Pålgrundläggning är en vanlig metod vid grundläggning där sämre markförhållanden råder. För broaroch andra anläggningskonstruktioner måste stora laster tas i beaktande, både horisontella ochvertikala. Att dimensionera för detta kan vara en tidskrävande process som kräver många upprepadeberäkningar för att hitta en fungerande pålkonfiguration.Syftet med denna studie är att undersöka hur man med hjälp av ett avancerat analysprogramtillsammans med parametrisk modellering kan effektivisera processen för beräkning av pålgrupper.Studien avser undersöka hur man kan uppnå detta syfte med hjälp av Sofistik, ett FEM-program, isamverkan med Rhinoceros och Grasshopper, program för CAD och visuell programmering. Måletmed studien är att ta fram en effektiv metod där man med hjälp av ett skript med ändringsbaraparametrar kan skapa en mer automatiserad och flexibel process för modellering och beräkning avpålgrupper. Med metoden ska man kunna skapa och beräkna pålkonfigurationer under varierandeförutsättningar, med ett resultat som står sig väl mot mer etablerade metoder.Studien har visat att metoden är användbar för syftet att modellera och beräkna pålgrupper. Arbetet harresulterat i ett skript där användaren kan definiera ingående parametrar om material, geometri ochlaster, och genom skriptet både skapa en tredimensionell modell av en pålgrupp och sedan analyseradenna i analysprogrammet. Processen sker till stor del automatiskt och är flexibel på så sätt attparametrarna kan ändras och direkt uppdatera modellen och utföra en ny beräkning.Metoden har visat sig ge jämförbara resultat med ett etablerat beräkningsprogram för pålar, CAERymdpålgrupp, under förutsättning att en förenklad beräkning görs där bottenplattan ges oändligstyvhet. / Piling is a common method of foundation where poorer soil conditions prevail. For bridges and otherplant structures, large loads must be considered, both horizontal and vertical. Dimensioning for thiscan be a time-consuming process that requires many repeated calculations to find a working pileconfiguration.The purpose of this study is to investigate how, with the help of an advanced analysis programtogether with parametric modelling, the process for calculating pile groups can be streamlined. Thestudy aims to investigate how this goal can be achieved with the help of Sofistik, a FEM program, incollaboration with Rhinoceros and Grasshopper, programs for CAD and visual programming. The aimof the study is to develop an efficient method where, with the help of a script with adjustable values, amore automated and flexible process for modeling and calculating pile groups can be created.The study has shown that the method is useful for the purpose of modeling and calculating pile groups.The work has resulted in a script where the user can define parameters about material, geometry andloads, and through the script create a 3D model of a pile group and analyze this in the analysisprogram. The process is largely automatic and is flexible in such a way that the parameters can bechanged and directly update the model to perform a new calculation.The method has been shown to give comparable results with an established calculation program forpiles, CAE Rymdpålgrupp, provided that a simplified calculation is made where the foundation slab isgiven infinite stiffness.

Det här projektet syftade till att studera och införa ett skript för parametriserad modellering av en bro i programmen Rhinoceros och Grasshopper. Skriptet skapades genom visuella programmeringskomponenter och brokonstruktionen som modellerades i detta projekt var en plattrambro. Parametrisk design är ett verktyg som skapar modeller med hjälp av olika förutsättningar kallade parametrar. Dessa parametrar kan ändras direkt i programmen som även ändrar modellen. Detta underlättar redigeringsprocessen av modeller i projekt samt gör det möjligt för flera variationer av samma konstruktionstyp att hanteras i ett och samma skript. I detta projekt användes även Tekla Structures vilket är ett kraftfullt BIM-verktyg som klarar av att modellera och räkna på hus- och anläggningskonstruktioner. Rhinoceros-Grasshopper kopplas till Tekla med programmets eget plug-in, kallad Tekla live-link. Ett flertal intervjuer har genomförts med experter inom området för input om hur programmen används idag, inom de olika byggföretagen, samt hur långt modellering med parametrar har kommit. Resultatet av detta examensarbete visar att parametriserad modellering främst är användbart vid redigeringar av modeller. Den modellerade konstruktionen programmeras på ett sätt där alla dess ingående delar hänger ihop. Då kan redigeringar på specifika delar göras utan att behöva ändra/modellera de resterande delarna på nytt. Detta gör att mindre arbete behöver läggas på redigeringar. Vid projekt med mindre konstruktioner eller enkla geometrier är program som Tekla ett snabbare och smidigare verktyg än Rhinoceros då programmering av skript kan ta mycket tid. Arbetet som har genomförts i detta examensarbete visar dock att det kan vara lämpligt att använda parametriserad modellering vid projekt med större eller mer komplicerade konstruktioner. / The purpose of this thesis was to study and implement a script for a parametric modelling of a bridge in Rhinoceros 3D Grasshopper. The script was done with visualized programming components and the structure was a slab frame bridge. Parametric design is a tool that create models with the use of different prerequisites called parameters. These parameters can be changed directly in the script which in turn changes the model. This makes the editing process of the model easier and allows for greater variations of the same structure to be handled within the same script. In this project, Tekla Structures was used which is a powerful BIM tool that can be used for design and analyses of various structures. Rhinoceros Grasshopper was connected to Tekla Structures with their own plugin, called Tekla live link. Several interviews have been conducted with experts and experienced people in the subject for input on how the programs are used today in the building branch as well as how far parametric design has come. The results of this thesis shows that parametric design is useful mostly when it comes to redactions of an already existing model. The designed structures are programmed in such a way that all their different components are connected. This allows for edits and redactions to be made on one specific component without having to change or redesign the rest. This saves time during the designing part of a project. Although, when it comes to smaller scaled projects or structures with simple geometries,using programs such as Tekla Structures is still faster and easier as opposed to using Rhinoceros Grasshopper. This is because programming and creating a script can take a lot of time and is very complicated at times. However, the result of this thesis shows that it could be wise to use parametric programming when it comes to larger projects and/or more complicated structures.

The coconut rhinoceros beetle, an economically important pest of coconut and oil palms, is effectively managed by application of its natural pathogen, the Oryctes nudivirus (OrNV), which act as a bioinsecticide. While this approach offers an environment-friendly alternative to chemical pesticides, the current method of production in infected larvae suffers from inconsistencies in virus productivity and purity. While the anchorage-dependent DSIR-HA-1179 insect cell line has been identified as a susceptible and permissive host for OrNV and therefore would be suitable for the in vitro mass production of the virus, no attempts have been made toward the mass production of the virus, because of the technological challenges that working with DSIR-HA-1179 cells represent. Thus, the main objective of this research was to develop processes for the in vitro production of OrNV in the DSIR-HA-1179 cell line. Knowledge of the growth kinetics and metabolic properties of the host cell line in a chosen culture medium, as well as the selection of an appropriate infection strategy, form the basis for the rational development of bioreactor-based virus production processes. However, characterization of these properties in the DSIR-HA-1179 cell line has been virtually precluded, due to its strongly adherent growth characteristics and the lack of a reliable method to accurately dissociate and count cells grown in monolayers. Using TrypLE™ Express enzyme, a technique allowing the precise counting of cells was developed. The cell line was adapted to grow in four serum-supplemented culture media: TC-100, IPL-41, Sf-900 II and Sf-900 III, which were then individually screened for cell growth and virus production in 25 cm2 attached T-flask cultures. TC-100 supplemented with 10% fetal bovine serum was chosen as a suitable culture medium, based on its capacity for achieving a high cell yield and OrNV production. The cell line metabolism was characterized with respect to nutrient consumption and metabolites production in this culture medium. Glucose, along with glutamine were found to be the nutrients that were consumed faster and to a greater extent, while other amino acids were not consumed to a significant degree. The production of metabolites was characterized by non-production of lactate and ammonia, and production of alanine, as a non-toxic alternative to ammonia. The influence of cell density (CD) at time of infection (TOI) and multiplicity of infection (MOI) on OrNV production was evaluated in T-flask cultures that were infected at different CDs at the TOI and a range of MOIs. The CD at TOI was found to significantly influence OrNV yields, while MOI influenced the dynamics of infection. The cell density effect was found to exist for the DSIR-HA-1179/OrNV system with the progressive decline in cell-specific yield beginning at low cell densities. It was found that in order to maximize OrNV volumetric yield, a combination of MOI and CD at TOI should be selected that allows to keep the maximum cell density reached by the infected culture within a range between 5.0 and 7.0 x 105 viable cells/ml. The roller bottle system was evaluated for its potential to scale-up DSIR-HA-1179 cell growth and OrNV production, and culture parameters were optimized for the improvement of cell and virus yields. An inoculum density of 3.3 x 105 cells/ml and culture volume of 60 ml resulted in the highest cell yield of 1.5 x 106 cells/ml, in 490 cm2 roller bottles. It was found that an optimal infection strategy for roller bottle cultures, which represented the most efficient use of viral inoculum, involved infecting cells at a density of 5.0 x 105 cells/ml and at a MOI of 1. The resulting OrNV volumetric yield of 2.5 x108 TCID50/ml, improved significantly the viral yields obtained in attached T-flask cultures infected under similar conditions (6.8 x 107 TCID50/ml). The microcarrier system was also evaluated for culturing DSIR-HA-1179 cells and producing OrNV in spinner flask bioreactors. Three types of microcarriers (Cytodex-1, Cytodex-3 and Cultispher-G microcarriers) were screened for their ability to support DSIR-HA-1179 growth. Cells attached to Cytodex-1 and 3, but failed to attach to Cultispher-G microcarriers. The final cell density reached in microcarrier culture was dependent on bead type and concentration, and the cell to bead ratio. At an optimal bead concentration of 1 mg/ml and cell to bead ratio of 30, cells grew to a maximum density of 1.7 x 106 cells/ml on Cytodex-1, but only to 1.3 x 106 cells/ml on Cytodex-3 microcarriers. Since it supported higher cell yields, Cytodex-1 was chosen to study the kinetics of OrNV production in this system. Microcarrier cultures infected at a cell density of 5.0 x 105 cells/ml and a MOI of 1, produced OrNV at 1.4 x 108 TCID50/ml, which was higher than the yield obtained in T-flask cultures infected under similar conditions. A framework of knowledge on the physiology, metabolism and growth kinetics of the DSIR-HA-1179 insect cell line has been developed in this thesis. In addition, the feasibility of using roller bottles and microcarrier systems for the in vitro production of the virus has been ascertained. It is envisaged that these findings will contribute to the future development of a large-scale industrial process for the production of the OrNV biopesticide.

Thesis (Ph.D.)--Ohio University, March, 2009. / Title from PDF t.p. Includes bibliographical references.

Michalík, P. Animated interactive 3D model parts of computer. Diploma thesis. Brno: Mendel University in Brno, 2015. This diploma thesis describes possibilities of creation, use and presentation three-dimensional models, selection of a suitable solution for creation and presentation three-dimensional models, creating static three-dimensional models of computer components and their completion of animation and interactive elements to these models.

The re-introduction of black rhinoceros onto the Great Fish River Reserve in the Eastern Cape, following the species absence for over a century, created an opportunity to study this species in the thicket biome, and in particular the succulent thicket of the Great Fish River valley. The thicket biome is quite different from the habitats in which other extant populations of these animals are found and where studies of their demography have been undertaken. Data collection occurred from May 1986, with the arrival of the first four animals to December 2008, when the population was estimated to be 144 individuals. A variety of monitoring techniques were used including direct ground observations, aerial helicopter census, aerial monitoring by microlight aircraft, camera traps and opportunistic observations, each with its own advantages and disadvantages. The combination of methods and effort has resulted in the population being recognized as completely known with up to 97% of the animals being located at least once per year with a mean frequency of sightings of 11.5 per animal per year. In 2008, the population was about 20% juvenile, 36% sub-adult and 44% adult with a female biased sex ratio of 1.3 females per male. Mean age at first birth was about 80 months and this increased significantly with increasing density of black rhinoceroses. Mean intercalf interval was about 28 months and 41% of adult females gave birth each year. Mortality rates were low for juveniles, higher for sub-adults and higher for males than females. There was a weak but significant positive effect of density on male mortality. Population growth rate was about 10% and was not significantly affected by density. These results suggest that this population is beginning to show the first indications of density dependant constraints and that harvesting should be implemented in order to maintain the productive tempo. A secondary goal of the Management Plan will then be realized as the population functions as a donor to establish founder populations elsewhere within the Diceros bicornis minor range.

Capturing and holding of white rhinoceros (Ceratotherium simum) for the reintroduction to new reserves or breeding in zoos often involves a risk of mortality. Non-invasive techniques to monitor the stress experienced by these animals may guide the selection of management techniques that reduce risks to animal well-being. The aim of the study was to evaluate the biological relevance of a developed technique to monitor stress hormone metabolites in faecal samples of wild-caught and captive-bred white rhinoceros. Faecal corticosterone concentrations were measured via radioimmunoassay (125I RIA), in seven white rhinoceros (3 males and 4 females), at three sites, before and after an adrenocorticotropic hormone (ACTH) challenge test and control saline injection. Administration of ACTH resulted in a significant increase in faecal corticosterone concentrations (up to 350% above pre-ACTH baseline) within 1-2 days of injection, returning to baseline 4 days post-injection. It was found that individual baseline corticosterone concentrations fluctuate naturally and vary between individual animals, suggesting that an adequate baseline period of faecal sampling is needed in order to accurately assess responses to ACTH stimulation. Furthermore, the technique proved sensitive enough to detect elevations in faecal corticosterone concentrations due to environmental stressors. Data of faecal corticoid concentrations were correlated with gastrointestinal transit (GIT) times before and after ACTH and saline treatment by using art glitter as a digestive marker. This showed that gut passage times correlated to the ACTH-induced time to peak. Overall the results confirm that measurements of faecal corticosterone metabolites with the validated 125I RIA is a useful diagnostic tool to monitor adrenocortical activity in white rhinoceros. This study can therefore provide a methodology for examining chronically heightened adrenal activity in these animals and consequently be used to inform management strategies that aim to improve the welfare of white rhinoceros in captivity. / Dissertation (MSc)--University of Pretoria, 2011. / Anatomy and Physiology / unrestricted

The endeavor for optimization and development in the construction industry is warranted where new solutions are highly sought for. The importance of communication and efficiency in a project planning phase is of highest importance. The objective of this study is to highlight the advantages and disadvantages of a parameter-driven modeling method, by using a Grasshopper software. To explore the possibilities of the software, a script will be created. The script will contain a parallel framework also known as Warren’s framework. In this study, a survey is sent for applicable practitioners in the construction industry where the purpose is to receive information about the industry's opinion about the subject. A parameter-driven modeling method refers to software such as Grasshopper and Dynamo. The software is node-based unlike traditional modeling methods based on manual line drawing. There is no fixed geometry in a parameter-driven modeling software. Therefore, in order to illustrate models, different visualization platforms like Rhino are needed. A considerable amount of time has been spent creating a script in Grasshopper where a user with limited experience will naturally face complications among the way. Many of the survey participants considered that the main con of parametric modeling is the amount of time allocated to creating a script. Thought when the script is completed, changing parameters to influence the model outcome is uncomplicated. Additionally, there is potential regarding time efficiency since the software eliminates all need to perform repetitive tasks. Parametric modeling is a flexible form of work where changes later in a stage are always available.

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This diploma thesis is focused on the design of a product family of portable veterinary x-ray generators. The thesis deals with the possibilities of using parameterization when designing products which are part of a product series. The main body of the thesis lies in the design of a parametric script which generates variations of portable veterinary x-ray generators depending on the size and position of the inner components. The outer surfaces are defined by algorithms from the input parameters. It is possible to alter the individual attributes (e.g. the progresion of curves, the dimensions of component parts and the proportions between the individual parts). The output from the parametric script is three size-variations of the product.