Flavonoids are of great interest for their antioxidant and health-promoting activities. Ascorbate (vitamin C) has antioxidant activities but also sometimes displays pro-oxidant activities <i>in vitro</i> and reportedly <i>in vivo</i>. This research investigated to what extent flavonoids moderate oxidative stress from vitamin C <i>in vivo</i>.<p>Dietary experiments were conducted in two phases using adult male Wistar rats. First, all animals were maintained for two weeks on a control flavonoid-free diet with the dietary requirement (27 IU) of vitamin E/kg diet. In the subsequent four weeks, the animals were treated in four groups (8 rats/group), being fed the following diets: flavonoid-free control (C), ascorbate-supplemented (7.55 mmol/kg diet) (A), flavonoid-supplemented (2.67 mmol/kg diet) (F) and flavonoids (2.67 mmol/kg diet) plus ascorbate (7.55 mmol/kg diet)-supplemented (T). Measurements were done on in vivo biomarkers of oxidative stress, tissue antioxidants and on tissue in vitro susceptibility to oxidative stress.<p>In the combined feeding of ascorbate plus flavonoids, endogenous thiobarbituric acid reactive substances (TBARS) increased in liver by 114%. No effects of dietary ascorbate or flavonoids were seen on endogenous TBARS in brain or heart, or on plasma thiols or erythrocyte fragility.<p><i>In vitro</i>, the susceptibility to TBARS formation of liver homogenate (incubated for 60 min at 37ºC in air) showed a significant 60% increase in ascorbate-fed animals compared to control, but no increase in animals fed ascorbate plus flavonoids, suggesting that the additional feeding of flavonoids helped to prevent the increase produced by ascorbate-feeding. Incubation of liver mitochondria with 300 µM ascorbate in vitro produced a large (2-7 fold) increase in TBARS, but there was no difference among mitochondria from the different feeding groups.<p>The ability of flavonoid-feeding in protecting against oxidative stress from ascorbate in vivo could not be demonstrated in this study, even showing pro-oxidant effects of flavonoids in combination with ascorbate in liver. However, in vitro tests in liver suggest a protective effect of flavonoid-feeding against susceptibility to oxidative stress from ascorbate. Further investigations are needed in order to resolve the differences observed in vitro and in vivo and to determine the endogenous effects of specific flavonoids under ascorbate-induced oxidative stress.

Flavonoids are of great interest for their antioxidant and health-promoting activities. Ascorbate (vitamin C) has antioxidant activities but also sometimes displays pro-oxidant activities <i>in vitro</i> and reportedly <i>in vivo</i>. This research investigated to what extent flavonoids moderate oxidative stress from vitamin C <i>in vivo</i>.<p>Dietary experiments were conducted in two phases using adult male Wistar rats. First, all animals were maintained for two weeks on a control flavonoid-free diet with the dietary requirement (27 IU) of vitamin E/kg diet. In the subsequent four weeks, the animals were treated in four groups (8 rats/group), being fed the following diets: flavonoid-free control (C), ascorbate-supplemented (7.55 mmol/kg diet) (A), flavonoid-supplemented (2.67 mmol/kg diet) (F) and flavonoids (2.67 mmol/kg diet) plus ascorbate (7.55 mmol/kg diet)-supplemented (T). Measurements were done on in vivo biomarkers of oxidative stress, tissue antioxidants and on tissue in vitro susceptibility to oxidative stress.<p>In the combined feeding of ascorbate plus flavonoids, endogenous thiobarbituric acid reactive substances (TBARS) increased in liver by 114%. No effects of dietary ascorbate or flavonoids were seen on endogenous TBARS in brain or heart, or on plasma thiols or erythrocyte fragility.<p><i>In vitro</i>, the susceptibility to TBARS formation of liver homogenate (incubated for 60 min at 37ºC in air) showed a significant 60% increase in ascorbate-fed animals compared to control, but no increase in animals fed ascorbate plus flavonoids, suggesting that the additional feeding of flavonoids helped to prevent the increase produced by ascorbate-feeding. Incubation of liver mitochondria with 300 µM ascorbate in vitro produced a large (2-7 fold) increase in TBARS, but there was no difference among mitochondria from the different feeding groups.<p>The ability of flavonoid-feeding in protecting against oxidative stress from ascorbate in vivo could not be demonstrated in this study, even showing pro-oxidant effects of flavonoids in combination with ascorbate in liver. However, in vitro tests in liver suggest a protective effect of flavonoid-feeding against susceptibility to oxidative stress from ascorbate. Further investigations are needed in order to resolve the differences observed in vitro and in vivo and to determine the endogenous effects of specific flavonoids under ascorbate-induced oxidative stress.

In order to develop an environmentally friendly seaweed extract treatment which will benefit both pre- and post-harvest qualities of vegetables, the effects of pre-harvest application of the brown algae Ascophyllum nodosum extracts on the nutritional quality and post-harvest storability of spinach (Spinacia oleracea L.) was investigated. Plants treated with A. nodosum extracts accumulated higher concentrations of iron, potassium, total soluble protein, and total phenolics as compared to untreated controls. 1H NMR and LC-MS analysis revealed a roughly 50% enhanced accumulation of the 9 flavonoids identified, which is partially confirmed by the elevated chalcone isomerase activity. A. nodosum extract treatment caused an increase in transcription of the genes related to plant growth, osmolyte accumulation, and antioxidative activities. Post-harvest analysis revealed that A. nodosum extract treatment caused an enhanced storability of spinach leaves in terms of visual quality, weight loss, and senescence. Lipid peroxidation and ascorbate content were correlated with visual quality during storage. Animal experiments using the Caenorhabditis elegans nematode model revealed that spinach extracts prolonged the life span of C. elegans, and A. nodosum extract-enhanced polyphenols exerted improved beneficial effects in C. elegans against oxidative and heat stresses. Taken together, the results suggest that A. nodosum extracts enhance both pre- and post-harvest quality of spinach through stimulation of flavonoid pathways, thus leading to accumulation of flavonoids and promotion of anti-radical capacity in spinach leaves, which may protect the plant tissue against reactive oxygen species and subsequent decay. Furthermore, the increased flavonoid content in spinach exerted beneficial effects in C. elegans against oxidative and heat stresses via different mechanisms.

This thesis deals with determination of content of vitamin C in various types of cornelian cherry (Cornus mas L.). Some basic information about Cornus mas L. and its various types are described in the literary part of this thesis. The summary of important components present at fruit and some possibilities of processing and utilization are described here too. Various methods of determination of vitamin C are presented at the end of the literary part. The main result of the experimental part of this thesis is the comparison of content of vitamin C in various types of cornelian cherry (Cornus mas L.). Reversed Phase-High Performance Liquid Chromatography was used as the main method. HPLC method of determination of vitamin C in various types of Cornus mas L. was completed with the orientation titration. Then total antioxidant capacity of fruit was evaluated by using of reaction of extracts of fruit with stable radical DPPH. The biggest amount of vitamin C was determined in the Fruchtal variety. The biggest total antioxidant capacity was determined in the Vydubecký variety.

ABREU, Cyntia Rafaelle Amaral de. Qualidade e atividade antioxidante total de pedúnculos de clones comerciais de cajueiro anão precoce. 2007. 111 f. : Dissertação (mestrado) - Universidade Federal do Ceará, Centro de Ciências Agrárias, Departamento de Tecnologia de Alimentos, Fortaleza-CE, 2007 / Submitted by Nádja Goes (nmoraissoares@gmail.com) on 2016-06-01T13:09:44Z No. of bitstreams: 1 2007_dis_craabreu.pdf: 1760977 bytes, checksum: 9089d14f4e32ad467697dbb14559502f (MD5) / Approved for entry into archive by Nádja Goes (nmoraissoares@gmail.com) on 2016-06-01T13:09:56Z (GMT) No. of bitstreams: 1 2007_dis_craabreu.pdf: 1760977 bytes, checksum: 9089d14f4e32ad467697dbb14559502f (MD5) / Made available in DSpace on 2016-06-01T13:09:56Z (GMT). No. of bitstreams: 1 2007_dis_craabreu.pdf: 1760977 bytes, checksum: 9089d14f4e32ad467697dbb14559502f (MD5) Previous issue date: 2007 / Cashew has big importance in Brazilian culture of fruits, principally in Northwest region, presenting very expressive social and economic activity. Accordingly to some studies cashew apple has a high vitamin C, carotenoids and phenolics compounds contents. More than vitaminic potential, these compounds have antioxidant potential to cashew pulps. This work aimed at to evaluate the quality and the antioxidant capacity of peduncles from different commercials clones of early dwarf cashew apple tree, developed by Embrapa Agroindustria Tropical, destinated to in natura market and transformation industry, through of physical, chemistry and physical-chemical characterization, selecting the clones that presenting the best results. Ten clones, from Experimental Station of Embrapa Agroindustria Tropical, located in municipality of Pacajus-Ceara, were harvested: CCP-76, CCP-06, CCP-09, CCP-1001, BRS- 189, BRS-226, BRS-265, BRS Bahia 12, Embrapa 50 and Embrapa 51. The clones were characterized to: weight, length, color, firmness, pH, soluble solids (SS), total acidity (TA), SS/TA, total soluble sugars (TSS), vitamin C, anthocianins, yellow flavonoids, total carotenoids, condensed tannins (CT), total extractable polyphenols (TEP) and total antioxidant capacity (TAC). The peduncle presented high quality, according with the evaluated attributes, presenting raised variation between the clones, don’t being verified on pH parameter, without significative statistical difference (p<0,05), between the treatments. The most detachable clones were BRS 189 and BRS Bahia 12. The first one presented good physical characteristics like the firmness, the most attractive color, being a high source of bioactive compounds like vitamin C, total anthocianins and total carotenoids, presenting the highest values in these compounds. The second one has a good texture and is a high source of vitamin C and is a excellent source of polyphenols and presenting the most value of total antioxidant capacity, between the evaluated clones. Keywords: cashew, clones, quality, bioactivies compounds, total antioxidant capacity / A cajucultura tem grande importancia na fruticultura brasileira, principalmente para o Nordeste, pois representa uma atividade economica e social de grande expressao nessa regiao. Conforme estudos ja realizados, o pedunculo do cajueiro e rico em vitamina C, carotenoides e compostos fenolicos. Alem do potencial vitaminico, estes compostos conferem potencial antioxidante a polpa do caju. Com isso, esse experimento objetivou avaliar a qualidade e a atividade antioxidante total de pedunculos de diferentes clones comerciais de cajueiro anão precoce desenvolvidos pela Embrapa Agroindustria Tropical, destinados ao comercio in natura e a industria de transformacao, atraves da caracterizacao fisica, quimica e fisicoquimica, selecionando os clones que apresentaram os melhores resultados. Foram escolhidos dez clones, provenientes da Estacao Experimental da Embrapa Agroindustria Tropical, localizada no municipio de Pacajus - CE: CCP 76, CCP 06, CCP 09, CCP 1001, BRS 189, BRS 226, BRS 265, BRS Bahia 12, Embrapa 50 e Embrapa 51. Sendo caracterizados quanto a: peso, tamanho, coloracao, firmeza, pH, solidos soluveis (SS), acidez titulavel (AT), SS/AT, acucares soluveis totais (AST), vitamina C, antocianinas totais, flavonoides amarelos, carotenoides totais, taninos condensados (TC), polifenois extraiveis totais (PET) e atividade antioxidante total (AAT). Os pedunculos de cajueiro mostraram boa qualidade, de acordo com os atributos avaliados, apresentando uma variacao entre os clones, que so nao foi verificada na variavel pH, onde nao houve diferenca estatistica significativa (p<0,05), entre os tratamentos. Os clones que mais se destacaram foram o BRS 189 e o BRS Bahia 12, o primeiro, por apresentar boas caracteristicas fisicas, como a melhor firmeza, coloracao mais atrativa, alem de ser uma boa fonte de compostos bioativos como vitamina C, antocianinas totais e carotenoides totais, apresentando os maiores valores desses componentes. E o segundo, por possuir tambem uma boa firmeza e ser uma boa fonte de vitamina C e principalmente, por ser uma excelente fonte de polifenois e apresentar a maior atividade antioxidante total, dentre os clones avaliados. Palavras-chave: caju, clones, qualidade, compostos bioativos, atividade antioxidante total.

FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / A cajucultura tem grande importancia na fruticultura brasileira, principalmente para o Nordeste, pois representa uma atividade economica e social de grande expressao nessa regiao. Conforme estudos ja realizados, o pedunculo do cajueiro e rico em vitamina C, carotenoides e compostos fenolicos. Alem do potencial vitaminico, estes compostos conferem potencial antioxidante a polpa do caju. Com isso, esse experimento objetivou avaliar a qualidade e a atividade antioxidante total de pedunculos de diferentes clones comerciais de cajueiro anÃo precoce desenvolvidos pela Embrapa Agroindustria Tropical, destinados ao comercio in natura e a industria de transformacao, atraves da caracterizacao fisica, quimica e fisicoquimica, selecionando os clones que apresentaram os melhores resultados. Foram escolhidos dez clones, provenientes da Estacao Experimental da Embrapa Agroindustria Tropical, localizada no municipio de Pacajus - CE: CCP 76, CCP 06, CCP 09, CCP 1001, BRS 189, BRS 226, BRS 265, BRS Bahia 12, Embrapa 50 e Embrapa 51. Sendo caracterizados quanto a: peso, tamanho, coloracao, firmeza, pH, solidos soluveis (SS), acidez titulavel (AT), SS/AT, acucares soluveis totais (AST), vitamina C, antocianinas totais, flavonoides amarelos, carotenoides totais, taninos condensados (TC), polifenois extraiveis totais (PET) e atividade antioxidante total (AAT). Os pedunculos de cajueiro mostraram boa qualidade, de acordo com os atributos avaliados, apresentando uma variacao entre os clones, que so nao foi verificada na variavel pH, onde nao houve diferenca estatistica significativa (p<0,05), entre os tratamentos. Os clones que mais se destacaram foram o BRS 189 e o BRS Bahia 12, o primeiro, por apresentar boas caracteristicas fisicas, como a melhor firmeza, coloracao mais atrativa, alem de ser uma boa fonte de compostos bioativos como vitamina C, antocianinas totais e carotenoides totais, apresentando os maiores valores desses componentes. E o segundo, por possuir tambem uma boa firmeza e ser uma boa fonte de vitamina C e principalmente, por ser uma excelente fonte de polifenois e apresentar a maior atividade antioxidante total, dentre os clones avaliados. Palavras-chave: caju, clones, qualidade, compostos bioativos, atividade antioxidante total. / Cashew has big importance in Brazilian culture of fruits, principally in Northwest region, presenting very expressive social and economic activity. Accordingly to some studies cashew apple has a high vitamin C, carotenoids and phenolics compounds contents. More than vitaminic potential, these compounds have antioxidant potential to cashew pulps. This work aimed at to evaluate the quality and the antioxidant capacity of peduncles from different commercials clones of early dwarf cashew apple tree, developed by Embrapa Agroindustria Tropical, destinated to in natura market and transformation industry, through of physical, chemistry and physical-chemical characterization, selecting the clones that presenting the best results. Ten clones, from Experimental Station of Embrapa Agroindustria Tropical, located in municipality of Pacajus-Ceara, were harvested: CCP-76, CCP-06, CCP-09, CCP-1001, BRS- 189, BRS-226, BRS-265, BRS Bahia 12, Embrapa 50 and Embrapa 51. The clones were characterized to: weight, length, color, firmness, pH, soluble solids (SS), total acidity (TA), SS/TA, total soluble sugars (TSS), vitamin C, anthocianins, yellow flavonoids, total carotenoids, condensed tannins (CT), total extractable polyphenols (TEP) and total antioxidant capacity (TAC). The peduncle presented high quality, according with the evaluated attributes, presenting raised variation between the clones, donât being verified on pH parameter, without significative statistical difference (p<0,05), between the treatments. The most detachable clones were BRS 189 and BRS Bahia 12. The first one presented good physical characteristics like the firmness, the most attractive color, being a high source of bioactive compounds like vitamin C, total anthocianins and total carotenoids, presenting the highest values in these compounds. The second one has a good texture and is a high source of vitamin C and is a excellent source of polyphenols and presenting the most value of total antioxidant capacity, between the evaluated clones. Keywords: cashew, clones, quality, bioactivies compounds, total antioxidant capacity

Made available in DSpace on 2014-06-11T19:29:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-06-27Bitstream added on 2014-06-13T18:58:52Z : No. of bitstreams: 1 ferreira_rm_me_jabo.pdf: 499475 bytes, checksum: adcc6fc6d838f270b207706f21c18463 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Os objetivos do estudo foram: avaliar o efeito antioxidante do Trolox® 1) no meio de manutenção de embriões sobre as taxas de concepção (25 e 46 dias) e a perda gestacional de receptoras bovinas Holandesas repetidoras de serviço, em dois períodos (abril-junho/setembro-novembro) e 2) no meio de transporte de oócitos bovinos aspirados de ovários de abatedouro sobre as taxas de clivagem, blastocisto e eclosão in vitro. No Experimento 1, doadoras de embriões foram superovuladas e submetidas à lavagem uterina. Os embriões colhidos foram divididos em dois grupos, deixados em meio com ou sem antioxidante, por 2 a 6h. No Experimento 2, folículos foram aspirados e os complexos cumulus-oócito grau I e II foram divididos em quatro grupos: Controle 8h, Antioxidante 8h, Controle 20h e Antioxidante 20h. Então, foram mantidos em uma transportadora de oócitos (37ºC) por 8 ou 20 horas. Após a maturação, fecundação e cultivo in vitro, as taxas de clivagem (D3), blastocisto (D6, 7 e 9) e eclosão (D11) foram avaliadas. Alíquotas de 200 μL do meio de transporte foram retiradas em cada momento experimental (0, 8 e 20h) para realização dos testes de capacidade antioxidante total (CAT). No Experimento 1, não houve efeito de tratamento sobre as variáveis avaliadas. Foi verificado efeito de período experimental (P=0,001), categoria da doadora (P<0,05), qualidade embrionária (P=0,049) e intervalo Divisão dos Grupos-Inovulação (P<0,0001). No Experimento 2, não houve efeito de tratamento sobre as taxas analisadas. No entanto, houve redução das taxas de clivagem e blastocisto (D7 e 9) nos grupos 20 horas. Ainda, no D6 foram obtidas taxas de blastocisto semelhantes nos grupos Antioxidante 8 e 20h. A análise da CAT evidenciou que o Trolox® auxiliou o combate às ROS. / The objectives of the present study were to evaluate the effect of the addition of an antioxidant (Trolox®) 1) to a holding media for bovine in vivo produced embryos, on conception rates 25 and 46 days of pregnancy and pregnancy loss, in Holstein bovine recipients at 4th or more service, during two periods of the year (April-June/September-November) and 2) to a transport media for bovine oocytes aspirated from slaughter house ovaries, on in vitro cleavage, blastocyst and hatching rates. In Experiment 1, donor cows were superovulated and submitted to uterine flushings. The recovered embryos were divided into two groups, kept in holding media with or without antioxidant, for 2-6h. In Experiment 2, follicles were aspirated and cumulus-oocyte complexes grade I and II were divided into four groups: Control 8h, Antioxidant 8h, Control 20h and Antioxidant 20h. Oocytes were kept in a transportable machine (37ºC) for 8 or 20 hours. After in vitro maturation, fertilization and culture, cleavage (D3), blastocyst (D6, 7 and 9) and hatching rates (D11) were evaluated. Samples (200 μL) of the transport media were collected in each experimental moment (0, 8 e 20h) for total antioxidant capacity assay (TACA). In Experiment 1, no effect of treatment was observed. Effects of experimental period (P=0.001), donor category (P<0.05), embryo quality (P=0.049) and interval Group division-embryo transfer (P<0.0001) were detected. In Experiment 2, no effect of treatment was found on the analyzed rates. However, reduction on cleavage and blastocyst (D7 e 9) rates was detected on Groups 20h. Also, on D6 similar blastocyst rates were observed in Groups Antioxidant 8 and 20h. The analysis of TACA evidenced that Trolox® collaborated with the combat against the ROS.

Orientador: Paulo Henrique Franceschini / Banca: Antônio Cláudio Tedesco / Banca: Francisco Guilherme Leite / Resumo: Os objetivos do estudo foram: avaliar o efeito antioxidante do Trolox® 1) no meio de manutenção de embriões sobre as taxas de concepção (25 e 46 dias) e a perda gestacional de receptoras bovinas Holandesas repetidoras de serviço, em dois períodos (abril-junho/setembro-novembro) e 2) no meio de transporte de oócitos bovinos aspirados de ovários de abatedouro sobre as taxas de clivagem, blastocisto e eclosão in vitro. No Experimento 1, doadoras de embriões foram superovuladas e submetidas à lavagem uterina. Os embriões colhidos foram divididos em dois grupos, deixados em meio com ou sem antioxidante, por 2 a 6h. No Experimento 2, folículos foram aspirados e os complexos cumulus-oócito grau I e II foram divididos em quatro grupos: Controle 8h, Antioxidante 8h, Controle 20h e Antioxidante 20h. Então, foram mantidos em uma transportadora de oócitos (37ºC) por 8 ou 20 horas. Após a maturação, fecundação e cultivo in vitro, as taxas de clivagem (D3), blastocisto (D6, 7 e 9) e eclosão (D11) foram avaliadas. Alíquotas de 200 μL do meio de transporte foram retiradas em cada momento experimental (0, 8 e 20h) para realização dos testes de capacidade antioxidante total (CAT). No Experimento 1, não houve efeito de tratamento sobre as variáveis avaliadas. Foi verificado efeito de período experimental (P=0,001), categoria da doadora (P<0,05), qualidade embrionária (P=0,049) e intervalo Divisão dos Grupos-Inovulação (P<0,0001). No Experimento 2, não houve efeito de tratamento sobre as taxas analisadas. No entanto, houve redução das taxas de clivagem e blastocisto (D7 e 9) nos grupos 20 horas. Ainda, no D6 foram obtidas taxas de blastocisto semelhantes nos grupos Antioxidante 8 e 20h. A análise da CAT evidenciou que o Trolox® auxiliou o combate às ROS. / Abstract: The objectives of the present study were to evaluate the effect of the addition of an antioxidant (Trolox®) 1) to a holding media for bovine in vivo produced embryos, on conception rates 25 and 46 days of pregnancy and pregnancy loss, in Holstein bovine recipients at 4th or more service, during two periods of the year (April-June/September-November) and 2) to a transport media for bovine oocytes aspirated from slaughter house ovaries, on in vitro cleavage, blastocyst and hatching rates. In Experiment 1, donor cows were superovulated and submitted to uterine flushings. The recovered embryos were divided into two groups, kept in holding media with or without antioxidant, for 2-6h. In Experiment 2, follicles were aspirated and cumulus-oocyte complexes grade I and II were divided into four groups: Control 8h, Antioxidant 8h, Control 20h and Antioxidant 20h. Oocytes were kept in a transportable machine (37ºC) for 8 or 20 hours. After in vitro maturation, fertilization and culture, cleavage (D3), blastocyst (D6, 7 and 9) and hatching rates (D11) were evaluated. Samples (200 μL) of the transport media were collected in each experimental moment (0, 8 e 20h) for total antioxidant capacity assay (TACA). In Experiment 1, no effect of treatment was observed. Effects of experimental period (P=0.001), donor category (P<0.05), embryo quality (P=0.049) and interval Group division-embryo transfer (P<0.0001) were detected. In Experiment 2, no effect of treatment was found on the analyzed rates. However, reduction on cleavage and blastocyst (D7 e 9) rates was detected on Groups 20h. Also, on D6 similar blastocyst rates were observed in Groups Antioxidant 8 and 20h. The analysis of TACA evidenced that Trolox® collaborated with the combat against the ROS. / Mestre

Submitted by Geandra Rodrigues (geandrar@gmail.com) on 2018-01-29T18:04:49Z No. of bitstreams: 0 / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2018-03-21T19:28:36Z (GMT) No. of bitstreams: 0 / Made available in DSpace on 2018-03-21T19:28:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-08-24 / FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais / A esteatohepatite não alcoólica (EHNA) é uma manifestação da síndrome metabólica e distúrbios hepáticos, caracterizada por esteatose, inflamação lobular, hepatócitos edemaciados e citoplasma rarefeitos (balonização) e, em alguns casos, fibrose, que pode evoluir para a cirrose e carcinoma. A progressão da EHNA também está intimamente relacionada à resistência à insulina, quanto ao estresse oxidativo. A ingestão dietética de antioxidantes tem sido sugerida em proteção contra dano oxidativo e complicações clínicas relacionadas. A capacidade antioxidante total da dieta (CATd) é considerada uma ferramenta útil para avaliar o potencial antioxidante da alimentação, e não há nenhum estudo, atualmente, que examine a relação da CATd com a EHNA. O objetivo deste estudo foi avaliar a potencial associação da capacidade antioxidante total da dieta com severidade em pacientes com EHNA, bem como com composição antropométrica e marcadores corporal e parâmetros bioquímicos. Foram avaliados trinta e três pacientes com idade média de 48,4 ± 11,0 anos. Gravidade de EHNA, características de estilo de vida, ocorrência de comorbidades, ingestão dietética, antropometria, composição corporal e parâmetros bioquímicos foram avaliados. Observou-se que 24,2% (n=25) eram diabéticos tipo 2, 48,5% (n=17) possuíam hipertensão arterial sistêmica e 84,8% (n=28) eram dislipidêmicos. Em relação à obesidade, 81,8% (n=27) apresentavam obesidade central de risco e 84,8% apresentavam excesso de peso de acordo com o índice de massa corporal. A presença de síndrome metabólica foi notada em 54,5% (n=18) dos participantes. Os pacientes com EHNA que tiveram uma maior CAT dietética tiveram menos lesões nos hepatócitos (balonização) em comparação com aqueles com menor CATd (p = 0,034). Os pacientes com CAT dietético mais elevado teve uma redução de aproximadamente 20% no risco de ter muitos hepatócitos balonizados (odds ratio [OR]: 0.791; 95% intervalo de confiança [IC]: 0.643-0,974, p = 0,027). Não houve associação entre esteatose, inflamação lobular e fibrose com CAT dietético. O mesmo ocorreu com características de estilo de vida, ocorrência de comorbidades, antropometria, composição corporal e parâmetros bioquímicos. Conclui-se que CAT dietético é maior em pacientes com lesão hepática inferior (balonismo), sugerindo um possível papel de ingestão naturalmente alta de alimentos em sua capacidade antioxidante em reduzindo a produção de radicais livres e, consequentemente, o estresse oxidativo. / Nonalcoholic steatohepatitis (NASH) is a manifestation of the metabolic syndrome and hepatic disorders, characterized by steatosis, lobular inflammation, edema and rarefied cytoplasm (balonization) and, in some cases, fibrosis, which can progress to cirrhosis and carcinoma. The progression of NASH is also closely related to insulin resistance in relation to oxidative stress. Dietary intake of antioxidants has been suggested in protection against oxidative damage and related clinical complications. The total antioxidant capacity of the diet (CATd) is considered a useful tool to evaluate the antioxidant potential of the diet, and there is currently no study examining the relationship of CATd with NASH. The objective of this study was to evaluate the potential association of total antioxidant capacity of the diet with severity in patients with NASH, as well as with anthropometric composition and body markers and biochemical parameters. Thirty-three patients with a mean age of 48.4 ± 11.0 years were evaluated. EHNA severity, lifestyle characteristics, occurrence of comorbidities, dietary intake, anthropometry, body composition and biochemical parameters were evaluated. It was observed that 24.2% (n = 25) were diabetic type 2, 48.5% (n = 17) had systemic arterial hypertension and 84.8% (n = 28) were dyslipidemic. Regarding obesity, 81.8% (n = 27) had central obesity at risk and 84.8% were overweight according to the body mass index. The presence of metabolic syndrome was noted in 54.5% (n = 18) of the participants. Patients with NASH who had a higher dietary CAT had fewer hepatocyte (balloon) lesions compared to those with lower CATd (p = 0.034). Patients with higher dietary CAT had an approximately 20% reduction in the risk of having many balloonized hepatocytes (odds ratio [OR]: 0.791; 95% confidence interval [CI]: 0.643-0.974, p = 0.027). There was no association between steatosis, lobular inflammation and fibrosis with dietary CAT. The same occurred with characteristics of life style, occurrence of comorbidities, anthropometry, body composition and biochemical parameters. It is concluded that dietary CAT is greater in patients with lower hepatic injury (balloonism), suggesting a possible role of naturally high food intake in its antioxidant capacity in reducing the production of free radicals and, consequently, oxidative stress.

Chronic pancreatitis (CP) is a heterogeneous disease defined as chronic inflammatory changes of the pancreatic tissue caused by variety of aetiologies. Oxidative stress accompanying the inflammatory processes has been suggested as an important factor contributing to CP development. The aim of this study was to determine levels of lipid peroxidation products malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), together with nitrites, the total antioxidant capacity, cytokines, biochemical and haematological parameters in the plasma of patients with CP and control subjects. Levels of MDA and 4-HNE were analyzed using high-performance liquid chromatography. The total antioxidant capacity of plasma against peroxyl radicals was evaluated using chemiluminescence determination. Nitrites were determined using Griess reaction. Cytokines - TNF-alfa; TNF RI; PDGF-AB; TGF-beta, together with myeloperoxidase and hyaluronan were determined using ELISA Kits. Biochemical and haematological parameters were measured by standard methods.