Via Negativa is my response to a linear, rational, Western framework of expressing and experiencing religion, as well as of reading. This project represents a shift away from Protestant Reformed thinking. It has meant an embrace of the unknown, a celebration of darkness, and an exploration of ancient religious texts to find meaning. This essay describes formative experiences and past artwork leading up to Via Negativa, as well as a description of the project itself.

Nanomaterials are widely used in many different products, such as electronics, cosmetics, industrial goods, biomedical uses, and other material applications. The heavy emission of nanomaterials into the environment has motived increasing concern regarding the effects on ecosystems, food chains, and, human health. Plants can tolerate a certain amount of natural nanomaterials, but large amounts of ENMs released from a variety of industries could be toxic to plants and possibly threaten the ecosystem. Employing phytoremediation as a contamination treatment method may show promise. However a pre-requisite to successful treatment is a better understanding of the behavior and effects of nanomaterials within plant systems. This study is designed to investigate the uptake, translocation, bioavailability, and toxicity of gold nanorods in maize plants. Maize is an important food and feed crop that can be used to understand the potential hazardous effects of nanoparticle uptake and distribution in the food chain. The findings could be an important contribution to the fields of phytoremediation, agri-nanotechnology, and nanoparticle toxicity on plants. In the first experiment, hydroponically grown maize seedlings were exposed to similar doses of commercial non-coated gold nanorods in three sizes, 10x34 nm, 20x75 nm, and 40x96 nm. The three nanorod species were suspended in solutions at concentrations of 350 mg/l, 5.8 mg/l, and 14 mg/l, respectively. Maize plants were exposed to all three solutions resulting in considerably lower transpiration and wet biomass than control plants. Likewise, dry biomass was reduced, but the effect is less pronounced than that of transpiration and wet biomass. The reduced transpiration and water content, which eventually proved fatal to exposed plants, were most likely a result of toxic effect of gold nanorod, which appeared to physically hinder the root system. TEM images proved that maize plants can uptake gold particles and accumulate them in root and leaf cells. However, the translocation factor of gold nanorods from root to leaf was very low in this experiment. In the second experiment, maize seedlings were exposed to different (lower) concentrations of gold nanorods measured at 4.5x10-3 mg/l, 0.45 mg/l, and 2.25 mg/l for 10 days. Transpiration and biomass measurements demonstrated that the higher concentration of gold nanorods caused lower water uptake and growth, but lower concentrations did not show a significant toxic effect. According to ICP-MS results, root systems of the exposed plants were surrounded by high concentrations of sorbed nanorods, which physically interfered with uptake pathways and, thus, inhibited plant growth and nutritional uptake.

Menopause is a biological change that affects the aging woman at some point in her life. Hormone replacement therapy (HRT) has been a primary medical intervention for decades, and this study explores how HRT products are marketed to women experiencing menopause through direct-to-consumer (DTC) drug ads. Through a qualitative analysis of DTC ads and interviews with women experiencing menopause symptoms, this research investigated their perspective on HRT drug ads to understand if women respond to this type of advertising. Women’s understanding and experiences concerning menopause are influenced by a number of factors and can vary depending on the meanings that are associated with menopause. In U.S. culture, physical appearance is emphasized above other characteristics, so menopause and other signs of aging chge the beauty ideal. Media portrayals of women too often value youth and ideal beauty, with direct-to-consumer (DTC) ads reinforcing this notion by emphasizing how women can remain young, fight the signs of aging, and maintain their vitality by using HRT products. Women also feel conflicted about their bodies as they age because of these dominant standards that can then lead to negative body image. Social comparisons are an inherent process guiding behavior and experiences that affect how people understand themselves (Corcoran, Crusius, & Mussweiler, 2011). People look at others and to media images of others, relating that information to themselves as a way to measure what they are and aren’t capable of. When advertisements construct menopause as a deficiency that women need to treat with medications, women compare themselves to mediated images as they try to understand their menopause experience. Ads analyzed for this study presented messages that women need medication to maintain healthy activities during and after menopause. Most of the ads focused on painful sex that can happen with menopause but nearly all of the participants agreed that these ads did not relate to their experiences. This research found that women don’t believe menopause is a disease to be treated but if medications are used, it should be for the shortest time possible and only if the symptoms drastically interfere with a woman’s quality of life. Through these interviews with menopausal women and analysis of HRT ads, this study adds to limited current research on DTC ads for hormone replacement therapies and menopause.

By the year 2020, the nanotechnology market is expected to be three trillion dollars. With a quasi-exponential increase in consumer products, which contain nanomaterials, there is likely to be an equal increase in nanoparticles entering the environment. As a result, it is imperative to fully understand the relationship between nanomaterials and the food chain, including plants. In this study, the relationship between gold nanomaterials and the edible food crop, Helianthus annuus was investigated. First, an attempt to inhibit the uptake of nanoparticles into the roots of H. annuus was investigated by decreasing temperature. Second, the interactions between citrate-stabilized 20 nm diameter Au nanoparticles and sunflower seedlings were explored by exposing sunflower to a range of concentrations (3.0-40.0 mg/L). Nanoparticle sorption to roots was estimated using a linear isotherm with a distribution coefficient, Kd. Finally, sunflowers were exposed to 20 nm Au nanoparticles and 25x69 nm CTAB-stabilized Au nanorods. Results showed there was no change in biomass growth and transpiration between sunflowers that were exposed to nanoparticles and the unexposed controls. Thus Au gold nanoparticles (20 nm) were shown to have no phytostimulatory or phytotoxic effect on sunflower seedlings during eight to ten day exposure experiments. However, 25x69 nm gold nanorods were phytotoxic to sunflowers at 6.0 mg/L, indicating a potential charge or chemical effect of the surface coating of the nanorods compared to the spherical gold nanoparticles.

The parallel actin filament bundling protein Fascin is a critical protein in both disease and development. Overexpression of Fascin is linked to increased aggressiveness in a number of cancer types, including breast and colon carcinomas. Importantly, Fascin is not normally expressed in adult epithelial cells from which many of these cancers arise. Therefore, Fascin is increasingly cited as both a potential biomarker and therapeutic target in many types of cancer. Fascin is most commonly associated with the formation of filopodia and invadapodia (parallel actin filament bundle structures) to drive migration and invasion. However, Fascin activity and regulation remain poorly understood. In order for Fascin to be an effective target for cancer therapeutics, a better understanding of the mechanisms regulating Fascin activities in the cell is necessary. Prostaglandins (PGs) are short-lived lipid signaling molecules that mediate a wide range of biological activities. PGs act through G protein-coupled receptors to initiate signaling cascades that affect downstream targets, including actin cytoskeletal remodeling. Importantly, the key enzymes in the synthesis of PGs, cyclooxygenase (COX) 1 and 2, are the targets of non-steroidal anti-inflammatory drugs like aspirin. Interestingly, like Fascin, PGs have been independently implicated in cancer development and metastasis and aspirin may reduce the risk of aggressive cancer. However, the exact mechanisms by which PGs mediate cancer development are unknown. The work presented in this thesis focused on novel PG-dependent regulation and activity of Fascin. The research presented here utilized Drosophila oogenesis as a model system to analyze PG-dependent Fascin activity. Drosophila oogenesis is an ideal model in which to study the activity and regulation of actin binding proteins like Fascin. Oogenesis consists of 14 morphologically defined stages, which are observable many times over within a single isolated pair of ovaries. A developing follicle consists of 16 germline cells – 15 nurse, or support cells, and a single oocyte. The nurse cells are of particular interest because they are the sites of dynamic actin remodeling during mid-late oogenesis. During stage 10B, an array of radially-aligned actin filament bundles form at the nurse cell membranes and extend inwards towards the nucleus. A network of cortical actin is also strengthened during this stage. These actin structures are essential for the completion of oogenesis, and ultimately female fertility. Importantly, PGs and Fascin are required for this actin remodeling; genetic loss of Fascin or the Drosophila COX-like enzyme Pxt (Peroxinectin-like) leads to disruption of cytoplasmic actin remodeling, and ultimately, female sterility. Using this model system, work presented here describes the discovery of Fascin as a downstream target of PGs to promote actin bundle formation, described in Chapter 2. Additionally, Fascin is required for strengthening of the cortical actin network downstream of PGs. This observation is one of the first to describe a role for Fascin in a branched actin network. Additionally, Fascin is regulated by a specific PG – PGF2α – during S10B to promote follicle development. Finally, Chapter 2 shows that PGs target specific actin binding proteins to promote cytoskeletal remodeling; Villin, another actin bundling protein, does not interact with PGs. Chapter 3 describes the novel observation that Fascin localizes to the nucleus and the nuclear periphery in Drosophila nurse cells. This finding is significant, as it is the first to describe Fascin in a context other than cytoplasmic. Fascin localization in and around the nucleus is specific and dynamic, and changes throughout late stage oogenesis, suggesting regulated functions at these sites. Fascin localization is regulated by PGs, and loss of Pxt leads to reduced nuclear Fascin localization and failure to localize to the nuclear periphery. Additionally, Fascin has novel potential functions in the nucleus and at the nuclear periphery. Loss of Fascin leads to disruption of nucleolar morphology in the nurse cell nuclei. Additionally, loss of PGs, which cause reduced nuclear Fascin levels, also causes abnormal nucleolar morphology. These data suggest that PGs regulate Fascin to control nucleolar organization. At the nuclear periphery, Fascin localization requires components of the protein complex that links the nucleoplasm to the cytoplasm, termed the LINC complex. Loss of an essential LINC complex protein, Koi, leads to a loss of nuclear periphery Fascin localization. These data suggest that Fascin may be a novel component of the LINC complex. Finally, Chapter 4 describes regulation of Fascin by phosphorylation at conserved serine residues. PGs affect Fascin phosphorylation, and loss of PGs leads to more heavily phosphorylated Fascin. Additionally, phosphorylation of Fascin alters localization to the nucleus and to the nuclear periphery. These data suggest that one mechanism by which PGs regulate Fascin is to control its phosphorylation status to affect subcellular distribution. In summary, the work presented in this thesis has demonstrated novel regulation and function of the actin bundling protein Fascin using Drosophila oogenesis as a model. Importantly, these functions and regulation of Fascin are likely conserved in mammals, and may have implications in human health and disease. Continued study of the activity and regulation of actin binding proteins like Fascin in Drosophila will likely have great effect on our understanding of many human diseases.

The search for a theory of quantum gravity has persisted through the last century. Although many beautiful theories such as string theory and loop quantum gravity have been proposed, experimental evidence to support or refute these theories has been difficult to obtain. Searching for Lorentz invariance violation (LIV) is one of a limited number of experimental tests which can be used to search for evidence of quantum gravity since new physics may only be observable at energies well beyond those present in the most energetic astrophysical objects, which are far greater than the energies accessible in a terrestrial laboratory. One method of searching for LIV is to look for energy-dependent time delays in the arrival of high-energy photons from distant astrophysical sources. We search for Lorentz invariance violation (LIV) using VERITAS, an imaging atmospheric Cherenkov telescope (IACT) located in southern Arizona. Significant TeV gamma ray flaring activity was detected from the blazar Markarian 421 on the night of February 17, 2010 (MJD 55244), which presented a good opportunity to search for delays in the energetic emission. We demonstrate the performance of two different dispersion estimation algorithms and apply these algorithms to our data to search for LIV. We find that while the emission from Markarian 421 contains significant variability, a necessary condition for an LIV detection, the presence of a constant background flux severely limits our sensitivity. We expect our findings to be useful for guiding future LIV studies, especially those using IACT data. In the latter part of this work we discuss the alignment of ground-based gamma-ray telescopes and present a digital autocollimator which will be used in the alignment system of a next-generation IACT. The configuration of our autocollimator enables measurement of the angle formed between the planar surface of a distant reflector and the line of sight over a range of ±0.126° with a precision better than 5 arcsec. We present a detailed description of the instrument and its data acquisition software that was used during laboratory testing.

This dissertation offers an ethnographic account of "the world's largest annual human migration": the family reunion ritual practiced by hundreds of millions of Chinese rural-to-urban migrant workers, who work in cities and travel back to the countryside during the lunar New Year (the Spring Festival) to reunite with family members. The formation and practice of this ritual is situated in the particular historical moment of China's modernization when rural migrants have gained the freedom to leave the countryside but are met with difficulties in settling in the city and becoming urban citizens. Although migrant workers have contributed directly to China's burgeoning economy, without an institutionalized system that provides them security and full social rights, they experience prolonged liminality between the city and the countryside. The Spring Festival reunion offers migrant workers a once-in-a-year chance to achieve family unity, to reconnect with scattered kith and kin, and to temporarily actualize a sense of normalcy and continuity in the rural community. Drawing on theories of cultural communication, ritual, and family communication, I conceptualize the reunion ritual as a form of "lifeworld re-embedment" on China's pathway to individualization--a social process that engages in cultural resources to cope with the risks of modernity, bridging the disjuncture between the individual and the community. Built on interviews with migrant workers and participant observation of family reunions in a village in Central China, this dissertation examines the ritual forms, meanings, and functions of the reunion. I first examine the ritualization of the Spring Festival reunion at a national level, focusing on the spectacular movement of passengers during the Spring Festival travel season. I argue that the Spring Festival homecoming has transformed from a transportation issue to a pilgrimage-like national ritual, projecting an image of the collective pursuit of family cohesion and community integration. As a response to the unequal access to urban citizenship, returning to one's countryside home has also become an alternative way for migrant workers to claim their identities and to find a sense of belonging. In communicating about the family reunion, migrant workers employ culturally distinctive languages of place attachment and collectively used discourses of displacement to construct the meaning of home, separation, and unification. In addition, I explore family rituals performed during the reunion that help migrant workers reconnect with left-behind family members, fulfill family obligations, and create family unity. This study provides a more nuanced understanding of the paradoxical process of individualization in China, in which disembedded individuals have to depend on culturally bound integration provided by the institutions from which the disembedment occurs. In this process, ritual communication not only articulates the tension between the individual and the communal, but also functions as a powerful compensatory solution to the risks of family dislocation. By analyzing the Spring Festival reunion from a micro-level with a focus on how ritualized communication constructs, maintains, repairs, and changes social reality, this study also adds to the body of literature on cultural communication and family communication.

Sankofa, a proverb from the Akan language of Ghana, is one attribute of Ghanaian culture that lives on in Black America. Sankofa, symbolically depicted as a bird reaching backwards to scoop an egg, translates into English as "reach back and get it." As an autobiographical artist, I continue to "reach back" into black history. I research the biographies and autobiographies of other Black Americans--the stories that chronicle the lives of those who came before me. I research urban cultures and contemporary expressions of identity and courage. Through the creation of installation and performance art, I have learned to incorporate this research into my own expressions my visions of hope, discontent, healing and beauty.

Founded by George Cavender in 1971, the Big Ten Band Directors Association is one of the oldest associations of its kind. With a membership consisting of each institution’s Director of Bands, Athletic Band Director, and all other band faculty, the stature of its members, both past and current, is clear. These band directors are leaders in the profession and have helped the field develop into what it has become today through their positions at their respective flagship institutions of the Big Ten Athletic Conference. The BTBDA meets each year at the Midwest International Band and Orchestra Clinic in Chicago, IL, and its investment in sponsoring quality sessions at the clinic is well documented. The association has sponsored twenty-one sessions since 1986, featuring prominent composers such as Michael Colgrass, Karel Husa, Warren Benson, Gunther Schuller, and Frank Ticheli. Additionally, the association has shown its dedication to the advancement of the band repertoire through commissioning seven new works from 1986-2014, four of which were born out of a commissioning contest that spanned from 1998-2005. This study is the first of its kind to document a band association formed of members bound by a specific athletic conference. Research of the Big Ten Band Directors Association shows numerous examples of how a band conference association can contribute to different facets of the field (e.g. commissioning, clinic session sponsorship, etc.). The profession’s knowledge of the BTBDA is important as it highlights how these associations are contributing to the landscape of the field.

Since the digital revolution, there has been a significant impact on how we live, work and play. For designers, it has transformed design practices and created new opportunities. However, this can bring about new problems and challenges. How can we communicate effectively in the digital age with different media and technologies advancing so rapidly? With the overload of information from tablets, smartphones, computers and television, we often start to overlook information. From research I will argue techniques that can help with us understand information in the digital realm and prove the importance of expression within visual communication.