This dissertation examined recently issued regulatory standards using cheese and produce as model systems. FDA's 2015 Domestic and Imported Cheese and Cheese Products Compliance Program Guidelines (CPG) E. coli standards on cheese safety, and the extent to which these standards affect domestic and imported cheese commerce, was assessed. Results from FDA's Domestic and Imported Cheese Compliance Program for samples collected between January 1, 2004 and December 31, 2006 were analyzed. Of 3,007 cheese samples tested for non-toxigenic E. coli, 76% (2,300) of samples exceeded 10/g, FDA's target for regulatory activity. In cheese samples containing E. coli levels of 10/g and 100/g, there was no statistically significant association with presence of Listeria monocytogenes. However, associations between Staphylococcus aureus levels of 10,000 CFU/g and presence of Salmonella and L. monocytogenes were statistically significant, indicating that EU regulations targeting S. aureus as the pathogen of concern may be more appropriate than E. coli for cheese safety assessment. Compost amended soils in the Northeastern U.S. were assessed for the presence and survival of E. coli and Listeria spp. against FDA Food Safety Modernization Act (FSMA) requirements. Manure and poultry litter-based biological soil amendments of animal origin (BSAAO) must achieve pathogen reduction to reduce risk of pathogen contamination on the harvested produce. Two trials of replicated field plots of loamy (L) or sandy (S) soils were tilled and un-amended (NC) or amended with dairy compost (DC), poultry litter compost (PL), or poultry pellets (PP). Colony count and most probable number (MPN) methods were used to determine persistence of E. coli in these plots over 104 days post-inoculation. Detection of indigenous Listeria spp. were also examined in all plots. Higher E. coli populations were observed in PL and PP amended soils when compared to DC and NC plots. E. coli was detected at low levels on radish crops, where PL treatments encouraged greater levels of survival and growth than DC or NC. Study results verify that a 120 day interval following BSAAO application should be sufficient to ensure food safety of edible crops subsequently planted on these soils. The sensitivity of environmental monitoring methods and collection formats were evaluated to identify optimal procedures for detection of Listeria spp. on product contact surfaces within artisan cheese production environments. Four environmental surfaces (dairy brick, stainless steel, plastic, and wood; n=405/surface type) were inoculated with L. innocua, L.m. ATTC® 19115 and L.m. 1042, at high (106-107/cm2) and low (0.1-1/cm2) target concentrations. Inoculated surfaces were swabbed with World Bioproducts© EZ ReachTM and 3MTM environmental swabs (3MTM). Five enrichment and enumeration methods were used to compare sensitivity of recovery between environmental swabs. All swab formats performed equally on all environmental surfaces at high target concentrations. At low concentrations, PetrifilmTM and WBEZ swabs recovered Listeria spp. from 87.5% of plastic, stainless steel, and dairy brick surfaces, but only 62.7% of wooden surfaces; recovering 14.8%, 77%, and 96.3% of cells from initial inoculations of 0.01, 0.1, and 1/cm2, respectively . Our data demonstrate that results may be discrepant due to variation in the porosity of environmental surfaces and should be taken into consideration when implementing environmental sampling plans. Results from this thesis can be used to inform regulatory policy and help to achieve improved food safety.

Thesis (MSc. (Agriculture)) --University of Limpopo, 2011 / Maize is the most important cereal crop grown in South Africa. This crop is produced throughout the country under diverse environments. The study only focuses on the technical efficiency because it is an important subject in developing agriculture where resources are limited, but high population growth is very common. Technical efficiency is the ability of a farmer to obtain output from a given set of physical inputs. Farmers have a tendency of under and/or overutilising the factors of production. The main aim of this study was to analyse the technical efficiency of small-scale maize producers in Ga-Mothiba rural community of Limpopo Province. The objective of the study was to determine the level of technical efficiency of smallscale maize producers and to identify the socio-economic characteristics that influence technical efficiency of small-scale maize producers in Ga-Mothiba. Purposive and Snowball sampling techniques were used to collect primary data from 120 small-scale farmers. Cobb-Douglas production function was used to determine the level of technical efficiency and Logistic regression model was used to analyse the variables that have influence the technical efficiency of maize production. Cobb-Douglas results reveal that small-scale farmers in Ga-Mothiba are experiencing technical inefficiency in maize production due to the decreasing return to scale, which means they are over-utilising factors of production. Logistic regression results indicate that out of 13 variables included in the analysis as socio-economic factors, 10 of them (level of education, income of the household on monthly basis, farmer`s farming experience, farm size, cost of tractor hours, fertiliser application, purchased hybrid maize seeds, membership to farmers` organisation, is maize profitable) were found to be significant and 3 (gender, age and hired labour) are non-significant. However, farm size was found to be the vi most significant variable at 99% level, showing a positive relationship to smallscale maize producer`s technical efficiency. Therefore, it is recommended that government should do the on-farm training since farmers mainly depend on trial and error and farmers` should have access to enough arable land and tractor services. However, farmers need to be trained on matters relating to fertiliser application, on the amount of seeds a farmer should apply per ha, and the importance of using hybrid seed.

Thesis (M.Dev.) --University of Limpopo, 2007

Robotics has been considered as a viable automation solution for the aerospace industry to address manufacturing cost. Many of the existing robot systems augmented with guidance from a large volume metrology system have proved to meet the high dimensional accuracy requirements in aero-structure assembly. However, they have been mainly deployed as costly and dedicated systems, which might not be ideal for aerospace manufacturing having low production rate and long cycle time. The work described in this thesis is to provide technical solutions to improve the flexibility and cost-efficiency of such metrology-integrated robot systems. To address the flexibility, a software framework that supports reconfigurable system integration is developed. The framework provides a design methodology to compose distributed software components which can be integrated dynamically at runtime. This provides the potential for the automation devices (robots, metrology, actuators etc.) controlled by these software components to be assembled on demand for various assembly applications. To reduce the cost of deployment, this thesis proposes a two-stage error compensation scheme for industrial robots that requires only intermittent metrology input, thus allowing for one expensive metrology system to be used by a number of robots. Robot calibration is employed in the first stage to reduce the majority of robot inaccuracy then the metrology will correct the residual errors. In this work, a new calibration model for serial robots having a parallelogram linkage is developed that takes into account both geometric errors and joint deflections induced by link masses and weight of the end-effectors. Experiments are conducted to evaluate the two pieces of work presented above. The proposed framework is adopted to create a distributed control system that implements calibration and error compensation for a large industrial robot having a parallelogram linkage. The control system is formed by hot-plugging the control applications of the robot and metrology used together. Experimental results show that the developed error model was able to improve the 3 positional accuracy of the loaded robot from several millimetres to less than one millimetre and reduce half of the time previously required to correct the errors by using only the metrology. The experiments also demonstrate the capability of sharing one metrology system to more than one robot.

Fresh produce is increasingly implicated in outbreaks of foodborne illness. Internalization of bacterial pathogens into produce is of particular concern as internalized pathogens are unlikely to be removed by surface sanitizers. It is therefore necessary to develop treatments that will reduce their prevalence and numbers on fresh produce. Irradiation is a penetrating nonthermal treatment that effectively eliminates bacteria. Irradiated baby spinach leaves up to 1.0 kGy showed negligible (P>0.05) changes in color, texture, vitamin C, total carotenoids, and chlorophyll content compared to non-irradiated controls throughout storage (15 days at 4oC). This research also shows that irradiation effectively reduces viable Escherichia coli cells internalized in lettuce, and that decontamination is not influenced (P>0.05) by lettuce variety. Irradiation effectively reduced the population of internalized pathogens in a dose-dependent manner (3-4 log reduction at 1.0 kGy). Microscopy images suggest that the contamination sites of pathogens in leafy vegetables are mainly localized on crevices and in the stomata. A careful design of the treatment (understanding dose distribution) will effectively eliminate pathogens while maintaining produce quality. The use of modified atmosphere packaging increased (P<0.05) the sensitivity of pathogens (Salmonella spp. and Listeria spp.) to irradiation in baby spinach leaves (up to 25%). Increasing concentration of oxygen increased (P<0.05) sensitivity of both microorganisms. Radiosensitization could be affected (P<0.05) by production of ozone, which increases with increasing dose-rate and oxygen concentration, and reducing temperatures. Antimicrobial effectiveness of various active compounds was determined against Salmonella spp. and Listeria spp. Inclusion complexes were prepared with antimicrobial compounds and -cyclodextrin. The effectiveness of the microencapsulated compounds was tested by spraying them on the surface of baby spinach leaves inoculated with Salmonella spp. The increase in radiosensitivity (up to 40%) varied with the antimicrobial compound. Spherical poly (DL-lactide-co-glycolide) (PLGA) nanoparticles with entrapped eugenol and trans-cinnamaldehyde were synthesized for future antimicrobial delivery applications. All loaded nanoparticles proved to be efficient in inhibiting growth of Salmonella spp. and Listeria spp. The entrapment efficiency for eugenol and trans-cinnamaldehyde was 98% and 92%, respectively. Controlled release experiments (in vitro at 37oC for 72 hrs) showed an initial burst followed by a slower release rate of the antimicrobial entrapped inside the PLGA matrix.

No description available.

In recent years the three Prairie Pools have actively expanded their primary operations to include a number of investments both within and outside of the agricultural sector. The Pools' investment strategies are economically interesting because they are being pursued within the context of a co-operative organizational structure which requires that the users of the cooperative business also own, control, and benefit from its operations. This thesis examines the possible economic incentives agricultural co-operatives may have to invest in vertically integrated and diversified activities using the case of the Western Canadian co-operative elevator companies as an example. The analysis undertaken in this thesis is structured in two ways. First, the economic literature regarding co-operative formation and conventional firm expansion is surveyed. This analysis suggests that an important difference between vertically integrated investments and diversified investments is that they are motivated by the realization of distincly different sets of economic benefits for the co-operative firm and its members. It is argued that co-operative vertical integration can convey benefits to members indirectly through the market, in the form of increased producer margins and improved market access. However, these benefits may not impact the "bottom line" of the co-operative firm. Diversification can, on the other hand, provide a co-operative with direct monetary benefits in the form of improved financial performance and increased profits, which can translate into increased patronage refunds available to members. The second component of this analysis involves the development of a simulation model to examine the implications of an additional hypothesis proposed to explain co-operative expansion. The proposed hypothesis is based on the notion that perhaps the indirect market benefits from cooperation and co-operative expansion are being undervalued. This undervaluation can result in a preoccupation with the monetary benefits from co-operative business, and may therefore cause a bias towards diversified investments. The model developed in this thesis illustrates that, although such a bias may improve a co-operative's rate of return, it may also result in significant opportunity costs for agricultural producers due to a decrease in a co-operative's pro-competitive effect on primary markets.

Darbo objektas – pušų, eglių, beržų, alksnių medžių stiebai ir jų iš pagaminta apvaliosios medienos produkcija. Darbo tikslas – Išanalizuoti medienos tūrio skirtumus, kurie susidaro medienos apskaitai naudojant medžių stiebų su žieve tūrio, medžių tūrio struktūros ir rąstų tūrio lenteles. Darbo metodai – statistiniai, empiriniai. Darbo rezultatai. Atlikus tyrimus buvo gauna, kad stiebų tūrio lentelės vidutiniškai 2,2% didina visų medžių rūšių kartu paėmus stiebų su žieve tūrius. Stiebų tūrio lentelės vidutiniškai 4,4% didina pušų stiebų su žieve tūrį, ir 2,5% mažina juodalksnių stiebų su žieve tūrį. Tikrintų biržių imčių duomenys rodo, kad visų medžių rūšių kartu paėmus likvidikės medienos tūris, nustatytas pagal medžių tūrio struktūros lenteles, yra 3.5% padidintas. Vidutinis nelikvidinės medienos tūris bareliuose sudaro 15%. Rąstų tūrio lentelių tikslumas yra pakankamas apvalios medienos tūriui įvertinti. Tūrio skirtumo paklaida yra neesminė. Raktažodžiai: Stiebai, mediena, produkcija, tūrio skirtumai, žievė. / Work object - stems of pinus, picea, alnus and betula and from these produced round wood production. Work goal - to compare stems capacity of the same trees, which is evaluate by trees stems with bark tables with capacity, which is evaluated by compound Huber formula, by structure of trees capacity tables and by capacity of logs tables. Work methods – statisticals, empiricals. Work results – after research was noticed that tables of stems capacity increases all kinds of trees capacity including stems with bark about 2,2%. Tables of stems capacity about 4,4% increase volume of pinus stems with bark and about 2,5% reduce volume of alnus stems with bark. The material of checked plots shows that all kinds of trees liquidated wood capacity, which was evaluated by tables of trees capacity structure is increased 3.5%. Medium not commercial wood capacity in areas contains 15%. Accuracy of logs volume tables is unbiased.

No description available.

The objective of this thesis is to demonstrate, through legal and regulatory analysis, how three non-trade issues - SPS measures, trade in GMOs and food security concerns - might result in new modem barriers to trade that might diminish the gains of freer markets. If developed countries use non-trade concerns to justify more generous domestic support in a non-decoupled way, may do as much harm to international trade as the traditional trade policies instruments did in the past. Such undesired behavior from the developed world is possible due to the inability of current WTO norms to control these new problems. These three special issues must be addressed in future negotiations in order to modify developing countries' perception that the payoffs of trade liberalization are not advantageous for them. The continuance of this perception during current negotiations might lead to the collapse of the current trading system.