Nos bancos de dados de T. cruzi há cerca de 3.750 ESTs de amastigotas e tripomastigotas, seqüenciadas a partir das extremidades 5\' ou 3\' de clones de cDNA. A metodologia ORESTES (Open Reading Frame Expressed Sequence Tags) possibilita obter seqüências transcritas parciais derivadas majoritariamente da porção central dos mRNAs, favorecendo a descoberta de novos genes. Neste trabalho, caracterizamos ORESTES de formas infectantes amastigotas e tripomastigotas da cepa humana VL10 (ATVL). A metodologia foi padronizada com formas epimastigotas da cepa CL Brener (ECL), monitorando-se nas preparações de mRNA a contaminação por DNA e a integridade dos transcritos. Populações de cDNA foram obtidas utilizando-se diferentes iniciadores aleatórios. O mesmo iniciador foi empregado nas etapas de RT e PCR, realizada em condições de baixa estringência. Obtivemos 776 e 1522 ORESTES de ECL e ATVL, respectivamente. Após análise com o programa PHRED, aceitaram-se 745 ORESTES de ECL e 1476 de ATVL. As ORESTES apresentaram um tamanho médio de 680 pb e um conteúdo de G+C de 53%. O agrupamento com CAP3 gerou 463 agrupamentos de ECL (360 singletons e 103 contigs) e 454 de ATVL (337 singletons e 117 contigs). A anotação foi feita utilizando-se o programa BLAST contra o banco nr do NCBI. Na biblioteca de ATVL observamos um número elevado de seqüências de RNA ribossômico (27%), amplificadas preferencialmente por dois iniciadores. Para ECL, a contaminação por rRNA foi de 3,6%. Para cerca de 50% das ORESTES de ATVL (n= 729) foi encontrada similaridade em bancos de dados de proteínas. Destas, 316 apresentaram similaridade com proteínas putativas conhecidas e 413 foram anotadas como proteínas hipotéticas e hipotéticas conservadas. Para 87 ORESTES de ATVL (5,9%) não foi observada nenhuma similaridade. 628 ORESTES de ECL (84%) apresentaram similaridade com proteínas depositadas em bancos públicos, ao passo que nenhuma similaridade foi encontrada para 18 cDNAs (2,4%). Ensaios de Southern blot confirmaram a presença de 4 ORESTES no match analisadas nos genomas das duas cepas. Não puderam ser atribuídas a processos biológicos conhecidos 39% e 68% das seqüências dos contigs de ECL e ATVL, respectivamente. Nos processos de Proteólise e Peptidólise, estão incluídas 11% das ORESTES de ECL e 0,3% das ORESTES de ATVL. Outras diferenças funcionais putativas foram observadas. A abundância diferencial dos transcritos de algumas ORESTES foi analisada por northern blot nos estágios evolutivos das cepas. Southern blot do contig ATVL95 originou um padrão de hibridização com múltiplas bandas, característico de seqüências repetitivas. Este contig corresponde ao transcrito do DNA satélite de 195 pb (195 SAT), uma seqüência repetitiva que perfaz cerca de 10% do genoma de T. cruzi e cuja transcrição é controversa na literatura. A transcrição do 195 SAT foi comprovada por experimentos de + northern blot e por RT-PCR. Transcritos de 195 SAT foram detectados nas frações de RNA de poliA+ e poliA-. Esses transcritos não conteriam a seqüência SL, presente nos mRNAs de tripanossomatídios. e poliA Utilizando oligonucleotídios complementares às duas fitas de 195 SAT concluímos que ambas são transcritas. Embora esteja claro que 195 SAT é transcrito, sua função biológica permanece desconhecida. / In T. cruzi databases, aproximately 3.750 ESTs of amastigotes and trypomastigotes, sequenced from the 3´ or 5´ ends cDNA clones can be found in T. cruzi databases. The ORESTES (Open Reading Frame Expressed Sequence Tags) methodology generates partial transcribed sequences derived mainly from the central portions of mRNAs, favoring the discovery of new genes. In this work, we have characterized ORESTES sequences from the infective amastigote and trypomastigote forms of the human strain VL10 (ATVL). The methodology was standardized with epimastigotes of the CL Brener strain (ECL), monitoring in the mRNA population DNA contamination and the integrity of the transcripts. cDNA populations were obtained using different arbitrarily selected, nondegenerate primers. The same primer was used in the RT and PCR steps, performed under low-stringency conditions. We obtained 776 and 1522 ORESTES of ECL and ATVL, respectively. After analysis with PHRED program, 745 ORESTES of ECL and 1476 of ATVL were accepted for further characterization. ORESTES showed a medium size of 680 bp and a G+C content of 53%. Clustering with CAP3 generated 463 unique sequences of ECL (360 singletons and 103 contigs) and 454 of ATVL (337 singletons and 117 contigs). The annotation was made with BLAST program against the NCBI nr database. In the ATVL library we observed an elevated number of ribosomal RNA sequences (27%), amplified mainly by two primers. In the ECL library, rRNA contamination was about 3.6%. Approximately 50% of the ATVL sequences (n= 729) were found in protein databases. From these, 316 showed similarity with putative known proteins and 413 were annotated as hypothetical proteins and hypothetical conserved proteins. No hit was observed for 87 ORESTES of ATVL (5.9%). 628 ORESTES of ECL (84%) showed similarity with proteins in public databases, while for 18 cDNAs (2.4%) no similarity was found. Southern blot assays confirmed the presence of four no match analyzed ORESTES in the genomes of the two strains. No known biological process could be assigned to 39% and 68% of the sequences of ECL and ATVL contigs, respectively. In Proteolysis and Peptidolysis processes 11% and 0.3% of ECL and ATVL ORESTES were allocated, respectively. Additional putative functional differences were observed. The differential abundance of transcripts of some ORESTES was analyzed by northern blot assays in the developmental stages of the strains. Southern blot of the contig ATVL95 originated a hybridization pattern with multiple bands, characteristic of repetitive sequences. This contig corresponds to the transcript of the 195 bp satellite DNA (195 SAT), a repetitive sequence that accounts for 10% of the T. cruzi genome and whose transcription is controversial in the literature. The transcription of the 195 SAT was evidenced by northern blot and RT-PCR experiments. Transcripts of 195 SAT were detected in polyA+ and poly- RNA fractions. These transcripts apparently do not contain the SL sequence, present in trypanosomatid mRNAs. By using oligonucleotides complementary to the two strands of 195 SAT, we concluded that both strands are transcribed. Although it is clear that 195 SAT is transcribed, its biological function remains unknown.

We describe conditions that improve the specificity of amplification of a G + C-rich (57% G + C) DNA by PCR. Under standard conditions a 368-bp segment of the approx. 2.1-kb repeat unit of a satellite DNA that accounts for approx. 3% of the genome of the Bermuda land crab, Gecarcinus lateralis, was not amplified specifically. To establish optimal conditions for amplification of the segment of the G + C-rich satellite, we used two genetically engineered enzymes, AmpliTaq DNA polymerase and AmpliTaq DNA polymerase. Stoffel fragment (SF), and a number of denaturants or co-solvents. In the absence of denaturants or co-solvents, amplified products of both enzymes contained non-specific bands upon gel electrophoresis. Addition of certain denaturants or co-solvents to PCR mixtures resulted in the production of the single specific band of the expected size. Reagents that improved specificity of the amplified product were formamide, glycerol, DMSO, Tween-20 and NP-40; on the other hand, urea, ethanol and 1-methyl-2-pyrrolidone (NMP) inhibited amplification. Of the two enzymes, SF was more specific and efficient. The products of AmpliTaq DNA polymerase included one or more extra bands, even in the presence of denaturants or co-solvents, except for glycerol or DMSO.

Genomes of higher plants comprise a large proportion of repetitive DNAs, where one major class is satellite DNA. Satellite DNA is organized in tandem arrays of basic repeating units, which often occurs in heterochromatin of centromeric/pericentromeric and intercalary as well as subtelomeric regions. Besides these typical satellite repeats, there are also non-typical satellite DNAs, which are organized in short tandem arrays and integrated into a transposable element. The chromosomal localization of non-typical satellites is not in large regions of heterochromatin, but tend to be dispersed along chromosomes. This thesis describes the identification of the major repeat classes including major satellite content in six beet and related species. The focus was on identification and characterization of new satellite families in the beet genomes. In this study, the information regarding repetitive DNA as well as satellite families fraction in six beet and related species was gained based on graph-based clustering of next generation sequenced short sequence reads. The repeat proportion of the six analyzed species ranges from 34.4% in C. quinoa to 65.6% in B. lomatogona, in which the portion of nearly 50% belongs to B. vulgaris, B. nana, P. procumbens, and P. patellaris. Among all classes of repetitive DNAs, LTR retrotransposons are the most abundant repeat type in all analyzed genomes, which is a common feature of higher plant genomes. The other repeat sequences are DNA transposons, rDNA, and satellite DNA with variable portions in different species. A set of satellite families in each species was analyzed in detail and reflects the relationship between six species. The closely related relationship between B. lomatogona and B. nana as well as between P. procumbens and P. patellaris is affirmed by seven and 13 satellite families shared between two species, respectively. Similarly, the closer relationship between B. vulgaris and two species B. lomatogona and B. nana than between B. vulgaris and two species P. procumbens and P. patellaris from the sister-genus Patellifolia is also confirmed. C. quinoa is a distantly related species and this is reflected by vastly different satellite content. Therefore, satellite DNA analysis might be a useful tool to trace species evolution. In the B. lomatogona genome, by the application of RepeatExplorer tool, six novel tandemly repeated DNA sequences were identified and designated BlSat1-BlSat6. The three typical satellite families BlSat1, BlSat5, and BlSat6 are organized in tandem arrays in large heterochromatic blocks. BlSat1 is mainly localized in the pericentric region of the chromosome 3, 5, 6, and 9, while BlSat5 is amplified in the pericentromeric region of the chromosome 3, 5, and 7. BlSat6 is a chromosome-specific satellite and is located in the subtelomeric region on the south arm of the chromosome 8. The other three satellite families BlSat2, BlSat3, and BlSat4 are characterized as non-typical satellite DNA because of their dispersed distribution along chromosomes. BlSat2 and BlSat3 are identified as a tandem repeat domain in Ogre/Tat retrotransposons. The occurrence of one or several short tandem arrays in a transposable element is a common phenomenon in both animals and plants. These short repeats are considered to be continuously evolving and eventually amplifying to new satellite families. Furthermore, the distribution of the six new satellite families in beet and related species was confirmed by comparative PCR, comparative Southern hybridization, and mapping of sequence reads from referent species against each satellite sequence. The BlSat1 and BlSat6 satellite families are specific for the genus Beta, while BlSat5 is only amplified in two sections Corollinae and Nanae of the genus Beta. BlSat4 is an ancient satellite family which exists in all tested species belonging to the genera Beta, Patellifolia, Chenopodium, and Spinacia, whereas BlSat2 and BlSat3 might have evolved before the separation of the genus Beta and Patellifolia but their sequences have been lost or heavily diverged during the species radiation. Comparison of two wild beet genomes P. procumbens and P. patellaris was performed aiming to address the open question whether P. patellaris is auto- or allotetraploid. The high similarity between these two genomes indicates their close relationship. However, the genetic difference between two genomes, in particular the molecular characteristics as well as the chromosomal localization of two satellite families PproSat1 and PpatSat1, might support a hypothesis that P. patellaris is allotetraploid species with a half of its chromosome set derived from P. procumbens. The results obtained in this work might provide comprehensive information of the repetitive classes as well as satellite families in the genomes of beets and related species. The results can be used as the species-specific and chromosome-specific markers in beet genome studies.

O presente trabalho teve como objetivos estimar os efeitos da inclusão de linha materna e do DNA mitocondrial (mtDNA) nos modelos de avaliação genética, avaliar o efeito do DNA satélite em características de desenvolvimento ponderal, bem como estimar parâmetros genéticos para características de carcaça e idade ao primeiro parto. Análises do mtDNA foram realizadas a partir de sangue coletado de 457 animais da raça Nelore, linhagem Lemgruber. Para as características de desenvolvimento ponderal foram analisados 14.432 registros de produção relacionados com 16.561 animais no pedigree. Para característica reprodutiva e as de carcaça foram analisados 4.621 registros de idade ao primeiro parto e 951 registros de área de olho de lombo e espessura de gordura subcutânea, relacionados com 7.135 registros de pedigree. As estimativas dos efeitos de linha materna e DNA mitocondrial foram analisadas sob 5 modelos diferentes. Os componentes de (co)variância para idade ao primeiro parto e características carcaça foram estimados em análises uni e bicaracterísticas. Não foram encontrados efeitos significativos de linhagem materna ou de DNA mitocondrial sobre as características de produção. Entretanto, foi verificado que animais com mitocôndria de origem Bos taurus, apresentam uma pequena superioridade na média de seus valores genéticos para as características de desenvolvimento ponderal. Animais com padrão de DNA satélite B tiveram suas médias ligeiramente superiores aos outros padrões. Os efeitos da precocidade de acabamento sobre a precocidade sexual devem ser avaliados utilizando-se estruturas de dados mais adequadas. / The aim of this research was to estimate the effect of the maternal lineage and mitochondrial DNA inclusion in the genetic evaluation model for growth traits, to evaluate the satellite DNA effect in these traits and to estimate genetic parameters for carcass traits and age at first calving. Blood samples were collected to mtDNA analysis from 457 Nellore cattle. Growth traits were analyzed from 14,432 production data and 16,561 pedigree data. Reproductive and carcass traits were analyzed from 4,621 age at first calving data and 951 loin-eye area and subcutaneous fat thickness data related to 7,135 pedigree data. Maternal lineage and mitochondrial DNA estimative were analyzed under 5 different models, (co)variance components for age at first calving and carcass traits were estimated in single and multiple-trait. There were no significant effects of maternal lineage or mitochondrial DNA related to production traits, but animals harboring Bos taurus mtDNA showed a low superiority on breeding values average for growth traits and animals harboring satellite DNA B pattern also showed a low superiority related to the other patterns. Further studies are suggested to evaluate the backfat thickness precocity effect related to sexual precocity.

Made available in DSpace on 2017-07-21T20:00:01Z (GMT). No. of bitstreams: 1 Patricia Barbosa.pdf: 1571215 bytes, checksum: daac7b661ca93cbfd05ca0e7cda85213 (MD5) Previous issue date: 2013-02-08 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The most part of the eukaryote genomes is constituted for repetitive DNA or multiple copies DNA, which has already been considered as “junk”, may be associated to the heterochromatin. In this study three Astyanax scabripinnis populations from Pindamonhangaba and Guaratinguetá (SP, Brazil) rivers and stream and one population from Maringá (PR, Brazil) were analyzed about the nucleolar organizing region (NORs), As51 satellite DNA, 18S and 5S rDNA location. Moreover, repetitive sequences were isolated and mapped through Cot-1 technique, which showed homology with UnaL2, a LINE type retrotransposon. The fluorescent in situ hybridization (FISH), with the isolated built retrotransposon probe, evidenced disperse labeled and stronger in centromeric and telomeric chromosomes regions, co-located and interspersed with the 18S DNAr and As51, proven by the fiber-FISH technique. The B chromosome of those populations showed very conspicuous labeled with the LINE probe, also co-located with the As51 sequences. The NORs were actives in a single site of a homologue pair in all three populations, with no evidence that the transposable elements and repetitive DNA have influence in its regulation at the performed analyzes level. / A maior parte do genoma dos eucariotos é constituída por DNA repetitivo ou DNA de múltiplas cópias, o qual já foi considerado “lixo”, podendo estar associado à heterocromatina. Neste estudo foram analisadas três populações de Astyanax scabripinnis provenientes de rios e córregos de Pindamonhangaba e Guaratinguetá (SP, Brasil) e uma população da cidade de Maringá (PR, Brasil) quanto a localização das regiões organizadoras de nucléolo (RONs), DNA satélite As51, DNA ribossomal (DNAr) 18S e DNAr 5S. Ainda, foram isoladas e mapeadas sequências repetitivas por meio da técnica de Cot-1, que mostrou homologia com UnaL2, retrotransposon do tipo LINE. A hibridação in situ fluorescente (FISH), com sonda construída para o retrotransposon isolado, evidenciou marcações dispersas e mais concentradas em regiões centroméricas e teloméricas dos cromossomos, co-localizadas e interespaçadas com DNAr 18S e As51, comprovada pela técnica de fiber-FISH. O cromossomo B das populações mostrou marcações bastante conspícuas com a sonda LINE, também co-localizada com sequências As51. As RONs apresentaram-se ativas em sítios únicos de um par homólogo nas três populações, não havendo indícios de que elementos transponíveis e DNA repetitivo tenham influência na sua regulação ao nível das análises realizadas.

Megasatellites are polymorphic tandem repetitive sequences with repeat-units longer than or equal to 1000 base pairs. The novel algorithm Megasatfinder predicts megasatellites in the human genome. A structured method of analysing the algorithm is developed and conducted. The analysis method consists of six test scenarios. Scripts are created, which execute the algorithm using various parameter settings. Three nucleotide sequences are applied; a real sequence extracted from the human genome and two random sequences, generated using different base probabilities. Usability and accuracy are investigated, providing the user with confidence in the algorithm and its output. The results indicate that Megasatfinder is an excellent tool for the detection of megasatellites and that the generated results are highly reliable. The results of the complete analysis suggest alterations in the default parameter settings, presented as user guidelines, and state that artificially generated sequences are not applicable as models for real DNA in computational simulations.

<p>Megasatellites are polymorphic tandem repetitive sequences with repeat-units longer than or equal to 1000 base pairs. The novel algorithm Megasatfinder predicts megasatellites in the human genome. A structured method of analysing the algorithm is developed and conducted. The analysis method consists of six test scenarios. Scripts are created, which execute the algorithm using various parameter settings. Three nucleotide sequences are applied; a real sequence extracted from the human genome and two random sequences, generated using different base probabilities. Usability and accuracy are investigated, providing the user with confidence in the algorithm and its output. The results indicate that Megasatfinder is an excellent tool for the detection of megasatellites and that the generated results are highly reliable. The results of the complete analysis suggest alterations in the default parameter settings, presented as user guidelines, and state that artificially generated sequences are not applicable as models for real DNA in computational simulations.</p>

Les génomes des espèces aviaires ont des caractéristiques particulières comme la structure des chromosomes et le contenu en séquences répétées. En effet, alors que dans les génomes vertébrés, la proportion de répétitions dans le génome varie de 30 à 55 %, dans les espèces aviaires, cette proportion est plus faible et varie de 8 à 10 %. L’annotation du contenu répété est le plus souvent réalisée avec le programme RepeatMasker qui s’appuie généralement sur la banque de séquences répétées Repbase. Ce genre de méthode repose uniquement sur la séquence des éléments transposables connus. De fait, ce programme n’est pas en mesure de détecter de nouvelles séquences répétées, et la qualité de l’annotation sera donc dépendante de la banque de séquences d’éléments transposables utilisée. De plus en plus d’études montrent que les éléments transposables jouent un rôle dans le fonctionnement du génome et peuvent influer sur l’expression des gènes. Il est donc primordial que l’annotation de ces séquences soit la plus complète possible. Au cours de ma thèse a été mise en place une stratégie d’annotation des séquences répétées que nous avons élaborée et appliquée à un génome de grande taille, celui de la poule rouge de jungle. L’annotation ainsi obtenue m’a permis d’étudier l’organisation du génome de cette espèce au travers de ses séquences répétées et éléments transposables. / The genomes of avian species have special features such as the structure of chromosomes or their content in repeated sequences. Indeed, compared to vertebrate genomes in which the amount of repetitions varies from 30 to 55%, it is lower in avian species and varies from 8 to 10%. The annotation of repeated content is most often done with the RepeatMasker program that is generally use the Repbase database of repeated sequences. This kind of approach is based solely on the sequence of already known transposable elements. In fact, this program is not able to detect new repeats and in consequence produced annotations with a quality that depends on the sequences of transposable elements used. More and more studies show that transposable elements play a role in the functioning of the genome and can influence gene expression. It is therefore essential that the annotation of these sequences is as complete as possible. There are many programs using methods for detecting de novo transposable elements, either by searching for characteristic structures, or by comparing the genome against itself. However, no standard strategy of annotation for repeated sequences have been defined yet. My thesis aims to set-up a standard strategy of annotation for repeated sequences that was applied to a large genome, that of the red jungle fowl. The obtained annotation allowed me studying the genome organization in this species through its repeated sequences and transposable elements.

Submitted by KETERYNE RODRIGUES DA SILVA null (kethy-rs@hotmail.com) on 2016-04-18T13:44:34Z No. of bitstreams: 1 Dissertação PRONTA pdf.pdf: 2073917 bytes, checksum: a152ff76ebcbbec56c3a78a1d420c107 (MD5) / Approved for entry into archive by Ana Paula Grisoto (grisotoana@reitoria.unesp.br) on 2016-04-18T19:53:38Z (GMT) No. of bitstreams: 1 silva_kr_me_rcla.pdf: 2073917 bytes, checksum: a152ff76ebcbbec56c3a78a1d420c107 (MD5) / Made available in DSpace on 2016-04-18T19:53:38Z (GMT). No. of bitstreams: 1 silva_kr_me_rcla.pdf: 2073917 bytes, checksum: a152ff76ebcbbec56c3a78a1d420c107 (MD5) Previous issue date: 2016-03-09 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O DNA pode estar organizado no genoma em sequências de cópias únicas e em sequências que se repetem várias vezes, denominadas sequências repetitivas. Estas sequências são constituídas basicamente por repetições em tandem (satélites, minissatélites e microssatélites) ou dispersas (transposons ou retrotransposons), e parecem estar envolvidas em diversos eventos celulares importantes, como por exemplo nos processos de replicação do DNA, de recombinação, de expressão gênica e de evolução dos cromossomos, auxiliando na manutenção e propagação do material genético celular. Em nível cromossômico parecem ser responsáveis por proporções significativas das variações cariotípicas observadas em diversos grupos. O gênero Ancistrus (Siluriformes, Loricariidae) apresenta atualmente 68 espécies nominais, e uma enorme quantidade de espécies ainda não identificadas. Alguns trabalhos vêm utilizando sequências repetitivas também em análises citogenéticas e moleculares para identificação de cromossomos homólogos e marcadores cariotípicos interessantes que podem auxiliar os trabalhos de identificação de espécies. Apesar de serem amplamente estudadas em diversos organismos, há ainda muito a ser entendido sobre essas sequências e sua organização no genoma. Este trabalho teve como objetivo isolar sequências repetitivas no genoma de espécies de Ancistrus, afim de encontrar possíveis marcadores para o gênero, que pudessem contribuir para o entendimento da taxonomia deste grupo, bem como auxiliar no entendimento do processo de evolução dos cromossomos sexuais dessas espécies. Dentre as sequências isoladas está um transposon da família TC1/mariner que se mostrou presente em todos os cromossomos das espécies analisadas e dois DNAs satélites que se apresentam acumulados em regiões centroméricas de alguns cromossomos. Sendo assim, este estudo resultou em dados que poderão contribuir com o entendimento da evolução cariotípica do gênero Ancistrus bem como fornecer mais informações sobre características e evolução dos cromossomos sexuais em peixes. / DNA may be organized in the genome as single copy sequences and as sequences that are repeated several times, called repetitive sequences. These sequences are basically constituted by tandem repeats (satellites, minisatellites and microsatellites) or scattered (transposons and retrotransposons), and seem to be involved in important cellular events such as, DNA replication process, recombination, gene expression and chromosome evolution, assisting in maintenance and spread the genetic material of cells. At chromosome level, seems to be significant proportions of karyotypic variations observed in several groups. The genus Ancistrus (Siluriformes, Loricariidae) has, actually, 68 nominal species and several species not identified yet. Repetitive sequences have been used in molecular cytogenetic analysis for identification of homologous chromosomes and interesting karyotypic markers that can assist in species identification works. Despite being widely studied in several organisms, there is still much to be understood about these sequences and their organization in the genome. This study aimed to isolate repetitive sequences in the genome of Ancistrusspecies in order to find possible markers for the genus, which could contribute to the understanding of the taxonomy of this group and to the understanding of the process of evolution of sex chromosomes of these species. Among the isolated sequences, there is a family of TC1/mariner transposon that showed to be present in all the chromosomes of the analyzed species, and two satellites DNAs that have accumulated in centromeric regions. Thus, this study resulted in data that could be contribute to understanding the karyotype evolution of Ancistrus genus as well as providing more information on the characteristics and evolution of sex chromosomes in fish.

O presente trabalho teve como objetivos estimar os efeitos da inclusão de linha materna e do DNA mitocondrial (mtDNA) nos modelos de avaliação genética, avaliar o efeito do DNA satélite em características de desenvolvimento ponderal, bem como estimar parâmetros genéticos para características de carcaça e idade ao primeiro parto. Análises do mtDNA foram realizadas a partir de sangue coletado de 457 animais da raça Nelore, linhagem Lemgruber. Para as características de desenvolvimento ponderal foram analisados 14.432 registros de produção relacionados com 16.561 animais no pedigree. Para característica reprodutiva e as de carcaça foram analisados 4.621 registros de idade ao primeiro parto e 951 registros de área de olho de lombo e espessura de gordura subcutânea, relacionados com 7.135 registros de pedigree. As estimativas dos efeitos de linha materna e DNA mitocondrial foram analisadas sob 5 modelos diferentes. Os componentes de (co)variância para idade ao primeiro parto e características carcaça foram estimados em análises uni e bicaracterísticas. Não foram encontrados efeitos significativos de linhagem materna ou de DNA mitocondrial sobre as características de produção. Entretanto, foi verificado que animais com mitocôndria de origem Bos taurus, apresentam uma pequena superioridade na média de seus valores genéticos para as características de desenvolvimento ponderal. Animais com padrão de DNA satélite B tiveram suas médias ligeiramente superiores aos outros padrões. Os efeitos da precocidade de acabamento sobre a precocidade sexual devem ser avaliados utilizando-se estruturas de dados mais adequadas. / The aim of this research was to estimate the effect of the maternal lineage and mitochondrial DNA inclusion in the genetic evaluation model for growth traits, to evaluate the satellite DNA effect in these traits and to estimate genetic parameters for carcass traits and age at first calving. Blood samples were collected to mtDNA analysis from 457 Nellore cattle. Growth traits were analyzed from 14,432 production data and 16,561 pedigree data. Reproductive and carcass traits were analyzed from 4,621 age at first calving data and 951 loin-eye area and subcutaneous fat thickness data related to 7,135 pedigree data. Maternal lineage and mitochondrial DNA estimative were analyzed under 5 different models, (co)variance components for age at first calving and carcass traits were estimated in single and multiple-trait. There were no significant effects of maternal lineage or mitochondrial DNA related to production traits, but animals harboring Bos taurus mtDNA showed a low superiority on breeding values average for growth traits and animals harboring satellite DNA B pattern also showed a low superiority related to the other patterns. Further studies are suggested to evaluate the backfat thickness precocity effect related to sexual precocity.