Previous studies on microtaphonomy have identified multiple different organic microstructures in fossil vertebrates from a variety of time periods and environmental settings. This study seeks to investigate the potential taphonomic, paleoclimatic, and geochemical controls on soft tissue and cellular microstructure preservation. To this end, fifteen vertebrate fossils were studied: eight fossils collected from the Oligocene Sharps Formation of the Arikaree Group in Badlands National Park, South Dakota, and seven fossils from formations in the underlying White River Group, including the the (Oligocene) Brule Formation of Badlands National Park, and the (Eocene) Chadron Formation of Flagstaff Rim, Wyoming; Toadstool Geologic Park, Nebraska; and Badlands National Park, South Dakota. A portion of each fossil was demineralized to identify any organic microstructures preserved within the fossils. I investigated several potential soft tissue preservation factors, including taxonomy, paleoclimate, depositional environment, and diagenetic history as shown through bone apatite crystallinity and trace element alteration. Soft tissue microstructures were preserved in all fossil samples, and cellular material was recovered from most fossil specimens. Soft tissue and cellular preservation was found to occur independent of taxonomy, paleoclimate regime, depositional environment, and apatite crystallinity. The period of fossilization and diagenetic trace element addition, as shown through rare earth element (Lanthanum) diffusion profiles, may be connected to organic microstructure preservation, as longer estimated diffusion periods were correlated with poorer preservation of bone histology and greater cellular degradation in some of the fossil samples. / Geology / Accompanied by one Excel file: Thesis XRD Driffractograms Full.

The requirement for identical inverted terminal repeats (ITRs) for viral viability and the role of internal viral sequences in the specification of the sequences of the termini were investigated. The viral strains used in this study were a variant Ad2 strain Ad2 (mac) and the wild type Ad5 strain which was very similar to the former one in sequence except at the extreme end of the terminal repeat. A hybrid virus (sub54), obtained by recombination between Ad2 (mac) and Ad5, derived the left 41-51% of its genome from Ad2 (mac) and the right 59-49% from Ad5. The identity of the termini was determined by Southern blotting analysis using 32p end labeled oligocleoxynucleotides. Analysis of the sub54 isolate indicated that both Ad2 (mac) and Ad5 ITRs were present. Plaque purification of sub54 demonstrated that viruses with non identical terminal sequences were viable and allowed their characterization. This analysis also indicated that Ad5 ITRs are converted to Ad2 (mac) ITRs possibly as a result of repair of the ends to yield viruses with identical termini. A model involving replication and emphasizing the importance of panhandle formation as a replicative intermediate is proposed. These results also indicated a possible role of the internal sequences of adenovirus in the selection and maintenance of serotype specific ITRs. The preference for Ad2 (mac) termini observed during repair of the ends of sub54 may be related to the origin of the genes coding for the adenoviral polymerase and/or the terminal protein both of which were derived from Ad2 (mac). Further investigation would be required to determine whether these replicative proteins are actually involved in ITR conversion. Transformation of Escherichia coli with a DNA preparation from sub54 infected rat embryo cells resulted in the isolation of the plasmid pFG154. This plasmid contained the entire adenovirus genome with an Ad2 (mac) ITR at the "left" terminus covalently linked to an Ad5 ITR at the "right". Analysis of the viral progeny generated upon transfection of mammalian cells with pFG154 indicated that the Ad2 (mac) ITRs were very efficiently converted to Ad5 termini. These results, although apparently contradictory to those initially obtained from the plaque purification of sub54, may be explained by an ITR repair model which is specific for infectious circles. / Thesis / Master of Science (MS)

Recognition for historic preservation work in the United States has been reserved almost exclusively for the white elites who have dominated the preservation movement since the mid-nineteenth century. In contrast, this interdisciplinary dissertation presents Black men and women operating outside of the white preservation mainstream and recounts narratives of Black Bostonians stewarding buildings, landscapes, and neighborhoods. Several arguments emerge from the four case studies. First, Black Bostonians engaged in preservation work. Second, Black preservation efforts were useful as ways to channel place-based history into reform efforts predicated on the need for change, running counter to white elites' bolstering of continuity and celebratory histories. Third, Black preservation strategies, like their interpretations of the sites, incorporated and embraced change that was expressed in variations of adaptive reuse. Fourth, different factions within the city’s Black population had different preservation interests and visions of change, and whites and Blacks often took interest in the same sites for divergent reasons. And, finally, this dissertation shows that the white mainstream has repeatedly slighted Black preservation strategies and even gone so far as to cast them as blighting actions. Chapter One centers on the group of elite Black Bostonians who purchased the Roxbury home of William Lloyd Garrison, known as “Rockledge,” with the intent to preserve it as an antislavery memorial at the turn of the twentieth century. As St. Monica's Home for Colored Women and Children, the house became a site of contestation between the followers of William Monroe Trotter and Booker T. Washington. Chapter Two examines the dispute over the future of the Charles Street Meeting House, then the Charles Street A.M.E. Church, when a proposed street widening threatened the building in 1920. Chapter Three makes a case for studying actions that result in preservation and not just people who identify as preservationists by examining the conversions of single-family houses to multi-family homes by Black middle-class homeowners from the 1930s through the early 1960s. Lastly, Chapter Four features Elma Lewis, a Black arts educator and cultural activist who in 1966 adapted the Overlook Shelter ruins in Frederick Law Olmsted’s Franklin Park into an outdoor theater to support her community.

This study examines policy and procedure documents related to Disability at 3 U.S. institutions of higher education over a 25-year time frame. Policy and procedure documents are the foundation that govern how institutions "handle" Disability, outlining expectations and guidelines for providing services and establishing bureaucratic channels used to determine who has access to those services. This research employs a comparative case study mixed methods approach. The found documents and their online contexts are analyzed according to four qualities: findability, cohesion, consistency, and transparency. A document's findability refers to the ability of a user to locate the original document, and a document's cohesion, consistency, and transparency, refer to respectively where, what, and how these documents persist from their original creation date. As I collected these documents, I constructed comparative matrices to track these qualities within and across three different universities. The initial findability of documents demonstrates two key results: 1) during the overall 1990– 2015 time frame, there was a marked change in the availability of materials in a digital format, and 2) the emergence of a way to describe documents via the phrase "Does Not Exist." These materials definitively did not exist prior to a given time frame, but later versions of such documents included an earlier start date. Cohesion results indicate that the documents most likely to be presented in a single source were broadly usable to a large portion of the university population: the general student body. Consistency results address a major issue with the document search: while these materials were likely to exist, at each of these institutions and time frames (barring the DNE documents), they are very difficult to track down. Transparency across found, single-source documents was ubiquitous; if it could be found, it had searchable text. Beyond the findings of my document collection, I created two major products as a result of this dissertation work: key recommendations for different stakeholder groups and a curated exhibit of VT-specific materials collected for this study. / Doctor of Philosophy / This study examines policy and procedure documents related to Disability at 3 U.S. institutions of higher education over a 25-year time frame. Policy and procedure documents are the foundation that govern how institutions "handle" Disability, outlining expectations and guidelines for providing services and establishing bureaucratic channels used to determine who has access to those services. This research employs a comparative case study mixed methods approach. The found documents and their online contexts are analyzed according to four qualities: findability, cohesion, consistency, and transparency. A document's findability refers to the ability of a user to locate the original document, and a document's cohesion, consistency, and transparency, refer to respectively where, what, and how these documents persist from their original creation date. As I collected these documents, I constructed comparative matrices to track these qualities within and across three different universities. The initial findability of documents demonstrates two key results: 1) during the overall 1990– 2015 time frame, there was a marked change in the availability of materials in a digital format, and 2) the emergence of a way to describe documents via the phrase "Does Not Exist." These materials definitively did not exist prior to a given time frame, but later versions of such documents included an earlier start date. Cohesion results indicate that the documents most likely to be presented in a single source were broadly usable to a large portion of the university population: the general student body. Consistency results address a major issue with the document search: while these materials were likely to exist, at each of these institutions and time frames (barring the DNE documents), they are very difficult to track down. Transparency across found, single-source documents was ubiquitous; if it could be found, it had searchable text. Beyond the findings of the document collection, there are two major products as a result of this dissertation work. First, key recommendations for different stakeholder groups (SEEKERS, WRITERS, and KEEPERS) are outlined; these recommendations are intended for the entire audience as practices that they can incorporate within their own documents. Second, the work undertaken to create a repository using materials from my document collection, utilizing the Qualitative Data Repository (based in Syracuse University) as the host for a curated exhibit of VT-specific materials, is described.

Web archiving is necessary to retain the history of the World Wide Web and to study its evolution. It is important for the cultural heritage community. Some organizations are legally obligated to capture and archive Web content. The advent of transactional Web archiving makes the archiving process more efficient, thereby aiding organizations to archive their Web content. This study measures and analyzes the performance of transactional Web archiving systems. To conduct a detailed analysis, we construct a meaningful design space defined by the system specifications that determine the performance of these systems. SiteStory, a state-of-the-art transactional Web archiving system, and local archiving, an alternative archiving technique, are used in this research. We experimentally evaluate the performance of these systems using the Greek version of Wikipedia deployed on dedicated hardware on a private network. Our benchmarking results show that the local archiving technique uses a Web server’s resources more efficiently than SiteStory for one data point in our design space. Better performance than SiteStory in such scenarios makes our archiving solution favorable to use for transactional archiving. We also show that SiteStory does not impose any significant performance overhead on the Web server for the rest of the data points in our design space. / Master of Science

This thesis establishes a guideline for the future preservation, management and interpretation of Brownsville Plantation. Brownsville Plantation is located in Northampton County on Virginia's lower Eastern Shore. Brownsville's original 1262 acre parcel of land was first patented in 1652 by English Quakers, John and Ursula Browne. At the time of this research (July, 1995 to March, 1996) the property was held in ownership by The Nature Conservancy's Virginia Coast Reserve. The passing of significant evidence of our history to future generations, provides us with the unique opportunity to both examine and interpret our shared cultural resources. Brownsville's significance lies in the intricate weaving of the natural fabric of the site with the human cultural activities which have historically been exhibited there. The property's significance cannot be quantified as only land or only building(s), instead it is the epic of a place in which each component is inseparable from the entirety of its history. The methodology implemented in developing this thesis includes four separate but interconnected phases of work. Each phase is founded in linking traces of human activity, as physical representations of the property's cultural heritage, and endeavors to recognize the present and future of Brownsville by acknowledging the past. The phases are: Historical Research; Inventory & Documentation; Site Analysis & Evaluation; and Treatment Selection & Recommendations. Each places the site within an historic and current context while determining stewardship strategies and priorities based on the property's retained overall integrity. As a comprehensive plan of action, this work is envisioned as an interim step in the continuum which forms the property's history. It is intended as a tool which assists in the management of change, while providing clear attitudes for future exploration and ongoing research at Brownsville Plantation. / Master of Science

Exploration of the architectural re-purposing of a historic building ruin with the goal of maintaining and emphasizing its current character and state of decay. Simultaneously examining how new interventions can clearly achieve modern expectations of function and enclosure, while allowing the character of the existing structure to be minimally impacted. The intervention in the historic building fabric demonstrates how new materials and construction techniques allow for a distinct contrast between old and new, and how the two can work harmoniously together. Specifically integrates new glued-laminated timber frame structure in contrast to existing concrete walls, testing a general case for the potential power of interventions and adaptive reuse to be a means to a rebirth and retelling of the stories of existing buildings. / Master of Architecture

The long-term preservation of digital objects has become increasingly relevant. Libraries, public institutions and museums, but also companies are requesting solutions to store their digital files with all relevant contents and attributes for the future. This master thesis makes two contributions to the research in digital preservation.The first attempt is the creation of a testbed which stores many files in different file formats. These files can be used to evaluate the impact of preservation solutions. In this paper an environment for storing and describing files is being suggested and implemented.The second contribution is made by presenting a framework which is based on Utility Analysis for evaluating different preservation solutions. The application of a detailed hierarchy of objectives, considering the individual requirements of the user, will allow a reasonable and clear decision for a specific preservation solution, which can be supported with arguments. The theoretic framework is evaluated in two casestudies. For the first one the whole process is being realized, for the second example only the major part of the analysis, the objective tree, is treated in detail.

The Conservation easement has become a popular tool for land protection in the past few decades. Whether this development restriction will necessarily decrease the land value is an empirical question. This study employs a hedonic pricing approach to test empirically the effects of conservation easements on land values. The econometric results indicate that conservation easements can slightly increase the land values, but the effect is statistically insignificance. Considering the limited dataset, the interpretation of the results warrant some caution. / Master of Science

This thesis proposes guidelines for reestablishing the historic canal system on Rattanakosin Island, Bangkok as a public space system and a connector of key public spaces. The study examines the historic value and cultural symbolism of the canals through evolutionary morphological analysis, establishing that the canals are primary structural elements in the city, since they have retained the integrity of their physical form over time, and have come to hold cultural meaning for the Thai people. The canals have also accommodated different functions over time, in response to a changing urban context. There is potential for them to accept new functions as recreational spaces, connectors of key public spaces, and as tourist destinations. Typological analysis of structural characteristics of the canals yields seven canal types that have potential to accommodate public space functions. The study proposes guidelines for the seven canal types that will enhance these potentials and ensure the preservation of the canals' physical form. / Master of Landscape Architecture