This study is to develop the manufacturing processes of thin film bulk acoustic device by MEMS technology, including lithography, wet etching, dry etching and rf Magnetron sputtering.LPCVD is used to deposit SiNx as the membranes and mask for etching of silicon wafer. The electrodes of molybdenum metal (Mo) and piezoelectric layer of aluminum nitride (AlN) on top side are prepared by dc and rf sputtering,respectively. The acoustic cavity on back side is achieved by 30%wt 100¢J KOH solution and reactive ion etching (RIE). In this study, the crystallography of the coated films was analyzed by X-ray diffraction. The surface and cross-sectional morphologies of AlN films were investigated by electron microscope. The piezoelectric layer of AlN thin film prepared by rf magnetron sputtering shows the highly c-axis preferred orientation and fine morphology under the optimal sputtering parameters of rf power of 200W, sputtering pressure of 3 mTorr, substrate temperature of 400¢J and nitrogen concentration of 25%. The frequency responses of fabricated FBAR devices are evaluated using the Hewlett-Packard 8720-ET network analyzer. Moreover, the optimal thickness of 1500Å SiNx film prepared by LPCVD revealed the excellent masking effect and non-stress for membrane. The yield for the fabrication of acoustic cavity is maximum of 85% can be achieved by using the combined etching steps of wet and dry etching.

No description available.

Bakgrund: Förmågan att kunna lokalisera ljud är en binaural process som försvåras i samband med hörselnedsättning, eftersom tillgången till akustiska ledtrådar begränsas. Hörapparattillverkare strävar efter att främja användarnas tillgång till dessa ledtrådar genom nya innovationer. Den vanligaste modellen av hörapparat är i dagsläget bakom-örat-apparaten med högtalarenhet i hörselgången (RIE). Modellens mikrofonplacering bakom örat tillåter inte användaren att utnyttja öronmusslans akustiska egenskaper som hjälper vid ljudlokalisation. En hörapparattillverkare har därför tagit fram en ny teknik (M&RIE) som implementerar en ytterligare mikrofon placerad i hörselgången som bland annat syftar till att gynna användarens förmåga att lokalisera ljud. Syftet med denna studie är att undersöka ljudlokalisationsförmågan i horisontalplan med de två högtalarenheterna “M&RIE” och “RIE” hos normalhörande vuxna. Metod: 18 normalhörande deltagare genomförde tre lokalisationstest med respektive teknik. Signalerna i testen bestod av ett pulserande brus, en biltuta samt en ringklocka. Resultat: RIE presterade generellt bättre än M&RIE i lokalisationstestet vid samtliga signaler. En mindre andel bak-fram-förväxlingar skedde vid lokalisation med RIE än med M&RIE. Slutsatser: Den begränsande faktorn att M&RIE behöver anpassas med täta domer leder till nackdel i lokalisationsförmåga vid anpassning för mild hörselnedsättning. Undersökningen indikerar att fördelen av en öppen anpassning hos RIE är större än fördelen av en extra mikrofon i hörselgången för normalhörande deltagare vid horisontell ljudlokalisation. / Background: The ability to localize sounds is a binaural process that is limited by hearing loss due to a decreased access to acoustic cues. Hearing aid manufacturers strive to promote users' access to these cues through new innovations. Currently, the most common type of hearing aid is the behind-the-ear device with the speaker unit located in the ear canal (RIE). The model's microphone placement behind the ear does not allow the user to take advantage of the acoustic properties of the pinna, which aid sound localization. Therefore, a hearing aid manufacturer has developed a new technology (M&RIE) that implements an additional microphone placed in the ear canal, which aims to benefit its users' ability to locate sounds. Aim: The aim of this study is to investigate the ability to locate sound in the horizontal plane using the two speaker units "M&RIE" and "RIE" in normal-hearing adults. Methods: 18 normal-hearing participants performed three localization tests with each technology. The signals in the tests consisted of a pulsating noise, a car horn, and a bicycle bell. Results: RIE generally outperformed M&RIE in the localization test for all signals. A lower percentage of back-front-confusions occurred in localization with RIE than with M&RIE. Conclusions: The limiting factor that M&RIE must be fitted with occluded domes leads to a disadvantage in the localization ability when fitted for mild hearing loss. The study indicates that the advantage of an open-fit in RIE is greater than the advantage of an extra microphone in the ear canal for normal-hearing participants in horizontal sound localization.

Made available in DSpace on 2014-12-17T15:31:00Z (GMT). No. of bitstreams: 1 GuilhermeMC.pdf: 675094 bytes, checksum: 480716331db06f40e13bac10b74ea070 (MD5) Previous issue date: 2006-05-19 / Esse estudo se prop?s a avaliar um m?todo auxiliar de diagn?stico (DIAGNOdent?) na predi??o de c?rie de dentina em dentes dec?duos, atrav?s de valida??o histol?gica e de microdureza. Verificando poss?veis diferen?as entre os valores obtidos atrav?s do m?todo auxiliar, al?m de determinar um ponto de corte para parada de remo??o de tecido cariado. A amostra do estudo foi de 15 crian?as que apresentaram 21 cavidades de c?rie, tratadas e analisadas desde antes da realiza??o da restaura??o at? quando da exodontia do elemento dent?rio. Os esp?cimes foram analisados atrav?s do DIAGNOdent? antes da abertura das cavidades, ap?s a remo??o do tecido cariado e depois da reabertura das cavidades que se deu ap?s a exodontia dos elementos dent?rios. Posteriormente, receberam preparo metalogr?fico para realiza??o de testes de microdureza e microscopia ?ptica que serviram como forma de valida??o para as mensura??es obtidas pela fluoresc?ncia a laser. Houve diferen?a significativa entre os valores DIAGNOdent? encontrados antes da abertura da cavidade e os demais (p < 0,0001). Havendo correla??o significativa (r = 0,432 ; p = 0,019) apenas para as aferi??es obtidas antes da abertura das cavidades e quando do t?rmino da remo??o do tecido cariado. Para a microdureza, as m?dias axiais foram significativamente maiores que as pulpares, existindo correla??o entre a microdureza pulpar e os valores do DIAGNOdent? ap?s a reabertura (r = - 0,472 ; p = 0,002). Valores de 15,38% para a sensibilidade, 100% para a especificidade, 100% para o valor preditivo positivo e 71,79% para o valor preditivo negativo, foram obtidos quando se utilizou um ponto de corte de 30 para fluoresc?ncia a laser, tendo como padr?o-ouro a microdureza pulpar. Partindo da m?dia dos valores de fluoresc?ncia a laser obtidos ap?s a remo??o do tecido cariado e acrescentando-se um desvio-padr?o a esta, o m?todo indicou o valor de 19 como ponto de corte para cessar a remo??o de dentina. Concluiu-se que, nas condi??es analisadas, o m?todo auxiliar de diagn?stico (DIAGNOdent?) ? um m?todo acurado na predi??o de c?rie de dentina em dentes dec?duos. Al?m disso, o m?todo comprovou que o padr?o usual de remo??o de dentina garante a remo??o do tecido cariado

O trabalho teve como objetivo avaliar a concentração sérica de estrógeno e progesterona em cadelas durante o ciclo estral por imunoensaio multianalito (Luminex® xMAP®), visando avaliar a aplicabilidade fisiológica e comparar os resultados obtidos com os valores já estabelecidos na literatura pelo método de radioimunoensaio (RIE). Foram utilizadas 7 cadelas da raça Border Collie, residentes do Canil Espaço Ducão (Valinhos/SP) de diferentes idades e ao primeiro sinal de sangramento vulvar (proestro/estro) foram coletadas amostras a cada 48 hs e ao seu termino (diestro), foi realizado coletas semanalmente (intervalo de 7 dias) durante aproximadamente 3 semanas. Houve correlação positiva apenas entre os valores de progesterona na fase de proestro/estro (p=0,001). Apesar de não ter ocorrido correlação entre as outras fases, as medias dos valores séricos de estradiol e progesterona foram estatisticamente significantes, em ambas as fases, sendo os valores obtidos pelo Luminex® maiores que os obtidos pelo RIE. Os valores de progesterona confirmaram os dados fisiológicos esperados em ambas as metodologias, porém os valores de estradiol não foram compatíveis com o esperado fisiologicamente, exceto quando analisados a partir das medias globais em ambas as fases. Novos estudos de validação laboratorial serão necessários para confirmar a validação fisiológica, principalmente no perfil da progesterona. Podemos concluir ainda que os valores de referência para o Luminex® devem ser diferentes dos valores já estabelecidos pelo RIE para ambos os hormônios testados / The study aimed to evaluate the serum concentration of estrogen and progesterone in female dogs during the estrous cycle by multiplex immunoassay (Luminex® xMAP®), to evaluate the physiological applicability and compare the results with the values established in the literature by radioimmunoassay (RIA). We used seven Border Collie bitches, residents in Kennel Espaço Ducão (Valinhos/SP), of different ages and for the first sign of vulvar bleeding (proestrus/estrus) samples were collected every 48 hours and its end (diestrus) were collected weekly (every 7 days) for about three weeks. There was a positive correlation only between progesterone values in the proestrus/estrus phase (p = 0.001). Despite having not occurred correlation between the other phases, the median of serum estradiol and progesterone values were statistically significant in both phases, the values obtained by Luminex® is higher than by RIA. Progesterone values confirmed the expected physiological data in both methods, however estradiol values were not physiologically compatible with the expected, except when analyzed from the global mean in both phases. A laboratory validation studies will be needed to confirm the physiological validation, especially in progesterone profile, and we can conclude that the reference values for the Luminex® should be different from the values established by RIE for both hormones tested

In this master thesis, the goal was to devise design patterns and a fabrication processfor manufacturing a 3-D rotationally symmetric converging-diverging cold gasmicronozzle in silicon.The report explains the theory of etching and the methods involved. The work beginswith calculations and simulations of the etching processes. The chosen etch techniqueutilizes so called microloading and reactive ion etching lag effects, which essentially arephenomena where the etch rate can be adjusted by breaking up mask features intosubpatterns, and the etch depth for a given recipe and time can be made to differlocally. The subpatterns consisted of very small rectangles and triangles withalternating concentration. Five different recipes for the reactive ion etching weretried, where the coil power, platen power, pressure, temperature and time wasvaried.Etch rates could be made to differ locally depending on the concentration ofsubpatterns within the mask feature. The etch rates were also affected by the recipeparameters such as coil power, platen power, and pressure. High coil and platenpower increased the etch rate, while high pressure reduced the etch rate. The platenpower also affected the surface roughness.A solution for reducing misalignment problems in the future for the fusion bondingprocess resulted in the proposed moiré patterns that were made to showmisalignments down to 0.2 μm.Through scanning electron microscopy, the Nozzle 5_4_2 was concluded to have themost rotationally symmetric cross section at both the throat and the outlet. It hasthroat diameter of 31.1 μm with a depth of 34.2 μm and an outlet diameter of146.4 μm with a depth of 113.2 μm

La pr??sente ??tude analyse d???une perspective pragmatique le journal de prison de Daniel Timsit. Le but de notre approche pragmatique est de d??finir le genre du journal intime, et d???observer si le texte de Timsit correspond aux attentes du lecteur. C???est ainsi que la premi??re partie de notre premier chapitre est consacr??e aux caract??ristiques g??n??riques de l???autobiographie et du journal intime. De l???analyse du deuxi??me et du troisi??me chapitre, il ressort que dans le journal de Timsit quelques-uns de ces principes ne sont pas respect??s : le rejet de la fiction et de la po??tique, la calendarit??, la simultan??it?? et l???insouciance de la beaut?? du style. Dans le deuxi??me chapitre, l???analyse narrative r??v??le un narrateur qui ??crit son journal au jour le jour en prison et un narrateur qui a une perspective r??trospective. Cette structure entra??ne la division du r??cit : d???un c??t??, il y a le journal intime, de l???autre, l???autobiographie. Le contraste entre les deux types de discours est mis en ??vidence par l???analyse de la simultan??it?? au niveau des d??ictiques. La narration h??t??rodi??g??tique introduit un troisi??me type de r??cit, qui rel??ve de la fiction. Dans le troisi??me chapitre, l???analyse des d??ictiques fait ressortir l???intrusion de traits fictionnels dans le journal proprement dit, par le biais des r??flexions projet??es au-del?? de l???exp??rience carc??rale. C???est toujours dans le troisi??me chapitre que nous estimons que la deuxi??me et la troisi??me personne du singulier repr??sentent des marques de la subjectivit?? dans le journal intime, tout comme la premi??re personne. Le pronom nous exprime la subjectivit?? int??gr??e dans la collectivit?? des prisonniers. L???approche pragmatique nous aide aussi ?? examiner le contexte plus large, le contexte social, politique et ??conomique, qui fait que cet ouvrage ne correspond pas aux attentes cr????es par les conventions formelles.

Submitted by Nivaldo Melo (nivaldo.melo@ufvjm.edu.br) on 2015-11-27T22:07:18Z No. of bitstreams: 2 bruna_mota_alencar.pdf: 1299407 bytes, checksum: 68d1fc673481812ec5ce638730779149 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-12-02T11:25:15Z (GMT) No. of bitstreams: 2 bruna_mota_alencar.pdf: 1299407 bytes, checksum: 68d1fc673481812ec5ce638730779149 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Made available in DSpace on 2015-12-02T11:25:16Z (GMT). No. of bitstreams: 2 bruna_mota_alencar.pdf: 1299407 bytes, checksum: 68d1fc673481812ec5ce638730779149 (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2015 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A qualidade emocional das rela??es familiares pode afetar os comportamentos de sa?de bucal. Os estudos que investigaram fatores familiares associados ? sa?de bucal n?o aprofundaram em quest?es psicossociais. A compreens?o dessa associa??o pode indicar os caminhos para promo??o de sa?de, enfatizando a necessidade de a??es multidisciplinares, melhorando, assim, a sa?de bucal da popula??o. Particularmente em crian?as pr?-escolares, que dependem do cuidado de sua fam?lia, a investiga??o da influ?ncia de fatores psicossociais familiares sobre a preval?ncia de doen?a bucal ? importante. Assim, o objetivo desse estudo foi avaliar a influ?ncia da coes?o/adaptabilidade familiar, de fatores sociodemogr?ficos e de comportamentos relacionados ? sa?de bucal sobre a preval?ncia de c?rie dent?ria n?o tratada em crian?as pr?-escolares. Para isso, uma amostra representativa de 461 crian?as foi recrutada em creches e pr?-escolas na cidade de Diamantina, Brasil. A presen?a de c?rie n?o tratada foi detectada pelos crit?rios do Sistema Internacional de Avalia??o e Detec??o de C?rie (International Caries Detection and Assessment System- ICDAS II). Informa??es sociodemogr?ficas, tais como caracter?sticas da crian?a, escolaridade materna, renda e n?mero de pessoas que vivem da renda, bem como informa??es relacionadas a comportamentos de sa?de bucal tamb?m foram investigadas. A coes?o/adaptabilidade familiar foi investigada por meio do Family Adaptability and Cohesion Evaluation Scale (FACES III) que classifica as fam?lias, de acordo com o seu funcionamento, em balanceadas, moderadamente balanceadas e n?o balanceadas. An?lise descritiva e modelo de regress?o de Poisson foram utilizados para an?lise dos dados. A preval?ncia de c?rie n?o tratada foi de 30,6%. A An?lise multivariada mostrou que crian?as que pertenciam a fam?lias n?o balanceadas, classificadas de acordo com o FACES III, tinham maior preval?ncia de les?es n?o tratadas de c?rie dent?ria (RP: 1,49; IC95%: 1,01-2,20; p= 0.045). Al?m disso, rendas familiares de dois a menos de cinco sal?rios m?nimos ( RP: 2,80; IC95%: 1,16-6,75; p=0 .021), de um a menos de dois sal?rios m?nimos (RP: 4,15; IC95%: 1,73-9,85; p= 0.001) e menor do que um sal?rio m?nimo (RP: 4,07; IC95%: 1,65-10,00; p= 0.002) tamb?m foram associadas com a presen?a de les?es n?o tratadas de c?rie dent?ria em crian?as pr?-escolares. Concluiu-se que crian?as pr?-escolares de fam?lias n?o balanceadas tinham maior preval?ncia de c?rie dent?ria n?o tratada. Al?m disso, menores rendas familiares foram fatores de risco para a presen?a de c?rie n?o tratada em crian?as pr?-escolares. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Odontologia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / ABSTRACT The emotional quality of family relationships can affect the behaviors related to oral health. Studies investigating family factors associated with oral health have not deepened in the psychosocial issues. The understanding of this association can indicate the pathways to health promotion, emphasizing the necessity of multidisciplinary actions to improve oral health in the population. Especially in preschool children, which depend on the care of their family, the investigation of family-related psychosocial factors and the occurrence of oral disease is important. Thus, the objective of this study is to evaluate the influence of familiar cohesion/adaptability, sociodemographic factors and of behaviors related to oral heath on the prevalence of untreated dental caries in preschool children. For this, a representative sample of 461 children were recruited in day care centre/preschools in the city of Diamantina, Brazil. The presence of untreated caries were detected by the International Caries Detection and Assessment System (ICDAS II) criteria. Sociodemografic information, such as children characteristics, maternal level of education, income and number of people living of the same income, as well as information related to oral health behavior were also investigated. The family cohesion/adaptability was investigated by the Family Adaptability and Cohesion Evaluation Scale (FACES III) which classifies the families according to their functioning, in balanced, moderately balanced, and not balanced. Descriptive analyses and Poisson regression models were used to analyze the data. The prevalence of untreated dental caries was of 30,6%. The multivariate analyses showed that children belonged to unbalanced families, classified according to FACES, had a greater frequency of untreated dental caries (1,01-2,20; p=0.045). Furthermore, family income from 2 to less than 5 minimum salaries (RP:2,80; IC 95%:1,16-6,75; p=0.021), from one to two minimum salaries (RP: 4,15; IC95%: 1,73-9,85; p= 0.001) and less than one minimum salary (RP: 4,07; IC95%: 1,65-10,00; p= 0.002) were also associated with the presence of untreated caries lesions in preschool children. In conclusion, preschool children of unbalanced families had greater untreated dental caries prevalence. Futhermore, smaller family incomes were pointed as risk factors for presence of untreated caries in preschool children. Keywords: dental caries, preschools children, family functioning.

?rea de concentra??o: Odontopediatria. / Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-12-22T13:35:04Z No. of bitstreams: 2 izabella_barbosa_fernandes.pdf: 2088859 bytes, checksum: 015e8cf831539e0c08c0656e3ff8e95e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-12-22T13:35:37Z (GMT) No. of bitstreams: 2 izabella_barbosa_fernandes.pdf: 2088859 bytes, checksum: 015e8cf831539e0c08c0656e3ff8e95e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2015-12-22T13:35:37Z (GMT). No. of bitstreams: 2 izabella_barbosa_fernandes.pdf: 2088859 bytes, checksum: 015e8cf831539e0c08c0656e3ff8e95e (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2014 / A c?rie dent?ria n?o tratada ? a condi??o bucal mais frequentemente associada com um impacto negativo na qualidade de vida de crian?as por estar associada ? dor, desconforto, dificuldades na mastiga??o, problemas de fala, dist?rbios no sono, problemas psicol?gicos, dificuldades na escola e dificuldades na intera??o social da crian?a. Essa associa??o tem sido frequentemente verificada em adultos, adolescentes, crian?as escolares e pr?-escolares. A associa??o de impacto da c?rie dent?ria em beb?s sobre a qualidade de vida ? ainda desconhecida. O objetivo deste estudo foi avaliar o impacto de diferentes est?gios de progress?o da c?rie dent?ria na qualidade de vida de beb?s e de suas fam?lias na cidade de Diamantina, Minas Gerais. Uma amostra de 308 beb?s de 1 a 3 anos de idade foi submetida a um exame cl?nico para avaliar a c?rie dent?ria atrav?s do Sistema Internacional de Avalia??o e Detec??o de C?rie - International Caries Detection and Assessment System (ICDAS). As m?es dos beb?s foram convidadas a responder dois question?rios: um sobre qualidade de vida relacionada ? sa?de bucal (QVRSB) das crian?as e de suas fam?lias, o Brazilian Early Childhood Oral Health Impact Scale (B-ECOHIS) e outro sobre as caracter?sticas e condi??es sociodemogr?ficas dos beb?s e de suas fam?lias. A an?lise estat?stica foi realizada atrav?s do programa SPSS - Statistical Package for Social Sciences (SPSS for Windows, version 20.0, SPSS Inc. Chicago, IL, USA) e envolveu an?lise descritiva, os testes Kappa, Kruskal Wallis, Mann-Whitney e modelos de regress?o de Poisson hierarquicamente ajustados. A preval?ncia de c?rie n?o tratada foi de 64,3%. A maioria dos beb?s com c?rie apresentava c?rie em seu est?gio severo de progress?o (53,5%). Houve uma diferen?a significativa entre o est?gio de progress?o da c?rie e QVRSB no beb? e na fam?lia. O est?gio severo de c?rie dent?ria [RP IC95%= 2.80 (1.90-4.12)] e a menor idade da m?e [RP IC95%= 1.69 (1.27-2.25) foram associados com o maior impacto na qualidade de vida. Concluiu-se que les?es de c?rie em est?gios mais avan?ados de progress?o foram associados a uma pior qualidade de vida em beb?s e em suas fam?lias. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014. / ABSTRACT Untreated dental caries is the most often oral condition associated with negative impact on quality of life in children by being associated with pain, discomfort, difficulty in chewing, speech problems, sleep disorders, psychological problems, difficulties in school and social interaction of the child. This association has been frequently seen in adults, adolescents, school children and preschoolers. The impact of dental caries in the quality of life of babies still unknown. The aim of this study was to evaluate the impact of different stages of progression of dental caries in the quality of life of babies and their families in Diamantina, Minas Gerais. A sample of 308 babies aged 1-3 years old underwent a clinical examination to assess dental caries through the International Caries Detection and Assessment System (ICDAS). Parents of babies were asked to answer two questionnaires: one about oral health related quality of life (OHRQoL) of children and their families using the Brazilian Early Childhood Oral Health Impact Scale (B-ECOHIS) and another about sociodemographic characteristics and conditions of babies and their families. Statistical analysis was performed using SPSS - Statistical Package for Social Sciences (SPSS for Windows, version 20.0, SPSS Inc. Chicago, IL, USA) and involved descriptive analysis, Kappa, Kruskal-Wallis and Mann-Whitney test and Poisson model regression adjusted hierarchically. The prevalence of untreated dental caries was 64.3%. Most babies with caries had caries in severe progression stage (53.5%). There was a significant difference between the stage of caries progression and OHRQoL on babies and their families. Severe stage of dental caries [OR = 2.80 95% CI (1.90-4.12)] and lower mother's age [OR = 1.69 95% CI (1:27 to 2:25) were associated with the greatest impact on quality of life. It was concluded that dental caries in more advanced stages of progression were associated with worse quality of life in babies and their families.

O trabalho teve como objetivo avaliar a concentração sérica de estrógeno e progesterona em cadelas durante o ciclo estral por imunoensaio multianalito (Luminex® xMAP®), visando avaliar a aplicabilidade fisiológica e comparar os resultados obtidos com os valores já estabelecidos na literatura pelo método de radioimunoensaio (RIE). Foram utilizadas 7 cadelas da raça Border Collie, residentes do Canil Espaço Ducão (Valinhos/SP) de diferentes idades e ao primeiro sinal de sangramento vulvar (proestro/estro) foram coletadas amostras a cada 48 hs e ao seu termino (diestro), foi realizado coletas semanalmente (intervalo de 7 dias) durante aproximadamente 3 semanas. Houve correlação positiva apenas entre os valores de progesterona na fase de proestro/estro (p=0,001). Apesar de não ter ocorrido correlação entre as outras fases, as medias dos valores séricos de estradiol e progesterona foram estatisticamente significantes, em ambas as fases, sendo os valores obtidos pelo Luminex® maiores que os obtidos pelo RIE. Os valores de progesterona confirmaram os dados fisiológicos esperados em ambas as metodologias, porém os valores de estradiol não foram compatíveis com o esperado fisiologicamente, exceto quando analisados a partir das medias globais em ambas as fases. Novos estudos de validação laboratorial serão necessários para confirmar a validação fisiológica, principalmente no perfil da progesterona. Podemos concluir ainda que os valores de referência para o Luminex® devem ser diferentes dos valores já estabelecidos pelo RIE para ambos os hormônios testados / The study aimed to evaluate the serum concentration of estrogen and progesterone in female dogs during the estrous cycle by multiplex immunoassay (Luminex® xMAP®), to evaluate the physiological applicability and compare the results with the values established in the literature by radioimmunoassay (RIA). We used seven Border Collie bitches, residents in Kennel Espaço Ducão (Valinhos/SP), of different ages and for the first sign of vulvar bleeding (proestrus/estrus) samples were collected every 48 hours and its end (diestrus) were collected weekly (every 7 days) for about three weeks. There was a positive correlation only between progesterone values in the proestrus/estrus phase (p = 0.001). Despite having not occurred correlation between the other phases, the median of serum estradiol and progesterone values were statistically significant in both phases, the values obtained by Luminex® is higher than by RIA. Progesterone values confirmed the expected physiological data in both methods, however estradiol values were not physiologically compatible with the expected, except when analyzed from the global mean in both phases. A laboratory validation studies will be needed to confirm the physiological validation, especially in progesterone profile, and we can conclude that the reference values for the Luminex® should be different from the values established by RIE for both hormones tested