This project was formulated with the purpose of advancing knowledge of the invasion dynamics of rats within archipelagos in New Zealand. The concentration on islands reflected the conservation focus of this project - islands are the last refuges for many native New Zealand species that cannot survive in the wild on the mainland. This project can be divided into four areas: 1. Data collection: There was no intent for innovation here, but a deeper understanding of the environments in which rats are born, breed, migrate, and die was developed. 2. Development of tools for data exploration: • A user-friendly point-and-click graphical interface for the R program was designed to allow any user to easily explore simple genetic characteristics of the data. • A novel method for exploring the genetic similarity between individuals was developed and showcased with real data, proving successful in cases of both high and low genetic differentiation, and in detecting likely individual migrants. 3. Improvement of a method for estimating migration: • An attempt was made to improve the Markov chain Monte Carlo procedure underlying this method. • The migration model used by the method was significantly improved, so that it could cope with any level of migration. Previously, results from situations where migration rates were high were invalid. 4. Investigated topics of ecological interest: • Field measurements of rats were used to show that Norway rats tend to have larger masses than ship rats, southern rats are generally larger than northern rats, but the effect on mass of living on an island as opposed to the mainland depends on the latitude. It was also shown that relative tail length is a good species discriminator. • Multiple paternity was confirmed for both Norway and ship rats. This breeding characteristic might form part of the explanation for why rats are such successful invaders. During the project, case studies involving rats on Big South Cape Island, Great Barrier Island and in the Bay of Islands were used to highlight the methods developed, and provided some unexpected and fascinating results.

This project was formulated with the purpose of advancing knowledge of the invasion dynamics of rats within archipelagos in New Zealand. The concentration on islands reflected the conservation focus of this project - islands are the last refuges for many native New Zealand species that cannot survive in the wild on the mainland. This project can be divided into four areas: 1. Data collection: There was no intent for innovation here, but a deeper understanding of the environments in which rats are born, breed, migrate, and die was developed. 2. Development of tools for data exploration: • A user-friendly point-and-click graphical interface for the R program was designed to allow any user to easily explore simple genetic characteristics of the data. • A novel method for exploring the genetic similarity between individuals was developed and showcased with real data, proving successful in cases of both high and low genetic differentiation, and in detecting likely individual migrants. 3. Improvement of a method for estimating migration: • An attempt was made to improve the Markov chain Monte Carlo procedure underlying this method. • The migration model used by the method was significantly improved, so that it could cope with any level of migration. Previously, results from situations where migration rates were high were invalid. 4. Investigated topics of ecological interest: • Field measurements of rats were used to show that Norway rats tend to have larger masses than ship rats, southern rats are generally larger than northern rats, but the effect on mass of living on an island as opposed to the mainland depends on the latitude. It was also shown that relative tail length is a good species discriminator. • Multiple paternity was confirmed for both Norway and ship rats. This breeding characteristic might form part of the explanation for why rats are such successful invaders. During the project, case studies involving rats on Big South Cape Island, Great Barrier Island and in the Bay of Islands were used to highlight the methods developed, and provided some unexpected and fascinating results.

This project was formulated with the purpose of advancing knowledge of the invasion dynamics of rats within archipelagos in New Zealand. The concentration on islands reflected the conservation focus of this project - islands are the last refuges for many native New Zealand species that cannot survive in the wild on the mainland. This project can be divided into four areas: 1. Data collection: There was no intent for innovation here, but a deeper understanding of the environments in which rats are born, breed, migrate, and die was developed. 2. Development of tools for data exploration: • A user-friendly point-and-click graphical interface for the R program was designed to allow any user to easily explore simple genetic characteristics of the data. • A novel method for exploring the genetic similarity between individuals was developed and showcased with real data, proving successful in cases of both high and low genetic differentiation, and in detecting likely individual migrants. 3. Improvement of a method for estimating migration: • An attempt was made to improve the Markov chain Monte Carlo procedure underlying this method. • The migration model used by the method was significantly improved, so that it could cope with any level of migration. Previously, results from situations where migration rates were high were invalid. 4. Investigated topics of ecological interest: • Field measurements of rats were used to show that Norway rats tend to have larger masses than ship rats, southern rats are generally larger than northern rats, but the effect on mass of living on an island as opposed to the mainland depends on the latitude. It was also shown that relative tail length is a good species discriminator. • Multiple paternity was confirmed for both Norway and ship rats. This breeding characteristic might form part of the explanation for why rats are such successful invaders. During the project, case studies involving rats on Big South Cape Island, Great Barrier Island and in the Bay of Islands were used to highlight the methods developed, and provided some unexpected and fascinating results.

This project was formulated with the purpose of advancing knowledge of the invasion dynamics of rats within archipelagos in New Zealand. The concentration on islands reflected the conservation focus of this project - islands are the last refuges for many native New Zealand species that cannot survive in the wild on the mainland. This project can be divided into four areas: 1. Data collection: There was no intent for innovation here, but a deeper understanding of the environments in which rats are born, breed, migrate, and die was developed. 2. Development of tools for data exploration: • A user-friendly point-and-click graphical interface for the R program was designed to allow any user to easily explore simple genetic characteristics of the data. • A novel method for exploring the genetic similarity between individuals was developed and showcased with real data, proving successful in cases of both high and low genetic differentiation, and in detecting likely individual migrants. 3. Improvement of a method for estimating migration: • An attempt was made to improve the Markov chain Monte Carlo procedure underlying this method. • The migration model used by the method was significantly improved, so that it could cope with any level of migration. Previously, results from situations where migration rates were high were invalid. 4. Investigated topics of ecological interest: • Field measurements of rats were used to show that Norway rats tend to have larger masses than ship rats, southern rats are generally larger than northern rats, but the effect on mass of living on an island as opposed to the mainland depends on the latitude. It was also shown that relative tail length is a good species discriminator. • Multiple paternity was confirmed for both Norway and ship rats. This breeding characteristic might form part of the explanation for why rats are such successful invaders. During the project, case studies involving rats on Big South Cape Island, Great Barrier Island and in the Bay of Islands were used to highlight the methods developed, and provided some unexpected and fascinating results.

No description available.

La lutte contre les rongeurs est souvent un enjeu majeur dans les agroécosystèmes. Nous avons conduit une étude comparative dans des plantations de palmiers à huile dans les provinces de Riau et de Bangka en Indonésie. Dans les deux zones, des chouettes ont été introduites pour la lutte contre les rats ; toutefois, à Riau les rats sont maintenus à des niveaux de population acceptables sans recours au raticide, tandis qu’à Bangka les dégâts des rats sont très importants et l’usage de raticide intensif. Nous avons comparé ce deux systèmes en terme d’abondance et/ou de régime alimentaire de deux prédateurs, chouettes et petits carnivores. D’après nos résultats, les petits carnivores sont beaucoup plus abondants dans les plantations à Riau qu’à Bangka, et le chat léopard (Prionailurus bengalensis) est absent à Bangka tandis que cette espèce domine à Riau. Nos résultats suggèrent également que la prédation sur les rats par les chouettes et les petits carnivores serait moindre à Bangka qu’à Riau. D’une manière générale, cette étude confirme l’hypothèse selon laquelle les petits carnivores, notamment les chats léopard, joueraient un rôle important dans la lutte contre les rats en palmeraies. Par ailleurs, nous avons analysé la distribution spatiale des petits carnivores en plantation. Nos résultats suggèrent que, bien que l’habitat palmeraie soit largement utilisé la nuit par certains petits carnivores comme le chat leopard, qui y trouve une abondante ressource alimentaire, la plupart des espèces sont dépendantes de la forêt. Les gestionnaires des palmeraies devraient adapter leurs pratiques, afin de favoriser les petits carnivores dans une perspective de lutte contre les rats. / Rodent pest control is often a major issue in agroecosystems. We conducted a 3-year comparative study (2010-2012) in oil palm plantations in Riau and Bangka provinces, in Indonesia: in both areas barn owls have been introduced for rat control, and were at least as abundant in Bangka plantations than in Riau, but in Riau rat populations have been maintained at an acceptable level without the use of rodenticide, whereas in Bangka intensive rodenticide applications did not prevent high levels of rat damage. We compared these two contrasting systems in terms of predator community (barn owls and small carnivores) abundance and/or diet. We found that small carnivores were much more abundant in Riau plantations than in Bangka, and that the leopard cat (Prionailurus bengalensis) was the dominant species in Riau while absent from Bangka. Our results on diet suggested that rat prey intake from barn owls and from the small carnivore community would be less in Bangka plantations than in Riau. Broadly, our results suggest that small carnivores, notably the leopard cat, play an important role in rodent control. In addition, we investigated spatial distribution of small carnivores within the oil palm habitat. Our results support the hypothesis that, although the oil palm may be habitable for some small carnivore species such as the leopard cat, where they supposedly forage at night, most species still need forest for their survival in oil palm landscapes. Oil palm plantations managers should adapt agricultural practices and land-use to enhance small carnivores, with the view to improve rodent control.

\ / Doctorat en Sciences / info:eu-repo/semantics/nonPublished

L'incessant augment del nombre de seqüències genòmiques, juntament amb l'increment del nombre de tècniques experimentals de les que es disposa, permetrà obtenir el catàleg complet de les funcions cel.lulars de diferents organismes, incloent-hi la nostra espècie. Aquest catàleg definirà els fonaments sobre els que es podrà entendre millor com els organismes funcionen a nivell molecular. Al mateix temps es tindran més pistes sobre els canvis que estan associats amb les malalties. Per tant, la seqüència en brut, tal i com s'obté dels projectes de seqüenciació de genomes, no té cap valor sense les anàlisis i la subsegüent anotació de les característiques que defineixen aquestes funcions. Aquesta tesi presenta la nostra contribució en tres aspectes relacionats de l'anotació dels gens en genomes eucariotes. Primer, la comparació a nivell de seqüència entre els genomes humà i de ratolí es va dur a terme mitjançant un protocol semi-automàtic. El programa de predicció de gens SGP2 es va desenvolupar a partir d'elements d'aquest protocol. El concepte al darrera de l'SGP2 és que les regions de similaritat obtingudes amb el programa TBLASTX, es fan servir per augmentar la puntuació dels exons predits pel programa geneid, amb el que s obtenen conjunts d'anotacions més acurats d'estructures gèniques. SGP2 té una especificitat que és prou gran com per que es puguin validar experimentalment via RT-PCR. La validació de llocs d'splicing emprant la tècnica de la RT-PCR és un bon exemple de com la combinació d'aproximacions computacionals i experimentals produeix millors resultats que per separat. S'ha dut a terme l'anàlisi descriptiva a nivell de seqüència dels llocs d'splicing obtinguts sobre un conjunt fiable de gens ortòlegs per humà, ratolí, rata i pollastre. S'han explorat les diferències a nivell de nucleòtid entre llocs U2 i U12, pel conjunt d'introns ortòlegs que se'n deriva d'aquests gens. S'ha trobat que els senyals d'splicing ortòlegs entre humà i rossegadors, així com entre rossegadors, estan més conservats que els llocs no relacionats. Aquesta conservació addicional pot ser explicada però a nivell de conservació basal dels introns. D'altra banda, s'ha detectat més conservació de l'esperada entre llocs d'splicing ortòlegs entre mamífers i pollastre. Els resultats obtinguts també indiquen que les classes intròniques U2 i U12 han evolucionat independentment des de l'ancestre comú dels mamífers i les aus. Tampoc s'ha trobat cap cas convincent d'interconversió entre aquestes dues classes en el conjunt d'introns ortòlegs generat, ni cap cas de substitució entre els subtipus AT-AC i GT-AG d'introns U12. Al contrari, el pas de GT-AG a GC-AG, i viceversa, en introns U2 no sembla ser inusual. Finalment, s'han implementat una sèrie d'eines de visualització per integrar anotacions obtingudes pels programes de predicció de gens i per les anàlisis comparatives sobre genomes. Una d'aquestes eines, el gff2ps, s'ha emprat en la cartografia dels genomes humà, de la mosca del vinagre i del mosquit de la malària, entre d'altres. El programa gff2aplot i els filtres associats, han facilitat la tasca d'integrar anotacions de seqüència amb els resultats d'eines per la cerca d'homologia, com ara el BLAST. S'ha adaptat també el concepte de pictograma a l'anàlisi comparativa de llocs d splicing ortòlegs, amb el desenvolupament del programa compi. / El aumento incesante del número de secuencias genómicas, junto con el incremento del número de técnicas experimentales de las que se dispone, permitirá la obtención del catálogo completo de las funciones celulares de los diferentes organismos, incluida nuestra especie. Este catálogo definirá las bases sobre las que se pueda entender mejor el funcionamiento de los organismos a nivel molecular. Al mismo tiempo, se obtendrán más pistas sobre los cambios asociados a enfermedades. Por tanto, la secuencia en bruto, tal y como se obtiene en los proyectos de secuenciación masiva, no tiene ningún valor sin los análisis y la posterior anotación de las características que definen estas funciones. Esta tesis presenta nuestra contribución a tres aspectos relacionados de la anotación de los genes en genomas eucariotas. Primero, la comparación a nivel de secuencia entre el genoma humano y el de ratón se llevó a cabo mediante un protocolo semi-automático. El programa de predicción de genes SGP2 se desarrolló a partir de elementos de dicho protocolo. El concepto sobre el que se fundamenta el SGP2 es que las regiones de similaridad obtenidas con el programa TBLASTX, se utilizan para aumentar la puntuación de los exones predichos por el programa geneid, con lo que se obtienen conjuntos más precisos de anotaciones de estructuras génicas. SGP2 tiene una especificidad suficiente como para validar esas anotaciones experimentalmente vía RT-PCR. La validación de los sitios de splicing mediante el uso de la técnica de la RT-PCR es un buen ejemplo de cómo la combinación de aproximaciones computacionales y experimentales produce mejores resultados que por separado. Se ha llevado a cabo el análisis descriptivo a nivel de secuencia de los sitios de splicing obtenidos sobre un conjunto fiable de genes ortólogos para humano, ratón, rata y pollo. Se han explorado las diferencias a nivel de nucleótido entre sitios U2 y U12 para el conjunto de intrones ortólogos derivado de esos genes. Se ha visto que las señales de splicing ortólogas entre humanos y roedores, así como entre roedores, están más conservadas que las no ortólogas. Esta conservación puede ser explicada en parte a nivel de conservación basal de los intrones. Por otro lado, se ha detectado mayor conservación de la esperada entre sitios de splicing ortólogos entre mamíferos y pollo. Los resultados obtenidos indican también que las clases intrónicas U2 y U12 han evolucionado independientemente desde el ancestro común de mamíferos y aves. Tampoco se ha hallado ningún caso convincente de interconversión entre estas dos clases en el conjunto de intrones ortólogos generado, ni ningún caso de substitución entre los subtipos AT-AC y GT-AG en intrones U12. Por el contrario, el paso de GT-AG a GC-AG, y viceversa, en intrones U2 no parece ser inusual. Finalmente, se han implementado una serie de herramientas de visualización para integrar anotaciones obtenidas por los programas de predicción de genes y por los análisis comparativos sobre genomas. Una de estas herramientas, gff2ps, se ha utilizado para cartografiar los genomas humano, de la mosca del vinagre y del mosquito de la malaria. El programa gff2aplot y los filtros asociados, han facilitado la tarea de integrar anotaciones a nivel de secuencia con los resultados obtenidos por herramientas de búsqueda de homología, como BLAST. Se ha adaptado también el concepto de pictograma al análisis comparativo de los sitios de splicing ortólogos, con el desarrollo del programa compi. / The constantly increasing amount of available genome sequences, along with an increasing number of experimental techniques, will help to produce the complete catalog of cellular functions for different organisms, including humans. Such a catalog will define the base from which we will better understand how organisms work at the molecular level. At the same time it will shed light on which changes are associated with disease. Therefore, the raw sequence from genome sequencing projects is worthless without the complete analysis and further annotation of the genomic features that define those functions. This dissertation presents our contribution to three related aspects of gene annotation on eukaryotic genomes. First, a comparison at sequence level of human and mouse genomes was performed by developing a semi-automatic analysis pipeline. The SGP2 gene-finding tool was developed from procedures used in this pipeline. The concept behind SGP2 is that similarity regions obtained by TBLASTX are used to increase the score of exons predicted by geneid, in order to produce a more accurate set of gene structures. SGP2 provides a specificity that is high enough for its predictions to be experimentally verified by RT-PCR. The RT-PCR validation of predicted splice junctions also serves as example of how combined computational and experimental approaches will yield the best results. Then, we performed a descriptive analysis at sequence level of the splice site signals from a reliable set of orthologous genes for human, mouse, rat and chicken. We have explored the differences at nucleotide sequence level between U2 and U12 for the set of orthologous introns derived from those genes. We found that orthologous splice signals between human and rodents and within rodents are more conserved than unrelated splice sites. However, additional conservation can be explained mostly by background intron conservation. Additional conservation over background is detectable in orthologous mammalian and chicken splice sites. Our results also indicate that the U2 and U12 intron classes have evolved independently since the split of mammals and birds. We found neither convincing case of interconversion between these two classes in our sets of orthologous introns, nor any single case of switching between AT-AC and GT-AG subtypes within U12 introns. In contrast, switching between GT-AG and GC-AG U2 subtypes does not appear to be unusual. Finally, we implemented visualization tools to integrate annotation features for gene- finding and comparative analyses. One of those tools, gff2ps, was used to draw the whole genome maps for human, fruitfly and mosquito. gff2aplot and the accompanying parsers facilitate the task of integrating sequence annotations with the output of homologybased tools, like BLAST.We have also adapted the concept of pictograms to the comparative analysis of orthologous splice sites, by developing compi.