Global ETD Search

11	The effects of osmotic stress on the structure and function of the cell nucleus. Finan, JD, Guilak, F 15 February 2010 (has links) Osmotic stress is a potent regulator of the normal function of cells that are exposed to osmotically active environments under physiologic or pathologic conditions. The ability of cells to alter gene expression and metabolic activity in response to changes in the osmotic environment provides an additional regulatory mechanism for a diverse array of tissues and organs in the human body. In addition to the activation of various osmotically- or volume-activated ion channels, osmotic stress may also act on the genome via a direct biophysical pathway. Changes in extracellular osmolality alter cell volume, and therefore, the concentration of intracellular macromolecules. In turn, intracellular macromolecule concentration is a key physical parameter affecting the spatial organization and pressurization of the nucleus. Hyper-osmotic stress shrinks the nucleus and causes it to assume a convoluted shape, whereas hypo-osmotic stress swells the nucleus to a size that is limited by stretch of the nuclear lamina and induces a smooth, round shape of the nucleus. These behaviors are consistent with a model of the nucleus as a charged core/shell structure pressurized by uneven partition of macromolecules between the nucleoplasm and the cytoplasm. These osmotically-induced alterations in the internal structure and arrangement of chromatin, as well as potential changes in the nuclear membrane and pores are hypothesized to influence gene transcription and/or nucleocytoplasmic transport. A further understanding of the biophysical and biochemical mechanisms involved in these processes would have important ramifications for a range of fields including differentiation, migration, mechanotransduction, DNA repair, and tumorigenesis. / Dissertation Animals Cell Nucleus Humans Osmolar Concentration Osmosis Osmotic Pressure Signal Transduction
12	Molecular correlates of adaptation and apoptosis : p38 signaling in hippocampus Niswander, Julie Marie. January 2004 (has links) Thesis (Ph.D.)--Medical College of Ohio, 2004. / "In partial fulfillment of the requirements for the degree of Doctor of Philosophy in Medical Sciences." Major advisor: Linda A. Dokas. Document formatted into pages: iv, 150 p. Title from title page of PDF document. Bibliography: pages 44-52.
13	Effets des diètes salées sur l'équilibre hydrominéral et l'acclimatation à l'eau salée de l'omble de fontaine (Salvelinus fontinalis) / Angers, Bernard. January 1992 (has links) Mémoire (M.Sc.B.) -- Université du Québec à Chicoutimi, 1992. / Document électronique également accessible en format PDF. CaQCU
14	Intra-arterial and intravenous applications of Iosimenol 340 injection, a new non-ionic, dimeric, iso-osmolar radiographic contrast medium: phase 2 experience Meurer, Karoline, Laniado, Michael, Hosten, Norbert, Kelsch, Bettina, Hogstrom, Barry 18 September 2019 (has links) Background: Iosimenol 340 injection is a new, dimeric, iso-osmolar, iodinated contrast medium for X-ray angiography. Purpose: To compare the safety and efficacy of iosimenol injection to iodixanol injection in two randomized, controlled phase 2 trials. Material and Methods: One hundred and forty-four adult patients were enrolled in the two trials, one for evaluation during arteriography and the other for evaluation during computed tomography. Safety was compared by assessing adverse events, vital signs, ECGs, and laboratory parameters. Efficacy was assessed as X-ray attenuation in the computed tomography (CT) trial and as the quality of contrast enhancement in the arteriography trial. Results: There were no statistically significant differences in terms of safety or efficacy between the two contrast media. Both were well tolerated upon intravenous as well as intra-arterial injection. The most common adverse event was a feeling of warmth (observed in 35.1% of the patients with Iosimenol injection and 44.3% with iodixanol injection). Conclusion: Iosimenol upon intravenous as well as upon intra-arterial injection exhibits a safety profile and shows an efficacy similar to that of iodixanol. info:eu-repo/classification/ddc/610 ddc:610
15	Application of solid phase microextraction with gas chromatography-mass spectrometry as a rapid, reliable, and safe method for field sampling and analysis of chemical warfare agent precursors / Parrish, Douglas K. January 2005 (has links) (PDF) Thesis (Ph. D.)--Uniformed Services University of the Health Sciences, 2005. / Typescript (photocopy).
16	Chondroitin-based nanoplexes as peptide delivery systems-Investigations into the self-assembly process, solid-state and extended release characteristics Umerska, A., Paluch, Krzysztof J., Santos-Martinez, M.J., Medina, C., Corrigan, O.I., Tajber, L. 20 April 2015 (has links) Yes / A new type of self-assembled polyelectrolyte complex nanocarrier composed of chondroitin (CHON) and protamine (PROT) was designed and the ability of the carriers to bind salmon calcitonin (sCT) was examined. The response of sCT-loaded CHON/PROT NPs to a change in the properties of the liquid medium, e.g. its pH, composition or ionic strength was studied and in vitro peptide release was assessed. The biocompatibility of the NPs was evaluated in Caco-2 cells. CHON/PROT NPs were successfully obtained with properties that were dependent on the concentration of the polyelectrolytes and their mixing ratio. X-ray diffraction determined the amorphous nature of the negatively charged NPs, while those with the positive surface potential were semi-crystalline. sCT was efficiently associated with the nanocarriers (98-100%) and a notably high drug loading (13-38%) was achieved. The particles had negative zeta potential values and were homogenously dispersed with sizes between 60 and 250 nm. CHON/PROT NPs released less than 10% of the total loaded peptide in the first hour of the in vitro release studies. The enthalpy of the decomposition exotherm correlated with the amount of sCT remaining in NPs after the release experiments. The composition of medium and its ionic strength was found to have a considerable influence on the release of sCT from CHON/PROT NPs. Complexation to CHON markedly reduced the toxic effects exerted by PROT and the NPs were compatible and well tolerated by Caco-2 cells. Caco-2 cells: Calcitonin Administration & dosage Metabolism: Cell Survival Pharmaceutical Chondroitin Toxicity: Crystallography X-Ray Delayed-action preparations Drug carriers Humans Hydrogen-ion concentration Kinetics Nanomedicine Nanoparticles Osmolar concentration: Protamines chemistry & metabolism Protein binding Solubility Surface properties Technology Pharmaceutical methods Biocompatibility Peptide delivery Polyelectrolyte complex Protamine toxicity

Page generated in 0.0401 seconds