<p>Diabetes-induced renal complications, i.e. diabetes nephropathy, are a major cause of morbidity and mortality. The exact mechanism mediating the negative influence of hyperglycaemia on renal function is unclear, although several hypotheses have been postulated. Glucose-induced excessive formation of reactive oxygen species (ROS) and increased glucose flux through the polyol pathway are two major mechanisms that have recently gained increasing support. In order to investigate the development of hyperglycaemia-induced renal alterations further, it is of great importance to use an animal model in agreement with the pathological development in diabetic patients.</p><p>The aims of these investigations were to evaluate the streptozotocin (STZ)-diabetic Wistar Furth rat as a model for human diabetic nephropathy and to investigate involvement of ROS and the polyol pathway in development of diabetes-induced renal alterations.</p><p>The used STZ-diabetic animal model displayed several similarities with the progression of human disease, including initial hyperfiltration and albuminuria. However, the observed proteinuria could be partly linked to the STZ treatment <i>per se</i>, making the use of this animal model less suitable for research concerning diabetes-induced urinary protein leakage.</p><p>The diabetic state induced numerous alterations in renal function and metabolism, including increased oxygen consumption, decreased renal oxygen tension (pO₂), and altered lactate/pyruvate ratio. These renal alterations were preventable by daily treatment with either a radical scavenger (α-tocopherol) or an aldose reductase inhibitor (AL-1576).</p><p>In separate experiments the influence of nitric oxide (NO) on renal blood perfusion and pO₂ was investigated. The diabetic animals displayed a larger increase in renal NO activity after injecting the NO substrate L-arginine compared to non-diabetic animals, suggesting substrate limitation of the nitric oxide synthase during chronic hyperglycaemia.</p><p>In conclusion, the results from these investigations show that both ROS and the polyol pathway are involved in the development of diabetes-induced renal alterations in the STZ- diabetic Wistar Furth rat.</p>

Diabetes-induced renal complications, i.e. diabetes nephropathy, are a major cause of morbidity and mortality. The exact mechanism mediating the negative influence of hyperglycaemia on renal function is unclear, although several hypotheses have been postulated. Glucose-induced excessive formation of reactive oxygen species (ROS) and increased glucose flux through the polyol pathway are two major mechanisms that have recently gained increasing support. In order to investigate the development of hyperglycaemia-induced renal alterations further, it is of great importance to use an animal model in agreement with the pathological development in diabetic patients. The aims of these investigations were to evaluate the streptozotocin (STZ)-diabetic Wistar Furth rat as a model for human diabetic nephropathy and to investigate involvement of ROS and the polyol pathway in development of diabetes-induced renal alterations. The used STZ-diabetic animal model displayed several similarities with the progression of human disease, including initial hyperfiltration and albuminuria. However, the observed proteinuria could be partly linked to the STZ treatment per se, making the use of this animal model less suitable for research concerning diabetes-induced urinary protein leakage. The diabetic state induced numerous alterations in renal function and metabolism, including increased oxygen consumption, decreased renal oxygen tension (pO2), and altered lactate/pyruvate ratio. These renal alterations were preventable by daily treatment with either a radical scavenger (α-tocopherol) or an aldose reductase inhibitor (AL-1576). In separate experiments the influence of nitric oxide (NO) on renal blood perfusion and pO2 was investigated. The diabetic animals displayed a larger increase in renal NO activity after injecting the NO substrate L-arginine compared to non-diabetic animals, suggesting substrate limitation of the nitric oxide synthase during chronic hyperglycaemia. In conclusion, the results from these investigations show that both ROS and the polyol pathway are involved in the development of diabetes-induced renal alterations in the STZ- diabetic Wistar Furth rat.

Syfte med denna studie var att undersöka energiförbrukningen vid havspaddling. Energiförbrukningen undersöktes i vila och vid två olika hastigheter (4 och 7 km/h), först med en olastad och sedan med en lastad kajak (20 kg). Dessutom undersöktes hur det passiva motståndet förändrades vid successivt ökad hastigheten. Studien genomfördes i en försöksgrupp med jämn könsfördelning och med varierande ålder och paddlingserfarenhet. I studiens första del uppmättes försökspersonernas hjärtfrekvens i förhållande till syrgasupptag och koldioxidproduktion vid en successivt ökad belastning i en kajakergometer. För varje försöksperson kunde sedan en korrelationskurva beräknas mellan hjärtfrekvens och syrgasupptag. Utifrån den hjärtfrekvens som sedan försökspersonen hade under paddlingen kunde syrgasupptaget beräknas. Vid paddling med en olastad kajak och en hastighet av 4 km/h beräknades syrgasupptaget till 5 ml/kg/min. Vid en hastighet 7 km/h så ökade syrgasupptaget signifikant till 11.5 ml/kg/min (p&lt; 0.05). Vid paddling med en lastad kajak så ökade syrgasupptaget signifikant från 6,5 ml/kg/min vid 4 km/h till 14,5 ml/kg/min (p&lt; 0.05) vid 7 km/h. Om energiförbrukningen beräknas utifrån syrgasupptaget för en person på 75 kg skulle en paddelhastighet på 4 km/h innebära en förbrukning på 458 kJ (107,4 kcal/h) med en olastad kajak och 585 kJ (139.8 kcal/h) med en lastad kajak. Vid 7 km/h var motsvarande värden 1080 kJ (258 kcal/h) (olastad kajak) och 1350 kJ (322 kcal/h) (lastad kajak). Det passiva motståndet ökade exponentiellt med ökad hastighet. / The aim of this study was to examine energy consumption when sea kayaking. The energy consumption was examined at rest and at two different speeds (4 and 7 km/h respectively), first with an empty and then with a packed kayak (20 kg). Moreover, the kayaks’ passive drag in the water was measured in order to examine how this was influenced by the speed through the water. The study was implemented in a group of paddlers with even gender distribution but with varying age and experience. In the first part of the experiment, we measured the participants’ heart frequency, oxygen uptake and carbon dioxide production during gradually increased work load in a kayakergometer. For each test subject correlation curves were constructed from the recorded heart rate and oxygen uptake. Based on the persons heart rate during paddling, oxygen uptake was calculated using the individual regression equation which was calculated in the first part of the experiment. When paddling at 4 km/h with an unpacked kayak, the oxygen uptake was measured to 5 ml/kg/min. At 7 km/h, it increased significantly to 11.5 ml/kg/min (p&lt; 0.05). Paddling with a packed kayak oxygen consumption increased to 6.5 ml/kg/min at 4 km/h and significantly to14.5 ml/kg/min at 7 km/h. If energy consumption is calculated on the basis of oxygen uptake for a person weighing 75 kg, at 4 km/h this corresponds to a consumption of 450 kJ (107.4 kcal/h) when kayaking with an unpacked kayak and 585 kJ (139.8 kcal/h) with a packed ditto. At 7 km/h, energy consumption was calculated to 1080 kJ (258 kcal/h) (empty kayak) and 1350 kJ (322 kcal/h) (packed kayak). The passive drag increased exponentially with increased speed.

Tyrimo objektas. Vidutinių ir ilgų nuotolių bėgikų aerobinis pajėgumas ir kvėpavimo sistemos rodiklių sąryšis. Tyrime dalyvavo 19 vidutinio bei didelio meistriškumo vidutinių (pagrindinis varžybinis nuotolis – nuo 600 m iki 2 km, n =9) ir ilgųjų (3 km arba daugiau, n =10) nuotolių bėgikai. Tiriamiesiems buvo atliktas kvėpavimo sistemos funkcinės būklės nustatymas ramybėje ir aerobinio pajėgumo nustatymas atliekant nepertraukiamą nuosekliai didėjantį krūvį. Tikslas - nustatyti vidutinių ir ilgų nuotolių bėgikų aerobinio pajėgumo ir kvėpavimo sistemos funkcijos ypatumus. Darbo uždaviniai: 1. Palyginti vidutinių ir ligų nuotolių bėgikų aerobinį pajėgumą. 2. Palyginti vidutinių ir ligų nuotolių bėgikų kvėpavimo sistemos funkcinius rodiklius. 3. Įvertinti sąryšį tarp bėgikų aerobinio pajėgumo ir kvėpavimo sistemos funkcijos rodiklių. Išsikėlėme hipotezę, kad kvėpavimo funkcinės galimybės dėl skirtingo intensyvumo treniruotės ir varžybinių krūvių turėtų būti didesnės tarp vidutinių nuotolių bėgikų, o aerobinės ištvermės rodikliai turėtų būti didesni tarp ilgų nuotolių bėgikų. Išvados 1. Absoliutus maksimalus deguonies suvartojimas lyginant vidutinių ir ilgų nuotolių bėgikus nesiskiria, o santykinės šio rodiklio reikšmės reikšmingai didesnės ilgų nuotolių bėgikų grupėje. 2. Ilgų nuotolių bėgikų pirmasis ventiliacinis slenkstis ir jį atitinkantis ŠSD didesni ilgų nuotolių bėgikų grupėje, nors maksimalios ŠSD reikšmės nuosekliai didinamo krūvio metu nesiskiria. 3. Bėgimo... [toliau žr. visą tekstą] / Object of research. Middle distance and long distance runners aerobic capacity and the connection of respiratory system indicators. We researched 19 superior level middle distance (main competition distance from 600m to 2 km, n = 9) and long distance (3 km or more, n = 10) runners. Researched sportsman have undergone respiratory system functional condition test in tranquility state and aerobic capacity test during consecutively heavier physical load. Goal of research. To determine the functional features of middle distance and long distance runners aerobic capacity and respiratory system. Tasks: 1. To compare aerobic capacity between middle distance and long distance runners. 2. To compare respiratory system functional indicators between middle distance and long distance runners. 3. Analyze and connection between runners aerobic capacity and respiratory system indicators. Hypothesis. Because of different training intensiveness and competition loads, functional capacities of respiration system are greater of the middle distance runners, and aerobic capacity indicators are greater of the long distance runners. Conclusion. 1. Absolute maximum oxygen consumption is almost the same of both middle distance and long distance runners, while relative indicators are significantly greater in the long distance runners group 2. Long distance runners first ventilator threshold and its corresponding heart contraction rate is greater in the long distance runners group, while maximum heart... [to full text]

SUMMARY THE RELATIONSHIP BETWEEN PUPILS' BODY COMPOSITION, PHYSICAL ACTIVITY AND RECOVERY SPEED AFTER PHYSICAL EXERCISE Hypothesis. We thought that pupils’ who have higher physical activity and normal body weight also have faster recovery after physical exercise. Objectives of the study. To identify the relationship between pupil’s body composition, physical activity and recovery rate after physical load. The aim of the study: 1. To identify the relationship between physical activity and body composition. 2. To identify the relationship between body composition and recovery rate after physical activity. 3. To identify the relationship between physical activity and recovery speed after physical exercise. Subjects. It was made of a group of 40 healthy (20 girls and 20 boys), 10-12 form pupils, chosen by chance, from one Kaunas secondary school. Methods: According to the questionnaire, applying the indirect test technique of maximum oxygen consumption (VO2max), suggested by Huston university. Was estimated pupils physical activity and VO2max. According to the data of the questionnaire, the analysed have been divided into 3 groups of physical activity (PA): low PA; medium PA; high PA. The body composition of the investigated has been measured according bioelectrical impedance method. The following indicators have been measured: body weight, body weight index (BWI); relative fat mass (%); fat free mass (kg), total water amount (kg). To establish functional capacity of... [to full text]

This study combines noninvasive hybrid diffuse optical spectroscopies [near-infrared spectroscopy (NIRS) and diffuse correlation spectroscopy (DCS)] with occlusive calibration for continuous measurement of absolute blood flow (BF), tissue blood oxygenation (StO2), and oxygen consumption rate (VO2) in exercising skeletal muscle. Subjects performed rhythmic dynamic handgrip exercise, while an optical probe connected to a hybrid NIRS/DCS flow-oximeter directly monitored oxy-, deoxy-, and total hemoglobin concentrations ([HbO2], [Hb], and [tHb]), StO2, relative BF (rBF), and relative VO2 (rVO2) in the forearm flexor muscles. Absolute baseline BF and VO2 were obtained through venous and arterial occlusions, respectively, and used to calibrate continuous relative parameters. Previously known problems with muscle fiber motion artifact in optical measurements were mitigated with a novel dynamometer-based gating algorithm. Nine healthy young subjects were measured and results validated against previous literature findings. Ten older subjects with fibromyalgia and thirteen age-matched healthy controls were then successfully measured to observe differences in hemodynamic and metabolic response to exercise. This study demonstrates a novel application of NIRS/DCS technology to simultaneously evaluate quantitative hemodynamic and metabolic parameters in exercising skeletal muscle. This method has broad application to research and clinical assessment of disease (e.g. peripheral vascular disease, fibromyalgia), treatment evaluation, and sports medicine.

This research investigates various applications of diffuse correlation spectroscopy (DCS) on in-vivo human muscle tissue, both at rest and during dynamic exercise. Previously suspected muscle tissue relative blood flow (rBF) baseline shift during extended measurement with DCS and DCS-Near infrared spectroscopy (NIRS) hybrid optical systems are verified, quantified, and resolved by redesign of optical probe and alteration in optical probe attachment methodology during 40 minute supine bed rest baseline measurements. We then translate previously developed occlusion techniques, whereby rBF and relative oxygen consumption rV̇O2 are calibrated to initial resting absolute values by use of a venous occlusion (VO) and arterial occlusion (AO) protocol, respectively, to the lower leg (gastrocnemius) and these blood flows are cross validated at rest by strain gauge venous plethysmography (SGVP). Methods used to continuously observe 0.5Hz, 30% maximum voluntary isometric contraction (MVIC) plantar flexion exercise via dynamometer are adapted for our hybrid DCS-Imagent diffuse optical flow-oximeter in the medial gastrocnemius. We obtain healthy control muscle tissue hemodynamic profiles for key parameters BF, V̇O2, oxygen saturation (StO2), deoxyhemoglobin, oxyhemoglobin, and total hemoglobin concentrations ([Hb], [HbO2], and THC respectively), as well as systemic mean arterial pressure (MAP) and pulse rate (PR), at rest, during VO/AO, during dynamic exercise and during 15 minute recovery periods. Next, we began investigation of muscle tissue hemodynamic disease states by performing a feasibility pilot study using limited numbers of controls and peripheral arterial disease (PAD) patients using the translated methods/techniques to determine the ability of our technology to assess differences in these populations.

Diving vertebrates are constrained by an aquatic existence because they carry a limited store of oxygen whilst submerged and must return to the surface to breathe. Therefore, oxygen stores must be used efficiently in order to maximise aerobic dive duration. The ability to use additional, non-pulmonary gas exchange to obtain oxygen from the water provides a mechanism to alleviate the use of body oxygen stores and extend dive duration. The partitioning of gas exchange between aerial and aquatic modes is predicted by the theory of optimal breathing. As the cost of obtaining oxygen by one mode increases, the reliance on this mode should decrease whilst the reliance on the alternate mode should increase. A number of environmental, physiological and ecological factors can affect metabolic rate and/or the partitioning of gas exchange between aerial and aquatic modes, with consequences for dive duration. The overall aim of this study was to examine the effects of temperature, specific dynamic action and predation on respiratory partitioning and diving behaviour in the Arafura filesnake, Acrochordus arafurae. Filesnakes provide an excellent model to address the aims of this study because they are fully-aquatic and respire bimodally, utilising both aerial and aquatic (cutaneous) gas exchange. Metabolic rate and diving behaviour are usually thermally dependent in diving ectotherms. Given this knowledge, we tested whether A. arafurae was capable of up-regulating cutaneous oxygen uptake to compensate for the temperature induced increases in metabolic rate. Metabolic rate had a Q10(20-32°C) of 2.33, however cutaneous oxygen uptake was independent of temperature and all elevated metabolic demands were met by increasing aerial oxygen uptake. Consequently, maximum dive duration was reduced by 70%, from 77 min at 20°C to 28 min at 32°C. The temperature independence of cutaneous oxygen uptake suggests that blood PO2 remained stable across temperatures and the increased blood flow expected with an elevated metabolic rate did not enhance the capacity for oxygen uptake (diffusion limited rather than perfusion limited). Although cutaneous oxygen uptake was not regulated, its contribution to extending dive duration was significant. When the ability to respire aquatically was removed (severe hypoxia), dive duration was reduced by up to 30%. Acrochordus arafurae feeds infrequently on large, high-protein meals thus the postprandial metabolic response was significant. Meal size and fast length (13 days or one month) were both positively related to the peak metabolic scope. After fasting for one month, peak rate of oxygen consumption was up to 12-fold standard metabolic rate (SMR) with the largest meal size. By comparison, an animal that was fasted for 13 days and fed an equivalent meal size incurred a six-fold increase in SMR. The energetic costs of digestion (specific dynamic action, SDA) increased with meal size, but fast length had no effect, suggesting the up-regulation of gut function was not a significant cost in the SDA. Pulmonary and cutaneous gas exchange increased with elevated metabolic demands however pulmonary oxygen uptake was the dominant mode. Despite the increase in cutaneous oxygen uptake during digestion, maximum dive duration decreased by between 50% and 90% of fasted values. The significant reductions in dive duration following feeding may have implications for predator vulnerability. Diving animals are most vulnerable to predation at the water’s surface. The theory of optimal diving under predation predicts that animals should make shorter, more frequent surface intervals or longer, less frequent surfacing intervals to minimise exposure to predators. Acrochordus arafurae is prone to aerial (birds of prey) and aquatic (crocodiles, large fish) predation. Simulated avian predation did not change dive or surface duration or proportion of time at the surface or spent active. However, a greater number of longer dives were observed with fewer long surface intervals suggesting an increase in the use of cutaneous oxygen uptake. The nocturnal diving patterns of A. arafurae provide an in-built anti-predator strategy. The threat of aquatic predation by a large fish produced atypical anti-predator responses; A. arafurae became increasingly active, reduced dive duration and increased surface interval which was indicative of a foraging response. The field diving behaviour of adult A. arafurae was determined using acoustic telemetry and interpreted in the context of environmental and ecological conditions. The mean dive duration was 6.6 min, with 85% of dives less than 10 min in duration. The maximum dive duration was 153 min. There was no diel pattern in dive duration. Snakes were located at an mean depth of 0.62 m, however, they occasionally ventured to greater depths (up to 6 m) but very infrequently. Snake body temperature reflected water temperature and fluctuated by approximately 1°C on a daily basis. The short and shallow dives of A. arafurae minimise travel costs associated with surfacing and may be an anti-predator strategy. Metabolic rate and respiratory partitioning is dependent on physiological and ecological factors with consequences for dive duration. Temperature and postprandial induced increases in metabolic demands were met by increasing the reliance on aerial gas exchange given the limitations of cutaneous gas exchange; and dive duration was substantially reduced as a result. Ecological factors, such as predation, can increase the cost of surfacing and promote the use of aquatic gas exchange. However, changes to behaviour, including habitat choice, residence depth and the employment of a diving strategy that does not maximise dive duration may be just as effective at minimising the costs and maximising the gains of a dive.

Aims of this thesis were to assesed the impact of feeding, temperature of water and fish size on ammonia excretion and oxygen consumption in Clarias gariepinus in recirculating system. Fish were divided in 8 weight categories from 21 to 2495 g. Experiments were performed under four temperatures: 22°C, 25°C, 28°C and 30°C. Fish were acclimatized, light conditions were: 14 hours of light period and 10 hours of dark period. Feeding was provided fed four times a day. Starved fish were measured. Measurements were held every two hours. Oxygen consumption was measured by multimeter and ammonia excretion using Nessler?s method. Average daily oxygen consumption in fed fish was dependent on temperature and average individual weight of fish, varied from 10.5 to 96.6 mg O2/kg/h and in hungry fish from 4.3 to 61.8 mg O2/kg/h. TAN excretion varied from 0 to 59.9 mg TAN/kg/h in fed fish and from 0 to 60.8 mg TAN/kg/h in hungry fish. Oxygen consumption increased with increasing temperature. Ammonia excretion was very unstable at all temperatures in all weight categories. Fish had higher ammonia excretion in light period of experiment.

O orientador do trabalho n?o mencionado na lista da folha de aprova??o. / Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-08-06T18:31:52Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) andreza_leticia_gomes.pdf: 1090108 bytes, checksum: 96eed10c887fbcff28a65cbd64874639 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-10-05T19:12:17Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) andreza_leticia_gomes.pdf: 1090108 bytes, checksum: 96eed10c887fbcff28a65cbd64874639 (MD5) / Made available in DSpace on 2018-10-05T19:12:17Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) andreza_leticia_gomes.pdf: 1090108 bytes, checksum: 96eed10c887fbcff28a65cbd64874639 (MD5) Previous issue date: 2018 / O Incremental Shuttle Walking Test (ISWT) vem sendo utilizado na avalia??o da aptid?o cardiorrespirat?ria (ACR) de crian?as e adolescentes com diferentes condi??es de sa?de. N?o se sabe se a resposta cardiorrespirat?ria apresentada por adolescentes saud?veis no ISWT ir? se assemelhar aquela induzida pelo teste de esfor?o cardiopulmonar (TECP). Os objetivos deste estudo foram: (1) Avaliar se o ISWT ? um teste m?ximo para adolescentes assintom?ticos do sexo masculino. (2) Propor uma equa??o matem?tica para predizer o pico do consumo de oxig?nio (VO2 pico) e, (3) testar a confiabilidade dessa equa??o para essa popula??o. M?todos: No primeiro est?gio do estudo, 26 participantes realizaram o ISWT e o TECP. No segundo est?gio 50 participantes realizaram o ISWT duas vezes. Em ambos os est?gios foram avaliados VO2 pico, a frequ?ncia card?aca m?xima (FC m?x.) e o pico da raz?o de troca respirat?ria (R pico). No terceiro est?gio foram comparados os valores do VO2 pico preditos pela equa??o criada e obtidos de forma direta no ISWT. Resultados: N?o houve diferen?a significativa no VO2 pico, R pico e FC m?x. obtidos no ISWT e TECP. Os valores encontrados para o VO2 pico (r = 0,44. p = 0,002) e R pico (r = -0,53, p< 0,01) obtidos no ISWT e TECP apresentaram correla??o moderada e significativa, al?m de concord?ncia na an?lise de Bland-Altman. A velocidade da marcha foi a vari?vel que explicou 48% (R2 = 0,48, p = 0,000) da varia??o no VO2 pico no ISWT. Foi criada a equa??o VO2 previsto = 5,490 + (17,093 x Velocidade da Marcha). Os resultados obtidos pela equa??o foram comparados com os valores obtidos pelo analisador de gases e nenhuma diferen?a significativa foi encontrada entre eles. Conclus?es: Em crian?as e adolescentes do sexo masculino o ISWT ? um teste de esfor?o m?ximo com repercuss?es cardiorrespirat?rias similares ao TECP. A equa??o preditiva proposta ? uma estimativa vi?vel para predi??o do VO2 pico para essa popula??o. / Disserta??o (Mestrado Profissional) ? Programa de P?s-Gradua??o em Reabilita??o e Desempenho Funcional, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The Incremental Shuttle Walking Test (ISWT) has been used to evaluate the Cardiorespiratory Fitness (CRF) of children and adolescents with different pathological conditions. It is unknown whether the cardiorespiratory response presented by asymptomatic adolescents in ISWT will be similar to that induced by CardiopulmonaryExerciseStress Test (CEPT). The aims of this study were: (1) To evaluate whether ISWT is a maximum test for asymptomatic male adolescents. (2) To propose a mathematical equation to predict peak oxygen consumption (VO2 peak) and (3) Totest the reliability of this equation on this population. Methods: In the first stage of the study, 26 participants performed the ISWT and the CEPT. In the second stage 33 participants performed the ISWT twice. In both stages, peak VO2, maximal heart rate (HR max) and peak respiratory rate (peak R) were evaluated. In the third stage, the peak VO2 values predicted by the equation created and obtained directly in the ISWT were compared. Results: There was no significant difference in the peak VO2 (p> 0.05), peak R (p> 0.05) and maximum HR obtained in ISWT and CEPT. The values found for the VO2 peak (r = 0.44, p = 0.002) and peak R (r = -0.53, p <0.01) obtained in the ISWT and CEPT presented a moderate correlation and anagreement in the analysis of Bland-Altman analysis. The gait speed was the variable that explained 48% (R2 = 0.48, p = 0.000) of the variation in the peak VO2. The VO2 peak equation = 5.490 + (17.093 x Gait Speed) was created. The results obtained by the equation were compared with the values obtained by the gas analyzer and no significant differences were found between them (p> 0.05). Conclusion: ISWT produced a cardiorespiratory response comparable to CEPT in asymptomatic male adolescents, and the developed equation presented viability to predict peak VO2 in adolescents.