The early life history dynamics of stocked, fingerling striped bass (Morone saxatilis) were evaluated in Smith Mountain Lake, Virginia, from 1994-96 and incorporated into an individual-based model to assess various stocking strategies in terms of their largest percent increases in first-year growth, overwinter survival, and recruitment to age 1. Age-0 striped bass exhibited dissimilar patterns of dispersion and size-dependent spatial distribution as a result of differences in habitat availability between stocking sites and water temperature preferences. Although size increased over the growing season, a bimodal length distribution developed by fall during both 1994 and 1995; this distribution consisted of large-mode juveniles (> 180 mm TL) that weighed several times more than small-mode fish (<140 mm TL). Differential growth was attributed primarily to size-dependent differences in food habits and diet quality: small-mode striped bass maintained a mixed, low quality diet of invertebrates and small, age-0 cyprinids, while large-mode juveniles consumed only larger, energetically more profitable age-0 alewives. This disparity in food habits, largely due to the inability of small-mode striped bass to consume distributionally- and morphologically-invulnerable age-0 alewives, resulted in size-dependent differences in physiological well-being as large-mode juveniles had amassed greater absolute energy stores than small-mode fish by the end of the growing season. Spring sampling revealed that the bimodal length distribution had become unimodal and was comprised almost entirely of large-mode juveniles. Because the few surviving small-mode striped bass collected during spring were extremely emaciated, it appears that this size group exhausted their energy stores and, consequently, starved over the winter. Individual-based model simulation results indicated that stocking juvenile striped bass at a median total length of 52 mm on 08 June, while maintaining the current stocking density at 300,000 fingerlings, would result in the largest percent increases in first-year growth, survival, and number of age-1 recruits. This strategy was also less sensitive to perturbations in alewife population parameters and water temperature regime, and was more robust to these variations than the existing stocking scheme (300,000 fingerling striped bass with a median total length = 42 mm introduced on 15 June). / Ph. D.

Smith Mountain Lake, Virginia supports a successful put-grow-take striped bass fishery. Empiric analysis of striped bass stocking has shown an inverse relationship between number of fingerling striped bass stocked and survival to age 1. Potential causes for this inverse relationship include largemouth bass predation on fingerling striped bass and mortality resulting from stocking stress. Cage studies performed in 1994 and 1995 quantified percentage of fingerlings lost due to hauling/handling stress. Mean mortalities ranged from 1.78% for Phase I fingerlings in 1994 to 99.5% for Phase II fingerlings (reared in a recirculating aquaculture system for increased size at stocking) in 1994. Mortality rates varied greatly and were probably directly related to length of transport and inadequate thermal tempering prior to stocking. Highest mortality occurred at transport times in excess of six hours and when receiving water was 5° C warmer than transport water. A trial in which Phase I fingerlings were caged without transport or temperature change resulted in no mortality. Predation mortality by largemouth bass was also considered as a source of poor first-year survival of striped bass in Penhook and Waterwheel stocking coves at Smith Mountain Lake. It was necessary to estimate largemouth bass population size, diet composition, and daily consumption (bioenergetic modeling) to determine the total number of striped bass lost to predation. Diet analysis revealed that age-0 striped bass made up a maximum of 2.5% of largemouth bass diets in the month following stocking; adult alewives constituted more than 60% by weight. The estimated number of striped bass lost was only 360 (0.1%) in 1994 and 3062 (1.2%) in 1995. Bioenergetic simulations demonstrated that predation could become significant in the unlikely event that the contribution of striped bass to largemouth diets increased to 10% or more. Based on results from diet analysis and a prey preference laboratory study, alewives appear to buffer predation of age-0 striped bass during the month after stocking. In 1994 and 1995, neither stocking stress associated with the typical Phase I fingerling stocking procedure nor largemouth bass predation resulted in substantial mortality of stocked fingerling striped bass. / Master of Science

Eight identical and independent pilot scale recirculating aquaculture production systems were populated with fingerling hybrid striped bass <i>(Morone chrysops</i> female x <i>Morone saxatilis male</i>). Three population densities were established with two replicates at 132 fishlm3 and three replicates each at 66 and 33 fishlm3. Water chemistry and water quality characteristics were monitored throughout the 228 day growth trial for all eight systems. A system component performance analysis was done for both the multi-tube solids clarifier and rotating biological contactor (RBC). Water chemistry and water quality analysis included dissolved oxygen (DO), alkalinity, ions, carbonaceous biochemical oxygen demand (CBODS), chemical oxygen demand (COD), dissolved organic carbon (DOC), total suspended solids (TSS), volatile suspended solids (VSS), total ammonia nitrogen (TAN) , nitrite and nitrate. The major ions present were chloride, nitrate, sulfate, phosphate, sodium, calcium, magnesium and potassium. Trace levels of TAN, nitrite, iron and copper were also observed. Sodium, calcium and chloride levels were controlled based on a preplaned water exchange and chemical management method. TAN, nitrite and nitrate levels increased over time with increasing feed rates but never reached levels toxic to the fish population. CBODS,COD, DOC, TSS and VSS increased over time increasing as a function of increasing feed rates. No correlation was observed between fish mortality or fish growth rates for the range of organic and solids parameters observed during this study. On average 67% of the TSS present was between 1.5 and 30 microns in size. Diurnal cycles were observed for DO, TAN and alkalinity. The magnitude of these cycles were population dependent. Multi-tube clarifiers removed an average 56% of all suspended solids in a single pass with 81 % removal efficiency for particles above 70 microns in size. Analysis of the system effluent generated by the clarifier indicates a high degree of similarity between the aquaculture effluent and standard municipal waste on a nutrient basis but with much higher levels of nitrogen and phosphate species. RBC nitrification performance was fitted to an empirical equation. A nearly constant TAN removal rate was observed over the range of mass loading experienced in this growth trial. This implies that within a reasonable range higher flow rates resulting in higher mass loading will yield higher TAN removal rates for a given RBC. / Master of Science

Molecular genetic markers can be used to assess genetic diversity, assign parentage, quantify inbreeding, and demonstrate structuring of populations across a system. Striped Bass Morone saxatilis, and Walleye Sander vitreus, are widely sought gamefishes in the Commonwealth of Virginia. Striped Bass along the Atlantic Coast and within the Roanoke River drainage exhibit low genetic variation. Screening 12 microsatellite DNA markers across the range to define population genetic structure, I found that anadromous populations in the Southeast and the Chesapeake Bay were differentiated from landlocked populations in the Roanoke River basin, with an average FST of 0.066. Range-wide, Striped Bass are differentiated between the landlocked and anadromous populations, which need to be managed separately. Within stocked populations in the Roanoke River basin, there have been impacts stemming from small numbers of broodstock propagated, and inter-individual relatedness is ~20% within stocked reservoirs. Walleye across the eastern native range were screened to better understand evolutionary history and to identify native marker alleles for the upper New River population. Population genetic variation at eight microsatellite loci showed differentiated stocks in Alabama, Mississippi River, Eastern Highlands (Tennessee, New, and Ohio Rivers), and the Great Lakes drainages. All estimates of effective numbers of breeding individuals were under 25, and all populations within all watersheds had ~15-20% inter-individual relatedness, likely effects of both natural demographic processes and stocking. The extent of Eastern Highlands Walleye includes both the Ohio and Tennessee basins. Although I did not identify new marker alleles for native New River Walleye, I determined that marker-assisted selection has increased the frequencies of existing marker alleles for the native stock. Application of the results of this project will contribute to better fisheries management for both of these important species. / Master of Science / Population genetics have proven useful for defining the most appropriate units for conservational management across a variety of species. Molecular genetic markers can be used to assess genetic diversity, assign parentage, quantify inbreeding, and demonstrate structuring of populations across a system. Striped Bass Morone saxatilis and Walleye Sander vitreus are both widely sought gamefishes in the Commonwealth of Virginia. I applied population genetic approaches to recognize genetically distinct groups of populations and to recommend genetically cognizant management practices. Striped Bass across the Atlantic Coast and in the Roanoke River drainage exhibit low genetic variation. After screening variation at 12 DNA markers, I found that Striped Bass are differentiated between landlocked and migratory populations, which need to be managed separately. Within stocked populations in the Roanoke River basin, there have been impacts stemming from propagation of small numbers of broodstock, and propagation and stocking practices will need to be changed to reduce apparent inbreeding depression. Walleye populations across the eastern native range were screened to better understand evolutionary history and to seek new marker alleles for the native upper New River population. After screening genetic variation at eight DNA marker loci, I identified four evolutionarily distinct stocks of Walleye across eastern North America. Although I did not identify new marker alleles for native upper New River native Walleye, I showed that marker-assisted selection has increased the frequencies of existing marker alleles over the past twenty years. The results of this project can contribute to better fishery management strategies for both of these important gamefish species.

The Chesapeake Bay is the largest estuary in the United States and is highly productive making it a key habitat for species like striped bass and summer flounder. Striped bass and summer flounder have exhibited changes in abundance over recent years within the Chesapeake Bay. There is a lack of understanding of how environmental factors may be impacting striped bass and summer flounder abundance and how other species may be responding to these environmental drivers. To improve our understanding of striped bass, summer flounder and the aquatic community we set out to identify the environmental drives of their abundance changes from local to global scales. The fish community in the Bay has previously shifted due to environmental perturbations, so we also investigate if the community has changed more recently. We used a hurdle model to standardize interannual abundance of fish species caught in the Chesapeake Bay from two sources of fishery-independent trawl survey data. This standardization process led to relative abundance indices for 58 late juvenile to adult species from 2002-2018, and relative abundance indices for 26 juvenile species from 1995-2019. Species with similar and contrary abundance trends with striped bass and summer flounder were identified through a correlation analysis, and life history traits were assessed between species to determine mechanisms of change. We then used nonmetric multidimensional scaling (nMDS) to see if the community structure had drastically changed, the applied a principal response curve (PRC) to investigate the spatial and functional group change of the community. Among the factors examined, sea surface temperature (SST) in the Bay has increased since 2006 based on change point analysis while the mean Bay SST range and gradient have both decreased. Striped bass have had variable abundance in the Bay but exhibited a positive correlation with increasing SST. Summer flounder have declined in abundance since 2006 in the Bay and exhibited a negative correlation with increasing SST. Striped bass and summer flounder had relationships with global climate oscillations such as the winter North Atlantic Oscillation, Atlantic Multidecadal Oscillation, and the Gulf Stream North Wall oscillation. From a community perspective, our results showed that the late juvenile to adult fish community shifted after 2011, which coincided with the freshwater surge due Hurricane Irene and Tropical Storm Lee. The increase in abundance of striped bass, Bay anchovy, and Atlantic menhaden, and decrease in abundance of summer flounder, weakfish, spot, and Atlantic croaker were the main species that contributed to the difference in community structure after 2011. The change in functional group structure after 2011 was defined by a decline in opportunistic demersal fish, and this change was significantly different in the portion of the Bay north of the Potomac River. We did not identify any significant correlation between functional groups and environmental factors but did discover the important relationship that top predators like striped bass and summer flounder have with their key fish prey species, both in presence and population trends. Long term monitoring and further research in to how the community changed over smaller periods and the distribution changes of species could improve our understanding of what is impacting the Chesapeake Bay community to inform better management strategies. / Master of Science / The Chesapeake Bay is an important habitat for many animals, including fish. Of the fish species that inhabit the Bay, striped bass and summer flounder are two highly sought after commercial and recreational species, and are top predators, which means they are economically and ecologically important. Striped bass and summer flounder have exhibited changes in abundance over recent years within the Chesapeake Bay, and there is a lack of understanding what environmental factors may be driving their abundance trends and if other species are responding similarly. To conserve striped bass and summer flounder, management strategies should consider the relationship these species have with their environment and other species. Through this research, we set out to understand what environmental factors are impacting their population trends, and the trends of the aquatic community which can be used to inform effective management decisions for the future. To accomplish our research goals, we first used a hurdle model to determine the interannual abundance of 58 adult and 26 juvenile fish species from 2002-2018 and 1995-2018 respectively. We then investigated whether any environmental factors exhibited relationships with striped bass and summer flounder. Again, through correlation analysis, we identified species with similar and contrary abundance trends with striped bass and summer flounder and compared the traits of these species to try to identify the mechanisms of their abundance trends. To visualize if and how much the fish community composition had changed over time we used nonmetric multidimensional scaling (nMDS), then applied a principal response curve (PRC) to investigate the spatial change and life history trait change in the fish community. The results showed us that striped bass and summer flounder do exhibit relationships with environmental factors. Sea surface temperature (SST) in the Bay has increased, while the range and gradient has decreased, and an increase in the mean SST occurred in 2006. We found that striped bass had variable abundance in the Bay but correlated positively with increasing SST, however summer flounder abundance has decreased since 2006 in the Bay and has a negative correlation with SST. Striped bass and summer flounder exhibited relationships with global climate-oscillations that impact the Chesapeake Bay, making those factors important to consider for their management. The results of the community assessment showed that the late juvenile to adult fish community shifted after 2011. This change in community structure coincided with a decrease in water quality and a freshwater surge in 2011 that was caused by Hurricane Irene and Tropical Storm Lee. After the community shift, striped bass, Bay anchovy, and Atlantic menhaden increased in abundance, while summer flounder, weakfish, spot and Atlantic croaker decreased in abundance. These species were the top species that contributed to the change in community structure. The change in community structure was greatest, and significant in the northern portion of the Bay. From these results we identified the important relationship between predators and prey both in species presence and abundance trends. Striped bass abundance increased alongside an increase of their key prey species, Bay Anchovy and Atlantic menhaden, while summer flounder abundance decreased along with two of their key prey species spot and Atlantic croaker. This research identified environmental factors that contribute to abundance trends of striped bass and summer flounder and highlighted the importance of multispecies interactions within the aquatic Chesapeake Bay community. Long term monitoring and more research into finer scale spatial and temporal changes of fish in the Bay could further improve management recommendations.

As aquaculture continues to contribute high quality protein to a greater proportion of the worlds growing population, fish producers have been pressured to increase overall production. However, associated with elevated production is greater stress due to crowding, reduced water quality, and other factors. These stressors impact the health and welfare of the farmed animal which has become of increasing concern to a more environmentally aware and health conscious consumer. New strategies must therefore be developed and adopted by the aquaculture industry to counteract negative consumer perceptions of industrial fish production while also stabilizing the industry. Better nutrition may enhance disease resistance of farmed fish, while fillet accumulations of specific health-related nutrients may simultaneously add value to the final product. This thesis summarizes research undertaken in an effort to enhance the nutritional value of fish by increasing fillet levels of selenium (Se). In addition, various biomarkers of fish health (lysozyme, ceruloplasmin and glutathione peroxidase (GSH-Px) activities), were examined to determine whether dietary Se supplementation had a positive impact upon fish immunocompetence. Moreover, the effect of vaccination was also examined using lysozyme and growth as indicators of fish performance. Hybrid striped bass (HSB), the fourth most valuable farmed fish and fifth in tonnage produced in the United States, were employed as a model animal. Se, an essential component of the antioxidant enzyme, glutathione peroxidase with many established health benefits was supplemented to HSB diets at various concentrations but was found to be without effect upon serum immune proteins or GSH-Px activity. This finding likely reflected the use of fishmeal within the dietary formulation, which possessed relatively high Se levels, together with sufficient storage of tissue Se within the experimental animals. Nevertheless, these studies determined that organic sources of Se were more efficiently accumulated in HSB muscle than traditional inorganic sources. A linear response occurred up to the highest dose used (3.2 mg kg⁻¹) over a 6 week study. Fillet Se accumulation (r²=0.95) proved to be a better indicator than the liver (r²=0.87).Se enhanced fish therefor appear to offer a route of entry for fish producers into the lucrative designer food market - especially since many hundreds of millions of people worldwide are believed to be Se-deficient. Studies undertaken with Se-deficient HSB confirmed findings from the aforementioned research and also indicate that Se-enhanced fillets might be produced using a finishing feed containing 1.5 mg Se kg⁻¹ 6-8 weeks prior to harvest. Accumulation of Se using this strategy resulted in a 100g portion of HSB fillets containing between 33-109 µg Se, amounting to a dietary intake of between 25-80 µg Se; a level that would satisfy present daily intake recommendations. Vaccination of HSB with a Streptococcus iniae oil-in-water vaccine was examined for its potential negative impacts upon HSB production performance. Vaccinated fish did not exhibit any significant reductions in growth but microarray studies revealed that together with many hundreds of genes, four immune-related genes were impacted by this procedure. This thesis discusses the results obtained with regard to their practical implications to the industry and welfare of cultured fish. / Master of Science

Florida's estuaries support a wide range of species yet little is known about tracemetal cycling among members of this important ecosystem. To examine the flow of trace metals through the Tampa Bay estuary, four fish species representing different trophic levels were analyzed for copper (Cu), zinc (Zn) and stable isotopes of carbon (C) and nitrogen (N). Species selected were the striped mullet (Mugil cephalus), tidewater mojarra (Eucinostomus harengulus), bay anchovy (Anchoa mitchilli), and sand seatrout (Cynoscion arenarius). Juvenile fish were collected from the Alafia, Hillsborough, Palm, and Little Manatee Rivers. Adults were collected from Tampa Bay. Combinations of trace metal and stable isotope analyses were used to evaluate geographic variability in trace metal concentrations among locations in Tampa Bay and to shed light on trophic pathways that lead to trace metal accumulation. In juvenile mullet, significant trends were found between Zn concentrations, stable isotope ratios, and standard length. Animals of the smallest size classes carry greater concentrations of zinc in their tissues and have distinct stable isotope ratios that reflect their recent life history as offshore planktivorous larvae. Interestingly, the ratio of Zn:Cu concentrations was highly conserved. While species-specific differences were observed, relatively small Zn:Cu variations suggest a possible bioregulatory mechanism that maintains an optimal Zn:Cu ratio even in the presence of elevated absolute metal concentrations. Stable isotope ratios proved to be an effective tracer of ontogenetic changes in fish diet and habitat. Carbon and nitrogen stable isotope analyses revealed that trophic relations between species are established very early in an organism's life history. The bay anchovy, a major prey item of the sand seatrout, has δ15N values very similar to this predator. Although trophic linkages between trace metals and stable isotopes proved difficult to interpret, the relation between zinc concentrations and δ¹³C values suggested that trace metal concentrations are highest in animals that utilize food webs based on terrestrial carbon.

Thesis (PhD)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: Within southern Africa, the widely distributed four-striped mouse Rhabdomys is parasitized by, amongst others, the host-specific ectoparasitic sucking louse, Polyplax arvicanthis. The present study investigated this parasite-host association from a phylogenetic and phylogeographic perspective utilizing mitochondrial and nuclear DNA markers. The findings support the existence of four species within Rhabdomys (three distinct lineages within the previously recognized arid-adapted R. pumilio and the mesic-adapted R. dilectus). These species have distinct geographic distributions across vegetational biomes with two documented areas of sympatry at biome boundaries. Ecological niche modelling supports a strong correlation between regional biomes and the distribution of distinct evolutionary lineages of Rhabdomys. A Bayesian relaxed molecular clock suggests that cladogenesis within the genus coincides with paleoclimatic changes (and the establishment of the biomes) at the Miocene-Pliocene boundary. Strong evidence was also found that the sucking louse P. arvicanthis consists of two genetically divergent lineages, which probably represent distinct species. The two lineages have sympatric distributions throughout most of the sampled range across the various host species and also occasionally occur sympatrically on the same host individual. Further, the absence of clear morphological differences among these parasitic lineages suggests cryptic speciation. Limited phylogeographic congruence was observed among the two P. arvicanthis lineages and the various Rhabdomys species and co-phylogenetic analyses indicated limited co-divergence with several episodes of host-switching, despite the documented host-specificity and several other traits predicted to favour congruence and co-divergence. Also, despite the comparatively smaller effective population sizes and elevated mutational rates found for P. arvicanthis, spatial genetic structure was not more pronounced in the parasite lineages compared to the hosts. These findings may be partly attributed to high vagility and social behaviour of Rhabdomys, which probably promoted parasite dispersal among hosts through frequent inter-host contact. Further, the complex biogeographic history of Rhabdomys, which involved cyclic range contractions and expansions, may have facilitated parasite divergence during periods of host allopatry, and host-switching during periods of host sympatry. Intermittent contact among Rhabdomys lineages could also have prevented adaptation of P. arvicanthis to specific host lineages, thus explaining the lack of host-specificity observed in areas of host sympatry. It is thus evident that the association between Polyplax arvicanthis and Rhabdomys has been shaped by the synergistic effects of parasite traits, biogeography, and host-related factors over evolutionary time. / AFRIKAANSE OPSOMMING: Binne suidelike-Afrika word die wyd-verspreide gestreepte veldmuis, Rhabdomys, onder andere deur die gasheer-spesifieke ektoparasitiese luis, Polyplax arvicanthis, geparasitiseer. Die huidige studie het hierdie parasiet-gasheer interaksie vanuit ‘n filogenetiese en filogeografiese oogpunt ondersoek deur van beide mitokondriale en nukluêre merkers gebruik te maak. Die bevindinge dui op die bestaan van vier spesies binne Rhabdomys, waaronder drie nuwe genetiese groepe binne die voorheen erkende R. pumilio asook R. dilectus. Hierdie spesies het nie-oorvleulende geografiese verspreidings binne spesefieke plantegroei biome met twee geidentifiseerde areas van simpatriese voorkoms by bioom grense. Ekologiese nis modellering ondersteun ‘n sterk korrelasie tussen biome en die verspreiding van die evolusionêre groepe binne Rhabdomys. ‘n Bayesiaanse verslapte molekulêre klok dui daarop dat kladoginese binne die genus gedurende paleoklimatiese veranderinge, wat tot die totstandkoming van die huidige biome gelei het, by die Mioseen-Plioseen grens plaasgevind het. Sterk bewyse is ook gevind dat die parasitiese luis P. arvicanthis uit twee geneties verskillende groepe, wat heel moontlik afsonderlike spesies verteenwoordig, bestaan. Hierdie genetiese groepe het simpatriese verspreidings oor meeste van die gebestudeerde geografiese area op die verskeie gasheer spesies en mag ook soms simpatries op dieselfde gasheer individu voorkom. Verder dui die afwesigheid van duidelike morfologiese verskille tusssen die parasiet genetiese groepe op moontlike kriptiese spesiasie. Beperkte filogeografiese ooreenstemming is tussen die P. arvicanthis genetiese groepe en die Rhabdomys spesies waargeneem en die vergelykende-filogenetiese analises het aangedui dat daar beperkte gesementlike-divergensie plaasgevind het met verskeie episodes van gasheer-wisseling, ten spyte van die gasheer-spesifieke aard van die parasiete asook verskeie ander kenmerke wat veronderstel is om filogeografiese ooreenstemming en gesementlike-divergensie te bevorder. Ten spyte van die vergelykbaar kleiner effektiewe bevolking groottes en verhoogde mutasie tempo wat vir P. arvicanthis gevind is, is die geografiese genetiese struktuur nie meer gedifferensieёrd in die parasiet groepe as in die gasheer nie. Hierdie bevindinge mag deels verklaar word deur die hoё beweeglikheid asook die sosiale gedrag van Rhabdomys, wat waarskynlik parasiet beweging tussen gashere bevorder deur gereelde tussen-gasheer kontak. Die komplekse biogeografiese geskiedenis van Rhabdomys, wat sikliese inkrimping en uitsetting van die geografiese verspreiding behels het, het heel moontlik parasiet divergensie tydens tydperke van gasheer allopatrie asook gasheer-wisseling tydens tydperke van gasheer simpatrie, gefasiliteer. Tussentydse kontak tussen Rhabdomys genetiese groepe kon aanpassing van P. arvicanthis tot sekere gasheer genetiese groepe verhoed het en verklaar dus die afwesigheid van waargenome gasheer-spesifisiteit in areas van gasheer simpatrie. Dit is dus duidelik dat die assosiasie tussen P. arvicanthis en Rhabdomys deur die sinergistiese uitwerking van parasiet kenmerke, biogeografie, asook gasheer-verwante faktore oor evolusionêre tyd gevorm is. / National Research Foundation (NRF)

2 pp. / This publication describes the common insects found in Arizona lettuce through the use of pictures. The insects include; lepidopterous larva, striped flea beetle, leafminer fly, leafminer mine, adult western flower thrips, winged adult aphid, false chinch bug, lygus bug, potato leafhopper, and threecornered alfalfa hopper.

Esta tese tem como objetivo principal a proposição de solução para o problema do encaixe de moldes em tecidos listrados da indústria do vestuário. Os moldes são peças com formato irregular que devem ser dispostos sobre a matéria-prima, neste caso o tecido, para a etapa posterior de corte. No problema específico do encaixe em tecidos listrados, o local em que os moldes são posicionados no tecido deve garantir que, após a confecção da peça, as listras apresentem continuidade. Assim, a fundamentação teórica do trabalho abrange temas relacionados à moda e ao design do vestuário, como os tipos e padronagens de tecidos listrados, e as possibilidades de rotação e colocação dos moldes sobre tecidos listrados. Na fundamentação teórica também são abordados temas da pesquisa em otimização combinatória como: características dos problemas bidimensionais de corte e encaixe e algoritmos utilizados por diversos autores para solucionar o problema. Ainda na parte final da fundamentação teórica são descritos o método Cadeia de Markov Monte Carlo e o algoritmo de Metropolis-Hastings. Com base na pesquisa bibliográfica, foram propostos dois algoritmos distintos para lidar com o problema de encaixe de moldes em tecidos listrados: algoritmo com pré-processamento e algoritmo de busca do melhor encaixe utilizando o algoritmo de Metropolis-Hastings. Ambos foram implementados no software Riscare Listrado, que é uma continuidade do software Riscare para tecidos lisos desenvolvido em Alves (2010). Para testar o desempenho dos dois algoritmos foram utilizados seis problemas benchmarks da literatura e proposto um novo problema denominado de camisa masculina. Os problemas benchmarks da literatura foram propostos para matéria-prima lisa e o problema camisa masculina especificamente para tecidos listrados. Entre os dois algoritmos desenvolvidos, o algoritmo de busca do melhor encaixe apresentou resultados com melhores eficiências de utilização do tecido para todos os problemas propostos. Quando comparado aos melhores resultados publicados na literatura para matéria-prima lisa, o algoritmo de busca do melhor encaixe apresentou encaixes com eficiências inferiores, porém com resultados superiores ao recomendado pela literatura específica da área de moda para tecidos estampados. / This thesis proposes the solution for the packing problem of patterns on striped fabric in clothing industry. The patterns are pieces with irregular form that should be placed on raw material which is, in this case, the fabric. This fabric is cut after packing. In the specific problem of packing on striped fabric, the position that patterns are put in the fabric should ensure that, after the clothing sewing, the stripes should present continuity. Thus, the theoretical foundation of this project includes subjects about fashion and clothing design, such as types and rapports of striped fabric, and the possibilities of rotation and the correct place to put the patterns on striped fabric. In the theoretical foundation, there are also subjects about research in combinatorial optimization as: characteristics about bi-dimensional packing and cutting problems and algorithms used for several authors to solve the problem. In addition, the Markov Chain Monte Carlo method and the Metropolis-Hastings algorithm are described at end of theoretical foundation. Based on the bibliographic research, two different algorithms for the packing problem with striped fabric are proposed: algorithm with pre-processing step and algorithm of searching the best packing using the Metropolis-Hastings algorithm. Both algorithms are implemented in the Striped Riscare software, which is a continuity of Riscare software for clear fabrics developed in the Masters degree of the author. Both algorithms performances are tested with six literature benchmark problems and a new problem called “male shirt” is proposed here. The benchmark problems of literature were iniatially proposed for clear raw material and the male shirt problem, specifically for striped fabrics. Between the two developed algorithms, the algorithm of searching the best packing has shown better results with better efficiencies of the fabric usage for all the problems tested. When compared to the best results published in the literature for clear raw material, the algorithm of searching the best packing has shown packings with lower efficiencies. However, it showed results higher than recommended for the specific literature of fashion design for patterned fabrics.