Nanocomposites have been receiving a lot the attention in the last decade from both industry and academia, since a small amount of nanofiller can significantly improve the materials properties. In the field of thermoplastics, polypropylene (PP) is one of the most used materials , due its easy processability, good balance of mechanical properties, and low cost. However, PP has certain shortcomings such as poor gas barrier and low thermal stability which limit its application. In order to be classified as nanocomposite the material needs to have at least one phase with one dimension less than 100nm. The properties achieved by nanocomposites will depend on the type of polymer, type of dispersed phase (filler), surface interaction between filler and polymer, and the production method. Nanofillers present many shapes and sizes, but they can be grouped in nanoparticles, nanotubes and nanoplates. Montmorillonite (MMT) is a clay that has been extensively studied to produce PP nanocomposites, due to its availability, high aspect ratio, high modulus and high cation exchange capacity, characteristics that result in composite with improved properties. Three different morphologies can be observed in PP/MMT nanocomposites: agglomerates (similar to the conventional composites); intercalated; or exfoliated. Among these morphologies, exfoliation is the most desirable and the hardest to be achieved in PP/MMT nanocomposites. Several methods have been used to produce PP nanocomposites. They can be grouped in three main groups: solution blending; melt processing; and in situ polymerization. In order to produce an exfoliated nanocomposite, some methods have assisted the exfoliation using supercritical fluids. Supercritical carbon dioxide is by far the most explored one. Polypropylene is a semi-crystalline polymer and its properties rely on amount of its crystallinity, which is related to its stereochemical configurations. Isotactic PP and syndiotactic PP result in a semi-crystalline polymer while atactic results in an amorphous polymer. Two catalyst systems can be used to produce isotactic PP: Metallocene and Ziegler-Natta (ZN). This research study was carried out in order to develop an appropriated process to produce PP/MMT nanocomposites with a high level of exfoliation using in situ polymerization assisted by supercritical propylene. The main idea is to use supercritical propylene to treat the montmorillonite before polymerization. In this process, the small molecules of propylene diffuse inside the clay galleries under supercritical conditions (high pressure and temperature) until reaching complete saturation. Once this saturation is reached the mixture of polypropylene and clay is catastrophically decompressed and fed into an autoclave reactor. The propylene polymerization reaction is them catalyzed by ZN catalyst. The pressure of the mixture of propylene-montmorillonite from the supercritical condition to the reactor autoclave decreased significantly, allowing propylene to expand and exfoliate the clay as it was fed in the reactor. Propylene in supercritical conditions was used in this works because it is the monomer for the subsequently polymerization and because its good properties at supercritical conditions. In order to evaluate the results the following methods were used: transmission electron microscopy (TEM) to investigate the nanoscale sample morphology and evaluate the clay exfoliation, X-ray diffraction (XRD) to determine interlamellar distance, d001, of the clay, differential scanning calorimetry (DSC) to determine the amount of crystallization of polymer and composite, thermogravimetric analysis (TGA) to determine composite clay content, scanning electron microscopy (SEM) to evaluate the morphology, and clay swelling test to evaluate the compatibility among various pairs clays-solvent. The first part of this work evaluated the interaction and swelling effects of different pairs of clay-solvent with or without sonication. This was necessary in order to choose the best clay to carry out the study. Four solvents with different polarity (chlorobenzene, toluene, cyclohexane and hexane) and eight clays (seven organically modified and one unmodified) were evaluated with or without sonication. Closite 15A and 93A presented the best results with different solvents and they were selected for further experiments. The experiments also showed that sonication improves the swelling of the clay. Initial screening of the polymerization reaction was carried out using two conditions: feeding supercritical propylene without clay and adding clay without the addition of supercritical fluid. The addition of supercritical propylene did not modify the morphology and properties of PP in comparison to the normal polymerization. The addition of Cloisite 15A or Cloisite 93A (pre-treated with toluene, not with supercritical propylene) produced nanocomposites. Although Cloisite 15A showed better results on the swelling tests, Cloisite 93A presented much better polymerization yield, therefore it was selected for further investigation using treatment with supercritical propylene. Cloisite93A was submitted to a treatment under four different supercritical propylene conditions (temperature and pressure) for thirty minutes. Each mixture was subsequently fed to the reactor through a catastrophic expansion inside an autoclave reactor running a propylene polymerization reaction. The results from XRD and TEM show a significant improvement on the exfoliation when treating the clay under supercritical propylene conditions followed by in situ polymerization, as compared to the in situ polymerization without treating the clay with supercritical propylene. In conclusion, the utilization of supercritical propylene has improved the dispersion of the clay at the nanoscale during the preparation of these nanocomposites by in situ polymerization.

The Agricultural Product Standards Act, 1990 (Act 119 of 1990) of South Africa provides for the standardisation of quality norms for agricultural produce (DAFF, 2018). The purpose of this research was to investigate the quality and compliance of fresh produce sold in the Tshwane metropolis with the Agricultural Product Standards Act of South Africa. A cross-sectional study survey was conducted in six regions of Tshwane metropolis, in which 200 fresh produce vendors were randomly selected from various vending sites to analyse their level of knowledge regarding the Act. Furthermore, different fruits and vegetables were sampled from different vending sites and inspected for compliance with the Act.The vast majority (89%) of respondents did not know of the existence of the Act. Quite a small proportion of fruit (51.5 %) and vegetable (56 %) retailers indicated that their agricultural fruit products have been monitored. A large proportion of fruits (from 86% to 99.8%) and vegetables (from 85.4% to 97.5%) met all the various quality requirements. In general a fair proportion of fruits (58%) and vegetables (65%) complied highly with the Act in terms of quality. There was a significant positive correlation between monitoring by officials and the level of compliance. The Department of Agriculture Forestry and Fisheries should make sure that farmers and street vendors of agricultural produce register their businesses and are trained on the interpretation of the provisions of the Agricultural Product Standards Act. This will allow them to be tracked and traced for proper development concerning matters related to the Act. / Life and Consumer Sciences / M.A. (Consumer Science)

Government, donors and NGOs in southern Niassa have been, after the 1992 peace agreement, extensively involved in agricultural development programmes to improve smallholders' food security. A study of the area and literature review revealed that many factors limited the benefits of agricultural market development programmes. Yet, opportunities in southern Niassa suggested that appropriately designed programmes could improve the standard of living of smallholders if these programmes were designed on a solid understanding of factors and strategies influencing agricultural market participation by smallholders. The main research hypothesis of this study was that: smallholders would participate in agricultural markets when their wealth status was high, when they had enough available household labour and when cash crops were profitable." Four main hypotheses were investigated: (i) factors and strategies identified through smallholder perceptions would provide local and time specific information on the constraints and solutions to smallholder market participation; (ii) wealth status and wealth-ranking factors were positively related to market participation where agriculture was the main economic activity as in southern Niassa; (iii) labour aspects such as crop labour requirements (CLR) could be negatively related, while available household labour (AHL) and the ratio AHL/CLR could be positively related to smallholders cultivation of cash crops and subsequent participation in agricultural markets; and (iv) aspects of profitability and indicators could be used to predict smallholder cash crop preferences. Data for this study were collected in Cuamba district of Mozambique from nine focus group discussions (FGDs) with community leaders, 287 household-head questionnaires and staff interviews during September 2002. Nine villages were randomly selected. The leaders' FGDs provided the criteria utilised to rank households according to wealth status and much of the qualitative information of this study. The wealth-ranking tool was used to identify and analyse the socio-economic factors that influenced smallholder market participation. A follow-up interview of managers of promoting institutions also provided greater insight on some aspects raised by smallholders. The study employed (i) descriptive statistics such as means and frequencies; (ii) correlation analysis and standard scores (iii) qualitative analysis was also used for some wealth-ranking, perceived labour demand and aspects of profitability influencing cash crop cultivation, preference and market participation based on information from FGD, farmers and staff; and (iv) simple mathematical expressions for analysis and interpretation of the research findings. This study relied on perceptions, knowledge and experience of smallholders, leaders and leaders of promoting institutions. Smallholder-suggested factors and strategies were in line with the limitations of socio-economic characteristics such as low effective household labour, particularly for females. These strategies included an improvement in outputs and inputs markets, agricultural services and credit at a subsidised prices or low interest rates. Other strategies for improving smallholders' participation in agricultural markets included promotion of profitable cash crops, household food security, provision of extension support services and information about cultivation and agricultural markets. However, smallholders did not identify some factors that have been acknowledged to influence agricultural market participation: ecological and natural resources, policies, institutional infrastructures and physical infrastructures. Smallholders also did not mention socio-economic factors (except household labour) as influencing their decisions to participate in agricultural markets in spite of the fact that researchers assume these factors in almost every study on smallholder market participation. The findings of this research confirmed that a wealth-ranking tool could be used to identify the socio-economic factors affecting smallholders' participation in agricultural markets. The identified wealth-ranking factors such as labour, livestock number, implements and bicycles significantly correlated with wealth status and subsequently to smallholder agricultural market participation. Conversely, household socio-economic characteristics not indicated as wealth-ranking factors such as age and gender related poorly to market participation. The wealth-ranking tool could also be used to identify strategies for improving smallholder participation in agricultural markets, and to evaluate an agricultural market development programme. The study found that, other factors being held constant, CLRs were negatively related to market participation. Weeding was the most labour intensive operation followed by harvesting, soil preparation, transportation, land clearing and seedling preparation. It also found that AHL and the ratio AHL/CLR were positive and significantly related to market participation. The ratio AHL/CLR together with household consumption requirements and yield were used to estimate the total area a household could cultivate, both for food crops for consumption and for cash crops; the proportion of farmers likely to participate in the market; and those unable to cultivate enough for consumption. The research also confirmed that profitability-related aspects correlated to cash crop preferences. Yield was the most important factor that influenced smallholders' preference for cash crops. It was also found that indicators incorporating more aspects of profitability correlated strongly with cash crop preferences. The correlation increased as more aspects were incorporated. A crop, such as tobacco, with a profit of more than twice the profit for food cash crops was preferred more than food cash crops. The indicators and underlying aspects of profitability were used to interpret the current and projected cash crop preference. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.

This thesis is an implicit farmland value study which explores the possible effects of agricultural commodity prices, interest rate and land reform issues on farmland values. The study examines the impacts of these fundamental factors (interest rates and returns to farmland as determined by crop prices) on sugar cane farmland values, maize farmland values, on deciduous fruit (apples and pears) farmland values, and on aggregate South African farmland values. Expectations are that land reform influences the demand for farmland. Since farmland prices are demand driven, changes in the demand for farmland (as influenced by land reform issues) may result in changes in farmland prices. The study thus seeks to empirically examine, to a larger extent, the long-run influence of endogenous factors on farmland prices. Causes of cyclical behaviour in farmland prices are also examined. The study draws on cross-sectional and time series studies of previous research on farmland values. The maximum likelihood Johansen (1991) procedure of cointegration is used to estimate the relationship between fundamental factors and farmland values. The logit model is used to estimate the influence of land reform on the demand for farmland, hence farmland prices. Unit root and the Johansen cointegration test results proved that long-run relationships exist between farmland values and returns to farmland; the use of cointegration methods was thus recommended. Long-run changes in farmland prices are caused by fundamental factors. Short-run variations in farmland prices are caused by exogenous factors that affect net farm income and this lead to boom-bust cycles in farmland values. / Thesis (M.Comm.) - University of KwaZulu-Natal, Pietermaritzburg, 2009.

Thesis (M.A. Agricultural Management (Agricultural Economics)) -- University of Limpopo -- 2020 / Potato is the most important vegetable crop in South Africa in terms of contribution to the gross value of agricultural production, export earnings and contribution to food supply base and food security in the SACU region. Despite the importance of this commodity, very little is known about the nature of price transmission between different levels of potato value chain in South Africa. The study aims to determine the nature of price transmission in the South African potato market. The objectives of the study are to investigate the existence of long-run equilibrium relationship between producer, wholesale and retail prices; to determine characteristics of the relationship; and to determine the direction of price causality. The study made use of Error Correction Model and Granger Causality test. The Empirical results reveal the existence of price asymmetry in the South African potato value chain. Furthermore, the results show that retail prices are more responsive producer price increases than they are to producer price declines. The Granger causality test shows that prices in potato value chain are determined mainly at the wholesale level (i.e. at the National Fresh Produce Markets). The study recommends further research focusing on price transmission for other basic food commodities and that the government retains and strengthens the existing food price monitoring system. / Department of Agriculture, Forestry and Fisheries

This thesis presents methods for simplifying the use of multi-agent systems in Plug & Produce. The demand for customized products and low volume production is constantly increasing. The industry has for many years used dedicated manufacturing systems where it is difficult and expensive to adapt to new product designs. Instead, factories are forced to use human workers for certain tasks that demand high flexibility and rapid adaption for new product designs. Several solutions have been proposed over the years to create highly flexible automation systems that automatically handles rapid adaption for new products. A concept called Plug & Produce aims at creating a system where resources and parts can be added in minutes rather than days in dedicated systems. One promising solution for implementing Plug & Produce is the distributed approach called multi-agent systems, where each resource and part get its own controller that communicates with each other to reach manufacturing goals. The idea is that the system automatically handles the adaption for new products. However, still today the use of such systems is extremely limited in the industry. One reason is the lack of mature multi-agent systems that are easy to use and that hides the complexity of the underlying agent system from the users. This is a huge problem since these systems tend to be more complex than traditional approaches. Thus, this thesis focuses on simplifying the use of multi-agent systems by proposing various methods for bringing the multi-agent technology for Plug & Produce closer to the industry. / Denna avhandling presenterar metoder för att förenkla användningen av multiagent-system för Plug & Produce. Efterfrågan på kundanpassade produkter och lågvolymproduktion ökar ständigt. Industrin har under många år använt sig avdedikerade tillverkningssystem som gör det både svårt och dyrt att anpassa sig till nya produktdesigner. Istället tvingas fabriker att antälla onödigt många operatörer för vissa arbetsuppgifter där det krävs hög flexibilitet och snabb anpassning till nya produktdesigner. Flera lösningar har föreslagits under åren för att skapa flexibla automatiseringssystem som automatiskt hanterar snabb omställning till nya produkter. Ett koncept som heter Plug & Produce handlar om att skapa system där nya typer av resurser och produkter kan kopplas in i systemet på ett fåtal minuter snarare än dagar i traditionella system. För att implementera Plug & Produce kan multi-agent-system användas, där varje resurs och produkt får sin egen styrning. Agenterna kan sedan kommunicera med varandra för att nå de mål som satts upp för tillverkningen av produkterna. Tanken är att systemet automatiskt hanterar anpassningen till nya produkter. Idag är dock användningen av sådana system extremt begränsad i industrin. En av anledningarna är avsaknaden av mogna multi-agent-system som är lätta att använda och där komplexiteten hos det underliggande agensystemet kan döljas från användaren. Detta är ett stort problem eftersom multi-agent-system tenderar att vara mer komplexa än traditionella system. Därför fokuserar denna avhandling på att förenkla användningen av multi-agent-system genom att föreslå olika metoder som kan underlätta användandet av multi-agent-tekniken för Plug & Produce i industrin.

No description available.

Adaptations to a fluctuating market and intensified customer demands for unique products are a challenge for manufacturers. Manual manufacturing is still the most flexible, nevertheless, automation ensures stable quality, minimizes wear and tear of the operators, and contributes to a safer and better working environment as the distance between the operator and the process can be increased and screened off. Hence, the manufacturing industry is searching for human-centric automation solutions that are flexible enough to handle these challenges. Conventional automation is tailored for one or a few similar variants of products, in addition, increased flexibility implies increased complexity to handle. This licentiate thesis demonstrates a flexible Plug &amp; Produce automated manufacturing concept where the complexity is redirected to focus on the products and manufacturing processes by utilizing artificial intelligence. Together with digital threads that connect the product design to automatic manufacturing that enables manufacturing companies to manage new production scenarios with their in-house knowledge. Data is picked directly from the computer-based design of the products and process knowledge that normally exists within the manufacturing company is added through graphical user interfaces. The graphical configuration tools visualize the flow of sequential and parallel manufacturing operations together with process-bound information. Plug &amp; Produce relies on pluggable process modules with re-cyclical manufacturing resources that can be plugged in and out as needed. As an example, a module with a robot can be plugged in to help an existing robot and thereby balance the production capacity. In Plug &amp; Produce resources start working and cooperate with other resources automatically when they are plugged in. To achieve this, the resources are provided with distributed artificial intelligence together with intelligent products that know how to be finalized. In this concept, everything is digitally configurable by the in-house knowledge of the manufacturing companies. A Plug &amp; Produce test bed was built to verify the concept in cooperation with industrial representatives. / Denna licentiatavhandling påvisar ett koncept för att öka flexibiliteten och samtidigt rikta om komplexiteten i automatiserade produktionssystem hos tillverkande företag på ett sätt så att deras interna personal på egen hand kan ställa om tillverkningen mot nya produkter. Anpassningar till marknadens fluktuationer och efterfrågan av nya unika produkter är en ständigt pågående process. Alltmer av produktionen flyttas tillbaka till Sverige och övriga Europa vilket ökar efterfrågan på flexibel och omställbar automation. Automation håller nere prisnivån då arbetskraften är dyr, säkerhetsställer jämn kvalité, minimerar förslitningsskador på de anställda och bidrar till säkrare och trevligare arbetsmiljö då distansen mellan operatör och process kan ökas och avskärmas. Produktion som flyttas till hemmamarknaden från låglöneländer ersätter ofta högflexibel och anpassningsbar manuell tillverkning vilket är en stor utmaning för industrin. Ett Plug &amp; Produce koncept för automatiserad tillverkning utvecklas och beskrivs i denna avhandling där automationen enkelt kan ställas om av den interna personalen och anpassas till nya produkter. Omställning med hjälp egen personal möjliggörs genom att så mycket information som möjligt utvinns från produktens datorbaserade design. Processkunskap som normalt besitts inom det tillverkande företaget adderas därtill med hjälp av grafiska användarinterface som visar flödet av tillverkningsoperationer tillsammans med processpecifika uppgifter såsom mått, bearbetningshastigheter, temperaturer och färg. Plug &amp; Produce system är uppbyggda kring processmoduler med tillverkningsresurser som kan pluggas in och ut efter behov. Till exempel kan en modul med en robot pluggas in för att avlasta befintlig robot och därmed öka produktionshastigheten. Specialdesignade resurser kan pluggas in för att öka effektiviteten och minimera energikonsumtionen. För att den inpluggade processmodulen självmant skall börja jobba och samarbeta med de andra modulerna är den försedd med egen lokal artificiell intelligens. Dessa processmoduler kan tack vare sin intelligens pluggas in i olika Plug &amp; Produce system och är därmed återvinningsbara i nya system. Intelligensen kan vara lokalt placerad i en dator på resursen eller i datormolnet kopplat till resursen. På samma sätt kan produkterna förses med intelligens och kallas då för smarta produkter. Dessa produkter har som mål att bli färdigproducerade genom delmål i form av tillverkningsoperationer. Denna intelligens förses med kunskap och erfarenheter av personalen inom det tillverkande företaget genom användarvänliga interface. När användarvänligheten Plug &amp; Produce testbädd har byggts upp tillsammans med representanter frånprefabricerade trähusindustrin. Tillverkning av prefabricerade trähus är i idag ihög grad manuell då existerande automationslösningar inte är flexibla nog eftersom husen är i hög grad är kundanpassade. Arbetet som beskrivs i denna avhandling gynnar trähusindustrin och därmed klimatet då trä binder kol för en lång tid framåt. / <p>Paper A is not included in the digital licentiate thesis due to copyright . </p>

Thesis (MBA)--Stellenbosch University, 2010. / ENGLISH ABSTRACT: The Marketing of Agricultural Products Act 47 (No 47 if 1996) led to the dilution of single-channel marketing in the agricultural sector. Hence, marketing boards such as the Maize Board were dismantled. New legislation forced agricultural businesses to adapt to the changing environment and turn co-operatives into companies. The new developments also implied that these companies had to reposition themselves to be more competitive in a deregulated market. MGK Group Operating Company Pty (Ltd) is a prominent role player in the agricultural sector in the North West, Limpopo, Mpumalanga and Gauteng provinces in South Africa. Unexpected growth in the company as well as the deregulation of the industry necessitated a bona fide marketing communication department to manage and coordinate all advertising, promotional and communication efforts. After establishing this department all marketing communication efforts were still fragmented. Consequently, the cost-effectiveness of this department was questioned by the company’s board of directors. The MGK Group’s the board of directors received a mandate to follow a holistic approach and formulate a strategy to enhance the impact of its marketing communication efforts while cutting costs. This study sets out to establish whether the implementation of an integrated marketing communication programme can ultimately satisfy the board of directors’ requirements for marketing communication that is more accountable, effective and efficient. This study starts with a literature review of the practices and theories underpinning integrated marketing communication. This is followed by testing the applicability of the various approaches and methodologies in the MGK Group environment. The study is concluded with the formulation of a marketing communication strategy and recommendations based on the needs of the MGK Group. / AFRIKAANSE OPSOMMING: Die Wet op die Bemarking van Landbouprodukte (Nr 47 van 1996) het eenkanaal-bemarking in die sektor verwater. Dit het daartoe gelei dat bemarkingsrade soos die Mielieraad afgeskaf is. Nuwe wetgewing het landbou-ondernemings genoop om in 'n veranderende sake-omgewing aan te pas en koöperasies in maatskappye te omskep. Die aanpassing het ook beteken dat maatskappye hulself in 'n meer mededingende en gedereguleerde mark moes herposisioneer. Die MGK Groep Bedryfsmaatskappy Edms Bpk is 'n vooraanstaande rolspeler in die landbousektor in die volgende provinsies in Suid-Afrika: Noordwes, Limpopo, Mpumalanga en Gauteng. Onverwagse groei asook die deregulering van die bedryf het meegebring dat dié maatskappy 'n bona fide bemarkingskommunikasie-afdeling nodig gehad het om alle reklame-, promosie- en kommunikasie-aktiwiteite te bestuur en te koördineer. Nadat die afdeling op die been gebring is, het bemarkingskommunikasie-aktiwiteite steeds los van mekaar gestaan. Dit het daartoe gelei dat die kostedoeltreffendheid van die funksie op divisievlak bevraagteken is. Die direksie van die MGK Groep het 'n mandaat gehad om 'n holistiese benadering te volg en 'n strategie te formuleer om die impak sowel as die kostedoeltreffendheid van die maatskappy se bemarkingskommunikasie te verbeter. Hierdie studie poog om vas te stel of die implementering van 'n geïntegreerde bemarkingskommunikasieplan uiteindelik in die direksie van die MGK Groep se behoefte aan verantwoordbare, doeltreffende en doelmatige bemarkingskommunikasie kan voorsien. Die studie begin met 'n literatuuroorsig van die praktyk en teorie van geïntegreerde bemarkingskommunikasie. Daarna word die toepaslikheid van die onderskeie benaderings en metodologieë ten opsigte van die MGK Groep getoets. Die studie word afgesluit met 'n bemarkingskommunikasiestrategie en aanbevelings wat op die behoeftes van die MGK Groep gegrond is.

This work introduces technology for rapid pathogen detection using handheld optical immunoassay and wire-guided droplet PCR systems. There have been a number of cases of foodborne or waterborne illness among humans that are caused by pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, Influenza A H1N1, and the norovirus. The current practices to detect such pathogenic agents are: cell/viral culturing, immunoassays, or polymerase chain reactions (PCRs). These methods are essentially laboratory-based methods that are not at all real-time and thus unavailable for early-monitoring of such pathogens. They are also very difficult to be implemented in field, preventing early detection opportunities. This dissertation is divided into three papers that present methodologies towards the expeditious detections of infectious pathogens and the miniaturization of these detection systems towards field-deployable and point-of-care applications. Specifically, the work presented focuses on two methodologies: (1) light scatter detection using immunoagglutination assays with optimized Mie light scatter parameters in a real biological matrix consisting of plant tissue, and (2) wire-guided droplet manipulations for rapid and improved sample analysis, preparation, and PCR thermocycling. Both of these methods carry a collective objective towards providing high impact technologies for addressing the issues of food-related outbreaks and overall public safety. In the first paper, the direct and sensitive detection of foodborne pathogens from fresh produce samples was accomplished using a handheld lab-on-a-chip device, requiring little to no sample processing and enrichment steps for a near-real-time detection and truly field-deployable device. The detection of Escherichia coli K12 and O157:H7 in iceberg lettuce was achieved utilizing optimized Mie light scatter parameters with a latex particle immunoagglutination assay. The system exhibited good sensitivity, with a limit of detection of 10 CFU mL⁻¹ and an assay time of <6 min. Minimal pretreatment with no detrimental effects on assay sensitivity and reproducibility was accomplished with a simple and cost-effective KimWipes filter and disposable syringe. Mie simulations were used to determine the optimal parameters (particle size d, wavelength λ, and scatter angle θ) for the assay that maximize light scatter intensity of agglutinated latex microparticles and minimize light scatter intensity of the tissue fragments of iceberg lettuce, which were experimentally validated. This introduces a powerful method for detecting foodborne pathogens in fresh produce and other potential sample matrices. The integration of a multi-channel microfluidic chip allowed for differential detection of the agglutinated particles in the presence of the antigen, revealing a true field-deployable detection system with decreased assay time and improved robustness over comparable benchtop systems. In the second paper, we demonstrate a novel method of wire-guided droplet manipulations towards very quick RT-PCR. Because typical RT-PCR assays take about 1–2 h for thermocycling, there is a growing need to further speed up the thermocycling to less than 30 min. Additionally, the PCR assay system should be made portable as a point- of-care detection tool. Rapid movements of droplets (immersed in oil) over three different temperature zones make very quick PCR possible, as heating/cooling will be made by convective heat transfer, whose heat transfer coefficients are much higher than that of conduction, the latter of which is used in most conventional PCR systems. A 30-cycle PCR of a 160 bp gene sequence amplified from 2009 H1N1 influenza A (human origin) was successfully demonstrates in 6 min and 50 sec for a very large 10 μL droplet (with additional 4 min for reverse transcription). The proposed system has a potential to become a rapid, portable, point-of-care tool for detecting influenza A. In the third paper, a wire-guided CNC apparatus was used to perform droplet centrifugation, DNA extraction, and VQ-PCR thermocycling on a single superhydrophobic surface measuring 25 mm by 55 mm and a multi-chambered PCB heater. This methodology exhibited no limitations on the complexity and configuration of procedures that it can perform, making it versatile and far-reaching in its applications. The only modification required for adding or implementing changes for a new protocol is through simple pre-defined programming. The highly adaptive and flexible system was used to execute easily pre-programmed droplet movements and manipulations for the rapid detection of Escherichia coli from PCR detection. Serial dilutions were performed to simulate a diluted field sample with a high level of accuracy. Centrifugation of the diluted sample containing E. coli demonstrated a novel approach to sample pre-treatment. Furthermore, the extraction of DNA from the sample droplet containing E. coli was also performed on the same superhydrophobic surface as the previous 2 steps, requiring less than 10 min. Following extraction, the genetic material was amplified using wire-guided droplet PCR thermocycling, successfully completing 30 cycles of Peptidase D (a long 1500 bp sequence) in 10 min. The droplet centrifugation process was determined to greatly improve the positive band intensity over the non-centrifuged sample. Thus, this work demonstrates the adaptability of the system to replace many common laboratory tasks on a single platform (through re-programmability), in rapid succession (using droplets), and with a high level of accuracy and automation.