>Magister Scientiae - MSc / Different chlorhexidine (CHX) preparations and formulations are available in local markets. Some preparations contain anti-discoloration systems (ADS), additional antimicrobials like cetylpyridinium chloride (CPC), or alcohol. The aim of this study was to compare the antimicrobial efficacies of 3 different CHX preparations (Corsodyl®, Curasept® and GUM® Paroex®)

Die vorliegende klinische Studie verfolgte das Ziel das Universaladhäsiv 3MTM ScotchbondTM Universal (SBU) klinisch (FDI-Kriterien) und mit quantitativer Füllungsrandanalyse (QRA) über einen Zeitraum von 36 Monaten zu evaluieren und die beiden Analysemethoden miteinander zu vergleichen. Bei 22 Patienten wurden je 4 nichtkariöse zervikale Zahnhalsdefekte (NCCL) plastisch mit dem Komposit Filtek™ Supreme XTE restauriert. Das Universaladhäsiv 3MTM ScotchbondTM wurde in den drei Konditionierungsmodi self-etch (SE), selectiveenamel-etch (SEE) und etch-and-rinse (ER) angewendet. Als Referenzstandard diente das Etch-and-Rinse-Adhäsiv OptiBond™ FL (OFL). Die Füllungen wurden nach 14 Tagen (t1, Baseline), sechs Monaten (t2), zwölf Monaten (t3), 24 Monaten (t4) und 36 Monaten (t5) klinisch bewertet und parallel zur Herstellung von Repliken abgeformt. Nach 36 Monaten wurden aus den 22 Patienten 11 Patienten für die quantitative Füllungsrandanalyse (QRA) zufällig ausgewählt. Die Gruppenvergleiche erfolgten mit dem McNemar- (Klinik) und dem Friedman-/Wilcoxon-Test (QRA). Klinisch wurde ab dem sechsten Monat (t2) und zu jedem weiterem nachfolgenden Kontrollzeitpunkt (t3-t5) eine höhere kumulative Fehlerrate in der Kontrollgruppe OFL (23,8 %), als in den Gruppen SBU-SE (4,8 %), SBU-ER (0 %) und SBU-SEE (0 %) festgestellt. Bereits 14 Tage nach Füllungslegung (t1) wurden in den SBU-Gruppen weniger marginale Spaltformationen nachgewiesen als in der Kontrolle. Nach sechs Monaten (t2) wies die SBU-SE-Gruppe den größten Anteil an Randspalten aller Gruppen auf. Insgesamt zeigen in den SBU-Gruppen im Vergleich zu den OFL-Füllungen sowohl die klinischen als auch die QRA-Parameter ein geringeres Verbundversagen an der Zahnhartsubstanz-Komposit-Grenze an. Eine Unterscheidung zwischen Schmelz, Dentin und Zement konnte mittels QRA nicht getroffen werden. Sowohl bei den klinischen als auch bei den QRA-Parametern treten signifikante Gruppenunterschiede erstmalig nach sechs Monaten auf. Allerdings wurde bei der QRA ein Trend bereits 14 Tage nach Füllungslegung deutlich. Demzufolge lieferte die QRA trotz der kleineren Kohorte (n = 11) diese Gruppenunterschiede früher als die Bewertung mit klinischen Kriterien (n = 22). Zusammenfassend zeigte die QRA eine höhere Sensitivität und Reliabilität als die Betrachtung mit klinischen Parametern und ermöglichte eine frühzeitigere Aussage zur klinischen Bewährung von Adhäsivsystemen.:Inhaltsverzeichnis Kurzreferat II Abkürzungsverzeichnis III 1. Einführung in die Thematik 1 1.1. Adhäsivsysteme 1 1.2. Bewertungsmethoden zur Untersuchung des Adhäsivverbundes 3 1.3. Quantitative Randanalyse mit dem Rasterelektronenmikroskop 4 2. Zielsetzung und Arbeitshypothesen 9 3. Publikationsmanuskript 10 4. Zusammenfassung 35 5. Literatur IV 6. Darstellung des eigenen Beitrags XI Selbstständigkeitserklärung XIII

O objetivo da presente tese foi verificar a influência do enxágue bucal com carboidrato (CHO) sobre o desempenho no exercício físico predominantemente aeróbio realizado após uma atividade cognitiva frequentemente utilizada para induzir a fadiga mental. Sete homens fisicamente ativos participaram do estudo (idade: 26 ± 5 anos; estatura: 176,0 ± 8,3 cm; massa corporal: 73,6 ± 7,4 kg e gordura corporal: 14,3 ± 3,7 %, VO2 pico: 43,9 ± 6,5 ml.kg-1.min-1, Wpico: 268 ± 23,8 W) realizaram o Stroop Incongruente para indução da Fadiga Mental (FM) ou assistiam a um documentário pelo mesmo período de tempo (controle, CON). Após a tarefa eles realizaram um exercício de carga constante (~55%Wpico) até exaustão. Em cada uma das condições FM e CON os voluntários utilizavam o enxágue bucal (10s) com CHO ou placebo (PLA). No pre-exercício, não houve diferença estatisticamente significante para glicose entre as diferentes condições. A frequência cardíaca apresentou aumento após todas as intervenções, assim como a fadiga percebida (P = 0,001, ES = 0,49), mas sem efeito de condição. O effect size foi moderado para essa variável, sugerindo que o aumento na fadiga percebida foi maior na condição FM (75%) quando comparado a condição CON (60%). A motivação não apresentou diferença entre as condições. Porém, houve uma tendência (0,07) a ser maior na condição DOC. Houve uma redução de 7,8% no tempo até exaustão na condição FM comparado ao CON, entretanto, essa diferença não foi estatisticamente significante. A frequência cardíaca, percepção subjetiva do esforço, fadiga percebida e eletromiografia do músculo vasto lateral aumentaram em função do tempo, sem diferença entre as condições. Com base nesses achados, pode-se concluir que a FM produz apenas um pequeno efeito deletério no exercício físico prologado e que o enxágue com CHO não era capaz de influenciar nessas condições experimentais. Possivelmente, isso se deve ao fato de ambas as intervenções estudadas (FM e enxágue com CHO) produzirem apenas pequenas alterações nas variáveis fisiológicas e perceptivas antes do exercício físico / The aim of this thesis was verify the influence of carbohydrate mouth rinse (CHO) on endurance performance after cognitive activity frequently used to induce mental fatigue. Seven health men (age: 26 ± 5 years; height: 176.0 ± 8.3 cm; weight: 73.6 ± 7.4 kg; and corporal fat: 14.3 ± 3.7%, VO2peak: 43.9 ± 6.5 ml.kg-1.min-1, Wpeak: 268.0 ± 23.8 W) performed an Incongruent Stroop to induce mental fatigue (FM) state or watched a documentary (control, CON) prior to endurance exercise. Following, they performed a workload exercise (~55%Wpeak) until exhaustion with CHO or placebo (PLA) mouth rinse (10s). In the pre-exercise, there was no significant difference for blood glucose between conditions. There was a main effect of distance for heart rate and perceived fatigue, but without interaction (P > 0.05) or condition (P > 0.05) effects. However, there was a moderate effect size for perceived fatigue (ES = 0,49), suggesting a possible relevant increase in perceived fatigue in the FM condition (75%) when compared to CON condition (60%). Although did not reach a significance level, the motivation tended (P = 0.07) to be greater in DOC condition. There was a reduction of 7.8% in time to exhaustion in FM condition compared to CON, however did not reach a significance level (P > 0.05). During exercise, the heart rate, rating perceived exertion, perception of fatigue and electromyography of the vastus lateralis increased over time (P < 0.05), but without interaction (P > 0.05) or condition (P > 0.05) effects. These results suggest that FM produce only a small deleterious effect in the prolonged physical exercise and that carbohydrate mouth rinse was not able to influence the endurance performance at all experimental conditions. Possibly, this is due to the fact of the experimental interventions (FM and carbohydrate mouth rinse) produced only discrete changes in the physiological and perceptual variables before endurance exercise

Made available in DSpace on 2017-07-24T19:22:33Z (GMT). No. of bitstreams: 1 Tese Miguel Munoz Doutorado Odontologia 2014.pdf: 3888005 bytes, checksum: 9a25e76295a44d9cdb11d6afb38c9041 (MD5) Previous issue date: 2014-02-07 / Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná / The aim of the present study was to make an in vitro evaluation of the properties and stability of the bond to dentin of new universal adhesive systems applied according to the etch-and-rinse (ER) and self-etching (SE) adhesive strategies. The specific objectives for each of the three studies were: To evaluate the microtensile bond strength (RU), nanoleakage (NI) and degree of conversion (DC) in the time intervals of 24 h (Experiment 1) and six months (Experiment 2) of the universal adhesive systems applied in accordance with the manufacturers’ recommendations for strategies ER and SE; and the effect of an additional hydrophobic layer (Experiment 3, in the immediate time interval) on these same properties. The occlusal surfaces of 140 third molars were flattened until dentin was obtained, and then the teeth were randomly allocated to 28 groups (n=5). In Experiments 1 and 2, 40 teeth were divided into eight groups according to the adhesive used (Peak Universal [Pk], Scotchbond Universal [Sc], Allbond Universal [Al]) and adhesive strategy (ER and SE), with the ER adhesive system Adper Single Bond 2 (SB) and self-etching Clearfil SE Bond (CSE) used as controls. For Experiment 3, 60 teeth were divided into 12 groups according to the adhesive (Sc, Al, G-Bond Plus [Gbp]), adhesive strategy (ER and SE) and with or without hydrophobic layer (Heliobond, HE). After application of the variables adhesives and restorative procedure, the teeth were stored in distilled water (37oC/24 h) and cut to obtain dentin-resin sticks (0.8 mm2 cross-section) which were submitted to the RU and NI tests in the following time intervals: immediate (Experiments 1 and 3) and six months (Experiment 2); and analysis of DC in the immediate time interval. The data of Experiment 1 were submitted to one-way analysis of variance (Adhesive Strategy) and those of Experiments 2 and 3, to twoway analysis of variance (Adhesive strategy vs. Time intervals; and Adhesive strategy vs. Hydrophobic layer) respectively. The analyses were complemented by the Tukey Test (α=0.05). The RU results obtained in Experiment 1 demonstrated that only PkEr and PkSe were similar to the respective control groups (p>0.05); that the other universal materials resulted in lower RU means than those of their controls ER and SE (p<0.05) and that AlSe presented the lowest mean RU values (p<0.05). For NI, the Groups ScEr, ScSe, AlSe, and AlEr presented low levels of NI similar to those of the control groups (p<0.05). For DC, only Group ScSe demonstrated low DC when compared with the other materials (p<0.05). According to the results of Experiment 2, the systems Pk and AlEr presented a reduction in RU (p<0.05) and only Pk underwent significant degradation (NI) after six months (p<0.05). According to Experiment 3, Al and Gbp obtained higher RU results with the ER strategy (p<0.05); the use of HE increased the RU only in strategy SE (p<0.05); a low level of NI was observed only in Groups Sc and Al with the CON+HE strategy and in Group Gbp with SE+HE; Groups Sc and Gbp presented a high DC when used with the ER strategy, which was increased with the application of HE (p<0.05). The DC if Al was similar under all the conditions. From the three experiments of which this study was composed, it could be concluded that: 1) the universal adhesives presented a 13 material-dependent behavior in both strategies (ER and SE) and were inferior, in at least one of the properties evaluated (RU, Nl or DC), when compared with the control adhesives (CSE and SB); 2) In general, after six months the adhesives SE maintained the most stable RU and NI values, which appears to be related mainly to their capacity to form a chemical bond to dental substrate; 3) The use of a hydrophobic layer in strategy SE was able to increase the RU and DC of universal adhesives and the reduction in NI was more dependent on the composition of the adhesive than on the adhesive strategy. / O objetivo do presente trabalho foi avaliar in vitro as propriedades e longevidade da união à dentina dos novos sistemas adesivos universais aplicados segundo as estratégias adesivas convencional (CON) e autocondicionante (AC). Os objetivos específicos para cada um dos três estudos foram: avaliar a resistência de união à microtração (RU), a nanoinfiltração (NI) e o grau de conversão (GC) no tempo imediato - 24 h - experimento 1) e seis meses (Experimento 2) dos sistemas adesivos universais para as estratégias CON e AC; e o efeito de uma camada hidrofóbica adicional (Experimento 3, no tempo imediato) sobre essas mesmas propriedades. Cento e quarenta terceiros molares foram planificados na face oclusal até se obter dentina e alocados aleatoriamente em 28 grupos (n=5). Nos Experimentos 1 e 2, 40 dentes foram divididos em oito grupos de acordo com o adesivo (Peak Universal [Pk], Scotchbond Universal [Sc], Allbond Universal [Al]) e estratégia adesiva (CON e AC) tendo como controle, o sistema adesivo convencional Adper Single Bond 2 (SB) e o autocondicionante Clearfil SE Bond (CSE). Para o Experimento 3, 60 dentes foram divididos em 12 grupos de acordo com o adesivo (Sc, Al, G-Bond Plus [Gbp]), estratégia adesiva (CON e AC) e com a presença ou não de camada hidrofóbica (Heliobond, HE). Após aplicação das variáveis adesivas e do procedimento restaurador, os dentes foram armazenados em água destilada (37oC/24 h) e cortados para obter palitos de dentina-resina (0,8 mm2 de secção transversal) sendo submetidos aos teste de RU e NI nos tempos imediato (Experimentos 1 e 3) e também seis meses (Experimento 2); e análise de GC no tempo imediato. Os dados do Experimento 1 foram submetidos à análise de variância de um fator (Estratégia Adesiva) e os dos Experimentos 2 e 3, à de dois fatores (Estratégia adesiva versus Tempos de análise; e Estratégia adesiva versus Camada hidrofóbica), respectivamente. As análises foram complementadas pelo teste de Tukey (α=0,05). Os resultados de RU obtidos no Experimento 1 demonstraram que apenas PkCon e PkAc foram similares aos respectivos grupos controle (p>0,05), que os outros materiais universais resultaram em médias de RU inferiores aos seus controles CON e AC (p<0,05) e que AlAc apresentou as médias mais baixas de RU (p<0,05). Para NI, ScCon, ScAc, AlAc, e AlCon apresentaram baixos níveis de NI similares aos grupos controle (p<0,05). Para GC, unicamente ScAc demostrou baixo GC, quando comparado com os outros materiais (p<0.05). Segundo os resultados do Experimento 2, os sistemas Pk e AlCon apresentaram diminuição da RU (p<0,05) e apenas Pk teve degradação significativa (NI) após seis meses (p<0,05). De acordo com o Experimento 3, Al e Gbp obtiveram maiores resultados de RU na estratégia CON (p<0,05); o uso de HE aumentou a RU unicamente na estratégia AC (p<0,05); baixa NI foi observada somente em Sc e Al na estratégia CON+HE e em Gbp em AC+HE; Sc e Gbp apresentaram alto GC quando usados em estratégia CON, que foi aumentado com a aplicação de HE (p<0,05). O GC de Al foi similar em todas as condições. A partir dos três experimentos que compõem o presente trabalho, pôdese concluir que: 1) os adesivos universais apresentaram um comportamento 11 dependente do material em ambas estratégias (CON e AC) e foram inferiores, pelo menos em uma das propriedades avaliadas (RU, Nl ou GC), quando comparados aos adesivos controle (CSE e SB); 2) Em geral, após seis meses os adesivos AC mantém mais estáveis os valores de RU e NI, o que parece estar relacionado principalmente à sua capacidade de união química ao substrato dental; 3) A utilização de uma camada hidrofóbica na estratégia AC pôde aumentar a RU e o GC de adesivos universais e a redução da NI foi mais dependente da composição do adesivo do que da estratégia adesiva.

O objetivo da presente tese foi verificar a influência do enxágue bucal com carboidrato (CHO) sobre o desempenho no exercício físico predominantemente aeróbio realizado após uma atividade cognitiva frequentemente utilizada para induzir a fadiga mental. Sete homens fisicamente ativos participaram do estudo (idade: 26 ± 5 anos; estatura: 176,0 ± 8,3 cm; massa corporal: 73,6 ± 7,4 kg e gordura corporal: 14,3 ± 3,7 %, VO2 pico: 43,9 ± 6,5 ml.kg-1.min-1, Wpico: 268 ± 23,8 W) realizaram o Stroop Incongruente para indução da Fadiga Mental (FM) ou assistiam a um documentário pelo mesmo período de tempo (controle, CON). Após a tarefa eles realizaram um exercício de carga constante (~55%Wpico) até exaustão. Em cada uma das condições FM e CON os voluntários utilizavam o enxágue bucal (10s) com CHO ou placebo (PLA). No pre-exercício, não houve diferença estatisticamente significante para glicose entre as diferentes condições. A frequência cardíaca apresentou aumento após todas as intervenções, assim como a fadiga percebida (P = 0,001, ES = 0,49), mas sem efeito de condição. O effect size foi moderado para essa variável, sugerindo que o aumento na fadiga percebida foi maior na condição FM (75%) quando comparado a condição CON (60%). A motivação não apresentou diferença entre as condições. Porém, houve uma tendência (0,07) a ser maior na condição DOC. Houve uma redução de 7,8% no tempo até exaustão na condição FM comparado ao CON, entretanto, essa diferença não foi estatisticamente significante. A frequência cardíaca, percepção subjetiva do esforço, fadiga percebida e eletromiografia do músculo vasto lateral aumentaram em função do tempo, sem diferença entre as condições. Com base nesses achados, pode-se concluir que a FM produz apenas um pequeno efeito deletério no exercício físico prologado e que o enxágue com CHO não era capaz de influenciar nessas condições experimentais. Possivelmente, isso se deve ao fato de ambas as intervenções estudadas (FM e enxágue com CHO) produzirem apenas pequenas alterações nas variáveis fisiológicas e perceptivas antes do exercício físico / The aim of this thesis was verify the influence of carbohydrate mouth rinse (CHO) on endurance performance after cognitive activity frequently used to induce mental fatigue. Seven health men (age: 26 ± 5 years; height: 176.0 ± 8.3 cm; weight: 73.6 ± 7.4 kg; and corporal fat: 14.3 ± 3.7%, VO2peak: 43.9 ± 6.5 ml.kg-1.min-1, Wpeak: 268.0 ± 23.8 W) performed an Incongruent Stroop to induce mental fatigue (FM) state or watched a documentary (control, CON) prior to endurance exercise. Following, they performed a workload exercise (~55%Wpeak) until exhaustion with CHO or placebo (PLA) mouth rinse (10s). In the pre-exercise, there was no significant difference for blood glucose between conditions. There was a main effect of distance for heart rate and perceived fatigue, but without interaction (P > 0.05) or condition (P > 0.05) effects. However, there was a moderate effect size for perceived fatigue (ES = 0,49), suggesting a possible relevant increase in perceived fatigue in the FM condition (75%) when compared to CON condition (60%). Although did not reach a significance level, the motivation tended (P = 0.07) to be greater in DOC condition. There was a reduction of 7.8% in time to exhaustion in FM condition compared to CON, however did not reach a significance level (P > 0.05). During exercise, the heart rate, rating perceived exertion, perception of fatigue and electromyography of the vastus lateralis increased over time (P < 0.05), but without interaction (P > 0.05) or condition (P > 0.05) effects. These results suggest that FM produce only a small deleterious effect in the prolonged physical exercise and that carbohydrate mouth rinse was not able to influence the endurance performance at all experimental conditions. Possibly, this is due to the fact of the experimental interventions (FM and carbohydrate mouth rinse) produced only discrete changes in the physiological and perceptual variables before endurance exercise

Bakgrund:Oral mukosit (OM) är en toxisk biverkan efter högdos cytostatikabehandling (HDC) och hematopoietisk stemcellstransplantation (HSCT). OM orsakar kliniska komplikationer samt negativa följder för patienten, som längre sjukhusvistelse, oral smärta, viktförlust och parenteral nutrition (PN).Syfte:Att undersöka om det föreligger skillnad i viktförändring och nutritionsstatus hos patienter som använder munsköljmedlet, Caphosol ® i tillägg till standardbehandling i jämförelse med standardbehandling vid behandling med HDC och HSCT.Metod:En randomiserad kontrollerad öppen studie där patienter &gt; 16 år (n=40), behandlades med HDC, inför HSCT på Akademiska universitetssjukhuset, Uppsala. Patienterna randomiserades, 1:1, till oral standardbehandling och munsköljmedlet Caphosol® (EXP n=20) eller oral standardbehandling (KTR n=20). OM, oral smärta, viktförlust och dagar av PN registrerades och analyserades från baseline till 21 dagar efter avslutad HDC.Resultat:Caphosol ® hade ingen signifikant betydelse för viktförändringar mellan EXP- och KTR-grupperna. OM-smärta debuterade senare i EXP än i KTR-gruppen. KTR gruppen använde mer PN jämfört med EXP-gruppen.Konklusion:Caphosol ® hade obetydlig inverkan på förekomst, duration och svårighetsgrad av OM under HCT vid HSCT och därmed liten effekt på nutrition och vikt. Det förelåg ingen fördel att addera Caphosol ® till oral standardbehandling. / Background:Oral mucositis (OM) is a result of cytotoxic effects of high dose chemotherapy (HDCT) administered before hematopoietic stem cell transplantation (HSCT). It is a source of negative consequences for the patient, such as longer hospitalization, oral pain, weight loss, and use of parenteral nutrition (PN).Objective:To investigate whether there is differences in weight changes and nutritional status in patients receiving mouth rinse, Caphosol®, in addition to standard oral care (OC) compared to standard OC for HDCT and HSCT.Method:A randomized, controlled open study with patients &gt; 16 years, treated with HDCT before HSCT at Akademiska University Hospital, Uppsala, Sweden. Patients randomized 1:1 to standard OC and Caphosol® (EXP, n=20) or standard OC (CTR n = 20). Oral pain, weight loss and days of PN was recorded and analysed from baseline to day 21 post HDCT.Result:Caphosol ® had no significant impact on weight changes between EXP and CTR groups. OM-pain peaked later in the EXP group than in CTR. No significance in weight change between settings. CTR group had higher use of PN compared to EXP.Conclusion:Caphosol® had little effect on frequency, duration and severity of OM and thereby little effect on nutrition and weight. There was no advantage to add Caphosol ® to standard OC.

Magister Chirurgiae Dentium - MChD (Oral Medicine and Periodontics) / The aim of this study was to assess whether a mouthwash containing carbohydrate derived fulvic acid, is effective in reducing the salivary microbial count pre-operatively. Endeavours have been made to reduce the risk of infection, bacteraemia and cross-contamination during dental procedures by the application of topical antimicrobial agents. To date chlorhexidine is the most widely evaluated and efficacious agent against oral biofilms but there have been reports of adverse effects ranging from contact dermatitis to severe anaphylactic shock. A new mouth rinse containing carbohydrate derived fulvic acid are reported to have broad spectrum antimicrobial activity against specific oral microbes and Candida albicans with no side effects.

Magister Chirurgiae Dentium (MChD) / Purpose: To evaluate and compare the in-vitro shear bond strength and micro leakage of two compomers with their adhesive systems and to examine the dentine-restorative interface under confocal scanning laser microscopy (CSLM). Matoiats and Methods: For shear bond strength (SBS) testing thirty non-carious human molars were used of which fifteen molars were restored with Dyract AP using Non-Rinse Conditioner (NRC) and Prime&Bond NT (PBNT) and fifteen were restored with F2000 and Scotchbond Multi-Purpose Plus (SBMP). For the microleakage evaluation cavity preparations were made on the facial surfaces of thirty non-carious premolars. These were then restored with the respective compomer system. The specimens were thermocycled, sectioned and examined for dye penetration. The dentine-restorative interface was examined through a confocal scanning laser microscope. The primers of the bonding agents were labelled with rhodamine B and the adhesive resins were labelled with fluorescein and examined under CSLM in fluorescent mode. Results: The mean SBS for PBNT and SBMP were 12.8 and 18.1 MPa, respectively. The microleakage scores showed Dyract with PBNT leaked on the dentine side in 13 of the 15 specimens examined. On the enamel side 2 of the 15 specimens showed microleakage. With F2000 and SBMP no micro leakage was observed on either enamel or dentine sides. The CSLM images show clear resin tag and hybrid layer formation for both the materials examined, although SBMP showed deeper penetration into the dentine with longer resin tags. The length of the resin tags and thickness of the hybrid layer for PBNT was found to be approximately 10 um and 2 um respectively. SBMP showed resin tags measuring about 100 um while the hybrid layer measured about 5 um. Conclusion: This study demonstrates that the acid-etch technique ofSBMP with F2000 produces higher bond strength and no micro leakage when compared to the self-etching/self-priming "non-rinse technique" of NRC with PBNT and Dyract.

No description available.

Detection of p16 through immunohistochemistry (IHC) is the standard for determining the HPV status of the tumor according the TNM eighth edition released in 2017 and has become crucial for determining the HPV status of oropharyngeal squamous cell carcinomas (OPSCC) with direct impact on staging and prognostication. In recent years, detection of HPV DNA in mouthwashes has been proposed as a noninvasive alternative, both for OPSCCs and for other head and neck squamous cell carcinomas (HNSCCs). However, the prospect of using the mouthwashes to monitor the response to therapy is unclear. To evaluate the effect of curative therapy on the detection of HPV DNA, we performed a prospective study comparing the detection frequency of high-risk HPV DNA (HR-HPV-DNA) in pre- and post-therapy mouthwashes. We collected 137 mouthwashes from 88 pathologically confirmed HNSCC patients for DNA isolation and HPV genotyping with the Inno- LiPA assay. We show that HPV DNA in pretherapeutic mouthwashes can detect HPV-driven HNSCCs with a sensitivity of 50.0% and specificity of 85.4%, alongside a high negative predictive value of 79.5% and an accuracy of 74.5%. Furthermore, we observed a notable decrease in the detection frequency of HR-HPV-DNA after successful treatment (pre-therapy 50.0% (9/18) versus post-therapy 9.7% (3/28)). However, the comparatively low sensitivity regarding detection of HPV-driven OPSCC argues against its use in clinical routine.