Thesis advisor: Frank Gollop / This dissertation analyzes advertising strategies in specialized markets like the prescription drugs, travel, health insurance and real-estate markets where the consumers' purchasing decisions are influenced by experts (e.g., doctors, travel agents, employers and real-estate agents). / Thesis (PhD) — Boston College, 2006. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: Economics.

The two time-honoured tasks of universities are teaching and research which have long provided society with specific skills and new knowledge and ideas. Expectations have increased exponentially and demands are originating from a much wider range of stakeholders. Universities are now given progressively more important roles in economic expansion, social development, better forms of political organization and governance, plus providing education for more students, and developing and transferring technology to industry. The capacity of universities to respond is insufficient, in both the developed and developing worlds. New models to guide the evolution of universities include the triple helix, the creation of entrepreneurial or specialized universities, large-scale excellence-driven environments or the concept of developmental universities. Most of these ultimately suggest that the universities move towards technology-oriented third missions, thus a closer interaction with enterprises. This special issue of Science and Public Policy explores such issues in 12 countries.

abstract: Over the last few decades, specialized courts have received an increasing amount of research attention. The existing literature mostly supports drug courts and demonstrates their effectiveness in reducing recidivism and substance abuse, more generally (Belenko, 1998; Bouffard & Richardson, 2007; Gottfredson, Najaka, & Kearley, 2003). Whether the drug court model “works” across offender subgroups remains an open empirical question. The current study uses data originally collected by Rossman and colleagues (2003-2009) for the Multi-Site Adult Drug Court Evaluation (MADCE) to examine the effect of drug court participation on recidivism among unique offender subgroups. First, a context-specific risk score is used to examine recidivism outcomes. Second, offender subgroups are statistically created using latent class analysis (LCA). Recidivism outcomes are then assessed by subgroup, with these results compared to the initial measure of risk. Both analyses are performed using the full sample of drug court participants and the comparison groups. Finally, the third model uses a split sample analysis by court participation to explore the full effects of drug court. The findings of the present study contribute to the theoretical literature and help inform future policy regarding risk assessment and the treatment of offenders in drug courts. / Dissertation/Thesis / Masters Thesis Criminology and Criminal Justice 2018

In Streptomyces bacteria, the expression of many antibiotic biosynthetic clusters is controlled by both cluster-specific regulators and more globally-acting regulators; however, much remains unknown about the factors that govern antibiotic production. In Streptomyces venezuelae, we have discovered that the broadly-conserved nucleoid-associated protein Lsr2, plays a major role in repressing specialized metabolic cluster gene expression. To understand how Lsr2 exerts its gene silencing effects, we focused our attention on the well-studied, but transcriptionally silent, chloramphenicol cluster in S. venezuelae. We established that Lsr2 represses transcription of the chloramphenicol cluster by binding DNA both within the cluster and at distal positions. CmlR is a known activator of the chloramphenicol cluster, but expression of its associated gene is not under Lsr2 control. We discovered that CmlR functions to ‘counter-silence’ Lsr2 activity, alleviating Lsr2 repression and permitting chloramphenicol production, by recruiting RNA polymerase. Lsr2 plays a central role in controlling antibiotic production in Streptomyces; however, beyond this counter-silencing activity, little is known about how Lsr2 is regulated. We identified regulators that could control the expression of lsr2, and found that Lsr2 and LsrL, an Lsr2 homologue that is encoded by all streptomycetes, interact directly with each other, and that their respective DNA-binding activities are altered by the presence of the other protein. These data suggest that LsrL may impact Lsr2 activity in regulating antibiotic production in Streptomyces. Beyond Lsr2, we wanted to develop a comprehensive understanding of the regulatory proteins that impact biosynthetic gene cluster expression. To define the regulatory protein occupancy of antibiotic clusters, we developed ‘in vivo protein occupancy display-high resolution’ (IPOD-HR) technology for use in Streptomyces. This work will lay the foundation for establishing a comprehensive regulatory network map for biosynthetic clusters in Streptomyces, and guide future work aimed at stimulating the expression of metabolic clusters in any Streptomyces species. / Thesis / Doctor of Philosophy (PhD) / Streptomyces bacteria produce the majority of naturally-derived antibiotics, and they have the genetic potential to produce many more antibiotics and antibiotic-like compounds (‘specialized metabolites’). Specialized metabolism is controlled by multiple regulatory systems. In Streptomyces venezuelae, we have discovered that the nucleoid-associated protein, Lsr2, represses the expression of most specialized metabolic clusters, and manipulating Lsr2 activity can stimulate antibiotic production. To better understand how Lsr2 exerts its repressive effect, we explored how Lsr2 controlled the production of a known antibiotic. We ultimately identified multiple regulators that could impact the expression and/or activity of Lsr2. Building on the regulatory foundation provided by Lsr2, we then set out to establish a comprehensive regulatory network that governs biosynthetic gene cluster expression. Collectively, this work improves our understanding of antibiotic gene regulation in Streptomyces bacteria, and has the potential to guide novel strategies aimed at stimulating the production of new antibiotics in Streptomyces.

Depuis plus de 80 ans, le métabolisme spécialisé nous fournit de nombreuses molécules utilisées en médecine, en particulier comme anti-infectieux. Aujourd’hui, avec l’augmentation mondiale de la résistance aux antimicrobiens, de nouveaux antibiotiques sont indispensables. Une des réponses à cette pénurie grave pourrait provenir de la biologie synthétique. Dans le domaine du métabolisme spécialisé, la biologie synthétique est utilisée en particulier pour la biosynthèse de métabolites non naturels. Parmi les métabolites spécialisés, les peptides non ribosomiques constituent une cible attrayante, car ils nous ont déjà fourni des molécules à haute valeur clinique (ex. les antibiotiques vancomycine et daptomycine). De plus, la plupart sont synthétisés par des enzymes multimodulaires appelées synthétases de peptides non ribosomiques (NRPS), et sont diversifiés davantage par des enzymes de décoration. Ainsi, ces voies de biosynthèse se prêtent particulièrement à la biosynthèse combinatoire, consistant à combiner des gènes de biosynthèse provenant de divers groupes de gènes ou, dans le cas des NRPS, à combiner des modules ou domaines pour créer de nouvelles enzymes. Cependant, si plusieurs études ont établi la faisabilité de telles approches, de nombreux obstacles subsistent avant que les approches combinatoires de biosynthèse soient totalement efficaces pour la synthèse de nouveaux métabolites. Les travaux présentés ici s’inscrivent dans le cadre d’un projet visant à comprendre les facteurs limitant les approches de biosynthèse combinatoire basées sur les NRPS, en utilisant une approche de biologie synthétique. Nous avons choisi de travailler avec les NRPS responsables de la biosynthèse des pyrrolamides. En effet, ces NRPS sont constitués uniquement de modules et de domaines autonomes, et donc particulièrement adaptés aux manipulations génétiques et biochimiques. La caractérisation du groupe de gènes de biosynthèse du pyrrolamide anthelvencine constitue la première partie de cette thèse et nous a fourni de nouveaux gènes pour notre étude. La deuxième partie a consisté à construire de vecteurs intégratifs modulaires, outils essentiels pour la construction et l’assemblage de cassettes génétiques. La dernière partie présente la reconstruction du groupe de gènes du pyrrolamide congocidine, basée sur la construction et l’assemblage de cassettes de gènes synthétiques. Dans l’ensemble, ces travaux ouvrent la voie à de futures expériences de biosynthèse combinatoire, expériences qui devraient contribuer à une meilleure compréhension du fonctionnement précis des NRPS. / For more than 80 years, specialized metabolism has provided us with many molecules used in medicine, especially as anti-infectives. Yet today, with the rise of antimicrobial resistance worldwide, new antibiotics are crucially needed. One of the answers to this serious shortage could arise from synthetic biology. In the field of specialized metabolism, synthetic biology is used in particular to biosynthesize unnatural metabolites. Among specialized metabolites, non-ribosomal peptides constitute an attractive target as they have already provided us with clinically valuable molecules (e.g. the vancomycin and daptomycin antibiotics). In addition, most are synthesized by multimodular enzymes called non-ribosomal peptide synthetases (NRPS) and further diversified by tailoring enzymes. Thus, such biosynthetic pathways are particularly amenable to combinatorial biosynthesis, which consists in combining biosynthetic genes coming from various gene clusters or, in the case of NRPSs, combining modules or domains to create a new enzyme. Yet, if several studies have established the feasibility of such approaches, many obstacles remain before combinatorial biosynthesis approaches are fully effective for the synthesis of new metabolites. The work presented here is part of a project aiming at understanding the limiting factors impeding NRPS-based combinatorial biosynthesis approaches, using a synthetic biology approach. We chose to work with the NRPSs involved in the biosynthesis of pyrrolamides. Indeed, these NRPS are solely constituted of stand-alone modules and domains, and thus, particularly amenable to genetic and biochemical manipulations. The characterization of the biosynthetic gene cluster of the pyrrolamide anthelvencin constitutes the first part of this thesis, and provided us with new genes for our study. The second part involved the construction of modular integrative vectors, essential tools for the construction and assembly of gene cassettes. The final part presents the successful refactoring of the congocidine pyrrolamide gene cluster, based on the construction and assembly of synthetic gene cassettes. Altogether, this work paves the way for future combinatorial biosynthesis experiments that should help deciphering the detailed functioning of NRPSs.

Orientador: Diva Cardoso de Camargo / Banca: Francis Henrik Aubert / Banca: Ieda Maria Alves / Banca: Claudia Maria Xatara / Banca: Eli Nazareth Bechara / Resumo: Esta pesquisa visa realizar um estudo a respeito dos termos, colocações e colocações especializadas estendidas presentes em contratos sociais e estatutos sociais que representam os corpora de pesquisa. Nesta pesquisa, também observaremos as semelhanças e diferenças nos corpora de traduções jurídicas e juramentadas, no que concerne ao uso desses termos e padrões lexicais, assim como apontaremos aqueles que são mais frequentemente empregados em documentos do tipo contrato social e estatuto social. A investigação baseia-se na abordagem interdisciplinar dos Estudos da Tradução Baseados em Corpus, da Linguística de Corpus, da Fraseologia, de modo mais específico das colocações, das colocações especializadas e das unidades fraseológicas especializadas. A Terminologia, por meio de seus pressupostos teóricos, também traz sua contribuição para a pesquisa, assim como os trabalhos sobre a tradução juramentada. Uma das motivações que delineia este estudo reside no fato de a tradução juramentada ser considerada de grande relevância nas relações comerciais, sociais e jurídicas entre as nações. Para realizar este estudo, compilamos um corpus de estudo (CE1) constituído por contratos sociais e estatutos sociais traduzidos no modo juramentado, nas direções tradutórias inglês português e português inglês, extraídos de Livros de Registro de Traduções, pertencentes a tradutores juramentados credenciados pela Junta Comercial de dois Estados brasileiros; e um corpus de estudo (CE2) formado por documentos de mesma natureza traduzidos sem o processo de juramentação, nas mesmas direções tradutórias. Além destes corpora, construímos dois corpora comparáveis, formados pelos referidos documentos originalmente escritos em português e em inglês. Os resultados desta pesquisa mostraram várias semelhanças, no tocante aos termos empregados em documentos traduzidos... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: This investigation aims at carrying out a study on terms, collocations and extended specialized collocations present in articles of incorporation/articles of organization/articles of association and bylaws that represent our research corpora. We will also observe similarities and differences in sworn and legal translation corpora, which concerns the use of such terms and lexical patterns, as well as point out the ones which are more frequently used in the focused documents. This research derives its theoretical and methodological sources from Corpus-Based Translation Studies, Corpus Linguistics, Phraseology, more specifically from collocations, specialized collocations and specialized phraseological units (SPUs). Terminology, from its theoretical standpoint, also offers its contribution to this study, as well as essays on sworn translation. One of the aspects that motivates this study is the fact that sworn translation is considered to be of great relevance to commercial, social and legal relations among nations. To conduct this research, we compiled a study corpus (CE1) composed of articles of incorporation/articles of organization/articles of association and bylaws submitted to the process of sworn translation in the English Portuguese and Portuguese English directions, excerpted from the Books of Sworn Translation Records, made available by five Brazilian sworn translators, duly sworn by the Board of Trade of two Brazilian States; a study corpus (CE2) made up of documents of the same nature not submitted to the process of sworn translation, in the same translation directions. Besides these corpora, we also built two comparable corpora formed by the referred documents originally written in Portuguese and in English. The results obtained in this research showed some similarities which refer to the terms used in documents submitted to the process of sworn translation... (Complete abstract click electronic access below) / Doutor

The purpose of this study is to describe and analyse how elite sport students who studied at the National Sport Upper Secondary Schools with specialization on table tennis, experienced the aim and content of the subject Physical Education related to the learning of the subject, learning of the Specialized Physical Education, as well as their own elite investment. The theoretical perspective used in the study is Lave and Wengers (1991) theoretical concept the Community of practice. Where the focus is based on how the students like a community fulfills the criteria who used to characterize the Community of practice and also the aspect of "learning as participate" in the Community of practice. The results showed that the students did not experience a specific aim with the subject Physical Education. The biggest difference was in how the students experienced the learning of the subject Specialized Physical Education towards Physical Education. All of the students exemplify the meaning of studying at the National Sport Upper Secondary Schools with the aim of becoming an elite athlete. Therefore the subject Physical Education was seen by the students more as a subject of amusing character than a subject with knowledge, unlike what they experienced with the subject Specialized Physical Education. In relation to their own elite investment it became obvious that the students considered that they could not use the practical skills which were supposed to be developed in the subject of Physical Education. However two of the students emphasized that the theoretical knowledge was important elements in relation to their elite investment.

Background: The amount of time that it takes to get a breast cancer diagnosis is very important to patients. The Ontario diagnostic assessment unit (DAU) is designed to improve the quality and timeliness of care during a breast cancer diagnosis. This study described and examined the association between the length of the diagnostic interval and DAU use in Ontario, Canada. Methods: This was a retrospective cohort study among all breast cancer patients diagnosed between Jan 1st, 2011 and Dec 31st, 2011 in Ontario, Canada. DAU use and diagnostic intervals were described. The association between DAU use and the diagnostic interval was examined separately in a cohort of 2499 screen-detected patients and a cohort of 4381 symptomatic patients. Study data sources included administrative databases available at the Institute for Clinical Evaluative Sciences (ICES) and Cancer Care Ontario (CCO). The diagnostic interval was defined as the time from the index contact to the cancer diagnosis. DAU use was determined based on the payment record within the organized screening program as well as the hospital where patients were diagnosed. Multivariate median regressions were used to control for possible confounders. Results: On average, Ontario breast cancer patients waited 4.6 weeks to be diagnosed. Forty-eight percent were diagnosed in a DAU and 52% were diagnosed in the usual care route. In screen-detected patients, DAUs had a higher rate in meeting national timeliness targets compared to usual care (79.1% vs. 70.2%, p<0.001). DAU use was significantly associated with an 8.3-day decrease in the time to diagnosis (95% CI: 6.5-10.2) after controlling for potential confounders. In symptomatic patients, DAUs also had a higher rate in achieving the Canadian timeliness targets compare to usual care (71.7% vs. 58.1%, p<0.001). DAUs significantly reduced the time to diagnosis by 10 days (95% CI: 7.8-11.9) after controlling for possible confounders. Conclusions: We observed considerable variation in breast cancer diagnostic intervals and DAU use in Ontario. Use of Ontario DAUs was associated with improved diagnostic timeliness for breast cancer patients. / Thesis (Master, Community Health & Epidemiology) -- Queen's University, 2013-11-18 13:19:03.231

Leafflower plant/leafflower moth brood pollination mutualisms are widespread in the Paleotropics. Leafflower moths pollinate leafflower plants, but their larvae consume a subset of the hosts' seeds. These interactions are highly phylogenetically constrained: six clades of leafflower plants are each associated with a unique clade of leafflower moths (Epicephala). Here, we report a previously unrecognized basal seventh pollinating Epicephala lineageassociated with the highly derived leafflower clade Glochidionin Asia. Epicephala lanceolaria is a pollinator and seed predator of Glochidion lanceolarium. Phylogenetic inference indicates that the ancestor of E. lanceolaria most likely shifted onto the ancestor of G. lanceolarium and displaced the ancestral allospecific Epicephala pollinator in at least some host populations. The unusual and apparently coadapted aspects of the G. lanceolarium/E. lanceolaria reproductive cycles suggest that plant-pollinator coevolution may have played a role in this displacement and provide insights into the dynamics of host shifts and trait coevolution in this specialized mutualism.

This dissertation examines recidivism for domestic violence offenders under both traditional probation and specialized probation conditions. This research compares recidivism at a traditional probation locality (Riverside Criminal Justice Services) and a specialized domestic violence unit (Chesterfield Community Corrections Services) in order to determine the nature of recidivism at both localities. In addition, the research aims to identify individual risk factors which are statistically associated with recidivism. The research is based on secondary data and draws on offender criminal records generated by the Virginia State Police, as well the extraction of information from probation files at both localities. The multivariate models indicate that a higher degree of recidivism occurs at the specialized domestic violence unit, and that the experimental locality, along with a younger age, are consistently statistically significant predictors for recidivism. Less consistent but still statistically significant risk factors included positive drug tests, higher supervision levels, and unsuccessful probation completion. The implications of these findings, as well as policy recommendations and directions for future research are fully discussed.