In this thesis, the effectiveness of microparticle retention aid systems comprising of different cationic starches (tapioca and waxy maize), cationic flocculants (flocculant 1, linear with low charge density and flocculant 2, branched with medium charge density polyacrylamides) and anionic colloidal silica in improving retention and drainage of thermomechanical pulp (TMP) suspensions loaded with precipitated calcium carbonate (PCC) is studied. While starch is primarily added as dry strength agent in PCC-filled TMP suspensions, it also has a significant role in improving retention and drainage. Tapioca starch, which has both amylose and amylopectin, is found to be a better retention and drainage aid than waxy maize starch, which only contains amylopectin. In the absence of starch, both flocculants are ineffective in improving retention and drainage. With starch, both flocculant and silica are significant in enhancing retention and drainage further. Increasing the dosage of either flocculant or silica generally increases retention as well as drainage. Flocculant 2 is found to give slightly worse total and filler retention but better drainage compared to flocculant 1. The dosage of flocculant 2 is only half that of flocculant 1 though – for this reason overall flocculant 2 is deemed more effective in improving retention and drainage than flocculant 1. Split starch addition, where a portion of the starch is premixed with PCC and the rest added to the pulp, causes a slight decrease in both retention and drainage. Therefore, should the papermaker decide to use this approach, the starch should be split in a 25:75 ratio between PCC and the pulp to minimize the negative effects. Due to the cost benefits of using increased amounts of PCC, it is desirable to increase PCC content beyond the standard currently used (250 kg/t OD pulp for communications-grade paper). However, at very high dosages of PCC (500 kg/t OD pulp), the best combination identified in this work (tapioca starch, flocculant 2 and silica) is unable to maintain good retention and drainage. Increasing retention aid dosages may help retain more PCC, however this tactic can rapidly become uneconomical. A new approach is thus needed to achieve such highly-filled papers. / Applied Science, Faculty of / Chemical and Biological Engineering, Department of / Graduate

As part of CSIR Biosciences’ interest in aroma chemicals, the processing of crude sulphated turpentine (CST) into high value aroma products was investigated. The primary target product, linalool, was obtained from a mixture of α- and β-pinene in four steps. It can be transformed into a wide range of high value added aroma chemicals. Isomerisation of linalool in the presence of a transition metal catalyst furnishes geraniol and nerol. The scientific work described in this report was part of a bigger project aimed at developing innovative processes to manufacture aroma, flavour & fragrance chemicals through beneficiation of industrial waste streams and other raw materials, available locally from the Forestry, Paper & Pulp industries. The evaluation of a process for the preparation of precursor aroma, flavour & fragrance compounds, in particular geraniol and nerol, from locally available raw materials and industrial waste streams, was investigated. Preparation of geraniol and nerol from linalool (ex α-pinene stream) was investigated using acids or organometallic complexes as catalysts for the corresponding isomerisation reaction. The investigation was conducted in an effort to find a less costly process utilising milder conditions than via the conventional cleavage of β-pinene to myrcene under extreme pyrolysis conditions (>650°C). The transformation of linalool to geraniol/nerol using mineral acids was found to be dominated by secondary reactions such as dehydration and cyclisation, resulting in poor product selectivities and yields. On the other hand, organometallic complexes, in particular vanadium-based complexes (e.g. (OV(OBu)3) produced satisfactory results in the preliminary assessment (conversion of 79.8 percent and selectivity of 98.3 percent). A set of statistically designed experiments was carried out on the (VO(BuO)3 + [(Bu)4N+]OH¯) catalyst system where three variables were tested, i.e. substrate concentration, temperature, and catalyst loading. The selected model for conversion was significant with the “Probability > F” being < 0.0001. The most important contributing variable to the model for conversion was temperature i.e. 83.9 percent. Temperature was still the most important variable for the selectivity response at 65.0 percent contribution level. The response surface generated for the selectivity response was flat indicating a robust method within the parameter range selected.

Chapter 1: When Environmental Subsidies Backfire: The Case of Black Liquor and the Alternative Fuel Mixture Tax Credit: In 2005, the US government introduced the Alternative Fuel Mixture Tax Credit (AFMTC), which paid fifty cents/gallon for alternative fuel that was mixed and burned with traditional fuel. The American chemical pulp industry, which has traditionally burned ‘Black Liquor’, a residue of the pulping process, was able to make large claims on this subsidy in 2009 by mixing diesel fuel into a process where it was not required. This scenario exhibits two main downfalls of environmental subsidies: (i) the majority of the subsidy is paid to free-riders, and (ii) there are strong incentives towards overproduction and increased pollution. In this paper, the value of the AFMTC per tonne of chemical pulp is computed and used in a simulation using the Global Forest Products Model (Buongiorno, 2001) to calculate the effects of the AFMTC on the American and Canadian chemical pulp industries. The simulation suggests that the total amount paid to the American chemical pulp industry was US$7.63 billion, that American chemical pulp production rose by 2.5 million tonnes from the baseline, and Canadian production and exports to the US fell by 285,000 and 255,000 tonnes respectively, costing Canadian chemical pulp producers US$132 million in lost production. Using Canadian chemical pulp production and pollutant release data, production/release averages were developed. These averages suggest that the increase in American chemical pulp production led to significant increase in releases of greenhouse gases, hydrogen sulfide, sulfur oxides, nitrogen oxides and particulate matter. Chapter 2: The Alternative Fuel Mixture Tax Credit and the Pulp and Paper Green Transformation Program: A Policy Comparison: In 2009, chemical pulp mills in the US and Canada were able to take advantage of two subsidy programs that paid mills fifty cents for every gallon of ‘Black Liquor’ burned during the pulping process. Black Liquor is a residue product from the production of chemical pulp, and is traditionally used as a fuel in further pulp production. These subsidies were the Alternative Fuel Mixture Tax Credit (AFMTC) in the US and the Pulp and Paper Green Transformation Program (PPGTP) in Canada. Even though the AFMTC was a subsidy that applied to many industries, and the PPGTP was only available to chemical pulp mills, in the context of the chemical pulp industry only, the subsidies were almost identical: Both paid mills the same amount for undertaking the same activity, and mills were able to claim the subsidies for roughly the same amount of time. The key difference between the two programs was stipulations on how the money was to be spent. In the US, the AFMTC was a refundable tax credit, and simply another source of revenue for a recipient mill. In Canada, a recipient of PPGTP funds was required to spend the money on some form of capital investment that would increase energy efficiency or lower pollution emissions from the mill. In this paper, we develop a theoretical model with a representative chemical pulp mill in order to compare the effects that these two subsidies would have on the mill’s production of pulp (and in turn its production and use of black liquor), and its decision in whether to invest in a one-time capital improvement that would increase energy efficiency at the mill. The results from this model show that the PPGTP was a more effective policy than the AFMTC, in that it encouraged increased investment in energy efficiency, whereas the AFMTC did not. However, the PPGTP provides incentives to increase production, similar to the AFMTC, in two out of three possible outcomes. Even though the PPGTP is shown to be more effective than the AFMTC, it still exhibits several properties inherent in a second-best policy - because the subsidy is tied to production, it encouraged increased production, and may not have allocated funding in an efficient manner. Chapter 3: Naming, Shaming, and Abatement: Do Published ‘Top Emitter’ Lists Provide Incentive for Increasing Pollution Prevention Activities? Several works have measured the effects that publically disclosed pollutant release information has on the firms and facilities reporting the information. Most of these studies quantify the negative response that the information garners with the media, through some measure of media coverage, and measure the negative impact that this attention has on the firm; for example, by showing a negative effect on the respective company’s publically traded stock performance. Subsequent studies have then shown how these negative impacts from publicity cause firms to report lower emissions levels in following years. What is currently missing from this body of literature, however, is an examination of the step in between the firm receiving negative press and reporting lower emissions, namely, increased pollution abatement efforts on behalf of the publically labeled facility. In this paper, we attempt to gain a better understanding of this relationship by examining Canadian self-reported facility level data on pollution prevention activities from the National Pollutant Release Inventory. Two consistent samples of data are used to empirically test the effects that ‘Top Ten’ emitter lists, published by Environment Canada in the late 1990’s, had on the number of pollution prevention activities undertaken by facilities in the following year. The results from this work were inconclusive: Between these two samples, there was a noticeable decrease in the amount of pollution prevention activities reported in years after the cessation of the Top Ten publications. Under certain model specifications, a facility that was labeled as a Top Ten emitter is estimated to have 20% more pollution prevention activities undertaken compared to a facility that was not so labeled. It was also shown that a firm that reported new pollution abatement activities also had a significant reduction in releases. This finding, along with the decreasing trend in reported activities, raises a significant policy issue, as measures that clearly reduce reported emissions are being reported with less frequency in Canada.

No description available.

Cellulose is one of the most abundant biopolymers on earth and is an important commodity for industries such as the pulp and paper industry. Cellulose is deposited into the plant cell walls by a complex of membrane bound enzymes known as cellulose synthases. A number of cellulose synthase (CesA) genes, which encode for different cellulose synthase proteins, have been identified from plant species such as Eucalyptus, Populus and Arabidopsis. Mutant and expression profile analysis of the CesA genes indicated that a set of three CesA genes are associated with secondary cell wall formation, while a different set of CesA genes are associated with primary cell wall formation. The aim of this study was to investigate the transcriptional regulation of the different members of the CesA gene family in Eucalyptus. The promoter regions were comparatively analysed with the orthologous regions in Arabidopsis and Populus using bioinformatics tools to identify putative regulatory motifs that playa role in CesA genes regulation. Six Eucalyptus CesA gene promoters were isolated using genome walking. The Eucalyptus promoter regions and the orthologous promoter regions from Populus and Arabidopsis were analysed using TSSP (Transcriptional start site plant promoter prediction) and NNPP (Neural network promoter prediction) software packages. The software packages predicted the transcriptional start sites of the genes and the core regulatory elements such as the TATA-box and initiator elements. The in silico results were compared among species and it was found that the predicted transcriptional start sites and the core elements of the CesA gene promoters showed substantial structural conservation. The promoter regions were used in a comparative in silico analysis with the orthologous promoter regions from Arabidopsis and Populus to identify putative regulatory motifs. This is the first study in which the promoters of the CesA gene family are characterized in Arabidopsis, Populus and Eucalyptus. Three software packages (Weeder, POCO and MotifSampler) were used to analyse the promoter regions and identify over-represented motif sequences. A number of key stem-specific and xylem-specific motifs such as the AC-motif and G-box motif were identified as well as a number of novel motifs. Although all of the predicted motifs identified here will have to be functionally tested, the results of this study provide a good map for directed deletion studies and functional testing of the CesA promoters. / Dissertation (MSc (Genetics))--University of Pretoria, 2009. / Genetics / unrestricted

This master’s thesis is aimed at process of enzymatic hydrolysis of lignocellulosic material – waste paper as a source of raw material for production of liquid biofuels. In the theoretical part of this work are summarized previously used methods of hydrolysis and lignocellulosic materials used for the process of hydrolysis as a source of fermentable sugars for fermentation technology. The different types of waste paper are evaluated from the composition and usability with consideration to the papermaking process in order to select the appropriate type of waste paper for the enzymatic hydrolysis process. In the next part of this work are suggested technological premises and procedures for the preparation of raw materials and the subsequent enzymatic hydrolysis of these pre–treated materials. In the experimental part were optimized parameters of enzymatic hydrolysis using the Novozymes company enzyme package. Enzymatic degradation of cellulose to reducing sugars was observed using Somogyi – Nelson method. For the verification of hydrolysis conditions were used model materials with high cellulose content – pulp and filter paper. Conditions, which seems to be the best after testing on the model materials, were verified on specific waste paper materials – offset cardboard, recycled paper, matte MYsol paper and for comparison again on model materials – pulp and filter paper. The highest yields was achieved with the use of cardboard, which was further tested using various combinations of pretreatment to material for purpose of increase the yields of hydrolysis.

Orientador: Milton Carlos Kuga / Resumo: Os objetivos do presente estudo foram avaliar os efeitos dos solventes para remoção de resíduos de cimento endodôntico à base de resina epóxi (Sealer Plus) da dentina da câmara pulpar e a repercussão sobre a interface adesiva, com o sistema adesivo Universal (Single Bond Universal e Ambar Universal). Foram selecionados 90 incisivos bovinos com coroa dental similar, os quais foram seccionados, obtendo-se fragmentos da câmara pulpar, na proporção de 5x5x5mm para avaliação da microscopia eletrônica de varredura (MEV), e seus efeitos sobre a dentina da câmara pulpar, por meio de microscopia EDX e mensuração da microdureza dentinária, após o uso dos protocolos de limpeza contendo acetato de amila (AA), acetona pura (AC) ou etanol a 95% (ET) e combinações entre cada um destes protocolos (soluções experimentais AA-AC, AA-ET, AC-ET e AA-AC-ET), além dos controles positivo (PC) e negativo (NC). Além disso, quarenta fragmentos de dentina foram impregnados com cimento endodôntico e removidos com os protocolos de limpeza: etanol a 95% (ET), xilol (XI), acetato de amila (AA) ou ação mecânica (PC) e dez espécimes não receberam tratamento (NC) para avaliação também da persistência de resíduos e a repercussão sobre a interface adesiva. A avaliação da persistência de resíduos foi realizada em MEV (500x) e após, foram obtidos 100 espécimes, onde 80 foram impregnados e submetidos aos protocolos de limpeza e 20, não. Em cada protocolo, os espécimes foram divididos em 2 subgrupos (n=10), de acord... (Resumo completo, clicar acesso eletrônico abaixo) / Mestre

This study examines the wastewater treatment system on BillerudKorsnäs integrated pulpand paper mill at Karlsborg, with focus on the effluent of suspended solids. Due to lowerregulatory limits for the emissions of suspended solids BillerudKorsnäs is looking forways to improve the efficiency of the wastewater treatment. Therefore, this study seeksto create a statistical model for the emissions of suspended solids and to find which factorsin the production, wastewater treatment and surrounding environment that affects theemissions. The purpose of the study is to extend the current understanding of the treatmentsystem to create a base for future decisions. The methods used for the modelling areElastic net regression and Random forest classifier, which were selected for the variableselection properties they provide. Unfortunately, the results show that the fitted modelscan only explain a small part of the variation of the emissions of suspended solids.The lack of fit in the models indicate that the current data is not sufficient to explain thevariation in the emissions of suspended solids. During the study deficiencies in the datacollection has been detected and improvements to overcome these deficiencies are proposedin the hope of creating more reliable data for the company and to create a betterbasis for future studies. / Denna studie undersöker avloppsvattenreningen på BillerudKorsnäs integrerade massaoch pappersbruket i Karlsborg, med fokus på utsläppen av suspenderade ämnen. Pågrund ut av sänkta gränser för utsläppen av suspenderade ämnen undersöker BillerudKorsnäsmöjligheter för att öka effektiviteten i avloppsvattenreningen. Därför försöker dennastudie att skapa en statistisk modell för utsläppen av suspenderade ämnen och undersökavilka faktorer i produktion, avloppsvattenrening, och omgivande miljö som påverkar utsläppen.Syftet med studien är att förbättra förståelsen för avloppsvattenreningen och attskapa beslutsunderlag för framtida förbättringar. Metoderna som används i modellernaär Elastic net regression och Random forest classifier, vilka valdes för deras variabel selectionegenskaper. Resultaten för modellerna visar att modellerna endast kan förklara enliten del av variationen i utsläpp av suspenderade ämnen. Det dåliga resultatet indikeraratt nuvarande data inte är tillräckligt för att skapa den typ av modeller som används. Understudien så har brister i datainsamlingen upptäckts, och förbättringar för att överbryggadessa brister är föreslagna med ändamål att göra insamlad data mer pålitlig och skapa enbättre bas för framtida studier.

This thesis explores how to use the dry nanoporous structure of cellulosic fibres in new types of composite materials. A large effort was also given on how to correctly characterize the structure of fibres where the wet structure has been preserved also in the dry state. Delignified wood fibres have an open fibrillar structure in their water-swollen state. In the present work, this open fibrillar structure was preserved in the dry state by performing a liquid exchange procedure and the samples were thereafter carefully dried with Ar(g). The samples of never-dried TEMPO-oxidized dissolving pulp had a specific surface area of 130 m2/g in the dry state, as measured using the Brunauer, Emmet, and Teller (BET) Nitrogen gas adsorption method. This open structure was also revealed using field emission scanning electron microscopy (FE-SEM). The water-swollen and dry open structures were thoroughly characterized for various pulps. A new method for determining the pore size of water-swollen delignified cellulosic fibres is presented. By combining the results from solid state nuclear magnetic resonance NMR, measuring the specific surface area [m2/g] in the water-swollen state, with fibre saturation point (FSP), measuring the pore volume of fibres in water-swollen state [mass water/mass fibre], the average pore size can be determined without the need of assuming a certain pore geometry. The dry nanoporous structure was then used as a scaffold for in-situ polymerization, to demonstrate how the properties of the fibrils in the fibre wall can be exploited without the need to disintegrate the fibre wall. Both poly(methylmethacrylate) (PMMA) and poly(butylacrylate) (PBA) were successfully used as the polymeric matrix, and both nanocomposites (i.e., fibre/PMMA and fibre/PBA) had a fibre content of approximately 20 w%. The structure of the composites was characterized using SEM and Atomic Force Microscopy (AFM) operated in the phase imaging mode. The AFM results indicate that the cellulose aggregates and polymeric matrix were successfully mixed on a nanoscale, creating a nanocomposite of interpenetrating polymer molecules and cellulose fibrils, rather than a microcomposite, when using microscopic cellulose fibres. The water absorption capacity of the nanocomposites was reduced significantly, indicating that almost all nanopores in the fibre wall were successfully filled with matrix polymer. The mechanical properties were investigated, showing the importance of nanosized reinforcement compared to fibres of micrometer size. / <p>QC 20121011</p>

Magister Scientiae Dentium - MSc(Dent) / Background: The primary goal of pulp therapy in the deciduous dentition is to keep the teeth fully functional in order to prevent arch space loss. A pulpectomy is a pulp therapy procedure indicated when an irreversibly inflamed or necrotic radicular pulp is encountered. ZOE and iodoform pastes (i.e. Kri 1 paste and Vitapex) have been recommended as root filling materials after pulpectomy. High clinical success rates have been reported with Vitapex and the fact that it resorbs readily when extruded beyond the apex is an added advantage. However, it has also been reported to resorb from within the root canals and even cause pathological root resorption in cases where the vital pulp is inflamed (Nurko et al., 2000). Iodoform-based preparations are thought to be more appropriate because they fulfill nearly all the requirements of the ideal root filling materials in primary teeth. Despite this, there are limited studies comparing the antibacterial effect of iodoform-based preparations. Aim: The aim of this study was to evaluate the antibacterial efficacy of Vitapex (V), Kri 1 paste (K) and pure iodoform (I) against E. faecalis.