Call number: LD2668 .T4 1984 N39 / Master of Science

O trabalho objetivou construir um dendograma filogenético das cepas de Aspergillus flavus isoladas de amendoim recém-colhido de quatro regiões de São Paulo (Cafelândia, Jaboticabal, Rosália e Tupã), avaliar o potencial toxigênico e agrupar as cepas quanto à produção de esclerócios. A técnica de AFLP foi utilizada para caracterização genotípica. O potencial aflatoxigênico foi avaliado pelo cultivo das cepas em meio de ágar coco, extração das aflatoxinas por clorofórmio, separação por CCD e quantificação por espectrodenditômetro CS-9000. A indução da produção de esclerócios foi feita pela incubação dos isolados em meio ágar Czapeck-DOX. AFLP gerou 78 fragmentos de 27 pb a 365 pb, sendo 13% não polimórficos. O perfil genotípico revelou 31 haplótipos e de 12 grupos no dendograma. A similaridade entre os isolados variou de 37 a 90 %. O potencial aflatoxigênico revelou 91,7 % de cepas produtoras, com níveis entre 39,27 mg/Kg e 28689,61 mg/Kg para AFB1 e 1,50 mg/Kg a 9781,09 mg/Kg para AFB2. Quanto aos esclerócios, 83,9% das cepas foram produtoras, sendo todas tipo S. / This study aimed to draw a phylogenetic dendogram of Aspergillus flavus strains isolated from fresh harvested peanut from four regions of São Paulo state (Cafelândia, Jaboticabal, Rosália and Tupã), to determine the toxigenic potential and to group them regarding the sclerotia production pattern. The AFLP thecnique was used for genotypic characterization. Aflatoxin production was evaluated by inoculation of fungi in coconut agar, extraction with chloroform, TLC segregation and quantification by spectrophotometer CS-9000. Agar Czapeck-DOX was used to evaluate sclerotia production. AFLP generated 78 fragments varying from 27 pb to 365 pb, 13 % of them were not polymorphic. The genotypic profile showed 31 haplotypes and 12 groups in the dendogram. The similarity among the isolates varied from 37 to 90 %. The aflatoxigenic potential showed 91,7 % of producer strains, with levels between 39,27 mg/Kg and 28689,61 mg/Kg for AFB1 and between 1,50 mg/Kg and 9781,09 mg/Kg for AFB2. Concerning the sclerotia production, 83,9 % of the strains were producers, all were S type.

Peanut allergy typically results from sensitisation to one or more integral seed storage proteins; Ara h 1, 3 or 2/6. Reactions can be triggered by as little as 3 mg protein in 10% of allergic individuals and are often severe, inducing anaphylaxis which can be fatal. Accidental exposure through unintended presence can therefore be hazardous and foods must be labelled appropriately. Thermal processing is one of the main factors affecting protein properties in food systems, including the formation of Maillard reaction products. Research has suggested a link between the allergenicity of foods and cooking methods employed. A systematic study was undertaken to assess the thermal dependence of these hazard proteins, focusing on changes to solubility and chemical modifications which may alter IgE binding. Proteomic profiling was used to assess the allergenic content of peanut products and develop alternative methods for allergen analysis to support evidence-based application of precautionary allergen labelling. Runner variety peanuts were processed (boiled, fried, roasted) and their protein content determined. Proteins extracts were characterised by 1D- and 2D-polyacrylamide gel electrophoresis, including differential in-gel electrophoresis. Proteomic profiling was undertaken using label-free analysis to assess allergen composition and investigate the formation of Maillard reaction products on the most clinically relevant proteins. Peanut allergen peptide targets were then identified and used to develop label-based quantitation methods and applied to (i) investigate effects of processing on peptide targets and (ii) determine peanut in chocolate products in comparison with a commercial ELISA kit. Orthogonal studies were performed using serum samples from peanut allergic patients obtained from the Manchester Respiratory, Allergy and Thoracic Surgery (ManARTS) Biobank. Patient IgE reactivity to peanut and processed peanut products was assessed by immunoblotting, inhibition ELISA and mediator release assays. Protein solubility was reduced by thermal processing and processed protein required harsh denaturing conditions for extraction. Qualitative analysis highlighted decreased solubility of key allergens, modifications and aggregation after heating. Proteomic profiling identified and quantified different isoforms of the major peanut allergens. The protein content of processed peanuts was reduced by boiling, specifically the 2S albumins, which transferred into the cooking water. The performance of peptides selected for targeted MRM experiments was influenced by thermal processing and the presence of cocoa phenolics. Ara h 2 peptides flanked by arginine were thermostable and may prove more reliable for quantification. Application of microfluidic separation enhanced the efficiency of target ionisation in complex matrices acquiring important sensitivity gains. Maillard modifications to clinically relevant proteins Ara h 2/6 were found within IgE binding domains in raw and processed peanuts. IgE reactivity studies confirmed reduced IgE binding capacity of extensively boiled peanut and hydrolysed protein, but this did not correlate to a reduction in mediator release in poly-sensitised patients. While effective extraction limits the efficacy of analyses, buffers used in MS analyses are more robust in analysing processed protein. Proteomic profiling provides a means of characterising and profiling of allergenic proteins including food ingredients used in clinical applications. Peptides selected for targeted analyses should be validated to assess their suitability in model foods. Cooking waters collected from extensively boiled peanuts may provide an alternative and safer immunotherapy agent for patients predominantly sensitised to Ara h 2/6.

Peanut (Arachis hypogaea L.) has the potential to become a major source of biodiesel but for market viability, peanut oil yields must increase and specific quality requirements must be met. Oil yield in peanut is influenced by many components, including oil concentration, seed mass, and mean oil produced per seed. All of these traits can be improved through selection as long as there is sufficient genetic variation. Thus, elucidating the genetics of oil concentration, seed mass, and mean oil produced per seed in peanut is essential to advancing the development of genotypes with high oil yields. Additive genetic effects were predominant for oil concentration in two generation means analyses involving a proprietary high oil breeding line and additive genetic variance was highly significant in a complete four-parent diallel analysis. Genetic variance for weight of 50 sound mature kernels (50 SMK) and mean oil produced per SMK (OPS) was additive the diallel analysis. Narrow-sense heritability estimates were high for oil concentration in both the diallel and generation means analyses. Narrow-sense heritability was also high for 50 SMK, but was low for OPS. The low OPS heritability estimate was caused by the negative correlation between oil concentration and seed mass. Consequently, oil concentration and seed mass can be improved through early-generation selection, but large segregating populations from high oil crosses will be needed to identify progeny with elevated oil concentrations that maintain acceptable seed sizes. Increasing the ratio of oleic to linoleic acid (O/L) in peanut oil and reducing the long chain saturated fatty acid concentration (which includes arachidic, behenic, and lignoceric acids) produces high quality, stable methyl esters for biodiesel. Therefore, elucidating the inheritance of these factors and their relationships in peanut populations segregating for high oil is critical. The results from generation means analysis confirm that the high-oleic trait is under simple genetic control and can be manipulated through selection. Oil concentration was negatively correlated with oleic acid concentration in the F2 generations of both crosses and positively correlated with arachidic acid in most of the segregating generations that were evaluated. Therefore, developing a peanut genotype high in oil and oleic acid concentration that has reduced long chain saturates will require the evaluation of large numbers of segregating progeny.

Made available in DSpace on 2014-06-11T19:32:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-02-13Bitstream added on 2014-06-13T18:44:28Z : No. of bitstreams: 1 scaquitto_dc_dr_jabo.pdf: 429936 bytes, checksum: 3e636e555a2a2cd56aa2c19a9d5c4352 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O amendoim (Arachis hypogaea L.) faz parte da família Fabaceae, que é capaz de associar-se com bactérias do gênero Rhizobium. Em solos onde o amendoim é cultivado tem-se a presença de uma população autóctone desta bactéria. A necessidade de inoculação das sementes de amendoim em solos contendo população autóctone tem sido sempre discutida. Com o objetivo de avaliar o efeito da inoculação de sementes de amendoim cultivado em vasos contendo vermiculita e a interação existente entre microrganismo-planta e microrganismo-microrganismo, foi realizado um experimento sob cultivo em casa de vegetação, em vasos de 0,5 L, com o cultivar IAC 886 e a IAC Tatu-ST, com três repetições. Os tratamentos foram constituídos de aplicação dos isolados individualmente e combinados com a estirpe padrão tipo SEMIA 6144, Bradyrhizoibium sp., recomendada pelo Ministério da Agricultura e sem aplicação de inoculante, sem aplicação de qualquer isolado. Houve interação entre o tipo de plantio e a aplicação de inoculante para todas as variáveis analisadas. Para a massa de matéria seca da parte aérea houve uma diferença significativa. O variável número de nódulos e o teor de Nitrogênio também apresentam diferenças quanto à aplicação, sendo que, em todos os casos foram superiores ao controle sem aplicação com os isolados nativos. / Peanut (Arachis hypogaea L.) belonging to Fabacea family, is able of associating with bacteria from Rhizobium genus. In soils where peanut is grown, there is usually an autochthonous population of these bacterial, however, the need of inoculation in peanut seeds in soils containing autochthonous population has always been controversial subject. With the objective of evaluating the effects of peanut seeds inoculation cultivated in vases contend vermiculite and to verifying the interaction with plant regulators, an experiment was carried out in greenhouse conditions, with three repetitions containing Runner IAC 886 e IAC Tatu-ST cultivar. The treatments were constituted of application of the individually isolated and combined with the lineage standard Bradyrhizobium sp., SEMIA 6144, recommended by Bureau of the Agriculture. An interaction was observed between the type of plantation and the application of inoculants for all analysed variable. For the mass of dry matter of the aerial part it had a significant difference. The variable numbers of nodules and rate of Nitrogen also present differences related to the treatments, being that, in all the cases was greater to the control without application of the isolated natives.

abstract: Since the Convention on Biological Diversity was established in 1992, more importance has been given to the conservation of genetic resources in the international community. In 2001, the International Treaty on Plant Genetic Resources for Food and Agriculture (PGRFA) focused on conserving plant genetic resources, including crop wild relatives (CWR). Some of these genetic resources hold desirable traits--such as transfer of plant disease resistance, improvement of nutritional content, or increased resistance to climate change--that can improve commercial crops. For many years, ex situex situ conservation was the prevalent form of protecting plant genetic resources. However, after PGRFA was published in 1998, in situ techniques have increasingly been applied to conserve wild relatives and enhance domesticated crops.In situ techniques are preferred when possible, since they allow for continued evolution of traits through natural selection, and viability of seed stock through continuous germination and regeneration. In my research, I identified regions in Bolivia and rated them according to their potential for successful programs of iin situ conservation of wild crop relatives. In particular, I analyzed areas according to the following criteria: a) The prevalence of CWRs. b) The impacts of climate change, land use change, population growth, and economic development on the continued viability of CWRs in an area. c) The socio-political and economic conditions that might impede or facilitate successful conservation programs and outcomes. This work focuses on three genera of particular importance in Bolivia: Peanut (Arachis spp.), Potato (Solanum spp.) and Quinoa (Chenopodium spp.). I analyzed the above factors for each municipality in Bolivia (the smallest scale for which appropriate data were available). The results indicate which municipalities are most likely to successfully engage in CWR conservation projects. Finally, I present guidelines for the creation of conservation projects that pinpoint some of the potential risks and difficulties with in situ conservation programs in Bolivia and more generally. / Dissertation/Thesis / M.S. Biology 2011

Orientadora: Eliana Gertrudes Macedo Lemos / Banca: Miguel Luiz Menezes Freitas / Banca: Haroldo Alves Pereira Junior / Banca: Jackson Antonio Marcondes de Souza / Banca: Cristina Lacerda Soares Petrarolha Silva / Resumo: O amendoim (Arachis hypogaea L.) faz parte da família Fabaceae, que é capaz de associar-se com bactérias do gênero Rhizobium. Em solos onde o amendoim é cultivado tem-se a presença de uma população autóctone desta bactéria. A necessidade de inoculação das sementes de amendoim em solos contendo população autóctone tem sido sempre discutida. Com o objetivo de avaliar o efeito da inoculação de sementes de amendoim cultivado em vasos contendo vermiculita e a interação existente entre microrganismo-planta e microrganismo-microrganismo, foi realizado um experimento sob cultivo em casa de vegetação, em vasos de 0,5 L, com o cultivar IAC 886 e a IAC Tatu-ST, com três repetições. Os tratamentos foram constituídos de aplicação dos isolados individualmente e combinados com a estirpe padrão tipo SEMIA 6144, Bradyrhizoibium sp., recomendada pelo Ministério da Agricultura e sem aplicação de inoculante, sem aplicação de qualquer isolado. Houve interação entre o tipo de plantio e a aplicação de inoculante para todas as variáveis analisadas. Para a massa de matéria seca da parte aérea houve uma diferença significativa. O variável número de nódulos e o teor de Nitrogênio também apresentam diferenças quanto à aplicação, sendo que, em todos os casos foram superiores ao controle sem aplicação com os isolados nativos. / Abstract: Peanut (Arachis hypogaea L.) belonging to Fabacea family, is able of associating with bacteria from Rhizobium genus. In soils where peanut is grown, there is usually an autochthonous population of these bacterial, however, the need of inoculation in peanut seeds in soils containing autochthonous population has always been controversial subject. With the objective of evaluating the effects of peanut seeds inoculation cultivated in vases contend vermiculite and to verifying the interaction with plant regulators, an experiment was carried out in greenhouse conditions, with three repetitions containing Runner IAC 886 e IAC Tatu-ST cultivar. The treatments were constituted of application of the individually isolated and combined with the lineage standard Bradyrhizobium sp., SEMIA 6144, recommended by Bureau of the Agriculture. An interaction was observed between the type of plantation and the application of inoculants for all analysed variable. For the mass of dry matter of the aerial part it had a significant difference. The variable numbers of nodules and rate of Nitrogen also present differences related to the treatments, being that, in all the cases was greater to the control without application of the isolated natives. / Doutor

Made available in DSpace on 2014-06-11T19:28:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-11-01Bitstream added on 2014-06-13T19:16:24Z : No. of bitstreams: 1 santos_ep_me_jabo.pdf: 811104 bytes, checksum: bcd035d537d9aeb8084fc66fc53510f8 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Copercana / O arranquio, momento de grande importância na produção do amendoim, que se realizada de forma ineficiente pode representar em alguns casos perdas de até 30%. Considerando-se o curto período de tempo disponível para o cultivo do amendoim em áreas de reforma de canaviais, este trabalho teve por objetivo testar a hipótese de que é possível antecipar o arranquio mecanizado do amendoim, sem acarretar prejuízos ao produtor. Para tanto, realizou-se o arranquio do amendoim aos 120, 125, 130, 135 e 140 dias após a semeadura, sendo os resultados avaliados por análise estatística descritiva e multivariada, com uso de análise de variância, para as variáveis: porcentagem de maturação, teor de água das vagens e do solo, índice de cone, perdas no arranquio mecanizado e produtividade. A análise de variância indicou que o adiamento do arranquio de amendoim IAC runner 886 não está diretamente relacionado com o aumento da produtividade, e que as perdas no arranquio tendem a aumentar gradativamente com o aumento do tempo de permanência da cultura no campo, sendo que aos 135 dias após a semeadura, ocorre aumento expressivo destas perdas. A análise multivariada possibilitou analisar de forma conjunta a ação das variáveis, indicando que existe correlação inversa do índice de cone com a produtividade, e entre o teor de água do solo e das vagens com a maturação, além de evidenciar a correlação entre as perdas visíveis e invisíveis do arranquio / In the digging, moment of great importance in the production of peanuts, is conducted in an inefficient way, in some cases may represent losses of up to 30%. Considering the short time available for the cultivation of peanuts in sugarcane areas of reform, this study aimed to test the hypothesis that it is possible to anticipate the mechanical digging from peanuts without causing damage to the producer. Thus, it was carried out the digging the peanut at 120, 125, 130, 135 and 140 days after sowing, and the results were evaluated by multivariate and descriptive statistics analysis, using analysis of variance for the variables: percentage of maturity, water content of the pod and the soil, cone index, losses on the pull-off mechanized and productivity. Analysis of variance indicated that the postponement of the pull-off of the peanut IAC runner 886 is not directly related to increased productivity, and pull-off losses tend to increase gradually with the increase of residence time of the culture in the field, and that 135 days after sowing, there is significant increase in these losses. Multivariate analysis allowed to analyze jointly the action of the variables, indicating that there is an inverse correlation of cone index with productivity, and between the water content of soil and the pods with the maturation, and also highlights the correlation between visible and invisible losses of the digging

Made available in DSpace on 2014-06-11T19:28:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-02-23Bitstream added on 2014-06-13T19:16:30Z : No. of bitstreams: 1 dias_tcs_me_jabo.pdf: 297422 bytes, checksum: a07a72201f2f3cdf8b2d3c758bc91c5b (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A presente pesquisa objetivou avaliar o efeito da redução do espaçamento entrelinhas sobre os períodos de interferência e a produtividade do amendoim rasteiro (Arachis hypogaea cv. Runner IAC 886). O experimento foi instalado no município de Jaboticabal, SP, em um Latossolo Vermelho de textura média. Os tratamentos constaram de dois espaçamentos entrelinhas de semeadura (80 e 90 cm), divididos em dois grupos. No primeiro as plantas daninhas foram controladas desde a emergência até os 0 (inteiramente no mato), 30, 45, 60, 82, 97 e 112 dias. Após estes períodos, as plantas daninhas que germinaram foram deixadas crescer livremente. No segundo, as plantas daninhas conviveram com a cultura pelos mesmos períodos do grupo anterior, sendo que após cada período foram removidas das parcelas. O delineamento experimental foi o de parcelas subdivididas, nas quais se constituíram por parcelas os diferentes espaçamentos e por subparcelas os períodos, totalizando 28 tratamentos em quatro repetições. As principais plantas daninhas presentes na área foram Digitaria sp., Xanthium strumarium, Acanthospermum hispidum e Cenchrus echinatus. Para uma perda tolerável de 5% de produtividade, o período crítico de prevenção da interferência foi dos 27 aos 76 e dos 35 aos 96 dias após a emergência para os espaçamentos de 80 e 90 cm, respectivamente, sendo que a queda de produtividade das parcelas mantidas no mato em relação às no limpo foi de mais de 80%, independentemente do espaçamento. / The research was conducted to evaluate the effect of reduced row spacing on the extension of interfeence periods on peanut yield (Arachis hypogaea cv. IAC Runner 886). The experiment was conducterd in Jaboticabal, SP, Brazil, on a Red- Latosol of medium texture. The treatments consisted of two row spacing (80 and 90 cm), divided in two groups. At the first one, the weeds were controlled since the emergence until 0 (kept with the weeds), 30, 45, 60, 82, 97 and 112 days after the peanut emergence. After those periods, the weed emerged and grew without control. At the second group, the weed grew since the peanut emergence during the same periods from the first group, however, at each period the weeds were removed from the plots. The experimental design was the split-plot scheme, being the main plots constituted by different row spacing and subplots constituted by periods of weedy or weed control, in a whole of 28 treatments with four replicates. The main weed at the area where: Digitaria sp., Xanthium strumarium, Acanthospermum hispidum and Cenchrus echinatus. To an acceptable yield loss of 5%, the critical period of interference control was from 27 to 76 and from 35 to 96 days after the emergence at the spacing rows of 80 and 90 cm, respectively, but the yield reduction at the non-controlled weed plots related to the plots free from interference was more than 90%, independent of the row spacing.

Submitted by Mario BC (mario@bc.ufrpe.br) on 2016-11-29T13:54:02Z No. of bitstreams: 1 Luciane de Nazare Almeida dos Reis.pdf: 1286764 bytes, checksum: e8ebbda7828d562331b43505ce6da593 (MD5) / Made available in DSpace on 2016-11-29T13:54:02Z (GMT). No. of bitstreams: 1 Luciane de Nazare Almeida dos Reis.pdf: 1286764 bytes, checksum: e8ebbda7828d562331b43505ce6da593 (MD5) Previous issue date: 2016-02-29 / The peanut (Arachis hypogaea L.) is an important source of protein and oil, and considered one of the most cultivated plants in Brazil and worldwide, that provide products consumed in different ways. It is the fourth world largest oleaginous in seed production, cultivated in over 30 countries, where China leads with 40.8%, followed by India with 14% and Nigeria with 7.5%. Brazil occupies the 17th position, concentrated in the Southeast and South regions, and the State of São Paulo accounts for 91.4% of national production. Among the factors that limit the development of this crop, there are several viruses, which cause reduction on productivity and the value of the product for marketing. Up to now, 31 viral species, classified in the following genera and respective families have been recorded naturally infecting peanut: Begomovirus (Geminiviridae), Bromovirus (Bromoviridae), Carlavirus (Betaflexiviridae), Cucumovirus (Bromoviridae), Irlavirus (Bromoviridae), Luteovirus (Luteoviridae), Pecluvirus (Virgaviridae), Potexvirus (Alphafleviridae), Potyvirus (Potyviridae), Rhabdovirus (Rhabdoviridae), Soymovirus (Caulimoviridae), Tospovirus (Bunyaviridae), Tymovirus (Tymoviridae) e Umbravirus (Tombusviridae). Of these genera, Potyvirus has more species. The advent of metagenomics, combined with high-performance technologies (Next Generation Sequencing - NGS) provides the exploration of micro-universe in various areas of science, including the Plant Virology. These techniques, together with bioinformatics, act as excellent tools for detection and characterization of novel viruses, as well as, all the viral genomes of different species or strains present in certain samples. The objective of this work was to study the virome of peanut plants exhibiting typical virus symptoms, obtained from producing areas of 10 counties of the State of São Paulo by NGS. The viral isolates were maintained by successive passages to peanut plants by grafting, under greenhouse conditions at the University of Brasilia (UnB). To perform the NGN, samples were semipurified aiming enrichment of virus particles, followed by total RNA extraction and sequencing carried out by Illumina platform. The metagenomic analysis permitted the detection the complete genome of Peanut mottle virus virus - PeMoV (Potyvirus) and Groundnut ringspot virus - GRSV (Tospovirus). Biological (indicator plants), serological (Dot-ELISA) and molecular (RT-PCR) tests were performed in order to characterize some GRSV isolates from peanut, which sequences were identified by metagenomic. Molecular analysis of the gene encoding the N protein, used for species taxonomy within Tospovirus genus, was also carried out with the selected isolates A1, N1 and O1. These three isolates induced symptomatic response in at least one of the indicator species (Datura stramonium L.) used in this work and widely cited in the literature. Positive results for these isolates were also confirmed by serology, indicating the presence of GRSV in peanut producing counties in the State of São Paulo. Phylogenetic analyzes of the segments L, M and S of tospoviruses revealed that the peanut GRSV isolates studied in this work grouped with sequences of GRSV isolate from watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) and a recombinant isolate of GRSV and Tomato chlorotic spot virus - TCSV obtained from tomato (Solanum lycopersicum L.). However, the phylogenetic analysis using the N protein sequence, demonstrated that the present peanut isolate grouped with sequences of GRSV isolates previously studied in Brazil. After phylogenetic analysis for proteins of all GRSV segments, it is believed that the species GRSV and TCSV share the same M segment. The comparison of PeMoV coat protein sequences revealed that the Brazilian peanut isolate reported in this work is more phylogenetically related with the isolate from South Korea, obtained from soybean (Glycine max L.) with 98% identity. This is the first report in Brazil and in the world of the complete genome sequences of PeMoV and GRSV in peanuts, by using NGS. / O amendoim (Arachis hypogaea L.) é uma importante fonte de proteína e óleo, sendo considerada uma das plantas mais cultivadas no Brasil e no mundo, com grande diversidade de formas de consumo de seus produtos. É a quarta oleaginosa com maior produção mundial, cultivada em mais de 30 países, em que a China lidera com 40,8%, seguida pela Índia com 14% e Nigéria com 7,5%. O Brasil ocupa a 17ª posição, concentrada nas regiões Sudeste e Sul, sendo o Estado de São Paulo responsável por 91,4% da produção nacional. Dentre os fatores que limitam o desenvolvimento dessa cultura, encontram-se diversos vírus, os quais ocasionam a redução da produtividade e valor do produto para comercialização. Até o momento, já foram registradas, infectando naturalmente o amendoim, 31 espécies virais, classificadas nos seguintes gêneros e respectivas famílias: Begomovirus (Geminiviridae), Bromovirus (Bromoviridae), Carlavirus (Betaflexiviridae), Cucumovirus (Bromoviridae), Irlavirus (Bromoviridae), Luteovirus (Luteoviridae), Pecluvirus (Virgaviridae), Potexvirus (Alphafleviridae), Potyvirus (Potyviridae), Rhabdovirus (Rhabdoviridae), Soymovirus (Caulimoviridae), Tospovirus (Bunyaviridae), Tymovirus (Tymoviridae) e Umbravirus (Tombusviridae). Destes, o gênero Potyvirus é o que agrega maior número de espécies. O advento da metagenômica, aliada às tecnologias de alto desempenho (Next Generation Sequencing - NGS) vêm propiciando a exploração do universo de microrganismos em várias áreas das ciências, inclusive na Virologia Vegetal. Estas técnicas, juntamente com a bioinformática, atuam como excelentes ferramentas para detecção e caracterização de novos vírus e de todos os genomas virais presentes em determinadas amostras, sejam de diferentes espécies ou estirpes. O objetivo desse trabalho foi realizar estudo de viroma de plantas de amendoim exibindo sintomas típicos de viroses, obtidas de áreas produtoras de 10 municípios do Estado de São Paulo por NGS. Os isolados virais foram mantidos por sucessivas passagens para plantas de amendoim por meio de enxertia, em casa de vegetação da Universidade de Brasília (UnB). Para a realização do NGS, as amostras foram semipurificadas objetivando um enriquecimento de partículas virais seguido de extração de RNA total e o sequenciamento, realizado pela Plataforma Illumina Miseq. A análise metagenômica permitiu a detecção do genoma completo do Peanut mottle virus - PeMoV (Potyvirus) e do Groundnut ringspot virus - GRSV (Tospovirus). Análises biológicas (plantas indicadoras), sorológicas (Dot-ELISA) e moleculares (RT-PCR) foram realizadas com intuito de caracterizar alguns isolados de GRSV de amendoim cuja sequência foi identificada por metagenômica. Análises moleculares do gene que codifica a proteína N, amplamente utilizado em trabalhos de taxonomia no gênero Tospovirus, foram também realizadas com os isolados selecionados A1, N1 e O1. Estes três isolados induziram reação sintomatológica em pelo menos uma das espécies indicadoras (Datura stramonium L.) utilizadas nesse trabalho e amplamente citada na literatura. Resultados positivos para estes isolados foram também confirmados por sorologia, evidenciando a presença de GRSV em municípios produtores de amendoim no Estado de São Paulo. Análises filogenéticas para os segmentos L, M e S de tospovírus revelaram que o isolado de GRSV de amendoim estudado neste trabalho agrupou-se com sequências de um isolado de GRSV de melancia (Citrullus lanatus (Thunb.) Matsum. & Nakai) e um isolado recombinante de GRSV e Tomato chlorotic spot virus - TCSV, obtido de tomate (Solanum lycopersicum L.). Entretanto, com a análise filogenética feita com a sequência da proteína N, ficou demonstrado que o presente isolado de amendoim se agrupou com sequências de isolados de GRSV previamente estudado no Brasil. Após análises filogenéticas para as proteínas de todos os segmentos de GRSV, acredita-se que as espécies de GRSV e TCSV compartilham o mesmo segmento M. Comparação das sequências da capa proteica de PeMoV, revelaram que o isolado brasileiro de amendoim relatado neste trabalho está mais relacionado filogeneticamente com um isolado obtido de soja (Glycine max L.), encontrado na Coréia do Sul, apresentando 98% de identidade. Este é o primeiro relato no Brasil e no Mundo das sequências do genoma completo de PeMoV e GRSV em amendoim, por meio do uso de NGS.