Wimax (Worldwide Interoperability for Microwave Access) is a standard of wireless metropolitan area networks (WMAN), designed by IEEE 802.16 standards group. The coverage of Wimax is wide, so it is suited for developing in large networks and provides more steady, high-speed, and secure data transmission for fixed and mobile subscribers. As the result, Wimax is designed for solving last mile problem because of the bandwidth is limit and the expensive building cost in the traditional networks. IEEE 802.16 (Wimax) defines two modes of data communication topology: PMP (Point-to-multipoint) mode and Mesh mode. In Mesh mode, the data can route through another subscriber station (SS), so it not only improves coverage range but also raises the throughput. Although IEEE 802.16 defined a secure sub layer in the MAC (Media Access Control) layer to provide privacy by encrypting connections between base station (BS) and subscriber station (SS), but it still face many security problems. In the mesh mode, it is not like PMP mode that the traffic is only between BS and SS, the traffic sometime will pass through another SS, for this reason, it is prone to bring many problems, such as shared secret key exposing, man-in-the-middle attack, eavesdropping threat¡Ketc. On the relay node (we termed sponsor node here), it also involved more unnecessary loads of encryption and decryption. In this thesis, we focus on the MAC layer , and we propose some schemes to modify the present of PKM and the traffic encryption key used for transmitting data, and we will show that these schemes can achieve better security than previous ones, also achieve efficient in data transmission.

Les travaux de recherche de ce manuscrit se concentrent sur l’analyse des fréquences de vibrations des robots. Nos applications concernent plus particulièrement les architectures à cinématique parallèle. Dans un premier temps nous avons considéré les robots parallèles redondants en actionnement pour lesquels nous envisageons d’augmenter la fréquence de leurs oscillations en utilisant les efforts internes intrinsèques à ce type de structure. L’objectif est d’utiliser leur actionnement pour mettre en tension leur structure, et par conséquent, par analogie avec une corde vibrante, augmenter la fréquence de leurs oscillations. Nous avons étudié plusieurs robots plans redondants et nous montrons que dans le cadre de robots typiquement conçus pour être rigides,l’influence des efforts internes rajoutés n’a que peu d’importance. La suite de nos travaux soutient la proposition suivante : "les trajectoires très dynamiques influencent les fréquences des oscillations de la plateforme mobile". En effet, les robots parallèles quand ils sont conçus pour être légers, peuvent atteindre de grandes accélérations. Nous avons choisi de nous intéresser à l’étude de l’impact que peut avoir les effets dynamiques sur la fréquence des oscillations de la plateforme mobile de ces robots. Les robots considérés pour nos développements sont des robots parallèles plans, redondants en actionnement ou non. Nous proposons d’étudier cette influence en nous basant sur un développement au premier ordre du modèle dynamique. Cette linéarisation du modèle dynamique se veut plus complète que celles proposées dans la littérature. Nous expliquons et vérifions la validité de notre approche par une étude sur le lien entre accélération et vitesse et la fréquence d’oscillation pour les robots série PR (pendule sur glissière verticale) et RR (double pendule en rotation horizontale). Ensuite, nous généralisons notre modélisation au premier ordre et l’appliquons aux quatre robots PRR-2 PRR-3, PRR-4 et Dual-V pour voir si nous sommes capable d’en dégager une tendance concernant l’évolution des fréquences d’oscillation. Nous constatons que, en fonction des trajectoires, la dynamique a une influence faible mais visible, souvent positive sur l’augmentation des fréquences d’oscillation de la plateforme mobile. Cependant, les trajectoires et les lois horaires étant imposées, nous ne pouvons que subir cette influence. / The research work of this thesis manuscript focus on the analysis of the frequency of robots’ vibrations. Our applications mainly revolve around architectures with parallel kinematics. First we examined parallel robots which are redundant in actuation and for which we are considering an increase of their oscillations’ frequency using the internal forces inherent to this type of structure. The aim is to use their actuation is the tensioning of their structure, and consequently, by analogy with a vibrating-wire, to enhance theiroscillation frequency. We have studied several redundancy planar robots and we demonstrate that in the case of robots which are typically designed to be stiff, the impact of added internal forces is of low relevance. The continuation of our research supports the following proposal: “High dynamics trajectories have an impact on the oscillation frequency of the mobile platform.” Indeed parallel robots, when designed to be light, can reach greater accelerations. We chose to concentrate on the study of the impact that dynamic effects canhave on the oscillation frequency of those robots’ mobile platform. The robots examined for our developments are planar parallel robots whether they have redundant actuation or not. We offer to study this impact based on a prime order development of the dynamic model. This linearisation of the dynamic model is intended to be more complete than those suggested by literature. We explain and verify the validity of our approach with a study on the link between speed and oscillation frequency on PR robots (pendulum on a vertical sliding guide) and RR robots ( double pendulum rotating horizontally). Then we will generalize our first order model and apply it to the four robots ( PRR-2 PRR-3, PRR-4, and Dual-V) to see if we are able to identify a pattern regarding the evolution ofoscillation frequencies. We observe that, depending on the trajectories, the dynamics have a low but noticeable, and often positive, impact on the increase of oscillation frequency of the mobile platform. However, since the trajectories and speed input laws are imposed, we have no choice but to be subjected to this impact.

As a major public health problem affecting more that 76.5 million Americans, chronic pain is one main reason why people seek medical attention. It is a pathological nervous system disorder that persists for months or years. Sensitization of nociceptive neurons in the dorsal horn of the spinal cord is crucial in the development of allodynia and hyperalgesia. The work presented in this thesis will focus on spinal protein kinase M zeta (PKMζ)-mediated plasticity and GABAergic disinhibition as spinal amplification mechanisms that orchestrate persistent changes in the dorsal horn of the spinal cord. As a result of central sensitization following peripheral nerve injruy, GABAergic disinhibition occurs due to an alteration in Cl- homeostasis via reduced KCC2 expression and function. Intrathecal administration of acetazolamide (ACT), a carbonic anhydrase inhibitor, attenuated neuropathic allodynia and spinal co-adminitation of ACT and midazolam (MZL), an allosteric modulator of the benzodiazepine class of GABAA receptors, synergistically inhibited neuropathic allodynia. Further studies concerning the impact of altered Cl-homeostasis via reduced KCC2-mediated Cl-extrusion capacity on the analgesic efficacy and potency of GABAA receptor agonist and allosteric modulators revealed that there is a differential regulation of the agonists and allosteric modulators at the GABAA receptor complex when Cl-homeostasis is altered. Another spinal amplification mechanism leading to central sensitization is PKMζ-mediated spinal LTP. In model of persistent nociceptive sensitization, allodynia induced by IL-6 injection or plantar incision was abolished by both the inhibition of protein translation machinery and PKMζ inhibitor, ZIP. However, only PKMζ inhibition prevented the enhanced pain hypersensitivity precipitated by a subsequent stimulus after the initial hypersensitivity had resolved, asserting that spinal PKMζ underlies the maintenance mechanisms of persistent nociceptive sensitization. Also, these results confirmed that the initiation mechanisms of persistent sensitization parallel LTP initiation mechanisms and the maintenance mechanisms of persistent sensitization parallel LTP maintenance mechanisms. Taken together, these results indicate that these amplification mechanisms drive a chronic persistent state in these models such that inhibition of these spinal amplication mechanisms will serve as an effective approach in the quenching chronic pain hypersensitivity in chronic pain models.

Learning and memory has been a topic that has captured the attention of the scientific and public communities since the dawn of scientific discovery. Without the faculty of memory, mammals cannot experience nor function in the world; among homosapiens specifically, language, relationships, and personal identity cannot be developed (Eysenck, 2012). After all, some philosophers such as John Locke argued we are nothing but a collection of past memories in which we have developed and improved upon (Nimbalkar, 2011). Understanding the cellular mechanisms behind learning, and the subsequent formation of memory, has been a topic that has garnered scientific interest for many decades. One particular kinase that has been at the center of attention in the last decade is the serine/threonine kinase PKM-ζ, an N-terminal truncated form of PKC-ζ that renders it constitutively active (Hernandez et al., 2003). PKM-ζ has long been implicated in a cellular correlate of learning, long-term potentiation (LTP). Inhibition of PKM-ζ with Zeta-inhibitory peptide (ZIP) has been shown in many brain structures to disrupt maintenance of AMPA receptors, irreversibly disrupting numerous forms of learning and memory that have been maintained for weeks. The voltage-gated potassium channel Kv1.2 is a critical modulator of neuronal physiology, including dendritic excitability, action potential propagation, and neurotransmitter release. While expressed in various mammalian tissues, Kv1.2 is most prevalent in the cerebellum where it modulates both dendritic excitability of Purkinje cells (PCs) and basket cell (BC) inhibitory input to PCs. Because PCs are the main computational unit of the cerebellar cortex and provide its sole output (Napper et al., 1988; Harvey et al., 1991), regulation of synaptic Kv1.2 is predicted to have a major role in cerebellar function. Pharmacological inhibition of Kv1.2 in cerebellar PC dendrites increases excitability (Khavandgar et al., 2005), while its inhibition in BC axon terminals increases inhibition to PCs (Southan & Robertson, 1998). Interestingly, two prior studies have demonstrated that PKC-ζ, an atypical Protein Kinase C, is able to phosphorylate and bind cerebellar Kvβ2, a Kv1.2 auxiliary subunit. (Gong et al., 1999; Croci et al., 2003). Delay eyeblink conditioning (EBC) is an established model for the assessment of cerebellar learning. Despite being highly expressed in the cerebellum, no studies have examined how regulation of cerebellar PKM-ζ may affect cerebellar-dependent learning and memory nor have they examined the possible effect PKM-ζ may have on Kv1.2. The goal of this dissertation was to determine whether PKM-ζ could modulate EBC in a Kv1.2 dependent manner. Through the use of microscopy techniques we have shown that PKM-ζ is highly expressed in the cerebellar cortex, primarily in the PC, and by the use of pharmacological manipulations, it was found that PKM-ζ has an important role in regulating the acquisition of EBC. Through the use of biotinylation, flow cytometry, and behavioral manipulations, it was determined that PKM-ζ regulates Kv1.2 during eyeblink conditioning. These studies provided the first evidence that PKM-ζ has a role for learning and memory in the cerebellum, and the first evidence of PKM-ζ regulating a voltage-gated ion channel rather than a ligand-gated ion channel such as AMPA receptors.

The aim of using parallel kinematic motion systems as an alternative of conventional machine tools for precision machining has raised the demands made on the accuracy of identification of the geometric parameters that are necessary for the kinematic transformation of the motion variables. The accuracy of a parallel manipulator is not only dependent upon an accurate control of its actuators but also upon a good knowledge of its geometrical characteristics. As the platform's controller determines the length of the actuators according to the nominal model, the resulted pose of the platform is inaccurate. One way to enhance platform accuracy is by kinematic calibration, a process by which the actual kinematic parameters are identified and then implemented to modify the kinematic model used by the controller. The first and most general valuation criterion for the actual calibration approaches is the relative improvement of the motion accuracy, eclipsing the other aspects to pay for it. The calibration outlay has been underestimated or even neglected for a long time. The scientific value of the calibration procedure is not only in direct proportion to the achieved accuracy, but also to the calibration effort. These demands become particularly stringent in case of the calibration of hexapods of the so-called simple design. The objectives of the here proposed new calibration procedure are based on the deficits mentioned above under the special requirements due to the circumstances of the simple design-concept. The main goals of the procedure can be summarized in obtaining the basics for an automated kinematic calibration procedure which works efficiently, quickly, effectively and possibly low-cost, all-in-one economically applied to the parallel kinematic machines. The problem will be approached systematically and taking step by step the necessary conclu-sions and measurements through: Systematical analysis of the workspace to determine the optimal measuring procedure, measurements with automated data acquisition and evaluation, simulated measurements based on the kinematic model of the structure and identifying the kinematic parameters using efficient optimization algorithms. The presented calibration has been successfully implemented and tested on the hexapod of simple design `Felix' available at the IWM, TU Dresden. The obtained results encourage the application of the procedure to other hexapod structures.

No description available.

The generation of macrophages requires the coordinated responses to stimulatory and inhibitory signals that cell receive from their environment. While the up-regulation of macrophage production and survival is essential to fortify the immune system, their down-regulation is also vital to prevent macrophage related diseases and malignancy. Previous studies in goldfish showed that primary kidney macrophages release endogenous growth inducing factors into proliferative phase supernatant, which up-regulate their proliferation and survival. In this thesis, the effect of senescence phase supernatant (collected from goldfish primary kidney macrophage cultures) on goldfish primary kidney macrophages proliferation, survival and its impact on the ability of macrophages to tolerate H2O2 was analyzed. The results showed that the senescence phase supernatant down-regulated the proliferation and survival, and decreased the chemical tolerance of the cells. This indicated that the goldfish primary kidney macrophages promote targeted control of their proliferation and survival by secreting endogenous growth inhibitory factors in the senescence phase supernatant. / Physiology, Cell and Development Biology

No description available.

Internet-based and mobile technologies enable new ways of learning. They offer us new possibilities to access an enormous amount of knowledge at any time and everywhere. Among many advantages, the adaptations require a rethinking of our previous learning behaviour patterns and processes. The challenge for students is no longer to get access to information and knowledge, but to select the right one and to deal with the information and knowledge overflow. The aim of this research is to define, design and validate an advanced concept to support the contemporary learning processes. Therefore, the requirements for a new approach have been assessed, the available solutions from the related area of (personal) Knowledge Management have been investigated, and the weaknesses in the context of learning identified. The identified issues have been substantiated by university students via a quantitative survey. Besides several smaller aspects, knowledge fragmentation and the nescience of the knowledge formation process have been classified as the most critical ones. To overcome these problems, a methodological concept has been developed, and a corresponding technological design created. The chosen approach is an intelligent, independent intermediate layer, which traces the different steps our knowledge entities are going through. Based on personal and individual configurations, the system provides a comprehensive and overall observation of nearly all our knowledge work activities. It supports the building and accessing of the knowledge formation paths for every important knowledge unit, later path combination and the access to automatically generated versions of our work. Moreover, it helps the users not only to remember what they did, but also gives them some strong indications why they did it. This is achieved by combining different knowledge actions and looking at the influences they have on each other. The suggested concept has been critically proved and confirmed via a qualitative expert analysis and backed up by a quantitative survey among university students.

Este trabalho trata da avaliação do desempenho de máquinas de usinagem cujas arquiteturas se baseiem em estruturas cinemáticas paralelas planas. Inicialmente analisa dois tipos distintos de arquiteturas paralelas, avaliando as suas mobilidades, possíveis formas de atuação, além de apresentar seus modelos teóricos cinemáticos e dinâmicos correspondentes. A seguir, descrevem-se no texto algumas simulações realizadas em oito modelos propostos, de modo a se observar os comportamentos das arquiteturas consideradas, quando da execução de possíveis trajetórias da ferramenta em movimento plano (2D). A seleção dos modelos e a discussão dos resultados são guiadas por índices de desempenho presentes na literatura e outros propostos ou adaptados. O trabalho discute ainda as melhores soluções, com foco nas trajetórias prédefinidas e em critérios importantes para os mecanismos de arquitetura paralela. / This dissertation concerns the performance evaluation of machine-tool with architectures based on planar parallel kinematic structures. The text starts analyzing two distinct types of parallel architectures, their mobility, feasible schemes of actuation and presents their kinematic and dynamic models. From this point, simulations are described to figure out some characteristics of chosen architectures, in eight different models, focused on execution of tool trajectories in plane motion (2D). The model selection and the discussion about obtained results are guided by performance indexes from the literature and others either proposed or adapted. The work also discusses the best solutions, for pre-defined tool trajectories and important points for mechanisms with parallel structures.