Le syndrome d’embolie de liquide amniotique (SELA) est une complication rare et souvent catastrophique de l’accouchement chez la femme caractérisée classiquement par une hypotension sévère, un arrêt cardiorespiratoire et une coagulation intra-vasculaire disséminée. Malheureusement, sa physiopathologie est encore mal connue. Le rôle des kinines n’a notamment pas été étudié. L’objectif de notre projet était de développer un modèle animal de SELA et d’étudier le rôle éventuel des kinines dans ce syndrome. Douze lapines en fin de gestation ont été incluses dans l’étude. Pour chacune d’entre-elles, le liquide amniotique était aspiré de chaque sac amniotique après une laparotomie. Six lapines recevaient un bolus de liquide amniotique injecté via la veine auriculaire alors que les six autres recevaient un bolus de saline. Parallèlement, les effets in vitro de liquide amniotique sur la coagulation étaient évalués par thrombelastographie (TEG) et comparés aux effets de la saline. L’injection de liquide amniotique n’a pas permis de reproduire les signes cliniques de SELA, n’a pas entrainé la génération de bradykinine, et n’a pas eu d’effet sur le temps de prothrombine, le temps de thromboplastine partielle activée, et l’activité du facteur VIII de. Une thrombocytopénie sévère et transitoire a cependant été notée 5 minutes après l’injection de liquide amniotique. De plus, en additionnant in vitro de liquide amniotique au sang on a observé un tracé de TEG hypercoagulable comparé à celui obtenu avec la saline. Le modèle n’ayant pas pu reproduire le SELA, le rôle des kinines dans ce syndrome reste à déterminer. / Amniotic fluid embolism (AFE) is a rare but catastrophic complication of parturition characterized by severe hypotension, cardiovascular collapse, and massive consumptive coagulopathy. Its pathophysiology remains obscure. In particular, the potential role of bradykinin in hypotension is unknown. The objective of this study was to develop a suitable animal model of AFE and to study the effects of amniotic fluid injection on bradykinin release in this model. Twelve rabbits in late gestation (25 days) were used. For each rabbit, amniotic fluid was collected from foetal amniotic sacs by laparotomy. For six rabbits, the amniotic fluid was then injected as a bolus via the left auricular vein, whereas the six other rabbits received saline (control group). In parallel, the in vitro effects of amniotic fluid on coagulation was assessed by thrombelastography (TEG) and compared to the effects of saline. Injection of amniotic fluid did not reproduce clinical signs of AFE, did not provoke bradykinin generation and had no effect on prothrombin time, the activated partial thromboplastin time, nor Factor VIII activity. However, a significant thrombocytopenia was observed five minutes after amniotic fluid administration. This thrombocytopenia resolved within 60 minutes. In vitro addition of amniotic fluid to blood resulted in accelerated clotting on TEG tracings as compared to the effect of saline. As we were not able to reproduce AFE with our model, the role of kinins in this syndrome remains to be determined.

INTRODUÇÃO: Dengue é uma doença viral prevalente e potencialmente fatal associada à alteração da permeabilidade capilar e coagulopatia. Entretanto, não há estudos concernentes aos achados tromboelastométricos nesta doença. Realizamos o presente estudo para analisar pacientes com dengue e plaquetopenia por meio de um exame rápido, efetivo e a beira leito comparando com os exames convencionais de coagulação. MÉTODOS: Trata-se de um estudo observacional e transversal conduzido entre os dias 6 de abril a 5 de maio de 2015, em São Paulo, Brasil, durante epidemia de dengue. Foi realizado tromboelastometria ROTEM® em 53 pacientes com dengue e trombocitopenia em associação com exames convencionais de coagulação: tempo de protrombina (TP), international normalized ratio (INR), tempo de tromboplastina parcial ativado (TTPa), tempo de trombina (TT), contagem de plaquetas, fibrinogênio e d-dímero. Um grupo controle de pacientes foi estabelecido para comparação do status tromboelastométrico. RESULTADOS: Um total de 38 pacientes de 53 (71,7%) apresentaram anormalidades no INTEM e 29/53 (57,4%) no EXTEM. Em contrapartida, alterações no FIBTEM foram encontradas apenas em 3/53 (5,7%). Houve significância estatística em pacientes correlacionando alterações tromboelastométricas no EXTEM e INTEM e contagem de plaquetas (p=0,052) e (p=0,005), respectivamente; assim como os valores de fibrinogênio (p=0,006) e (p=0,021), respectivamente. O grupo controle (GC) apresentou status tromboelastométrico normal em 10/10 (100%) na análise do INTEM, EXTEM, FIBTEM. Avaliação do EXTEM demonstrou significância estatística entre o GC e o grupo Dengue: CT (p=0,044); CFT (p<0,001); MCF (p < 0,001) e Alpha (p < 0,001). Foram observados níveis normais de fibrinogênio (mediana: 290) e altos níveis de d-dímero (mediana: 1330) com IQR (800-1840). Todos os pacientes (53/53) apresentavam trombocitopenia abaixo de 100 x 109/L (mediana 77 x 109/L) IQR (63-88). Exames convencionais de coagulação revelaram-se completamente normais: TP (mediana: 100%) IQR (90-100); INR (mediana: 1,0) IQR (1,0-1,1); TTPa (mediana: 28,9 segundos) IQR (26,0-32,5) e TT (mediana: 18,2 segundos) IQR (17,0-19,5). Apenas (7/49) 14,3% pacientes apresentaram sangramento e (3/52) 5,8% necessitou de hospitalização. Não houve associação entre alterações tromboelastométricas com sangramento ou hospitalização. CONCLUSÕES: Dengue representa um processo inflamatório intenso, mantendo níveis normais de fibrinogênio. Portanto, FIBTEM mantém-se normal promovendo boa formação do coágulo sem risco imediato de sangramento. Não houve correlação entre os achados tromboelastométricos com os exames convencionais de coagulação, sugerindo que testes viscoelásticos são exames mais sensíveis para análise de coagulopatia precoce nessa população / INTRODUCTION: Dengue is a prevalent and potentially fatal viral disease associated with plasma leakage and coagulopathy, though no information is available on thromboelastometric profile. We performed this study to analyze dengue fever patients with thrombocytopenia clot changes through point-ofcare thromboelastometry tests and standard coagulation tests. METHODS: This was an observational, transversal and cross sectional study conducted between April 6th and May 5th 2015 in São Paulo, Brazil, during a dengue outbreak. Thromboelastometry ROTEM® was performed in 53 patients with dengue and thrombocytopenia, in association with conventional coagulation tests: prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), thrombin time (TT); platelet count, fibrinogen level, and d-dimer. A control group of 10 patients was established to compare thromboelastometry profiles. RESULTS: A total of 38 patients in 53 (71,7%) had abnormalities in INTEM, 29 in 53 (57,4%) in EXTEM. Conversely, FIBTEM was abnormal in 3/53 (5,7%). Statistical analysis revealed significant relation in those patients with impairment EXTEM and INTEM with lowered platelet (p=0,052) and (p=0,005) respectively and lowered fibrinogen levels (p=0,006) and (p=0,021) respectively. Control group (CG) had normal status in 10/10 (100%) of INTEM, EXTEM, FIBTEM analysis. EXTEM analysis demonstrated statistical differences between CG and dengue group: CT (p=0,044); CFT (p < 0,001); MCF (p < 0,001) and Alpha (p < 0,001). Normal levels of fibrinogen (median: 290) and high levels of ddimer (median: 1330) IQR (800-1840) were found. All patients (53/53) had platelet under 100 x 109/L (median 77 x 109/L) IQR (63-88). Standard coagulation tests were completely normal: PT (median: 100%) IQR (90-100); INR (median: 1,0) IQR (1,0-1,1); aPTT (median: 28,9 seconds) IQR (26.0- 32,5) and TT (median: 18,2 seconds) IQR (17,0-19.5). Only (7/49) 14,3% patients had bleeding manifestations and (3/52) 5,8% needed hospitalization. There was no association between altered thromboelastometry with bleeding manifestations or hospitalization. CONCLUSIONS: Dengue represents an intense inflammatory process, maintaining normal levels of fibrinogen. FIBTEM remains normal providing good clot strength without immediate bleeding risk. There were no correlation between thromboelastometry findings and standard coagulation exams, suggesting that viscoelastic tests are more sensible method to analyze early coagulation impairments in this population

Introdução: Considerando que uma melhor vigilância do estado hemostático dos doentes antes, durante e após o ato cirúrgico pode ter impacto significativo na sua evolução, e sabendo que os testes clássicos da coagulação têm limitações para avaliar a hemóstase na globalidade, e presumindo-se que o ROTEM seja um teste que permite efetuar esta avaliação da coagulação, fizemos hipótese de que este método seria uma ferramenta que teria a habilidade de prever sangramento em cirurgia cardíaca valvar. Objetivos: 1) Verificar se o ROTEM (Tromboelastometria Rotacional) ao analisar o estado da coagulação sanguínea de pacientes submetidos à cirurgia cardíaca valvar tem a capacidade de prever maior risco de sangramento com suas consequentes complicações; 2) Correlacionar comorbidades e história clinica pré-existentes à cirurgia cardíaca valvar com o nível de sangramento apresentado. Métodos: Foram incluídos 100 pacientes consecutivos submetidos à cirurgia cardíaca valvar com circulação extracorpórea (CEC) nos seguintes procedimentos: cirurgia cardíaca valvar em uma ou mais valvas, incluindo reoperações e cirurgias combinadas, realizadas no Instituto do Coração (INCOR) do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo. Estudo prospectivo que buscou avaliar a eficácia do uso da tromboelastometria rotacional na previsão de sangramento em cirurgia cardíaca valvar. Após a indução anestésica foram coletados: Tromboelastometria, coagulograma, fibrinogênio, dímero D e contagem de plaquetas; com a finalidade de verificar potencial risco de sangramento neste paciente. Correspondente ao tempo - 0 (T0). Estes mesmos exames foram recoletados na admissão na Unidade de Terapia Intensiva Cirúrgica (UTIC), onde o paciente foi recebido após o procedimento cirúrgico cardíaco valvar. Correspondente ao tempo - 1 (T1). Optou-se pela coleta de T1 na UTI, pois a CEC já terá sido descontinuada e a heparina revertida com a administração da protamina. Resultados: Os pacientes fora subdivididos em quartis de acordo com o sangramento, configurando um total (n) de 100 pacientes com uma média de sangramento (débitos dos drenos) de 492,95 mL, apresentando um desvio padrão de 388,14 mL e 2260 mL. Não foi encontrada nenhuma variável estatisticamente significante entre os grupos, comparando-se exames laboratoriais pré-operatórios, tempo de CEC, tempo de pinçamento e uso de drogas vaso ativas. Porém, ocorreu diferença significativa (p = 0,015) nos níveis transfusionais de hemocomponentes entre os quartis. Ocorreu uma relação significante (p =0,014) entre o nível adequado de calcemia e tendência a menor sangramento nos grupos estudados. Os resultados do ROTEM - INTEM, ROTEM - EXTEM e ROTEM - FIBTEM não demonstraram diferença estatística significante entre os grupos estudados. Considerando-se os desfechos, baixo débito, choque cardiogênico, arritmia, AVC, insuficiência renal aguda, óbito e reoperação, apenas a reoperação apresentou resultados com diferença significante entre os grupos (p =0,024). Conclusões: 1- O ROTEM não demonstrou a capacidade de prever sangramento em cirurgia cardíaca valvar. 2 - Não houve correlação do sangramento apresentado com as comorbidades pré-existentes / INTRODUCTION: Considering that better monitoring of the haemostatic status of patients before, during and after the surgical procedure can have a significant impact on their evolution, and knowing that classical coagulation tests have limitations in assessing hemostasis overall, and assuming that ROTEM is a test that allows to perform this evaluation, we hypothesized that this method would be a tool that would have the ability to predict bleeding in valve heart surgery. OBJECTIVES: 1) To verify if the ROTEM (Rotational Thromboelastometry) when analyzing the blood coagulation status of patients submitted to valve heart surgery has the capacity to predict a greater risk of bleeding with its consequent complications; 2) To correlate pre-existing comorbidities and clinical history with valve heart surgery with the level of bleeding presented. METHODS: We included 100 consecutive patients submitted to cardiac valve surgery with cardiopulmonary bypass (CPB) in the following procedures: valvular heart surgery in one or more valves, including reoperations and combined surgeries performed at the Heart Institute of the University of São Paulo. It is a prospective study aimed at evaluating the efficacy of rotational thromboelastometry in the prediction of bleeding in valve heart surgery. After the anesthetic induction were collected: thromboelastometry, coagulogram, fibrinogen, D-dimer and platelet count, with the purpose of verifying potential risk of bleeding in this patient. These samples were defined as time - 0 (T0). These same exams were collected on admission to the Intensive Care Unit. These samples were defined as time - 1(T1 We chose to collect T1 in the ICU, because at this moment it is expected that the total reversal of anticoagulation has already occurred .. RESULTS: Patients were subdivided into quartiles according to bleeding, with a total of 100 patients with a mean bleed (drainage rates) of 492.95 mL. No statistically significant variables were found between the groups, comparing preoperative laboratory tests, CPB time, clamping time and use of vasoactive drugs. However, there was a significant difference (p = 0.015) in transfusion levels of blood components between the quartiles. There Abstract was a significant relationship (p = 0.014) between the adequate level of calcemia and tendency to less bleeding in the groups studied. The results of ROTEM - INTEM, ROTEM - EXTEM and ROTEM - FIBTEM did not show a statistically significant difference between the groups studied. Considering the outcomes, low rate, cardiogenic shock, arrhythmia, stroke, acute renal failure, death and reoperation, only reoperation presented results with significant difference between the groups (p = 0.024). CONCLUSIONS: 1 - ROTEM did not demonstrate the ability to predict bleeding in valvular heart surgery. 2 - There was no correlation of bleeding presented with pre-existing comorbidities

Le syndrome d’embolie de liquide amniotique (SELA) est une complication rare et souvent catastrophique de l’accouchement chez la femme caractérisée classiquement par une hypotension sévère, un arrêt cardiorespiratoire et une coagulation intra-vasculaire disséminée. Malheureusement, sa physiopathologie est encore mal connue. Le rôle des kinines n’a notamment pas été étudié. L’objectif de notre projet était de développer un modèle animal de SELA et d’étudier le rôle éventuel des kinines dans ce syndrome. Douze lapines en fin de gestation ont été incluses dans l’étude. Pour chacune d’entre-elles, le liquide amniotique était aspiré de chaque sac amniotique après une laparotomie. Six lapines recevaient un bolus de liquide amniotique injecté via la veine auriculaire alors que les six autres recevaient un bolus de saline. Parallèlement, les effets in vitro de liquide amniotique sur la coagulation étaient évalués par thrombelastographie (TEG) et comparés aux effets de la saline. L’injection de liquide amniotique n’a pas permis de reproduire les signes cliniques de SELA, n’a pas entrainé la génération de bradykinine, et n’a pas eu d’effet sur le temps de prothrombine, le temps de thromboplastine partielle activée, et l’activité du facteur VIII de. Une thrombocytopénie sévère et transitoire a cependant été notée 5 minutes après l’injection de liquide amniotique. De plus, en additionnant in vitro de liquide amniotique au sang on a observé un tracé de TEG hypercoagulable comparé à celui obtenu avec la saline. Le modèle n’ayant pas pu reproduire le SELA, le rôle des kinines dans ce syndrome reste à déterminer. / Amniotic fluid embolism (AFE) is a rare but catastrophic complication of parturition characterized by severe hypotension, cardiovascular collapse, and massive consumptive coagulopathy. Its pathophysiology remains obscure. In particular, the potential role of bradykinin in hypotension is unknown. The objective of this study was to develop a suitable animal model of AFE and to study the effects of amniotic fluid injection on bradykinin release in this model. Twelve rabbits in late gestation (25 days) were used. For each rabbit, amniotic fluid was collected from foetal amniotic sacs by laparotomy. For six rabbits, the amniotic fluid was then injected as a bolus via the left auricular vein, whereas the six other rabbits received saline (control group). In parallel, the in vitro effects of amniotic fluid on coagulation was assessed by thrombelastography (TEG) and compared to the effects of saline. Injection of amniotic fluid did not reproduce clinical signs of AFE, did not provoke bradykinin generation and had no effect on prothrombin time, the activated partial thromboplastin time, nor Factor VIII activity. However, a significant thrombocytopenia was observed five minutes after amniotic fluid administration. This thrombocytopenia resolved within 60 minutes. In vitro addition of amniotic fluid to blood resulted in accelerated clotting on TEG tracings as compared to the effect of saline. As we were not able to reproduce AFE with our model, the role of kinins in this syndrome remains to be determined.

INTRODUÇÃO: Dengue é uma doença viral prevalente e potencialmente fatal associada à alteração da permeabilidade capilar e coagulopatia. Entretanto, não há estudos concernentes aos achados tromboelastométricos nesta doença. Realizamos o presente estudo para analisar pacientes com dengue e plaquetopenia por meio de um exame rápido, efetivo e a beira leito comparando com os exames convencionais de coagulação. MÉTODOS: Trata-se de um estudo observacional e transversal conduzido entre os dias 6 de abril a 5 de maio de 2015, em São Paulo, Brasil, durante epidemia de dengue. Foi realizado tromboelastometria ROTEM® em 53 pacientes com dengue e trombocitopenia em associação com exames convencionais de coagulação: tempo de protrombina (TP), international normalized ratio (INR), tempo de tromboplastina parcial ativado (TTPa), tempo de trombina (TT), contagem de plaquetas, fibrinogênio e d-dímero. Um grupo controle de pacientes foi estabelecido para comparação do status tromboelastométrico. RESULTADOS: Um total de 38 pacientes de 53 (71,7%) apresentaram anormalidades no INTEM e 29/53 (57,4%) no EXTEM. Em contrapartida, alterações no FIBTEM foram encontradas apenas em 3/53 (5,7%). Houve significância estatística em pacientes correlacionando alterações tromboelastométricas no EXTEM e INTEM e contagem de plaquetas (p=0,052) e (p=0,005), respectivamente; assim como os valores de fibrinogênio (p=0,006) e (p=0,021), respectivamente. O grupo controle (GC) apresentou status tromboelastométrico normal em 10/10 (100%) na análise do INTEM, EXTEM, FIBTEM. Avaliação do EXTEM demonstrou significância estatística entre o GC e o grupo Dengue: CT (p=0,044); CFT (p<0,001); MCF (p < 0,001) e Alpha (p < 0,001). Foram observados níveis normais de fibrinogênio (mediana: 290) e altos níveis de d-dímero (mediana: 1330) com IQR (800-1840). Todos os pacientes (53/53) apresentavam trombocitopenia abaixo de 100 x 109/L (mediana 77 x 109/L) IQR (63-88). Exames convencionais de coagulação revelaram-se completamente normais: TP (mediana: 100%) IQR (90-100); INR (mediana: 1,0) IQR (1,0-1,1); TTPa (mediana: 28,9 segundos) IQR (26,0-32,5) e TT (mediana: 18,2 segundos) IQR (17,0-19,5). Apenas (7/49) 14,3% pacientes apresentaram sangramento e (3/52) 5,8% necessitou de hospitalização. Não houve associação entre alterações tromboelastométricas com sangramento ou hospitalização. CONCLUSÕES: Dengue representa um processo inflamatório intenso, mantendo níveis normais de fibrinogênio. Portanto, FIBTEM mantém-se normal promovendo boa formação do coágulo sem risco imediato de sangramento. Não houve correlação entre os achados tromboelastométricos com os exames convencionais de coagulação, sugerindo que testes viscoelásticos são exames mais sensíveis para análise de coagulopatia precoce nessa população / INTRODUCTION: Dengue is a prevalent and potentially fatal viral disease associated with plasma leakage and coagulopathy, though no information is available on thromboelastometric profile. We performed this study to analyze dengue fever patients with thrombocytopenia clot changes through point-ofcare thromboelastometry tests and standard coagulation tests. METHODS: This was an observational, transversal and cross sectional study conducted between April 6th and May 5th 2015 in São Paulo, Brazil, during a dengue outbreak. Thromboelastometry ROTEM® was performed in 53 patients with dengue and thrombocytopenia, in association with conventional coagulation tests: prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), thrombin time (TT); platelet count, fibrinogen level, and d-dimer. A control group of 10 patients was established to compare thromboelastometry profiles. RESULTS: A total of 38 patients in 53 (71,7%) had abnormalities in INTEM, 29 in 53 (57,4%) in EXTEM. Conversely, FIBTEM was abnormal in 3/53 (5,7%). Statistical analysis revealed significant relation in those patients with impairment EXTEM and INTEM with lowered platelet (p=0,052) and (p=0,005) respectively and lowered fibrinogen levels (p=0,006) and (p=0,021) respectively. Control group (CG) had normal status in 10/10 (100%) of INTEM, EXTEM, FIBTEM analysis. EXTEM analysis demonstrated statistical differences between CG and dengue group: CT (p=0,044); CFT (p < 0,001); MCF (p < 0,001) and Alpha (p < 0,001). Normal levels of fibrinogen (median: 290) and high levels of ddimer (median: 1330) IQR (800-1840) were found. All patients (53/53) had platelet under 100 x 109/L (median 77 x 109/L) IQR (63-88). Standard coagulation tests were completely normal: PT (median: 100%) IQR (90-100); INR (median: 1,0) IQR (1,0-1,1); aPTT (median: 28,9 seconds) IQR (26.0- 32,5) and TT (median: 18,2 seconds) IQR (17,0-19.5). Only (7/49) 14,3% patients had bleeding manifestations and (3/52) 5,8% needed hospitalization. There was no association between altered thromboelastometry with bleeding manifestations or hospitalization. CONCLUSIONS: Dengue represents an intense inflammatory process, maintaining normal levels of fibrinogen. FIBTEM remains normal providing good clot strength without immediate bleeding risk. There were no correlation between thromboelastometry findings and standard coagulation exams, suggesting that viscoelastic tests are more sensible method to analyze early coagulation impairments in this population

Introdução: Considerando que uma melhor vigilância do estado hemostático dos doentes antes, durante e após o ato cirúrgico pode ter impacto significativo na sua evolução, e sabendo que os testes clássicos da coagulação têm limitações para avaliar a hemóstase na globalidade, e presumindo-se que o ROTEM seja um teste que permite efetuar esta avaliação da coagulação, fizemos hipótese de que este método seria uma ferramenta que teria a habilidade de prever sangramento em cirurgia cardíaca valvar. Objetivos: 1) Verificar se o ROTEM (Tromboelastometria Rotacional) ao analisar o estado da coagulação sanguínea de pacientes submetidos à cirurgia cardíaca valvar tem a capacidade de prever maior risco de sangramento com suas consequentes complicações; 2) Correlacionar comorbidades e história clinica pré-existentes à cirurgia cardíaca valvar com o nível de sangramento apresentado. Métodos: Foram incluídos 100 pacientes consecutivos submetidos à cirurgia cardíaca valvar com circulação extracorpórea (CEC) nos seguintes procedimentos: cirurgia cardíaca valvar em uma ou mais valvas, incluindo reoperações e cirurgias combinadas, realizadas no Instituto do Coração (INCOR) do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo. Estudo prospectivo que buscou avaliar a eficácia do uso da tromboelastometria rotacional na previsão de sangramento em cirurgia cardíaca valvar. Após a indução anestésica foram coletados: Tromboelastometria, coagulograma, fibrinogênio, dímero D e contagem de plaquetas; com a finalidade de verificar potencial risco de sangramento neste paciente. Correspondente ao tempo - 0 (T0). Estes mesmos exames foram recoletados na admissão na Unidade de Terapia Intensiva Cirúrgica (UTIC), onde o paciente foi recebido após o procedimento cirúrgico cardíaco valvar. Correspondente ao tempo - 1 (T1). Optou-se pela coleta de T1 na UTI, pois a CEC já terá sido descontinuada e a heparina revertida com a administração da protamina. Resultados: Os pacientes fora subdivididos em quartis de acordo com o sangramento, configurando um total (n) de 100 pacientes com uma média de sangramento (débitos dos drenos) de 492,95 mL, apresentando um desvio padrão de 388,14 mL e 2260 mL. Não foi encontrada nenhuma variável estatisticamente significante entre os grupos, comparando-se exames laboratoriais pré-operatórios, tempo de CEC, tempo de pinçamento e uso de drogas vaso ativas. Porém, ocorreu diferença significativa (p = 0,015) nos níveis transfusionais de hemocomponentes entre os quartis. Ocorreu uma relação significante (p =0,014) entre o nível adequado de calcemia e tendência a menor sangramento nos grupos estudados. Os resultados do ROTEM - INTEM, ROTEM - EXTEM e ROTEM - FIBTEM não demonstraram diferença estatística significante entre os grupos estudados. Considerando-se os desfechos, baixo débito, choque cardiogênico, arritmia, AVC, insuficiência renal aguda, óbito e reoperação, apenas a reoperação apresentou resultados com diferença significante entre os grupos (p =0,024). Conclusões: 1- O ROTEM não demonstrou a capacidade de prever sangramento em cirurgia cardíaca valvar. 2 - Não houve correlação do sangramento apresentado com as comorbidades pré-existentes / INTRODUCTION: Considering that better monitoring of the haemostatic status of patients before, during and after the surgical procedure can have a significant impact on their evolution, and knowing that classical coagulation tests have limitations in assessing hemostasis overall, and assuming that ROTEM is a test that allows to perform this evaluation, we hypothesized that this method would be a tool that would have the ability to predict bleeding in valve heart surgery. OBJECTIVES: 1) To verify if the ROTEM (Rotational Thromboelastometry) when analyzing the blood coagulation status of patients submitted to valve heart surgery has the capacity to predict a greater risk of bleeding with its consequent complications; 2) To correlate pre-existing comorbidities and clinical history with valve heart surgery with the level of bleeding presented. METHODS: We included 100 consecutive patients submitted to cardiac valve surgery with cardiopulmonary bypass (CPB) in the following procedures: valvular heart surgery in one or more valves, including reoperations and combined surgeries performed at the Heart Institute of the University of São Paulo. It is a prospective study aimed at evaluating the efficacy of rotational thromboelastometry in the prediction of bleeding in valve heart surgery. After the anesthetic induction were collected: thromboelastometry, coagulogram, fibrinogen, D-dimer and platelet count, with the purpose of verifying potential risk of bleeding in this patient. These samples were defined as time - 0 (T0). These same exams were collected on admission to the Intensive Care Unit. These samples were defined as time - 1(T1 We chose to collect T1 in the ICU, because at this moment it is expected that the total reversal of anticoagulation has already occurred .. RESULTS: Patients were subdivided into quartiles according to bleeding, with a total of 100 patients with a mean bleed (drainage rates) of 492.95 mL. No statistically significant variables were found between the groups, comparing preoperative laboratory tests, CPB time, clamping time and use of vasoactive drugs. However, there was a significant difference (p = 0.015) in transfusion levels of blood components between the quartiles. There Abstract was a significant relationship (p = 0.014) between the adequate level of calcemia and tendency to less bleeding in the groups studied. The results of ROTEM - INTEM, ROTEM - EXTEM and ROTEM - FIBTEM did not show a statistically significant difference between the groups studied. Considering the outcomes, low rate, cardiogenic shock, arrhythmia, stroke, acute renal failure, death and reoperation, only reoperation presented results with significant difference between the groups (p = 0.024). CONCLUSIONS: 1 - ROTEM did not demonstrate the ability to predict bleeding in valvular heart surgery. 2 - There was no correlation of bleeding presented with pre-existing comorbidities