and 15,633 km¬2 suitable for aquaculture of the non-native I. dugesii. ASPB models were produced for all the studied species. The final results were used to produce a set of guidelines for the development of sustainable aquaculture of native species at catchment level that cover genetic and ecological implications, as well as a well planned decision making tool produced in a GIS.

Grasses are widely used in revegetation to control erosion, build soil and maintain habitat. In northeast British Columbia, non-native grass species are commonly seeded to reclaim industrially disturbed sites. Widespread concern about degradation of biodiversity and key ecological processes has led to increasing value placed on native species and management practices leading to a more resilient landscape. I undertook this study to fill the restoration knowledge gap relating to native grasses in northeast BC. I did an extensive inventory of grasses on 217 sites in 2007, 2008 and 2009. Functional traits were measured in the field and in a greenhouse growth experiment. I found ninety-nine grass species occuring in the region, 70% of which are native. The number, proportion and extent of non-native grasses are increasing and four of these – Poa pratensis, Festuca rubra, Bromus inermis, and Phleum pratense represented almost a quarter of all occurrences. Several native species were common throughout the region: Calamagrostis canadensis, Leymus innovatus, Elymus trachycaulus, Poa palustris and Agrostis scabra. Other native species, including Festuca altaica, Koeleria macrantha, Pascopyrum smithii, and Schizachne purpurascens, strongly favoured intact habitats. Elevation, soil moisture regime, proportion of bare ground, and land use were significant factors related to local grass species composition and abundance. Agrostis scabra, Alopecurus aequalis, Beckmannia syzigachne, Bromus ciliatus, Cinna latifolia, Deschampsia cespitosa, Elymus alaskanus, Elymus trachycaulus, Festuca saximontana and Hordeum jubatum grew commonly on severely damaged well sites. Field measurements for Specific Leaf Area (SLA) and Leaf Dry Matter Content (LDMC) of 11 species showed an inverse correlation. Bromus ciliatus, Bromus pumpellianus, and Elymus trachycaulus had high SLA/low LDMC linked to rapid growth, whereas Festuca altaica, Deschampsia cespitosa, and Calamagrostis stricta had low SLA/high LDMC linked to slow growth and persistence. In the greenhouse experiment, Poa palustris, Cinna latifolia and Bromus ciliatus produced the most overall biomass and Pascopyrum smithii and Poa palustris produced the greatest aboveground biomass. Calamagrostis stricta, Poa palustris, Elymus glaucus, Leymus innovatus and Pascopyrum smithii exhibited clonal growth. Beckmannia syzigachne, Bromus ciliatus Cinna latifolia produced viable seed during the 135-day experiment. Considering all attributes five native species, Calamagrostis canadensis, Elymus trachycaulus, Poa palustris, Leymus innovatus, and Agrostis scabra are recommended for general restoration use in northeast B.C. Other native species show promise when matched to particular site conditions, including Alopecurus aequalis, Arctagrostis latifolia, Beckmannia syzigachne, Bromus ciliatus, Calamagrostis stricta, Cinna latifolia, Deschampsia cespitosa, Elymus glaucus, Festuca saximontana, Glyceria striata, Hordeum jubatum, Koeleria macrantha, Pascopyrum smithii, Poa alpina, Schizachne purpurascens and Trisetum spicatum. This information will be valuable to land managers interested in moving beyond reclamation to ecological restoration of sites disturbed by oil and gas development. Developing practices that are environmentally sound and socially acceptable requires ongoing botanical inventory. Plant traits may be useful in matching species to site conditions and restoration goals. Policy recommendations include phasing in of requirements to use native seed while restricting the use of agronomic species, promoting natural colonization, and supporting a native seed industry.

The aim of this thesis was to verify the success of previous reintroduction of noble crayfish (Astacus astacus) in main streams and selected reservoirs in the protected area Třeboňsko. Further expansion took place monitoring invasive signal crayfish (Pacifastacus leniusculus) and spiny cheek crayfish (Orconectes limosus) in these streams. Both are a carrier of crayfish plague. Thus, where they already exist, would not make sense to try to strengthen further the population of noble crayfish.

The present work had objective to analyze the economic-financial viability of aquaculture projects investment in cages for native species (jundiá, pacu and curimba). Aquaculture/fishculture inside of agribusiness has been presenting a growing interest and it should continue like this next decades, tends in view the stagnation in the fish offer originating from of the capture and of the growing increase in the demand for food with the increment of the world population. The increase of the consumption of fish and the progress of the aquaculture demand information for taking of decision about the economic viability of the enterprises in this area, mainly to native species. The investors, producers, technicians, fomentation organs and other institutions affect to the area, need safe information for taking decision about economic-financial viability of aquaculture/fishculture projects. In this sense this work verified that the production of native species in cages in the reservoir of Itaipu is unviable for the species jundiá (Rhamdia quelen) and curimba (Prochilodus lineatus), and viable for the Pacu (Piaractus mesopotamicus), which presented better adaptive to the production system and the climatic conditions. Analysis of Investment Return, Rate of Return, profitable Index, Internal Return Rate (IRR), Net Present Value (NPV) and Payback Period, all favorable ones were shown positively, with positive probability of occurrence ranging between 5% to 95% (90%) of the return of investment in a period among two (2) for three (3) years for the Monte Carlo simulation method. / O presente trabalho teve como objetivo analisar a viabilidade econômico-financeira de investimento em projetos aqüícolas, especificamente em tanques-redes para espécies nativas (jundiá, pacu e curimba). A atividade aqüícola dentro do agronegócio tem apresentado um interesse crescente e assim deve continuar nas próximas décadas, tendo em vista a estagnação na oferta de pescado oriundo da captura e do crescente aumento na demanda por alimento com o incremento da população mundial. O crescimento do consumo de pescados e o avanço da aqüicultura demandam informações para tomada de decisão sobre a viabilidade econômica dos empreendimentos nesta área, principalmente no que se refere às espécies nativas. Os investidores, produtores, técnicos, órgãos de fomento e demais instituições afetas à área necessitam de informações seguras para tomada de decisão relativa a viabilidade econômica-financeira de projetos aqüícolas. Neste sentido este trabalhou verificou que a produção de espécies nativas em tanques-rede no reservatório de Itaipu se mostrou inviável para as espécies jundiá (Rhamdia quelen) e curimba (Prochilodus lineatus), e viável para a espécie Pacu (Piaractus mesopotamicus), a qual apresentou melhor adaptabilidade ao sistema de produção e as condições climáticas. Para a espécie pacu, a análise de Retorno do Investimento, da Taxa de Retorno, Índice de Lucratividade, Taxa Interna de Retorno (TIR), Valor Presente Líquido (VPL) e Período de Retorno do Investimento, se mostraram todos favoráveis positivamente, com probabilidade positiva de ocorrência variando entre 5% a 95% (90%) do retorno do investimento em um período entre dois (2) a três (3) anos pelo método de simulação de Monte Carlo.

Human activities are increasing the occurrence of megafires that have the potential to alter the ecology of forest ecosystems. The objective of this study was to understand the impact of a 610 km2 megafire on patterns of forest regeneration and herbivory of three forest types (aspen/fir, oak/maple, and pinyon/juniper) within the burn scar. Sapling density, meristem removal, and height were measured across a transect network spanning the area of the burn scar over three years from 2019-2021. The network consisted of 17 burned/unburned transect pairs in adjacent burned/unburned forest stands (6 aspen/fir, 5 oak/maple, and 6 pinyon/juniper). Species that regenerated through sprouting generally responded positively to fire while regeneration from seed showed very little post-fire response. Browse pressure was concentrated on deciduous tree species and tended to be greater in burned areas but the effect diminished over time. Meristem removal of sprouting species was below the critical threshold resulting in positive vertical growth across years. Our results indicate that forest regeneration within the megafire scar was generally positive and experienced sustainable levels of ungulate browsing that are likely to result in forest recruitment success. Novel fire regimes are becoming increasingly common and megafires have burned across ecotonal boundaries across multiple forest types. Plant community structure and composition may be critically affected by changing fire regimes. Our objective was to investigate how a megafire that burned across multiple forest types impacted understory plant community assembly and biodiversity. Paired vegetation transects were installed in burned and unburned areas across aspen/fir, oak/maple, and pinyon/juniper forests within the 2018 Pole Creek Megafire burn scar. Percent cover of understory plants was measured in the summer of 2022 and plants were identified to the species level. Richness and diversity indices were then calculated and analyzed using mixed effects models. Fire decreased species richness of the aspen/fir forest understory and increased plant cover in pinyon/juniper forests, while not significantly impacting oak/maple understories. The significant effects of fire were largely driven by changes in forb species. Fire decreased the richness of native plants in aspen/fir forests but increased the richness of non-native plants in oak/maple and pinyon/juniper forests. Non-native plant abundance also increased in post-fire pinyon/juniper forests. Our results suggest that forest understory communities show variable responses to megafires that burn across multiple forest types with important implications for post-fire plant community structure, diversity, and invasibility. Large mammal herbivores (ungulates) are increasing in number and spreading into novel habitats throughout the world. Their impact on forest understory plant communities is strong and varies by herbivore, plant growth form, and season. The objective of this study was to determine the individual and collective herbivory impacts of native versus domestic ungulates on the understory plant community composition of post-fire aspen forests. Four-way fencing treatments were installed in 2012 to separate ungulate species, and Daubenmire frames were used to collect percent cover estimates for each understory plant species. Vegetation data were later used to calculate richness and diversity indices. Total understory plant cover, richness, and diversity were not significantly impacted by the herbivory fencing treatment. However, woody plant species' percent cover was 90% greater in full ungulate exclusion plots than in the fenceless controls. Herbivores likely targeted woody plant species due to their high nutrient levels that last longer into the winter than those of forb or graminoid species. Herbivory treatment did not affect non-native species. Our results indicate that herbivore fencing can protect forest understory plant communities, particularly the woody species. Successful regeneration of woody species can benefit the diversity of the entire understory plant community and preserve forest structure.

Biological invasions are one of the main factors responsible for the imperiled status of freshwater ecosystems, but much remains to be learned about their indirect effects on native communities. The first part of this dissertation examines community effects of long-term efforts to selectively control invasive Eurasian watermilfoil. Results of the first study show that native plants immediately recolonized treated areas and habitat complexity was unaffected. Fish community was not influenced by invasive plant control. Macroinvertebrate communities were highly variable and part of their variability could be explained by plant community attributes. Both fish and macroinvertebrates used invasive watermilfoil, which emphasizes the need for timely restoration of native macrophytes to mitigate for lost habitat. Because fish and macroinvertebrates were more affected by complexity than other attributes of plant assemblage, reestablishment of habitat complexity appears to be a promising restoration strategy. The second study, which examined species interactions after watermilfoil control, found that fish feeding activity was not correlated with invasive plants or habitat complexity and that invasive macrophyte control did not affect characteristics of fish feeding investigated. The relationship between fish and macrophytes was further explored in the context of interactions between an invasive piscivore and its native prey. First, I examined the prey naiveté hypothesis with non-native peacock bass in Paraná River, Brazil. Prey responded to visual and chemical cues of peacock bass and displayed avoidance behaviors similar to those observed with a native predator, meaning that lack of recognition was not responsible for the observed vulnerability of native species to this introduced predator. After confirming lack of naiveté, I assessed direct and indirect effects of this non-native predator on native prey. Peacock bass had no indirect effects on its prey feeding activity. Macrophyte type did not affect indirect predator-prey interactions, whereas direct predator effects slightly decreased in the presence of aquatic vegetation. I discuss implications of these findings for native biodiversity and convene other potential explanations for the observed effects of peacock bass. Both projects contribute to our understanding of the relationship between aquatic plants and their animal communities and effects of invasive species in freshwater habitats.