Michael Daugherty has created his niche in the music world by composing works inspired by icons of American popular culture. Red Cape Tango is the final movement of his Metropolis Symphony, a work inspired by the life and times of the comic book character Superman. This movement in particular deals with the death of the superhero through the use of musical elements, most notably the Latin Sequence of the Mass for the Dead, Dies irae. Daugherty's ingenuity in blending profoundly dark subjects with humor is particularly evident in this work. Death is personified as a temptress and lures Superman through the power of a seductive tango. This study concentrates on Daugherty's compositional style and its impact in musical circles. A transcription for wind orchestra was created by another composer/conductor precisely because of the need to bring such an important work to another medium, thus making it accessible to a wider audience. In addition, this study looks at the changes in instrumentation necessary to create a second, equally formidable version of the work.

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Informe de Seminario para optar al grado de Licenciado en Historia

It has been theorized that most proteins are under selection pressure to be soluble in crowded cellular spaces. To maintain solubility a proteins’ aggregation propensity should be inversely proportional to their maximum likely concentration. This theory was examined by comparing the proteome of the model organism S. cerevisiae, which has previously undergone a Whole Genome Duplication (WGD) event to the proteome of the closely related yeast K. waltii, which has not undergone WGD. This comparison revealed the following: 1) Predicted aggregation propensities are higher in S. cerevisiae than K. waltii. 2) Aggregation propensity does not predict which genes reverted to a single copy after WGD. 3) In genes which were retained as duplicates in S. cerevisiae after WGD, aggregation propensities rose from the inferred common ancestral protein. 4) Genes retained as duplicates showed less of an increase relative to their homologues in K. waltii than genes which were not retained as duplicates. 5) The relationship between the log predicted aggregation propensity and log mRNA expression level or log protein abundance was not linear as previously predicted. These results suggest that while there is broad selection pressure for reduced aggregation pressure for genes which have been duplicated, the precise relationship between aggregation propensity and gene expression is more complicated than previously predicted. These results also allow speculation that the whole genome duplication in S.cerevisiae may have been made possible by a general relaxation of aggregation-related selection pressure. / text

In quiescent yeast, the widespread reorganization of cytosolic proteins into punctate has been observed (Narayanaswamy et al. 2009). We seek to better understand and describe this reorganization, which we hypothesize to be a protein aggregation phenomenon. To test this hypothesis, we examined mutant proteins (Ade4p protein variants) in yeast with predicted non-native aggregation propensities and measured their punctate formation kinetics. Monitoring punctate formation kinetics involved the validation of an automated quantification technique using an Amnis ImageStream imaging flow cytometer. The automated punctate counts were strongly correlated with the manual punctate counts, with usual R² values of 0.99 or better, but evaluated 50-fold more cells per run. Fitness evaluations of the mutant yeast in the form of growth curves and batch competition experiments revealed the slowed growth of the Ade4-1286 strain and the functional inequality to the wild type strain of the Ade4-mtoin2034, Ade4-mtoin2105, and Ade4-2800 strains in competition experiments, especially when the mutants were forced to generate their own adenine. Subsequent structural analysis of the mutant proteins revealed destabilizing mutations for 4 of the 6 mutant proteins with 2 of the mutations classified as significantly destabilizing ([delta][delta]G >2 kcal/mol). We concluded that the reduction in protein fitness was likely due to the destabilizing effects of the mutations. Evaluation of the punctate formation kinetics revealed little difference between strains in the rate of punctate formation. Further examination revealed the wild type Ade4p and all of the mutants (with the exception of the Ade4-1286 mutant) were predicted to have similar aggregation propensities according to a secondary aggregation predicting algorithm (Zyggregator, Pawar et al. 2005). Additionally, solvent accessibility calculations estimate ~3-19% of the side chain surface area to be solvent accessible, which indicates proximity of mutations to the protein surface. However, mutating buried amino acids likely would have generated a greater disturbance (Matthews 1993, Tokuriki et al. 2007). We concluded that the mutations, although destabilizing, altered the aggregation propensity very little. Deletion of chaperone proteins (Hsp82p, Hsc82p, and Ssa1p) revealed no difference in the Ade4-GFP punctate formation kinetics, although a slight kinetic difference was detected in the chaperone (Hsp82p) knockout, Gln1-GFP strain and the wild type strain. While further workup is necessary in the chaperone knockout, Gln1-GFP work, the initial results are promising and suggest the involvement of protein folding machinery in punctate formation. / text

Buenos Aires. Metade do século dezenove. Uma política de fomento à imigração européia traz às terras portenhas mais de seis milhões de imigrantes. Ao contrário do que o governo argentino esperava, as que compunham esse cenário não eram pessoas requintadas, conhecedoras de arte e ciência, muito menos mão de obra qualificada. Os imigrantes de pouca instrução e de poucos meios que passaram a fazer parte da população residente nos arrabais portenhos, assolados pela saudade de suas terras, buscando no ambiente prostibulário por uma “natural” comunhão, dão origem ao tango nas últimas décadas do século dezenove. Por volta da mesma época, os irmãos Lumière produzem o invento que chamariam de cinematógrafo, palavra de raiz grega que significa “registro do movimento”. Portanto, percebo o tango e o cinema como expressões contemporâneas, no sentido de que ambas tem sua gênese ao final do mesmo século. Alguns/algumas historiadores/as apontam ainda que o cinema é um dos responsáveis pela valorização do tango e por seu reconhecimento mundial no decorrer do século passado. O que de certa forma sublinha a relação entre o cinema e a história. Sendo assim, esta dissertação de mestrado propõe analisar como os aspectos históricos do tango argentino são representados na película Tango de Carlos Saura. Usando o aporte teórico dos Estudos Culturais e entendendo as produções cinematográficas como artefatos pedagógicos, busco perceber quais são os sentidos produzidos sobre a história do tango argentino na narrativa construída por Saura. Inicio meu exercício analítico com um olhar atento sobre os elementos técnicos de cenário, iluminação e figurino empregados na construção da obra. Somado a isso, a linguagem cinematográfica utilizada por Saura, perceptível por meio dos planos, ângulos e movimentação de câmera, também são aspectos importantes que compreendem a análise. São quatro os momentos históricos do tango representados na película escolhidos para compor esse estudo. O período imigratório, quanto a sua relevância e envolvimento na gênese do tango, os duelos criollos e o tango dançado entre homens, o ambiente prostibulário e o tango entre mulheres e, por fim, o período da ditadura militar argentina em sua relação com o tango. Ao considerar esse último aspecto tento perceber ainda como outras películas abordam a memória do período, em especial quanto a temática do exílio. Por fim, proponho uma análise da dramaturgia da dança executada em cada uma das cenas a fim de perceber o que a linguagem coreográfica nos diz sobre o período que representa. / Buenos Aires. Half of the nineteenth century. A policy to promote european immigration to Buenos Aires brings more than six million immigrants. Contrary to the Argentine government hoped, composing this scenario were not intellectual people, knowledgeable of art and science, much less skilled labor. Immigrants with little education and few resources that have become part of the resident population in arrabais, covered by nostalgia for their land, in the brothel environment looking for a “natural” communion, give rise to tango in the last decades of the nineteenth century. Around the same time, the Lumière brothers produce the invention that would call cinematographer, Greek root word meaning "record of the movement." Therefore, realize the tango and cinema as contemporary expressions, in the sense that both have their genesis at the end of the same century. Some historians the still point that cinema is one responsible for the valuation of tango and its worldwide recognition during the last century. Which I think that somehow emphasizes the relationship between cinema and history. Thus, this dissertation aims to analyze how the historical aspects of Argentine tango are represented in Tango by Carlos Saura. Using the theoretical framework of Cultural Studies and understanding cinema as pedagogical artifacts, I seek to understand what are the meanings produced from the Argentine tango in the narrative constructed by Saura. First of all, my analytical exercise began with a look on technical elements of scenery, lighting and costumes. Added to this, the cinematographic language used by Saura, noticeable through the planes, angles and camera movements are also important aspects that comprise the analysis. There are four historical moments of the tango represented in saura’s movie, chosen to make this study. The period of immigration, as their relevance and involvement in the genesis of the tango, tango-dance executed by men, the brothel environment and tango between women and finally the period of Argentine military dictatorship in its relationship with the tango. In considering this latter aspect still try to understand how other films deal with the memory of the period, especially as the theme of exile. Finally, I propose an analysis of the dramaturgy of dance performed in each of the scenes in order to realize what a choreographic language tells us about the period it represents.

Este memorial crítico-reflexivo teve por objetivo refletir sobre os processos de elaboração do experimento artístico “Um tango em clave”, que foi desenvolvido a partir do encontro entre meu corpo e a clave (objeto de malabarismo), e apresentado em espaços de trânsito da cidade de Porto Alegre. Partindo da prática do tango, permeada pelo diálogo entre os pares, buscou-se uma relação na qual o objeto, a clave, torna-se materialidade pulsante que potencializa um diálogo a dois. Pretendia-se, assim, que a dança surgisse da relação entre esses dois corpos e não da manipulação de um corpo sobre o outro. Para tanto, o estudo encontra ressonância na possibilidade teórica de pensar em corpos não humanos como matérias vibrantes com potencial de provocar relações e movimentos. Dessa forma, esse encontro geraria a perda do eu, o qual seria construído através do movimento dançado. Ao longo dos ensaios e apresentações, realizaram-se registros escritos em caderno de notas, bem como registros fotográficas e videográficos. Eles tecem uma teia em conjunto com a escrita reflexiva e conceitual. Esse material possibilitou analisar a trajetória dessa proposta cênica. Essa pesquisa exploratória buscou contribuir para uma reflexão sobre as propostas de aprendizagem da dança, pois optou por um caminho de construção da relação dançada com o objeto, a partir das percepções cinestésicas do corpo em movimento. Tal procedimento sugere que outras metodologias de relação do corpo com o objeto possam ser desenvolvidas durante o processo de aprendizagem, e que elas podem ser tão eficientes quanto às práticas mais tradicionais de interação e manipulação do objeto. / This critical-reflexive memorial has the objective to reflect the process of elaboration of an artistic experiment “A tango in club” who was developed in the encounter of my body and the club (juggling key object) and has been showed in some places of transit in the city of Porto Alegre. The starting point has emerged from the experience of the artist in the practice of Tango from a dialogue bias between the peers, sought a relationship in which the object, becomes a pulsating materiality, a lively piece that will enhance a one-to-one conversation. It is thus intended that the dance results from the relationship between these two bodies and not from the manipulation of one body over another. Therefore, the study finds resonance on the theoretical possibility of thinking in non-human bodies as vibrant matters with capability to generate relationships and movements. In that way, this meeting would lead to a loss of the self, being constructed through the danced movement. Over the rehearsals and performances, there were written records in notebook, as well as photographic and videographic records. This information wove a web in conjunction with the reflective and conceptual writing. This exploratory research contribute to a reflection on the dance learning proposals because chose a path of building the relationship danced with the object, from the kinesthetic perception of the body in motion. This procedure suggests that other body regarding methodologies with the object can be developed during the learning process, and they can be as efficient as the more traditional practices of interaction and manipulation of the object.

abstract: Tres Tangos para Duo de Contrabajos (Three Tangos for Double Bass Duet) is a three-movement set written by Andrés Martín and commissioned by Darren Cueva specifically for this document and accompanying performance project. This piece blends tango with Western art music in a style often referred to as “nuevo tango” (new tango) which was popularized by Astor Piazzolla. This research paper will serve as a performance aid for those wishing to present tango idioms on the double bass in addition to a more detailed guide to performing Tres Tangos by Martín. To give context to performers, this survey begins with a brief history of the tango and the life and stylistic developments of Astor Piazzolla. Various music and dance styles that contributed to early tango include, milonga, habanera, and tango andalúz. The resulting tango was popularized as a music and dance style in the early twentieth century. Astor Piazzolla brought the tango to the concert hall after studying composition with acclaimed professor Nadia Boulanger. His new tango style merged traditional tango, classical composition, and jazz music, which he was exposed to after his family moved from Argentina to New York. Tres Tangos was modeled after the style of Piazzolla. Characteristic articulation and improvised techniques are a fundamental aspect of the tango sound; a successful performance will depend on the musician’s ability to create these sounds. A detailed description of the most common elements is provided as well as suggestions for creating them on the double bass. Finally, I have compiled a specific performance guide for Tres Tangos. This guide includes rhythmic, articulation, fingering, and notational considerations, to assist in the performance of this piece. / Dissertation/Thesis / Doctoral Dissertation Music 2018

Buenos Aires. Metade do século dezenove. Uma política de fomento à imigração européia traz às terras portenhas mais de seis milhões de imigrantes. Ao contrário do que o governo argentino esperava, as que compunham esse cenário não eram pessoas requintadas, conhecedoras de arte e ciência, muito menos mão de obra qualificada. Os imigrantes de pouca instrução e de poucos meios que passaram a fazer parte da população residente nos arrabais portenhos, assolados pela saudade de suas terras, buscando no ambiente prostibulário por uma “natural” comunhão, dão origem ao tango nas últimas décadas do século dezenove. Por volta da mesma época, os irmãos Lumière produzem o invento que chamariam de cinematógrafo, palavra de raiz grega que significa “registro do movimento”. Portanto, percebo o tango e o cinema como expressões contemporâneas, no sentido de que ambas tem sua gênese ao final do mesmo século. Alguns/algumas historiadores/as apontam ainda que o cinema é um dos responsáveis pela valorização do tango e por seu reconhecimento mundial no decorrer do século passado. O que de certa forma sublinha a relação entre o cinema e a história. Sendo assim, esta dissertação de mestrado propõe analisar como os aspectos históricos do tango argentino são representados na película Tango de Carlos Saura. Usando o aporte teórico dos Estudos Culturais e entendendo as produções cinematográficas como artefatos pedagógicos, busco perceber quais são os sentidos produzidos sobre a história do tango argentino na narrativa construída por Saura. Inicio meu exercício analítico com um olhar atento sobre os elementos técnicos de cenário, iluminação e figurino empregados na construção da obra. Somado a isso, a linguagem cinematográfica utilizada por Saura, perceptível por meio dos planos, ângulos e movimentação de câmera, também são aspectos importantes que compreendem a análise. São quatro os momentos históricos do tango representados na película escolhidos para compor esse estudo. O período imigratório, quanto a sua relevância e envolvimento na gênese do tango, os duelos criollos e o tango dançado entre homens, o ambiente prostibulário e o tango entre mulheres e, por fim, o período da ditadura militar argentina em sua relação com o tango. Ao considerar esse último aspecto tento perceber ainda como outras películas abordam a memória do período, em especial quanto a temática do exílio. Por fim, proponho uma análise da dramaturgia da dança executada em cada uma das cenas a fim de perceber o que a linguagem coreográfica nos diz sobre o período que representa. / Buenos Aires. Half of the nineteenth century. A policy to promote european immigration to Buenos Aires brings more than six million immigrants. Contrary to the Argentine government hoped, composing this scenario were not intellectual people, knowledgeable of art and science, much less skilled labor. Immigrants with little education and few resources that have become part of the resident population in arrabais, covered by nostalgia for their land, in the brothel environment looking for a “natural” communion, give rise to tango in the last decades of the nineteenth century. Around the same time, the Lumière brothers produce the invention that would call cinematographer, Greek root word meaning "record of the movement." Therefore, realize the tango and cinema as contemporary expressions, in the sense that both have their genesis at the end of the same century. Some historians the still point that cinema is one responsible for the valuation of tango and its worldwide recognition during the last century. Which I think that somehow emphasizes the relationship between cinema and history. Thus, this dissertation aims to analyze how the historical aspects of Argentine tango are represented in Tango by Carlos Saura. Using the theoretical framework of Cultural Studies and understanding cinema as pedagogical artifacts, I seek to understand what are the meanings produced from the Argentine tango in the narrative constructed by Saura. First of all, my analytical exercise began with a look on technical elements of scenery, lighting and costumes. Added to this, the cinematographic language used by Saura, noticeable through the planes, angles and camera movements are also important aspects that comprise the analysis. There are four historical moments of the tango represented in saura’s movie, chosen to make this study. The period of immigration, as their relevance and involvement in the genesis of the tango, tango-dance executed by men, the brothel environment and tango between women and finally the period of Argentine military dictatorship in its relationship with the tango. In considering this latter aspect still try to understand how other films deal with the memory of the period, especially as the theme of exile. Finally, I propose an analysis of the dramaturgy of dance performed in each of the scenes in order to realize what a choreographic language tells us about the period it represents.

Este memorial crítico-reflexivo teve por objetivo refletir sobre os processos de elaboração do experimento artístico “Um tango em clave”, que foi desenvolvido a partir do encontro entre meu corpo e a clave (objeto de malabarismo), e apresentado em espaços de trânsito da cidade de Porto Alegre. Partindo da prática do tango, permeada pelo diálogo entre os pares, buscou-se uma relação na qual o objeto, a clave, torna-se materialidade pulsante que potencializa um diálogo a dois. Pretendia-se, assim, que a dança surgisse da relação entre esses dois corpos e não da manipulação de um corpo sobre o outro. Para tanto, o estudo encontra ressonância na possibilidade teórica de pensar em corpos não humanos como matérias vibrantes com potencial de provocar relações e movimentos. Dessa forma, esse encontro geraria a perda do eu, o qual seria construído através do movimento dançado. Ao longo dos ensaios e apresentações, realizaram-se registros escritos em caderno de notas, bem como registros fotográficas e videográficos. Eles tecem uma teia em conjunto com a escrita reflexiva e conceitual. Esse material possibilitou analisar a trajetória dessa proposta cênica. Essa pesquisa exploratória buscou contribuir para uma reflexão sobre as propostas de aprendizagem da dança, pois optou por um caminho de construção da relação dançada com o objeto, a partir das percepções cinestésicas do corpo em movimento. Tal procedimento sugere que outras metodologias de relação do corpo com o objeto possam ser desenvolvidas durante o processo de aprendizagem, e que elas podem ser tão eficientes quanto às práticas mais tradicionais de interação e manipulação do objeto. / This critical-reflexive memorial has the objective to reflect the process of elaboration of an artistic experiment “A tango in club” who was developed in the encounter of my body and the club (juggling key object) and has been showed in some places of transit in the city of Porto Alegre. The starting point has emerged from the experience of the artist in the practice of Tango from a dialogue bias between the peers, sought a relationship in which the object, becomes a pulsating materiality, a lively piece that will enhance a one-to-one conversation. It is thus intended that the dance results from the relationship between these two bodies and not from the manipulation of one body over another. Therefore, the study finds resonance on the theoretical possibility of thinking in non-human bodies as vibrant matters with capability to generate relationships and movements. In that way, this meeting would lead to a loss of the self, being constructed through the danced movement. Over the rehearsals and performances, there were written records in notebook, as well as photographic and videographic records. This information wove a web in conjunction with the reflective and conceptual writing. This exploratory research contribute to a reflection on the dance learning proposals because chose a path of building the relationship danced with the object, from the kinesthetic perception of the body in motion. This procedure suggests that other body regarding methodologies with the object can be developed during the learning process, and they can be as efficient as the more traditional practices of interaction and manipulation of the object.