Sulfate- and Fe(III)-reducing, and methanogenic prokaryotes (SRP, FRP, MGP) are key players in metabolic pathways involved in anaerobic biodegradation processes. Understanding the metabolic activity of these microbes in environments can enhance microbe-mediated processes such as oil spill bioremediation and methane biogas production. In this study, anaerobic microbial activities in Deepwater Horizon oil spill-impacted salt marsh sediments, and in methanogenic coal bed production water enrichment cultures amended with trace elements (TE), were elucidated by employing an approach combining methods in molecular biology and geochemistry. In situ metabolic activity of SRP, FRP and MGP were monitored seasonally and metabolically-active communities were identified in oil-impacted sediments using quantitative real time Reverse Transcription -PCR and clone library analysis of key functional genes: Dissimilatory (bi)sulfite reductase (dsrAB), Geobactereceae-specific citrate synthase (gltA), methyl coenzyme M reductase (mcrA), and benzyl succinate synthase (bssA). In situ application of montmorillonite clay was assessed for its potential at accelerating PHC degradation by stimulating microbial activities. Levels of dsrA, gltA and bssA transcripts suggested that PHC-oxidizing SRP are more active in summer while FRP are more active in winter, indicating their activities linked to the seasonal changes of redox potential and vegetation. BssA gene expression peaked in winter, and was highest at more highly oil-impacted sites. Expression of all genes was higher in clay-amended sites. bssA transcript level and Fe(II) production were highest in clay-amended microcosm. Total petroleum hydrocarbon (TPH) levels were lower in oil and clay-amended microcosm incubation than one with oil only amendment, suggesting enhanced TPH degradation by clay amendment. Pyrosequencing analysis 16S rRNA gene in clay-amended microcosms demonstrated the highest percentage abundance of groups closely related to known anaerobic aromatic degraders. Levels of mcrA transcripts correlated with methane production rates in TE-amended coal bed production water enrichments. The findings of the present study clearly support the advantage of gene expression analyses for estimating microbial activity. To the best of our knowledge, this is the first in situ study which employs key functional gene markers as molecular proxies for metabolic activity and diversity assessments in anaerobic oil-contaminated salt marsh sediment and also elucidates clay-enhanced in situ TPH degradation.

Thesis (Ph. D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Biology; and the Woods Hole Oceanographic Institution), February 2002. / Vita. / Includes bibliographical references (leaves 153-161). / I used direct measurements of nitrogen gas (N₂ fluxes and a ¹⁵N stable isotope tracer to determine the contribution of denitrification to salt marsh sediment N cycling. Denitrification in salt marsh tidal creekbottoms is a major sink for groundwater nitrate of terrestrial origin. I studied creekbottom denitrification by direct measurements of N₂ fluxes in closed chambers against a low-N₂ background. I undertook experiments and simulation modeling of sediment N₂ fluxes in closed chambers to optimize the key experimental parameters of this approach. Denitrification in these sediments was driven by the degradation of labile organic matter pools which are depleted during long incubations. Sediment thickness was the most important parameter controlling the required incubation time. Errors of up to 13% with gas headspaces and 80% with water headspaces resulted from headspace N2 accumulation and the resulting collapse of the sediment-water diffusion gradient. These errors could be eliminated by using headspaces of sufficient thickness. Headspace flushing to reduce ammonium accumulation did not affect denitrification rates, but caused transient disturbance of N₂ flux rates. Direct measurements of 0₂, C0₂, N₂, and inorganic N fluxes from the sediments of a salt marsh tidal creek were made in order to examine the interaction of denitrification with the carbon, oxygen, and N cycles. Organic carbon concentration and lability were the primary controls on metabolic rates. C0₂/N flux ratios averaged 6.1, indicating respiration driven by algal biomass. / (cont.) Allochthonous denitrification accounted for 39% of total sediment denitrification (2.7 mol N m⁻² yr⁻¹). 46% of remineralized ammonium was denitrified, while the contribution of autochthonous denitrification to 0₂ and C0₂ fluxes was 18% and 10%, respectively. A ¹⁵N-ammonium tracer was used to study competition between plants and nitrifying bacteria for remineralized ammonium. In undisturbed sediments of Spartina alterniflora, plant uptake out-competed nitrification-denitrification, with plant uptake accounting for 66% of remineralized ammonium during the growing season. Under N fertilization (15.5 mol m⁻² yr⁻¹), both plant N uptake and denitrification increased, but denitrification dominated, accounting for 72% of the available N. When plant uptake was hydrologically suppressed, nitrification-denitrification was stimulated by the excess N, shifting the competitive balance toward denitrification. / by Michael Robert Hamersley. / Ph.D.

Previous research into bottom-up processes on saltmarshes has mainly focused on the influence of plant succession on herbivores. This study will present original research exploring the influence of bottom-up processes in a saltmarsh ecosystem between three trophic levels: Orchestia, redshanks, and sparrowhawks. Density dependence, may be the dominant top-down effect when higher numbers of sparrowhawks and redshanks are present, and may mask top-down and bottom-up trait effects which are constant. Bottom-up effects begin to emerge when cold conditions force redshanks from muddy creeks onto the saltmarsh to forage for Orchestia, because their primary prey, Corophium become less available. Larger flocks form and feeding on Orchestia requires them to balance a need to profit from the best available feeding patches and to be vigilant to sparrowhawk attack. Redshank vulnerability is compounded, because Orchestia hide in cold temperatures, so probing in the soil with their heads down makes them more vulnerable to sparrowhawk attack. Larger flocks may be able to exploit areas closer to sparrowhawk-concealing cover at the terrestrial boundary because they feel safer in greater numbers. Warmer temperatures make Orchestia more active which attracts redshanks, which can simultaneously feed and be vigilant because they peck and catch crawling and jumping Orchestia with their heads up. Consequently, increased flock size may temporarily depress Orchestia abundance, so that redshanks become spaced, leaving isolated individuals more vulnerable to attack. Therefore, it is a temperature-dependent bottom-up process which impacts upon both Orchestia and redshank behaviour, which then may influence the hunting success of sparrowhawks. Whether the characteristics of this saltmarsh ecosystem and the trophic dynamics can be compared to other examples is questionable. Saltmarshes probably differ in their topography and the way in which environmental conditions affect them that then defines which species are present and how these species interact.

Os objetivos deste estudo foram: 1) avaliar por meio da análise elementar (C, N, H, S, O), Ressonância Paramagnética Eletrônica (RPE), e Espectroscopia de Infravermelho com Transformada de Fourier (FTIR) às características dos ácidos húmicos (AHs) extraídos do solo do manguezal de Pai Matos (Cananéia-SP-BR) sob diferentes tipos de vegetação (Rhizophora mangle, Avicennia schaueriana, Laguncularia racemosa, Spartina alterniflora) e condições de redução, bem como às características dos AHs extraídos do solo de marismas espanholas também sob diferentes tipos de vegetação (Galícia: Spartina maritima, Juncus maritimus e Phragmites australis; Valência: Phragmites australis e Scirpus maritimus) e condições de redução; e 2) avaliar por meio da Pirólise-associada à cromatografia gasosa/espectrometria de massas (Pi-CG/EM) às características da matéria orgânica (MO) extraída do solo destes ecossistemas sob os mesmos tipos de vegetação e condições e redução. O carbono orgânico total, o nitrogênio total, o enxofre total e os valores de Eh e pH dos solos estudados também foram analisados. Os resultados mostraram que o ambiente predominantemente redutor dos solos estudados ocasionou uma maior incorporação de N e S e uma menor concentração de radicais livres semiquinona (CRLS) em relação aos AHs derivados de solos de ecossistemas terrestres, sugerindo desta forma, a presença de uma matéria orgânica (MO) menos humificada no solo das áreas de estudo; entretanto, somente os AHs extraídos do solo do manguezal apresentaram uma maior razão atômica H/C e O/C em relação aos AHs derivados de solos terrestres. A CRLS também foi maior nos AHs das camadas subsuperficiais dos solos estudados, sugerindo que o ambiente mais anóxico ocasionou um maior grau de humificação da MOS destas camadas, entretanto, como os manguezais e as marismas são ambientes de sedimentação, a contribuição de uma matéria orgânica mais humificada de origem terrestre previamente depositada nas áreas de estudo também pode ter ocasionado este resultado. Entre as diferentes vegetações estudadas, foi observada uma maior relação C/N e CRLS dos AHs extraídos do solo sob Rhizophora e Avicennia (manguezal) e uma maior relação C/N dos AHs extraídos do solo da marisma de Valência-ES, sugerindo uma maior recalcitrância da MOS sob estas vegetações. Os espectros de FTIR não permitiram verificar variações significativas na composição química e estrutural dos AHs obtidos dos solos sob as diferentes vegetações e condições de redução; entretanto, observo-se através da Pi-CG/EM uma maior concentração de compostos lignificados na MO extraída do solo sob Avicennia e Rhizophora (manguezal) e da marisma de Valência-ES, também sugerindo uma maior recalcitrância e menor humificação da MOS destas áreas de estudo. Além disso, observou-se que a maior decomposição da MOS ocasionou o decréscimo no conteúdo de lignina com o acréscimo relativo de compostos alifáticos; o acréscimo dos compostos alifáticos de cadeia curta em detrimento ao acúmulo dos de cadeia longa; a perda da dominância dos alcanos/alquenos de cadeias ímpares em relação aos de cadeias pares; e o acréscimo de compostos orgânicos de origem microbiana nos solos estudados. / The goals of this study were: 1) evaluate by elemental analysis (C, N, H, S, O), Electronic Paramagnetic Resonance (EPR), and Fourier transformed infrared spectroscopy (FTIR) the characteristics of the humic acids (HA) extracted from the soil of Pai Matos mangrove forest (Cananéia, SP, BR) under different vegetation types (Rhizophora mangle, Avicennia schaueriana, Laguncularia racemosa, Spartina alterniflora) and soil reduction conditions, as well as the HA extracted from Spanish salt marsh soils (Galícia and Valência) under different vegetation types (Galicia: Spartina maritima, Juncus maritimus, and Phragmites australis; Valencia: Phragmites australis e Scirpus maritimus) and soil reduction conditions; and 2) evaluate by Pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) technique the characteristics of the SOM extracted from the these study areas under the same vegetation types and reduction condition. The total organic carbon, the total nitrogen, the total sulfur, and the soil pH and Eh in the field also were analyzed. The results showed that the predominantly anoxic soil condition caused the higher N and S content in the HA of both study areas than the HA derived from terrestrial soils and marine environments, and that the studied HA also showed a lower semiquinone-type free radical (SFR) content than terrestrial soils, thus suggesting a lower humification degree of the SOM derived from the study areas; however, only the HA derived from de mangrove forest soil showed a higher H/C and O/C atomic ratio than the HA derived from the terrestrial soils and marine environments. In addition, the SFR content also was lower in the surface soil layers, suggesting the input of a fresh and labile plant-derived organic matter as responsible by the lower humification degree of the SOM derived from these layers; however, as mangroves and salt marshes are sedimentation environments, it is likely that besides the more anoxic soil condition, a more humified organic matter (OM) derived from terrestrial soils also may cause the highest SFR content observed in the subsurface soil layers. Among the different vegetation types, also was observed a higher C/N ratio of the soil and HA derived from Valencian salt marsh, as well as both a higher C/N ratio and SFR content in the HA extracted from the soil under Rizhophora and Avicennia (mangrove), suggesting a more recalcitrant SOM in these sites. The FTIR spectras did not show significant variation among vegetation types and soil depths, and finally, by the Py-GC/MS technique was observed that the OM derived from the Valencian salt marsh soils and from the soil under Avicennia and Rhizophora (mangrove) showed a more recalcitrant SOM (higher lignin content) than the SOM derived from the soil under the other vegetation types studied. In addition, it was also observed that the SOM decomposition caused a decrease in lignin contents and a relative increase in aliphatics; an increase in short-chain aliphatics at the expense of longer ones; a loss of odd-over-even dominance in the n-alkanes and n-alkenes; and an increase in microbial products in the studied soils.

Os objetivos deste estudo foram: 1) avaliar por meio da análise elementar (C, N, H, S, O), Ressonância Paramagnética Eletrônica (RPE), e Espectroscopia de Infravermelho com Transformada de Fourier (FTIR) às características dos ácidos húmicos (AHs) extraídos do solo do manguezal de Pai Matos (Cananéia-SP-BR) sob diferentes tipos de vegetação (Rhizophora mangle, Avicennia schaueriana, Laguncularia racemosa, Spartina alterniflora) e condições de redução, bem como às características dos AHs extraídos do solo de marismas espanholas também sob diferentes tipos de vegetação (Galícia: Spartina maritima, Juncus maritimus e Phragmites australis; Valência: Phragmites australis e Scirpus maritimus) e condições de redução; e 2) avaliar por meio da Pirólise-associada à cromatografia gasosa/espectrometria de massas (Pi-CG/EM) às características da matéria orgânica (MO) extraída do solo destes ecossistemas sob os mesmos tipos de vegetação e condições e redução. O carbono orgânico total, o nitrogênio total, o enxofre total e os valores de Eh e pH dos solos estudados também foram analisados. Os resultados mostraram que o ambiente predominantemente redutor dos solos estudados ocasionou uma maior incorporação de N e S e uma menor concentração de radicais livres semiquinona (CRLS) em relação aos AHs derivados de solos de ecossistemas terrestres, sugerindo desta forma, a presença de uma matéria orgânica (MO) menos humificada no solo das áreas de estudo; entretanto, somente os AHs extraídos do solo do manguezal apresentaram uma maior razão atômica H/C e O/C em relação aos AHs derivados de solos terrestres. A CRLS também foi maior nos AHs das camadas subsuperficiais dos solos estudados, sugerindo que o ambiente mais anóxico ocasionou um maior grau de humificação da MOS destas camadas, entretanto, como os manguezais e as marismas são ambientes de sedimentação, a contribuição de uma matéria orgânica mais humificada de origem terrestre previamente depositada nas áreas de estudo também pode ter ocasionado este resultado. Entre as diferentes vegetações estudadas, foi observada uma maior relação C/N e CRLS dos AHs extraídos do solo sob Rhizophora e Avicennia (manguezal) e uma maior relação C/N dos AHs extraídos do solo da marisma de Valência-ES, sugerindo uma maior recalcitrância da MOS sob estas vegetações. Os espectros de FTIR não permitiram verificar variações significativas na composição química e estrutural dos AHs obtidos dos solos sob as diferentes vegetações e condições de redução; entretanto, observo-se através da Pi-CG/EM uma maior concentração de compostos lignificados na MO extraída do solo sob Avicennia e Rhizophora (manguezal) e da marisma de Valência-ES, também sugerindo uma maior recalcitrância e menor humificação da MOS destas áreas de estudo. Além disso, observou-se que a maior decomposição da MOS ocasionou o decréscimo no conteúdo de lignina com o acréscimo relativo de compostos alifáticos; o acréscimo dos compostos alifáticos de cadeia curta em detrimento ao acúmulo dos de cadeia longa; a perda da dominância dos alcanos/alquenos de cadeias ímpares em relação aos de cadeias pares; e o acréscimo de compostos orgânicos de origem microbiana nos solos estudados. / The goals of this study were: 1) evaluate by elemental analysis (C, N, H, S, O), Electronic Paramagnetic Resonance (EPR), and Fourier transformed infrared spectroscopy (FTIR) the characteristics of the humic acids (HA) extracted from the soil of Pai Matos mangrove forest (Cananéia, SP, BR) under different vegetation types (Rhizophora mangle, Avicennia schaueriana, Laguncularia racemosa, Spartina alterniflora) and soil reduction conditions, as well as the HA extracted from Spanish salt marsh soils (Galícia and Valência) under different vegetation types (Galicia: Spartina maritima, Juncus maritimus, and Phragmites australis; Valencia: Phragmites australis e Scirpus maritimus) and soil reduction conditions; and 2) evaluate by Pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) technique the characteristics of the SOM extracted from the these study areas under the same vegetation types and reduction condition. The total organic carbon, the total nitrogen, the total sulfur, and the soil pH and Eh in the field also were analyzed. The results showed that the predominantly anoxic soil condition caused the higher N and S content in the HA of both study areas than the HA derived from terrestrial soils and marine environments, and that the studied HA also showed a lower semiquinone-type free radical (SFR) content than terrestrial soils, thus suggesting a lower humification degree of the SOM derived from the study areas; however, only the HA derived from de mangrove forest soil showed a higher H/C and O/C atomic ratio than the HA derived from the terrestrial soils and marine environments. In addition, the SFR content also was lower in the surface soil layers, suggesting the input of a fresh and labile plant-derived organic matter as responsible by the lower humification degree of the SOM derived from these layers; however, as mangroves and salt marshes are sedimentation environments, it is likely that besides the more anoxic soil condition, a more humified organic matter (OM) derived from terrestrial soils also may cause the highest SFR content observed in the subsurface soil layers. Among the different vegetation types, also was observed a higher C/N ratio of the soil and HA derived from Valencian salt marsh, as well as both a higher C/N ratio and SFR content in the HA extracted from the soil under Rizhophora and Avicennia (mangrove), suggesting a more recalcitrant SOM in these sites. The FTIR spectras did not show significant variation among vegetation types and soil depths, and finally, by the Py-GC/MS technique was observed that the OM derived from the Valencian salt marsh soils and from the soil under Avicennia and Rhizophora (mangrove) showed a more recalcitrant SOM (higher lignin content) than the SOM derived from the soil under the other vegetation types studied. In addition, it was also observed that the SOM decomposition caused a decrease in lignin contents and a relative increase in aliphatics; an increase in short-chain aliphatics at the expense of longer ones; a loss of odd-over-even dominance in the n-alkanes and n-alkenes; and an increase in microbial products in the studied soils.

Die Anpassung von Küstenniederungen, Seemarschen und Inseln an klimatische Veränderungen und einen steigenden Meeresspiegel ist eine der großen Herausforderungen des 21. Jahrhunderts. Im Gegensatz zu eingedeichten Küstenmarschen und Inseln besitzen tidebeeinflusste Seemarschen ein natürliches Anpassungspotential an sich verändernde hydrologische Rahmenbedingungen. Überflutungsabhängige Sedimenteinträge führen zu einem Anwachsen der Marschoberfläche und kompensieren somit einen Anstieg des Meeresspiegels. Die 10 verbliebenen nordfriesischen Halligen (Schleswig-Holstein, Deutschland) (Kapitel 1) sind bewohnte Inselmarschen, welche aufgrund ihrer anthropogenen Überprägung von naturbelassenen Seemarschen unterschieden werden müssen. Diese umfasst z.B. den Bau von flachen Sommerdeichen und Sielanlagen. Inwiefern sich diese Veränderungen auf die Sedimentdynamik der Marschen auswirken, ist bislang unzureichend belegt, was eine fundierte Diskussion bezüglich zukünftiger Entwicklungsperspektiven der Halligmarschen verhindert. Die vorliegende Arbeit ist dazu angelegt, diese Wissenslücke zu schließen. Sie untersucht das vertikale Marschwachstum exemplarisch auf den Halligen Hooge, Langeneß und Nordstrandischmoor und beurteilt deren Anpassungsvermögen an einen steigenden Meeresspiegel. Darüber hinaus werden zukünftige Entwicklungsperspektiven diskutiert. Um Faktoren und Prozesse, welche maßgeblich die Sedimentdynamik der Halligen beeinflussen, messbar zu machen, musstengeeignete Methoden entwickelt und angewendet werden. Zur Berechnung jährlicher Überflutungshäufigkeiten wurden Pegelschwellenwerte für Überflutungsereignisse auf Basis von digitalen Geländemodellen (DGMs) und d-GPS (differential global positioning system) Messungen errechnet und auf die verfügbaren, regionalen Pegeldaten angewendet (Kapitel 2). Sedimentfallen, bestehend aus LDPE (low density polyethylene) Flaschen (1 Liter) und Kunstrasenmatten (20 x 30 cm), bilden die Grundlage einer dreijährigen (November 2010 – März 2013) Feld- und Laborstudie zur zeitlichen und räumlichen Erfassung der sturmflutabhängigen Sedimentdeposition (Kapitel 3). Durch die Verwendung regionaler bodenphysikalischer Parameter (Lagerungsdichte und Gehalt an organischer Bodensubstanz) können Depositionsraten in eine vertikale Wachstumskomponente transformiert werden. Dazu werden Ergebnisse einer Rammkernsondierung genutzt, welche 12 Sedimentkerne mit einer Länge von maximal 100 cm umfasst. Die Sedimentbohrkerne sind weiterhin die Grundlage für eine 137Cs- und 210Pb-Datierung. Die Kombination beider Datensätze (Kapitel 4) ermöglicht einen schlüssigen Vergleich der Marschentwicklung seit dem Jahr 1915 mit regionalen Pegeldaten und Projektionen des zukünftigen Meeresspiegelanstiegs. Die Analyse der verfügbaren Pegeldaten (Kapitel 2) zeigt eine hohe Variabilität der jährlichen Überflutungshäufigkeiten. Das zehnjährige Mittel eintretender Ereignisse beträgt 2 Überflutungen auf Hooge, 9 – 10 Überflutungen auf Langeneß und 15 Überflutungen auf Nordstrandischmoor. Aufgrund der künstlichen Überhöhung der Marschkante durch Sommerdeiche mit + 1.54 m ü. mittlerem Tidehochwasser (MThw) auf Hooge und + 0.98 m ü. MThw auf Langeneß werden die betreffenden Halligen lediglich bei selten eintretenden Sturmflutereignissen überflutet. Die Höhe des wasser- und sedimentundurchlässigen Steinpflasters auf Nordstrandischmoor beträgt lediglich + 0.7 m ü. MThw. Die methodischen Untersuchungen bezüglich der Nutzung von Sedimentfallen (Kapitel 3) zeigen, dass beide Typen von Sedimentfallen vergleichbare Ergebnisse liefern. Oberhalb einer Depositionsrate von ~ 2.0 kg/m2 sinkt das Rückhaltevermögen der Kunstrasenmatte im Vergleich zur LDPE Flasche deutlich ab. Die parallele Nutzung beider Fallentypen, insbesondere wenn die Depositionsraten den Schwellenwert (~ 2.0 kg/m2) nicht überschreiten, erlaubt: (1) Die Überprüfung, ob beide Datensätze konsistent sind. (2) Die Identifizierung von Ausreißern. (3) Eine Abschätzung, ob Sediment auf oder in der Sedimentfalle nach der Überflutung remobilisiert wird. Um die Sedimentdeposition in eine vertikale Wachstumsrate zu übersetzen, muss die mittlere Bodendichte als auch der Gehalt an organischer Bodensubstanz des Marschbodens berücksichtigt werden. Die Bohrkernuntersuchungen zeigen, dass diese bodenphysikalischen Parameter auf den unterschiedlichen Halligen stark variieren. Marschen, die häufig überflutet werden lagern weniger organisches Material im Oberboden ein als selten überflutete Marschen. Niedrige Gehalte an leichten organischen Materialien geringer Dichte resultieren wiederum in einer höheren Lagerungsdichte des Marschbodens (Hooge 0.64 g/cm3, Langeneß 0.67 g/cm3, Nordstrandischmoor 0.83 g/cm3). Autochthones organisches Material (welches primär von der Halligvegetation stammt) trägt mit einem Anteil von 9.0 ± 1.4 % (Hooge) bis 21.4 ± 6.6 % (Nordstrandischmoor) zum Marschwachstum bei. Die Ergebnisse der Sedimentfallenuntersuchungen als auch der Datierungen zeigen deutlich ein Ungleichgewicht zwischen Marschwachstum und Meeresspiegelanstieg seit Beginn des 20. Jahrhunderts. Die langjährigen Wachstumsraten, basierend auf der 210Pb-Datierung, liegen mit 1.0 ± 0.3 mm/a auf Hooge, 1.2 ± 0.3 mm/a auf Langeneß und 2.6 ± 0.9 mm/a auf Nordstrandischmoor deutlich unterhalb des MThw-Anstiegs von 5.0 ± 0.3 mm/a (1951 – 2011, Wyk auf Föhr). Projektionen des Meeresspiegelanstiegs bis zum Jahr 2100 (Berechnet durch das fwu, Siegen) weisen darauf hin, dass extreme Wasserstände (höchste, jährliche Tidehochwasserstände, HThw, 6.6 ± 3.8 mm/a) deutlich schneller ansteigen werden als das MThw oder der mittlere Meeresspiegel (2.6 ± 0.4 mm/a). Aufgrund dieser Beobachtungen ist von einem zukünftigen Anstieg des Gefährdungspotentials für die Halligen auszugehen, wenn es nicht gelingt, ein sedimentologisches Gelichgewicht zwischen Meeresspiegel und Marschwachstum herzu stellen. Der Anstieg der Wellenhöhe und Periode, aufgrund von steigender Wassertiefe und einer geringeren Wellentransmissionsrate an den Sommerdeichen, resultiert in einer steigenden hydrodynamischen Belastung der Warften und der Marschoberfläche. Das sedimentäre Ungleichgewicht, besonders auf Hooge und Langeneß, kann eindeutig auf das hydrologische Management der Halligen zurückgeführt werden. Aus sedimentologischer Sicht sind die beiden Hauptkritikpunkte (1) die geringe Anzahl an Überflutungen aufgrund der Deichanlagen und (2) der eingeschränkte Transport suspendierter Feststoffe über die Binnenpriele. Letzteres resultiert aus der Blockade der Binnenpriele durch Sielanlagen und führt zu einer Abnahme der Sedimentdeposition mit zunehmender Entfernung zur Uferlinie. Um dem Ungleichgewicht zwischen Marschwachstum und Meeresspiegelanstieg entgegenzuwirken, ist es dringend erforderlich, neue Managementstrategien für die Halligen zu entwickeln (Kapitel 6), welche sedimentologische/geomorphologische Aspekte sowie die speziellen Bedürfnisse der Halligbevölkerung gleichermaßen berücksichtigen. Letztere beinhalten die Minimierung ökonomischer Schäden wie etwa Einschränkungen der landwirtschaftlichen Nutzung oder des Tourismus. Mögliche Szenarien können ein Abflachen der bestehenden Deiche oder deren Rückbau und Erneuerung durch wasser- und sedimentdurchlässige Rauhstreifen (z.B. Elastocoast®, BASF) beinhalten. Weiterhin erscheint die Reaktivierung der blockierten Binnenpriele eine plausible Maßnahme zu sein. Erste Freilandexperimente auf Hallig Langeneß (Kapitel 5) belegen einen erhöhten Sedimenttransport in die Binnenmarsch aufgrund geöffneter Sielanlagen in Verbindung mit Windstau (Thw-Ereignisse über Springtideniveau). Ob die generelle Umsetzung derartiger Maßnahmen möglich ist, ist in erster Linie davon abhängig, ob die Halligbevölkerung derartigen Veränderungen ihrer Umwelt aufgeschlossen gegenübersteht und diese lokalpolitisch getragen werden. Auf jeden Fall ist ein Umdenken dahingehend erforderlich, die halligtypischen Überflutungen (Land-Unter) nicht ausschließlich als Bedrohung zu verstehen. Sie sind ein natürliches Phänomen, welches notwendig ist, um das Gleichgewicht zwischen Meeresspiegelanstieg und Sedimentdeposition aufrechtzuerhalten.

The spatial and temporal patterns in the ichthyofaunal community composition and structure in Taylor’s salt marsh and adjacent eelgrass beds (Zostera capensis) in the Kariega Estuary, was investigated every two months between May 2006 and March 2007. Total ichthyofaunal abundances and biomass in the salt marsh ranged between 0.55 and 21.7 ind.10m-2 and between 0.03 and 1.9 g.wwt.10m⁻², respectively. There were no significant spatial patterns in the values evident (P > 0.05 in all cases) although seasonal trends were marked, with highest values consistently recorded during the warmer summer months. Investigations into the community structure showed that the ichthyofaunal community within salt marsh was composed almost exclusively of juveniles of estuarine dependant (category II) species, mainly juvenile Mugilidae (<20mm SL) that comprised up to 83% of all fish sampled. Hierarchical cluster analysis and multidimensional scaling did not identify any distinct spatial patterns in the ichthyofaunal community within the salt marsh. The absence of any spatial patterns in the community structure could be related to the absence of any significant spatial patterns in the physico-chemical (temperature, salinity and dissolved oxygen concentrations) and biological (water column and microphytobenthic algal concentrations) variables within the salt marsh (P > 0.05 in all cases). Temporal shifts in the ichthyofaunal community structure within the salt marsh were, however, evident largely reflecting the breeding cycles of individual species within the sub-region. Within the adjacent eelgrass beds, total ichthyofaunal abundances and biomass ranged between 8.4 and 49.4 ind.10m⁻² and between 2.9 and 94.5 g.wwt.10m⁻², respectively. Once again there were no distinct spatial patterns in the abundance and biomass values evident although seasonal patterns were marked. In contrast to the salt marsh, within the in the eelgrass community, there were a large number of adult individuals recorded. Again category II species, the estuarine dependent species, were numerically and gravimetrically dominant. The dominance of category II species reflects the marine dominance of Kariega Estuary. The remaining estuarine utilisation categories did not contribute significantly to abundance or standing stock totals. Hierarchical cluster analysis showed that the salt marsh and eelgrass beds represented two distinct habitats within the Kariega Estuary. Within the salt marsh, the family Mugilidae were numerically dominant contributing 83% of the total catch. Within the eelgrass beds, the sparid, Rhabdosargus holubi and representatives of the family Gobidae contributed 36.3% and 33.9% respectively to the total catch. Estuaries with a wide range of microhabitats have been demonstrated to support a more diverse ichthyofaunal community. Shallow water habitats in general are important areas for juvenile fish within estuaries. Taylor’s salt marsh provides an alternative shallow water habitat, occupied by a distinct ichthyofaunal community composition, with increased food availability and decreased predation pressure, for a wide range of fish species.

Les prés-salés sont des interfaces complexes entre le milieu continental et maritime d'une grande importance environnementale. En contexte de changement climatique, ces zones vulnérables font maintenant l'objet d'une attention particulière. Cette étude porte sur la mesure et la compréhension de la dynamique des prés-salés soumis à un régime méga-tidal (marnage supérieur à 10 m), à travers l'étude de deux estuaires des côtes du Nord-Ouest de la France : l'estuaire du Couesnon en baie du Mont-Saint-Michel (MSM) et l'estuaire de la Somme. L'originalité de l'approche est de considérer une très large gamme d'échelles temporelles, depuis l'échelle séculaire jusqu'à l'événement de marée. A l'échelle séculaire, la mesure de l'évolution de la surface des prés salés en Baie de Somme, à partir de documents historiques et de photos aériennes, montre une évolution étonnamment similaire à celle de l'estuaire du Couesnon depuis la canalisation de la partie terminale de ces rivières au milieu du XIXème siècle. Après une phase de développement rapide des prés salés, une réduction concomitante des taux de croissance est observée dans les deux estuaires au début du XXème siècle. Nous proposons que l'augmentation du niveau marin à cette période soit responsable de cette réduction de croissance des prés-salés. L'analyse pluriannuelle des surfaces en baie de Somme met en évidence des périodes d'érosion des prés-salés résultant de la migration des chenaux tidaux à la frontière des prés-salés. Une étude sur 3 ans en baie du MSM a permis de caractériser le fonctionnement d'un chenal tidal sinueux en frontière des prés salés, grâce à des mesures topographiques inédites réalisées avec un Lidar terrestre haute résolution, combinées à des mesures hydro-sédimentaires. Des relevés journaliers durant les marées d'équinoxe de Septembre et de Mars ont mis en évidence une relation très non-linéaire entre érosion des berges et hauteur de marée. Cette relation est régie principalement par une forte augmentation des vitesses du courant lors du jusant. L'étude des taux d'accrétion dans la partie interne du méandre tidal a révélé l'importance de la présence de la végétation pionnière. Des lois empiriques de sédimentation et d'érosion en fonction de la hauteur de marée sont proposées. Il est montré que 6 % des marées les plus hautes de l'année sont responsables de 90 % de l'érosion des prés-salés par migration du chenal, alors que la sédimentation est dominée par les marées les plus fréquentes. Ces travaux mettent en évidence le rôle fondamental de la divagation des chenaux en marge des prés salés méga-tidaux. Leur dynamique est contrôlée principalement par les marées les plus importantes, dont la fréquence est régit par des cycles nodaux de 4,4 à 18,6 ans. Les données topographiques et hydro-sédimentaires inédites acquises durant ce travail de thèse fournissent des contraintes fortes pour le développement de modèle d'évolution des prés salés. / Salt marshes are complex interfaces between land and sea of high environmental values. Theses vulnerable landforms are now subjected to particular attention since they are very sensitive to sea level changes and storm events. This study focuses on salt marsh dynamic subjected to a mega-tidal range (higher than 10 m) that received less attention, through the study of two estuaries of the north-western coast of France: The Couesnon estuary in the Bay of Mont -Saint-Michel and the Somme estuary. The originality of this study is to consider a wide range of time scales, from the secular one to tide events. At secular time scale, we measure the evolution of salt marsh surfaces in the Somme bay, from historical documents and aerial photographs. The comparison with the Couesnon estuary reveals a surprisingly similar evolution of salt marsh extension, since the derivation and embankment of their main river in early mid XIXth century. After a period of rapid salt marsh extension, a concomitant reduction of growth rates is observed in both estuaries in the early XXth century. We propose that the increase in sea level rise during this period is responsible for the reduced growth of salt marshes. The pluriannual analysis highlights periods of erosion of salt marshes resulting from the tidal channels migration on salt marsh fringe. However, the pluriannual time scale does not capture the processes governing the dynamics of channel migration. This has motivated a 3 years study of a sinuous tidal channel located on the fringe of the marsh with the aim to understand its dynamics at daily to annual scales. We have acquired 36 high resolution topographic surveys with Terrestrial Laser Scanner. We used daily surveys during spring tides of various High Water Level (HWL) to analyze channel dynamics at tidal event timescales. Daily TLS measurements revealed a non-linear relationship between bank erosion and tidal height. This relationship is related to a sharp increase in flow velocities during the ebb (up to 2.2 m/s) for overmarsh tides. Spatially averaged accretion on the inner bend tends to increase linearly with HWL and is increased by a factor 2 during summer/autumn spring tides at the peak of pioneer vegetation development. We propose empirical laws of sedimentation and erosion depending on the tide height, which model that 6% of the highest tides of the year are responsible for 90 % of salt marsh erosion by channel migration, while sedimentation is dominated by frequent tides. These results highlight the need to incorporate the influence of vegetation on sedimentation rates as well as tidal migration processes in models of salt marsh dynamic in mega-tidal environment. Long term modeling should account for the highest tides of the year, that make tidal channel very sensitive to the nodal cycle (18.6 years) which controls the occurrence of the highest tides in mega-tidal environment.

Climate change and increasing sea level elevation are predicted to increase salinity in estuarine tidal wetlands in the Southeastern United States. Since much of the ecosystem function in these areas is predicated upon salinity regimes, many fundamental changes are likely to occur as a result. The influence of salinity and SO4 2- reduction on PO4 3- release from tidal wetland soils was evaluated along a salinity gradient at three sites in The St. John’s River, Florida using both field and laboratorybased methods. Porewater was sampled over the course of 10 months to determine ambient levels of SO4 2- and PO4 3-. Lab-based experiments, soils samples were subjected to seawater and SO4 2- treatments in an attempt to induce PO4 3- release. Salinity was lowest at Sixmile Creek (0.45 ± 0.1 g kg-1) and Goodby’s Creek (2.05 ± 2.3 g kg-1) and much higher at Sister’s Creek (27.81 ± 3.1 g kg-1). The organic content of soils was highest (82.35% ± 5.11) at Sixmile Creek, intermediate at Goodby’s Creek (64.45% ± 7.02) and lowest at Sister’s Creek (32.11% ± 9.61). Total soil P was highest at the freshwater Sixmile Creek (1101.64 ± 220.2 μg g-1), intermediate at the brackish Goodby’s Creek (719.61 ± 114.3 μg g-1) and lowest at the Sister’s Creek saltmarsh (475.85 ± 110.9 μg g-1). Porewater PO4 3- was higher at Sixmile and Goodby’s Creek sites (9.44 ± 15.6, 8.99 ± 14.7 !g L-1, respectively) compared to Sister’s Creek (0.6 ± 3.1 !g L-1). Porewater SO4 2- was lower at Sixmile (70.73± 57.58 !g L-1) and Goodby’s Creeks (124.35 ± 152.5 !g L-1) compared to Sister’s Creek (1931.41 ± 557.82 !g L-1). Temporal and spatial trends indicated that SO4 2- and PO4 3- in porewater was likely due to floodwater content and that direct reaction between analytes in soils was unlikely. The addition of aerated seawater failed to cause PO4 3- release from any sites. The incubation of soils under anaerobic conditions, in the presence of Na2SO4 induced SO4 2- reduction, but inhibited PO4 3- flux from both Sixmile and Goodby’s Creek, which is attributed here to likely S- toxicity (Roychoudhury et al., 1999). PO4 3- flux from Sister’s Creek increased in association with Na2SO4 concentration, likely due to more Fe availability to mitigate Stoxicity. Ambient seawater additions to soils under anaerobic conditions followed a similar trend, but the results were not statistically conclusive. Overall, both field and labbased data indicated that Tidal wetland porewater PO4 3- likely originates from floodwaters and that increased salinity and SO4 2- reduction did not directly enhance soil PO4 3- fluxes.

Saltmarshes provide a diverse range of ecosystem services and are protected under a number of international designations. Nevertheless they are generally declining in extent in the United Kingdom and North West Europe. The drivers of this decline are complex and poorly understood. When considering mitigation and management for future ecosystem service provision it will be important to understand why, where, and to what extent decline is likely to occur. Few studies have attempted to forecast saltmarsh morphodynamics at a system level over decadal time scales. There is no synthesis of existing knowledge available for specific site predictions nor is there a formalised framework for individual site assessment and management. This project evaluates the extent to which machine learning model approaches (boosted regression trees, neural networks and Bayesian networks) can facilitate synthesis of information and prediction of decadal-scale morphological tendencies of saltmarshes. Importantly, data-driven predictions are independent of the assumptions underlying physically-based models, and therefore offer an additional opportunity to crossvalidate between two paradigms. Marsh margins and interiors are both considered but are treated separately since they are regarded as being sensitive to different process suites. The study therefore identifies factors likely to control morphological trajectories and develops geospatial methodologies to derive proxy measures relating to controls or processes. These metrics are developed at a high spatial density in the order of tens of metres allowing for the resolution of fine-scale behavioural differences. Conventional statistical approaches, as have been previously adopted, are applied to the dataset to assess consistency with previous findings, with some agreement being found. The data are subsequently used to train and compare three types of machine learning model. Boosted regression trees outperform the other two methods in this context. The resulting models are able to explain more than 95% of the variance in marginal changes and 91% for internal dynamics. Models are selected based on validation performance and are then queried with realistic future scenarios which represent altered input conditions that may arise as a consequence of future environmental change. Responses to these scenarios are evaluated, suggesting system sensitivity to all scenarios tested and offering a high degree of spatial detail in responses. While mechanistic interpretation of some responses is challenging, process-based justifications are offered for many of the observed behaviours, providing confidence that the results are realistic. The work demonstrates a potentially powerful alternative (and complement) to current morphodynamic models that can be applied over large areas with relative ease, compared to numerical implementations. Powerful analyses with broad scope are now available to the field of coastal geomorphology through the combination of spatial data streams and machine learning. Such methods are shown to be of great potential value in support of applied management and monitoring interventions.