L’estradiol (E2) est une hormone femelle qui joue un rôle essentiel, à la fois dans la régulation et dans la détermination de certaines conditions physiologiques in vivo, telle que la différenciation et la prolifération cellulaire. Lorsque l’E2 est donné en supplément, par exemple dans le cas de thérapie hormonale, deux effets sont observés, un effet génomique et un effet non-génomique, de par son interaction avec les récepteurs à œstrogène du noyau ou de la membrane cellulaire, respectivement. L’effet non-génomique est plus difficile à étudier biologiquement parce que l’effet se produit sur une échelle de temps extrêmement courte et à cause de la nature hydrophobe de l’E2 qui réduit sa biodisponibilité et donc son accessibilité aux cellules cibles. C’est pourquoi il est nécessaire de développer des systèmes d’administration de l’E2 qui permettent de n’étudier que l’effet non-génomique de l’œstrogène. Une des stratégies employée consiste à greffer l’E2 à des macromolécules hydrophiles, comme de l’albumine de sérum bovin (BSA) ou des dendrimères de type poly(amido)amine, permettant de maintenir l’interaction de l’E2 avec les récepteurs d’œstrogène de la membrane cellulaire et d’éviter la pénétration de l’E2 dans le noyau des cellules. Toutefois, ces systèmes macromolécules-E2 sont critiquables car ils sont peu stables et l’E2 peut se retrouver sous forme libre, ce qui affecte sa localisation cellulaire. L’objectif de cette thèse est donc de développer de nouvelles plateformes fonctionnalisées avec de l’E2 en utilisant les approches de synthèses ascendantes et descendantes. Le but de ces plateformes est de permettre d’étudier le mécanisme de l’effet non-génomique de l’E2, ainsi que d’explorer des applications potentielles dans le domaine biomédical. L’approche ascendante est basée sur un ligand d’E2 activé, l’acide 17,α-éthinylestradiol-benzoïque, attaché de façon covalente à un polymère de chitosan avec des substitutions de phosphorylcholine (CH-PC-E2). L’estradiol est sous forme de pro-drogue attachée au polymère qui s’auto-assembler pour former un film. L’effet biologique de la composition chimique du film de chitosan-phosphorylcholine a été étudié sur des cellules endothéliales. Les films de compositions chimiques différentes ont préalablement été caractérisés de façon physicochimique. La topographie de la surface, la charge de surface, ainsi que la rhéologie des différents films contenant 15, 25, ou 40% molaires de phosphorylcholine, ont été étudiés par microscopie à force atomique (AFM), potentiel zêta, résonance plasmonique de surface et par microbalance à cristal de quartz avec dissipation (QCM-D). Les résultats de QCM-D ont montré que plus la part molaire en phosphorylcholine est grande moins il y a de fibrinogène qui s’adsorbe sur le film de CH-PC. Des cellules humaines de veine ombilicale (HUVECs) cultivées sur des films de CH-PC25 et de CH-PC40 forment des amas cellulaire appelés sphéroïdes au bout de 4 jours, alors que ce n’est pas le cas lorsque ces cellules sont cultivées sur des films de CH-PC15. L’attachement de l’estradiol au polymère a été caractérisé par plusieurs techniques, telles que la résonance magnétique nucléaire de proton (1H NMR), la spectroscopie infrarouge avec transformée de Fourier à réfraction totale atténuée (FTIR-ATR) et la spectroscopie UV-visible. La nature hydrogel des films (sa capacité à retenir l’eau) ainsi que l’interaction des films avec des récepteurs à E2, ont été étudiés par la QCM-D. Des études d’imagerie cellulaires utilisant du diacétate de diaminofluoresceine-FM ont révélé que les films hydrogels de CH-PC-E2 stimulent la production d’oxyde nitrique par les cellules endothéliales, qui joue un rôle protecteur pour le système cardiovasculaire. L’ensemble de ces études met en valeur les rôles différents et les applications potentielles qu’ont les films de type CH-PC-E2 et CH-PC dans le cadre de la médecine cardiovasculaire régénérative. L’approche descendante est basée sur l’attachement de façon covalente d’E2 sur des ilots d’or de 2 μm disposés en rangées et espacés par 12 μm sur un substrat en verre. Les ilots ont été préparés par photolithographie. La surface du verre a quant à elle été modifiée à l’aide d’un tripeptide cyclique, le cRGD, favorisant l’adhésion cellulaire. L’attachement d’E2 sur les surfaces d’or a été suivi et confirmé par les techniques de SPR et de QCM-D. Des études d’ELISA ont montré une augmentation significative du niveau de phosphorylation de la kinase ERK (marqueur important de l’effet non-génomique) après 1 heure d’exposition des cellules endothéliales aux motifs alternant l’E2 et le cRGD. Par contre lorsque des cellules cancéreuses sont déposées sur les surfaces présentant des motifs d’E2, ces cellules ne croissent pas, ce qui suggère que l’E2 n’exerce pas d’effet génomique. Les résultats de l’approche descendante montrent le potentiel des surfaces présentant des motifs d’E2 pour l’étude des effets non-génomiques de l’E2 dans un modèle in vitro. / Estradiol (E2) is an essential female hormone in the regulation and determination of various physiological conditions in vivo, such as cell proliferation and differentiation. When supplementing exogenous E2 as a clinical strategy for hormone therapy, it generates genomic and non-genomic effect simultaneously via binding to the estrogen receptors in the cell nucleus or membrane site. Compared to the genomic effect, it is quite difficult to monitor the E2-induced non-genomic biological behavior because this effect occurs in extremely transient time scale and the bioavailability and accessibility of E2 to target cells is very low due to the hydrophobic nature of E2. As a result, it is indispensable to develop E2 delivery systems to specifically understand estrogenic non-genomic nature. One of strategies is to graft E2 to the hydrophilic macromolecules, e.g. bovine serum albumin (BSA) or poly(amido)amine dendrimer, to maintain E2 interacting with membrane estrogen receptors instead of penetrating into the cell nucleus. However, the instability of those E2-macromolecules systems, either containing free E2 leaching or discrepancies of cellular localizations, led to controversies. Herein, the objective of present thesis is to develop novel E2-functionlized platforms by the principle of bottom-up and top-down approaches for understanding the mechanism of estrogenic non-genomic effect, and further, to explore their potential applications in the biomedicine. As a bottom-up approach, an activated E2 ligand, 17α-ethinylestradiol-benzoic acid was covalently conjugated onto a phosphorylcholine substituted chitosan polymer (CH-PC-E2) as a prodrug strategy for the fabrication of self-assembled films. Through a series of combined physicochemical and cellular investigations, the relationship between various chemical compositions of chitosan-phosphorylcholine (CH-PC) films and cellular responses was also evaluated. Based on atomic force microscopy (AFM) examination, zeta-potential measurements, surface plasmon resonance (SPR), and quartz crystal microbalance with dissipation (QCM-D) measurements, surface topography, charge, and rheology of CH-PC films with 15, 25, and 40 mol% PC contents were characterized. Moreover, QCM-D measurements indicated that the amount of fibrinogen adsorbed on CH-PC films decreased significantly with increasing PC content. Finally, it was also showed that human umbilical vein endothelial cells (HUVECs) form spheroids on CH-PC25 and CH-PC40 films, but not on CH-PC15 films cultured over 4 days. In addition, the CH-PC-E2 polymer conjugates were prepared and characterized by several techniques, such as 1H nuclear magnetic resonance (1H NMR), Fourier transformed infrared-attenuated total refraction (FTIR-ATR) and UV/Vis spectra measurements. The hydrogel nature of CH-PC-E2 film as well as its interactions to estrogen receptors was further extensively investigated by QCM-D study. In the cellular study, CH-PC-E2 hydrogel films can significantly stimulate the production of nitric oxide, a protective molecule in the cardiovascular system, in the endothelial cells by a diaminofluorescein-FM diacetate imaging study. The studies above demonstrated the different roles and potential applications of CH-PC-E2 and CH-PC surfaces in the cardiovascular regenerative medicine. As a top-down approach, micropatterned substrates were used for E2 functionalization, which were prepared by photolithography via aligning ~ 2 μm in diameter gold arrays onto a glass substrate. After that, a cell adhesive peptide, cyclic RGD was introduced to the glass surface in order to induce the attachment of cells. Meanwhile, estradiol was covalently immobilized on the gold surface and the process was monitored and validated by combining SPR and QCM-D studies. In the micropatterned substrate-coupled cell ELISA study, a phosphorylation level of extracellular signal-regulated kinase (ERK), which is an important non-genomic marker, was significantly elevated by this E2-functionalized micropatterned surface after 1 hour incubation. Furthermore, E2-functionalized micropatterned substrate didn’t proliferate cancer cells indicating the absence of genomic effect stimulation. Based on these results, our E2-functionalized micropatterned substrates can function as an in vitro model for the elucidation of estrogenic non-genomic behaviors.

This study sought to examine linguistic and discursive strategies used to construct messages reflective of the implementation of the HIV and AIDS policy for Zimbabwe of 1999 by government and private newspapers. Such analysis was perceived to be important since media content has a bearing on Zimbabweans‘ perception and attitudes regarding HIV and AIDS prevention, treatment and control. The study was aimed at comparing messages from newspapers with views by the people of Zimbabwe regarding the implementation of the policy. Findings reveal that empowerment programmes particularly those targeting women and children are lagging behind as Zimbabweans, literature and newspaper data sources testify. In addition, information sources concur that cultural (For example, stigmatisation, polygamy, religious practices, spouse inheritance) and structural (For example, patriarchy, masculinity, bureaucracy, politics) are stumbling blocks that negatively affect the implementation of the policy. Further, even though private and government newspapers do not fully agree on the portrayal of human agents, there is a general consensus between newspaper reports and Zimbabweans that people still face socio-economic and econo-political challenges that militate against the smooth implementation of the HIV and AIDS policy. Government newspapers tend to downplay aspects which reveal inadequacies of government activities. The study notes this as betrayal of use of ideological squares both by government and private newspapers whereby certain aspects regarding the implementation of the policy are either downplayed or highlighted to influence perception. The study reveals that newspaper reports used nominalisation, quantification, positive politeness, thematisation, rhematisation, intertextuality, euphemism, proverbs, idioms, action verbs, metaphors and citation of experts as linguistic and discursive strategies both for agenda setting and building purposes regarding the implementation of the HIV and AIDS policy. Other devices used particularly in the encoding of Operation Murambatsvina are, claptraps, deictic referencing, personal pronouns, adjectives and direct speech. The study attributes problems regarding the Zimbabwean HIV and AIDS intervention model to the top – down approach inherent in the policy. Hence, the call for an adoption of an unhu/hunhu/ubuntu inspired bottom – up HIV and AIDS intervention model in Zimbabwe. This would inculcate pro-family, pro-village, pro-nation/people and ―servant leadership‖ (Mangena and Chitando, 2011) values in the fight against the pandemic through the embracing of Indigenous Knowledge Systems (IKS). Unfortunately, such values largely continue to elude the radar of the current top – down HIV and AIDS intervention model cuurently in use in Zimbabwe. / African Languages / D. Litt et Phil. (African Languages)

Text in English / The study focuses on the interaction between two structures in development cooperation: the bottom-up approach of Informal Youth Groups (IYGs) and the topdown approach of International Non-Governmental Organisations (INGOs). These are considered in the theoretical context of the capability approach. In the empirical study, conducted in the cities of Amman and Zarqa in Jordan, the researcher interviewed eleven key members of IYGs to analyse their work mechanisms and criteria for co-operation. A Focus Group Discussion with members of INGOs about challenges and opportunities in co-operations with IYGs was conducted, to complement the literature review of INGO perspectives. The study provides insights into the work mechanism and co-operation criteria of IYGs. The interviewees mentioned trust between the parties and sharing vision and motivation as key requirements. Recommendations for INGOs in their work with local partners and practical steps for supporting IYGs are given. / Development Studies / M.A. (Development Studies)

<p>У докторату је предложен је нови модел за утврђивање деагрегационих<br />пропорција у top down методологији за хијерархијско прогнозирање.<br />Како би се утврдили показатељи рада новог приступа, извршена су<br />теоријска (симулациона студија) и емпиријска истраживања (студија<br />случаја) више ешалонског дистрибутивног ланца. Резултати показују да<br />нови приступ значајно превазилази стандардне моделе top down<br />методологије. Такође, у докторату је тестиран и утицај хијерархијских<br />прогноза на логистичке показатеље (просечне залихе и недостатак<br />залиха). Резултати показују да је нови модел остварио најмањи<br />недостатак залиха приликом примене у стратегијама управљања<br />залихама. Поред наведеног, у докторату је тестирано и комбиновање<br />различитих прогноза и истраживање утицаја особина временских серија<br />на прецизност прогнозирања модела за хијерархијско прогнозирање.</p> / <p>U doktoratu je predložen je novi model za utvrđivanje deagregacionih<br />proporcija u top down metodologiji za hijerarhijsko prognoziranje.<br />Kako bi se utvrdili pokazatelji rada novog pristupa, izvršena su<br />teorijska (simulaciona studija) i empirijska istraživanja (studija<br />slučaja) više ešalonskog distributivnog lanca. Rezultati pokazuju da<br />novi pristup značajno prevazilazi standardne modele top down<br />metodologije. Takođe, u doktoratu je testiran i uticaj hijerarhijskih<br />prognoza na logističke pokazatelje (prosečne zalihe i nedostatak<br />zaliha). Rezultati pokazuju da je novi model ostvario najmanji<br />nedostatak zaliha prilikom primene u strategijama upravljanja<br />zalihama. Pored navedenog, u doktoratu je testirano i kombinovanje<br />različitih prognoza i istraživanje uticaja osobina vremenskih serija<br />na preciznost prognoziranja modela za hijerarhijsko prognoziranje.</p> / <p>In this thesis, a new approach for determining disaggregating proportions in<br />the top down hierarchical forecasting methodology is proposed. In order to<br />estimate the accuracy of the proposed approach, the simulation and case<br />study are performed. Results demonstrate that the approach significantly<br />outperforms standard top down approaches. Also, in this reserach the impact<br />of hierarchical forecasts on logistics indicators (average stock and lack of<br />inventory) is researched. The results show that the new model achieved the<br />smallest lack of inventory in inventory management strategies. Likewise, in<br />this research, the ideas of combining the hierarchical forecasting models and<br />quantifying the influence of time series characteristics on the accuracy of<br />hierarchical forecasting models, are tested. The results are encouraging and<br />further researches are needed in order to reveal all possible benefits of<br />proposed ideas.</p>

本研究探討高齡者在視覺搜尋作業中，其由上而下（Top-down）與由下而上（Bottom-up）的注意力運作對於情緒刺激的反應，是否會因為注意力功能退化而與年輕成人的表現不同。過去研究指出，雖然高齡者注意力功能衰退，但是高齡者的由上而下注意力運作，仍可以幫助高齡者的表現。為了解由上而下注意力運作是否對於高齡者的反應有所幫助，本研究以兩個實驗分別探討內因與外因線索以及操弄訊息量所造成的影響。 實驗一包括80％有效的內因線索與25％有效的外因線索兩階段，外因線索階段為由下而上注意力運作，內因線索階段則為由上而下注意力運作，獨變項為年齡（高齡者、年輕成人）、情緒（生氣、快樂）以及線索有效情況（有效線索、中性線索、無效線索），記錄反應時間與正確率。實驗二的兩階段使用不同訊息量的外因線索（80％有效比例為高訊息量、25％有效比例為低訊息量），其餘操弄都與實驗一相同，低訊息量為單純的由下而上注意力運作，而高訊息量兼具由下而上與由上而下注意力運作。 研究結果發現，兩實驗高齡者與年輕成人都對於生氣情緒反應較快，符合威脅優勢。高齡者較年輕人更需要借助由上而下的注意力運作幫助反應，高訊息量的線索對於高齡者幫助也較年輕成人大。另外，實驗一增益效果的結果顯示，有效線索在高齡者搜尋生氣臉孔幫助較大，在年輕成人則搜尋快樂臉孔幫助較大，但是實驗二結果則顯示，有效線索在高齡者與年輕成人搜尋快樂臉孔的幫助都較生氣臉孔大，兩實驗的年齡與情緒的交互作用結果不同可能與高齡者異質性有關。由研究結果可知，由上而下注意力運作對於高齡者在情緒臉孔反應有幫助，在未來實務上，可以多運用由上而下注意力運作的線索幫助高齡者反應。 / This research is aim to understand attention in visual search of emotional faces in aging. Two types of attention process, that is top-down and bottom-up attention, were concerned in this study. In past researches, there are empirical evidences showing age-related declination in attention function, especially in bottom-up attention. But top-down attention is still playing an important role in doing task for the older adults. In order to understand the effect of top-down attention in aging, two experiments using endogenous or exogenous spatial cues were designed. Participants have to respond whether there is a different emotional face or not in a visual search task. The independent variables are age（aging and young）, emotional face（happy and angry）, and cue（valid, neutral, and invalid）. Dependent variables are reaction times and accuracy. In Experiment 1, there are two stages: 80％ valid endogenous cues and 25％ valid exogenous cues. The endogenous cues stage and exogenous cues stage were designed to probe top-down attention and bottom-up attention, respectively. In Experiment 2, there are two stages: 80％ valid exogenous cues（highly informative cues）and 25％ valid exogenous cues（non-informative cues）. Using highly informative exogenous cues involve both top-down and bottom-up attention. In contrast, non-informative exogenous cues only trigger bottom-up attention. The major results of this study support threat advantage hypothesis that both older and young adults respond to angry face target quicker than happy face. Benefit from top-down attention was larger in older adults than young adults. Highly informative cues help older adults more in searching emotional faces than young adults. The cuing effects in two emotional face conditions were different between Experiment 1 and 2. This inconsistent result maybe relates to aging heterogeneity. In conclusion, top-down attention can help older adults search for emotional faces.

El objetivo de esta tesis es estudiar el efecto del enriquecimiento por materia orgánica producida por el cultivo de peces en el mar y por los vertidos de petróleo en el medio marino. Este trabajo intenta explicar las consecuencias ecológicas de los impactos generados por este tipo de contaminación, y en diseñar de test de toxicidad para evaluar la contaminación en el sur de las costas europeas. Específicamente, en relación con el impacto de la acuicultura, los estudios de recuperación durante la Apertura de peces bentónicos de reducción de piscicultura marina, el papel de la depredación en el sistema bentónico dispersión de los residuos y los vínculos horizontales y verticales con un impacto bentónico. / The aim of this thesis is to study the effect of organic matter enrichment produced by marinefinfish farming and oil spills on the marine environment. This work is focused on elucidating theecological consequences of the impact generated by this type of pollution, and on designingtoxictity tests to evaluate pollution for southern European coasts. Specifically, related with aquaculture impact, it studies benthic recovery during open sea fish farming abatement, the role of predation in the benthic system and links horizontal and vertical waste dispersion with benthic impact.

No description available.

In this thesis I assessed the causes of long-term changes in two large, commercially important decapod crustacean populations, American lobster (Homarus americanus) and snow crab (Chionoecetes opilio), in the northwest (NW) Atlantic Ocean. By combining available time-series data, including commercial landings, research surveys, and local ecological knowledge (LEK), I explored the causes of an observed ecosystem shift in the NW Atlantic (~1950–2009) which entailed a region-wide decline of groundfish and an increase in benthic invertebrates, including these decapods. Three hypotheses were examined to explain the increase in decapod abundance: (1) the predation hypothesis, whereby a decrease in predatory groundfish led to an increase in their decapod prey (top-down effects); (2) the climate hypothesis, whereby changes in temperature or other climatic variables helped to increase decapod numbers (bottom-up effects); and (3) the anthropogenic hypothesis, whereby changes in fishing pressure drove decapod population dynamics. I explored these hypotheses separately for lobster and snow crab, which may experience different ecological and commercial pressures. First, I investigated the interactions between predatory groundfish and lobster in the inshore region of southwest Nova Scotia. Long-term fisheries-independent abundance indices for lobsters and their predators are available for Gulf of Maine (GOM) waters in the USA, but not in Canada. To address research gaps I designed and executed a survey to collect the LEK of lobster fishermen fishing in the Canadian GOM. Forty-two fishermen were interviewed. Corresponding survey results from the USA were compared to the LEK results. Both sources provided evidence for a top-down effect (predation release), contributing to observed increases in GOM lobster abundance and landings. Second, I explored relationships between lobster abundance and landings in the NW Atlantic as they may relate to temporal changes in predators, temperature, climate (North Atlantic Oscillation Index, NAOI), and fishing. Available landings data and fisheries-independent abundance estimates were collated to investigate trends in lobster abundance and catch. Links between lobster, groundfish, temperature and climate indices were explored using mixed effects models. Results offered partial support for the predation hypothesis, namely in the waters off Newfoundland, Nova Scotia, and southern New England as well as broad support for a climate effect on early life stages. This effect appeared related to a region-wide climate signal, the NAOI, but was independent of changes in water temperature. Fishing effort appeared to be following lobster abundance, rather than regulating abundance in a consistent way. Third, variation in snow crab abundance was examined through meta-analysis of time-series data of cod and crab abundance and temperature. Temperature had opposing effects on the two species: snow crab abundance was negatively correlated with temperature whereas cod and temperature were positively related. Controlling for the effect of temperature, the analysis revealed significant negative interactions between snow crab and cod abundance, with cod leading snow crab up to a five-year lag. Results indicate that snow crab is largely influenced by temperature during early post-settlement years and becomes increasingly regulated by top-down mechanisms as they approach fishery recruitment. Overall, I conclude that both climate and predation can act as population controls on large decapod populations, but these variables affect decapods at different life stages.

過去的研究指出，具有威脅訊息的情緒臉孔會比正向情緒臉孔快速地吸引個體的注意力。視覺搜尋研究發現情緒臉孔的突顯程度會引發由下而上的注意力攫取，而由下而上的注意力攫取是否能夠被由上而下的因素所凌駕，一直是一個很重要的論爭，因此，本研究同時呈現兩種情緒臉孔，並以文字線索引發由上而下注意力選擇，以探討由上而下的因素是否能夠凌駕情緒臉孔之注意力攫取，並比較不同情緒臉孔攫取注意力的強度。實驗一探討文字線索是否能夠幫助注意力搜尋目標情緒臉孔。實驗二使用一致效果當作注意力攫取指標，探討當臉孔情緒與作業相關時，由上而下因素是否能夠凌駕干擾情緒臉孔為生氣臉孔或快樂臉孔之注意力攫取，以及比較生氣與快樂臉孔攫取注意力的強度。實驗三延續實驗二的研究，將臉孔情緒改為與作業無關，比較生氣臉孔與快樂臉孔攫取注意力的強度。實驗四與實驗二實驗程序相同，但增加倒立中性臉孔與生氣臉孔或快樂臉孔同時呈現情況，如此在以倒立中性臉孔為目標或干擾臉孔情況，可以比較生氣臉孔與快樂臉孔之由上而下因素注意力選擇與由下而上注意力攫取強度。本研究結果在大部分情況下並未獲得一致效果，顯示由上而下之注意力選擇會影響由下而上之情緒臉孔攫取注意力，使得干擾臉孔未能吸引注意力於其上。然而由下而上注意力攫取仍影響搜尋情緒臉孔的反應時間，結果顯示生氣臉孔與快樂臉孔競爭下，生氣臉孔的突顯程度大於快樂臉孔，說明生氣臉孔較快樂臉孔吸引注意力。 / Previous studies using visual search task showed that the saliency of the emotional faces was sufficient to induce the bottom-up attentional capture. It is a critical debate that whether top-down factors can override bottom-up attentional capture. To address this issue, we presented two emotional faces at the same display while using word cues to induce top-down attentional selection. Experiment 1 investigated the effect of word cues on the target face searching. Using congruency effect as an index of attentional capture, Experiment 2 investigated whether top-down factors could override attentional capture of the emotional distractors when emotion is task-relevant and the degree of attentional capture of angry faces was compared with that of happy faces. In Experiment 3, face emotion was changed to be task-irrelevant rather than task-relevant, and the degree of attentional capture of angry and happy faces was also contrasted. In Experiment 4, the invert neutral face was included additionally as another target or distractor. We can use the invert neutral face as the common basis to dissociate the components of top-down attentional selection and bottom-up attentional capture, and contrast the attention effects between angry and happy faces in these two aspects independently. The results showed that congruency effect could not be found in most conditions. This implies that top-down attentional selection can influence the bottom-up attentional capture of emotional faces. But reaction time for searching target was still found to be influenced by bottom-up process. When an angry face competes with a happy face, the saliency of the angry face is higher than the happy face.

Cette thèse s’intéresse à la relation complexe entre processus ascendants (bottom-up) et processus descendants (top-down) dans la compréhension de l’oral L2 ; c’est-à-dire entre l’utilisation du signal sonore et de l’input linguistique d’une part, et l’intégration de connaissances diverses (linguistiques, discursives, pragmatiques, générales) d’autre part. Malgré de nombreux travaux en psycholinguistique, en acquisition des langues étrangères (L2) et en didactique (par exemple, Cutler & Clifton, 1999 ; Field, 2008; Rost, 2002 ; Brown, 1990), notre connaissance des processus cognitifs complexes de l’écoute ainsi que l’effet de certains facteurs sur la compréhension de l’oral restent à approfondir. Une appréciation plus fine de ces processus est indispensable aux questions relatives à l’enseignement et à l’apprentissage de la compréhension de l’oral en L2.Partant du principe que l’écoute a la même architecture cognitive en L1 et L2, nous commençons par un résumé – et une synthèse – des modèles psycholinguistiques récents de la compréhension de l’oral en L1. Nous examinons également les principaux facteurs rendant la compréhension difficile. Notre résumé des études en L2 consacrées au rôle de l'information descendante et au comportement stratégique des auditeurs L2 souligne la contribution importante de la métacognition. Nous tenterons donc ici de clarifier un certain flou terminologique qui caractérise ces travaux, et nous proposerons un modèle qui fait part de la métacognition dans la compréhension unidirectionnelle de l’oral en L2. Nous présentons ensuite les résultats d'une étude que nous avons menée pour analyser la contribution exacte de certains facteurs à l'écoute en L2.Les participants à cette étude sont des enseignants (n=23) et surtout des apprenants (n=226) français et tunisiens de l’anglais. En s’appuyant sur des méthodes multiples, à la fois quantitatives (différent tests et questionnaires) et qualitatives (questionnaires, tâches de réflexion à haute voix - Ericsson & Simon, 1993 - et de dévoilement graduel - gating, Grosjean, 1980), nous nous interrogeons: 1) sur les facteurs perçus par les apprenants et les enseignants comme étant déterminants dans la compréhension de l’anglais oral ; 2) sur la contribution relative de la compétence linguistique en L2, la discrimination auditive, la reconnaissance lexicale, et les compétences en méta-compréhension à une compréhension auditive réussie; 3) sur les problèmes que rencontrent nos auditeurs L2 lors de l’écoute, et sur les comportements stratégiques adoptés pour y faire face. Nous regardons plus particulièrement (pour ces différents paramètres) les différents niveaux de compétence en compréhension des sujets (avec une analyse poussée des auditeurs compétents et des auditeurs moins-compétents), ainsi qu’à de possibles influences de deux langues maternelles distinctes (français vs arabe tunisien). Nos analyses montrent : 1) ce que les apprenants et les enseignant perçoivent comme facteurs influant la compréhension de l’oral diffère relativement de ce qui la rend réellement problématique; 2) que la reconnaissance des mots et la connaissance lexicale contribuent significativement à la variation dans la compréhension, avec la reconnaissance des mots étant le prédicteur le plus fort ; 3) que les problèmes rencontrés en temps réel sont principalement de bas-niveau (segmentation de la parole), et que si les stratégies généralement contribuent à la compréhension, elles ne sont pas discriminatoires. Ce qui distingue donc l’auditeur expert du novice est son traitement formel (plus efficace et automatique) et non pas stratégique de l’information orale. Ces résultats sont discutés en rapport avec notre cadre théorique et selon une perspective pédagogique. / This thesis focuses on the complex relationship between bottom-up and top-down processes in L2 speech comprehension; i.e. between the use of the signal and the linguistic input on one hand, and the integration of various types of knowledge (linguistic, discourse, pragmatic, general) on the other hand. Despite a large body of research on the cognitive processes underlying listening in psycholinguistics, foreign language (L2) acquisition and teaching (e.g., Cutler & Clifton, 1999; Field, 2008a; Rost, 2002; Brown, 1990), there are still gaps in our understanding of these processes and the impact certain factors have on listening comprehension. Assuming that L1 and L2 listening follow the same cognitive architecture, we first review recent psycholinguistic models of L1 listening. We also examine the main factors constraining L2 listening comprehension. As our summary of the few SLA studies that have investigated the role of bottom-up information and the strategic behavior of L2 listeners points to the important contribution of metacognition, we clarify the terminological fuzziness characterizing this concept, and propose a model of metacognition in real-world unidirectional L2 listening. We then present the results of a study that we conducted to investigate the exact contribution of these different factors to L2 listening. The participants in this study were EFL French and Tunisian teachers (n=23) and learners (n=226). Using mixed quantitative (different tests and questionnaires) and qualitative (protocol analysis and gating experiments - Ericsson & Simon, 1993; Grosjean, 1980) methods, our aim was to investigate: 1) the factors perceived by learners and teachers as influencing L2 listening; 2) the relative contribution of linguistic knowledge, auditory discrimination, spoken word recognition (SWR), and meta-comprehension knowledge to successful L2 listening; 3) on-line listening problems and strategy use. For all of these parameters, we looked more closely at different levels of listening proficiency (various analyses of the performance of skilled and unskilled L2 listeners), as well as the possible influence of the two L1s (French and Tunisian Arabic) involved in the study.Our analyses show that: 1) there is a general discrepancy between what is perceived as making L2 listening difficult and what really renders it problematic; 2) SWR and vocabulary knowledge contribute significantly to the variance in L2 listening, with SWR being a stronger predictor; 3) listening problems encountered on-line are mainly lower-level (segmentation) and, although strategies contribute to speech comprehension, they are not discriminatory. What characterizes a proficient L2 listener seems to be accurate formal processing, not strategic processing of oral input. The findings are discussed from a theoretical and pedagogical perspective. Keywords: listening comprehension, French and Tunisian learners of L2 English, bottom-up and top-down processes, formal processing, integration and situation model, attentional resources, gating, protocol analysis, comparative analysis