La rose est l'une des plantes ornementales les plus populaires, dont les composés volatils sont non seulement impliqués dans les interactions des fleurs avec l’environnement au sens large, mais aussi largement utilisés dans l’industrie des arômes et parfums. Le chapitre 1 décrit l'histoire de la culture de la rose, les usages de son parfum, les connaissances actuelles sur la biosynthèse des composés de ce parfum, ainsi que les voies de biosynthèse des composés volatils qui ont été récemment élucidées chez différentes plantes. Les chapitres expérimentaux 2 et 3 analysent les fonctions de deux gènes exprimés dans les pétales de rose. Ils codent pour des protéines Nudix hydrolase 1 (NUDX1). Le gène NUDX1-1 (nommé RhNUDX1 dans la publication) a été découvert en comparant les transcriptomes de deux cultivars de rose, Rosa x hybrida cv. 'Papa Meilland' (PM) très parfumé et R. x hybrida cv. 'Rouge Meilland' (RM), dépourvu de parfum. Le gène RhNUDX1-1 n'est exprimé que chez PM et son expression est corrélée avec la production de monoterpènes dans les pétales, en particulier de géraniol. Lors de l'étude d'une descendance issue du croisement de R. chinensis cv. ‘Old Blush’ (OB) et de R. x wichurana (Rw), le gène orthologue RcNUDX1-1a, présentant la même fonction, a été caractérisé chez OB. Un gène paralogue, RwNUDX1-2, a été découvert chez Rw et il a été démontré que son expression présentait une corrélation avec la production sesquiterpènes, en particulier de E,E-farnesol. Une série d'analyses in vitro et in vivo ainsi qu'une analyse de corrélation ont permis de vérifier la fonction de RhNUDX1-1, qui hydrolyse le géranyl diphosphate (GPP) en géranyl monophosphate (GP). Une phosphatase non identifiée pourrait catalyser la transformation du GP en géraniol. Des expériences de fusion avec la Green Fluorescent Protein (GFP), suivies de transformation transitoire de feuilles de tabac, ont révélé que RhNUDX1-1 était localisée dans le cytoplasme. Les mêmes approches (analyses QTL, essais enzymatiques et expression transitoire) ont également été appliquées à RwNUDX1-2, démontrant sa fonction dans la production de E,E-farnesol. La cartographie de RwNUDX1-2 et la localisation subcellulaire de la protéine sont encore à l'étude. De plus, la cristallographie des protéines et la modélisation ont été employées pour étudier le mécanisme de l'interaction NUDX1-substrat et les acides aminés potentiellement importants pour la reconnaissance du substrat. Collectivement, ces données révèlent une voie alternative pour la biosynthèse des terpènes, en particulier le géraniol et E,E-farnesol, via l'hydrolyse des prényl diphosphates par les enzymes NUDX1. Nos résultats montrent que la production de composés volatils dans les pétales est fortement corrélée avec l’expression des gènes des voies de biosynthèse. Par conséquent, la régulation transcriptionnelle de RcNUDX1-1a et RwNUDX1-2 joue probablement un rôle important dans la production de parfum. Les promoteurs de RcNUDX1-1a, RcNUDX1-1b, et RwNUDX1-2 et deux facteurs de transcription (FT), RcbHLH79 (OB TF) et RwbHLH79 (Rw TF) ont ainsi été isolés et testés (Chapitre 4). Les FT candidats ont été choisis lors d’une analyse RNA-Seq (Chapitre 5). En utilisant des tests d'expression transitoire avec le gène rapporteur GUS (β-glucuronidase) dans les pétales de rose, il a été montré que les trois promoteurs pouvaient entraîner l'expression de GUS. Les deux FT ont ensuite été introduits dans des feuilles de tabac avec les promoteurs testés, pour voir s'ils étaient capables d'activer ces promoteurs. Aucune transactivation significative n'a été détectée, même si Rw TF semblait pouvoir activer une construction témoin (promoteur du gène de la tomate TPS5. Les transcriptomes de quatre cultivars de rose, dont deux produisent du géraniol mais pas de E,E-farnesol et deux autres produisent du E,E-farnesol mais pas de géraniol, ont été analysés (Chapitre 5) et ont abouti à une liste de FT putatifs pour une étude plus approfondie / Roses are one of the most popular ornamental plants, whose volatiles are not only involved in environmental interactions but also widely used for industries. Chapter 1 describes the cultivation history of roses, usages of rose fragrance, knowledge on the biosynthesis of rose scent compounds, as well as non-canonical biosynthesis pathways of other plant volatiles. Experimental chapters (Chapter 2 and 3) analyse the functions of two genes expressed in rose petals, both encoding Nudix hydrolase 1 (NUDX1) protein. NUDX1-1 gene (named RhNUDX1) was first discovered by comparing the transcriptomes of two rose cultivars, the scented Rosa x hybrida cv. ‘Papa Meilland’ (PM) and the unscented R. x hybrida cv. ‘Rouge Meilland’ (RM). RhNUDX1-1 was only expressed in scented PM and its expression exhibited a positive correlation with the monoterpenoid production in petals, especially geraniol. When studying a rose progeny of R. chinensis cv. ‘Old Blush’ (OB) and R. x wichurana (Rw), an orthologous gene RcNUDX1-1a was found in OB, whose expression also had positive correlation with geraniol emission. A paralogous gene in Rw, RwNUDX1-2, was discovered and it was shown that its expression displayed a correlation with the sesquiterpenoid production, especially E,E-farnesol. A series of in vitro and in vivo assays as well as correlation analyses verified the function of RhNUDX1-1, which hydrolysed geranyl diphosphate (GPP) to geranyl monophosphate (GP). The transformation of GP into geraniol is supposed to be processed by an, as yet, unidentified phosphatase. The prediction of the localisation together with green fluorescent protein (GFP) fusion experiments revealed that RhNUDX1-1 was located in the cytosol. A series of approaches (QTL analyses, enzymatic assays and transient expression studies) were also applied to RwNUDX1-2, demonstrating its function in the production of E,E-farnesol. Mapping of RwNUDX1-2 and subcellular localization of the protein are still under investigation. Furthermore, protein crystallography and protein modelling illustrated the NUDX1-substrate interaction and proposed several residues that may be important for substrate recognition, although further experimental and computational data are required to gain more insight into the enzymatic mechanism. Collectively, these data revealed an alternative pathway for the biosynthesis of terpenoids, especially geraniol and E,E-farnesol, in rose, via the hydrolysis of prenyl diphosphates by NUDX1 enzymes. Transcriptional regulation of RcNUDX1-1a or RwNUDX1-2 probably plays an important role in the scent production by rose petals. Therefore, three promoters, pOB1a (promoter of RcNUDX1-1a), pOB1b (promoter of RcNUDX1-1b, not expressed in rose petals), pRw (promoter of RwNUDX1-2) were cloned and tested (Chapter 4). In addition, two transcription factors (TFs), RcbHLH79 (OB TF) and RwbHLH79 (Rw TF) candidates were chosen via RNA-Seq analysis as their expression correlated with expression of RcNUDX1-1a or RwNUDX1-2, respectively (Chapter 5). Using transient expression assays with a reporter gene, β-glucuronidase (GUS) in rose petals, it was shown that all three promoters could drive the expression of GUS, suggesting that all of them are active. However, quantification of promoter activities is still needed. OB TF and Rw TF were introduced into Nicotiana benthamiana leaves together with the promoters driving GUS , to determine if they were able to activate these promoters. However, no significant transactivation was detected in any promoter-TF combination. The expression of the TF in the progeny was also analysed but, due to the similarity of the sequences of family members, no conclusive data were obtained. Transcriptomes of the petals four roses, two of which produce geraniol but not E,E-farnesol and two that produce E,E-farnesol but not geraniol, were analysed (Chapter 5) and this resulted in a list of putative scent related genes and transcription factors for further study

As moscas-das-frutas (Diptera: Tephritidae) são as principais pragas da fruticultura no mundo, pelos danos que ocasionam diretamente e indiretamente e pela facilidade de adaptação. O manejo das moscas-das-frutas através do monitoramento da população com diferentes substâncias atrativas mais eficientes é de suma importância. Assim, o objetivo com este trabalho foi avaliar a eficiência da inclusão do acetato de amônia em diferentes concentrações nas soluções compostas de proteína hidrolisada de milho e açúcares. O experimento 1 foi realizado em pomar de goiaba durante 7 semanas e avaliadas 6 soluções atrativas: Isca Samaritá® Tradicional (5%), Isca Samaritá® Tradicional + 3% de acetato de amônia (5% + 3%), Isca Samaritá® Tradicional + 5% de acetato de amônia (5% + 5%), Isca Samaritá® Tradicional + 7% de acetato de amônia (5% + 7%), Melaço (7%) e pastilhas de torula (1%). O delineamento experimental utilizado foi o casualizado em blocos com 6 tratamentos e 4 repetições. As armadilhas utilizadas foram do tipo McPhail. As trocas eram feitas a cada 7 dias. Não houve diferença para o total de moscas-das-frutas capturadas pelas soluções de torula e Isca Samaritá® Tradicional + 3% e 7% de acetato de amônia, sendo as mais atrativas. Também não houve diferença na captura de fêmeas de moscas-das-frutas para os atrativos torula e Isca Samaritá® Tradicional + 3% de acetato de amônia. Para insetos úteis e inimigos naturais as soluções com melaço e torula foram as menos seletivas. Com a adição de acetato de amônia foi possível verificar um aumento considerável na atratividade em relação ao produto comercial, principalmente no primeiro dia após a instalação no campo. A solução Isca Samaritá® Tradicional + 3% de Acetato de Amônia foi selecionada como o atrativo com melhor custo/benefício com adição de acetato de amônia testado e avaliado a eficiência da atratividade no experimento 2 comparando com Isca Samaritá® Tradicional (5%), Melaço (7%) e pastilhas de torula (1%). Utilizou-se a metodologia de marcação-liberação-recaptura de A. fraterculus estéreis. A dispersão foi monitorada a partir de um ponto central de liberação nas direções cardeais e colaterais utilizando armadilhas McPhail. A direção de dispersão através da análise circular exposta pelo Rose Diagram, identificou que as maiores frequências de recaptura foram nas posições onde se encontravam a torula e a Isca Samaritá® Tradicional + 3% de acetato de amônia. / The fruit flies (Diptera: Tephritidae) are the main pests of fruit crops in the world, due to the direct and indirect damage that its causes and easy of adaptation. The management of the fruit flies through population monitoring with different attractive substances more efficient is very important. The objective with this study was to evaluate the efficiency of the inclusion of ammonium acetate at different concentrations in the solutions composed of corn hydrolysed protein and sugars. The experiment 1 was conducted in guava orchard during seven weeks and evaluated 6 attractives solutions: Isca Samaritá® Traditional (5%), Isca Samaritá® Traditional + 3% ammonium acetate (5% + 3%), Isca Samaritá® Traditional + 5% ammonium acetate (5% + 5%), Isca Samaritá® Traditional + 7% of ammonium acetate (5% + 7%), molasses (7%) and torula pellets (1%). The experimental design was a randomized block with 6 treatments and 4 repetitions. The traps used were of the McPhail type. Exchanges were make every 7 days. There was not difference for the total the fruit flies captured by torula solutions and Isca Samaritá® Traditional + 3% and 7% of ammonium acetate, the most attractive. There was not difference in female fruit flies captured by torula solutions and Isca Samaritá® Traditional + 3% ammonium acetate. For social insects and natural enemy the solutions with molasses and torula were less selective. With the addition of ammonium acetate it was possible to see a considerable increase in attractiveness to the commercial product, especially on the first day after installation in the field. The solution Isca Samaritá® Traditional + 3% Ammonium Acetate was selected as the best cost benefit with the addition of ammonium acetate tested and evaluated the attractiveness of efficiency in experiment 2 compared to Isca Samaritá® Traditional (5%), molasses (7%) and torula pellets (1%). It used the methodology of mark-release-recapture A. fraterculus sterile. The dispersion was monitored from a central point of release and the side cardinal directions, in McPhail traps. The direction of dispersion through the circular analysis exposed by Rose Diagram identified that the greatest frequency of recaptures were in positions where they were the torula and Isca Samaritá® Traditional + 3% ammonium acetate.

Este trabalho tem como objetivo trazer uma visão geral da poética de Rose Ausländer ao mesmo tempo em que apresenta importantes aspectos de sua biografia, como sua formação, as influências sofridas e exercidas, o contexto histórico e geográfico em que viveu, os anos de confinamento no gueto em Czernowitz, o exílio, a vida no pós-guerra e o reflexo de sua experiência de vida em sua obra. Também propõe uma reflexão sobre o pensamento de Theodor Adorno, cujas ponderações sobre Auschwitz estão entre as mais preciosas que a filosofia já propôs sobre o assunto. Assim, após uma apresentação sucinta do conceito de arte adorniano, o trabalho irá refletir a cerca da polêmica da lírica após Auschwitz, da oscilação entre possibilidade e impossibilidade de arte e do risco de banalização da catástrofe em sua representação estética. Estas questões serão esmiuçadas, dialogando com poemas de Rose Ausländer. Em seguida, será feito um esboço do desenvolvimento da forma e estilo na poesia de Rose Ausländer ao longo das três fases de seu processo de criação. Neste momento do trabalho será observado como se dá a variação dos temas no desenvolvimento de sua obra poética e mais especificamente, como a experiência do holocausto é recriada em sua poesia. Por fim será feita uma reflexão sobre em que medida é possível se afirmar que a poética de Rose Ausländer cumpre a função adorniana da obra de arte constituindo-se deste modo, numa representação sociológica autêntica e de crítica à violência. / The paper provides an insight into the poetics of Rose Ausländer. Her work is being placed within the historical context of the time and within the biographical context of the poetess - e.g. her detention in the Ghetto of Czernowitz, her exile and her life after the war. Thus, the consequences of her life experience for her work are being revealed. Subsequently, her work is being discussed on the basis of Theodor Adorno\'s thoughts on Auschwitz. After a short introduction to Adorno\'s concept of art, the paper adresses the controversy about possibility or impossibility of art after Auschwitz and the risk of trivialisation of Auschwitz through esthetical presentation. Finally, the paper traces the development of form and style within Rose Ausländer\'s lyrics through her phases of creative process and shows the variation of subjects. Here, the paper elaborates especially how the experience of the Holocaust is being reflected in her work. The paper ends with a discussion of the question whether the poetics of Rose Ausländer meets Adornos\' criterias of artwork, thus, whether it is an authentic and sociological portrayal which realizes a critique of violence.

Este trabalho tem como objetivo trazer uma visão geral da poética de Rose Ausländer ao mesmo tempo em que apresenta importantes aspectos de sua biografia, como sua formação, as influências sofridas e exercidas, o contexto histórico e geográfico em que viveu, os anos de confinamento no gueto em Czernowitz, o exílio, a vida no pós-guerra e o reflexo de sua experiência de vida em sua obra. Também propõe uma reflexão sobre o pensamento de Theodor Adorno, cujas ponderações sobre Auschwitz estão entre as mais preciosas que a filosofia já propôs sobre o assunto. Assim, após uma apresentação sucinta do conceito de arte adorniano, o trabalho irá refletir a cerca da polêmica da lírica após Auschwitz, da oscilação entre possibilidade e impossibilidade de arte e do risco de banalização da catástrofe em sua representação estética. Estas questões serão esmiuçadas, dialogando com poemas de Rose Ausländer. Em seguida, será feito um esboço do desenvolvimento da forma e estilo na poesia de Rose Ausländer ao longo das três fases de seu processo de criação. Neste momento do trabalho será observado como se dá a variação dos temas no desenvolvimento de sua obra poética e mais especificamente, como a experiência do holocausto é recriada em sua poesia. Por fim será feita uma reflexão sobre em que medida é possível se afirmar que a poética de Rose Ausländer cumpre a função adorniana da obra de arte constituindo-se deste modo, numa representação sociológica autêntica e de crítica à violência. / The paper provides an insight into the poetics of Rose Ausländer. Her work is being placed within the historical context of the time and within the biographical context of the poetess - e.g. her detention in the Ghetto of Czernowitz, her exile and her life after the war. Thus, the consequences of her life experience for her work are being revealed. Subsequently, her work is being discussed on the basis of Theodor Adorno\'s thoughts on Auschwitz. After a short introduction to Adorno\'s concept of art, the paper adresses the controversy about possibility or impossibility of art after Auschwitz and the risk of trivialisation of Auschwitz through esthetical presentation. Finally, the paper traces the development of form and style within Rose Ausländer\'s lyrics through her phases of creative process and shows the variation of subjects. Here, the paper elaborates especially how the experience of the Holocaust is being reflected in her work. The paper ends with a discussion of the question whether the poetics of Rose Ausländer meets Adornos\' criterias of artwork, thus, whether it is an authentic and sociological portrayal which realizes a critique of violence.

As moscas-das-frutas (Diptera: Tephritidae) são as principais pragas da fruticultura no mundo, pelos danos que ocasionam diretamente e indiretamente e pela facilidade de adaptação. O manejo das moscas-das-frutas através do monitoramento da população com diferentes substâncias atrativas mais eficientes é de suma importância. Assim, o objetivo com este trabalho foi avaliar a eficiência da inclusão do acetato de amônia em diferentes concentrações nas soluções compostas de proteína hidrolisada de milho e açúcares. O experimento 1 foi realizado em pomar de goiaba durante 7 semanas e avaliadas 6 soluções atrativas: Isca Samaritá® Tradicional (5%), Isca Samaritá® Tradicional + 3% de acetato de amônia (5% + 3%), Isca Samaritá® Tradicional + 5% de acetato de amônia (5% + 5%), Isca Samaritá® Tradicional + 7% de acetato de amônia (5% + 7%), Melaço (7%) e pastilhas de torula (1%). O delineamento experimental utilizado foi o casualizado em blocos com 6 tratamentos e 4 repetições. As armadilhas utilizadas foram do tipo McPhail. As trocas eram feitas a cada 7 dias. Não houve diferença para o total de moscas-das-frutas capturadas pelas soluções de torula e Isca Samaritá® Tradicional + 3% e 7% de acetato de amônia, sendo as mais atrativas. Também não houve diferença na captura de fêmeas de moscas-das-frutas para os atrativos torula e Isca Samaritá® Tradicional + 3% de acetato de amônia. Para insetos úteis e inimigos naturais as soluções com melaço e torula foram as menos seletivas. Com a adição de acetato de amônia foi possível verificar um aumento considerável na atratividade em relação ao produto comercial, principalmente no primeiro dia após a instalação no campo. A solução Isca Samaritá® Tradicional + 3% de Acetato de Amônia foi selecionada como o atrativo com melhor custo/benefício com adição de acetato de amônia testado e avaliado a eficiência da atratividade no experimento 2 comparando com Isca Samaritá® Tradicional (5%), Melaço (7%) e pastilhas de torula (1%). Utilizou-se a metodologia de marcação-liberação-recaptura de A. fraterculus estéreis. A dispersão foi monitorada a partir de um ponto central de liberação nas direções cardeais e colaterais utilizando armadilhas McPhail. A direção de dispersão através da análise circular exposta pelo Rose Diagram, identificou que as maiores frequências de recaptura foram nas posições onde se encontravam a torula e a Isca Samaritá® Tradicional + 3% de acetato de amônia. / The fruit flies (Diptera: Tephritidae) are the main pests of fruit crops in the world, due to the direct and indirect damage that its causes and easy of adaptation. The management of the fruit flies through population monitoring with different attractive substances more efficient is very important. The objective with this study was to evaluate the efficiency of the inclusion of ammonium acetate at different concentrations in the solutions composed of corn hydrolysed protein and sugars. The experiment 1 was conducted in guava orchard during seven weeks and evaluated 6 attractives solutions: Isca Samaritá® Traditional (5%), Isca Samaritá® Traditional + 3% ammonium acetate (5% + 3%), Isca Samaritá® Traditional + 5% ammonium acetate (5% + 5%), Isca Samaritá® Traditional + 7% of ammonium acetate (5% + 7%), molasses (7%) and torula pellets (1%). The experimental design was a randomized block with 6 treatments and 4 repetitions. The traps used were of the McPhail type. Exchanges were make every 7 days. There was not difference for the total the fruit flies captured by torula solutions and Isca Samaritá® Traditional + 3% and 7% of ammonium acetate, the most attractive. There was not difference in female fruit flies captured by torula solutions and Isca Samaritá® Traditional + 3% ammonium acetate. For social insects and natural enemy the solutions with molasses and torula were less selective. With the addition of ammonium acetate it was possible to see a considerable increase in attractiveness to the commercial product, especially on the first day after installation in the field. The solution Isca Samaritá® Traditional + 3% Ammonium Acetate was selected as the best cost benefit with the addition of ammonium acetate tested and evaluated the attractiveness of efficiency in experiment 2 compared to Isca Samaritá® Traditional (5%), molasses (7%) and torula pellets (1%). It used the methodology of mark-release-recapture A. fraterculus sterile. The dispersion was monitored from a central point of release and the side cardinal directions, in McPhail traps. The direction of dispersion through the circular analysis exposed by Rose Diagram identified that the greatest frequency of recaptures were in positions where they were the torula and Isca Samaritá® Traditional + 3% ammonium acetate.

Les eaux florales sont des matières premières aromatiques issues de la distillation, contenant généralement moins de 1 g/L de composés volatils leur conférant leurs propriétés organoleptiques. Elles sont utilisées principalement en industrie agroalimentaire et cosmétique. Elles sont sujettes à des problèmes d’instabilité microbienne incompatibles avec leurs applications. Ces microorganismes, leurs dynamiques, ainsi que les nutriments disponibles nécessaires à leur croissance restent mal connus. Les eaux florales sont actuellement stabilisées par ajout de conservateurs dont certains sont controversés et visent à être retirés du marché. De plus, leur efficacité dans les eaux florales n’a pas été évaluée.L’objectif de cette thèse est d’apporter une meilleure connaissance de la composition des eaux florales et de ses contaminants afin de proposer une méthode de stabilisation adaptée.La composition en huile essentielle et le microbiote de 22 échantillons d’eau de fleur d’oranger (Citrus aurantium L. ssp. amara L.) et de rose (Rosa damascena Miller et Rosa centifolia L.), provenant de différents producteurs autour du bassin méditerranéen, ont été analysés afin de déterminer les facteurs responsables de leur altération. Bien que les composés volatils soient connus pour leurs propriétés antimicrobiennes, leurs concentrations dans les hydrolats ne sont pas suffisantes pour assurer la stabilité microbiologique. En plus des composés volatils, les hydrolats contiennent des composés non-volatils tels que des sucres, entraînés vers le distillat par effet de primage ou de moussage pendant la distillation, et pouvant être utilisés comme substrat de croissance par les microorganismes. La population microbienne peut atteindre 106 à 107 UFC/mL en quelques jours à température ambiante et jusqu’à 3 mois à 5°C. Des bactéries environnementales, oligotrophes, et acido-tolérantes, appartenant principalement aux genres Pseudomonas sp. et Burkholderia sp. ont été isolées et identifiées. Parmi ces bactéries, B. vietnamiensis et Novosphingobium capsulatum ont été capables de métaboliser des composés volatiles tels que le géraniol ou l’acétate de 2-phényléthyle pour produire la 6-méthyl-5-heptèn-2-one ou le 2-phényléthanol, et modifier ainsi les propriétés organoleptiques des hydrolats. Enfin, la capacité de croissance de bactéries pathogènes et d’altération dans les hydrolats a été évaluée, et différents conservateurs ont été testés sur les souches capables de se multiplier dans les hydrolats.Une distillation aseptique et un conditionnement stérile permettrait d’assurer la stabilité des hydrolats sans ajout de conservateurs. En l’absence de conditions aseptiques, l’ajout de conservateurs est nécessaire pour assurer la stabilité des hydrolats. / Hydrosols are hydrodistillation products mainly used as food flavoring agents or ingredient in cosmetics. They contain less than 1 g/L of dispersed essential oils giving the organoleptic properties. These are subjected to microbial proliferation that can prevent use due to non-compliance to professional microbiological standards. The microorganisms, their growth dynamics, and the available nutrients in hydrosols remain unknown. Hydrosols can contain few preservatives, but there is no data about their efficiency in hydrosols.The aim of this study was to have a better knowledge on hydrosols composition, their microbiota, and spoilage conditions, in order to propose an adapted stabilization method.The composition in volatile compounds and the microbiota of 22 hydrosol samples of Citrus aurantium L. ssp. amara L. (orange blossom), Rosa damascena Miller (rose D.), and Rosa centifolia L. (rose C.) flowers were analyzed to determine factors responsible for decay. Some non-volatile compounds were likely carried over during distillation by a priming and foaming effect, and could be used as nutrients by microorganisms. Concentrations of volatile compounds in hydrosols are not high enough to prevent microbial proliferation, and bacteria concentrations can reach up to 106 CFU/mL in both hydrosols. The isolated microbial population was composed of oligotrophic Gram-negative bacteria, arranged in four major genera: Pseudomonas sp., Burkholderia cepacia complex, and presumably two new genera belonging to Acetobacteraceae and Rhodospirillaceae. Among those bacteria, Burkholderia vietnamiensis and Novosphingobium capsulatum were able to metabolize volatile compounds, such as geraniol to produce 6-methyl-5-hepten-2-one or geranic acid, or 2-phenylethyl acetate to produce 2-phenylethanol. Finally, the growth potential of a range of bacteria isolated from hydrosols and of pathogenic micro-organisms was evaluated, then the anti-microbial activity in nutrient broth and/or in hydrosols of a range of chemical preservatives authorized for food and cosmetic applications was tested.Additional hurdles such as chemical preservatives or aseptic packaging will be necessary to insure microbial stability.

Thesis (MEd)--Stellenbosch University, 1946. / Some digitised pages may appear illegible due to the condition of the original hard copy. / ENGLISH ABSTRACT: no abstract available / AFRIKAANSE OPSOMMING: geen opsomming

The “Rose Herbert Community Center” is the culmination of a project questioning how a building can be restored to its original integrity when its initial function has become extinct. This thesis considers the Broad Street Station in Richmond, Virginia and explores the options and implications of returning the building to a hub of interaction within the community. Concepts such as functionally malleable spaces, the transition from a very public environment to a more private area, and the creation of intentional interaction versus coexistence are explored.

A floricultura no Brasil, que inclui flores e plantas ornamentais, vem gradualmente crescendo como atividade econômica. Considerando a carência de informações sobre as exigências nutricionais de roseiras, nas condições de produção no Brasil, objetivou-se: a) determinar a curva de crescimento pelo acúmulo de matéria seca de plantas cultivadas em solo, em ambiente protegido; b) estudar a técnica de subtração de nutrientes, para o desenvolvimento e a caracterização dos sintomas de deficiência da cultura cultivada em solução nutritiva em casa de vegetação; e c) avaliar as concentrações e os acúmulos de nutrientes, durante os experimentos em solo e em solução nutritiva. Plantas de Rosa sp. cultivares Vegas e Tineke, foram avaliadas aos 60, 88, 116, 144, 172, 200, 228 e 256 dias após o transplantio, em um delineamento inteiramente casualizado, no período de novembro de 2005 a setembro de 2006. Plantas do cultivar Vegas, foram cultivadas, em casa de vegetação, com solução nutritiva completa e solução com omissão de N, P, K, Ca, Mg, S, B, Cu, Fe, Mn e Zn, em delineamento inteiramente casualizado, no período de agosto a novembro de 2007. A produção de material seco da parte aérea foi crescente durante o período analisado para os cultivares Vegas e Tineke. As plantas do cultivar Vegas apresentaram uma demanda crescente dos macronutrientes e um acúmulo máximo de B, Cu, Fe, Mn e Zn entre 166 e 230 dias após o transplantio. O cultivar Tineke apresentou uma demanda crescente por N, P, K, Ca, S, B, Cu, Fe, Mn e Zn. Os sintomas de deficiência nutricional foram caracterizados, de acordo com os sintomas típicos para a maioria das culturas, exceto para o Cu e Mn. A omissão dos nutrientes afetou a produção de material seco das folhas velhas, folhas novas, caule, rosas e raízes. As concentrações dos nutrientes nas folhas velhas das plantas desenvolvidas em solução nutritiva completa foram: 20,79; 2,52; 22,83; 17,19; 3,30 e 1,37 g kg-1, respectivamente, para N, P, K, Ca, Mg e S e, 148,64; 0,63; 151,40; 129,00 e 9,83 mg kg-1 respectivamente para B, Cu, Fe, Mn e Zn. A ordem de importância dos nutrientes, representada pelo acúmulo nas folhas velhas, foi: K > N > Ca > Mg >P > S > Fe > B > Mn > Zn > Cu e nas flores foi: N > K > P > Ca > Mg > S > Fe > Mn > B > Zn > Cu. / Considering the little information on roses mineral nutrition at production conditions in Brazil, this study objectives were: a) to determine plants dry matter accumulation curve, grown in soil under protected conditions; b) to study nutrient absence technique for the development and deficiency symptoms characterization of a culture grown on nutrient solution in greenhouse and c) to evaluate the contents and nutrients accumulations, during the soil and nutrient solution experiments. Rosa sp. cultivar Vegas and Tineke plants were collected and evaluated to the 60, 88, 116, 144, 172, 200, 228 and 256 days after transplanting in greenhouse condition, in a completely randomized design, from November of 2005 to September of 2006. Cultivar Vegasplants grown in greenhouse, with complete nutrient solution and solution with N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn omission, in a completely randomized design, from August to November of 2007. Aerial part dry matter production increased during the analyzed period to cultivar Vegas and Tineke. Vegascultivar plants presented an increasing demand of the macronutrients and a maximum accumulation of B, Cu, Fe, Mn and Zn between 166 and 230 days after the transplanting. Cultivar Tineke presented an increasing demand for N, P, K, Ca, S, B, Cu, Fe, Mn and Zn. The nutrient deficiency symptoms were characterized in accordance with typical symptoms for the majority of cultures, except for the Cu and Mn. The nutrients omission affected dry matter production of old leaves, young leaves, stem, flowers and roots. The nutrient contents in the old leaves of plants grown in complete nutrient solution were: 20,79; 2,52; 22,83; 17,19; 3,30 and 1,37 g kg-1, respectively, for N, P, K, Ca, Mg and S and, 148,64; 0,63; 151,40; 129,00 and 9,83 mg kg-1, respectively, for B, Cu, Fe, Mn and Zn. The nutrients importance order, represented for old leaves accumulation were: K > N > Ca > Mg > P > S > Fe > B > Mn > Zn > Cu and, in flowers were: N > K > P > Ca > Mg > S > Fe > Mn > B > Zn > Cu.

A floricultura no Brasil, que inclui flores e plantas ornamentais, vem gradualmente crescendo como atividade econômica. Considerando a carência de informações sobre as exigências nutricionais de roseiras, nas condições de produção no Brasil, objetivou-se: a) determinar a curva de crescimento pelo acúmulo de matéria seca de plantas cultivadas em solo, em ambiente protegido; b) estudar a técnica de subtração de nutrientes, para o desenvolvimento e a caracterização dos sintomas de deficiência da cultura cultivada em solução nutritiva em casa de vegetação; e c) avaliar as concentrações e os acúmulos de nutrientes, durante os experimentos em solo e em solução nutritiva. Plantas de Rosa sp. cultivares Vegas e Tineke, foram avaliadas aos 60, 88, 116, 144, 172, 200, 228 e 256 dias após o transplantio, em um delineamento inteiramente casualizado, no período de novembro de 2005 a setembro de 2006. Plantas do cultivar Vegas, foram cultivadas, em casa de vegetação, com solução nutritiva completa e solução com omissão de N, P, K, Ca, Mg, S, B, Cu, Fe, Mn e Zn, em delineamento inteiramente casualizado, no período de agosto a novembro de 2007. A produção de material seco da parte aérea foi crescente durante o período analisado para os cultivares Vegas e Tineke. As plantas do cultivar Vegas apresentaram uma demanda crescente dos macronutrientes e um acúmulo máximo de B, Cu, Fe, Mn e Zn entre 166 e 230 dias após o transplantio. O cultivar Tineke apresentou uma demanda crescente por N, P, K, Ca, S, B, Cu, Fe, Mn e Zn. Os sintomas de deficiência nutricional foram caracterizados, de acordo com os sintomas típicos para a maioria das culturas, exceto para o Cu e Mn. A omissão dos nutrientes afetou a produção de material seco das folhas velhas, folhas novas, caule, rosas e raízes. As concentrações dos nutrientes nas folhas velhas das plantas desenvolvidas em solução nutritiva completa foram: 20,79; 2,52; 22,83; 17,19; 3,30 e 1,37 g kg-1, respectivamente, para N, P, K, Ca, Mg e S e, 148,64; 0,63; 151,40; 129,00 e 9,83 mg kg-1 respectivamente para B, Cu, Fe, Mn e Zn. A ordem de importância dos nutrientes, representada pelo acúmulo nas folhas velhas, foi: K > N > Ca > Mg >P > S > Fe > B > Mn > Zn > Cu e nas flores foi: N > K > P > Ca > Mg > S > Fe > Mn > B > Zn > Cu. / Considering the little information on roses mineral nutrition at production conditions in Brazil, this study objectives were: a) to determine plants dry matter accumulation curve, grown in soil under protected conditions; b) to study nutrient absence technique for the development and deficiency symptoms characterization of a culture grown on nutrient solution in greenhouse and c) to evaluate the contents and nutrients accumulations, during the soil and nutrient solution experiments. Rosa sp. cultivar Vegas and Tineke plants were collected and evaluated to the 60, 88, 116, 144, 172, 200, 228 and 256 days after transplanting in greenhouse condition, in a completely randomized design, from November of 2005 to September of 2006. Cultivar Vegasplants grown in greenhouse, with complete nutrient solution and solution with N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn omission, in a completely randomized design, from August to November of 2007. Aerial part dry matter production increased during the analyzed period to cultivar Vegas and Tineke. Vegascultivar plants presented an increasing demand of the macronutrients and a maximum accumulation of B, Cu, Fe, Mn and Zn between 166 and 230 days after the transplanting. Cultivar Tineke presented an increasing demand for N, P, K, Ca, S, B, Cu, Fe, Mn and Zn. The nutrient deficiency symptoms were characterized in accordance with typical symptoms for the majority of cultures, except for the Cu and Mn. The nutrients omission affected dry matter production of old leaves, young leaves, stem, flowers and roots. The nutrient contents in the old leaves of plants grown in complete nutrient solution were: 20,79; 2,52; 22,83; 17,19; 3,30 and 1,37 g kg-1, respectively, for N, P, K, Ca, Mg and S and, 148,64; 0,63; 151,40; 129,00 and 9,83 mg kg-1, respectively, for B, Cu, Fe, Mn and Zn. The nutrients importance order, represented for old leaves accumulation were: K > N > Ca > Mg > P > S > Fe > B > Mn > Zn > Cu and, in flowers were: N > K > P > Ca > Mg > S > Fe > Mn > B > Zn > Cu.