The diploma thesis deals with the development of a new masonry system, for the production of which recycled brick could be used as a secondary raw material. In the field of literary research, the work deals with the methods of construction of vertical structures, the possibilities of lightening concrete, the life cycle of building materials and the use of secondary raw materials in construction. In the practical part, a masonry system was designed based on market research in the field of internal non-load-bearing masonry structures and requirements for these structures. In order to achieve these properties, concrete was chosen as the material for its production, where recycled brick was used as an alternative aggregate. As it is supposed to be a system for precise masonry, the material was compacted by vibrocompression. At the beginning, recipes were designed to verify the basic properties of concrete: density of hardened concrete, compressive strength and tensile bending strength, then other properties such as thermal conductivity and sound absorption coefficient were tested on improved recipes. Lightening and dimensional modules have been modified to achieve optimal properties. This was followed by a proposal for the method of masonry and surface treatment of a wall. Finally, the economic and environmental aspects of the masonry system were evaluated.

Using flake dimensions and attributes commonly agreed are associated with site use, occupation age, and occupation duration, it was argued that relative estimations of site function and occupation age could be determined using debitage. This is particularly beneficial for assemblages that have little to no diagnostics that could provide a general cultural period for one or more occupations at a site. The results of this study suggest that, although certain attributes are generally associated with lithic production stage, relative age, and duration indicators, they were not all applicable within this study. The methods employed were relatively successful; however, reducing the number of classes, removing of a dimension, and more sites that meet the definition of lithic scatter is needed. Furthermore, testing occupation duration using the number of breaks on a flake is not possible unless it is proven a single occupation site.

Denna studie undersöker och diskuterar möjligheterna som finns för att tala om ett icke-verbalt visuellt identitetsspråk för kammaterial från perioden ca 700–900 e.v.t. Studien utgår från och analyserar arkeologisk forskning som utförts i olika miljöer. Syftet är att se till potentialen som finns för vidare forskning gällande kammar, råmaterial och ornamentiks delaktighet i identitetsprocesser. Genom att studera vardagliga föremål som kammar med nya metoder och teknologi ökar möjligheterna att studera subtila sociala och kulturella fenomen. Undersökningen ämnar därför att även belysa hur nya frågor kan tillkomma och ställas till äldre väl undersökt material. / The aim of this study is to discuss and explore the possibility of a non-verbal language based on visuality in comb material dating to c. 700-900 C.E. The study analyses different archaeological research done in different locales within and outside of Scandinavia. The aim is to understand the potential of further research into identity studies based on previously made associations and interpretations regarding combs, raw material and decoration. Through new technologies, the study of everyday objects of the past can be given new context and meaning. This study aims to bring forth the potential in old well documented material.

This diploma thesis focuses on biogas stations used in agriculture. The thesis is divided into two parts - the literary research and the practical part. The first part of the thesis introduces the overall description of the biogas, its production, definition, and use. Moreover, the input and output raw materials of a biogas station are described. Subsequently, the operation and the chronological procedure used during the biogas production and resulting electricity in biogas station are introduced. The practical part of the thesis focuses on the actual situation in Europe and the development of both, the biogas stations' construction and the production of electricity from biomass. This part also analyses preferred raw materials of selected countries of the EU. Electrical capacity (IEC) and a particular state subsidy policy are also mentioned in the thesis. The research also includes particular examples of a good attitude and use of biogas stations in practice. In detail, the thesis deals with how these topics are dealt with in selected countries. In the Czech Republic, Slovakia, Germany, and Poland. In conclusion, according to the ascertain data, the overall summary and prognosis for the future are introduced.

La découverte du site Nepress (BiEr-21) en 2004 et les saisons de fouilles subséquentes ont permis de découvrir de nombreux vestiges archéologiques. Ce mémoire a donc pour objectif de déterminer l’identité culturelle des occupants qui ont fréquenté le site, en prenant en considération les activités rituelles et la stratégie d’approvisionnement en matière lithique. Pour y parvenir, une analyse morpho-métrique de l’assemblage lithique a été effectuée. La distribution intra-site des artéfacts a également été prise en considération lors de l’analyse. Une séquence chronologique du Nord-Est américain remontant au Sylvicole inférieur est présentée dans ce mémoire. Une période d’occupation semble dominer sur le site Nepress, soit le Sylvicole inférieur. Cette manifestation est caractérisée par la présence d’artéfacts diagnostiques de la culture Meadowood. Ces objets sont un grattoir triangulaire bifacial Meadowood, ainsi qu’une imitation de pointe de type box-base. / The discovery of the Nepress site (BiEr-21) in 2004 and the subsequent excavations have revealed many archaeological remains. This thesis has seeks to determine the cultural identity of the site’s occupants, taking into account their ritual activities and their lithic procurement strategy. To achieve this, a morpho-metric analysis of the lithic assemblage was undertaken. The intra-site artifact distribution was also taken into account. A chronological sequence for Northeastern North America going back to the Early Woodland is presented. The Early Woodland appears to dominate the occupation of the Nepress site. This is characterised by the presence of diagnostic artifacts of the Meadowood culture. These objects are a triangular bifacial Meadowood scraper, as well as an imitation of a projectile point.

A utilização de sondas fluorescentes para marcação ou detecção de biomoléculas de interesse em processos biológicos distintos, vem recebendo grande atenção em pesquisas biomédicas, de análises clínicas e biologia celular. Em geral, estas sondas fluorescentes são constituídas por moléculas orgânicas pequenas, as quais apresentam características fluorescentes e capacidade de conjugar-se com estas biomoléculas. Desta forma, esta dissertação descreve inicialmente a síntese de compostos 2-aril ou 2,5- diarilfuranos e tiofenos (3, 5, 7 e 9a-i), a partir de espécies orgânicas de telúrio (1, 4, 6 e 8) e sais de ariltrifluoroboratos de potássio (2a-i), via reação de acoplamento cruzado tipo Suzuki-Miyaura, sob catálise de paládio [ver a figura no arquivo original]. Em seguida, descreve a síntese de um derivado 2,5-diarilfurano assimétrico, o 2-(3-aminofenil)-5-(4-metoxifenil)furano 11, o qual apresenta propriedades químicas e luminescentes adequadas para atuar como uma nova e promissora sonda fluorescente em processos de marcação ou detecção biológica. A síntese desta nova sonda, também chamada de 3-AFA (3-AnililFuranoAnisol), foi realizada a partir da reação de acoplamento cruzado tipo Suzuki-Miyaura, sob catálise de paládio, entre o ácido (3-aminofenil) borônico 5e e o 2- (butiltelanil)-5-(4-metoxifenil)furano 10 em 65 % de rendimento. Esta nova sonda 3-AFA 11 foi conjugada com uma série de L-aminoácidos 12a-l, a partir de uma reação de acoplamento peptídico para formação dos respectivos produtos 13a-l em rendimentos satisfatórios. Estes produtos conjugados 13a-l, apresentam potencial para marcação fluorescente de enzimas proteolíticas de interesse [ver a figura no arquivo original]. Adicionalmente, foram caracterizadas propriedades fotofísicas importantes desta nova sonda 3-AFA 11, entre as quais seus espectros eletrônicos de absorção e emissão de fluorescência, que obtiveram valores de λex a 320 nm e λem a 400 nm, respectivamente. Por fim, foi testado o potencial de acúmulo intracelular da sonda 3-AFA 11 em sistemas celulares tais como: eritrócitos infectados com Plasmodium chabaudi e amastigotas de Leishmania L. amazonensis, sendo possível observar em todos os casos a marcação fluorescente dos respectivos parasitas. / The use of fluorescent probes for labeling or detection of biomolecules of interest in different biological processes, has received much attention in biomedical, clinical testing and cell biology research. In general, these fluorescent probes are composed of small organic molecules, which have fluorescent features and ability to combine with these biomolecules. Thus, this work initially describes the synthesis of 2-aryl or 2,5-diarylfurans and thiophenes (3, 5, 7 and 9a-i) from organic species of tellurium (1, 4, 6 and 8) and potassium organotrifluoroborate salts (2a-i) by palladium catalyzed Suzuki-Miyaura crosscoupling reaction [see the figure in the original file]. After describe the synthesis of a unsymmetrical 2,5-diarylfuran derivative, the 2-(3-aminophenyl)-5-(4-methoxyphenyl)furan 11, which presents chemical and luminescent properties appropriate to act as a promising new fluorescent probes in processes of marking or biological detection. The synthesis of this new probe, also called 3-AFA (3-AnilylFuranAnisole), was performed from palladium catalyzed Suzuki-Miyaura cross-coupling reaction, between (3- aminophenyl) boronic acid 5e and 2-(butyltellanyl)-5-(4-metoxyphenyl)furan 10 in 65 % yields. This new probe 3-AFA 11 was combined with a series of Lamino acids 12a-l from a peptidic coupling reaction to obtain their products 13a-l in satisfactory yield. These combined products 13a-l show a potential for fluorescent marking of proteolitic enzymes [see the figure in the original file]. Additionally, important photo physical properties of this new probe 3-AFA 11 were characterized. These include their electronic spectra of absorption and fluorescence emission, who obtained values of λex to 320 nm and λem to 400 nm, respectively. Finally, we tested the potential of probe 3-AFA 11 for intracellular accumulation in cellular systems such as: erythrocytes infected with Plasmodium chabaudi and amastigotes of Leishmania L. amazonensis, in all cases the fluorescent uptake cellular of their parasites can be observed.

Le recyclage des déchets plastiques issus des déchets d'équipements électriques et électroniques (D3E) est devenu un enjeu majeur de nos sociétés afin de limiter leur impact environnemental. Le recyclage mécanique apparaît aujourd’hui comme une solution mature industriellement et adaptée pour produire de matières premières de recyclage (MPR). L’objectif de cette thèse est la compatibilisation de mélange de plastiques issus de la filière de tri des D3E en vue de leur valorisation par recyclage mécanique. La caractérisation des mélanges D3E par des analyses FT-IR, fluorescence-X, ATG et DSC a montré qu’il s’agissait de mélanges complexes contenant majoritairement des polymères styréniques et des polyoléfines et de faible pourcentage de PVC, PMMA, PC… Ces mélanges, mis en œuvre, présentent des propriétés mécaniques très faibles par rapport aux polymères techniques et de commodité. Ces résultats ont démontré la nécessité d’une compatibilisation de ces D3E en vue de leur transformation en MPR. Au vu de la complexité des mélanges, la compatibilisation in situ à l’aide de réactions radicalaires est apparue particulièrement prometteuse. Une première approche a consisté à générer les radicaux par irradiation puis, en deuxième approche, par ajout d’amorceurs chimiques. Ces deux approches n’ont pas produit d’améliorations significatives des propriétés. Suite à ces résultats, des essais de compatibilisation par ajout de copolymères ont été entrepris, conduisant à une amélioration importante des propriétés mécaniques. La réalisation de prototypes techniques, à partir de MPR compatibilisés, a été validée et permet de démontrer leur potentiel technique. / The recycling of the plastics from waste electrical and electronic equipment (WEEE) has attracted great attention recently for environmental reasons. Mechanical recycling has emerged as the most economical, as well as the most energetic and ecologically efficient option. The aim of this work is the mechanical recycling of WEEE mixed plastic waste (MPW) streams. First, MPW composition has been evaluated by FT-IR and X-ray fluorescence spectroscopies, and ATG and DSC analyses. MPW are complex blend composed of thermoplastics, mainly styrenic polymers (ABS, HIPS, PS) and polyolefins. The remaining were other thermoplastics as PVC, PE, PMMA, PC…. After processing, these mixtures show very low mechanical properties compared to commodity and engineering thermoplastics. These results demonstrated that compatibilization is necessary to transform these D3E into secondary raw materials (SRM). In situ compatibilization using radical reactions appeared to be an attractive solution. Two pathways have been studied to generate the radicals, firstly, by electron beam radiation and, secondly, by adding chemical initiators. These two approaches didn’t show any substantial improvement in mechanical properties. Following these results, compatibilization by addition of reactive and non-reactive commercial copolymers were undertaken, leading to a significant improvement in mechanical properties. The production of technical prototypes and 3D printing wires from compatibilized SRM has been validated and allows considering an industrial development.

O cloridrato de tramadol, analgésico sintético de ação central, possui dois centros quirais: o isômero cis é ativo e o isômero trans é uma impureza de processo. Ambos os enantiômeros do cloridrato de cis-tramadol contribuem para o efeito analgésico, mas através de mecanismos diferentes, complementares e interativos farmacologicamente. Os dois isômeros do cis-tramadol apresentam efeitos terapêuticos, e a presença de impurezas, incluindo os isômeros trans - decorrentes do processo de síntese ou devido à decomposição - podem comprometer a qualidade do produto comercializado. Assim, este trabalho teve como objetivo desenvolver e validar metodologia estereosseletiva para análise do cloridrato de cis-tramadol e das possíveis impurezas quirais ou não na matéria-prima e formulações farmacêuticas. Para a separação e quantificação dos enantiômeros do cloridrato de cis-tramadol e das impurezas trans-tramadol, 1,2-olefina e 1,6-olefina, foi utilizada a coluna Chiralcel® OD-H, fase móvel constituída por hexano (60% e 100% de n-hexano, 1:1, v/v):isopropanol:dietilamina:ácido trifluoracético (99,5:0,5:0,3:0,1, v/v/v/v), na vazão de 0,7 mL min-1 e detecção em 274 nm. A coluna Chiralpak® AD fase móvel constituída por hexano (60% de n-hexano):etanol absoluto:dietilamina (95:5:0,1, v/v/v), na vazão de 1,0 mL min-1 e o comprimento de onda para detecção dos compostos foi de 228 nm foi utilizada para a separação e quantificação das impurezas O-desmetiltramadol, N-desmetiltramadol e tramadol N-óxido. Os métodos desenvolvidos foram devidamente validados através dos parâmetros seletividade, linearidade, precisão, exatidão, intervalo, limite de detecção e limite de quantificação. Os resultados obtidos na validação mostraram que os métodos são adequados para a determinação do cis-tramadol e de suas impurezas na matéria prima e na formulação farmacêutica. / Tramadol hydrochloride is a centrally acting analgesic with two chiral centers; the cis isomer is the active drug and the trans isomer is a process impurity. Both enantiomers of cis-tramadol hydrochloride contribute to the analgesic effect through different, but complementary and interactive pharmacological mechanisms. Although both isomers of cis-tramadol hydrochloride show therapeutic effects, the presence of impurities, originated from the synthesis process or due to degradation, can compromise the quality of the marketed product. The aim of this present work was the development and validation of a stereosselective methodology for the analysis of the drug cis-tramadol hydrochloride and the possible chiral or non-chiral impurities in raw materials and pharmaceutical formulations. The separation and quantitation of cis-tramadol enantiomers and the impurities trans-tramadol, 1,2-olefin and 1,6-olefin were carried out using a Chiralcel® OD-H column, mobile phase of hexane (60% and 100% of n-hexane, 1:1, v/v):2-propanol:diethylamine:trifluoroacetic acid (99,5:0,5:0,3:0,1, v/v/v/v) at a flow rate of 0,7 mL min-1 and detection at 274 nm. For the separation and quantitation of the impurities O-desmethyltramadol, N-desmethyltramadol and tramadol N-oxide, a Chiralpak® AD column was used with a mobile phase of hexane (60% of n-hexane):ethanol absolute: diethylamine (95:5:0,1, v/v/v) at a flow rate of 1,0 mL min-1 and detection at 228 nm. The methods were validated using the parameters selectivity, linearity, precision, accuracy, range, detection limit and quantitation limit. The results obtained show that the methods are suitable for the analysis of cis-tramadol and its impurities in raw material and pharmaceutical formulation.

Im Forschungsprojekt wurde eine Bauweise für ein modular aufgebautes und flexibel einsetzbares Gestellsystem entwickelt, welches durch integrativen Leichtbau den vorteilhaften Einsatz von Holzfurnierlagenverbund-werkstoffen (WVC) für Verarbeitungs- und Fördermaschinen ermöglicht. Die ingenieurtechnisch relevanten Eigen-schaften des Holzbasiswerkstoffs (u.a. strukturelle Dämpfungseigenschaft) wurden ermittelt und darauf aufbauend ein Profil als Strukturelement des Gestellsystems entwickelt. Hier lag besonderes Augenmerk auf der Gestaltung des Profilquerschnitts. Es wurden verschiedene Querschnittsgeometrien vergleichend untersucht, wobei sich ein ge-schlossenes Kastenprofil als günstig erwies. Ausgehend vom entwickelten Profil wurde die für ein modulares Sys-tem notwendige Verbindungstechnik konzipiert. Folgend wurde schrittweise die modulare Bauweise in Strukturein-heiten umgesetzt sowie parallel Untersuchungen zu Steifigkeit und Festigkeit der Profile fortgeführt. Während der Erprobung von Struktureinheiten unter praxisnahen Bedingungen wurden gewisse konstruktive Verbesserungspo-tentiale deutlich. Diese Änderungen sowie die gewonnenen Erkenntnisse aus der Material- und Strukturprüfung kamen im Prototyp zur Umsetzung. Schallpegelprofile verschiedener fördertechnischer Anlagen und des entwickel-ten Prototypen wurden abschließend aufgenommen und verglichen. / Aim of the present study was to develop a modular designed and widely employable rack system. Positive properties of wood based materials (WVC) in lightweight structures were identified and integrated for the application in fabri-cation and conveyer technologies. For this purpose relevant properties of wood materials had been investigated (e.g. damping properties). The results of these analyses were the basis for the development of a beam profile, the basic structural design element for the future rack system. The most effort was put into finding the optimal beam cross section. Several different cross sections had been compared, a square sectional beam profile showed the best per-formance. Based on the square sectional beam profile proper connection methods for the modular rack were devel-oped. Structural units were subsequently realized step by step, while the investigation of stiffness and strength of the profiles was continued. The testing of the structural units under simulated field conditions revealed some minor constructional improvement capabilities. The constructional improvements and the knowledge from the material and profile testing were put into practice in the prototype. Finally sound measurements were carried out to compare several conveyors made of different materials (including the prototype) in respect to the emitted sound level.

La découverte du site Nepress (BiEr-21) en 2004 et les saisons de fouilles subséquentes ont permis de découvrir de nombreux vestiges archéologiques. Ce mémoire a donc pour objectif de déterminer l’identité culturelle des occupants qui ont fréquenté le site, en prenant en considération les activités rituelles et la stratégie d’approvisionnement en matière lithique. Pour y parvenir, une analyse morpho-métrique de l’assemblage lithique a été effectuée. La distribution intra-site des artéfacts a également été prise en considération lors de l’analyse. Une séquence chronologique du Nord-Est américain remontant au Sylvicole inférieur est présentée dans ce mémoire. Une période d’occupation semble dominer sur le site Nepress, soit le Sylvicole inférieur. Cette manifestation est caractérisée par la présence d’artéfacts diagnostiques de la culture Meadowood. Ces objets sont un grattoir triangulaire bifacial Meadowood, ainsi qu’une imitation de pointe de type box-base. / The discovery of the Nepress site (BiEr-21) in 2004 and the subsequent excavations have revealed many archaeological remains. This thesis has seeks to determine the cultural identity of the site’s occupants, taking into account their ritual activities and their lithic procurement strategy. To achieve this, a morpho-metric analysis of the lithic assemblage was undertaken. The intra-site artifact distribution was also taken into account. A chronological sequence for Northeastern North America going back to the Early Woodland is presented. The Early Woodland appears to dominate the occupation of the Nepress site. This is characterised by the presence of diagnostic artifacts of the Meadowood culture. These objects are a triangular bifacial Meadowood scraper, as well as an imitation of a projectile point.