The goal of this diploma thesis was to create a new marketing strategy for the chosen company. The company is seated in Prague and has been in the coffee industry business since 2003. The thesis was written to fulfill academic standards and to be a practical guide for the company itself. The first part of the thesis is the theoretical part containing theoretical principals of company strategy, situational analysis and marketing mix. In the second part we applied theory, real data, internal company workshops and our own survey to create a marketing strategy. The goal of the diploma thesis was fulfilled by creating a marketing strategy based on the company´s identity and values, carrying out a situational and market analysis and creation of a marketing mix.

The coffee consumption in Asia has experienced an exponential growth in recent years, it's a combination of a world trend that has embraced coffee drinking culture and elevated it to a Craft level drink, as well as the influence of western cultures. We can observe a sustained growth in Taiwan since in the last 5 years the composition of imports reflect a constant shift to import better and higher quality coffees. In recent years the main cities of Taiwan have seen an increased amount of specialty coffee shop options that focus and growing interest and trend of consumers that have made coffee their daily beverage of choice. As a result Taiwan is now a renowned location because of its talented coffee people that have won in recent years international competition in all of the different categories. We can observe that the market has been segregated in two categories, the high end with Starbucks as the leader in its category, followed by many independent coffee shops that offer high end specialty coffee. In the lower end we have low priced coffee options offered in Chain store coffee shops and convenience stores. The average price of the high end is double the price of the low end options, creating a gap and a market that is currently not being addressed in the market. To be able to differentiate from the low end price options in the market and attract the right consumers to our stores, we will carefully curate our offerings based on market research to determine the right positive price and quality ratio for our consumers. Our product offering will include: Coffee drinks, light pre¬prepared food, as well as roasted coffee and coffee brewing accessories. Our Coffee Shops will create a unique coffee experience that engages the 5 human senses (sight, smell, touch, listening and taste), of the consumer when they enter our strategically located store in a high traffic area, where we will serve on-the¬go as well as 10 sitting tables of 3 people. We will hire coffee passionate consumer oriented Baristas that will familiarize and educate our consumer with the different coffee options available, while we prepare the coffee using innovative coffee preparation methods. With a initial investment of NTD$2,407,000, we expect to have a gross margin of 38%, while serving 450 consuming customers per month at the end of the first year, be cash positive in the 8th month, and be able to return our investment in the middle of the 2nd year.

O café, rico em substâncias bioativas, está entre os maiores contribuintes para a ingestão de antioxidantes em vários países. O tipo de torra dos grãos influencia em sua atividade antioxidante. Estudos indicam que o consumo moderado de café filtrado está envolvido na redução do risco de doenças crônicas não-transmissíveis, geralmente associadas entre si e que se constituem em graves problemas de saúde pública. Entretanto, a literatura não apresenta consenso sobre a ação benéfica do café na redução do risco destas doenças. Objetivos: Comparar a atividade antioxidante de dois graus de torras de café (torra média-clara e média) e seus efeitos sobre biomarcadores de risco cardiovascular em indivíduos saudáveis. Métodos: A caracterização de antioxidantes nas bebidas foi realizada pelas análises de compostos fenólicos totais, perfil de ácidos fenólicos, cafeína, melanoidinas e capacidade antioxidante total - TAC (sequestro do radical DPPH e capacidade de absorbância do radical oxigênio - ORAC). Após 1 semana de washout, vinte voluntários saudáveis (20 a 65 anos) ingeriram café filtrado preparado com torra média-clara ou torra média por 4 semanas e com o outro tipo de torra por mais 4 semanas em um ensaio clínico randomizado do tipo crossover, o qual durou 9 semanas. Lipídeos plasmáticos, lipoproteína (a), homocisteína total, biomarcadores glicêmicos e pressão arterial de 24 horas foram medidos antes do período de intervenção a após a ingestão de cada torra. A atividade antioxidante foi avaliada no plasma e em eritrócitos respectivamente pela TAC (kit Total Antioxidant Status e ORAC) e da atividade das enzimas antioxidantes (superóxido dismutase - SOD, glutationa peroxidase - GPx e catalase - CAT). A capacidade de inibição da peroxidação lipídica foi avaliada no plasma pelas determinações de lipoproteínas de baixa densidade (LDL) oxidadas e 8-isoprostano. Biomarcadores inflamatórios relacionados à disfunção endotelial foram medidos no plasma por imunoensaios. Resultados: Vinte voluntários saudáveis (49,5 + 8,9 anos) foram avaliados. A torra média-clara apresentou maior teor de ácidos clorogênicos (334 mg/150 mL; p < 0,001) e menor teor de cafeína (231 mg/150 mL; p = 0,003) que a torra média (210 mg/150 mL e 244 mg/150 mL, respectivamente). Os teores de melanoidinas foram significamente maiores na torra média (p < 0,001). A TAC não diferiu entre as bebidas. A ingestão de ambas as torras causou aumento nas concentrações de colesterol total e LDL (10 e 12 por cento para a torra média-clara; 12 por cento e 14 por cento para a torra média) (p < 0,05). A ingestão da torra média também aumentou a concentração da lipoproteína de alta densidade (HDL) em 7 por cento (p = 0,003). Houve aumento nos índices de Castelli após o consumo da torra média-clara (5 por cento no índice I; p = 0,01 e de 6 por cento no índice II; p = 0,03). O TAS dos indivíduos aumentou 21 por cento e 26 por cento , respectivamente, após consumo da torra média-clara e média (p < 0,001). Os indivíduos também tiveram aumento de 13 por cento e 13 por cento na atividade da CAT, 52 por cento e 75 por cento na SOD e 62 por cento e 49 por cento na GPx após a ingestão da torra média-clara e torra média (p < 0,001), respectivamente. Ambas as torras aumentaram as concentrações da molécula de adesão celular vascular-1 solúvel (sVCAM-1), sendo 18 por cento para a torra média-clara e 14 por cento para a torra média) (p < 0,05). A concentração de fibrinogênio plasmático aumentou 8 por cento após ingestão da torra média (p = 0,01) e a selectina-E solúvel (sE-selectina) aumentou 12 por cento após a torra média-clara (p = 0,02). Embora o consumo de café tenha elevado os níveis de colesterol total e LDL, não se relacionou à alteração de homocisteína total, lipoproteína (a) e biomarcadores de diabetes e de peroxidação lipídica. Conclusão: O consumo moderado de café filtrado apresentou alguns efeitos maléficos sobre o perfil de risco cardiovascular em indivíduos saudáveis, independente de sua atividade antioxidante / Introduction: Coffee is rich in bioactive substances and it is among the major contributors to the total antioxidant ingestion in several countries. The roasting degree of coffee is important for its antioxidant activity. Studies indicate that the moderate consumption of filtered coffee is involved in the prevention of chronic diseases, which are usually associated and constitute serious problems of public health. However, literature does not present consensus about the beneficial effects of coffee in the prevention of these diseases. Objectives: To compare the antioxidant activity of the two coffee roasts (medium light and medium roast) and their effects on biomarkers of the cardiovascular risk in healthy volunteers. Methods: The antioxidant characterization of the coffee beverages was performed by the total phenolic content analysis, phenolic profile, caffeine, melanoidins and total antioxidant capacity - TAC (DPPH radical scavenging capacity and Oxygen radical absorbance capacity - ORAC assays). After 1-week washout, twenty healthy volunteers (20 to 65 years old) consumed medium light roast or medium roast paperfiltered coffee for 4 weeks and then switched to the other roast for an additional 4 weeks in a randomized crossover trial that lasted 9 weeks. Plasma lipids, lipoprotein (a), total homocysteine, serum glycemic biomarkers, and twenty-four hours blood pressure were measured at baseline and after each intervention. Levels of total antioxidant status (TAS) and ORAC were evaluated in plasma, and antioxidant enzymes activities (superoxide dismutase - SOD, glutathione peroxidase - GPx and catalase - CAT) in erythrocytes. Lipid peroxidation inhibition capacity was determined in plasma by oxidized LDL and 8-isoprostane assays. Endothelial dysfunction-related inflammation biomarkers were measured in plasma by immunoassays. Results: Twenty healthy volunteers (49.5 + 8.9 years) were evaluated. Medium light roast coffee showed higher chlorogenic acids (334 mg/150 mL; p < 0.001) and less caffeine (231 mg/150 mL; p = 0.003) than medium roasting (210 mg/150 mL and 244 mg/150 mL, respectively). Melanoidins were significant higher in medium roast than medium light roast (p < 0.001). There was an increase in the Castelli indexes after medium light roast consumption (5 per cent in the index I; p = 0.01, and 6 per cent in the index II; p = 0.03). No significant differences were observed for TAC between the medium light roast and medium roast. Both roasts increased plasma total cholesterol and LDL concentrations (10 per cent , and 12 per cent for medium light roast; 12 per cent , and 14 per cent for medium roast, respectively) (p < 0.05). Medium roast also increased HDL concentration by 7 per cent (p = 0.003). Compared with baseline, subjects had an increase of 21 per cent and 26 per cent in TAS, 13 per cent and 13 per cent in CAT, 52 per cent and 75 per cent in SOD, and 62 per cent and 49 per cent in GPx after medium light and medium roast consumption (p < 0.001), respectively. Both roasts increased soluble vascular cell adhesion molecule-1 (sVCAM-1) concentrations (18 per cent for medium light roast and 14 per cent for medium roast) (p < 0.05). Plasma fibrinogen concentration increased 8 per cent after medium roast intake (p = 0.01), and soluble E-selectin (sE-selectin) increased 12 per cent after medium light roast intake (p = 0.02). Although coffee beverages have increased total cholesterol and LDL levels, they were not related to elevation in total homocysteine, lipoprotein (a), and biomarkers of diabetes and lipid peroxidation. Conclusion: Moderate paper-filtered coffee consumption may have some undesirable impact on cardiovascular risk in healthy subjects regardless of its antioxidant content.

O café, rico em substâncias bioativas, está entre os maiores contribuintes para a ingestão de antioxidantes em vários países. O tipo de torra dos grãos influencia em sua atividade antioxidante. Estudos indicam que o consumo moderado de café filtrado está envolvido na redução do risco de doenças crônicas não-transmissíveis, geralmente associadas entre si e que se constituem em graves problemas de saúde pública. Entretanto, a literatura não apresenta consenso sobre a ação benéfica do café na redução do risco destas doenças. Objetivos: Comparar a atividade antioxidante de dois graus de torras de café (torra média-clara e média) e seus efeitos sobre biomarcadores de risco cardiovascular em indivíduos saudáveis. Métodos: A caracterização de antioxidantes nas bebidas foi realizada pelas análises de compostos fenólicos totais, perfil de ácidos fenólicos, cafeína, melanoidinas e capacidade antioxidante total - TAC (sequestro do radical DPPH e capacidade de absorbância do radical oxigênio - ORAC). Após 1 semana de washout, vinte voluntários saudáveis (20 a 65 anos) ingeriram café filtrado preparado com torra média-clara ou torra média por 4 semanas e com o outro tipo de torra por mais 4 semanas em um ensaio clínico randomizado do tipo crossover, o qual durou 9 semanas. Lipídeos plasmáticos, lipoproteína (a), homocisteína total, biomarcadores glicêmicos e pressão arterial de 24 horas foram medidos antes do período de intervenção a após a ingestão de cada torra. A atividade antioxidante foi avaliada no plasma e em eritrócitos respectivamente pela TAC (kit Total Antioxidant Status e ORAC) e da atividade das enzimas antioxidantes (superóxido dismutase - SOD, glutationa peroxidase - GPx e catalase - CAT). A capacidade de inibição da peroxidação lipídica foi avaliada no plasma pelas determinações de lipoproteínas de baixa densidade (LDL) oxidadas e 8-isoprostano. Biomarcadores inflamatórios relacionados à disfunção endotelial foram medidos no plasma por imunoensaios. Resultados: Vinte voluntários saudáveis (49,5 + 8,9 anos) foram avaliados. A torra média-clara apresentou maior teor de ácidos clorogênicos (334 mg/150 mL; p < 0,001) e menor teor de cafeína (231 mg/150 mL; p = 0,003) que a torra média (210 mg/150 mL e 244 mg/150 mL, respectivamente). Os teores de melanoidinas foram significamente maiores na torra média (p < 0,001). A TAC não diferiu entre as bebidas. A ingestão de ambas as torras causou aumento nas concentrações de colesterol total e LDL (10 e 12 por cento para a torra média-clara; 12 por cento e 14 por cento para a torra média) (p < 0,05). A ingestão da torra média também aumentou a concentração da lipoproteína de alta densidade (HDL) em 7 por cento (p = 0,003). Houve aumento nos índices de Castelli após o consumo da torra média-clara (5 por cento no índice I; p = 0,01 e de 6 por cento no índice II; p = 0,03). O TAS dos indivíduos aumentou 21 por cento e 26 por cento , respectivamente, após consumo da torra média-clara e média (p < 0,001). Os indivíduos também tiveram aumento de 13 por cento e 13 por cento na atividade da CAT, 52 por cento e 75 por cento na SOD e 62 por cento e 49 por cento na GPx após a ingestão da torra média-clara e torra média (p < 0,001), respectivamente. Ambas as torras aumentaram as concentrações da molécula de adesão celular vascular-1 solúvel (sVCAM-1), sendo 18 por cento para a torra média-clara e 14 por cento para a torra média) (p < 0,05). A concentração de fibrinogênio plasmático aumentou 8 por cento após ingestão da torra média (p = 0,01) e a selectina-E solúvel (sE-selectina) aumentou 12 por cento após a torra média-clara (p = 0,02). Embora o consumo de café tenha elevado os níveis de colesterol total e LDL, não se relacionou à alteração de homocisteína total, lipoproteína (a) e biomarcadores de diabetes e de peroxidação lipídica. Conclusão: O consumo moderado de café filtrado apresentou alguns efeitos maléficos sobre o perfil de risco cardiovascular em indivíduos saudáveis, independente de sua atividade antioxidante / Introduction: Coffee is rich in bioactive substances and it is among the major contributors to the total antioxidant ingestion in several countries. The roasting degree of coffee is important for its antioxidant activity. Studies indicate that the moderate consumption of filtered coffee is involved in the prevention of chronic diseases, which are usually associated and constitute serious problems of public health. However, literature does not present consensus about the beneficial effects of coffee in the prevention of these diseases. Objectives: To compare the antioxidant activity of the two coffee roasts (medium light and medium roast) and their effects on biomarkers of the cardiovascular risk in healthy volunteers. Methods: The antioxidant characterization of the coffee beverages was performed by the total phenolic content analysis, phenolic profile, caffeine, melanoidins and total antioxidant capacity - TAC (DPPH radical scavenging capacity and Oxygen radical absorbance capacity - ORAC assays). After 1-week washout, twenty healthy volunteers (20 to 65 years old) consumed medium light roast or medium roast paperfiltered coffee for 4 weeks and then switched to the other roast for an additional 4 weeks in a randomized crossover trial that lasted 9 weeks. Plasma lipids, lipoprotein (a), total homocysteine, serum glycemic biomarkers, and twenty-four hours blood pressure were measured at baseline and after each intervention. Levels of total antioxidant status (TAS) and ORAC were evaluated in plasma, and antioxidant enzymes activities (superoxide dismutase - SOD, glutathione peroxidase - GPx and catalase - CAT) in erythrocytes. Lipid peroxidation inhibition capacity was determined in plasma by oxidized LDL and 8-isoprostane assays. Endothelial dysfunction-related inflammation biomarkers were measured in plasma by immunoassays. Results: Twenty healthy volunteers (49.5 + 8.9 years) were evaluated. Medium light roast coffee showed higher chlorogenic acids (334 mg/150 mL; p < 0.001) and less caffeine (231 mg/150 mL; p = 0.003) than medium roasting (210 mg/150 mL and 244 mg/150 mL, respectively). Melanoidins were significant higher in medium roast than medium light roast (p < 0.001). There was an increase in the Castelli indexes after medium light roast consumption (5 per cent in the index I; p = 0.01, and 6 per cent in the index II; p = 0.03). No significant differences were observed for TAC between the medium light roast and medium roast. Both roasts increased plasma total cholesterol and LDL concentrations (10 per cent , and 12 per cent for medium light roast; 12 per cent , and 14 per cent for medium roast, respectively) (p < 0.05). Medium roast also increased HDL concentration by 7 per cent (p = 0.003). Compared with baseline, subjects had an increase of 21 per cent and 26 per cent in TAS, 13 per cent and 13 per cent in CAT, 52 per cent and 75 per cent in SOD, and 62 per cent and 49 per cent in GPx after medium light and medium roast consumption (p < 0.001), respectively. Both roasts increased soluble vascular cell adhesion molecule-1 (sVCAM-1) concentrations (18 per cent for medium light roast and 14 per cent for medium roast) (p < 0.05). Plasma fibrinogen concentration increased 8 per cent after medium roast intake (p = 0.01), and soluble E-selectin (sE-selectin) increased 12 per cent after medium light roast intake (p = 0.02). Although coffee beverages have increased total cholesterol and LDL levels, they were not related to elevation in total homocysteine, lipoprotein (a), and biomarkers of diabetes and lipid peroxidation. Conclusion: Moderate paper-filtered coffee consumption may have some undesirable impact on cardiovascular risk in healthy subjects regardless of its antioxidant content.

Cette thèse est consacrée à l’étude du café « Bourbon Pointu », Coffea arabica var. laurina, né d'une mutation spontanée de la variété Bourbon à l’île de La Réunion. Les teneurs moyennes des composés majoritaires non volatils du café vert sont (pourcentage de matière sèche) les suivantes : saccharose (7,1), trigonelline (1,3), caféine (0,75), acide caféoyl-5- quinique (4,7), acide palmitique (5,0), acide linoléique (6,5). L’analyse sensorielle a permis de mettre au point un profil de torréfaction original de courte durée. Parmi les 145 composés volatils extraits des poudres de café torréfié par SPME, cinq sont majoritaires (teneurs moyennes en ppm): acide acétique (34), 2-furaneméthanol (117), 5-méthyl-2-furfural (166), furfural (144), 2-méthylpyrazine (47). Ces cinq composés volatils extraits par SPE se retrouvent dans le café en tasse : acide acétique (23), 2-furaneméthanol (405), 5-méthyl-2-furfural (36), furfural (85), 2-méthylpyrazine (73) avec en plus, la -butyrolactone (97). Le ratio entre la concentration de la molécule et son seuil de perception définit l’Odor Activity Value (OAV). La conversion des concentrations des composés volatils en unités OAV a permis de dégager neuf molécules ayant un impact olfactif important : 2-furfurylthiol (café torréfié), 2-méthylpropanal (chocolat), dodécanal (agrume), 2-éthylhexan-1-ol (agrume), -pinène (boisé, agrume), furfural (boisé, caramel), 2-hydroxy-3-méthylcyclopent-2-én-1-one (érable), hex-2-énal (pomme verte), 2-méthylbut-2-énal (fruité, vert). La détermination de ces OAV a permis de différencier les trois catégories commerciales au niveau olfactif avec la prédominance d’aldéhydes pour les « Grand cru », le phénylacétaldéhyde pour les « Sublime », et les pyrazines pour les « Authentique ». Les conditions géoclimatiques et les transformations post-récoltes influencent de façon non négligeable la composition chimique du café vert et donc les arômes générés lors de la torréfaction et en conséquence, la répartition des cafés dans les trois catégories. L’ensemble de ces résultats fait du «Bourbon Pointu», un café haut de gamme classé parmi les «cafés gourmets». / This thesis is devoted to the study of “Bourbon Pointu” coffee, Coffea arabica var. laurina, born from a spontaneous mutation of the Bourbon variety in Reunion Island. The mean contents of non-volatile main compounds of green coffee are (percentage of dry matter): sucrose (7.1), trigonellin (1.3), caffeine (0.75), cafeoyl-5-quinic acid (1.7), palmitic acid (5.0), linoleic acid (6.5). Sensory analysis allowed to develop an original short time roasting profile. Among the 145 volatiles compounds extracted from roasted coffee powders by SPME, the mean contents of the five major components (ppm) are: acetic acid (34), 2-furanmethanol (117), 5-methyl-2-furfural (166), furfural (144), 2 methylpyrazine (47). These five volatile compounds extracted by SPE are found in the brew coffee: acetic acid (23), 2-furanmethanol (405), 5-methyl-2-furfural (36), furfural (85), 2- methylpyrazine (73) plus -butyrolactone (97). The ratio of molecule content to its perception threshold defines the “Odor Activity Value” (OAV). The conversion of the contents of volatile compounds in units OAV emphasized nine molecules with an important olfactory impact: 2-furfurylthiol (roasted coffee), 2-methylpropanal (chocolate), dodecanal (citrus), 2-ethylhexan-1-ol (citrus),-pinene (woody, citrus), furfural (woody, caramel), 2-hydroxy-3-methylcyclopent-2-en-1-one (maple), hex-2-enal (green apple), 2-methylbut-2- enal (fruity, green). The determination of these OAV differentiated the three commercial categories related to their typical odor due to the predominance of aldehydes for the "Grand cru", phenylacetaldehyde for "Sublime" and pyrazines for "Authentic". Geoclimatic conditions and post-harvest processing have a significant influence on the green coffee composition, on the flavors generated during roasting and thus, on the coffee distribution in the three categories. All these results define the "Bourbon Pointu" as a premium coffee and classify it as a "specialty coffee."

Tesis por compendio / [ES] El proceso de emulsificación de aceites ricos en compuestos bioactivos permite su mejor aplicación y conservación durante el tiempo de almacenamiento. Entre los diversos mecanismos de emulsificación, se destaca el método de Pickering ya que utiliza nanopartículas sólidas naturales en sustitución de los tensioactivos artificiales. Debido a sus propiedades antioxidantes, no toxicidad y disponibilidad, en este trabajo se estudiaron distintas modificaciones del quitosano para su potencial aplicación como partícula de Pickering. Las modificaciones estudiadas fueron la autoagregación, también denominada desprotonación, y el entrecruzamiento con tripolifosfato de sodio. Se evaluó el comportamiento de estas partículas emulsionando aceite de café tostado, un subproducto de la industria de café con un alto contenido de compuestos bioactivos y compuestos volátiles de interés. Posteriormente, se analizaron las propiedades físico-químicas y la estabilidad de las microcápsulas producidas tras el secado de las emulsiones mediante técnicas de secado por atomización y liofilización. Todas las emulsiones se caracterizaron por tener un comportamiento reológico pseudoplástico, sufriendo desintegración a lo largo del proceso de digestión. Las emulsiones formuladas con nanopartículas de quitosano desprotonadas y menor concentración de aceite mostraron una mejor estabilización y, en consecuencia, una mayor bioaccesibilidad de los compuestos fenólicos totales. Las diferentes nanopartículas de quitosano se caracterizaron estudiando su carga superficial, distribución del tamaño de partícula, microestructura y afinidad agua/aceite. A medida que se aumentó la concentración de estas partículas, se afectó positivamente la viscosidad de las emulsiones debido a la formación de una red tridimensional en la fase continua. Las nanopartículas obtenidas al entrecruzar quitosano con tripolifosfato de sodio fueron más pequeñas, dando como resultado emulsiones con gotas más pequeñas. Las emulsiones de Pickering que contenían un 10% de aceite de café tostado se secaron por atomización y se liofilizaron utilizando las diferentes nanopartículas de quitosano estudiadas y maltodextrina como agentes portadores. Las microcápsulas obtenidas tuvieron la humedad, actividad del agua y solubilidad adecuada para su manipulación y almacenamiento. La presencia de nanopartículas de quitosano permitió una mayor retención de aceite en las microcápsulas y mayor eficiencia de encapsulación. Los compuestos bioactivos y las propiedades antioxidantes se conservaron mejor durante la liofilización. Por otro lado, las microcápsulas obtenidas por atomización presentaron mayor protección de estos compuestos durante la digestión. Las microcápsulas formuladas con nanopartículas desprotonadas fueron sometidas a almacenamiento durante 30 días a 25 ºC. Durante el almacenamiento, se evaluó la protección contra la oxidación de lípidos y la liberación de volátiles. Las isotermas de sorción de agua de estas muestras se determinaron también previamente en las condiciones de almacenamiento. Ambas muestras presentaron isotermas del tipo II, lo que permitió un buen ajuste del modelo de GAB a los datos experimentales. La determinación del índice de peróxido y del contenido de dienos conjugados dio lugar a valores adecuados durante el almacenamiento, aunque las muestras liofilizadas presentaron una ligera tendencia a la oxidación debido a la mayor cantidad de aceite superficial. Aunque hubo ligeras diferencias entre las muestras secas, todas mostraron menos pérdida de aromas totales (~28%) en comparación con el aceite no encapsulado (~51%) al final del almacenamiento. Así, se concluyó que las nanopartículas de quitosano estudiadas fueron eficientes para encapsular el aceite de café tostado y preservar sus características frente a la acción de agentes externos. / [CA] El procés d'emulsificació d'olis rics en compostos bioactius permet la seua millor aplicació i conservació durant el temps d'emmagatzematge. Entre els diversos mecanismes d'emulsificació, destaca el mètode de Pickering, ja que utilitza nanopartícules sòlides naturals en substitució als tensioactius artificials. A causa de la seua propietat antioxidant, de la no toxicitat i de la disponibilitat, aquest treball va buscar analitzar el quitosà i les seues modificacions com potencials partícules de Pickering. Les modificacions estudiades van ser la autoagregació, també anomenada desprotonació, i l'entrecreuament amb tripolifosfat de sodi. Es va avaluar el comportament d'aquestes partícules emulsionant oli de cafè torrat, un subproducte de la indústria del cafè amb un alt contingut de compostos bioactius i volàtils d'interès. Posteriorment, es van analitzar les propietats fisicoquímiques i l'estabilitat de les microcàpsules produïdes després de l'assecat de les emulsions mitjançant tècniques d'assecatge per atomització i liofilització. Totes les emulsions tenien un comportament reològic pseudoplàstic, sofrint desintegració al llarg del procés de digestió. Les emulsions formulades amb nanopartícules de quitosà desprotonades i menor concentració d'oli van mostrar una millor estabilització i, en conseqüència, una major bioaccesibilitat als compostos fenòlics totals. Les diferents nanopartícules de quitosà es van caracteritzar estudiant la seua càrrega superficial, distribució del tamany de partícula, microestructura i afinitat aigua/oli. A mesura que es va augmentar la concentració d'aquestes partícules, es va afectar positivament la viscositat de les emulsions a causa de la formació d'una xarxa tridimensional en la fase contínua. Les nanopartícules obtingudes a l'entrecreuar quitosà amb tripolifosfat de sodi van ser més menudes, donant com a resultat emulsions amb gotes més menudes també. Les emulsions de Pickering que contenien un 10% d'oli de cafè torrat es van assecar per atomització i es liofilitzaren utilitzant les diferents nanopartícules de quitosà estudiades i maltodextrina com a agents portadors. Les microcàpsules obtingudes van obtenir una humitat, activitat de l'aigua i solubilitat adequada per a la seua manipulació i emmagatzematge. La presència de nanopartícules de quitosà va permetre major retenció d'oli en les microcàpsules i major eficiència d'encapsulació. Els compostos bioactius i les propietats antioxidants es van conservar millor durant la liofilització. D'altra banda, les microcàpsules obtingudes per atomització presentaren major protecció d'aquests compostos durant la digestió. Les microcàpsules formulades amb nanopartícules desprotonades també van ser sotmeses a la prova d'emmagatzematge durant 30 dies a 25°C. Durant l'emmagatzematge, es va avaluar la seua protecció contra l'oxidació de lípids i l'alliberament de volàtils. Per això, les isotermes de sorció d'aigua d'aquestes mostres es van determinar prèviament en les condicions d'emmagatzematge. Les dues mostres van presentar isotermes de tipus II, el que va permetre un bon ajust del model de GAB a les dades experimentals. L'índex de peròxids i el contingut de diens conjugats van resultar en valors adequats durant l'emmagatzematge, encara que les mostres liofilitzades van presentar una lleugera tendència a l'oxidació a causa de la major quantitat d'oli superficial. Encara que va haver lleugeres diferències entre les mostres seques, totes van mostrar menys pèrdua d'aromes totals (~28%) en comparació amb l'oli no encapsulat (~51%) a la fi de l'emmagatzematge. Així, es va concloure que les nanopartícules de quitosà estudiades van ser eficients per encapsular l'oli de cafè torrat i preservar les seues característiques enfront de l'acció d'agents externs. / [EN] The emulsification process of bioactive-rich oils makes possible their better application and preservation over the storage time. Among the many emulsification mechanisms, the Pickering method has been highlighted as it uses natural solid nanoparticles in replacement of artificial surfactants. Due to the antioxidant properties, non-toxicity and availability, this work aimed at studying chitosan modifications to produce potential Pickering particles. The studied modifications comprised self-aggregation, also called deprotonation, and crosslinking with sodium tripolyphosphate. The performance of these particles was evaluated in the emulsification of roasted coffee oil, a by-product of the coffee industry with a high content of bioactive and volatile compounds of interest. Subsequently, the physicochemical properties and stability of the microcapsules produced after drying the emulsions using spray-drying and lyophilization techniques were analyzed. All emulsions were characterized as shear-thinning, being them destabilized over the digestion process. Emulsions formulated with deprotonated chitosan nanoparticles and lower oil concentrations showed better stabilization and, consequently, greater bioaccessibility of total phenolic compounds. The different chitosan nanoparticles were characterized regarding surface charge, particle size distribution, microstructure and oil/water affinity. Deprotonated chitosan nanoparticles had a larger particle size, which resulted in emulsions with larger oil droplets. As the concentration of these particles increased, the viscosity of the emulsions was positively affected by the formation of a three-dimensional network in the continuous phase. The nanoparticles obtained by crosslinking with sodium tripolyphosphate were smaller, resulting in emulsions with smaller droplets. The viscosity of these emulsions was lower and little affected by the concentration of particles. Pickering emulsions containing 10% roasted coffee oil were spray-dried and freeze-dried, using the different studied chitosan nanoparticles and maltodextrin as carrier agents. The resulting microcapsules showed adequate moisture content, water activity and solubility for subsequent handling and storage. The presence of chitosan nanoparticles resulted in greater oil retention in the microcapsules and higher encapsulation efficiency. Microcapsules obtained by spray-drying had a more regular spherical shape, while the microparticles obtained by freeze-drying were larger with irregular morphology. Bioactive compounds and antioxidant properties were more preserved during freeze-drying. On the other hand, spray drying allowed greater protection of these compounds during the in vitro digestion. The spray- and freeze-dried microcapsules formulated with deprotonated nanoparticles were subjected to the storage test for 30 days at 25 ºC. During storage, their protection against lipid oxidation and volatile release were evaluated. The water sorption isotherms of these samples were previously determined under the storage conditions. Both samples presented type II isotherms, which resulted in a good fitting accuracy of the GAB model to the experimental data. The peroxide index and the conjugated dienes content resulted in adequate values during storage, although the freeze-dried samples showed a slightly higher tendency to oxidation due to the higher amount of surface oil. Although slight differences were observed between the dried samples, both of them showed less loss of total volatile compounds (~28%) when compared to the non-encapsulated oil (~51%) at the end of storage. Thus, it was concluded that the studied chitosan nanoparticles were efficient to encapsulate roasted coffee oil and to preserve its characteristics against the action of external agents. / The authors would like to thank the project RTI-2018-099738-B-C22 from the ‘Ministerio de Ciencia, Innovación y Universidades’, the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES) - (Finance Code 001; Grant number 88887.468140/2019-00) and the São Paulo Research Foundation (FAPESP – Grant number 2016/22727-8) for the financial support. / Franco Ribeiro, E. (2021). Microencapsulation of roasted coffee oil from chitosan nanoparticles-stabilized Pickering emulsions [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/163234 / Compendio

Conselho Nacional de Desenvolvimento Científico e Tecnológico / Agriculture is one of the main economic activities of the Brazil, possessing the culture of coffee as their main primary commodity. In order to achieve high levels of production and prevent the loss of crops, farm inputs such as pesticides are used in large quantities. This type of practice and the increased public concern consumer are motivating scientific research about these topics, with the aim of ensuring food safety of products supplied for consumption. However, the literature does not report methodologies for the determination of pesticide residues in roasted coffee, with the methods of extraction and analysis proposed, which according to bibliographic data present residues of these substances even after roasting. In this context, the present study sought to develop an analytical methodology to determine carbofuran, cypermethrin, chlorpyrifos, clothianidin, disulfoton, endosulfan, spirodiclofen, haloxyfop, imidacloprid, tebuconazole, triadimenol and triadimefom in roasted coffee using solid liquid extraction method by sonication with clean-up step through liquid liquid extraction (LLE) and instrumental analysis for ultra performance liquid chromatography tandem mass spectrometry detector (LC-MS/MS). For both, the optimization of chromatographic conditions and assessing the best method of clean-up was carried out. With that, the conditions for better quantitative response was obtained with 0.5 g of roasted coffee, 5 mL extraction solvent by sonication, 1 mL of water and 1 mL of dichloromethane in the clean-up by LLE. During the validation procedure in LC-MS/MS positive matrix effect was observed for all analytes except of cypermethrin in which there was suppression of the signal by array, causing, prepared in the curve-based extract, recoveries between 74.2%-102.4% standard relative deviation (SRD) between 0.6% and 10.2%, in levels concentration assessed in the process, Furthermore, the linearity obtained was in the range of 0.9979 to 0.9998 for pesticides , carbofuran, disulfoton, imidacloprid, clothianidin tebuconazole, triadimenol, triadimefom and ensuring the efficiency of the methodology. / A agricultura é uma das principais atividades econômicas do Brasil, possuindo a cultura do café como sua principal commodity primária. A fim de atingir altos níveis de produção e evitar a perda das safras, insumos agrícolas como os agrotóxicos são utilizados em grandes quantidades. Este tipo de prática e o aumento da preocupação do público consumidor estão motivando investigações científicas a respeito destes tópicos, com o intuito de garantir a segurança alimentar dos produtos fornecidos para o consumo. No entanto, a literatura não relata metodologias para a determinação de resíduos de agrotóxicos no café torrado, com os métodos de extração e análise propostos, que segundo dados bibliográficos apresentam resíduos destas substâncias mesmo após a torrefação. Neste contexto, o presente trabalho buscou desenvolver uma metodologia analítica para determinar carbofurano, cipermetrina, clorpirifós, clotianidina, dissulfotom, endosulfan, espirodiclofeno, haloxifope, imidacloprido, tebuconazol, triadimefom e triadimenol em café torrado empregando método de extração sólido líquido por sonicação com etapa de clean-up por meio de extração líquido líquido (LLE) e análise instrumental por cromatografia líquida de ultra eficiência acoplada a detector por espectrometria de massas (LC-MS/MS). Para tanto, a otimização das condições cromatográficas e a avaliação do melhor método de clean-up foram realizadas. Com isso, as condições de melhor resposta quantitativa foi obtida com 0,5 g de café torrado, 5 mL de solvente de extração por sonicação, 1 mL de água e 1 mL de diclorometano na etapa de clean-up por LLE. Durante o procedimento de validação no LC-MS/MS foi observado efeito matriz positivo para todos os analitos com exceção da cipermetrina na qual houve supressão do sinal pela matriz, ocasionando, com base na curva preparada no extrato, recuperações entre 74,2% - 102,4%, com desvio padrão relativo entre 0,6% e 10,2%, nos níveis de concentração avaliados no processo, além disso, a linearidade obtida foi na faixa de 0,9979 a 0,9998 para os agrotóxicos, carbofurano, clotianidina dissulfotom, imidacloprido, tebuconazol, triadimefom e triadimenol, garantindo a eficiência da metodologia.