Thesis (D. Sc.)--University of Adelaide, 1988. / Collection of previously published articles. Includes Allozyme electrophoresis / B.J. Richardson, P.R. Baverstock and M. Adams (1986). Includes bibliographical references.

Thesis (Ph. D.)--University of Adelaide, Dept. of Biochemistry, 1992. / Cover title: Novel approaches to protein purification and analysis: counter-current electrophoretic filtering: spectral enhancement. Spine title: Protein purification and analysis. Includes bibliographical references.

Cross-over electrophoresis, an immunological method for analyzing blood residues on archaeological artifacts, is tested. Artifacts from three sites were utilized in the testing of this methodology. The sites are the Dietz site in south-central Oregon (282 artifacts), Konemehu in northern California (48 artifacts tested for Winthrop Associates), and Chimney Shelter in southwestern Oregon (3 artifacts from the Umpqua National Forest).

Thesis (M.Sc.)--Memorial University of Newfoundland, 1987. / Typescript. Bibliography : leaves 91-103. Also available online.

A Distrofia Muscular de Duchenne (DMD) é uma doença genética recessiva e hereditária, ligada ao cromossomo X, caracterizada pela ausência ou insuficiência da proteína distrofina no sarcolema das fibras musculares. É uma doença muscular progressiva, que afeta um em cada 3.500 meninos. Atualmente, os cães da raça Golden Retriever, portadores da distrofia muscular do Golden Retriever (GRMD), que exibem mudanças musculares, patogenia, bem como o fenótipo, comparáveis aos vistos nos casos de DMD, vêm sendo considerados o melhor modelo animal para o estudo da DMD. Nos GRMDs a fraqueza muscular inicia-se a partir do segundo mês de vida e é progressiva, portanto, a expectativa de vida destes cães é muito reduzida. Histologicamente, os músculos mostram necrose, fibrose e regeneração. As manifestações patológicas da GRMD já se iniciam na vida intra-uterina com o desenvolvimento de lesões nos músculos da língua. A hipertrofia da língua está associada com disfunção da faringe e do esôfago, o que resulta em disfagia,regurgitação e sialorréia. O objetivo desse estudo foi avaliar no modelo animal, utilizando cães golden retrievers normais e afetados pela distrofia muscular de duchenne, a proteína na urina e o peso molecular das proteínas urinárias, além de acompanhar a bioquímica sérica. Durante quatro meses seis cães machos com distrofia muscular e idade entre dois e seis anos tiveram amostras de urina coletadas a cada duas semanas e amostras de sangue coletadas uma vez por mês, e três cães normais foram avaliados para estabelecer uma comparação. Nas amostras sanguíneas foram realizados exames para avaliar a função renal e a urina foi usada para a mensuração de proteína, creatinina e a realização da eletroforese. Não foi encontrado indício de lesão renal nos exames realizados nas amostras de sangue e também na relação proteína:creatinina nas amostras de urina. Na análise das eletroforeses, foi encontrado diferenças na quantidade de bandas protéicas apresentadas entre os animais normais e os cães com distrofia muscular. Os cães com distrofia apresentam uma maior distribuição de bandas de alto peso molecular que não são encontradas nos cães normais, além de terem ainda uma maior quantidade de proteínas de baixo peso molecular. Apesar de se manterem dentro dos parâmetros estabelecidos para lesão renal, os cães com distrofia, tem bandas protéicas que não são vistas em animais normais e que mantém a integridade do parênquima renal. / Duchennes Muscular Dystrophy (DMD) is a lethal childhood disease, a X-linked recessive disorder, caused by mutations of the dystrophyn gene, a protein that has a vital role in maintaining muscle structure and function. DMD is a progressive muscular disease, which affects 1:3500 born boys. At this moment, the dogs with Golden Retriever Muscular Dystrophy (GRMD), are the best animal model to study DMD. GRMD dog exhibits muscle changes, pathogenesis and phenotype comparable with the DMD boys. In GRMDs, the muscular weakness beginning with 60 days of life and this is progressive. Histologically, the muscles show necrosis, fibrosis, degeneration and regeneration. The pathogenesis manifests in utero with the development of lingual muscle lesions. The tongue hypertrophy is associated with pharyngeal and esophageal dysfunction, and results in dysphagia, regurgitation and drooling. The aim of this research was investigates in normal dogs and in dogs affected by the Duchene muscular dystrophy the protein in the total urinary proteins and their separation with SDS-PAGE. During four months, six male dogs between two and six years were evaluated. The blood was examined once a month and the urine twice a month, three normal dogs were examined to establish a comparison between both. In blood samples were carried out tests to evaluate renal function and urine was used for the measurement of protein, creatinine and for electrophoresis. We found no evidence of kidney damage in tests performed on blood samples and also in the protein: creatinine ratio in urine samples. In the analysis of electrophoresis, we found differences in the amount of protein bands presented between normal dogs and dogs with muscular dystrophy. GRMD dogs have a wider distribution of bands with high molecular weight, which are not found in normal dogs. In addition they have a greater amount of protein with low molecular weight. While staying within the parameters established for renal injury, the dogs with dystrophy, has protein bands not seen in normal animals that maintains the integrity of the renal parenchyma.

Collection of previously published articles / Includes Allozyme electrophoresis / B.J. Richardson, P.R. Baverstock and M. Adams (1986) / Includes bibliographies / 2 v. : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (D. Sc.)--University of Adelaide, 1988

No description available.

Die vorliegende Arbeit beschäftigte sich mit zwei Methoden zur Diagnostik der Aspergillose bei Greifvögeln, um neue Erkenntnisse über die Aussagekraft dieser nicht invasiven Diagnostika zu gewinnen. In der ersten Studie wurden bei ausschließlich Aspergillose-positiven Falken (Falco spp.) (n = 110) spezifische Röntgenzeichen an digital erstellten Röntgenbildern systematisch ermittelt und mit den typischen Röntgenzeichen von Papageien mit Erkrankungen des unteren Respirationstrakts verglichen. In der zweiten Studie wurden gesunde (n = 73) und an Aspergillose erkrankte (n = 32) Jagdfalken (Falco spp.) mittels Serumelektrophorese untersucht, Referenzwerte für die gesunden Falken erstellt und mit den Werten der erkrankten Falken verglichen. In beiden Studien stammten die Tiere aus dem Patientengut derselben Klinik. Bei der Auswertung von Röntgenbildern Aspergillose-positiver Falken wurden hauptsächlich subtile Röntgenzeichen beschrieben. Von den 110 Tieren waren 29 (26,4 %) radiologisch vollkommen unauffällig. Die am häufigsten beschriebenen Befunde waren inhomogene Verschattungen des Lungenfeldes (38,2 % laterolateral [ll]) und strichförmige Verschattungen der kaudalen Lungengrenze (30,0 % ll) sowie inhomogene (34,5 % ll; 29,1 % ventrodorsal [vd]) und streifige (26,4 % ll) Verschattungen der Luftsäcke, aber auch eine schlechte Abgrenzbarkeit des Herzschattens in der laterolateralen Projektion (42,7 %). Im Vergleich zu an Papageien mittels konventioneller Projektionsradiographie durchgeführten Studien war der Anteil an subtilen Röntgenzeichen geringer und der Anteil an massiven Röntgenzeichen größer. Verglichen mit Referenzwerten diverser Greifvogelspezies aus der Literatur zeigten die Referenzwerte der gesunden Falken dieser Studie unter Verwendung des hochauflösenden Elektrophoresesystems SAS 1 unit (Helena, Saint Leu La Forest, Frankreich) relativ niedrige Gesamtproteinwerte und relativ hohe Präalbuminwerte auf. Bei den 32 Serumproben der an Aspergillose erkrankten Falken ließ sich im Gegensatz zu den 73 Serumproben der gesunden Falken ein signifikant erniedrigter Totalalbuminwert (Albumin + Präalbumin) sowie ein hoch signifikant erniedrigter Präalbuminwert mittels Serumelektrophorese feststellen. Obwohl die Falken meist schon in frühen Krankheitsstadien vorgestellt wurden und die Diagnostik in diesen Stadien besonders schwierig ist, konnten mit beiden Untersuchungsmethoden von gesunden Tieren differierende Befunde erhoben werden. Diese in Zusammenhang mit Aspergillose erhobenen Befunde wichen jedoch teilweise deutlich von den in der Literatur beschriebenen „typischen“ Befunden bei an Aspergillose erkrankten Vögeln ab. Dies kann damit erklärt werden, dass die meisten vergleichbaren Studien an als Heimtiere gehaltenen Papageien oder gefangen gehaltenen Zoovögeln (von Falken abweichende Haltungsform, Anatomie und Physiologie sowie Leistungsniveau) und mit unterschiedlicher Technik (digitale versus konventionelle Projektionsradiographie, unterschiedliche Elektrophoresesysteme und Verwendung von Serum anstelle von Plasma) durchgeführt wurden. Die digitale Projektionsradiographie kann aufgrund ihrer schonenden, einfachen und schnellen Durchführbarkeit sowohl den Vogelmedizin spezialisierten Institutionen als auch den Kleintierpraktikern uneingeschränkt empfohlen werden. Die Proteinelektrophorese kann bis zum heutigen Zeitpunkt nur bedingt für den Praktiker, wohl aber für spezialisierte Institutionen bei Beachtung aller Besonderheiten als zusätzliches Diagnostikum empfohlen werden. / The present study concentrates on two methods for diagnosing birds of prey with aspergillosis with the intent to increase the knowledge of the validity of these non-invasive diagnostic methods. In the first study, specific radiographic signs of digitally created radiographs of falcons (Falco spp.) which were exclusively positive for aspergillosis (n = 110) were systematically analyzed and compared to the typical radiographic signs of parrots with diseases of the lower respiratory tract. In the second study, healthy falcons (n = 73) and falcons affected with aspergillosis (n = 32) (Falco spp.) were examined by using serum protein electrophoresis in order to create reference values for healthy falcons and compare them with the values of the affected falcons. In both studies, the animals were patients of the same clinic. While evaluating the radiographs of the falcons with aspergillosis, mainly subtle radiographic signs were described. Radiographically within normal limits were 29 (26.4%) of the 110 animals. The most commonly reported findings were inhomogeneous increased radiodensity of the lung area (38.2% laterolateral [ll]), line-shaped shadowings of the caudal lung border (II 30.0%) as well as an inhomogeneous (34.5% ll, 29.1% ventrodorsal [vd]) and streaky (26.4% II) radiodensity of the air sacs, but also a poor delineation of the cardiac silhouette in the laterolateral projection (42.7%). Compared to studies performed on parrots by conventional radiography, the portion of subtle radiographic signs was lower and the portion of severe signs was higher. Compared to reference values of various raptor species from the literature, this study, which made use of the high-resolution electrophoresis SAS 1 unit (Helena, Saint Leu La Forest, France), revealed relatively low values for total proteins and relatively high values for prealbumin in the reference values of the healthy falcons. The 32 serum samples of the falcons suffering from aspergillosis showed a significantly reduced total albumin (albumin + prealbumin) level and a highly significantly reduced prealbumin level compared to the 73 serum samples of healthy falcons. Although the falcons were for the most part already brought to the clinic in one of the early stages of the disease, when diagnosing aspergillosis is particularly difficult, both examination methods revealed different results for the healthy and diseased animals. However, the findings related to aspergillosis were in some cases significantly different from those described in the literature as the \"typical\" findings in birds suffering from aspergillosis. This can be explained by the fact that most of the comparable studies were conducted with parrots held as pets or with captive zoo birds (when husbandry, anatomy and physiology, as well as performance level are different from falcons) and with a different technique (digital versus conventional radiography, different electrophoresis systems and the use of serum instead of plasma). The digital radiography can be fully recommended for specialized medical institutions for avian medicine as well as for small animal practitioners because of its easy, rapid and gentle feasibility. To date, the protein electrophoresis can only be recommended with restrictions for practitioners, however for specialized institutions, it can be useful as additional diagnostic tool if all its specific features are taken into account.

Cover title: Novel approaches to protein purification and analysis: counter-current electrophoretic filtering: spectral enhancement / Spine title: Protein purification and analysis / Includes bibliographical references / 145 p. : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Summary: New inventions are described in the areas of electrophoresis and spectroscopy / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1992

Orientadora: Marileda Bonafim Carvalho / Banca: Julieta Rodini Engracia Moraes / Banca: Aguemi Kohayagawa / Resumo: Para este estudo foram avaliados 20 cães adultos sadios (10 machos e 10 fêmeas) e 24 cães adultos (16 machos e 8 fêmeas) com insuficiência renal crônica (IRC), com o objetivo de estudar a proteinúria. Foram empregadas técnicas para determinação quantitativa de proteína da urina e para a separação de suas frações por meio de SDS-PAGE. As quantidades de proteínas presentes na urina de cães sadios foram pequenas, mas o estudo dos perfis eletroforéticos mostrou haver diferenças entre machos e fêmeas. Apesar da ampla variação das concentrações de proteínas entre os animais, o grupo dos portadores de IRC apresentou proteinúria intensa. Os resultados dos portadores de IRC foram agrupados de acordo com o sexo ou em função do tipo de lesão renal predominante, para comparação dos eletroforetogramas. As análises revelaram que os perfis eletroforéticos seguem um padrão para os machos e outro pra as fêmeas. Ainda, o padrão o perfil eletroforético observado quando há predomínio de lesões glomerulares difere do observado quando há predomínio de lesões túbulo-intersticiais. / Abstract: This trial was conducted in 20 adult health dogs (10 male and 10 female) and in 24 adult dogs (16 male and 8 female) in chronic renal failure (CRF), in order to study urinary protein loss. Determination of total urinary proteins and their separation with SDS-PAGE were employed. The results show that CRF dogs had higher proteinuria when compared to controls and the amount of detected protein had large difference among them. In the CRF there are different patterns of protein bands occurrence and distribution according to the patient sex and predominant renal lesion. The difference between protein bands occurrence of male and female already exists in normal dogs. / Mestre